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peptides and tuberculosis

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https://www.readbyqxmd.com/read/29321721/antigen-85b-peptidomic-analysis-allows-species-specific-mycobacterial-identification
#1
Wei Zhang, Qingbo Shu, Zhen Zhao, Jia Fan, Christopher J Lyon, Adrian M Zelazny, Ye Hu
Background: Nontuberculous mycobacteria (NTM)-mediated infections are a growing cause of worldwide morbidity, but lack of rapid diagnostics for specific NTM species can delay the initiation of appropriate treatment regimens. We thus examined whether mass spectrometry analysis of an abundantly secreted mycobacterial antigen could identify specific NTM species. Methods: We analyzed predicted tryptic peptides of the major mycobacterial antigen Ag85B for their capacity to distinguish Mycobacterium tuberculosis and three NTM species responsible for the majority of pulmonary infections caused by slow-growing mycobacterial species...
2018: Clinical Proteomics
https://www.readbyqxmd.com/read/29306028/unique-mtb-protein-found-in-urine-of-tb-patients-discovery-of-a-unique-mycobacterium-tuberculosis-protein-through-proteomic-analysis-of-urine-from-patients-with-active-tuberculosis
#2
Nira Pollock, Rakesh Dhiman, Nada Daifalla, Maha Farhat, Antonio Campos-Neto
Identification of pathogen-specific biomarkers present in patients' serum or urine samples can be a useful diagnostic approach. In efforts to discover Mycobacterium tuberculosis (Mtb) biomarkers we identified by mass spectroscopy a unique 21-mer Mtb peptide sequence (VVLGLTVPGGVELLPGVALPR) present in the urines of TB patients from Zimbabwe. This peptide has 100% sequence homology with the protein TBCG_03312 from the C strain of Mtb (a clinical isolate identified in New York, NY, USA) and 95% sequence homology with Mtb oxidoreductase (MRGA423_21210) from the clinical isolate MTB423 (identified in Kerala, India)...
January 3, 2018: Microbes and Infection
https://www.readbyqxmd.com/read/29209209/nonribosomal-peptides-from-marine-microbes-and-their-antimicrobial-and-anticancer-potential
#3
REVIEW
Shivankar Agrawal, Debabrata Acharya, Alok Adholeya, Colin J Barrow, Sunil K Deshmukh
Marine environments are largely unexplored and can be a source of new molecules for the treatment of many diseases such as malaria, cancer, tuberculosis, HIV etc. The Marine environment is one of the untapped bioresource of getting pharmacologically active nonribosomal peptides (NRPs). Bioprospecting of marine microbes have achieved many remarkable milestones in pharmaceutics. Till date, more than 50% of drugs which are in clinical use belong to the nonribosomal peptide or mixed polyketide-nonribosomal peptide families of natural products isolated from marine bacteria, cyanobacteria and fungi...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29207705/serum-vitamin-d-level-as-a-risk-factor-for-female-genital-tuberculosis-fgtb
#4
Swati Gautam, Amita Jain, Salman Akhtar, Apala Priyadarshini, Shyam Pyari Jaiswar
Introduction: Vitamin D is now known to be essential to Mycobacterium tuberculosis containment and killing through activation of 25-hydroxyvitamin-D receptors (VDRs) present on all immune cells or obtained from dietary food stuffs as either vitamin D3 or vegetable vitamin D2 (also known as ergocalciferol). Aim: To evaluate the association of serum vitamin D level between the Female Genital Tuberculosis (FGTB) cases and healthy controls. Materials and Methods: Total 120 cases and 120 controls enrolled for the study following inclusion and exclusion criteria...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29180322/-peripheral-blood-t-cell-tnf-%C3%AE-and-ifn-%C3%AE-production-stimulated-by-low-molecular-peptide-of-mycobacterium-tuberculosis-heat-resistant-antigen-for-differential-diagnosis-between-pulmonary-tuberculosis-and-latent-tuberculosis-infection
#5
Jie Tang, Ce Chen, Cheng Zha, Jian-Rong Chang, Qiang Fang, Zhao-Hua Wang, Bai-Qing Li
OBJECTIVE: To investigate the effects of low molecular peptide of Mycobacterium tuberculosis heat-resistant antigen (Mtb-HAg-10k) on the production of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood T cells and test the feasibility of differential diagnosis between pulmonary tuberculosis (PTB) and latent tuberculosis infection (LTBI) by assessing the number of Mtb-HAg-10k-stimulated IFN-γ-producing T cells. METHODS: Peripheral blood mononuclear cells (PBMCs) were separated from the peripheral blood of 10 healthy adults, 6 individuals with LTBI and 13 patients with PTB...
