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https://www.readbyqxmd.com/read/28443167/elderly-patients-achieving-clinical-and-radiological-outcomes-comparable-with-those-of-younger-patients-following-minimally-invasive-transforaminal-lumbar-interbody-fusion
#1
Joshi Nikhil N, Joel Wei-An Lim, William Yeo, Wai-Mun Yue
STUDY DESIGN: Retrospective analysis of prospective database. PURPOSE: To compare 2-year clinical and radiological outcomes after minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) among "middle-age" (50-64.99 years), "young-old" (65-74.99 years), and "old-old" (>75 years) patients. OVERVIEW OF LITERATURE: Owing to higher perioperative morbidity and mortality rates, elderly patients with degenerative lumbar conditions are occasionally denied surgical care, even after conservative treatment failure...
April 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/28442456/the-malaria-system-microapp-a-new-mobile-device-based-tool-for-malaria-diagnosis
#2
Allisson Dantas Oliveira, Clara Prats, Mateu Espasa, Francesc Zarzuela Serrat, Cristina Montañola Sales, Aroa Silgado, Daniel Lopez Codina, Mercia Eliane Arruda, Jordi Gomez I Prat, Jones Albuquerque
BACKGROUND: Malaria is a public health problem that affects remote areas worldwide. Climate change has contributed to the problem by allowing for the survival of Anopheles in previously uninhabited areas. As such, several groups have made developing news systems for the automated diagnosis of malaria a priority. OBJECTIVE: The objective of this study was to develop a new, automated, mobile device-based diagnostic system for malaria. The system uses Giemsa-stained peripheral blood samples combined with light microscopy to identify the Plasmodium falciparum species in the ring stage of development...
April 25, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28439942/assessment-of-motion-of-colonic-contents-in-the-human-colon-using-mri-tagging
#3
S E Pritchard, J Paul, G Major, L Marciani, P A Gowland, R C Spiller, C L Hoad
BACKGROUND: We have previously reported a non-invasive, semi-automated technique to assess motility of the wall of the ascending colon (AC) using Magnetic Resonance Imaging. This study investigated the feasibility of using a tagged MRI technique to visualize and assess the degree of flow within the human ascending colon in healthy subjects and those suffering from constipation. METHODS: An open-labeled study of 11 subjects with constipation and 11 subjects without bowel disorders was performed...
April 25, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28438609/novel-replicons-and-trans-encapsidation-systems-for-hepatitis-c-virus-proteins-live-imaging-and-virus-host-interaction-proteomics
#4
Ovidiu Vlaicu, Tudor Selescu, Florin Pastrama, Cristian Munteanu, Laura Riva, Jean Dubuisson, Yves Rouille, Costin-Ioan Popescu
Proteomics and imaging techniques are used more and more in tandem to investigate the virus-host interaction. Herein we present novel replicons, methods and trans-encapsidation systems suitable for determination of Hepatitis C Virus (HCV) proteins interactomes and live imaging of viral proteins dynamics in HCV cell culture (HCVcc) system. To identify endogenous factors involved in the HCV life cycle, we constructed full-length functional replicons with affinity purification (AP) tags fused to NS2 and NS5A proteins...
April 21, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28437353/evaluation-of-the-modern-luque-trolley-construct-for-the-treatment-of-early-onset-scoliosis-using-a-gliding-implant-in-an-immature-animal-model
#5
Jean A Ouellet, Catherine E Ferland, Karina Klein, Guillaume Racloz, Karina Klein, Henning Richter, Thomas Steffen, Brigitte von Rechenberg
STUDY DESIGN: This was an experimental animal study. OBJECTIVE: To determine biological compatibility, stability, and growth potential of the Trolley Gliding Vehicle (TGV) used in a novel surgical technique for guided spinal growth. SUMMARY OF BACKGROUND DATA: Current treatments for early-onset scoliosis maintaining spinal growth consist of posteriorly based spinal constructs requiring repetitive lengthening. Such interventions have a high rate of complications...
