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Glutamine AND IGF2

Takatsune Shimizu, Eiji Sugihara, Sayaka Yamaguchi-Iwai, Sakura Tamaki, Yuko Koyama, Walied Kamel, Arisa Ueki, Tomoki Ishikawa, Tatsuyuki Chiyoda, Satoru Osuka, Nobuyuki Onishi, Hiroko Ikeda, Junzo Kamei, Koichi Matsuo, Yumi Fukuchi, Toshihiro Nagai, Junya Toguchida, Yoshiaki Toyama, Akihiro Muto, Hideyuki Saya
Osteosarcoma is a malignant bone tumor in children and adolescents characterized by intrinsic therapeutic resistance. The IGF2 is expressed at elevated levels in osteosarcoma after treatment with chemotherapy, prompting an examination of its functional contributions to resistance. We found that continuous exposure to IGF2 or insulin in the absence of serum created a dormant growth state in osteosarcoma cells that conferred resistance to various chemotherapeutic drugs in vitro. Mechanistic investigations revealed that this dormant state correlated with downregulation of downstream signaling by the IGF1 receptor, heightened cell survival, enhanced autophagy, and the presence of extracellular glutamine...
November 15, 2014: Cancer Research
Honey Modi, Marion Cornu, Bernard Thorens
IGF2 is an autocrine ligand for the beta cell IGF1R receptor and GLP-1 increases the activity of this autocrine loop by enhancing IGF1R expression, a mechanism that mediates the trophic effects of GLP-1 on beta cell mass and function. Here, we investigated the regulation of IGF2 biosynthesis and secretion. We showed that glutamine rapidly and strongly induced IGF2 mRNA translation using reporter constructs transduced in MIN6 cells and primary islet cells. This was followed by rapid secretion of IGF2 via the regulated pathway, as revealed by the presence of mature IGF2 in insulin granule fractions and by inhibition of secretion by nimodipine and diazoxide...
November 14, 2014: Journal of Biological Chemistry
Chi-Hun Park, Young-Hee Jeong, Yeun-Ik Jeong, Jeong-Woo Kwon, Taeyoung Shin, Sang-Hwan Hyun, Eui-Bae Jeung, Nam-Hyung Kim, Sang-Kyo Seo, Chang-Kyu Lee, Woo-Suk Hwang
To determine whether exogenous amino acids affect gene transcription patterns in parthenogenetic porcine embryos, we investigated the effects of amino acid mixtures in culture medium. Parthenogenetic embryos were cultured in PZM3 medium under four experimental conditions: 1) control (no amino acids except L-glutamine and taurine); 2) nonessential amino acids (NEAA); 3) essential amino acids (EAA); and 4) NEAA and EAA. The rate of development of embryos to the four-cell stage was not affected by treatment. However, fewer (P<0...
2014: PloS One
Haitham G Abo-Al-Ela, Mohammed Abu El-Magd, Abeer F El-Nahas, Ali A Mansour
Insulin-like growth factor 2 (IGF2) plays an important role in muscle growth and it might be used as a marker for the growth traits selection strategies in farm animals. The objectives of this study were to detect polymorphisms in exon 10 of IGF2 and to determine associations between these polymorphisms and growth traits in Egyptian water buffalo. PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing methods were used to detect any prospective polymorphism. A novel single nucleotide polymorphism (SNP), C287A, was detected...
August 2014: Tropical Animal Health and Production
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