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26S proteasome

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https://www.readbyqxmd.com/read/28928219/o2-sensing-associated-glycosylation-exposes-the-f-box-combining-site-of-the-dictyostelium-skp1-subunit-in-e3-ubiquitin-ligases
#1
M Osman Sheikh, David Thieker, Gordon Chalmers, Christopher M Schafer, Mayumi Ishihara, Parastoo Azadi, Robert J Woods, John N Glushka, Brad Bendiak, James H Prestegard, Christopher M West
Skp1 is a conserved protein linking cullin-1 to F-box proteins in SCF (Skp1-Cullin1-F-box) E3 ubiquitin ligases, which modify protein substrates with polyubiquitin chains that typically target them for 26S proteasome-mediated degradation. In Dictyostelium (a social amoeba), Toxoplasma gondii (the agent for human toxoplasmosis), and other protists, Skp1 is regulated by a unique pentasaccharide attached to hydroxylated Pro-143 within its C-terminal F-box binding domain. Prolyl hydroxylation of Skp1 contributes to O2-dependent Dictyostelium development, but full glycosylation at that position is required for optimal O2 sensing...
September 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28919033/non-26s-proteasome-endomembrane-trafficking-pathways-in-aba-signaling
#2
REVIEW
Feifei Yu, Qi Xie
The phytohormone abscisic acid (ABA) is a vital endogenous messenger that regulates diverse physiological processes in plants. The regulation of ABA signaling has been well studied at both the transcriptional and translational levels. Post-translational modification of key regulators in ABA signaling by the 26S ubiquitin proteasome pathway is well known. Recently, increasing evidence demonstrates that atypical turnover of key regulators by the endocytic trafficking pathway and autophagy also play vital roles in ABA perception, signaling, and action...
September 14, 2017: Trends in Plant Science
https://www.readbyqxmd.com/read/28911816/lead-discovery-and-chemical-biology-approaches-targeting-the-ubiquitin-proteasome-system
#3
REVIEW
Favour A Akinjiyan, Seth Carbonneau, Nathan T Ross
Protein degradation is critical for proteostasis, and the addition of polyubiquitin chains to a substrate is necessary for its recognition by the 26S proteasome. Therapeutic intervention in the ubiquitin proteasome system has implications ranging from cancer to neurodegeneration. Novel screening methods and chemical biology tools for targeting E1-activating, E2-conjugating and deubiquitinating enzymes will be discussed in this review. Approaches for targeting E3 ligase-substrate interactions as well as the proteasome will also be covered, with a focus on recently described approaches...
August 30, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28900483/sulforaphane-induced-apoptosis-via-promotion-of-mitochondrial-fusion-and-erk1-2-mediated-26s-proteasome-degradation-of-novel-pro-survival-bim-and-upregulation-of-bax-in-human-non-small-cell-lung-cancer-cells
#4
Yang Geng, Yan Zhou, Sai Wu, Yabin Hu, Kai Lin, Yalin Wang, Zhongnan Zheng, Wei Wu
Previous studies in our laboratory showed that sulforaphane (SFN) induced apoptosis by sustained activation of extracellular regulated protein kinases 1/2 (ERK1/2). However, the underlying mechanisms associated with SFN-induced apoptosis and downstream cascades which are modulated by ERK1/2 were not elucidated. Herein we demonstrated for the first time that alteration of mitochondrial dynamics contributed to SFN-induced apoptosis in human non-small cell lung cancer (NSCLC) cells. Reports showed that protein Bim not only induced apoptosis but also promoted proliferation under certain circumstances...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28898250/effects-of-proteasome-inhibitor-mg-132-on-the-parasite-schistosoma-mansoni
#5
Enyara R Morais, Katia C Oliveira, Renato G de Paula, Alice M M Ornelas, Érika B C Moreira, Fernanda Rafacho Badoco, Lizandra G Magalhães, Sergio Verjovski-Almeida, Vanderlei Rodrigues
Proteasome is a proteolytic complex responsible for intracellular protein turnover in eukaryotes, archaea and in some actinobacteria species. Previous work has demonstrated that in Schistosoma mansoni parasites, the proteasome inhibitor MG-132 affects parasite development. However, the molecular targets affected by MG-132 in S. mansoni are not entirely known. Here, we used expression microarrays to measure the genome-wide changes in gene expression of S. mansoni adult worms exposed in vitro to MG-132, followed by in silico functional analyses of the affected genes using Ingenuity Pathway Analysis (IPA)...
