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peptides and tuberculosis

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https://www.readbyqxmd.com/read/29143375/an-immunoinformatics-approach-to-define-t-cell-epitopes-from-polyketide-and-non-ribosomal-peptide-synthesis-proteins-of-mycobacterium-tuberculosis-as-potential-vaccine-candidates
#1
S Dhivya, V Baskar, S R Kumar, R Sathishkumar
The role of polyketide and non-ribosomal proteins from the class of small molecule metabolism of Mycobacterium tuberculosis is well documented in envelope organization, virulence, and pathogenesis. Consequently, the identification of T cell epitopes from these proteins could serve to define potential antigens for the development of vaccines. Fourty-one proteins from polyketide and non-ribosomal peptide synthesis of small molecule metabolism proteins of M tuberculosis H37Rv were analyzed computationally for the presence of HLA class I binding nanomeric peptides...
November 16, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29135655/neutrophil-activation-and-enhanced-release-of-granule-products-in-hiv-tb-immune-reconstitution-inflammatory-syndrome
#2
Justine K Nakiwala, Naomi F Walker, Collin R Diedrich, William Worodria, Graeme Meintjes, Robert J Wilkinson, Harriet Mayanja-Kizza, Robert Colebunders, Luc Kestens, Katalin A Wilkinson, David M Lowe
BACKGROUND: Tuberculosis Immune Reconstitution Inflammatory Syndrome (TB-IRIS) remains incompletely understood. Neutrophils are implicated in tuberculosis pathology but detailed investigations in TB-IRIS are lacking. We sought to further explore the biology of TB-IRIS and in particular the role of neutrophils. SETTING: Two observational, prospective cohort studies in HIV/TB co-infected patients starting antiretroviral therapy, one to analyze gene expression and subsequently one to explore neutrophil biology...
November 6, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29125580/structural-diversity-biological-properties-and-applications-of-natural-products-from-cyanobacteria-a-review
#3
REVIEW
Sayed Asmat Ali Shah, Najeeb Akhter, Bibi Nazia Auckloo, Ishrat Khan, Yanbin Lu, Kuiwu Wang, Bin Wu, Yue-Wei Guo
Nowadays, various drugs on the market are becoming more and more resistant to numerous diseases, thus declining their efficacy for treatment purposes in human beings. Antibiotic resistance is one among the top listed threat around the world which eventually urged the discovery of new potent drugs followed by an increase in the number of deaths caused by cancer due to chemotherapy resistance as well. Accordingly, marine cyanobacteria, being the oldest prokaryotic microorganisms belonging to a monophyletic group, have proven themselves as being able to generate pharmaceutically important natural products...
November 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29124751/detailed-characterization-of-human-mycobacterium-tuberculosis-specific-hla-e-restricted-cd8-t-cells
#4
Teresa Prezzemolo, Krista E van Meijgaarden, Kees Lmc Franken, Nadia Caccamo, Francesco Dieli, Tom Hm Ottenhoff, Simone A Joosten
HLA-E presented antigens are interesting targets for vaccination given HLA-Es' essentially monomorphic nature. We have shown previously that Mycobacterium tuberculosis (Mtb) peptides are presented by HLA-E to CD8(+) effector T-cells, but the precise phenotype and functional capacity of these cells remains poorly characterized. We have developed and utilized in this study a new protocol combining HLA-E tetramer with intracellular staining for cytokines, transcription factors and cytotoxic molecules to characterize these cells in depth...
November 9, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/29123441/evaluation-of-the-bactec-mgit-960-sl-dst-kit-and-the-genotype-mtbdrsl-test-for-detecting-extensively-drug-resistant-tuberculosis-cases
#5
Kemal Tekin, Ali Albay, Hulya Simsek, Ali Korhan Sig, Mustafa Guney
Objective: The present study aimed to evaluate the performances of the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test for detecting second-line antituberculosis drug resistance in Multidrug-resistant TB (MDR-TB) cases. Materials and Methods: Forty-six MDR-TB strains were studied. Second-line antituberculosis drug resistances were detected using the BACTEC MGIT 960 SL DST kit and the GenoType MTBDRsl test. The Middlebrook 7H10 agar proportion method was used as the reference test...
