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https://www.readbyqxmd.com/read/29450810/characterisation-of-a-new-species-of-trichodorus-cobb-1913-triplonchida-trichodoridae-from-iran-based-on-morphological-and-molecular-data
#1
Ramezan Asghari, Ali Eskandari, Zahra Tanha Maafi, Wilfrida Decraemer
Trichodorus zanjanensis n. sp., collected from the rhizosphere of wild almond tree (Amygdalus scoparia Spach) in Zanjan Province, Iran, is described and illustrated based on morphological and molecular data. The new species is characterised by its body length (772-819 μm in males; 704-953 μm in females), and in having medium-sized onchiostyle (60-64 μm in males; 59-65 μm in females) and a short dorsal overlap of the intestine. Males of the new species have two ventromedian cervical papillae posterior to onchiostyle region, ventrally curved spicules, smooth, long and wide manubrium, gradually continuing in a narrower anterior part of the blade provided with bristles and marked from the wider and transversely striated main part of the blade, tapered towards distal end, three ventromedian precloacal supplements, the posterior one (SP1) situated at the level of anterior blade and gubernaculum situated at 24-25 μm from the cloaca...
February 15, 2018: Systematic Parasitology
https://www.readbyqxmd.com/read/29436319/from-dusk-to-dawn-understanding-the-impact-of-ertapenem-resistance-mechanisms-on-the-in-vitro-potency-of-other-drugs-among-enterobacter-cloacae-complex-isolates
#2
Leandro Reus Rodrigues Perez
No abstract text is available yet for this article.
February 13, 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29432912/in-vitro-evaluation-of-new-2-phenoxy-benzo-g-1-2-4-triazolo-1-5-a-quinazoline-derivatives-as-antimicrobial-agents
#3
Hatem A Abuelizz, Rabab A El-Dib, Mohamed Marzouk, Rashad Al-Salahi
Previously, seventeen 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives were prepared and characterized by physicochemical and spectral means. This study was conducted to evaluate their activities in vitro against five Gram-negative and five Gram-positive of clinically pathogenic bacterial strains and ten fungal strains. The antimicrobial activity was assessed, and the minimum inhibitory concentration values of the tested compounds were determined in μg ml-1 , using the diffusion agar technique...
February 9, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29422783/-enterobacter-cloacae-an-emerging-plant-pathogenic-bacterium-affecting-chili-pepper-seedlings
#4
Tanahiri García-González, Hilda Karina Sáenz-Hidalgo, Hilda Victoria Silva-Rojas, Carlos Morales-Nieto, Taca Vancheva, Ralf Koebnik, Graciela Dolores Ávila-Quezada
A previously unreported bacterial disease on chili pepper ( Capsicum annuum L.) seedlings affecting as many as 4% of seedlings was observed in greenhouses in Chihuahua, Mexico (Delicias and Meoqui counties). Initial lesions appeared as irregular small spots on leaves and brown necrosis at margins tips were observed. Later, the spots became necrotic with a chlorotic halo. Advanced disease was associated with defoliation. A Gram negative, rod-shaped bacterium was isolated from diseased chili pepper seedlings...
February 2018: Plant Pathology Journal
https://www.readbyqxmd.com/read/29416290/omphalocele-exstrophy-of-cloaca-imperforate-anus-and-spinal-defect-complex-multiple-major-reconstructive-surgeries-needed
#5
Nada Neel, Mohmoud Salem Tarabay
OEIS complex is a rare combination of serious birth defects including omphalocele, exstrophy of cloaca, imperforate anus, and spinal defects. The aim of managements has shifted from merely providing survival to improve patient outcomes and quality of life with higher level of physical and social independence. Multiple complicated reconstructive surgeries always needed for achieving the goals of treatment. In this case report, we aimed to present our surgical approach for this rare abnormality to achieve functionally and socially acceptable outcome...
January 2018: Urology Annals
https://www.readbyqxmd.com/read/29411729/fecal-carriage-of-extended-spectrum-beta-lactamase-and-ampc-beta-lactamase-producing-enterobacteriaceae-in-a-turkish-community
#6
G Hazirolan, I Mumcuoglu, G Altan, B B Özmen, N Aksu, Z C Karahan
BACKGROUND: Community-acquired infection caused by extended-spectrum beta-lactamase (ESBL)-producing microorganisms has an increasing frequency. AIM: The aim of this study was to determine the fecal carriage of ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in community and to investigate cefotaxime-M (CTX-M) genes among ESBL isolates. MATERIALS AND METHODS: A total of 1402 fecal specimens which were collected from outpatients included in the study...
January 2018: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/29409584/rapid-identification-and-subtyping-of-enterobacter-cloacae-clinical-isolates-using-peptide-mass-fingerprinting
#7
Yi Qian Wang, Di Xiao, Juan Li, Hui Fang Zhang, Bao Qing Fu, Xiao Ling Wang, Xiao Man Ai, Yan Wen Xiong, Jian Zhong Zhang, Chang Yun Ye
OBJECTIVE: To establish a domestic database of Enterobacteria cloacae (E. cloacae), and improve the identification efficiency using peptide mass fingerprinting. METHODS: Peptide mass fingerprinting was used for the identification and subtyping of E. cloacae. Eighty-seven strains, identified based on hsp60 genotyping, were used to construct and evaluate a new reference database. RESULTS: Compared with the original reference database, the identification efficiency and accuracy of the new reference database was greatly improved at the species level...
