keyword
MENU ▼
Read by QxMD icon Read
search

Biology pacing

keyword
https://www.readbyqxmd.com/read/28435300/the-identification-of-protein-domains-that-mediate-functional-interactions-between-rab-gtpases-and-rabgaps-using-3d-protein-modeling
#1
Jeremiah J Davie, Silviu L Faitar
Currently, time-consuming serial in vitro experimentation involving immunocytochemistry or radiolabeled materials is required to identify which of the numerous Rab-GTPases (Rab) and Rab-GTPase activating proteins (RabGAP) are capable of functional interactions. These interactions are essential for numerous cellular functions, and in silico methods of reducing in vitro trial and error would accelerate the pace of research in cell biology. We have utilized a combination of three-dimensional protein modeling and protein bioinformatics to identify domains present in Rab proteins that are predictive of their functional interaction with a specific RabGAP...
2017: Advances and Applications in Bioinformatics and Chemistry: AABC
https://www.readbyqxmd.com/read/28435092/-terpinen-4-ol-changes-intracellular-ca-2-handling-and-induces-pacing-disturbance-in-rat-hearts
#2
Antonio Nei Santana Gondim, Aline Lara, Artur Santos-Miranda, Danilo Roman-Campos, Sandra Lauton-Santos, José Evaldo Rodrigues Menezes-Filho, Carla Maria Lins de Vasconcelos, Eduardo Antonio Conde-Garcia, Silvia Guatimosim, Jader S Cruz
(-)-Terpinen-4-ol is a naturally occurring plant monoterpene and has been shown to have a plethora of biological activities. The objective of this study was to investigate the effects of (-)-terpinen-4-ol on the rat heart, a key player in the control and maintenance of arterial blood pressure. The effects of (-)-terpinen-4-ol on the rat heart were investigated using isolated left atrium isometric force measurements, in vivo electrocardiogram (ECG) recordings, patch clamp technique, and confocal microscopy. It was observed that (-)-terpinen-4-ol reduced contraction force in an isolated left atrium at millimolar concentrations...
April 20, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28434256/a-bioinformatics-roadmap-for-the-human-vaccines-project
#3
Richard H Scheuermann, Robert S Sinkovits, Theodore Schenkelberg, Wayne C Koff
Biomedical research has become a data intensive science in which high throughput experimentation is producing comprehensive data about biological systems at an ever-increasing pace. The Human Vaccines Project is a new public-private partnership, with the goal of accelerating development of improved vaccines and immunotherapies for global infectious diseases and cancers by decoding the human immune system. To achieve its mission, the Project is developing a Bioinformatics Hub as an open-source, multidisciplinary effort with the overarching goal of providing an enabling infrastructure to support the data processing, analysis and knowledge extraction procedures required to translate high throughput, high complexity human immunology research data into biomedical knowledge, to determine the core principles driving specific and durable protective immune responses...
April 24, 2017: Expert Review of Vaccines
https://www.readbyqxmd.com/read/28428391/the-interaction-of-human-population-food-production-and-biodiversity-protection
#4
REVIEW
Eileen Crist, Camilo Mora, Robert Engelman
Research suggests that the scale of human population and the current pace of its growth contribute substantially to the loss of biological diversity. Although technological change and unequal consumption inextricably mingle with demographic impacts on the environment, the needs of all human beings-especially for food-imply that projected population growth will undermine protection of the natural world. Numerous solutions have been proposed to boost food production while protecting biodiversity, but alone these proposals are unlikely to staunch biodiversity loss...
April 21, 2017: Science
https://www.readbyqxmd.com/read/28422050/k-mer-content-correlation-and-position-analysis-of-genome-dna-sequences-for-the-identification-of-function-and-evolutionary-features
#5
Aaron Sievers, Katharina Bosiek, Marc Bisch, Chris Dreessen, Jascha Riedel, Patrick Froß, Michael Hausmann, Georg Hildenbrand
In genome analysis, k-mer-based comparison methods have become standard tools. However, even though they are able to deliver reliable results, other algorithms seem to work better in some cases. To improve k-mer-based DNA sequence analysis and comparison, we successfully checked whether adding positional resolution is beneficial for finding and/or comparing interesting organizational structures. A simple but efficient algorithm for extracting and saving local k-mer spectra (frequency distribution of k-mers) was developed and used...