May 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28436842/a-hybrid-image-registration-and-matching-framework-for-real-time-motion-tracking-in-mri-guided-radiotherapy
#6
Matteo Seregni, Chiara Paganelli, Paul Summers, Massimo Bellomi, Guido Baroni, Marco Riboldi
OBJECTIVE: MRI-guided radiotherapy (MRIgRT) is an emerging treatment technique where anatomical and pathological structures are imaged through integrated MR-radiotherapy units. This work aims (i) at assessing the accuracy of optical-flow based motion tracking in liver cine-MRI sequences and (ii) at testing a MRIgRT workflow combining similarity-based image matching with image registration. METHODS: After an initialization stage, a set of template images is collected and registered to the first frame of the cine-MRI sequence...
April 24, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28436531/international-inter-rater-agreement-in-scoring-acne-severity-utilizing-cloud-based-image-sharing-of-mobile-phone-photographs
#7
Negar Foolad, Jennifer N Ornelas, Ashley K Clark, Ifrah Ali, Victoria R Sharon, Luluah Al Mubarak, Andrés Lopez, Ali Alikhan, Bishr Al Dabagh, Alireza Firooz, Smita Awasthi, Yu Liu, Chin-Shang Li, Raja K Sivamani
BACKGROUND: Cloud-based image sharing technology allows facilitated sharing of images. Cloud-based image sharing technology has not been well-studied for acne assessments or treatment preferences, among international evaluators. We evaluated inter-rater variability of acne grading and treatment recommendations among an international group of dermatologists that assessed photographs. METHODS: This is a prospective, single visit photographic study to assess inter-rater agreement of acne photographs shared through an integrated mobile device, cloud-based, and HIPAA-compliant platform...
April 24, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28436155/the-mobius-airo-mobile-ct-for-image-guided-proton-therapy-characterization-commissioning
#8
Jasmine A Oliver, Omar A Zeidan, Sanford L Meeks, Amish P Shah, Jason Pukala, Patrick Kelly, Naren R Ramakrishna, Twyla R Willoughby
PURPOSE: The purpose of this study was to characterize the Mobius AIRO Mobile CT System for localization and image-guided proton therapy. This is the first known application of the AIRO for proton therapy. METHODS: Five CT images of a Catphan(®) 504 phantom were acquired on the AIRO Mobile CT System, Varian EDGE radiosurgery system cone beam CT (CBCT), Philips Brilliance Big Bore 16 slice CT simulator, and Siemens SOMATOM Definition AS 20 slice CT simulator. DoseLAB software v...
April 24, 2017: Journal of Applied Clinical Medical Physics
https://www.readbyqxmd.com/read/28433532/exci-cest-exploiting-pharmaceutical-excipients-as-mri-cest-contrast-agents-for-tumor-imaging
#9
Dario Livio Longo, Fatima Zzahra Moustaghfir, Alexandre Zerbo, Lorena Consolino, Annasofia Anemone, Martina Bracesco, Silvio Aime
Chemical Exchange Saturation Transfer (CEST) approach is a novel tool within magnetic resonance imaging (MRI) that allows visualization of molecules possessing exchangeable protons with water. Many molecules, employed as excipients for the formulation of finished drug products, are endowed with hydroxyl, amine or amide protons, thus can be exploitable as MRI-CEST contrast agents. Their high safety profiles allow them to be injected at very high doses. Here we investigated the MRI-CEST properties of several excipients (ascorbic acid, sucrose, N-acetyl-d-glucosamine, meglumine and 2-pyrrolidone) and tested them as tumor-detecting agents in two different murine tumor models (breast and melanoma cancers)...
April 19, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28432787/presynaptic-morphology-and-vesicular-composition-determine-vesicle-dynamics-in-mouse-central-synapses
#10
Laurent Guillaud, Dimitar Dimitrov, Tomoyuki Takahashi
Transport of synaptic vesicles (SVs) in nerve terminals is thought to play essential roles in maintenance of neurotransmission. To identify factors modulating SV movements, we performed real-time imaging analysis of fluorescently labeled SVs in giant calyceal and conventional hippocampal terminals. Compared with small hippocampal terminals, SV movements in giant calyceal terminals were faster, longer and kinetically more heterogeneous. Morphological maturation of giant calyceal terminals was associated with an overall reduction in SV mobility and displacement heterogeneity...
