keyword
MENU ▼
Read by QxMD icon Read
search

Biology pacing

keyword
https://www.readbyqxmd.com/read/27913843/molecular-evolution-in-historical-perspective
#1
Edna Suárez-Díaz
In the 1960s, advances in protein chemistry and molecular genetics provided new means for the study of biological evolution. Amino acid sequencing, nucleic acid hybridization, zone gel electrophoresis, and immunochemistry were some of the experimental techniques that brought about new perspectives to the study of the patterns and mechanisms of evolution. New concepts, such as the molecular evolutionary clock, and the discovery of unexpected molecular phenomena, like the presence of repetitive sequences in eukaryotic genomes, eventually led to the realization that evolution might occur at a different pace at the organismic and the molecular levels, and according to different mechanisms...
December 2, 2016: Journal of Molecular Evolution
https://www.readbyqxmd.com/read/27908674/renaissance-of-protein-crystallization-and-precipitation-in-biopharmaceuticals-purification
#2
REVIEW
Raquel Dos Santos, Ana Luísa Carvalho, A Cecília A Roque
The current chromatographic approaches used in protein purification are not keeping pace with the increasing biopharmaceutical market demand. With the upstream improvements, the bottleneck shifted towards the downstream process. New approaches rely in Anything But Chromatography methodologies and revisiting former techniques with a bioprocess perspective. Protein crystallization and precipitation methods are already implemented in the downstream process of diverse therapeutic biological macromolecules, overcoming the current chromatographic bottlenecks...
November 28, 2016: Biotechnology Advances
https://www.readbyqxmd.com/read/27900742/-precision-nursing-individual-based-knowledge-translation
#3
Li-Chi Chiang, Mei-Ling Yeh, Sui-Lung Su
U.S. President Obama announced a new era of precision medicine in the Precision Medicine Initiative (PMI). This initiative aims to accelerate the progress of personalized medicine in light of individual requirements for prevention and treatment in order to improve the state of individual and public health. The recent and dramatic development of large-scale biologic databases (such as the human genome sequence), powerful methods for characterizing patients (such as genomics, microbiome, diverse biomarkers, and even pharmacogenomics), and computational tools for analyzing big data are maximizing the potential benefits of precision medicine...
December 2016: Hu Li za Zhi the Journal of Nursing
https://www.readbyqxmd.com/read/27898247/challenges-for-the-maintenance-and-cryopreservation-of-multiple-isolates-of-model-microorganisms-an-example-using-the-marine-diatom-skeletonema-marinoi
#4
John G Day, Simon Tytor, Jenny Egardt, Monica Applegren, Cecilia Rad-Menéndez, Olga Chepurnova, Wim Vyverman, Anna Godhe
Modern genomic and metabolomic tools have provided the possibility of generating and interrogating large datasets that can provide answers to previously imponderable taxonomic, evolutionary, ecological, and physiological questions. However, the curatorial tools needed to provide and maintain the relevant biological resources on which new knowledge can be built have not kept pace with this meteoric rise in scientific capacity, its associated activity, or the huge increase in published science. The availability of biological material of guaranteed identity and quality in Biological Resource Centers is fundamental for scientific research, but it crucially depends on there being adequate preservation/maintenance methods that are capable of ensuring phenotypic, genotypic, and functional security of the biological material(s)...
November 29, 2016: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/27897454/considerations-for-standardizing-predictive-molecular-pathology-for-cancer-prognosis
#5
Michelangelo Fiorentino, Marina Scarpelli, Antonio Lopez-Beltran, Liang Cheng, Rodolfo Montironi
Molecular tests that were once ancillary to the core business of cyto-histopathology are becoming the third most relevant workload in pathology departments after histopathology/cytopathology and before autopsies. This has resulted from innovations in molecular biology techniques, which have developed at an incredibly fast pace. Areas covered: Most of the current widely used techniques in molecular pathology such as FISH, direct sequencing, pyrosequencing, and allele-specific PCR will be replaced by massive parallel sequencing that will not be considered next generation, but rather, will be considered to be current generation sequencing...
