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https://www.readbyqxmd.com/read/28721477/imipenem-resistant-gram-negative-bacterial-isolates-carried-by-persons-upon-medical-examination-in-korea
#1
So Yeon Kim, Sang Yop Shin, Ji-Young Rhee, Kwan Soo Ko
Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method...
July 18, 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28720316/pigeons-and-their-droppings-as-reservoirs-of-candida-and-other-zoonotic-yeasts
#2
Inmaculada Rosario Medina, Lorena Román Fuentes, Miguel Batista Arteaga, Fernando Real Valcárcel, Félix Acosta Arbelo, Daniel Padilla Del Castillo, Soraya Déniz Suárez, Otilia Ferrer Quintana, Belinda Vega Gutiérrez, Freddy Silva Sergent, Begoña Acosta-Hernández
BACKGROUND: The importance of pigeons as reservoirs and carriers of Cryptococcus neoformans and other species of this genus is well-known; however, less is known about their role as reservoirs and carriers of other yeasts that impact public health. AIMS: The present study was performed on Gran Canaria Island to define yeasts other than Cryptococcus spp. that have been reported to impact public health and which could be carried by pigeons. METHODS: Samples were obtained from 83 pigeon lofts (Columba livia); moreover, 331 crop samples, 331 cloacal samples and 174 dropping samples were collected...
July 15, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28713580/severe-hypothermia-in-transported-pullets-case-study-of-its-occurrence-diagnosis-and-treatment-using-active-external-rewarming-technique
#3
Ndazo S Minka, Joseph O Ayo
Sixty pullets, aged 17 weeks, were presented when presumed to be 'dead' after being exposed to wet-cold weather transportation. The birds appeared unconscious and their feathers were soaking wet, and with a body rigid and cold to touch. The aim of the study was to resuscitate the hypothermic pullets. Blood samples were obtained and core body temperature recorded before and after rewarming. The birds were resuscitated using active external rewarming technique. Blood samples revealed significant (P < 0...
May 2017: Veterinary Medicine and Science
https://www.readbyqxmd.com/read/28712037/introduction-of-sample-tubes-with-sodium-azide-as-a-preservative-for-ethyl-glucuronide-in-urine
#4
Marc Luginbühl, Wolfgang Weinmann, Ali Al-Ahmad
Ethyl glucuronide (EtG) is a direct alcohol marker, which is widely used for clinical and forensic applications, mainly for abstinence control. However, the instability of EtG in urine against bacterial degradation or the post-collectional synthesis of EtG in contaminated samples may cause false interpretation of EtG results in urine samples. This study evaluates the potential of sodium azide in tubes used for urine collection to hinder degradation of ethyl glucuronide by bacterial metabolism taking place during growth of bacterial colonies...
July 15, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28708518/ultrasonography-to-assist-with-timing-of-spawning-in-european-eel
#5
Pauline Jéhannet, Leon T N Heinsbroek, Arjan P Palstra
Ovulation in the European eel can be induced by injection of DHP (17α,20β-dihydroxy-4-pregnen-3-one). The timing of injection is based on the developmental stage of the oocytes. The oocyte stage is determined by invasive biopsy or through external indicators of the oocyte hydration response: body weight index (BWI) and body girth index (BGI). However, in European eel, BWI and BGI are inaccurate indicators because the individual hydration response is highly variable. The aim of this study was to identify indicators of oocyte maturation non-invasively by applying ultrasonography...
June 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28707775/giant-fetal-hydrometrocolpos-associated-with-cloacal-anomaly-causing-postnatal-respiratory-distress
#6
Tatsuhito Kanda, Takashi Iizuka, Rena Yamazaki, Junpei Iwadare, Masanori Ono, Hiroshi Fujiwara
Persistent cloaca is a rare presentation wherein the urethra, vagina, and rectum converge into a common channel with a single perineal opening. Fetal hydrometrocolpos can result if fluid accumulates behind an obstruction of this common channel. A 29-year-old woman (G4P1021) was referred at 36 2/7 weeks of gestation for evaluation of a fetal abdominal cystic mass. Detailed ultrasonography and magnetic resonance imaging showed two symmetric cystic masses, bilateral hydronephrosis, and oligohydramnios. Elective cesarean delivery was performed at 37 0/7 weeks; the baby weighed 4043 g with Apgar scores of 5 and 6 at 1 and 5 min...