January 2018: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/29406083/a-targeted-investigation-to-demonstrate-the-freedom-of-west-timor-from-hpai-h5n1
#8
Petrus Malo Bulu, Ian D Robertson, Maria Geong
In early 2004 highly pathogenic avian influenza (HPAI) H5N1 virus caused major outbreaks of disease in poultry in Indonesia. The disease was first reported in West Timor in eastern Indonesia in the same year, resulting in the death of approximately one hundred chickens from both commercial and backyard farms; however no evidence of disease has subsequently been reported in West Timor since 2007. A targeted survey was undertaken in 2013 in 2 districts of West Timor. Three hundred village and commercial poultry (292 chickens and 8 Muscovy ducks) from 10 villages and 5 live bird markets (LBMs) were sampled between August and October 2013...
February 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29391695/isolation-and-identification-of-a-cellulolytic-enterobacter-from-rumen-of-aceh-cattle
#9
Wenny Novita Sari, Safika, Darmawi, Yudha Fahrimal
Aims: The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes. Materials and Methods: Samples were collected from rumen fluid of Aceh's cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29383243/a-brodie-s-abscess-with-soft-tissue-collection-complicating-an-already-difficult-diagnosis
#10
Ben Gabbott, Giles Faria, George Lawson, Karen Daly
A healthy 14-year-old boy with a 3-month history of thigh pain and swelling is referred to a specialist centre with an uncertain diagnosis. After extensive tests and imaging a Brodie's abscess is diagnosed. The abscess is complicated by a fistula (cloaca) through the cortical bone of the femur, resulting in a large complex soft tissue collection. This is a presentation, we are unaware has been documented in any literature. After specialist multi-team combined management (Paediatric Trauma and Orthopaedic and Paediatric Infectious Diseases), the patient undergoes two surgical procedures, and extensive antibiotic cover...
January 2018: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/29378381/-antimicrobial-activity-of-ceftolozane-tazobactam-against-enterobacteriaceae-and-pseudomonas-aeruginosa-in-the-czech-republic-in-2016
#11
Helena Žemličková, Vladislav Jakubů, Marta Fridrichová
OBJECTIVE: Ceftolozane/tazobactam is an antibiotic effective against Gram-negative bacteria(including Pseudomonas aeruginosa).The study aimed at determining the effectiveness of the novel antibiotic in the Czech Republic. MATERIAL AND METHODS: The effectiveness of the antibiotic was studied in 16 Czech laboratories in 822 Enterobacteriaceae isolates (including AmpC and ESBL producers) and P. aeruginosa causing complicated intraabdominal or urinary tract infections...
December 2017: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/29371104/activity-of-ceftazidime-avibactam-against-carbapenemase-producing-enterobacteriaceae-from-urine-samples-obtained-during-the-infection-carbapenem-resistance-evaluation-surveillance-trial-icrest-in-spain
#12
María García-Castillo, Sergio García-Fernández, Rosa Gómez-Gil, Cristina Pitart, Marina Oviaño, Irene Gracia-Ahufinger, Jazmín Díaz-Regañón, Marta Tato, Rafael Cantón
The increasing rates of carbapenemase-producing Enterobacteriaceae (CPE) represent an important threat to health care systems and make treatment of CPE infections a challenge. The aim of the infection-carbapenem resistance evaluation surveillance trial (iCREST) was to determinate the prevalence of CPE in urine specimens in Spain and to evaluate the in vitro activity of ceftazidime-avibactam. Urine specimens (n=11,826) were included and activity of ceftazidime-avibactam and comparators were investigated by broth microdilution in CPE...
January 22, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29370894/continence-after-posterior-sagittal-anorectoplasty-for-anorectal-malformations-comparison-of-different-scores
#13
Giulia Brisighelli, Francesco Macchini, Dario Consonni, Antonio Di Cesare, Anna Morandi, Ernesto Leva
PURPOSE: To evaluate bowel function in patients with anorectal malformations (ARM) comparing existing scoring systems. METHODS: Parents of ARM patients treated at our institution were asked to fill in Holschneider, Kricknebeck, and Rintala questionnaires. Scores obtained from the questionnaires were expressed per cent and analyzed depending on the age and type of ARM according to Krickenbeck classification. Patients younger than 3 years of age or with developmental delay were excluded...
December 27, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29367928/dynamics-of-transgenic-enterobacter-cloacae-expressing-green-fluorescent-protein-defensin-gfp-d-in-anopheles-stephensi-under-laboratory-condition
#14
Hossein Dehghan, Mohammad Ali Oshaghi, Seyed Hassan Moosa-Kazemi, Bagher Yakhchali, Hassan Vatandoost, Naseh Maleki-Ravasan, Yavar Rassi, Habib Mohammadzadeh, Mohammad Reza Abai, Fatemeh Mohtarami
Background: Enterobacter cloacae bacterium is a known symbiont of the most Anopheles gut microflora and nominated as a good candidate for paratransgenic control of malaria. However, the population dynamics of this bacterium within An. stephensi and its introduction methods to the mosquitoes have not yet been explored. Methods: Enterobacter cloacae subsp. dissolvens expressing green fluorescent protein and defensin (GFP-D) was used to study transstadial transmission and the course of time, larval habitat, sugar, and blood meal on dynamics of the bacterium in the mosquito life stages in the laboratory condition...
