keyword
MENU ▼
Read by QxMD icon Read
search

Igf2 AND insulin

keyword
https://www.readbyqxmd.com/read/29626476/long-non-coding-rna-thor-promotes-human-osteosarcoma-cell-growth-in-vitro-and-in-vivo
#1
Wangzhen Chen, Meikai Chen, Yifan Xu, Xuerong Chen, Ping Zhou, Xiaofeng Zhao, Fei Pang, Wenqing Liang
Long non-coding RNA (LncRNA) dysregulation is associated with human osteosarcoma (OS) cell progression. Recent studies have characterized a novel but ultra-conserved LncRNA THOR ("Lnc-THOR") as a cancer-specific LncRNA, mediating cell growth. In the current study, we show that Lnc-THOR is expressed in established and primary human OS cells. It is also detected in human OS tissues, but not in the surrounding normal bone tissues. siRNA-induced knockdown or CRSIPR/Cas9-mediated knockout Lnc-THOR significantly inhibited human OS cell survival and proliferation...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29615365/detection-of-insulin-like-growth-factor-receptor-1-in-the-human-cremaster-muscle-and-its-role-in-the-etiology-of-the-undescended-testis
#2
Mustafa Yaşar Özdamar, Sevinç Şahin, Kürşad Zengin, Selda Seçkin, Mesut Gürdal
PURPOSE: Previous studies of undescended testis (UT) has focused on insulin-like hormone 3 (INSL3), the genitofemoral nerve, and androgens in the testicular descent. Leydig cells, which are under the control of insulin-like growth factor-1 (IGF1), produce both androgens and INSL3. We aimed to investigate whether insulin-like growth factor receptor-1(IGFR1) exists in the cremaster muscle (CM) complex and is associated with normally descended testis as well as UT cases in humans. METHODS: We studied 30 CM from 15 patients who comprised the UT group (UTG), and 15 patients with unilateral testicular torsion (Control group; CG)...
March 31, 2018: Asian Journal of Surgery
https://www.readbyqxmd.com/read/29611103/evidence-for-compromised-insulin-signaling-and-neuronal-vulnerability-in-experimental-model-of-sporadic-alzheimer-s-disease
#3
Smriti Gupta, Kamalendra Yadav, Shrikant S Mantri, Nitin K Singhal, Subramaniam Ganesh, Rajat Sandhir
Evidence from animal studies categorizes sporadic Alzheimer's disease (sAD) as a metabolic syndrome with accompanying cognitive deficits. Given that glial cells act as "silent partners" to neurons by providing trophic support and defense, the present study investigated the role of glia in sAD pathology. A streptozotocin (STZ)-induced glial-neuronal co-culture model of sAD was used to study the metabolic status of the two cell types. Real time RT-PCR and Western blotting results indicated that amyloid precursor protein (APP) and β-secretase (BACE1) were highly expressed in co-cultured neurons than in monocultures...
April 3, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29603098/dna-methylation-of-genes-regulating-appetite-and-prediction-of-weight-loss-after-bariatric-surgery-in-obese-individuals
#4
F Coppedè, M Seghieri, A Stoccoro, E Santini, L Giannini, C Rossi, L Migliore, A Solini
PURPOSE: Epigenetic traits are influenced by clinical variables; interaction between DNA methylation (DNAmeth) and bariatric surgery-induced weight loss has been scarcely explored. We investigated whether DNAmeth of genes encoding for molecules/hormones regulating appetite, food intake or obesity could predict successful weight outcome following Roux-en-Y gastric bypass (RYGB). METHODS: Forty-five obese individuals with no known comorbidities were stratified accordingly to weight decrease one-year after RYGB (excess weight loss, EWL ≥ 50%: good responders, GR; EWL < 50%: worse responders, WR)...
March 30, 2018: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/29602297/similarity-and-variation-in-the-insulin-like-growth-factor-2-h19-locus-in-primates
#5
Peter Rotwein
Insulin-like growth factor 2 (IGF2), a small, secreted protein, is critical for fetal and prenatal growth in humans and other mammals. The IGF2 gene and its mouse homologue comprise part of a conserved linkage group that is regulated by parental imprinting, with IGF2/Igf2 being expressed from the paternal chromosome, and the adjacent H19 gene from the maternal chromosome. By using information extracted from public genomic and gene expression databases, this locus now has been analyzed in nine nonhuman primate species representing over 60 million years of evolutionary divergence from a common progenitor...
