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Felix Lange, Niels Pfennigwerth, Rainer Hartl, Heidrun Kerschner, Dagmar Achleitner, Sören G Gatermann, Martin Kaase
Objectives: To identify and characterize a novel MBL gene conferring carbapenem resistance to an isolate of Enterobacter cloacae from Austria. Methods: The novel MBL gene was heterologously expressed in Escherichia coli TOP10 to conduct comparative MIC studies and biochemical assays. Furthermore, WGS was performed using Illumina MiSeq and Oxford Nanopore MinION instruments to analyse the genetic environment of the novel MBL gene. Results: The novel MBL showed highest sequence homology to a predicted MBL precursor from the marine bacterium Rheinheimera pacifica and hence belongs to Ambler subgroup B3...
June 11, 2018: Journal of Antimicrobial Chemotherapy
Sofía Barrera, Paul Cardenas, Jay P Graham, Gabriel Trueba
Intestinal bacteria carry antimicrobial resistance (AMR) genes in mobile genetic elements which have the potential to spread to bacteria in other animal hosts including humans. In fecal matter, Escherichia coli can continue to multiply for 48 hr after being excreted, and in certain environments, E. coli survive long periods of time. It is unclear the extent to which AMR in E. coli changes in the environment outside of its host. In this study, we analyzed changes in the population structure, plasmid content, and AMR patterns of 30 E...
June 12, 2018: MicrobiologyOpen
Angela Gomez-Simmonds, Stephania Stump, Marla J Giddins, Medini K Annavajhala, Anne-Catrin Uhlemann
Treatment options for carbapenem-resistant Enterobacteriaceae (CRE) are limited. While Klebsiella pneumoniae harboring bla KPC account for most CRE, recent evidence points to increasing diversification of CRE. We determined whether CRE species and antibiotic resistance genotype influence response to relebactam (REL), a novel beta-lactamase inhibitor with class A/C activity, combined with imipenem(IMI)/cilastatin. We carried out broth microdilution testing to IMI alone or in the presence of 4 μg/mL REL in 154 clinical isolates collected at a New York City hospital with high prevalence of bla KPC including Enterobacter spp...
June 11, 2018: Antimicrobial Agents and Chemotherapy
Tellez German Alberto, Zapata Jesica Alejandra, Toro Lily Johanna, Henao Diana Carolina, Bedoya Juan Pablo, Rivera Juan David, Trujillo Juan Valentin, Rivas Bruno, Richard Onalbi Hoyos Lopez, Castano Jhon Carlos
Maggots from the Lucilia sp. genus are used for debridement of infected and necrotic wounds. Broad-spectrum antimicrobial activity has been described in the excretion/secretions (ES1 ) of these larvae. This study identifies the genetic sequence of a cecropin-like antimicrobial peptide from Lucilia eximia. Total RNA was extracted and used for PCR-RACE amplification of a cecropin, the native peptide was immunolocalized in the tissues and secretions of the larvae, and a synthetic analog was used to explore its antimicrobial, cytotoxic, LPS neutralizing and wound-healing activities in vitro...
June 8, 2018: Acta Tropica
Christopher M Crittenden, Carmen M Herrera, Peggy E Williams, Dante P Ricci, Lee R Swem, M Stephen Trent, Jennifer S Brodbelt
Structural characterization of lipid A from Gram-negative bacteria remains a significant challenge, especially with respect to localizing modifications of the phosphate groups typically found on the reducing and non-reducing ends of the β-1',6-linked glucosamine disaccharide backbone of lipid A. As reported here, combining traditional collisional activated dissociation (CAD) and ultraviolet photodissociation (UVPD) in a hybrid MS3 approach facilitates identification and localization of substituents of the phosphate groups...
June 8, 2018: Analyst
Marta Dec, Anna Nowaczek, Renata Urban-Chmiel, Dagmara Stępień-Pyśniak, Andrzej Wernicki
Campylobacteriosis is currently the most frequent zoonosis in humans and the main source of infection is contaminated poultry meat. As chickens are a natural host for Campylobacter species, one strategy to prevent infection in humans is to eliminate these bacteria on poultry farms. A study was conducted to evaluate the probiotic potential of 46 Lactobacillus isolates from chickens faeces or cloacae. All lactobacilli were able to produce active compounds on solid media with antagonistic properties against C...
June 6, 2018: Journal of Veterinary Medical Science
Sally Tohamy Tohamy, Khaled Mohamed Aboshanab, Hadir Ahmed El-Mahallawy, Mona R El-Ansary, Salwa Selim Afifi
Introduction: Bloodstream infections with multidrug-resistant (MDR) Gram-negative bacteria (GNB) are among the most frequent complications in immunocompromised cancer patients because of their considerable morbidity and mortality. Several guidelines on antimicrobial therapy have addressed empirical treatment for such serious infections; however, the emergence of microbial resistance has become a significant problem worldwide. Materials and methods: In this study, starting from November 2015 to October 2016, a total of 529 blood specimens were collected from febrile neutropenic cancer patients at a tertiary care cancer hospital in Egypt...
