keyword
MENU ▼
Read by QxMD icon Read
search

Epoxide hydrolase

keyword
https://www.readbyqxmd.com/read/28522175/pharmacological-inhibition-of-soluble-epoxide-hydrolase-or-genetic-deletion-reduces-diclofenac-induced-gastric-ulcers
#1
Sumanta Kumar Goswami, Amelia Ann Rand, Debin Wan, Jun Yang, Bora Inceoglu, Melany Thomas, Christophe Morisseau, Guang-Yu Yang, Bruce D Hammock
AIMS: This research was conducted to evaluate the hypothesis that gastric ulcers caused by the NSAID diclofenac sodium (DCF) can be prevented by the soluble epoxide hydrolase inhibitor TPPU. MAIN METHODS: Mice were administered a single dose of 10, 30 or 100mg/kg of DCF. Once an ulcerative dose of DCF was chosen, mice were pretreated with TPPU for 7days at 0.1mg/kg to evaluate anti-ulcer effects of the sEH inhibitor on anatomy, histopathology, pH, inflammatory markers and epithelial apoptosis of stomachs...
May 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28521794/in-vitro-synthesis-of-9-10-dihydroxyhexadecanoic-acid-using-recombinant-escherichia-coli
#2
Anees Kaprakkaden, Preeti Srivastava, Virendra Swarup Bisaria
BACKGROUND: Hydroxy fatty acids are widely used in food, chemical and cosmetic industries. A variety of dihydroxy fatty acids have been synthesized so far; however, no studies have been done on the synthesis of 9,10-dihydroxyhexadecanoic acid. In the present study recombinant E. coli has been used for the heterologous expression of fatty acid hydroxylating enzymes and the whole cell lysate of the induced culture was used for in vitro production of 9,10-dihydroxyhexadecanoic acid. RESULTS: A first of its kind proof of principle has been successfully demonstrated for the production of 9,10-dihydroxyhexadecanoic acid using three different enzymes viz...
May 18, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28502798/structure-of-a-soluble-epoxide-hydrolase-identified-in-trichoderma-reesei
#3
C Wilson, G S De Oliveira, P P Adriani, F S Chambergo, M V B Dias
Epoxide hydrolases (EHs) are enzymes that have high biotechnological interest for the fine and transformation industry. Several of these enzymes have enantioselectivity, which allows their application in the separation of enantiomeric mixtures of epoxide substrates. Although two different families of EHs have been described, those that have the α/β-hidrolase folding are the most explored for biotechnological purpose. These enzymes are functionally very well studied, but only few members have three-dimensional structures characterised...
May 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28501602/soluble-epoxide-hydrolase-inhibitory-activity-of-components-from-leonurus-japonicus
#4
Hyun Hee Leem, Ga Young Lee, Ji Sun Lee, Hanna Lee, Jang Hoon Kim, Young Ho Kim
One new compound, 10-methoxy-leonurine (1), and four known compounds (2-5) were purified by silica gel, C-18, and Sephadex LH-20 column chromatography from Leonurus japonicus. Their structures were elucidated using one-dimensional (1D)/two-dimensional (2D)-nuclear magnetic resonance (NMR), high-resolution (HR)-electrospray ionization (ESI) mass spectrometry (MS). The compounds were evaluated to determine their inhibition of the catalysis of soluble epoxide hydrolase (sEH). According to the results from in vitro analyses, compounds 1 and 2, which contain guanidine and flavonoid (3), were determined to be potential inhibitors of this enzyme...
May 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28497532/arabidopsis-thaliana-epoxide-hydrolase1-ateh1-is-a-cytosolic-epoxide-hydrolase-involved-in-the-synthesis-of-poly-hydroxylated-cutin-monomers
#5
Emmanuelle Pineau, Lin Xu, Hugues Renault, Adrien Trolet, Nicolas Navrot, Pascaline Ullmann, Bertrand Légeret, Gaëtan Verdier, Fred Beisson, Franck Pinot
Epoxide hydrolases (EHs) are present in all living organisms. They have been extensively characterized in mammals; however, their biological functions in plants have not been demonstrated. Based on in silico analysis, we identified AtEH1 (At3g05600), a putative Arabidopsis thaliana epoxide hydrolase possibly involved in cutin monomer synthesis. We expressed AtEH1 in yeast and studied its localization in vivo. We also analyzed the composition of cutin from A. thaliana lines in which this gene was knocked out...
