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https://www.readbyqxmd.com/read/29931263/single-molecule-microscopy-reveals-dynamic-flna-interactions-governing-sstr2-clustering-and-internalization
#1
Donatella Treppiedi, Marie-Lise Jobin, Erika Peverelli, Elena Giardino, Titiwat Sungkaworn, Ulrike Zabel, Maura Arosio, Anna Spada, Giovanna Mantovani, Davide Calebiro
The cytoskeletal protein filamin A (FLNA) has been suggested to play an important role in the responsiveness of GH-secreting pituitary tumors to somatostatin receptor subtype 2 (SSTR2) agonists, by regulating SSTR2 expression and signaling. However, the underlying mechanisms are unknown. Here, we use fast multi-color single-molecule microscopy to image individual SSTR2 and FLNA molecules at the surface of living cells with unprecedented spatiotemporal resolution. We find that SSTR2 and FLNA undergo transient interactions, which occur preferentially along actin fibers and contribute to restraining SSTR2 diffusion...
June 19, 2018: Endocrinology
https://www.readbyqxmd.com/read/29931117/foliar-application-of-zinc-sulfate-and-zinc-edta-to-wheat-leaves-differences-in-mobility-distribution-and-speciation
#2
C L Doolette, T L Read, C Li, K G Scheckel, E Donner, P M Kopittke, J K Schjoerring, E Lombi
Foliar application of zinc (Zn) to crops is an effective way to increase the grain concentration of Zn. However, the development of more efficient foliar Zn fertilisers is limited by a lack of knowledge regarding the distribution, mobility and speciation of Zn in leaves once it is taken up by the plant. We performed an experiment using radiolabelled Zn (65Zn), and in situ time resolved elemental imaging using synchrotron X-ray fluorescence microscopy (XFM), to investigate the behaviour of two commonly used Zn foliar fertilisers (Zn sulfate and ZnEDTA) in wheat (Triticum aestivum) leaves...
June 21, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29927745/history-of-disruptions-in-laboratory-medicine-what-have-we-learned-from-predictions
#3
REVIEW
Larry J Kricka
Predictions about the future of laboratory medicine have had a mixed success, and in some instances they have been overambitious and incorrectly assessed the future impact of emerging technologies. Current predictions suggest a more highly automated and connected future for diagnostic testing. The central laboratory of the future may be dominated by more robotics and more connectivity in order to take advantage of the benefits of the Internet of Things and artificial intelligence (AI)-based systems (e.g. decision support software and imaging analytics)...
June 21, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29925779/accurate-smartphone-indoor-visual-positioning-based-on-a-high-precision-3d-photorealistic-map
#4
Teng Wu, Jingbin Liu, Zheng Li, Keke Liu, Beini Xu
Indoor positioning is in high demand in a variety of applications, and indoor environment is a challenging scene for visual positioning. This paper proposes an accurate visual positioning method for smartphones. The proposed method includes three procedures. First, an indoor high-precision 3D photorealistic map is produced using a mobile mapping system, and the intrinsic and extrinsic parameters of the images are obtained from the mapping result. A point cloud is calculated using feature matching and multi-view forward intersection...
June 20, 2018: Sensors
https://www.readbyqxmd.com/read/29920799/high-mobility-p-type-and-n-type-copper-nitride-semiconductors-by-direct-nitriding-synthesis-and-in-silico-doping-design
#5
Kosuke Matsuzaki, Kou Harada, Yu Kumagai, Shogo Koshiya, Koji Kimoto, Shigenori Ueda, Masato Sasase, Akihiro Maeda, Tomofumi Susaki, Masaaki Kitano, Fumiyasu Oba, Hideo Hosono
Thin-film photovoltaics (PV) have emerged as a technology that can meet the growing demands for efficient and low-cost large-scale cells. However, the photoabsorbers currently in use contain expensive or toxic elements, and the difficulty in bipolar doping, particularly in a device structure, requires elaborate optimization of the heterostructures for improving the efficiency. This study shows that bipolar doping with high hole and electron mobilities in copper nitride (Cu3 N), composed solely of earth-abundant and environmentally benign elements, is readily available through a novel gaseous direct nitriding reaction applicable to uniform and large-area deposition...