2017: PloS One
https://www.readbyqxmd.com/read/28893904/anthrax-lethal-toxin-rapidly-reduces-c-jun-levels-by-inhibiting-c-jun-gene-transcription-and-promoting-c-jun-protein-degradation
#6
Weiming Ouyang, Pengfei Guo, Hui Fang, David M Frucht
Anthrax is a life threatening disease caused by infection with Bacillus anthracis, which expresses lethal factor and the receptor-binding protective antigen. These two proteins combine to form anthrax lethal toxin (LT), whose proximal targets are mitogen activated kinase kinases (MKKs). However, the downstream mediators of LT toxicity remain elusive. We herein report that LT exposure rapidly reduces the levels of cJun, a key regulator of cell proliferation and survival. Blockade of proteasome dependent protein degradation with the 26S proteasome inhibitor MG132 largely restored cJun protein levels, suggesting that LT promotes degradation of cJun protein...
September 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28865087/the-arabidopsis-u-box-e3-ubiquitin-ligase-pub30-negatively-regulates-salt-tolerance-by-facilitating-bri1-kinase-inhibitor-1-bki1-degradation
#7
Ming Zhang, Jinfeng Zhao, Long Li, Yanan Gao, Linlin Zhao, Suyash Bhimgonda Patil, Jingjing Fang, Wenhui Zhang, Yuhong Yang, Ming Li, Xueyong Li
The Arabidopsis U-box E3 ubiquitin (Ub) ligases play an important role in the ubiquitin/26S proteasome-mediated protein degradation pathway. Recently PUB30 has been reported to participate in the salt stress response during seed germination stage in ABA-independent manner, but the molecular mechanism remains to be elucidated. Here we displayed that the pub30 mutant was more tolerant to salt stress during seed germination, whereas the mutant of its closest homologue PUB31 showed mild sensitivity to salt stress...
September 2, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28852398/how-does-conserved-dopamine-neurotrophic-factor-protect-against-and-rescue-neurodegeneration-of-pc12-cells
#8
Jia-Ming Mei, Chao-Shi Niu
Conserved dopamine neurotrophic factor protects and rescues dopaminergic neurodegeneration induced by 6-hydroxydopamine in vivo, but its potential value in treating Parkinson's disease remains controversial. Here, we used the proteasome inhibitors lactacystin and MG132 to induce neurodegeneration of PC12 cells. Afterwards, conserved dopamine neurotrophic factor was administrated as a therapeutic factor, both pretreatment and posttreatment. Our results showed that (1) conserved dopamine neurotrophic factor enhanced lactacystin/MG132-induced cell viability and morphology, and attenuated alpha-synuclein accumulation in differentiated PC12 cells...
July 2017: Neural Regeneration Research
https://www.readbyqxmd.com/read/28844860/an-aaa-motor-driven-mechanical-switch-in-rpn11-controls-deubiquitination-at-the-26s-proteasome
#9
Evan J Worden, Ken C Dong, Andreas Martin
Poly-ubiquitin chains direct protein substrates to the 26S proteasome, where they are removed by the deubiquitinase Rpn11 during ATP-dependent substrate degradation. Rapid deubiquitination is required for efficient degradation but must be restricted to committed substrates that are engaged with the ATPase motor to prevent premature ubiquitin chain removal and substrate escape. Here we reveal the ubiquitin-bound structure of Rpn11 from S. cerevisiae and the mechanisms for mechanochemical coupling of substrate degradation and deubiquitination...
September 7, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28837777/arsenite-binds-to-the-zinc-finger-motif-of-tip60-histone-acetyltransferase-and-induces-its-degradation-via-the-26s-proteasome
#10
Lok Ming Tam, Ji Jiang, Pengcheng Wang, Lin Li, Weili Miao, Xuejiao Dong, Yinsheng Wang
Arsenic is a ubiquitous environmental contaminant with widespread public health concern. Epidemiological studies have revealed that chronic human exposure to arsenic in drinking water is associated with the prevalence of skin, lung, and bladder cancers. Aberrant histone modifications (e.g., methylation, acetylation, and ubiquitination) were previously found to be accompanied by arsenic exposure; thus, perturbation of epigenetic pathways is thought to contribute to arsenic carcinogenesis. Arsenite is known to interact with zinc finger motifs of proteins, and zinc finger motif is present in and indispensable for the enzymatic activities of crucial histone-modifying enzymes especially the MYST family of histone acetyltransferases (e...