November 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29178041/cellular-immune-response-in-mdr-tb-patients-to-different-protein-expression-of-mdr-and-susceptible-mycobacterium-tuberculosis-rv0147-a-novel-mdr-tb-biomarker
#6
Alireza Hadizadeh Tasbiti, Shamsi Yari, Seyed Davar Siadat, Payam Tabarsi, Kayvan Saeedfar, Fatemeh Yari
Tuberculosis (TB) is a crucial public health problem with prevalence of multidrug resistant (MDR) rising. An accurate TB biomarker is urgently needed to monitor the response to treatment in patients with MDR tuberculosis. To analyze interaction between selected MDR-TB purified protein and immune cells, dendritic cells from MDR-TB patients and healthy subjects were stimulated by 55KDa protein fractions (Rv0147). The purified proteins identified by proteomic techniques (two-dimensional gel electrophoresis, mass spectrometry) and peptide sequences are known to bind a MHC class I alleles which are extracted from the Immune Epitope Database and Analysis Resource database ( www...
November 24, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29176828/t-cell-recognition-of-mycobacterium-tuberculosis-peptides-presented-by-hla-e-derived-from-infected-human-cells
#7
Curtis McMurtrey, Melanie J Harriff, Gwendolyn M Swarbrick, Amanda Duncan, Meghan Cansler, Megan Null, Wilfried Bardet, Kenneth W Jackson, Deborah A Lewinsohn, William Hildebrand, David M Lewinsohn
HLA-E is a non-conventional MHC Class I molecule that has been recently demonstrated to present pathogen-derived ligands, resulting in the TCR-dependent activation of αβ CD8+ T cells. The goal of this study was to characterize the ligandome displayed by HLA-E following infection with Mycobacterium tuberculosis (Mtb) using an in-depth mass spectrometry approach. Here we identified 28 Mtb ligands derived from 13 different source proteins, including the Esx family of proteins. When tested for activity with CD8+ T cells isolated from sixteen donors, nine of the ligands elicited an IFN-γ response from at least one donor, with fourteen of 16 donors responding to the Rv0634A19-29 peptide...
2017: PloS One
https://www.readbyqxmd.com/read/29174699/neoteric-advancement-in-tb-drugs-and-an-overview-on-the-anti-tubercular-role-of-peptides-through-computational-approaches
#8
REVIEW
Ameer Khusro, Chirom Aarti, Alberto Barbabosa-Pliego, Abdelfattah Z M Salem
Tuberculosis (TB) is a devastating threat to human health whose treatment without the emergence of drug resistant Mycobacterium tuberculosis (M. tuberculosis) is the million-dollar question at present. The pathogenesis of M. tuberculosis has been extensively studied which represents unique defence strategies by infecting macrophages. Several anti-tubercular drugs with varied mode of action and administration from diversified sources have been used for the treatment of TB that later contributed to the emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB)...
November 23, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29171448/vitamin-d-cell-death-pathways-and-tuberculosis
#9
Manik Retno Wahyunitisari, Ni Made Mertaniasih, Muhammad Amin, Wayan T Artama, Eko B Koendhori
BACKGROUND: Mycobacterium tuberculosis induces cellular necrosis that could promote spread of infection. The aim of this study is to analyze the effects of Vitamin D3 supplementation to improve the effectiveness of 2nd-line anti-tuberculosis (TB) drug therapy, especially in relation with cell death pathways. METHODS: Mus musculus C3HeB/FeJ was randomly divided into four groups containing eight animals each. The 1st group (G1), consisting of mice that were intratracheally infected with multidrug-resistant strain of M...
October 2017: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/29163906/biosynthesis-of-methyl-proline-containing-griselimycins-natural-products-with-anti-tuberculosis-activity
#10
Peer Lukat, Yohei Katsuyama, Silke Wenzel, Tina Binz, Claudia König, Wulf Blankenfeldt, Mark Brönstrup, Rolf Müller
Griselimycins (GMs) are depsidecapeptides with superb anti-tuberculosis activity. They contain up to three (2S,4R)-4-methyl-prolines (4-MePro), of which one blocks oxidative degradation and increases metabolic stability in animal models. The natural congener with this substitution is only a minor component in fermentation cultures. We showed that this product can be significantly increased by feeding the reaction with 4-MePro and we investigated the molecular basis of 4-MePro biosynthesis and incorporation...
