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https://www.readbyqxmd.com/read/28521744/determination-of-the-sources-and-antimicrobial-resistance-patterns-of-salmonella-isolated-from-the-poultry-industry-in-southern-ethiopia
#1
Reta Duguma Abdi, Fisseha Mengstie, Ashenafi Feyisa Beyi, Takele Beyene, Hika Waktole, Bedasso Mammo, Dinka Ayana, Fufa Abunna
BACKGROUND: Ethiopia set an ambitious masterplan to increase chicken meat and egg production from 2015 to 2020. Poultry breeding, multiplication and distribution centers in the country have received executive order to import, amplify and distribute commercial chickens to end users. The biosecurity and the pathogen fauna of the centers have not been evaluated as to whether the centers could implement the mission effectively without any risk. Thus, the aim of this study was to evaluate the biosecurity practices and the pathogen prevalence, risk factors and their antimicrobial resistance (AMR) using Salmonella as case study...
May 18, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28520983/genomic-epidemiology-of-global-vim-producing-enterobacteriaceae
#2
Yasufumi Matsumura, Gisele Peirano, Rebekah Devinney, Patricia A Bradford, Mary R Motyl, Mark D Adams, Liang Chen, Barry Kreiswirth, Johann D D Pitout
Background: : International data on the molecular epidemiology of Enterobacteriaceae with VIM carbapenemases are limited. Methods: We performed short read (Illumina) WGS on a global collection of 89 VIM-producing clinical Enterobacteriaceae (2008-14). Results: VIM-producing (11 varieties within 21 different integrons) isolates were mostly obtained from Europe. Certain integrons with bla VIM were specific to a country in different species and clonal complexes (CCs) (In 87 , In 624 , In 916 and In 1323 ), while others had spread globally among various Enterobacteriaceae species (In 110 and In 1209 )...
May 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28509939/bioremoval-of-lead-using-pennisetum-purpureum-augmented-with-enterobacter-cloacae-vitpasj1-a-pot-culture-approach
#3
Anamika Das, Priyanka Belgaonkar, Aditya S Raman, Sofia Banu, Jabez W Osborne
Lead is a toxic heavy metal discharged into the ecosystem from various industries. Biological remediation strategies have been effective in the bioremoval of lead. In our current study, a phytobacterial system using Pennisetum purpureum along with lead-resistant bacterium (LRB) was employed for the uptake of lead. The LRB was obtained from lead-contaminated sites. The isolate VITPASJ1 was found to be highly tolerant to lead and was identified as an effective plant growth-promoting bacterium. The 16S rRNA sequencing revealed VITPASJ1 to be the closest neighbour of Enterobacter cloacae...
May 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28508092/a-metabonomic-analysis-on-the-response-of-enterobacter-cloacae-from-coastal-outfall-for-land-based-pollutant-under-phoxim-stress
#4
Dijun Zhang, Shan He, Tinghong Ming, Chenyang Lu, Jun Zhou, Xiurong Su
Enterobacter cloacae is an opportunistic pathogen widely distributed in human and animal intestinal systems. The secretion of extended-spectrum β-lactamases (ESBLs) and cephalosporinase (AmpC) endows E. cloacae with strong drug resistance. In a previous study by our group, protein expression of E. cloacae under phoxim stress was measured by two-dimensional electrophoresis. Here, nuclear magnetic resonance was used to detect differences in E. cloacae metabonomics when under phoxim stress. We determined that there are 29 types of metabolites that differ between phoxim stress and normal culture conditions...
May 15, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28507488/microbiological-analysis-of-acute-infections-of-the-nail-fold-on-the-basis-of-bait-thread-test
#5
Hanna Tomczak, Aleksandra Dańczak-Pazdrowska, Adriana Polańska, Agnieszka Osmola-Mańkowska, Jakub Pazdrowski, Wioleta Błażejewska-Gąsior, Anna Horla, Marta Hasse-Cieślińska, Zygmunt Adamski
INTRODUCTION: An acute infection of the nail fold, called paronychia, is a common clinical problem. The basis for the implementation of the treatment is the result of microbiological examination. Due to the rapid and painful course of infection, usually an empirical antimicrobial treatment prior to obtaining microbiological test results is introduced. AIM: The microbial analysis of acute infections of the nail fold. MATERIAL AND METHODS: The study included 32 tests conducted on 31 patients of the Department of Dermatology...