June 19, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29915877/distinguishing-acute-from-chronic-aortic-dissections-using-ct-imaging-features
#6
Norman A Orabi, Leslie E Quint, Kuanwong Watcharotone, Bin Nan, David M Williams, Karen M Kim
The aim was to compare computed tomography (CT) features in acute and chronic aortic dissections (AADs and CADs) and determine if a certain combination of imaging features was reliably predictive of the acute versus chronic nature of disease in individual patients. Consecutive patients with aortic dissection and a chest CT scan were identified, and 120 CT scans corresponding to 105 patients were reviewed for a variety of imaging features. Statistical tests assessed for differences in the frequency of these features...
June 18, 2018: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/29915802/laparoscopic-ovarian-transposition-prior-to-pelvic-radiation-for-gynecologic-cancer
#7
Brenna E Swift, Eric Leung, Danielle Vicus, Allan Covens
This study evaluates a novel technique of laparoscopic ovarian transposition performed by Gynecologic Oncologists prior to pelvic radiation for gynecologic cancer. A retrospective review was completed of all patients that underwent laparoscopic ovarian transposition from February 2007 to June 2017 at one tertiary care cancer. The technique involves salpingectomy, followed by retroperitoneal dissection to move the ovaries lateral to the hepatic and splenic flexures of the colon. Normal ovarian function was defined by the absence of vasomotor symptoms, FSH and menstrual history (if menstruating)...
May 2018: Gynecologic Oncology Reports
https://www.readbyqxmd.com/read/29913935/adult-limb-and-breast-amputees-experience-and-descriptions-of-phantom-phenomena-a-qualitative-study
#8
Berit Björkman, Staffan Arnér, Iréne Lund, Lars-Christer Hydén
Background Phantom phenomena - pain or other sensations appearing to come from amputated body parts - are frequent consequences of amputation and can cause considerable suffering. Also, stump pain, located in the residual limb, is in the literature often related to the phantom phenomena. The condition is not specific to amputated limbs and has, to a lesser extent, been reported to be present after radical surgery in other body parts such as breast, rectum and teeth. Multi-causal theories are used when trying to understand these phenomena, which are recognized as the result of complex interaction among various parts of the central nervous system confirmed in studies using functional brain imaging techniques...
December 29, 2017: Scandinavian Journal of Pain
https://www.readbyqxmd.com/read/29910420/do-image-assisted-mobile-applications-improve-dietary-habits-knowledge-and-behaviours-in-elite-athletes-a-pilot-study
#9
Anne Simpson, Luke Gemming, Dane Baker, Andrea Braakhuis
To date, there has been a paucity of research on optimal ways to educate and promote dietary behavioural change within athletes. Optimising athlete nutrition is fundamental to reaching peak performance and maintaining athlete wellbeing. MealLogger® is a smartphone application that incorporates the use of an image-based food record and social-media functionality to provide in-application personalised feedback to individuals or groups, peer-support, and a platform to deliver nutrition education material. This study measured the feasibility of MealLogger® within New Zealand elite male field hockey players (n = 17) aged 18⁻20 to increase athlete knowledge and nutrition promoting behaviours...
August 11, 2017: Sports
https://www.readbyqxmd.com/read/29909185/accuracy-of-smartphone-based-evaluation-of-emergent-large-vessel-occlusion-on-cta
#10
Douglas T Hidlay, Ryan A McTaggart, Grayson Baird, Shadi Yaghi, Morgan Hemendinger, Eric L Tung, Eleanor L Dibiasio, Richard A Haas, Mahesh V Jayaraman
OBJECTIVES: Mechanical thrombectomy is the standard of care for stroke caused by an emergent large vessel occlusion in the anterior circulation, and the ability to rapidly review CTA is one hurdle in minimizing the time from diagnosis to intervention. We evaluated the diagnostic accuracy and confidence in review of stroke CTA for ELVO via a smartphone-based application as compared to PACS workstation. PATIENTS AND METHODS: Seventy-six head and neck CTA studies performed for stroke from one comprehensive and seven primary stroke centers were independently reviewed remotely on smartphone by two blinded interventional neuroradiologists in actual-use circumstances...