April 19, 2017: Genes
https://www.readbyqxmd.com/read/28419534/endocrine-imbalance-associated-with-proteome-changes-in-diabetes
#6
Amr A Sayed, Ahmed M Alhawary, Aboalela Farag, Dina R Johar, Larry H Bernstein
The dynamics of cellular metabolism involves rapid interactions between proteins and nucleotides, proteins and proteins, proteins and mRNA, the action of miRNA, and signaling. These also involve the interactions with respect to the sulfur bond, oxygen radicals that initiate a change in conformation and a chain of events. We review a development in molecular medicine that is a very promising work in progress. We also review the current and future research methods involving mitochondria. Long-term effects of diabetes include glycation of proteins, e...
April 17, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28401731/impact-of-early-personal-history-characteristics-on-the-pace-of-aging-implications-for-clinical-trials-of-therapies-to-slow-aging-and-extend-healthspan
#7
Daniel W Belsky, Avshalom Caspi, Harvey J Cohen, William E Kraus, Sandhya Ramrakha, Richie Poulton, Terrie E Moffitt
Therapies to extend healthspan are poised to move from laboratory animal models to human clinical trials. Translation from mouse to human will entail challenges, among them the multifactorial heterogeneity of human aging. To inform clinical trials about this heterogeneity, we report how humans' pace of biological aging relates to personal-history characteristics. Because geroprotective therapies must be delivered by midlife to prevent age-related disease onset, we studied young-adult members of the Dunedin Study 1972-73 birth cohort (n = 954)...
April 12, 2017: Aging Cell
https://www.readbyqxmd.com/read/28380383/using-dna-methylation-profiling-to-evaluate-biological-age-and-longevity-interventions
#8
Daniel A Petkovich, Dmitriy I Podolskiy, Alexei V Lobanov, Sang-Goo Lee, Richard A Miller, Vadim N Gladyshev
The DNA methylation levels of certain CpG sites are thought to reflect the pace of human aging. Here, we developed a robust predictor of mouse biological age based on 90 CpG sites derived from partial blood DNA methylation profiles. The resulting clock correctly determines the age of mouse cohorts, detects the longevity effects of calorie restriction and gene knockouts, and reports rejuvenation of fibroblast-derived iPSCs. The data show that mammalian DNA methylomes are characterized by CpG sites that may represent the organism's biological age...
April 4, 2017: Cell Metabolism
https://www.readbyqxmd.com/read/28371485/target-deconvolution-efforts-on-wnt-pathway-screen-reveal-dual-modulation-of-oxidative-phosphorylation-and-serca2
#9
Matias Casas-Selves, Andrew Zhang, James E Dowling, Stefan Hallen, Aarti Kawatkar, Nicholas J Pace, Christopher Denz, Timothy Pontz, Farzin Garahdaghi, Qing Cao, Alan Sabirsh, Kumar Thakur, Nichole O'Connell, Jun Hu, Ivan Cornella-Taracido, Eranthie Weerapana, Michael Zinda, Robert A Goodnow
Wnt signaling is critical for development, cell proliferation and differentiation, and mutations in this pathway resulting in constitutive signaling have been implicated in various cancers. A pathway screen using a Wnt-dependent reporter identified a chemical series based on a 1,2,3-thiadiazole-5-carboxamide (TDZ) core with sub-M potency. We report here a comprehensive mechanism of action deconvolution study towards identifying the efficacy target(s) and biological implication of this chemical series involving bottom-up quantitative chemoproteomics, cell biology, and biochemical methods...