2018: Infection and Drug Resistance
Maximiliano Figueroa, Valentina Fernandez, Mauricio Arenas-Salinas, Diego Ahumada, Claudia Muñoz-Villagrán, Fabián Cornejo, Esteban Vargas, Mauricio Latorre, Eduardo Morales, Claudio Vásquez, Felipe Arenas
Microbes are suitable candidates to recover and decontaminate different environments from soluble metal ions, either via reduction or precipitation to generate insoluble, non-toxic derivatives. In general, microorganisms reduce toxic metal ions generating nanostructures (NS), which display great applicability in biotechnological processes. Since the molecular bases of bacterial reduction are still unknown, the search for new -environmentally safe and less expensive- methods to synthesize NS have made biological systems attractive candidates...
2018: Frontiers in Microbiology
Ram Chandra, Pooja Sharma, Sangeeta Yadav, Sonam Tripathi
Effluent discharged from the pulp and paper industry contains various refractory and androgenic compounds, even after secondary treatment by activated processes. Detailed knowledge is not yet available regarding the properties of organic pollutants and methods for their bioremediation. This study focused on detecting residual organic pollutants of pulp and paper mill effluent after biological treatment and assessing their degradability by biostimulation. The major compounds identified in the effluent were 2,3,6-trimethylphenol, 2-methoxyphenol (guaiacol), 2,6-dimethoxyphenol (syringol), methoxycinnamic acid, pentadecane, octadecanoic acid, trimethylsilyl ester, cyclotetracosane, 5,8-dimethoxy-6-methyl-2,4-bis(phenylmethyl)napthalen-1-ol, and 1,2-benzendicarboxylic acid diisononyl ester...
2018: Frontiers in Microbiology
Mohamed Rhouma, Salma Bessalah, Imed Salhi, William Thériault, John Morris Fairbrother, Philippe Fravalo
Camels (Camelus dromedarius) are known to harbor multidrug resistant Gram-negative bacteria and to be involved in the transmission of various microorganisms to humans. Data on the occurrence of colistin resistant Escherichia coli as well as mobilized colistin resistance (mcr) genes in camels are lacking. We investigated the presence of colistin resistance and mcr (1-2) genes in E. coli from the feces of camels in Tunisia. Presumptive E. coli isolates from camel-calves in southern Tunisia were qualitatively screened for growth on Mueller-Hinton agar supplemented with 2 mg/L of colistin...
June 5, 2018: Acta Veterinaria Scandinavica
Angela Dickson, Preeti Kondal, Lou Hilken, Michael Helgesen, William Sjolin, Dennis Jensen
During an investigation of positive environmental cultures with Enterobacter cloacae from an endobronchial ultrasound scope, possible pseudotransmission was discovered between 2 patients. All reprocessing steps were adhered to and the original equipment manufacture's quality control assessment of the scope could not determine the root cause. Our findings appear to be the first documented case of pseudotransmission from an endobronchial ultrasound scope and suggest bacterial transmission may exist in endoscopes without an elevator channel...
May 30, 2018: American Journal of Infection Control
Yoon-Kyoung Hong, Ji-Young Lee, Kwan Soo Ko
We investigated the colistin resistance rate among 356 Enterobacter spp. clinical isolates from eight hospitals in Korea. Antibiotic susceptibility testing was performed by broth microdilution. While 51 of 213 (23.9%) Enterobacter cloacae isolates were colistin-resistant, only six of 143 (4.2%) E. aerogenes isolates showed resistance. We also identified the skip well phenotype in eight E. cloacae and three E. aerogenes isolates. Multilocus sequence typing for E. cloacae and randomly amplified polymorphic DNA analysis and enterobacterial repetitive intergenic consensus PCR for E...
June 2018: Journal of Microbiology / the Microbiological Society of Korea
Paola Aparecida Alves Azevedo, João Pedro Rueda Furlan, Mariana Oliveira-Silva, Rafael Nakamura-Silva, Carolina Nogueira Gomes, Karen Regina Carim Costa, Eliana Guedes Stehling, André Pitondo-Silva
Enterobacter cloacae and E. aerogenes have been increasingly reported as important opportunistic pathogens. In this study, a high prevalence of multi-drug resistant isolates from Brazil, harboring several β-lactamase encoding genes was found. Several virulence genes were observed in E. aerogenes, contrasting with the E. cloacae isolates which presented none.
May 21, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
Anniina Keskitalo, Eveliina Munukka, Raine Toivonen, Maija Hollmén, Heikki Kainulainen, Pentti Huovinen, Sirpa Jalkanen, Satu Pekkala
Accumulating evidence indicates that gut microbiota plays a significant role in obesity, insulin resistance and associated liver disorders. Family Enterobacteriaceae and especially Enterobacter cloacae strain B29 have been previously linked to obesity and hepatic damage. The underlying mechanisms, however, remain unclear. Therefore, we comprehensively examined the effects of E. cloacae subsp. cloacae (ATCC® 13047™) administration on host metabolism of mice fed with high-fat diet (HFD). C57BL/6N mice were randomly divided into HFD control, chow control, and E...