June 11, 2018: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29906672/displacement-of-teeth-without-and-with-bonded-fixed-orthodontic-retainers-3d-analysis-using-triangular-target-frames-and-optoelectronic-motion-tracking-device
#11
Firas Chakroun, Vera Colombo, Dave Lie Sam Foek, Luigi Maria Gallo, Albert Feilzer, Mutlu Özcan
PURPOSE: The objective of this study was to evaluate the anterior tooth movement without and with bonded fixed orthodontic retainers under incremental loading conditions. MATERIALS AND METHODS: Six extracted mandibular anterior human teeth were embedded in acrylic resin in True Form I Arch type and 3D reconstruction of Digital Volume Tomography (DVT) images (0.4 mm3 voxels) were obtained. The anatomy of each tooth was segmented and digitally reconstructed using 3D visualization software for medical images (AMIRA, FEI SVG)...
June 6, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29906015/randomized-and-controlled-pilot-study-of-the-pragmatic-use-of-mobile-phone-based-follow-up-of-actinic-keratoses-treated-with-topical-5-fluorouracil
#12
Suzana S Bosanac, Victoria Nguyen, Donna Bui, Daniel B Eisen, Raja K Sivamani
Store-and-forward teledermatology involves transmission of a patient's images to a healthcare provider and subsequent response from the provider about the diagnosis or management. Furthermore, teledermatology in which mobile phones (e.g. smartphones) are utilized for communication between the patient and their provider is referred to as mobile-teledermatology. In this study, we investigate the use of mobile-teledermatology in the management of actinic keratoses. We demonstrate that mobile-teledermatology may enhance communication between the patient and their provider when managing cutaneous disease and that even individuals in older age groups are highly satisfied with this type of follow up...
April 15, 2018: Dermatology Online Journal
https://www.readbyqxmd.com/read/29904747/a-rapid-computational-approach-for-assessing-interfraction-esophageal-motion-for-use-in-stereotactic-body-radiation-therapy-planning
#13
Michael L Cardenas, Thomas R Mazur, Christina I Tsien, Olga L Green
Purpose: We present a rapid computational method for quantifying interfraction motion of the esophagus in patients undergoing stereotactic body radiation therapy on a magnetic resonance (MR) guided radiation therapy system. Methods and materials: Patients who underwent stereotactic body radiation therapy had simulation computed tomography (CT) and on-treatment MR scans performed. The esophagus was contoured on each scan. CT contours were transferred to MR volumes via rigid registration...
April 2018: Advances in Radiation Oncology
https://www.readbyqxmd.com/read/29903495/mobilityanalyser-a-novel-approach-for-automatic-quantification-of-cell-mobility-on-periodic-patterned-substrates-using-brightfield-microscopy-images
#14
Ângela Carvalho, Tiago Esteves, Pedro Quelhas, Fernando Jorge Monteiro
BACKGROUND AND OBJECTIVE: Surface topography of biomaterials has been shown to have an effect on cells behaviour. Cell-material interactions can be visually characterized by assessing both cell shape and spreading at initial time-points and, its migration patterns, as a response to the underlying topography. Whilst many have reported the study of cell migration and shape with fluorescence labelling, the focus on evaluating cells response to surface topography is to observe, under real-time conditions, interactions between cells and surfaces...