April 22, 2017: ELife
https://www.readbyqxmd.com/read/28432403/bone-marrow-fat-content-in-70-adolescent-girls-with-anorexia-nervosa-magnetic-resonance-imaging-and-magnetic-resonance-spectroscopy-assessment
#11
Kirsten Ecklund, Sridhar Vajapeyam, Robert V Mulkern, Henry A Feldman, Jennifer M O'Donnell, Amy D DiVasta, Catherine M Gordon
BACKGROUND: Adolescents and women with anorexia nervosa have increased bone marrow fat and decreased bone formation, at least in part due to hormonal changes leading to preferential stem cell differentiation to adipocytes over osteoblasts. OBJECTIVE: The purpose of this study was to evaluate marrow fat content and correlate with age and disease severity using knee MRI with T1 relaxometry (T1-R) and MR spectroscopy (MRS) in 70 adolescents with anorexia nervosa. MATERIALS AND METHODS: We enrolled 70 girls with anorexia nervosa who underwent 3-T knee MRI with coronal T1-W images, T1-R and single-voxel proton MRS at 30 and 60 ms TE...
April 22, 2017: Pediatric Radiology
https://www.readbyqxmd.com/read/28428367/copii-coated-membranes-function-as-transport-carriers-of-intracellular-procollagen-i
#12
Amita Gorur, Lin Yuan, Samuel J Kenny, Satoshi Baba, Ke Xu, Randy Schekman
The coat protein complex II (COPII) is essential for the transport of large cargo, such as 300-nm procollagen I (PC1) molecules, from the endoplasmic reticulum (ER) to the Golgi. Previous work has shown that the CUL3-KLHL12 complex increases the size of COPII vesicles at ER exit sites to more than 300 nm in diameter and accelerates the secretion of PC1. However, the role of large COPII vesicles as PC1 transport carriers was not unambiguously demonstrated. In this study, using stochastic optical reconstruction microscopy, correlated light electron microscopy, and live-cell imaging, we demonstrate the existence of mobile COPII-coated vesicles that completely encapsulate the cargo PC1 and are physically separated from ER...
April 20, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28424248/splenic-ly6g-high-mature-and-ly6g-int-immature-neutrophils-contribute-to-eradication-of-s-pneumoniae
#13
Justin F Deniset, Bas G Surewaard, Woo-Yong Lee, Paul Kubes
The spleen plays an integral protective role against encapsulated bacterial infections. Our understanding of the associated mechanisms is limited to thymus-independent (TI) antibody production by the marginal zone (MZ) B cells, leaving the contribution of other splenic compartments such as the red pulp (RP) largely unexplored despite asplenic patients succumbing to the infection in the first 24 h, suggesting important antibody-independent mechanisms. In this study, using time-lapse intravital imaging of the spleen, we identify a tropism for Streptococcus pneumoniae in this organ mediated by tissue-resident MZ and RP macrophages and a protective role for two distinct splenic neutrophil populations (Ly6G(hi) and Ly6G(intermediate)) residing in the splenic RP...
April 19, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28418620/fast-and-highly-sensitive-ionic-polymer-gated-ws2-graphene-photodetectors
#14
Jake D Mehew, Selim Unal, Elias Torres Alonso, Gareth F Jones, Saad Fadhil Ramadhan, Monica F Craciun, Saverio Russo
The combination of graphene with semiconductor materials in heterostructure photodetectors enables amplified detection of femtowatt light signals using micrometer-scale electronic devices. Presently, long-lived charge traps limit the speed of such detectors, and impractical strategies, e.g., the use of large gate-voltage pulses, have been employed to achieve bandwidths suitable for applications such as video-frame-rate imaging. Here, atomically thin graphene-WS2 heterostructure photodetectors encapsulated in an ionic polymer are reported, which are uniquely able to operate at bandwidths up to 1...
April 18, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28414927/integration-of-free-hand-3d-ultrasound-and-mobile-c-arm-cone-beam-ct-feasibility-and-characterization-for-real-time-guidance-of-needle-insertion
#15
E Marinetto, A Uneri, T De Silva, S Reaungamornrat, W Zbijewski, A Sisniega, S Vogt, G Kleinszig, J Pascau, J H Siewerdsen
This work presents development of an integrated ultrasound (US)-cone-beam CT (CBCT) system for image-guided needle interventions, combining a low-cost ultrasound system (Interson VC 7.5MHz, Pleasanton, CA) with a mobile C-arm for fluoroscopy and CBCT via use of a surgical tracker. Imaging performance of the ultrasound system was characterized in terms of depth-dependent contrast-to-noise ratio (CNR) and spatial resolution. US-CBCT system was evaluated in phantom studies simulating three needle-based procedures: drug delivery, tumor ablation, and lumbar puncture...