October 2017: Eurasian Journal of Medicine
https://www.readbyqxmd.com/read/29100330/protective-cellular-immunity-generated-by-cross-presenting-recombinant-overlapping-peptide-proteins
#6
Lili Cai, Jianbo Zhang, Renying Zhu, Weixing Shi, Xiaobing Xia, Mark Edwards, William Finch, Anthony Coombs, Ju Gao, Kangwen Chen, Sophie Owen, Shisong Jiang, Wenshu Lu
Priming of naive CD8(+) and CD4(+) T cells by dendritic cells (DCs) requires effective antigen presentation on both MHC class I and II molecules. We have developed a novel technology to use recombinant overlapping peptides (ROP) that stimulate both CD8(+) and CD4(+) T cell immune responses. The single chain protein of a ROP is made up of overlapping peptides linked by the target sequence (LRMK) for cathepsin S, a protease found in the endosomes of DCs. We designed synthetic genes encoding ROPs derived from ovalbumin (OVA), tuberculosis protein (CFP10-ESAT6), human papilloma virus (HPV) protein (E7) and survivin, a protein commonly over-expressed in tumour cells...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29089034/rapid-diagnosis-of-new-and-relapse-tuberculosis-by-quantification-of-a-circulating-antigen-in-hiv-infected-adults-in-the-greater-houston-metropolitan-area
#7
Jia Fan, Hedong Zhang, Duc T Nguyen, Christopher J Lyon, Charles D Mitchell, Zhen Zhao, Edward A Graviss, Ye Hu
BACKGROUND: HIV-associated immune defects inhibit tuberculosis (TB) diagnosis, promote development of extrapulmonary TB and paucibacillary pulmonary TB cases with atypical radiographic features, and increase TB relapse rates. We therefore assessed the diagnostic performance of a novel assay that directly quantitates serum levels of the Mycobacterium tuberculosis (Mtb) virulence factor 10-kDa culture filtrate protein (CFP-10) to overcome limitations associated with detecting Mtb bacilli in sputum or tissue biopsies...
November 1, 2017: BMC Medicine
https://www.readbyqxmd.com/read/29079429/antimicrobial-peptides-as-an-alternative-to-anti-tuberculosis-drugs
#8
REVIEW
Manaf AlMatar, Essam A Makky, Gülfer Yakıcı, Işıl Var, Begüm Kayar, Fatih Köksal
Tuberculosis (TB) presently accounts for high global mortality and morbidity rates, despite the introduction four decades ago of the affordable and efficient four-drugs (isoniazid, rifampicin, pyrazinamide and ethambutol). Thus, a strong need exists for new drugs with special structures and uncommon modes of action to effectively overcome M. tuberculosis. Within this scope, antimicrobial peptides (AMPs), which are small, cationic and amphipathic peptides that comprise a section of the innate immune system, are currently the leading potential agents for the treatment of TB...
October 24, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29050772/ctl-immunogenicity-of-rv3615c-antigen-and-diagnostic-performances-of-an-esat-6-cfp-10-rv3615c-antigen-cocktail-for-mycobacterium-tuberculosis-infection
#9
Shuguang Tan, Nan Lin, Mingxiang Huang, Qing Wang, Yunhong Tan, Bingxi Li, Ning Zhang, Tianling Guo, Yingbin Cui, Xinchao Chen, Dongping Wang, Jue Wang, Haixia Xiao, William J Liu, Jinghua Yan, Catherine W-H Zhang, Cui Hua Liu, Kanglin Wan, George F Gao
T cell immune responses have played pivotal roles in host immune protection against Mycobacterium tuberculosis (MTB) infection. MTB specific antigen, Rv3615c (EspC), was identified to be as immunodominant as the well-known ESAT-6 and CFP-10, and has brought promising expectations to more sensitive T-cell based diagnosis and vaccine development. However, limited knowledge about the immunogenicity and diagnostic values of this antigen has restricted its application in clinical practice. Herein, the Rv3615c antigen was identified as a robust CTL immunoantigen with broadly cross-human leucocyte antigen (HLA) allele recognized peptides which may contribute to the broad recognition of Rv3615c antigen among the population...