March 30, 2018: Physiological Genomics
https://www.readbyqxmd.com/read/29562959/microrna-615-3p-inhibits-the-tumor-growth-and-metastasis-of-nsclc-via-inhibiting-igf2
#6
Jiangtao Liu, Yanli Jia, Lijuan Jia, Tingting Li, Lei Yang, Gongwen Zhang
MicroRNAs are essential regulators of cancer-associated gene at the post-transcriptional level and their expression isaltered in cancer tissues. Herein, we sought to identify the regulation of miR-615-3p in NSCLC progression and its mechanism. MiR-615-3p expression was significantly down-expression in NSCLC tissue than that in control normal tissue. Exogenous over-expression of miR-615-3p inhibited the growth and metastasis of NSCLC cells. In addition, the in vivo mouse xenograft model showed that over-expression of miR615-3p inhibited NSCLC growth and lung metastasis whereas down-expression of miR-615-3p caused an opposite outcome...
March 21, 2018: Oncology Research
https://www.readbyqxmd.com/read/29558395/yak-igf2-promotes-fibroblast-proliferation-via-suppression-of-igf1r-and-pi3kcg-expression
#7
Quanwei Zhang, Qi Wang, Jishang Gong, Jiaxing Du, Yong Zhang, Xingxu Zhao
Insulin-like growth factor 2 (IGF2) recapitulates many of the activities of insulin and promotes differentiation of myoblasts and osteoblasts, which likely contribute to genetic variations of growth potential. However, little is known about the functions and signaling properties of IGF2 variants in yaks. The over-expression vector and knockdown sequence of yak IGF2 were transfected into yak fibroblasts, and the effects were detected by a series of assays. IGF2 expression in yak muscle tissues was significantly lower than that of other tissues...
March 20, 2018: Genes
https://www.readbyqxmd.com/read/29551271/dna-conformation-induces-adaptable-binding-by-tandem-zinc-finger-proteins
#8
Anamika Patel, Peng Yang, Matthew Tinkham, Mihika Pradhan, Ming-An Sun, Yixuan Wang, Don Hoang, Gernot Wolf, John R Horton, Xing Zhang, Todd Macfarlan, Xiaodong Cheng
Tandem zinc finger (ZF) proteins are the largest and most rapidly diverging family of DNA-binding transcription regulators in mammals. ZFP568 represses a transcript of placental-specific insulin like growth factor 2 (Igf2-P0) in mice. ZFP568 binds a 24-base pair sequence-specific element upstream of Igf2-P0 via the eleven-ZF array. Both DNA and protein conformations deviate from the conventional one finger-three bases recognition, with individual ZFs contacting 2, 3, or 4 bases and recognizing thymine on the opposite strand...
March 13, 2018: Cell
https://www.readbyqxmd.com/read/29542837/testicular-development-in-male-lambs-prenatally-exposed-to-a-high-starch-diet
#9
Francesca Mossa, Daniela Bebbere, Antonello Ledda, Giovanni P Burrai, Imane Chebli, Elisabetta Antuofermo, Sergio Ledda, Antonello Cannas, Francesco Fancello, Alberto S Atzori
Maternal nutrition during critical gestation periods impacts on offspring in later life; effects of high-starch maternal diet on testicular development in lambs were addressed. Dairy ewes were fed diets providing either 27% (Starch, S) or 11% (Fiber, F) of starch from mating to lambing (∼147 days; S147, F147) or for the last 75 days of gestation (S75, F75). Testes of single male lambs were measured and then sampled for histological and gene expression analyses at selected ages. Testicular dimensions and weight were similar among groups, but the total area of seminiferous tubules increased with age and tended to be higher (P = 0...
March 15, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29514882/genomic-imprinting-growth-and-maternal-fetal-interactions
#10
REVIEW
Féaron C Cassidy, Marika Charalambous
In the 1980s, mouse nuclear transplantation experiments revealed that both male and female parental genomes are required for successful development to term ( McGrath and Solter, 1983; Surani and Barton, 1983). This non-equivalence of parental genomes is because imprinted genes are predominantly expressed from only one parental chromosome. Uniparental inheritance of these genomic regions causes paediatric growth disorders such as Beckwith-Wiedemann and Silver-Russell syndromes (reviewed in Peters, 2014). More than 100 imprinted genes have now been discovered and the functions of many of these genes have been assessed in murine models...