July 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28702005/insights-into-a-novel-blakpc-2-encoding-incp-6-plasmid-reveal-carbapenem-resistance-circulation-in-several-enterobacteriaceae-species-from-wastewater-and-a-hospital-source-in-spain
#7
Yancheng Yao, Fernando Lazaro-Perona, Linda Falgenhauer, Aránzazu Valverde, Can Imirzalioglu, Lucas Dominguez, Rafael Cantón, Jesús Mingorance, Trinad Chakraborty
Untreated wastewater, particularly from hospitals and other healthcare facilities, is considered to be a reservoir for multidrug-resistant bacteria. However, its role in the spread of antibiotic resistances in the human population remains poorly investigated. We used whole genome sequencing to analyze 25 KPC-2-producing Enterobacteriaceae isolates from sewage water collected during a 3-year period and three clinical Citrobacter freundii isolates from a tertiary hospital in the same collection area in Spain...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28698918/micropleura-huchzermeyeri-n-sp-camallanida-dracunculoidea-micropleuridae-from-the-nile-crocodile-crocodylus-niloticus-laurenti-reptilia-crocodylidae-in-south-africa
#8
Kerstin Junker, Yasen Mutafchiev
Micropleura huchzermeyeri n. sp. (Camallanida: Dracunculoidea: Micropleuridae) is described from the peritoneal cavity of Crocodylus niloticus Laurenti (Reptilia: Crocodylidae), based on a detailed study of its morphology using light and scanning electron microscopy. The new species is compared with its congeners, M. australiensis Moravec, Kay & Hobbs, 2004, M. vazi Travassos, 1933 and M. vivipara von Linstow, 1906 from crocodilians, and with M. indica Khera, 1951 from chelonian hosts. It can be distinguished from these by the length of its spicules, which are longer than in all described species and a combination of characters, including the presence of prominent lateral caudal papillae on the level of the cloaca in males, the presence of conspicuous phasmids on the female tail, the pre-equatorial position of the vulva and the length of the first-stage larvae...
July 11, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28698589/transglycosylation-by-a-chitinase-from-enterobacter-cloacae-subsp-cloacae-generates-longer-chitin-oligosaccharides
#9
Mohan Krishna Mallakuntla, Papa Rao Vaikuntapu, Bhoopal Bhuvanachandra, Subha Narayan Das, Appa Rao Podile
Humans have exploited natural resources for a variety of applications. Chitin and its derivative chitin oligosaccharides (CHOS) have potential biomedical and agricultural applications. Availability of CHOS with the desired length has been a major limitation in the optimum use of such natural resources. Here, we report a single domain hyper-transglycosylating chitinase, which generates longer CHOS, from Enterobacter cloacae subsp. cloacae 13047 (EcChi1). EcChi1 was optimally active at pH 5.0 and 40 °C with a Km of 15...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28698036/surgical-site-infection-with-extended-spectrum-beta-lactamase-esbl-producing-enterobacteriaceae-after-cardiac-surgery-incidence-and-risk-factors
#10
Sarah Jolivet, Francois-Xavier Lescure, Laurence Armand-Lefevre, Richard Raffoul, Marie-Pierre Dilly, Walid Ghodbane, Patrick Nataf, Jean-Christophe Lucet
OBJECTIVE: The aims of this study were to determine the incidence, microbiology and risk factors for sternal wound infection (SWI) with extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) following cardiac surgery. METHODS: We performed a retrospective analysis between January 2006 and December 2015 of prospective surveillance of a cohort of patients with cardiac surgery at a single centre (Paris, France). SWI was defined as the need for reoperation due to sternal infection...