March 29, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28367360/tissue-engineered-larynx-future-applications-in-laryngeal-cancer
#10
REVIEW
Nick J I Hamilton, Martin A Birchall
PURPOSE OF REVIEW: This article reviews the latest developments in tissue engineering for the larynx with a specific focus on the treatment of laryngeal cancer. RECENT FINDINGS: Challenges in tissue engineering a total larynx can be divided into scaffold design, methods of re-mucosalization, and how to restore laryngeal function. The literature described a range of methods to deliver a laryngeal scaffold including examples of synthetic, biomimetic, and biological scaffolds...
2017: Current Otorhinolaryngology Reports
https://www.readbyqxmd.com/read/28351529/analysis-of-the-regulation-of-surfactant-phosphatidylcholine-metabolism-using-stable-isotopes
#11
REVIEW
Joost Brandsma, Anthony D Postle
The pathways and mechanisms that regulate pulmonary surfactant synthesis, processing, secretion and catabolism have been extensively characterised using classical biochemical and analytical approaches. These have constructed a model, largely in experimental animals, for surfactant phospholipid metabolism in the alveolar epithelial cell whereby phospholipid synthesised on the endoplasmic reticulum is selectively transported to lamellar body storage vesicles, where it is subsequently processed before secretion into the alveolus...
March 25, 2017: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/28334154/ethical-issues-of-crispr-technology-and-gene-editing-through-the-lens-of-solidarity
#12
John J Mulvihill, Benjamin Capps, Yann Joly, Tamra Lysaght, Hub A E Zwart, Ruth Chadwick
Background: The avalanche of commentaries on CRISPR-Cas9 technology, a bacterial immune system modified to recognize any short DNA sequence, cut it out, and insert a new one, has rekindled hopes for gene therapy and other applications and raised criticisms of engineering genes in future generations. Sources of data: This discussion draws on articles that emphasize ethics, identified partly through PubMed and Google, 2014-2016. Areas of agreement: CRISPR-Cas9 has taken the pace and prospects for genetic discovery and applications to a high level, stoking anticipation for somatic gene engineering to help patients...
February 23, 2017: British Medical Bulletin
https://www.readbyqxmd.com/read/28301747/single-cell-transcriptional-analysis
#13
Angela R Wu, Jianbin Wang, Aaron M Streets, Yanyi Huang
Despite being a relatively recent technological development, single-cell transcriptional analysis through high-throughput sequencing has already been used in hundreds of fruitful studies to make exciting new biological discoveries that would otherwise be challenging or even impossible. Consequently, this has fueled a virtuous cycle of even greater interest in the field and compelled development of further improved technical methodologies and approaches. Thanks to the combined efforts of the research community, including the fields of biochemistry and molecular biology, technology and instrumentation, data science, computational biology, and bioinformatics, the single-cell RNA-sequencing field is advancing at a pace that is both astounding and unprecedented...
March 16, 2017: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/28293165/-pyr-1-apelin-13-1-12-is-a-biologically-active-ace2-metabolite-of-the-endogenous-cardiovascular-peptide-pyr-1-apelin-13
#14
Peiran Yang, Rhoda E Kuc, Aimée L Brame, Alex Dyson, Mervyn Singer, Robert C Glen, Joseph Cheriyan, Ian B Wilkinson, Anthony P Davenport, Janet J Maguire
Aims: Apelin is a predicted substrate for ACE2, a novel therapeutic target. Our aim was to demonstrate the endogenous presence of the putative ACE2 product [Pyr(1)]apelin-13(1-12) in human cardiovascular tissues and to confirm it retains significant biological activity for the apelin receptor in vitro and in vivo. The minimum active apelin fragment was also investigated. Methods and Results: [Pyr(1)]apelin-13 incubated with recombinant human ACE2 resulted in de novo generation of [Pyr(1)]apelin-13(1-12) identified by mass spectrometry...
2017: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/28277904/genome-wide-functional-analysis-in-candida-albicans
#15
Thabiso E Motaung, Ruan Ells, Carolina H Pohl, Jacobus Albertyn, Toi J Tsilo
Candida albicans is an important etiological agent of superficial and life-threatening infections in individuals with compromised immune systems. To date, we know of several overlapping genetic networks that govern virulence attributes in this fungal pathogen. Classical use of deletion mutants has led to the discovery of numerous virulence factors over the years, and genome-wide functional analysis has propelled gene discovery at an even faster pace. Indeed, a number of recent studies using large-scale genetic screens followed by genome-wide functional analysis has allowed for the unbiased discovery of many new genes involved in C...