March 7, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29490235/premature-hippocampus-dependent-memory-decline-in-middle-aged-females-of-a-genetic-rat-model-of-depression
#11
Patrick H Lim, Stephanie L Wert, Elif Tunc-Ozcan, Robert Marr, Adriana Ferreira, Eva E Redei
Aging and major depressive disorder are risk factors for dementia, including Alzheimer's Disease (AD), but the mechanism(s) linking depression and dementia are not known. Both AD and depression show greater prevalence in women. We began to investigate this connection using females of the genetic model of depression, the inbred Wistar Kyoto More Immobile (WMI) rat. These rats consistently display depression-like behavior compared to the genetically close control, the Wistar Kyoto Less Immobile (WLI) strain. Hippocampus-dependent contextual fear memory did not differ between young WLI and WMI females, but, by middle-age, female WMIs showed memory deficits compared to same age WLIs...
February 25, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29470838/first-line-cetuximab-monotherapy-in-kras-nras-braf-mutation-negative-colorectal-cancer-patients
#12
Vladimir M Moiseyenko, Fedor V Moiseyenko, Grigoriy A Yanus, Ekatherina Sh Kuligina, Anna P Sokolenko, Ilya V Bizin, Alexey A Kudriavtsev, Svetlana N Aleksakhina, Nikita M Volkov, Vyacheslav A Chubenko, Kseniya S Kozyreva, Mikhail M Kramchaninov, Alexandr S Zhuravlev, Kseniya V Shelekhova, Denis V Pashkov, Alexandr O Ivantsov, Aigul R Venina, Tatyana N Sokolova, Elena V Preobrazhenskaya, Natalia V Mitiushkina, Alexandr V Togo, Aglaya G Iyevleva, Evgeny N Imyanitov
BACKGROUND: Colorectal carcinomas (CRCs) are sensitive to treatment by anti-epidermal growth factor receptor (EGFR) antibodies only if they do not carry activating mutations in down-stream EGFR targets (KRAS/NRAS/BRAF). Most clinical trials for chemo-naive CRC patients involved combination of targeted agents and chemotherapy, while single-agent cetuximab or panitumumab studies included either heavily pretreated patients or subjects who were not selected on the basis of molecular tests...
February 22, 2018: Clinical Drug Investigation
https://www.readbyqxmd.com/read/29455465/insulin-like-growth-factor-2-a-poor-prognostic-biomarker-linked-to-racial-disparity-in-women-with-uterine-carcinosarcoma
#13
Anne R Van Arsdale, Rebecca C Arend, Maria J Cossio, Britt K Erickson, Yanhua Wang, David W Doo, Charles A Leath, Gary L Goldberg, Gloria S Huang
The objective of this study was to investigate the relationship of insulin-like growth factor 2 (IGF2) expression and survival in women with uterine carcinosarcoma (UCS). Insulin-like growth factor 2 protein expression was determined by immunohistochemical staining of tumor tissues from 103 patients with UCS. The H-score (product of staining intensity and percentage positive cells) was quantified for the epithelial cytoplasmic (EC), epithelial nuclear (EN), and malignant stromal compartments. Multivariable Cox proportional hazard regression models were used to examine the relationship of IGF2 levels with progression-free survival (PFS) and overall survival (OS)...
February 18, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29425714/leucyl-trna-synthetase-is-required-for-the-myogenic-differentiation-of-c2c12-myoblasts-but-not-for-hypertrophy-or-metabolic-alteration-of-myotubes
#14
Yoriko Sato, Yusuke Sato, Reiko Suzuki, Kodwo Obeng, Fumiaki Yoshizawa
Mammalian target of rapamycin (mTOR) signaling controls skeletal muscle cell differentiation, growth, and metabolism by sensing the intracellular energy status and nutrients. Recently, leucyl-tRNA synthetase (Lars) was identified as an intracellular sensor of leucine involved in the activation of mTOR signaling. However, there is still no evidence for the activation of mTOR signaling by Lars and its physiological roles in skeletal muscle cells. In this study, we determined the potential roles of Lars for the activation of mTOR signaling, skeletal muscle cell differentiation, hypertrophy, and metabolism using small interfering (si)-RNA knockdown...
February 6, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29414792/the-complex-genetics-of-human-insulin-like-growth-factor-2-are-not-reflected-in-public-databases
#15
Peter Rotwein
Recent advances in genetics present unique opportunities for enhancing knowledge about human physiology and disease susceptibility. Understanding this information at the individual gene level is challenging, and requires extracting, collating and interpreting data from a variety of public genetic repositories. Here I illustrate this challenge by analyzing the gene for human insulin-like growth factor 2 (IGF2) through the lens of several databases. IGF2, a 67-amino acid secreted peptide, is essential for normal prenatal growth, and is involved in other physiological and pathophysiological processes in humans...