July 8, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28696788/investigation-of-medium-chain-fatty-acid-feed-supplementation-for-reducing-salmonella-typhimurium-colonization-in-turkey-poults
#11
Nicholas P Evans, David A Collins, Frank William Pierson, Hassan M Mahsoub, Nammalwar Sriranganathan, Mike E Persia, Theodore Peter Karnezos, Michael D Sims, Rami A Dalloul
Studies indicate that persistent Salmonella colonization occurs in poultry that are infected early in life, leading to both food safety and public health concerns. Development of improved preharvest Salmonella management strategies is needed to reduce poultry product contamination. The objective of this study was to evaluate the efficacy of a product containing medium chain fatty acids (MCFA) for reducing early Salmonella colonization in turkey poults. Day-of-hatch turkeys were provided a standard starter diet supplemented with MCFA at 0 (negative and positive controls), 1...
July 11, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28691244/probiotics-intake-from-proximal-or-distal-gastrointestinal-tract-the-investigation-on-intestinal-morphology-and-performance-of-japanese-quail
#12
K Seifi, M A Karimi-Torshizi, H Deldar
The aim of the present study was to investigate the efficacy of early single-dose probiotics administered from proximal or distal gastrointestinal tract on quails' performance and small intestinal morphology. A total of 120 Japanese quail (Coturnix coturnix japonica) 1-d-old chicks were divided into the following experimental groups before being transferred to the raising room: (i) control (no probiotic administered); (ii) oral group (received probiotics via oesophageal gavage); and (iii) vent lip group (received probiotics directly into vent)...
July 10, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/28690591/characterization-of-the-microbiome-along-the-gastrointestinal-tract-of-growing-turkeys
#13
Toby J Wilkinson, A A Cowan, H E Vallin, L A Onime, Linda B Oyama, S J Cameron, Charlotte Gonot, J M Moorby, K Waddams, V J Theobald, D Leemans, S Bowra, C Nixey, Sharon A Huws
The turkey microbiome is largely understudied, despite its relationship with bird health and growth, and the prevalence of human pathogens such as Campylobacter spp. In this study we investigated the microbiome within the small intestine (SI), caeca (C), large intestine (LI), and cloaca (CL) of turkeys at 6, 10, and 16 weeks of age. Eight turkeys were dissected within each age category and the contents of the SI, C, LI, and CL were harvested. 16S rDNA based QPCR was performed on all samples and samples for the four locations within three birds/age group were sequenced using ion torrent-based sequencing of the 16S rDNA...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28689800/investigation-and-analysis-of-the-characteristics-and-drug-sensitivity-of-bacteria-in-skin-ulcer-infections
#14
Hang Yang, Wen-Sheng Wang, Yang Tan, Dao-Jun Zhang, Jin-Jin Wu, Xia Lei
PURPOSE: Skin ulcer is a common type of disease affecting patients' health and quality of life, and bacterial infection increases the difficulty of its management. METHODS: The present study collected the results of bacterial culture sampled from the surface of 110 cases of skin ulcers at our hospital from January 2011 to December 2012. We analyzed the constituent ratios of ulcer surface bacteria, the change in the main infectious bacteria and the results of drug-sensitivity testing for common bacteria...
May 24, 2017: Chinese Journal of Traumatology, Zhonghua Chuang Shang za Zhi
https://www.readbyqxmd.com/read/28678838/the-oral-bacterial-microbiome-of-occlusal-surfaces-in-children-and-its-association-with-diet-and-caries
#15
Apoena Aguiar Ribeiro, Maria Andrea Azcarate-Peril, Maria Belen Cadenas, Natasha Butz, Bruce J Paster, Tsute Chen, Eric Bair, Roland R Arnold
Dental caries is the most prevalent disease in humans globally. Efforts to control it have been invigorated by an increasing knowledge of the oral microbiome composition. This study aimed to evaluate the bacterial diversity in occlusal biofilms and its relationship with clinical surface diagnosis and dietary habits. Anamneses were recorded from thirteen 12-year-old children. Biofilm samples collected from occlusal surfaces of 46 permanent second molars were analyzed by 16S rRNA amplicon sequencing combined with the BLASTN-based search algorithm for species identification...