September 7, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/28837065/arabidopsis-e3-ubiquitin-ligases-pub22-and-pub23-negatively-regulate-drought-tolerance-by-targeting-aba-receptor-pyl9-for-degradation
#11
Jinfeng Zhao, Linlin Zhao, Ming Zhang, Syed Adeel Zafar, Jingjing Fang, Ming Li, Wenhui Zhang, Xueyong Li
Drought causes osmotic stress and rapidly triggers abscisic acid (ABA) accumulation in plants. The roles of various ABA receptors in drought tolerance and molecular mechanisms regulating ABA receptor stability needs to be elucidated. Here, we report that Arabidopsis plants overexpressing PYL9, one of the 14 pyrabactin resistance (PYR)/pyrabactin resistance-like (PYL)/regulatory component of ABA receptors (RCAR) family ABA receptors, gained drought tolerance trait. Osmotic stress induced accumulation of the PYL9 protein, which was regulated by the 26S proteasome...
August 24, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28821611/the-proteasome-interacting-ecm29-protein-disassembles-the-26s-proteasome-in-response-to-oxidative-stress
#12
Xiaorong Wang, Ilan E Chemmama, Clinton Yu, Alexander Huszagh, Yue Xu, Rosa Viner, Sarah Ashley Block, Peter Cimermancic, Scott D Rychnovsky, Yihong Ye, Andrej Sali, Lan Huang
Oxidative stress has been implicated in multiple human neurological and other disorders. Proteasomes are multi-subunit proteases critical for the removal of oxidatively damaged proteins. To understand stress-associated human pathologies, it is important to uncover the molecular events underlying the regulation of proteasomes upon oxidative stress. To this end, we investigated H2O2 stress-induced molecular changes of the human 26S proteasome and determined that stress-induced 26S proteasome disassembly is conserved from yeast to human...
August 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28818370/overexpression-of-vpeifp1-a-novel-f-box-kelch-repeat-protein-from-wild-chinese-vitis-pseudoreticulata-confers-higher-tolerance-to-powdery-mildew-by-inducing-thioredoxin-z-proteolysis
#13
Jie Wang, Wenkong Yao, Lei Wang, Fuli Ma, Weihuo Tong, Chen Wang, Rui Bao, Changyue Jiang, Yazhou Yang, Jianxia Zhang, Yan Xu, Xiping Wang, Chaohong Zhang, Yuejin Wang
An F-box protein (VpEIFP1) induced by Erysiphe necator was isolated from Vitis pseudoreticulata, a wild Chinese grapevine species naturally resistant to powdery mildew (PM). It contains an F-box domain and two Kelch-repeat motifs. Expression profiles indicate the VpEIFP1 is strongly induced at both transcriptional and translational levels by PM infection. A subcellular localisation assay showed that VpEIFP1 is predominantly located in the nucleus and cytoplasm. Overexpression of VpEIFP1 accelerated the accumulation of hydrogen peroxide (H2O2) and up-regulated the expressions of ICS2, NPR1 and PR1 involved in defence responses, resulting in suppression of PM germination and growth...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28797244/a-lowered-26s-proteasome-activity-correlates-with-mantle-lymphoma-cell-lines-resistance-to-genotoxic-stress
#14
Khaoula Ben Younes, Simon Body, Élodie Costé, Pierre-Julien Viailly, Hadjer Miloudi, Clémence Coudre, Fabrice Jardin, Fatma Ben Aissa-Fennira, Brigitte Sola
BACKGROUND: Mantle cell lymphoma (MCL) is a B-cell hemopathy characterized by the t(11;14) translocation and the aberrant overexpression of cyclin D1. This results in an unrestrained cell proliferation. Other genetic alterations are common in MCL cells such as SOX11 expression, mutations of ATM and/or TP53 genes, activation of the NF-κB signaling pathway and NOTCH receptors. These alterations lead to the deregulation of the apoptotic machinery and resistance to drugs. We observed that among a panel of MCL cell lines, REC1 cells were resistant towards genotoxic stress...
August 10, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28783511/antithrombin-debrecen-p-leu205pro-clinical-and-molecular-characterization-of-a-novel-mutation-associated-with-severe-thrombotic-tendency
#15
Anna Selmeczi, Réka Gindele, Péter Ilonczai, Attila Fekete, István Komáromi, Ágota Schlammadinger, Katalin Rázsó, Kitti B Kovács, Helga Bárdos, Róza Ádány, László Muszbek, Zsuzsanna Bereczky, Zoltán Boda, Zsolt Oláh
INTRODUCTION: Hereditary antithrombin (AT) deficiency is a rare thrombophilic disorder with heterogeneous genetic background and various clinical presentations. In this study we identified a novel AT mutation. Genotype-phenotype correlations, molecular characteristics and thrombotic manifestations of the mutation were investigated. MATERIALS AND METHODS: Thirty-one members of a single family were included. Clinical data was collected regarding thrombotic history...