December 2017: Tuberculosis
https://www.readbyqxmd.com/read/29038252/a-bacteriophage-capsid-protein-is-an-inhibitor-of-a-conserved-transcription-terminator-of-various-bacterial-pathogens
#10
Gairika Ghosh, Jayavardhana Reddy, Susmit Sambhare, Ranjan Sen
Rho is a hexameric molecular motor that functions as a conserved transcription terminator in majority of the bacterial species, which is a potential drug target. Psu is a bacteriophage P4 capsid protein that inhibits E.coli Rho by obstructing its ATPase and translocase activities. Here, we explored the anti-Rho activity of Psu for the Rho proteins from different pathogens. Sequence alignment and homology modelling of Rho proteins from pathogenic bacteria revealed the conserved nature of the Psu-interacting regions in all these proteins...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29035048/structural-sequencing-of-oligopeptides-aided-by-1-h-iterative-full-spin-analysis
#11
Wei Gao, James B McAlpine, Mary P Choules, José G Napolitano, David C Lankin, Charlotte Simmler, Ngoc Anh Ho, Hanki Lee, Joo-Won Suh, Ian W Burton, Sanghyun Cho, Scott G Franzblau, Shao-Nong Chen, Guido F Pauli
This report describes an approach using (1)H NMR iterative full-spin analysis (HiFSA) to extract definitive structural information on unknown peptides from 1D (1)H NMR data. By comparing the experimental data and HiFSA fingerprint of a known analogue, it is possible to isolate the characteristic (1)H subspectrum of the different amino acids and, thus, elucidate the structure of the peptide. To illustrate this methodology, a comprehensive analysis of five new anti-Mycobacterium tuberculosis peptides (2-6), all analogues of ecumicin (1), was carried out...
October 27, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/29034633/-comparison-of-effects-of-staphylococcal-nuclease-a-fused-with-different-exogenous-dna-fragments
#12
Lixia Fu, Dejun Ji, Xubin Lu, Xian'gan Han, Wenzhi Wei
Staphylococcal nuclease A (SNA) may be used to produce bacterial ghosts for further inactivation of host bacteria and elimination of residual genetic materials. It is still controversial if SNA without signal peptide can be secreted to extracellular matrix and if fusion with other peptide is required for its function in the cytoplasm of host bacteria. To clarify this dispute, a series of temperature-inducible plasmids carrying SNA alone or SNA fused with partial sequences of λ phage cro gene (cSNA) or Mycobacterium tuberculosis urease gene (uSNA) were constructed and evaluated in Escherichia coli...
December 25, 2016: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/28994205/proteomics-and-network-analyses-reveal-inhibition-of-akt-mtor-signaling-in-cd4-t-cells-by-mycobacterium-tuberculosis-mannose-capped-lipoarabinomannan
#13
Ahmad F Karim, Obondo J Sande, Sara E Tomeckho, Xuedong Ding, Ming Li, Sean Maxwell, Rob M Ewing, Clifford V Harding, Roxana E Rojas, Mark R Chance, W Henry Boom
Mycobacterium tuberculosis (Mtb) cell wall glycolipid Mannose-capped Lipoarabinomannan (ManLAM) inhibits CD4(+) T cell activation by inhibiting proximal T cell receptor (TCR) signaling when activated by anti-CD3. To understand the impact of ManLAM on CD4(+) T cell function when both the TCR-CD3 complex and major co-stimulator CD28 are engaged, we performed label-free quantitative mass spectrometry (MS) and network analysis. Mixed effect model analysis of peptide intensity identified 149 unique peptides representing 131 proteins that were differentially regulated by ManLAM in anti-CD3 and anti-CD28 activated CD4(+) T cells...
October 10, 2017: Proteomics
https://www.readbyqxmd.com/read/28993827/synthesis-and-biological-evaluation-of-novel-teixobactin-analogues
#14
Christian E Schumacher, Paul W R Harris, Xiao-Bo Ding, Bernard Krause, Tom H Wright, Gregory M Cook, Daniel P Furkert, Margaret A Brimble
The cyclic depsipeptide, teixobactin, possesses promising activity against a range of antimicrobial-resistant (AMR) pathogenic bacteria, including Staphylococcus aureus and Mycobacterium tuberculosis. Teixobactin contains a number of non-canonical residues, including the synthetically challenging amino acid, l-allo-enduracididine, complicating clinical application of this peptide. Herein, we report the synthesis of six analogues of teixobactin, in which the non-canonical l-allo-enduracididine amino acid is replaced by isosteric, commercially available Fmoc-amino acid building blocks...