May 12, 2017: New Phytologist
https://www.readbyqxmd.com/read/28492351/mest-a-unique-epoxide-hydrolase-is-essential-for-optimal-growth-of-mycobacterium-tuberculosis-in-the-presence-of-styrene-oxide
#6
Manisha Chownk, Aashish Sharma, Kashmir Singh, Jagdeep Kaur
AIM: mesT of Mycobacterium tuberculosis, a hypothetical/putative epoxide hydrolase, is predicted to convert toxic epoxides to the more water-soluble and less toxic diols. Detailed characterization of the protein was carried out. RESULTS: mesT demonstrated esterase as well as epoxide hydrolase activity. It was membrane bound and was upregulated under hypoxic conditions. The enzyme was able to degrade styrene oxide. The presence of antisense against this gene resulted in the inhibition of in vitro bacterial growth/survival in the presence of styrene oxide...
May 11, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28488585/eets-reduces-lps-induced-hyperpermeability-by-targeting-grp78-mediated-src-activation-and-subsequent-rho-rock-signaling-pathway
#7
Ruolan Dong, Danli Hu, Yan Yang, Zhihui Chen, Menglu Fu, Dao Wen Wang, Xizhen Xu, Ling Tu
Integrity of endothelial barrier is a determinant of the prognosis in the acute lung injury caused by sepsis. The epoxyeicosatrienoic acids (EETs), metabolites of arachidonic acid, exhibit protective effects in various pathogenic states, however, whether EETs play a role in endothelial barrier enhancement and the involved mechanisms remain to be investigated. Here, we show that increased EETs level by endothelial specific cytochrome P450 epoxygenase 2J2 over-expression and soluble epoxide hydrolase (sEH) inhibitor TPPU reduced lipopolysaccharide-induced endothelial hyper-permeability in vivo, accompanied by improved survival of septic mice...
April 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/28485854/podocyte-specific-soluble-epoxide-hydrolase-deficiency-in-mice-attenuates-acute-kidney-injury
#8
Ahmed Bettaieb, Shinichiro Koike, Samah Chahed, Yi Zhao, Santana Bachaalany, Nader Hashoush, James Graham, Fatima Huma, Peter J Havel, Artiom Gruzdev, Darryl C Zeldin, Bruce D Hammock, Fawaz G Haj
Podocytes play an important role in maintaining glomerular function, and podocyte injury is a significant component in the pathogenesis of proteinuria. Soluble epoxide hydrolase (sEH) is a cytosolic enzyme whose genetic deficiency and pharmacological inhibition have beneficial effects on renal function, but its role in podocytes remains unexplored. The objective of this study was to investigate the contribution of sEH in podocytes to lipopolysaccharide (LPS)-induced kidney injury. We report increased sEH transcript and protein expression in murine podocytes upon LPS challenge...
May 9, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28473204/inhibition-of-soluble-epoxide-hydrolase-reduces-portal-pressure-by-protecting-mesenteric-artery-myogenic-responses-in-cirrhotic-rats
#9
Yijun Huang, Jun Qin, Dong Sun, Houli Jiang, Lei Zheng, Yue He, Liang Gui, Binbin Qian, Chihao Zhang, Meng Luo
Hyperdynamic circulation contributes to the progress of portal hypertension in liver cirrhosis. We investigated the effects of soluble epoxide hydrolase (sEH) inhibition on portal pressure and the myogenic response of mesenteric arteries isolated from cirrhotic rats using the sEH inhibitor t-TUCB (trans-4-{4-[3-(4-trifluoromethoxyphenyl)-ureido] cyclohexyloxy} benzoic acid). Cirrhotic tissues had a higher ratio of epoxyeicosatrienoic acids (EETs) to dihydroxyeicosatrienoic acids (DHETs) following increased CYP2C11 expression, which may be a protective response...
May 1, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/28472063/occurrence-of-urea-based-soluble-epoxide-hydrolase-inhibitors-from-the-plants-in-the-order-brassicales
#10
Seiya Kitamura, Christophe Morisseau, Todd R Harris, Bora Inceoglu, Bruce D Hammock
Recently, dibenzylurea-based potent soluble epoxide hydrolase (sEH) inhibitors were identified in Pentadiplandra brazzeana, a plant in the order Brassicales. In an effort to generalize the concept, we hypothesized that plants that produce benzyl glucosinolates and corresponding isothiocyanates also produce these dibenzylurea derivatives. Our overall aim here was to examine the occurrence of urea derivatives in Brassicales, hoping to find biologically active urea derivatives from plants. First, plants in the order Brassicales were analyzed for the presence of 1, 3-dibenzylurea (compound 1), showing that three additional plants in the order Brassicales produce the urea derivatives...
