keyword
MENU ▼
Read by QxMD icon Read
search

Cloacae

keyword
https://www.readbyqxmd.com/read/29214986/antibiotic-resistance-in-enterobacter-cloacae-strains-with-derepressed-partly-derepressed-inducible-ampc-and-extendedspectrum-beta-lactamases-in-zenica-doboj-canton-bosnia-and-herzegovina
#1
Selma Uzunović, Amir Ibrahimagić, Branka Bedenić
Aim To investigate the prevalence of derepressed/partly derepressed/inducible and ESBL/AmpC-producing Enterobacter cloacae isolates and treatment options for infections associated with those isolates. Methods Antibiotic susceptibility was determined by disc diffusion and broth microdilution according to CLSI guidelines. Doubledisk synergy test (DDST) was performed in order to screen for ESBLs and combined disk test with phenylboronic acid to detect AmpC β -lactamases. PCR was used to detect blaESBL/blacarb genes...
February 1, 2018: Medicinski Glasnik
https://www.readbyqxmd.com/read/29211819/genomic-investigation-of-a-putative-endoscope-associated-carbapenem-resistant-enterobacter-cloacae-outbreak-reveals-a-wide-diversity-of-circulating-strains-and-resistance-mutations
#2
Shawn E Hawken, Laraine L Washer, Christopher L Williams, Duane W Newton, Evan S Snitkin
A genomic epidemiologic investigation of a putative carbapenem-resistant Enterobacter cloacae outbreak revealed few plausible instances of nosocomial transmission, highlighting instead the frequent importation of E. cloacae into our hospital. Searching for genetic determinants of carbapenem resistance demonstrated that most resistance is due to convergent mutations in phylogenetically diverse E. cloacae.
October 26, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29210028/antimicrobial-peptides-sourced-from-post-butter-processing-waste-yak-milk-protein-hydrolysates
#3
Jinjin Pei, Hai Jiang, Xinsheng Li, Wengang Jin, Yanduo Tao
Yak butter is one of the most important foods for the Tibetan people. Of note, its production yields waste yak milk as a by-product. In this work, waste yak milk protein hydrolysates made via Pepsin hydrolysis were shown to have antimicrobial activity. Furthermore, an innovative method of magnetic liposome adsorption combined with reversed-phase high performance liquid chromatography (RP-HPLC) was developed to screen for and purify the antimicrobial peptides. Two antimicrobial peptides were obtained and their amino acid sequences were determined by N-sequencing, namely Arg-Val-Met-Phe-Lys-Trp-Ala and Lys-Val-Ile-Ser-Met-Ile...
December 6, 2017: AMB Express
https://www.readbyqxmd.com/read/29205893/measuring-the-gut-microbiome-in-birds-comparison-of-faecal-and-cloacal-sampling
#4
Elin Videvall, Maria Strandh, Anel Engelbrecht, Schalk Cloete, Charlie K Cornwallis
The gut microbiomes of birds and other animals are increasingly being studied in ecological and evolutionary contexts. Numerous studies on birds and reptiles have made inferences about gut microbiota using cloacal sampling, however, it is not known whether the bacterial community of the cloaca provides an accurate representation of the avian gut microbiome. We examined the accuracy with which cloacal swabs and faecal samples measure the microbiota in three different parts of the gastrointestinal tract (ileum, caecum, and colon) using a case study on juvenile ostriches, Struthio camelus, and high-throughput 16S rRNA sequencing...
December 4, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29205135/a-hemin-auxotrophic-enterobacter-cloacae-clinical-isolate-with-increased-resistance-to-carbapenems-and-aminoglycosides
#5
Tatsuya Tada, Kohei Uechi, Isamu Nakasone, Zenji Miyazato, Takashi Shinzato, Kayo Shimada, Mitsuhiro Tsuchiya, Teruo Kirikae, Jiro Fujita
Small-colony variants (SCVs) were obtained from an Enterobacter cloacae clinical isolate in Okinawa, Japan. One variant showed auxotrophy for hemin with a deletion of 20 365 nucleotides, dosC-ydiK-mmuP-mmuM-tauA-tauB-tauC-tauD-hemB-yaiT-yaiV-ampH-yddQ-sbmA-yaiW-yaiY-yaiZ, including hemB, and was more resistant to aminoglycosides and carbapenems, but more susceptible to aztreonam, than the parent strain.