November 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/29163551/host-antimicrobial-peptides-the-promise-of-new-treatment-strategies-against-tuberculosis
#11
REVIEW
Javier Arranz-Trullén, Lu Lu, David Pulido, Sanjib Bhakta, Ester Boix
Tuberculosis (TB) continues to be a devastating infectious disease and remerges as a global health emergency due to an alarming rise of antimicrobial resistance to its treatment. Despite of the serious effort that has been applied to develop effective antitubercular chemotherapies, the potential of antimicrobial peptides (AMPs) remains underexploited. A large amount of literature is now accessible on the AMP mechanisms of action against a diversity of pathogens; nevertheless, research on their activity on mycobacteria is still scarce...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29143375/an-immunoinformatics-approach-to-define-t-cell-epitopes-from-polyketide-and-non-ribosomal-peptide-synthesis-proteins-of-mycobacterium-tuberculosis-as-potential-vaccine-candidates
#12
S Dhivya, V Baskar, S R Kumar, R Sathishkumar
The role of polyketide and non-ribosomal proteins from the class of small molecule metabolism of Mycobacterium tuberculosis is well documented in envelope organization, virulence, and pathogenesis. Consequently, the identification of T cell epitopes from these proteins could serve to define potential antigens for the development of vaccines. Fourty-one proteins from polyketide and non-ribosomal peptide synthesis of small molecule metabolism proteins of M tuberculosis H37Rv were analyzed computationally for the presence of HLA class I binding nanomeric peptides...
November 16, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29135655/neutrophil-activation-and-enhanced-release-of-granule-products-in-hiv-tb-immune-reconstitution-inflammatory-syndrome
#13
Justine K Nakiwala, Naomi F Walker, Collin R Diedrich, William Worodria, Graeme Meintjes, Robert J Wilkinson, Harriet Mayanja-Kizza, Robert Colebunders, Luc Kestens, Katalin A Wilkinson, David M Lowe
BACKGROUND: Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) remains incompletely understood. Neutrophils are implicated in tuberculosis pathology but detailed investigations in TB-IRIS are lacking. We sought to further explore the biology of TB-IRIS and in particular the role of neutrophils. SETTING: Two observational, prospective cohort studies in HIV/TB co-infected patients starting antiretroviral therapy, one to analyze gene expression and subsequently one to explore neutrophil biology...
November 6, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29125580/structural-diversity-biological-properties-and-applications-of-natural-products-from-cyanobacteria-a-review
#14
REVIEW
Sayed Asmat Ali Shah, Najeeb Akhter, Bibi Nazia Auckloo, Ishrat Khan, Yanbin Lu, Kuiwu Wang, Bin Wu, Yue-Wei Guo
Nowadays, various drugs on the market are becoming more and more resistant to numerous diseases, thus declining their efficacy for treatment purposes in human beings. Antibiotic resistance is one among the top listed threat around the world which eventually urged the discovery of new potent drugs followed by an increase in the number of deaths caused by cancer due to chemotherapy resistance as well. Accordingly, marine cyanobacteria, being the oldest prokaryotic microorganisms belonging to a monophyletic group, have proven themselves as being able to generate pharmaceutically important natural products...
November 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29124751/detailed-characterization-of-human-mycobacterium-tuberculosis-specific-hla-e-restricted-cd8-t-cells
#15
Teresa Prezzemolo, Krista E van Meijgaarden, Kees Lmc Franken, Nadia Caccamo, Francesco Dieli, Tom Hm Ottenhoff, Simone A Joosten
HLA-E presented antigens are interesting targets for vaccination given HLA-Es' essentially monomorphic nature. We have shown previously that Mycobacterium tuberculosis (Mtb) peptides are presented by HLA-E to CD8(+) effector T-cells, but the precise phenotype and functional capacity of these cells remains poorly characterized. We have developed and utilized in this study a new protocol combining HLA-E tetramer with intracellular staining for cytokines, transcription factors and cytotoxic molecules to characterize these cells in depth...
November 9, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29123441/evaluation-of-the-bactec-mgit-960-sl-dst-kit-and-the-genotype-mtbdrsl-test-for-detecting-extensively-drug-resistant-tuberculosis-cases
#16
Kemal Tekin, Ali Albay, Hulya Simsek, Ali Korhan Sig, Mustafa Guney
Objective: The present study aimed to evaluate the performances of the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test for detecting second-line antituberculosis drug resistance in Multidrug-resistant TB (MDR-TB) cases. Materials and Methods: Forty-six MDR-TB strains were studied. Second-line antituberculosis drug resistances were detected using the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test. The Middlebrook 7H10 agar proportion method was used as the reference test...