April 2017: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/28493980/characterization-of-the-juvenile-green-turtle-chelonia-mydas-microbiome-throughout-an-ontogenetic-shift-from-pelagic-to-neritic-habitats
#6
James T Price, Frank V Paladino, Margaret M Lamont, Blair E Witherington, Scott T Bates, Tanya Soule
The gut microbiome of herbivorous animals consists of organisms that efficiently digest the structural carbohydrates of ingested plant material. Green turtles (Chelonia mydas) provide an interesting model of change in these microbial communities because they undergo a pronounced shift from a surface-pelagic distribution and omnivorous diet to a neritic distribution and herbivorous diet. As an alternative to direct sampling of the gut, we investigated the cloacal microbiomes of juvenile green turtles before and after recruitment to neritic waters to observe any changes in their microbial community structure...
2017: PloS One
https://www.readbyqxmd.com/read/28491136/applications-of-microalgal-biofilms-for-wastewater-treatment-and-bioenergy-production
#7
Ana F Miranda, Narasimhan Ramkumar, Constandino Andriotis, Thorben Höltkemeier, Aneela Yasmin, Simone Rochfort, Donald Wlodkowic, Paul Morrison, Felicity Roddick, German Spangenberg, Banwari Lal, Sanjukta Subudhi, Aidyn Mouradov
BACKGROUND: Microalgae have shown clear advantages for the production of biofuels compared with energy crops. Apart from their high growth rates and substantial lipid/triacylglycerol yields, microalgae can grow in wastewaters (animal, municipal and mining wastewaters) efficiently removing their primary nutrients (C, N, and P), heavy metals and micropollutants, and they do not compete with crops for arable lands. However, fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30-40% of the total cost of biodiesel production...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28491055/metagenomic-sequencing-of-diamondback-moth-gut-microbiome-unveils-key-holobiont-adaptations-for-herbivory
#8
Xiaofeng Xia, Geoff M Gurr, Liette Vasseur, Dandan Zheng, Huanzi Zhong, Bingcai Qin, Junhan Lin, Yue Wang, FengQin Song, Yong Li, Hailan Lin, Minsheng You
Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28485041/editorial-comment-to-a-unique-defect-persistent-posterior-cloaca-an-example-of-staged-genito-urinary-and-digestive-tract-reconstruction-with-an-alternative-vaginal-creation-using-the-urinary-bladder-wall
#9
Kazuyoshi Johnin
No abstract text is available yet for this article.
May 8, 2017: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/28476242/evaluation-of-the-carbapenem-inactivation-method-cim-for-detecting-carbapenemase-activity-in-enterobacteria
#10
A Aguirre-Quiñonero, M E Cano, D Gamal, J Calvo, L Martínez-Martínez
OBJECTIVES: The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. METHODS: A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation...
March 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28475792/clinical-and-molecular-epidemiology-of-carbapenem-resistant-enterobacteriaceae-among-adult-inpatients-in-singapore
#11
Kalisvar Marimuthu, Indumathi Venkatachalam, Wei Xin Khong, Tse Hsien Koh, Benjamin Pei Zhi Cherng, My Van La, Partha Pratim De, Prabha Unny Krishnan, Thean Yen Tan, Raymond Fong Kok Choon, Surinder Kaur Pada, Choong Weng Lam, Say Tat Ooi, Rama Narayana Deepak, Nares Smitasin, Eng Lee Tan, Jia Jun Lee, Asok Kurup, Barnaby Young, Nancy Tee Wen Sim, Koh Cheng Thoon, Dale Fisher, Moi Lin Ling, Brenda Ang Sze Peng, Yik-Ying Teo, Li Yang Hsu, Raymond Tzer Pin Lin, Rick Twee-Hee Ong, Jeanette Teo, Oon Tek Ng
Background: Since 2010, the incidence of carbapenem-resistant Enterobacteriaceae (CRE) has been increasing in Singapore. We analyzed the clinical and molecular epidemiology of CRE among adult inpatients in Singapore. Methods: Quarterly incidence of unique subjects (per 100000 patient-days) with positive clinical and surveillance cultures for CRE were estimated based on mandatory data submitted to the National Public Health Laboratory by public hospitals between 2010 and 2015...