February 2, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29413895/epigenetic-alterations-contribute-to-promoter-activity-of-imprinting-gene-igf2
#16
Qi-Fan Zheng, Bin Xu, Hui-Min Wang, Li-Hong Ding, Jin-Yang Liu, Ling-Yu Zhu, Huan Qiu, Li Zhang, Guang-Yi Ni, Jing Ye, Shu-Bin Gao, Guang-Hui Jin
The expression of insulin-like growth factor 2 (IGF2), a classical imprinting gene, didn't completely correlate with its imprinting profiles in hepatocellular carcinoma (HCC). The mechanistic importance of promoter activity in regulation of IGF2 has not been fully clarified. Here we show that histone 3 lysine 4 trimethylation (H3K4me3) modified by menin-MLL complex of IGF2 promoter contributes to promoter activity of IGF2. The strong binding of menin and abundant H3K4me3 at the DNA demethylated P3/4 promoters were observed in Hep3B cells with the robust expression of IGF2...
January 31, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29412735/analysis-of-h19-igf2-methylation-status-in-the-sperm-of-cloned-goats-and-their-offspring
#17
Yongjie Wan, Mingtian Deng, Guomin Zhang, Caifang Ren, Zifei Liu, Feng Wang
The H19/insulin-like growth factor 2 (Igf2) gene plays vital roles during development, but their expression and methylation status have not been elucidated in male cloned animals. In this study, we investigated the methylation status of imprinting control region (ICR) and differentially methylated region (DMR) of the H19/Igf2 locus in the sperm and testes of naturally reproduced Saanen dairy goats (NG, control), transgenic cloned dairy goats (CG), and the first generation of mating the CGs with the female NGs (FG)...
February 2018: Cellular Reprogramming
https://www.readbyqxmd.com/read/29401624/insulin-and-igf1-receptors-are-essential-for-the-development-and-steroidogenic-function-of-adult-leydig-cells
#18
Yasmine Neirijnck, Pierre Calvel, Karen R Kilcoyne, Françoise Kühne, Isabelle Stévant, Richard J Griffeth, Jean-Luc Pitetti, Silvana A Andric, Meng-Chun Hu, François Pralong, Lee B Smith, Serge Nef
The insulin family of growth factors (insulin, IGF1, and IGF2) are critical in sex determination, adrenal differentiation, and testicular function. Notably, the IGF system has been reported to mediate the proliferation of steroidogenic cells. However, the precise role and contribution of the membrane receptors mediating those effects, namely, insulin receptor (INSR) and type-I insulin-like growth factor receptor (IGF1R), have not, to our knowledge, been investigated. We show here that specific deletion of both Insr and Igf1r in steroidogenic cells in mice leads to severe alterations of adrenocortical and testicular development...
January 24, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29376352/mitotically-stable-modification-of-dna-methylation-in-igf2-h19-imprinting-control-region-is-associated-with-activated-hepatic-igf2-expression-in-offspring-rats-from-betaine-supplemented-dams
#19
Shu Yang, Nannan Zhao, Yang Yang, Yun Hu, Haibo Dong, Ruqian Zhao
The growth-promoting action of betaine involves activation of GH/IGF-1 signaling, yet it remains unclear whether insulin-like growth factor 2 (IGF2), an imprinting gene, is affected by maternal dietary betaine supplementation. In this study, F1 offspring rats derived from dams fed basal or betaine-supplemented diet were examined at D21 and D63. Maternal betaine significantly upregulated the hepatic expression of IGF2 mRNA and protein in offspring rats at both D21 and D63, which was accompanied by enhanced hepatic IGF2 immunoreactivity and elevated serum IGF-2 level...
January 28, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29348399/insulin-like-growth-factor-2-is-a-key-mitogen-driving-liver-repopulation-in-mice
#20
Min-Jun Wang, Fei Chen, Qing-Gui Liu, Chang-Cheng Liu, Hao Yao, Bing Yu, Hai-Bin Zhang, He-Xin Yan, Yibiao Ye, Tao Chen, Kirk J Wangensteen, Xin Wang, Yi-Ping Hu, Zhi-Ying He
Hepatocyte transplantation holds great promise as an alternative to orthotopic organ transplantation in the treatment of liver diseases. However, obtaining clinically meaningful levels of liver repopulation has not been achieved because the mechanisms regulating hepatocyte proliferation in recipient livers have not yet been well characterized. In the mouse model of Hereditary Tyrosinemia Type I, the fumarylacetoacetate hydrolase-deficient (Fah-/- ) mouse, we found gradually increasing expression level of insulin-like growth factor 2 (IGF2) in the hepatocytes of host livers...
January 18, 2018: Cell Death & Disease
keyword
keyword
91406
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"