July 24, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28766720/molecular-characterization-of-rice-arsenic-induced-ring-finger-e3-ligase-2-osair2-and-its-heterogeneous-overexpression-in-arabidopsis-thalaiana
#16
Sun-Goo Hwang, Sandeep Chapagain, A-Reum Han, Yong Chan Park, Hyeon Mi Park, Yong Hwan Kim, Cheol Seong Jang
Arsenic (As) accumulation adversely affects the growth and productivity of plants and poses a serious threat to human health and food security. In this study, we identified one As-responsive Really Interesting New Gene (RING) E3 ubiquitin ligase gene from rice root tissues during As stress. We named it Oryza sativa Arsenic-Induced RING E3 ligase 2 (OsAIR2). Expression of OsAIR2 was induced under various abiotic stress conditions, including heat, salt, drought, and As exposure. Results of an in vitro ubiquitination assay showed that OsAIR2 possesses an E3 ligase activity...
August 2, 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28765280/the-exocyst-subunit-sec3-is-regulated-by-a-protein-quality-control-pathway
#17
Caroline Kampmeyer, Antonina Karakostova, Signe M Schenstrøm, Amanda B Abildgaard, Anne-Marie Lauridsen, Isabelle Jourdain, Rasmus Hartmann-Petersen
Exocytosis involves fusion of secretory vesicles with the plasma membrane, thereby delivering membrane proteins to the cell surface and releasing material into the extracellular space. The tethering of the secretory vesicles before membrane fusion is mediated by the exocyst, an essential phylogenetically conserved octameric protein complex. Exocyst biogenesis is regulated by several processes, but the mechanisms by which the exocyst is degraded are unknown. Here, to unravel the components of the exocyst degradation pathway, we screened for extragenic suppressors of a temperature-sensitive fission yeast strain mutated in the exocyst subunit Sec3 (sec3-913)...
September 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28757562/unusual-antioxidant-properties-of-26s-proteasome-isolated-from-cold-adapted-organisms
#18
Marta Gogliettino, Ennio Cocca, Carmela Fusco, Bruna Agrillo, Alessia Riccio, Marco Balestrieri, Angelo Facchiano, Antonio Pepe, Gianna Palmieri
The oxidative challenge represents an important factor affecting the adaptive strategies in Antarctic fish, but their impact on the protein degradation machinery still remains unclear. The previous analysis of the first 26S proteasome from the Antarctic red-blooded fish Trematomus bernacchii, evidenced improved antioxidant functions necessary to counteract the environmental pro-oxidant conditions. The purpose of this work was to carry out a study on 26S proteasomes from the temperate red-blooded Dicenthrarcus labrax and the icefish Chionodraco hamatus in comparison with the isoform already described from T...
July 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28748595/genes-important-for-survival-or-reproduction-in-varroa-destructor-identified-by-rnai
#19
Zachary Y Huang, Guowu Bian, Zhiyong Xi, Xianbing Xie
The Varroa mite, (Varroa destructor), is the worst threat to honey bee health worldwide. To explore the possibility of using RNAi to control this pest, we determined the effects of knocking down various genes on Varroa mite survival and reproduction. Double-stranded RNA (dsRNA) of six candidate genes (Da, Pros26S, RpL8, RpL11, RpP0 and RpS13) were synthesized and each injected into Varroa mites, then mite survival and reproduction were assessed. Injection of dsRNA for Da (Daughterless) and Pros26S (Proteasome 26S subunit ATPase) caused a significant reduction in mite survival, with 3...
July 27, 2017: Insect Science
https://www.readbyqxmd.com/read/28728908/the-proteasome-maturation-protein-pomp-increases-proteasome-assembly-and-activity-in-psoriatic-lesional-skin
#20
Barbara A Zieba, Laurent Henry, Matthieu Lacroix, Mohamed Jemaà, Thierry Lavabre-Bertrand, Laurent Meunier, Olivier Coux, Pierre-Emmanuel Stoebner
BACKGROUND: The ubiquitin proteasome pathway is involved in the pathogenesis of psoriasis and proteasome subunits are increased in lesional psoriatic skin. Recent works have highlighted that proteasome levels can be regulated through modulation of proteasome assembly notably by the proteasome maturation protein POMP. OBJECTIVES: To investigate whether proteasome assembly and POMP expression are modified in psoriatic skin. METHODS: Proteasome assembly as well as expression of proteasome regulators were assessed in non-lesional and lesional psoriatic skin using native gel electrophoresis and western blots respectively...
April 30, 2017: Journal of Dermatological Science
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