December 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29203488/epidemiology-of-carbapenem-resistant-enterobacteriaceae-infections-report-from-china-cre-network
#6
Yawei Zhang, Qi Wang, Yuyao Yin, Hongbin Chen, Longyang Jin, Bing Gu, Liangyi Xie, Chunxia Yang, Xiaobo Ma, Huayin Li, Wei Li, Xiaoqian Zhang, Kang Liao, Sijin Man, Shifu Wang, Hainan Wen, Binbin Li, Zhusheng Guo, Jinjing Tian, Fengyan Pei, Li Liu, Lijun Zhang, Cuimei Zou, Tongping Hu, Jun Cai, Hong Yang, Jiaming Huang, Xianghong Jia, Wenhui Huang, Bin Cao, Hui Wang
Carbapenem-resistant Enterobacteriaceae (CRE) infection is highly endemic in China, but estimates of infection burden are lacking. We established incidence of CRE infection from a multicenter study that covered 25 tertiary hospitals in 14 provinces. CRE cases defined as carbapenem non-susceptible C. freundii, E. coli, E. cloacae, or K. pneumoniae infections during January to December 2015 were collected and reviewed from medical records. Antimicrobial susceptibility testing and carbapenemase genes identification were performed...
December 4, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29202138/effects-and-time-kill-assessment-of-amoxicillin-used-in-combination-with-chloramphenicol-against-bacteria-of-clinical-importance
#7
Olufunmiso O Olajuyigbe, Roger M Coopoosamy, Anthony J Afolayan
With the emergence of multidrug-resistant organisms in an era when drug development faces challenges causing pharmaceutical companies to curtail or abandon research on anti-infective agents, the use of combined existing antimicrobial agents may be an alternative. This study evaluated the effects of combining amoxicillin and chloramphenicol, to which many bacteria have become resistant, in vitro against Gram positive and Gram negative bacteria by agar diffusion, checkerboard and time-kill assays. The test isolates were susceptible to amoxicillin with minimum inhibitory concentrations (MICs) ranging between 0...
November 30, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29201017/characterization-of-metagenomes-in-urban-aquatic-compartments-reveals-high-prevalence-of-clinically-relevant-antibiotic-resistance-genes-in-wastewaters
#8
Charmaine Ng, Martin Tay, Boonfei Tan, Thai-Hoang Le, Laurence Haller, Hongjie Chen, Tse H Koh, Timothy M S Barkham, Karina Y-H Gin
The dissemination of antimicrobial resistance (AMR) is an escalating problem and a threat to public health. Comparative metagenomics was used to investigate the occurrence of antibiotic resistant genes (ARGs) in wastewater and urban surface water environments in Singapore. Hospital and municipal wastewater (n = 6) were found to have higher diversity and average abundance of ARGs (303 ARG subtypes, 197,816 x/Gb) compared to treated wastewater effluent (n = 2, 58 ARG subtypes, 2,692 x/Gb) and surface water (n = 5, 35 subtypes, 7,985 x/Gb)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29195767/ndm-1-encoded-by-a-pndm-hn380-like-plasmid-pndm-bj03-in-clinical-enterobacter-cloacae
#9
Yang Lü, Wei Liu, Hui Liang, Shulong Zhao, Wei Zhang, Jia Liu, Cheng Jin, Hongyan Hu
A carbapenemase-producing Enterobacter cloacae hhy03 with a blaNDM-1 and blaSHV-12-coharboring plasmid was isolated from a sputum specimen of a patient. This is the third nucleotide sequence report of blaNDM-1-harboring plasmid from Enterobacter cloacae that have caused lethal infections in China, indicating the spread of NDM-1 by IncX3 plasmid between Enterobacteriaceae.
November 7, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29191217/multi-level-comparisons-of-cloacal-skin-feather-and-nest-associated-microbiota-suggest-considerable-influence-of-horizontal-acquisition-on-the-microbiota-assembly-of-sympatric-woodlarks-and-skylarks
#10
H Pieter J van Veelen, Joana Falcao Salles, B Irene Tieleman
BACKGROUND: Working toward a general framework to understand the role of microbiota in animal biology requires the characterisation of animal-associated microbial communities and identification of the evolutionary and ecological factors shaping their variation. In this study, we described the microbiota in the cloaca, brood patch skin and feathers of two species of birds and the microbial communities in their nest environment. We compared patterns of resemblance between these microbial communities at different levels of biological organisation (species, individual, body part) and investigated the phylogenetic structure to deduce potential microbial community assembly processes...