April 3, 2017: Computerized Medical Imaging and Graphics: the Official Journal of the Computerized Medical Imaging Society
https://www.readbyqxmd.com/read/28414097/single-molecule-and-super-resolution-imaging-of-transcription-in-living-bacteria
#16
REVIEW
Mathew Stracy, Achillefs N Kapanidis
In vivo single-molecule and super-resolution techniques are transforming our ability to study transcription as it takes place in its native environment in living cells. This review will detail the methods for imaging single molecules in cells, and the data-analysis tools which can be used to extract quantitative information on the spatial organization, mobility, and kinetics of the transcription machinery from these experiments. Furthermore, we will highlight studies which have applied these techniques to shed new light on bacterial transcription...
April 13, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28413806/ryanodine-and-ip3-receptor-mediated-calcium-signaling-play-a-pivotal-role-in-neurological-infrared-laser-modulation
#17
Gleb P Tolstykh, Cory A Olsovsky, Bennett L Ibey, Hope T Beier
Pulsed infrared (IR) laser energy has been shown to modulate neurological activity through both stimulation and inhibition of action potentials. While the mechanism(s) behind this phenomenon is (are) not completely understood, certain hypotheses suggest that the rise in temperature from IR exposure could activate temperature- or pressure-sensitive ion channels or create pores in the cellular outer membrane, allowing an influx of typically plasma-membrane-impermeant ions. Studies using fluorescent intensity-based calcium ion ([Formula: see text]) sensitive dyes show changes in [Formula: see text] levels after various IR stimulation parameters, which suggests that [Formula: see text] may originate from the external solution...
April 2017: Neurophotonics
https://www.readbyqxmd.com/read/28409038/a-foreign-body-granuloma-of-the-buccal-mucosa-induced-by-honeybee-sting
#18
Kazuhiko Yamamoto, Yohei Nakayama, Yumiko Matsusue, Miyako Kurihara, Takahiro Yagyuu, Tadaaki Kirita
A foreign body granuloma of the buccal mucosa induced by honeybee sting was reported. The patient was an 82-year-old female who presented with a submucous mass at the right buccal mucosa. The mass was 20 mm in diameter, elastically firm, partly mobile without pain or tenderness, and covered with almost normal mucosa. MR image did not delineate the lesion clearly. Under clinical diagnosis of a benign tumor, the lesion was excised under local anesthesia. The excised lesion was 14 × 11 × 9 mm in size and solid and yellowish in cut surface...
2017: Case Reports in Dentistry
https://www.readbyqxmd.com/read/28402890/cholesterol-regulates-monocyte-rolling-through-cd44-distribution
#19
Amit K Saha, Pawel Osmulski, Shatha F Dallo, Maria Gaczynska, Tim H-M Huang, Anand K Ramasubramanian
Cholesterol is an important risk factor of atherosclerosis, due to its active uptake by monocytes/macrophages. Monocyte recruitment from flowing blood to atherosclerotic foci is the key first step in the development of atherosclerosis. Cholesterol content alters cell membrane stiffness, and lateral lipid and protein diffusion. We hypothesized that cholesterol content will modulate the recruitment of monocytes to inflamed endothelial surface by altering the dynamics of adhesion receptors. We depleted or enriched the cellular cholesterol levels using methyl-β-cyclodextran in freshly isolated human monocytes...
April 11, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28398256/registration-of-vehicle-borne-point-clouds-and-panoramic-images-based-on-sensor-constellations
#20
Lianbi Yao, Hangbin Wu, Yayun Li, Bin Meng, Jinfei Qian, Chun Liu, Hongchao Fan
A mobile mapping system (MMS) is usually utilized to collect environmental data on and around urban roads. Laser scanners and panoramic cameras are the main sensors of an MMS. This paper presents a new method for the registration of the point clouds and panoramic images based on sensor constellation. After the sensor constellation was analyzed, a feature point, the intersection of the connecting line between the global positioning system (GPS) antenna and the panoramic camera with a horizontal plane, was utilized to separate the point clouds into blocks...
April 11, 2017: Sensors
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