2017: PloS One
https://www.readbyqxmd.com/read/28460522/nanobody-based-immunoassay-for-human-soluble-epoxide-hydrolase-detection-using-polymeric-horseradish-peroxidase-polyhrp-for-signal-enhancement-the-rediscovery-of-polyhrp
#11
Dongyang Li, Yongliang Cui, Christophe Morisseau, Shirley J Gee, Candace S Bever, Xiangjiang Liu, Jian Wu, Bruce D Hammock, Yibin Ying
Soluble epoxide hydrolase (sEH) is a potential pharmacological target for treating hypertension, vascular inflammation, cancer, pain, and multiple cardiovascular related diseases. A variable domain of the heavy chain antibody (termed single domain antibody (sdAb), nanobody, or VHH) possesses the advantages of small size, high stability, ease of genetic manipulation, and ability for continuous manufacture, making such nanobody a superior choice as an immunoreagent. In this work, we developed an ultrasensitive nanobody based immunoassay for human sEH detection using polymeric horseradish peroxidase (PolyHRP) for signal enhancement...
May 10, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28454544/attenuation-of-acute-lung-injury-in-a-rat-model-by-semen-cassiae
#12
Xiuqing Chen, Xianming Zhang, Jie Zhang, Yang Gao, Zhaohui Yang, Shanshan Li, Haiwen Dai
BACKGROUND: Acute lung injury (ALI) is an inflammatory disorder. Semen Cassiae has potent anti-inflammatory activities. The aim of our study was to investigate whether Semen Cassiae plays a protective effect on lipopolysaccharide (LPS)-induced ALI and, if so, to elucidate its potential mechanism. METHODS: Male Sprague-Dawley rat lungs were injured by intratracheal instillation of LPS. Rats were treated with Semen Cassiae or vehicle 3 h after LPS challenge. Samples were harvested 24 h post-LPS administration...
April 28, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28450284/eets-promote-hypoxic-pulmonary-vasoconstriction-via-constrictor-prostanoids
#13
Sharath Kandhi, Bin Zhang, Ghezal Froogh, Jun Qin, Norah Alruwali, Yicong Le, Yang-Ming Yang, Sung Hee Hwang, Bruce D Hammock, Michael S Wolin, An Huang, Dong Sun
To test the hypothesis that epoxyeicosatrienoic acids (EETs) facilitate pulmonary responses to hypoxia, male wild type (WT) and soluble epoxide hydrolase knockout (sEH-KO) mice, and WT mice chronically fed a sEH inhibitor (t-TUCB; 1mg/kg/day) were used. Right ventricular systolic pressure (RVSP) was recorded under control and hypoxic conditions. The control RVSP was comparable among all groups. However, hypoxia elicited increases in RVSP in all groups with predominance in sEH-KO and t-TUCB-treated mice. 14,15-EEZE (EET antagonist) attenuated the hypoxia-induced greater elevation of RVSP in sEH-deficient mice, suggesting an EET-mediated increment...
April 27, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28442442/prostaglandins-and-other-lipid-mediators-polm-special-issue-of-the-6th-european-workshop-on-lipid-mediators-role-of-m%C3%A3-ller-cell-cytochrome-p450-2c44-in-murine-retinal-angiogenesis
#14
Jiong Hu, Alexandra Geyer, Sarah Dziumbla, Khader Awwad, Darryl C Zeldin, Wolf-Hagen Schunck, Rüdiger Popp, Timo Frömel, Ingrid Fleming
Polyunsaturated fatty acids (PUFA) and their cytochrome P450 (CYP450) metabolites have been linked to angiogenesis and vessel homeostasis. However, the role of individual CYP isoforms and their endogenous metabolites in those processes are not clear. Here, we focused on the role of Cyp2c44 in postnatal retinal angiogenesis and report that Cyp2c44 is highly expressed in Müller glial cells in the retina. The constitutive as well as inducible postnatal genetic deletion of Cyp2c44 resulted in an increased vessel network density without affecting vessel radial expansion during the first postnatal week...
April 22, 2017: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/28428366/in-vitro-metabolism-of-oprozomib-an-oral-proteasome-inhibitor-role-of-epoxide-hydrolases-and-cytochrome-p450s
#15
Zhican Wang, Ying Fang, Juli Teague, Hansen Wong, Christophe Morisseau, Bruce D Hammock, Dan A Rock, Zhengping Wang
Oprozomib is an oral proteasome inhibitor currently under investigation in patients with hematologic malignancies or solid tumors. Oprozomib elicits potent pharmacological actions by forming a covalent bond with the active site N-terminal threonine of the 20S proteasome. Oprozomib has a short half-life across preclinical species and in patients due to systemic clearance via metabolism. Potential for drug-drug interactions (DDIs) could alter the exposure of this potent therapeutic therefore a thorough investigation of pathways responsible for metabolism is required...