December 1, 2017: Microbiome
https://www.readbyqxmd.com/read/29185939/flavobacterium-naphthae-sp-nov-isolated-from-oil-contaminated-soil
#11
Dhiraj Kumar Chaudhary, Jaisoo Kim
During a study of petroleum hydrocarbon-degrading bacteria, a yellow-coloured, Gram-stain-negative, non-motile and rod-shaped bacterium, designated strain Brt-MT, was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal. Strain Brt-MT was able to grow at 15-45 °C, pH 5.0-9.0 and 0-1 % (w/v) NaCl concentration. The strain was characterized by multiple taxonomic approaches. Based on 16S rRNA gene sequence analysis, strain Brt-MT belonged to the genus Flavobacterium and shared highest sequence similarity with Flavobacterium cloacae wh15T (95...
November 29, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29181448/direct-pcr-offers-a-fast-and-reliable-alternative-to-conventional-dna-isolation-methods-for-gut-microbiomes
#12
Elin Videvall, Maria Strandh, Anel Engelbrecht, Schalk Cloete, Charlie K Cornwallis
The gut microbiome of animals is emerging as an important factor influencing ecological and evolutionary processes. A major bottleneck in obtaining microbiome data from large numbers of samples is the time-consuming laboratory procedures required, specifically the isolation of DNA and generation of amplicon libraries. Recently, direct PCR kits have been developed that circumvent conventional DNA extraction steps, thereby streamlining the laboratory process by reducing preparation time and costs. However, the reliability and efficacy of direct PCR for measuring host microbiomes have not yet been investigated other than in humans with 454 sequencing...
November 2017: MSystems
https://www.readbyqxmd.com/read/29180323/-pathogen-analysis-in-patients-with-diabetic-foot-osteomyelitis-using-16s-rrna-high-throughput-sequencing
#13
Ping Hu, Meng-Chen Zou, Ying Cao, Yan-Ling Pan, Xiang-Rong Luo, Ya Jiang, Yao-Ming Xue, Fang Gao
OBJECTIVE: To analyze the characteristics of pathogenic microorganisms in the infected bone tissues in patients with diabetic foot osteomyelitis (DFO) using 16S rRNA high-throughput sequencing to facilitate rapid and accurate detection of pathogens and effective infection control. METHODS: Between September, 2016 and April, 2017, 16 patients with DFO were admitted in our department and infected bone specimens were obtained during debridement. The pathogenic microorganisms in the specimens were identified using both 16S rRNA high-throughput sequencing and automatic blood culture analyzer, and the characteristics of the microflora were analyzed based on 16S rRNA sequencing data in comparison with the results of blood culture...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29177441/de-novo-mutations-in-caudal-type-homeo-box-transcription-factor-2-cdx2-in-patients-with-persistent-cloaca
#14
Jacob S J Hsu, Manting So, Clara S M Tang, Karim Anwarul, Robert M Porsch, Carol Wong, Michelle Yu, Fanny Yeung, Huimin Xia, Ruizhong Zhang, Stacey S Cherny, Patrick H Y Chung, K Y Wong Kenneth, Pak C Sham, Ngoc Diem Ngo, Miaoxin Li, Paul K H Tam, Vincent C H Lui, Maria-Mercè Garcia-Barcelo
The cloaca is an embryonic cavity that is divided into the urogenital sinus and rectum upon differentiation of the cloacal epithelium triggered by tissue-specific transcription factors including CDX2. Defective differentiation leads to persistent cloaca in humans (PC), a phenotype recapitulated in Cdx2 mutant mice. PC is linked to hypo/hyper-vitaminosis A. Although no gene has ever been identified, there is a strong evidence for a genetic contribution to PC. We applied whole-exome sequencing and copy-number-variants analyses to 21 PC patients and their unaffected parents...
November 21, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29175778/characterization-of-pathogens-involved-in-ventilator-associated-pneumonia-in-surgical-and-medical-intensive-care-units-a-single-center-experience
#15
Saghir Ahmad, Nafees Bacha, Jehan Bakht, Jawad Ahmed
In the present study 60 samples were collected from lower respiratory tract of patients suffering from Ventilator Associated Pneumonia (VAP) admitted in surgical and medical Intensive Care Units (ICUs) of Khyber Teaching Hospital, Peshawar, Pakistan. Recovered pathogens were characterized and their susceptibility pattern against commonly used antibacterial agents investigated. Most frequent bacterial pathogen found was methicillin- resistant Staphylococcus aureus (MRSA) (40%) followed by members of Enterobacteriaceae (22%; of which Escherichia coli (50%), Klebsiella pneumonia (30%), Enterobacter cloacae (10%) and Citrobacter freundii (10%), Pseudomonas aeruginosa 20% and Acinetobacter baumannii 18%...