November 29, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27896036/detecting-sedimentation-impacts-to-coral-reefs-resulting-from-dredging-the-port-of-miami-florida-usa
#6
Margaret W Miller, Jocelyn Karazsia, Carolyn E Groves, Sean Griffin, Tom Moore, Pace Wilber, Kurtis Gregg
The federal channel at Port of Miami, Florida, USA, was dredged between late 2013 and early 2015 to widen and deepen the channel. Due to the limited spatial extent of impact-assessment monitoring associated with the project, the extent of the dredging impacts on surrounding coral reefs has not been well quantified. Previously published remote sensing analyses, as well as agency and anecdotal reports suggest the most severe and largest area of sedimentation occurred on a coral reef feature referred to as the Inner Reef, particularly in the sector north of the channel...
2016: PeerJ
https://www.readbyqxmd.com/read/27876391/critical-evaluation-of-monitoring-strategy-for-the-multi-residue-determination-of-90-chiral-and-achiral-micropollutants-in-effluent-wastewater
#7
Bruce Petrie, Kathryn Proctor, Jane Youdan, Ruth Barden, Barbara Kasprzyk-Hordern
It is essential to monitor the release of organic micropollutants from wastewater treatment plants (WWTPs) for developing environmental risk assessment and assessing compliance with legislative regulation. In this study the impact of sampling strategy on the quantitative determination of micropollutants in effluent wastewater was investigated. An extended list of 90 chiral and achiral micropollutants representing a broad range of biological and physico-chemical properties were studied simultaneously for the first time...
November 19, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27875023/myxochelin-inspired-5-lipoxygenase-inhibitors-synthesis-and-biological-evaluation
#8
Sebastian Schieferdecker, Stefanie König, Simona Pace, Oliver Werz, Markus Nett
A total of 48 analogues of the natural product myxochelin A were prepared and evaluated for their inhibitory effects on human 5-lipoxygenase in both cell-free and cell-based assays. Structure-activity relationship analysis revealed that the secondary alcohol function and only chiral center of myxochelin A is not required for the biological activity. By expanding the diaminoalkane linker of the two aromatic residues it was possible to generate a myxochelin derivative with superior activity against 5-lipoxygenase in intact cells...
November 22, 2016: ChemMedChem
https://www.readbyqxmd.com/read/27871856/will-cardiac-optogenetics-find-the-way-through-the-obscure-angles-of-heart-physiology
#9
Nicola Pianca, Tania Zaglia, Marco Mongillo
Optogenetics is a technique exploded in the last 10 years, which revolutionized several areas of biological research. The brightest side of this technology is the use of light to modulate non-invasively, with high spatial resolution and millisecond time scale, excitable cells genetically modified to express light-sensitive microbial ion channels (opsins). Neuroscience has first benefited from such fascinating strategy, in intact organisms. By shining light to specific neuronal subpopulations, optogenetics allowed unearth the mechanisms involved in cell-to-cell communication within the context of intact organs, such as the brain, formed by complex neuronal circuits...
November 18, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27870114/the-molecular-genetics-of-autosomal-recessive-nonsyndromic-intellectual-disability-a-mutational-continuum-and-future-recommendations
#10
REVIEW
Muzammil Ahmad Khan, Saadullah Khan, Christian Windpassinger, Muhammad Badar, Zafar Nawaz, Ramzi M Mohammad
Intellectual disability (ID) is a clinical manifestation of the central nervous system without any major dysmorphologies of the brain. Biologically it affects learning capabilities, memory, and cognitive functioning. The basic defining features of ID are characterized by IQ<70, age of onset before 18 years, and impairment of at least two of the adaptive skills. Clinically it is classified in a syndromic (with additional abnormalities) and a nonsyndromic form (with only cognitive impairment). The study of nonsyndromic intellectual disability (NSID) can best explain the pathophysiology of cognition, intelligence and memory...