December 2017: Journal of Arthropod-borne Diseases
https://www.readbyqxmd.com/read/29363955/synthesis-of-stachyobifiose-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#15
So Young Oh, So Youn Youn, Myeong Soo Park, Nam In Baek, Geun Eog Ji
The prebiotic effects of GOS (galactooligosaccharides) are known to depend on the glycosidic linkages, degree of polymerization (DP), and the monosaccharide composition. In this study, a novel form of α-GOS with a potentially improved prebiotic effect was synthesized using bifidobacterial α-galactosidase (α-Gal) purified from recombinant Escherichia coli. The carbohydrate produced was identified as α-d-galactopyranosyl-(1→6)-O-α-d-glucopyranosyl-(1→2)-[α-d-galactopyranosyl-(1→6)-O-β-d-fructofuranoside] and was termed stachyobifiose...
January 24, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29351668/evaluation-of-the-rapid-carbapenem-inactivation-method-rcim-a-phenotypic-screening-test-for-carbapenemase-producing-enterobacteriaceae
#16
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29349568/class-iii-bacteriocin-helveticin-m-causes-sublethal-damage-on-target-cells-through-impairment-of-cell-wall-and-membrane
#17
Zhilan Sun, Xiaomeng Wang, Xinxiao Zhang, Haihong Wu, Ye Zou, Pengpeng Li, Chong Sun, Weimin Xu, Fang Liu, Daoying Wang
Helveticin-M, a novel Class III bacteriocin produced by Lactobacillus crispatus exhibited an antimicrobial activity against Staphylococcus aureus, S. saprophyticus, and Enterobacter cloacae. To understand how Helveticin-M injured target cells, Helveticin-M was cloned and heterologously expressed in Escherichia coli. Subsequently, the cell wall organization and cell membrane integrity of target cells were determined. The mechanism of cellular damage differed according to bacterial species. Based on morphology analysis, Helveticin-M disrupted the cell wall of Gram-positive bacteria and disorganized the outer membrane of Gram-negative bacteria, therefore, altering surface structure...
January 18, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29344811/metabolic-evolution-and-a-comparative-omics-analysis-of-corynebacterium-glutamicum-for-putrescine-production
#18
Zhen Li, Yu-Ping Shen, Xuan-Long Jiang, Li-Shen Feng, Jian-Zhong Liu
Putrescine is widely used in the industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Because the highest titer of putrescine is much lower than that of its precursor L-ornithine reported in microorganisms to date, further work is needed to increase putrescine production in Corynebacterium glutamicum. We first compared 7 ornithine decarboxylase genes and found that the Enterobacter cloacae ornithine decarboxylase gene speC1 was most suitable for putrescine production in C. glutamicum...
January 17, 2018: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29343795/recurrent-wheezing-in-neonatal-pneumonia-is-associated-with-combined-infection-with-respiratory-syncytial-virus-and-staphylococcus-aureus-or-klebsiella-pneumoniae
#19
Qin Zhong, Hui Feng, Qi Lu, Xu Liu, Qi Zhao, Yue Du, Xian-Hong Zhang, Jia-Rong Wang
Both viral and bacterial infections can be associated with wheezing episodes in children; however, information regarding combined infections with both viral and bacterial pathogens in full term neonates is limited. We sought to investigate the effects of viral-bacterial codetection on pneumonia severity and recurrent wheezing. A retrospective cohort study was conducted on neonates admitted to our hospital with pneumonia from 2009 to 2015. Of 606 total cases, 341 were diagnosed with RSV only, and 265 were diagnosed with both RSV and a potential bacterial pathogen...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29342994/-report-of-antimicrobial-resistance-surveillance-program-in-chinese-children-in-2016
#20
C Q Wang, A M Wang, H Yu, H M Xu, C M Jing, J K Deng, R Z Zhao, C Z Hua, Y H Chen, X J Chen, T Zhang, H Zhang, Y P Chen, J G Yang, A W Lin, S F Wang, Q Cao, X Wang, H L Deng, S C Cao, J H He, W Gao, S Z Han
Objective: To analyze the antimicrobial resistance profile in Chinese children. Methods: This was a prevalence survey. From January 1 through December 31, 2016, the isolates were collected from 10 tertiary children hospitals in China. Antimicrobial susceptibility testing was carried out by routine laboratory methods. The penicillin susceptibility of streptococcus pneumonia and Meropenem susceptibility of gram-negative bacteria were detected by E-test and disk diffusion method respectively. Antimicrobial susceptibility results were interpreted according to the criteria of Clinical and Laboratory Standards Institute (CLSI) Guideline 2016...
January 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
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