May 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28473892/characterization-of-isolated-pigeon-paramyxovirus-1-pmv-1-and-its-pathogenicity-in-broiler-chickens
#12
Mansour Mayahi, Masoud Reza Seyfi Abad Shapouri, Ramezan Ali Jafari, Mehrdad Khosravi Farsani
Characterization of isolated pigeon paramyxovirus-1 (PMV-1) and its pathogenicity in broiler chickens were studied. Two hundred and thirty-two samples collected from 50 unvaccinated pigeons lofts suspected to Newcastle disease from private houses and bird markets from Ahvaz, Iran. Swab samples from cloaca and oropharynx of live pigeons and from trachea, lung, liver, spleen, kidney, brain, proventriculus and cecal tonsil of dead pigeons suspected to ND were collected. Isolation of the PPMV-1 was performed through intra-allantoic inoculation of 9- to 11- day-old embryonated chicken eggs...
2017: Veterinary Research Forum
https://www.readbyqxmd.com/read/28472451/effects-of-embryo-temperature-estimation-methodology-on-the-determination-of-eggshell-conductance-values-in-ross-708-broiler-hatching-eggs-with-consideration-given-to-eggshell-pigmentation-variation1-2-3
#13
O C Olojede, S D Filip To, C D McDaniel, E D Peebles
In a preliminary experiment, the influence of pigment color variation on the temperature readings of the shells (EST) of live embryonated Ross 708 broiler hatching eggs was tested. Prior to set, eggshell color in L*, a*, and b* coordinates were measured at the equator of each egg. Eggshell pigmentation was found not to influence EST from zero through 18 d of incubation (DOI). In a subsequent experiment, EST, as well as air cell (ACT) and cloaca (CLT) temperature measurements were used for the calculation of absolute (GH2O) and specific (gH20) eggshell conductance values for these same types of eggs...
May 3, 2017: Poultry Science
https://www.readbyqxmd.com/read/28466047/a-sensitive-haemadsorption-technique-based-rt-pcr-for-concentration-and-detection-of-newcastle-disease-virus-from-clinical-samples-and-allantoic-fluid
#14
Perumal Arumugam Desingu, Shambhu Dayal Singh, Kuldeep Dhama, Obli Rajendran Vinodhkumar, Yashpal Singh Malik
The present study describes the exploitation of haemadsorption (HAd) property of the Newcastle disease virus (NDV) for the development of a novel sensitive HAd technique based RT-PCR for detection of NDV from clinical samples of virus infected experimental birds. The NDV propagated allantoic fluid from the infected embryonated chicken eggs or supernatant of the processed clinical samples (tissue triturate, cloaca and tracheal swabs) from the experimentally infected birds were added with chicken red blood cells (RBC) to adsorb the virus on RBC's surface...
September 2016: Virusdisease
https://www.readbyqxmd.com/read/28458566/usefulness-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-to-identify-pathogens-including-polymicrobial-samples-directly-from-blood-culture-broths
#15
Maya Hariu, Yuji Watanabe, Nozomi Oikawa, Masafumi Seki
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (TOF-MS) is now widely used to detect pathogens in clinical settings in Japan. Here, we report the ability of TOF-MS to detect bacteria from blood culture (BC) broths, and compare the efficacy of TOF-MS to that of conventional culture methods. Bacteria were correctly detected from 63 monomicrobial samples within 80 minutes; results matched those obtained by conventional BC methods, although the conventional methods took 2-3 days. In addition to the 63 monomicrobial samples, another three polymicrobial samples were tested; notably, the infecting bacteria were not correctly identified in two of these three samples...