November 2016: Annals of Human Genetics
https://www.readbyqxmd.com/read/27846849/untargeted-metabolomics-unravels-functionalities-of-phosphorylation-sites-in-saccharomyces-cerevisiae
#11
Zrinka Raguz Nakic, Gerhard Seisenbacher, Francesc Posas, Uwe Sauer
BACKGROUND: Coordinated through a complex network of kinases and phosphatases, protein phosphorylation regulates essentially all cellular processes in eukaryotes. Recent advances in proteomics enable detection of thousands of phosphorylation sites (phosphosites) in single experiments. However, functionality of the vast majority of these sites remains unclear and we lack suitable approaches to evaluate functional relevance at a pace that matches their detection. RESULTS: Here, we assess functionality of 26 phosphosites by introducing phosphodeletion and phosphomimic mutations in 25 metabolic enzymes and regulators from the TOR and HOG signaling pathway in Saccharomyces cerevisiae by phenotypic analysis and untargeted metabolomics...
November 15, 2016: BMC Systems Biology
https://www.readbyqxmd.com/read/27836594/photoelectrochemical-enzymatic-biosensors
#12
REVIEW
Wei-Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Enzymatic biosensors have been valuable bioanalytical devices for analysis of diverse targets in disease diagnosis, biological and biomedical research, etc. Photoelectrochemical (PEC) bioanalysis is a recently emerged method that promptly becoming a subject of new research interests due to its attractive potential for future bioanalysis with high sensitivity and specificity. PEC enzymatic biosensors integrate the inherent sensitivities of PEC bioanalysis and the selectivity of enzymes and thus share their both advantages...
November 7, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27822170/proteomics-in-india-the-clinical-aspect
#13
REVIEW
Somaditya Mukherjee, Arun Bandyopadhyay
Proteomics has emerged as a highly promising bioanalytical technique in various aspects of applied biological research. In Indian academia, proteomics research has grown remarkably over the last decade. It is being extensively used for both basic as well as translation research in the areas of infectious and immune disorders, reproductive disorders, cardiovascular diseases, diabetes, eye disorders, human cancers and hematological disorders. Recently, some seminal works on clinical proteomics have been reported from several laboratories across India...
2016: Clinical Proteomics
https://www.readbyqxmd.com/read/27812435/loitering-of-the-retreating-sea-ice-edge-in-the-arctic-seas
#14
Michael Steele, Wendy Ermold
Each year, the arctic sea ice edge retreats from its winter maximum extent through the Seasonal Ice Zone (SIZ) to its summer minimum extent. On some days, this retreat happens at a rapid pace, while on other days, parts of the pan-arctic ice edge hardly move for periods of days up to 1.5 weeks. We term this stationary behavior "ice edge loitering," and identify areas that are more prone to loitering than others. Generally, about 20-25% of the SIZ area experiences loitering, most often only one time at any one location during the retreat season, but sometimes two or more times...
December 2015: Journal of Geophysical Research. Oceans
https://www.readbyqxmd.com/read/27810480/evaluation-of-relational-and-nosql-database-architectures-to-manage-genomic-annotations
#15
Wade L Schulz, Brent G Nelson, Donn K Felker, Thomas J S Durant, Richard Torres
While the adoption of next generation sequencing has rapidly expanded, the informatics infrastructure used to manage the data generated by this technology has not kept pace. Historically, relational databases have provided much of the framework for data storage and retrieval. Newer technologies based on NoSQL architectures may provide significant advantages in storage and query efficiency, thereby reducing the cost of data management. But their relative advantage when applied to biomedical data sets, such as genetic data, has not been characterized...