February 8, 2017: Virulence
https://www.readbyqxmd.com/read/28277544/long-noncoding-rna-blacat1-indicates-a-poor-prognosis-of-colorectal-cancer-and-affects-cell-proliferation-by-epigenetically-silencing-of-p15
#16
Jun Su, Erbao Zhang, Liang Han, Dandan Yin, Zhili Liu, Xuezhi He, Yuhong Zhang, Feng Lin, Qingfeng Lin, Peiyao Mao, Weidong Mao, Dong Shen
Recently, a novel class of transcripts, long noncoding RNAs (lncRNAs), is being identified at a rapid pace. These RNAs have critical roles in diverse biological processes, including tumorigenesis. One of them, BLACAT1, a cancer-associated long noncoding RNA, exerts regulatory functions in various biological processes in cancer cells, however, the role of BLACAT1 in colon cancer remains unclear. Our experiments showed that increased BLACAT1 was an independent unfavorable prognostic indicator for colorectal cancer, and revealed that BLACAT1 knockdown significantly repressed proliferation, both in vitro and in vivo...
March 9, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28270811/animal-models-of-leptospirosis-of-mice-and-hamsters
#17
REVIEW
Maria Gomes-Solecki, Ignacio Santecchia, Catherine Werts
Pathogenic Leptospira sp. are spirochetal bacteria responsible for leptospirosis, an emerging worldwide zoonosis. These spirochetes are very successful pathogens that infect a wide range of hosts such as fish, reptiles, birds, marsupials, and mammals. Transmission occurs when chronically infected animals excrete live bacteria in their urine, contaminating the environment. Leptospira sp. enter their hosts through damaged skin and mucosa. Chronically infected rats and mice are asymptomatic and are considered as important reservoirs of the disease...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28263774/biologic-response-modifiers-indications-implications-and-insights
#18
Benjamin P Davis, Zuhair K Ballas
The field of biologic immune-modulators is currently mushrooming at a dizzying pace. While most of these biologics are tested and approved for one or a few indications, their un-anticipated side effects and their off-label use have contributed significantly to our understanding of basic immune mechanisms, of the involvement of cytokines in several apparently non-immunologic diseases, and of the importance of compartmentalized immune responses. In this review, we attempt to give a bird's-eye view of the major biologics and to highlight insights and implications derived from their secondary effects and adverse reactions...
March 2, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28261549/bioengineering-hearts-simple-yet-complex
#19
REVIEW
Doris A Taylor, Rohan B Parikh, Luiz C Sampaio
PURPOSE OF REVIEW: In this review, we focus on the multiple advancements in the field of cardiovascular regenerative medicine and the state-of-the art of building a heart. An organ is comprised of cells, but cells alone do not comprise an organ. We summarize the components needed, the hurdles, and likely translational steps defining the opportunities for discovery. RECENT FINDINGS: The therapies being developed in regenerative medicine aim not only to repair, but also to regenerate or replace ailing tissues and organs...
2017: Current Stem Cell Reports
https://www.readbyqxmd.com/read/28256494/total-knee-replacement-in-a-resource-constrained-environment-a-preliminary-report
#20
U E Anyaehie, G O Eyichukwu, C U Nwadinigwe
INTRODUCTION: Total knee replacement surgery is relatively new in Nigeria and available in few centres only. It has been evolving at a slow pace because of the lack of facilities, structures and adequate surgical expertise alongside patient ignorance and poverty. OBJECTIVE: The aim of this article is to review the cases done in a resource constrained institution in Nigeria and report the short term outcomes. METHODOLOGY: All the patients that were booked for Total knee replacement, using same prosthesis over a 40 month period, were recruited into the study...
March 2017: Nigerian Journal of Clinical Practice
keyword
keyword
9141
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"