August 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29903480/a-mobile-computer-aided-system-for-optic-nerve-head-detection
#15
Yaroub Elloumi, Mohamed Akil, Nasser Kehtarnavaz
BACKGROUND AND OBJECTIVE: The detection of optic nerve head (ONH) in retinal fundus images plays a key role in identifying Diabetic Retinopathy (DR) as well as other abnormal conditions in eye examinations. This paper presents a method and its associated software towards the development of an Android smartphone app based on a previously developed ONH detection algorithm. The development of this app and the use of the d-Eye lens which can be snapped onto a smartphone provide a mobile and cost-effective computer-aided diagnosis (CAD) system in ophthalmology...
August 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29902815/feasibility-of-transabdominal-doppler-sonography-for-studying-uterine-blood-flow-characteristics-in-cycling-gilts
#16
Catherine Herlta, Rosa Starka, Haukur L Sigmarsson, Johannes Kauffold
OBJECTIVE: To test for the feasibility of transabdominal Doppler sonography (color, power, pulse wave) to define uterine perfusion characteristics throughout the estrous cycle in gilts. MATERIALS AND METHODS: A total of 15 gilts were synchronized for estrus and scanned in their following spontaneous cycle while being restrained in a purpose-designed mobile crate. To define uterine perfusion characteristics, vessels in between and within uterine cross-sections were imaged and recorded as video sequences to be analyzed by PixelFlux® software for perfused area (Amix), blood flow velocity (vmix) and intensity (Imix) as well as resistance (RIvmix) and pulsatility index (PIvmix)...
June 2018: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/29902758/characterization-of-phosphorus-availability-in-response-to-radial-oxygen-losses-in-the-rhizosphere-of-vallisneria-spiralis
#17
Chao Han, Jinghua Ren, Zhaode Wang, Shika Yang, Fan Ke, Di Xu, Xianchuan Xie
The viewpoint that radial oxygen loss (ROL) of submerged macrophytes induces changes in redox conditions and the associated phosphorus (P) availability has been indirectly confirmed at larger spatial scales using conventional, destructive techniques. However, critical information about microniches has largely been overlooked due to the lack of satisfactory in situ mapping technologies. In this study, we deployed a recently developed hybrid sensor in the rhizosphere of Vallisneria spiralis (V. spiralis) during two vegetation periods to provide 2-D imaging of the spatiotemporal co-distribution of oxygen (O2 ) and P from a fixed observation point...
June 1, 2018: Chemosphere
https://www.readbyqxmd.com/read/29901776/easyfrap-web-a-web-based-tool-for-the-analysis-of-fluorescence-recovery-after-photobleaching-data
#18
Grigorios Koulouras, Andreas Panagopoulos, Maria A Rapsomaniki, Nickolaos N Giakoumakis, Stavros Taraviras, Zoi Lygerou
Understanding protein dynamics is crucial in order to elucidate protein function and interactions. Advances in modern microscopy facilitate the exploration of the mobility of fluorescently tagged proteins within living cells. Fluorescence recovery after photobleaching (FRAP) is an increasingly popular functional live-cell imaging technique which enables the study of the dynamic properties of proteins at a single-cell level. As an increasing number of labs generate FRAP datasets, there is a need for fast, interactive and user-friendly applications that analyze the resulting data...
June 13, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29901537/an-evidence-based-approach-to-the-management-of-children-with-morquio-a-syndrome-presenting-with-craniocervical-pathology
#19
Nicole Williams, Alessandro Narducci, Deborah M Eastwood, Maureen Cleary, Dominic Thompson
STUDY DESIGN: Retrospective case series OBJECTIVE.: To review clinical and radiological outcomes of craniovertebral surgery in children with Morquio A syndrome (Mucopolysaccharidosis type IVA) and develop an evidence-based management algorithm. SUMMARY OF BACKGROUND DATA: Myelopathy secondary to craniovertebral pathology is a common cause of neurological disability in Morquio A syndrome. Previously unresolved surgical controversies include the value of surveillance, surgical indications and operative technique...
June 12, 2018: Spine
https://www.readbyqxmd.com/read/29899053/live-cell-imaging-of-mobile-rnas-in-plants
#20
Leonor C Boavida
No abstract text is available yet for this article.
June 2018: Plant Physiology
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