November 2017: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29173582/staphylococcus-agnetis-a-potential-pathogen-in-broiler-breeders
#16
Louise Ladefoged Poulsen, Ida Thøfner, Magne Bisgaard, Rikke Heidemann Olsen, Jens Peter Christensen, Henrik Christensen
In this study, four broiler parent flocks have been followed from the onset of the production period (week 20) until slaughter (week 60). Every week, approximately ten dead broiler breeders, randomly selected among birds dead on their own, were collected and subjected to a full post mortem analysis including bacteriological examination. In total 997 breeders were investigated and for the first time Staphylococcus agnetis was isolated in pure culture from cases of endocarditis and septicemia from 16 broiler breeders...
December 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29168057/-1-h-15-n-and-13-c-backbone-resonance-assignments-of-pentaerythritol-tetranitrate-reductase-from-enterobacter-cloacae-pb2
#17
Andreea I Iorgu, Nicola J Baxter, Matthew J Cliff, Jonathan P Waltho, Sam Hay, Nigel S Scrutton
Pentaerythritol tetranitrate reductase (PETNR) is a flavoenzyme possessing a broad substrate specificity and is a member of the Old Yellow Enzyme family of oxidoreductases. As well as having high potential as an industrial biocatalyst, PETNR is an excellent model system for studying hydrogen transfer reactions. Mechanistic studies performed with PETNR using stopped-flow methods have shown that tunneling contributes towards hydride transfer from the NAD(P)H coenzyme to the flavin mononucleotide (FMN) cofactor and fast protein dynamics have been inferred to facilitate this catalytic step...
November 22, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/29156244/isolation-and-characterization-of-polyvalent-bacteriophages-infecting-multi-drug-resistant-salmonella-serovars-isolated-from-broilers-in-egypt
#18
Mayada Mahmoud, Ahmed Askora, Ahmed Barakat Barakat, Omar El-Farouk Rabie, Sayed Emam Hassan
In this study, we isolated and characterized three phages named as Salmacey1, Salmacey2 and Salmacey3, infecting multi drug resistant Salmonella serovars isolated from broilers in Egypt. The most prevalent Salmonella serovars were S. typhimurium, S. enteritidis, and S. kentucky. All these Salmonella serovars were found to be resistant to more than two of the ten antimicrobial agents tested. Only S. kentucky was found to be resistant to seven antimicrobial agents. Examination of these phage particles by transmission electron microscopy (TEM), demonstrated that two phages (Salmacey1, Salmacey2) were found to belong to family Siphoviridae, and Salmacey3 was assigned to the family Myoviridae...
November 14, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29155392/green-synthesised-zinc-oxide-nanostructures-through-periploca-aphylla-extract-shows-tremendous-antibacterial-potential-against-multidrug-resistant-pathogens
#19
Fazal Abbas, Qaisar Maqbool, Mudassar Nazar, Nyla Jabeen, Syed Zaheer Hussain, Sadaf Anwaar, Nasir Mehmood, Muhammad Saleem Sheikh, Talib Hussain, Sidra Iftikhar
To grapple with multidrug resistant bacterial infections, implementations of antibacterial nanomedicines have gained prime attention of the researchers across the globe. Nowadays, zinc oxide (ZnO) at nano-scale has emerged as a promising antibacterial therapeutic agent. Keeping this in view, ZnO nanostructures (ZnO-NS) have been synthesised through reduction by P. aphylla aqueous extract without the utilisation of any acid or base. Structural examinations via scanning electron microscopy (SEM) and X-ray diffraction have revealed pure phase morphology with highly homogenised average particle size of 18 nm...
December 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29150374/occurrence-of-enterobacter-hormaechei-carrying-blandm-1-and-blakpc-2-in-china
#20
Biwei Yang, Yu Feng, Alan McNally, Zhiyong Zong
Three carbapenem-resistant clinical isolates of the Enterobacter cloacae complex (ECC) were recovered from different patients in a hospital. All 3 isolates carried 2 carbapenemase genes blaKPC-2 and blaNDM-1. A study was performed to characterize their relatedness and to investigate possible links among the patients. Whole genome sequencing revealed that the isolates were Enterobacter hormaechei and belonged to ST177 of the ECC. There were 19-142 single nucleotide polymorphisms (SNPs) between the isolates, suggesting that the isolates were likely from a central reservoir, which might have existed for some time...
October 19, 2017: Diagnostic Microbiology and Infectious Disease
keyword
keyword
91375
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"