December 2016: Journal of Biomedical Informatics
https://www.readbyqxmd.com/read/27803741/the-biological-complexity-of-colorectal-cancer-insights-into-biomarkers-for-early-detection-and-personalized-care
#16
REVIEW
Marina De Rosa, Daniela Rega, Valeria Costabile, Francesca Duraturo, Antonello Niglio, Paola Izzo, Ugo Pace, Paolo Delrio
Colorectal cancer has been ranked the third and second most prevalent of all cancers in men and women, respectively, and it represents the fourth most common cause of cancer deaths. In 2012, there were 1.4 million estimated cases of colorectal cancer worldwide, and 700,000 estimated deaths, which implies significant impact on public health, especially in economically-developed countries. In recent years, there has been an increase in the number of tumors, although this has been accompanied by decreased mortality, due to more appropriate and available information, earlier diagnosis, and improvements in treatment...
November 2016: Therapeutic Advances in Gastroenterology
https://www.readbyqxmd.com/read/27789703/prokaryotic-virus-orthologous-groups-pvogs-a-resource-for-comparative-genomics-and-protein-family-annotation
#17
Ana Laura Grazziotin, Eugene V Koonin, David M Kristensen
Viruses are the most abundant and diverse biological entities on earth, and while most of this diversity remains completely unexplored, advances in genome sequencing have provided unprecedented glimpses into the virosphere. The Prokaryotic Virus Orthologous Groups (pVOGs, formerly called Phage Orthologous Groups, POGs) resource has aided in this task over the past decade by using automated methods to keep pace with the rapid increase in genomic data. The uses of pVOGs include functional annotation of viral proteins, identification of genes and viruses in uncharacterized DNA samples, phylogenetic analysis, large-scale comparative genomics projects, and more...
October 26, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27778110/surface-proteome-mining-for-identification-of-potential-vaccine-candidates-against-campylobacter-jejuni-an-in-silico-approach
#18
Kusum Mehla, Jayashree Ramana
Campylobacter jejuni remains a major cause of human gastroenteritis with estimated annual incidence rate of 450 million infections worldwide. C. jejuni is a major burden to public health in both socioeconomically developing and industrialized nations. Virulence determinants involved in C. jejuni pathogenesis are multifactorial in nature and not yet fully understood. Despite the completion of the first C. jejuni genome project in 2000, there are currently no vaccines in the market against this pathogen. Traditional vaccinology approach is an arduous and time extensive task...
October 24, 2016: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/27760370/stem-cell-therapy-clinical-research-a-regulatory-conundrum-for-academia
#19
Anjali Nagpal, Chris Juttner, Monica Anne Hamilton-Bruce, Paul Rolan, Simon A Koblar
The encouraging pace of discovery and development in the field of regenerative medicine holds tremendous potential for bringing therapies to the clinic that may offer meaningful benefit to patients, particularly in diseases with no or suboptimal therapeutic options. Academic researchers will continue to play a critical role in developing concepts and therapies, thus determining whether regenerative medicine will be able to live up to this potential that clearly excites clinicians, researchers and patients alike...
October 17, 2016: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/27749002/novel-factor-viii-variants-with-a-modified-furin-cleavage-site-improve-the-efficacy-of-gene-therapy-for-hemophilia-a
#20
G N Nguyen, L A George, J I Siner, R J Davidson, C B Zander, X L Zheng, V R Arruda, R M Camire, D E Sabatino
BACKGROUND: The major challenge for developing gene-based therapies for hemophilia A is that human factor VIII (hFVIII) has intrinsic properties that result in inefficient biosynthesis. During intracellular processing, hFVIII is predominantly cleaved at a Paired basic Amino acid Cleaving Enzyme (PACE) or furin cleavage site to yield a heterodimer that is the major form of secreted protein. Previous studies with B-domain deleted (BDD) canine FVIII and hFVIII-R1645H, both differing from hFVIII by a single amino acid at this site, suggested that these proteins are secreted mainly in a single polypeptide chain (SC) form and exhibit enhanced function...
October 17, 2016: Journal of Thrombosis and Haemostasis: JTH
keyword
keyword
9141
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"