2018: PloS One
Y N Zhao, H F Wang, J X Liu
Jatropha curcas seed cake is a by-product generated after oil extraction from J. curcas seeds. Although the protein content is high, the cake contains phorbol esters and anti-nutritional factors such as phytates, trypsin inhibitors, lectins, and tannins. Therefore, it cannot be directly used in food or feed. In this study, the toxic compounds and anti-nutrients present in J. curcas seed cake were detoxified by fermentation with Enterobacter Z11, a soil-borne isolate. Solid-state fermentation was undertaken under optimized conditions: deoiled cake, 5...
May 30, 2018: Letters in Applied Microbiology
Angela Gomez-Simmonds, Medini K Annavajhala, Zheng Wang, Nenad Macesic, Yue Hu, Marla J Giddins, Aidan O'Malley, Nora C Toussaint, Susan Whittier, Victor J Torres, Anne-Catrin Uhlemann
Recent reports have established the escalating threat of carbapenem-resistant Enterobacter cloacae complex (CREC). Here, we demonstrate that CREC has evolved as a highly antibiotic-resistant rather than highly virulent nosocomial pathogen. Applying genomics and Bayesian phylogenetic analyses to a 7-year collection of CREC isolates from a northern Manhattan hospital system and to a large set of publicly available, geographically diverse genomes, we demonstrate clonal spread of a single clone, ST171. We estimate that two major clades of epidemic ST171 diverged prior to 1962, subsequently spreading in parallel from the Northeastern to the Mid-Atlantic and Midwestern United States and demonstrating links to international sites...
May 29, 2018: MBio
Mengmeng Ke, Bandikari Ramesh, Yian Hang, Ziduo Liu
Esterases are one of the most important industrial enzymes. Here, a novel estA was cloned from Enterobacter sp. and characterized. The sequence alignment results showed that it was a novel esterase. The purified EstA had a molecular weight of 26 KDa with an optimum temperature and pH of 40 °C and 9.0. EstA retained >70% activity between 0 °C and 20 °C, indicating it was a low temperature active enzyme. EstA exhibited low activity after incubation at 45 °C for 120 min or 50 °C for 30 min...
May 26, 2018: International Journal of Biological Macromolecules
Guillaume Miltgen, Rémy A Bonnin, Catherine Avril, Thierry Benoit-Cattin, Daniel Martak, Alexandre Leclaire, Nicolas Traversier, Bénédicte Roquebert, Marie-Christine Jaffar-Bandjee, Nathalie Lugagne, Laurent Filleul, Marion Subiros, Anne-Marie de Montera, Pascal Cholley, Michelle Thouverez, Laurent Dortet, Xavier Bertrand, Thierry Naas, Didier Hocquet, Olivier Belmonte
The spread of carbapenemase-producing Enterobacteriaceae (CPE) in the Southwest Indian Ocean islands is poorly known. We describe here an outbreak of colistin-resistant Enterobacter cloacae harboring blaIMI-1 in the French overseas department of Mayotte. Between October 2015 and January 2017, all isolates of imipenem-non-susceptible E. cloacae at the Mayotte Medical Center and University Hospital of Reunion Island were screened for carbapenemase production. Positive isolates were typed by pulsed-field gel electrophoresis and WGS-based MLST and all β-lactamase genes identified by PCR and sequencing...
May 25, 2018: International Journal of Antimicrobial Agents
Simon Garinet, Vincent Fihman, Hervé Jacquier, Stéphane Corvec, Le Monnier Alban, Thomas Guillard, Vincent Cattoir, Jean-Ralph Zahar, Paul-Louis Woerther, Etienne Carbonnelle, Alain Wargnier, Solen Kernéis, Philippe C Morand
OBJECTIVES: The Enterobacter cloacae complex (Ecc), routinely referred to as "E. cloacae" in clinical microbiology, encompasses several species with 12 genetic clusters and one sequence crowd that can be identified based on hsp60 sequencing. Little is known about the pathogenicity and distribution of resistance to antibiotics among the Ecc. METHODS AND RESULTS: In this prospective multicentre study, a total of 193 Ecc clinical isolates were collected from 10 academic hospitals distributed nationally across France and identified at the genetic cluster level on the basis of hsp60 sequencing...
May 26, 2018: Journal of Infection
Qada Benameur, Hassiba Tali-Maamar, Farida Assaous, Badia Guettou, Meki Boutaiba Benklaouz, Kheira Rahal, Meriem-Hind Ben-Mahdi
Aim: Multidrug-resistant (MDR) Enterobacteriaceae have frequently been reported, in both human and veterinary medicine, from different parts of the world as a consequence of antibiotic usage. However, there is a lack of published data regarding antimicrobial resistance in non- Escherichia coli ( E. coli ) Enterobacteriaceae from animals in Algeria. This study aimed to evaluate the frequency of resistance to antibiotics with a focus on quinolones and to investigate the presence of qnr genes inEnterobacteriaceaeof poultry origin...
April 2018: Veterinary World
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