April 20, 2017: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/28408149/leptinotarsa-cap-n-collar-isoform-c-kelch-like-ech-associated-protein%C3%A2-1-signaling-is-critical-for-the-regulation-of-ecdysteroidogenesis-in-the-larvae
#16
Qiang-Kun Sun, Qing-Wei Meng, Qing-Yu Xu, Pan Deng, Wen-Chao Guo, Guo-Qing Li
Drosophila cap 'n' collar isoform C (CncC) and Kelch-like ECH associated protein 1 (Keap1) regulate metamorphosis by transcriptional control of a subset of genes involved in ecdysteroidogenesis, 20-hydroxyecdysone (20E) signaling, and juvenile hormone (JH) degradation. In the present paper, we found that prothoracicotropic hormone signal was required for the activation of LdCncC and LdKeap1 in Leptinotarsa decemlineata. Moreover, RNA interference of LdCncC or LdKeap1 in the fourth-instar larvae delayed development...
April 10, 2017: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/28403941/obesity-is-positively-associated-with-arachidonic-acid-derived-5-and-11-hydroxyeicosatetraenoic-acid-hete
#17
Charles Austin Pickens, Lorraine M Sordillo, Chen Zhang, Jenifer I Fenton
BACKGROUND: Oxylipids are oxygenated polyunsaturated fatty acid (PUFA) metabolites that are responsible for the onset and resolution of the inflammatory response. Enzymatic oxygenation through the lipoxygenase (LOX) or cytochrome P450 (CYP) pathways can form oxylipids that have either proinflammatory or proresolving functions depending on the type of PUFA substrate and degree of metabolism. The objective of this study was to determine how PUFA substrates and their corresponding oxylipids are associated with obesity...
May 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28400585/juvenile-hormone-epoxide-hydrolase-a-promising-target-for-hemipteran-pest-management
#18
Abudourusuli Tusun, Ming Li, Xiangzhi Liang, Ting Yang, Bin Yang, Guirong Wang
Juvenile hormone epoxide hydrolase (JHEH) has attracted great interest because of its critical role in the regulation of juvenile hormone (JH) in insects. In this study, one JHEH gene from Apolygus lucorum (AlucJHEH) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. The results reveals a conserved catalytic mechanism of AlucJHEH toward JH. Our study also demonstrates that the mRNA of AlucJHEH gene was detectable in head, thorax and abdomen from all life stages...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28396419/cyclooxygenase-derived-proangiogenic-metabolites-of-epoxyeicosatrienoic-acids
#19
Amy A Rand, Bogdan Barnych, Christophe Morisseau, Tomas Cajka, Kin Sing Stephen Lee, Dipak Panigrahy, Bruce D Hammock
Arachidonic acid (ARA) is metabolized by cyclooxygenase (COX) and cytochrome P450 to produce proangiogenic metabolites. Specifically, epoxyeicosatrienoic acids (EETs) produced from the P450 pathway are angiogenic, inducing cancer tumor growth. A previous study showed that inhibiting soluble epoxide hydrolase (sEH) increased EET concentration and mildly promoted tumor growth. However, inhibiting both sEH and COX led to a dramatic decrease in tumor growth, suggesting that the contribution of EETs to angiogenesis and subsequent tumor growth may be attributed to downstream metabolites formed by COX...
April 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28392259/active-site-flexibility-and-substrate-specificity-in-a-bacterial-virulence-factor-crystallographic-snapshots-of-an-epoxide-hydrolase
#20
Kelli L Hvorecny, Christopher D Bahl, Seiya Kitamura, Kin Sing Stephen Lee, Bruce D Hammock, Christophe Morisseau, Dean R Madden
Pseudomonas aeruginosa secretes an epoxide hydrolase with catalytic activity that triggers degradation of the cystic fibrosis transmembrane conductance regulator (CFTR) and perturbs other host defense networks. Targets of this CFTR inhibitory factor (Cif) are largely unknown, but include an epoxy-fatty acid. In this class of signaling molecules, chirality can be an important determinant of physiological output and potency. Here we explore the active-site chemistry of this two-step α/β-hydrolase and its implications for an emerging class of virulence enzymes...
May 2, 2017: Structure
keyword
keyword
9146
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"