2017: Infection and Drug Resistance
https://www.readbyqxmd.com/read/28449650/structure-genetics-and-worldwide-spread-of-new-delhi-metallo-%C3%AE-lactamase-ndm-a-threat-to-public-health
#16
REVIEW
Asad U Khan, Lubna Maryam, Raffaele Zarrilli
BACKGROUND: The emergence of carbapenemase producing bacteria, especially New Delhi metallo-β-lactamase (NDM-1) and its variants, worldwide, has raised amajor public health concern. NDM-1 hydrolyzes a wide range of β-lactam antibiotics, including carbapenems, which are the last resort of antibiotics for the treatment of infections caused by resistant strain of bacteria. MAIN BODY: In this review, we have discussed bla NDM-1variants, its genetic analysis including type of specific mutation, origin of country and spread among several type of bacterial species...
April 27, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28442305/transfer-of-kpc-2-carbapenemase-from-klebsiella-pneumoniae-to-enterobacter-cloacae-in-a-patient-receiving-meropenem-therapy
#17
Evelin Rodrigues Martins, Cássia Fernanda Estofolete, Andressa Batista Zequini, Louise Cerdeira, Doroti de Oliveira Garcia, Maria Fernanda Campagnari Bueno, Gabriela Rodrigues Francisco, Leonardo Neves de Andrade, Ana Lúcia da Costa Darini, Fernanda Modesto Tolentino, Tiago Casella, Nilton Lincopan, Mara Corrêa Lelles Nogueira
The horizontal transfer of a plasmid bearing the blaKPC-2 gene from K. pneumoniae to E. cloacae infecting the respiratory tract of a patient during meropenem therapy was elucidated. This finding is particularly worrisome, since these drugs are of last resort for multidrug-resistant Gram-negative pathogens.
April 12, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28434982/novel-monoclonal-antibodies-against-stx1d-and-1e-and-their-use-for-improving-immunoassays
#18
Xiaohua He, Stephanie Patfield, Reuven Rasooly, Daniela Mavrici
Shiga toxins (Stxs) are major causative agents for bloody diarrhea and hemolytic uremic syndrome, a life-threatening disease in humans. No effective treatment is available. Early detection of Stxs in clinical samples is critical for disease management. As bacteria evolve, new Stxs are produced; therefore, methods used to identify them need to be improved as well. In this study, new monoclonal antibodies (mAbs) against Stx1d and 1e were developed and used to improve a commercial Stx1 kit. Incorporation of the new mAbs into the Abraxis Stx1 kit not only increased the assay sensitivity to Stx1d, but the assay was conferred the ability to detect Stx1e, a newly identified subtype of Stx1 produced by an atypical Stx-producing bacterial strain, Enterobacter cloacae M12X01451, isolated from a clinical specimen...
April 20, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28428984/a-comparison-of-molecular-typing-methods-applied-to-enterobacter-cloacae-complex-hsp60-sequencing-rep-pcr-and-mlst
#19
Roberto A Viau, Lee M Kiedrowski, Barry N Kreiswirth, Mark Adams, Federico Perez, Dror Marchaim, Dubert M Guerrero, Keith S Kaye, Latania K Logan, Maria Virginia Villegas, Robert A Bonomo
Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST)...
2017: Pathogens & Immunity
https://www.readbyqxmd.com/read/28425035/a-new-ascaridid-nematode-mammalakis-zambiensis-n-sp-heterakoidea-kiwinematidae-from-the-mole-rat-fukomys-anselli-burda-zima-scharff-machol%C3%A3-n-kawalika-rodentia-bathyergidae-in-zambia
#20
Kerstin Junker, Heike Lutermann, Yasen Mutafchiev
Mammalakis zambiensis n. sp. is described from the large intestine and caecum of Fukomys anselli (Burda, Zima, Scharff, Macholán & Kawalika), a bathyergid rodent endemic to Zambia. Morphological data for comparison with its three congeners, M. macrospiculum (Ortlepp, 1939), M. spalacis (Marcu, 1930) and M. spalaxi (Kozlov & Jangolenko, 1962) are provided. Based on studies of the type-material, observations of hitherto undescribed morphological characters of M. macrospiculum, a parasite of Bathyergus suillus (Schreber) (Hystricomorpha: Bathyergidae) in South Africa, are presented...
April 19, 2017: Systematic Parasitology
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