2017: PloS One
https://www.readbyqxmd.com/read/28676058/chemical-constituents-antibacterial-and-antioxidant-properties-of-the-essential-oil-flower-of-tagetes-minuta-grown-in-cala-community-eastern-cape-south-africa
#16
Aboi Igwaran, Benson Chucks Iweriebor, Sunday Ofuzim Okoh, Uchechukwu Uchechukwu Nwodo, Larry Chikwelu Obi, Anthony Ifeanyi Okoh
BACKGROUND: Tagetes minuta has a long record of human use for the treatment of stomach and intestinal diseases. Most drugs used for diseases treatment are less efficacious with side effects and this brought the search for new treatment regimens mainly from medicinal plants. METHOD: The essential oil (EO) was extracted by Clevenger's-type apparatus and its chemical composition, antioxidant and antibacterial properties were determined by GC-MS, spectrophotometric and broth dilution methods respectively...
July 5, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28673795/use-of-3d-reconstruction-cloacagrams-and-3d-printing-in-cloacal-malformations
#17
Jennifer J Ahn, Margarett Shnorhavorian, Anne-Marie E Amies Oelschlager, Beth Ripley, Giridhar M Shivaram, Jeffrey R Avansino, Paul A Merguerian
INTRODUCTION: Cloacal anomalies are complex to manage, and the anatomy affects prognosis and management. Assessment historically includes examination under anesthesia, and genitography is often performed, but these do not consistently capture three-dimensional (3D) detail or spatial relationships of the anatomic structures. Three-dimensional reconstruction cloacagrams can provide a high level of detail including channel measurements and the level of the cloaca (<3 cm vs. >3 cm), which typically determines the approach for surgical reconstruction and can impact long-term prognosis...
June 20, 2017: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28671537/the-distribution-of-carbapenem-and-colistin-resistance-in-gram-negative-bacteria-from-the-tamil-nadu-region-in-india
#18
Prasanth Manohar, Thamaraiselvan Shanthini, Ramankannan Ayyanar, Bulent Bozdogan, Aruni Wilson, Ashok J Tamhankar, Ramesh Nachimuthu, Bruno S Lopes
PURPOSE: The occurrence of carbapenem- and colistin-resistance among Gram-negative bacteria is increasing worldwide. The aim of this study was to understand the distribution of carbapenem- and colistin-resistance in two areas in Tamil Nadu, India. METHODOLOGY: The clinical isolates (n=89) used in this study were collected from two diagnostic centres in Tamil Nadu, India. The bacterial isolates were screened for meropenem- and colistin-resistance. Further, resistance genes blaNDM-1, blaOXA-48-like, blaIMP, blaVIM, blaKPC, mcr-1 and mcr-2 and integrons were studied...
July 3, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28670411/polymicrobial-and-monomicrobial-infections-after-spinal-surgery-a-retrospective-study-to-determine-which-infection-is-more-severe
#19
Shaoqiang Liu, Qiang Qi, Zhongqiang Chen, Ning Liu, Zhaoqing Guo, Chuiguo Sun, Weishi Li, Yan Zeng, Zhongjun Liu
STUDY DESIGN: A retrospective clinical review. PURPOSE: To investigate the difference in clinical manifestations and severity between polymicrobial and monomicrobial infections after spinal surgery. OVERVIEW OF LITERATURE: Surgical site infections (SSIs) after spinal surgery are a major diagnostic and therapeutic challenge for spinal surgeons. Polymicrobial infections after spinal surgery seem to result in poorer outcomes than monomicrobial infections because of complementary resistance to antibiotics...
June 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/28659317/whole-genome-sequencing-overrules-a-suspected-case-of-carbapenem-resistant-enterobacter-cloacae-transmission
#20
Mark Chen, Sean Conlan, Anna F Lau, John P Dekker, Clay Deming, David K Henderson, Karen M Frank, Tara N Palmore, Julia A Segre
Enterobacter cloacae is an important nosocomial bacteria that is prevalent in hospital intensive care units (ICUs) (1-4).….
June 28, 2017: Journal of Clinical Microbiology
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