October 2017: Eurasian Journal of Medicine
https://www.readbyqxmd.com/read/29100330/protective-cellular-immunity-generated-by-cross-presenting-recombinant-overlapping-peptide-proteins
#17
Lili Cai, Jianbo Zhang, Renying Zhu, Weixing Shi, Xiaobing Xia, Mark Edwards, William Finch, Anthony Coombs, Ju Gao, Kangwen Chen, Sophie Owen, Shisong Jiang, Wenshu Lu
Priming of naive CD8(+) and CD4(+) T cells by dendritic cells (DCs) requires effective antigen presentation on both MHC class I and II molecules. We have developed a novel technology to use recombinant overlapping peptides (ROP) that stimulate both CD8(+) and CD4(+) T cell immune responses. The single chain protein of a ROP is made up of overlapping peptides linked by the target sequence (LRMK) for cathepsin S, a protease found in the endosomes of DCs. We designed synthetic genes encoding ROPs derived from ovalbumin (OVA), tuberculosis protein (CFP10-ESAT6), human papilloma virus (HPV) protein (E7) and survivin, a protein commonly over-expressed in tumour cells...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29089034/rapid-diagnosis-of-new-and-relapse-tuberculosis-by-quantification-of-a-circulating-antigen-in-hiv-infected-adults-in-the-greater-houston-metropolitan-area
#18
Jia Fan, Hedong Zhang, Duc T Nguyen, Christopher J Lyon, Charles D Mitchell, Zhen Zhao, Edward A Graviss, Ye Hu
BACKGROUND: HIV-associated immune defects inhibit tuberculosis (TB) diagnosis, promote development of extrapulmonary TB and paucibacillary pulmonary TB cases with atypical radiographic features, and increase TB relapse rates. We therefore assessed the diagnostic performance of a novel assay that directly quantitates serum levels of the Mycobacterium tuberculosis (Mtb) virulence factor 10-kDa culture filtrate protein (CFP-10) to overcome limitations associated with detecting Mtb bacilli in sputum or tissue biopsies...
November 1, 2017: BMC Medicine
https://www.readbyqxmd.com/read/29079429/antimicrobial-peptides-as-an-alternative-to-anti-tuberculosis-drugs
#19
REVIEW
Manaf AlMatar, Essam A Makky, Gülfer Yakıcı, Işıl Var, Begüm Kayar, Fatih Köksal
Tuberculosis (TB) presently accounts for high global mortality and morbidity rates, despite the introduction four decades ago of the affordable and efficient four-drugs (isoniazid, rifampicin, pyrazinamide and ethambutol). Thus, a strong need exists for new drugs with special structures and uncommon modes of action to effectively overcome M. tuberculosis. Within this scope, antimicrobial peptides (AMPs), which are small, cationic and amphipathic peptides that comprise a section of the innate immune system, are currently the leading potential agents for the treatment of TB...
October 24, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29050772/ctl-immunogenicity-of-rv3615c-antigen-and-diagnostic-performances-of-an-esat-6-cfp-10-rv3615c-antigen-cocktail-for-mycobacterium-tuberculosis-infection
#20
Shuguang Tan, Nan Lin, Mingxiang Huang, Qing Wang, Yunhong Tan, Bingxi Li, Ning Zhang, Tianling Guo, Yingbin Cui, Xinchao Chen, Dongping Wang, Jue Wang, Haixia Xiao, William J Liu, Jinghua Yan, Catherine W-H Zhang, Cui Hua Liu, Kanglin Wan, George F Gao
T cell immune responses have played pivotal roles in host immune protection against Mycobacterium tuberculosis (MTB) infection. MTB specific antigen, Rv3615c (EspC), was identified to be as immunodominant as the well-known ESAT-6 and CFP-10, and has brought promising expectations to more sensitive T-cell based diagnosis and vaccine development. However, limited knowledge about the immunogenicity and diagnostic values of this antigen has restricted its application in clinical practice. Herein, the Rv3615c antigen was identified as a robust CTL immunoantigen with broadly cross-human leucocyte antigen (HLA) allele recognized peptides which may contribute to the broad recognition of Rv3615c antigen among the population...
December 2017: Tuberculosis
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