October 25, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28985739/a-lipidated-peptide-of-mycobacterium-tuberculosis-resuscitates-the-protective-efficacy-of-bcg-vaccine-by-evoking-memory-t-cell-immunity
#15
Pradeep K Rai, Sathi Babu Chodisetti, Weiguang Zeng, Sajid Nadeem, Sudeep K Maurya, Susanta Pahari, Ashok K Janmeja, David C Jackson, Javed N Agrewala
BACKGROUND: The current BCG vaccine induces only short-term protection against Mycobacterium tuberculosis (Mtb), suggesting its failure to generate long-lasting memory T cells. Previously, we have demonstrated that a self-adjuvanting peptide of Mtb (L91), successfully generated enduring memory Th1 cells. Consequently, we investigated if L91 was able to recuperate BCG potency in perpetuating the generation of memory T cells and protection against Mtb infected mice. METHODS: In the present study, we evaluated the potency of a self adjuvanting Mtb peptide vaccine L91 in invigorating BCG immune response against Mtb in mice...
October 6, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28982200/a-baculovirus-conjugated-mimotope-vaccine-targeting-mycobacterium-tuberculosis-lipoarabinomannan
#16
Hyun-Jin Shin, Luis H Franco, Vidhya R Nair, Angela C Collins, Michael U Shiloh
Mycobacterium tuberculosis, the causative agent of tuberculosis, is a major cause of morbidity and mortality worldwide. However, an effective vaccine for M. tuberculosis is lacking. We panned a phage display library using monoclonal antibodies against M. tuberculosis liporabinomannan (LAM), an important component of the M. tuberculosis cell wall, and identified two peptide sequences, HSFKWLDSPRLR or SGVYKVAYDWQH, with high antibody affinity after multiple rounds of panning. Only the HSFKWLDSPRLR peptide induced an anti-LAM response when conjugated to either keyhole limpet hemocyanin (KLH) or to the baculovirus Autographa californica multicapsid nucleopolyherovirus (AcMNPV) when introduced into mice by injection or via intranasal inoculation, respectively...
2017: PloS One
https://www.readbyqxmd.com/read/28978467/m-%C3%A2-tuberculosis-initiated-human-mannose-receptor-signaling-regulates-macrophage-recognition-and-vesicle-trafficking-by-fcr%C3%AE-chain-grb2-and-shp-1
#17
Murugesan V S Rajaram, Eusondia Arnett, Abul K Azad, Evelyn Guirado, Bin Ni, Abigail D Gerberick, Li-Zhen He, Tibor Keler, Lawrence J Thomas, William P Lafuse, Larry S Schlesinger
Despite its prominent role as a C-type lectin (CTL) pattern recognition receptor, mannose receptor (MR, CD206)-specific signaling molecules and pathways are unknown. The MR is highly expressed on human macrophages, regulating endocytosis, phagocytosis, and immune responses and mediating Mycobacterium tuberculosis (M.tb) phagocytosis by human macrophages, thereby limiting phagosome-lysosome (P-L) fusion. We identified human MR-associated proteins using phosphorylated and non-phosphorylated MR cytoplasmic tail peptides...
October 3, 2017: Cell Reports
https://www.readbyqxmd.com/read/28972145/structural-analyses-of-the-mazef4-toxin-antitoxin-pair-in-mycobacterium-tuberculosis-provide-evidence-for-a-unique-extracellular-death-factor
#18
Do-Hwan Ahn, Ki-Young Lee, Sang Jae Lee, Sung Jean Park, Hye-Jin Yoon, Soon-Jong Kim, Bong-Jin Lee
The bacterial toxin-antitoxin MazEF system in the tuberculosis (TB)-causing bacterium Mycobacterium tuberculosis is activated under unfavorable conditions, including starvation, antibiotic exposure, and oxidative stress. This system contains the ribonucleolytic enzyme MazF and has emerged as a promising drug target for TB treatments targeting the latent stage of M. tuberculosis infection and reportedly mediates a cell death process via a peptide called extracellular death factor (EDF). Although it is well established that the increase in EDFmediated toxicity of MazF drives a cell-killing phenomenon, the molecular details are poorly understood...
October 2, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28956840/investigation-of-the-n-terminus-amino-function-of-arg10-teixobactin
#19
Shimaa A H Abdel Monaim, Sikabwe Noki, Estelle J Ramchuran, Ayman El-Faham, Fernando Albericio, Beatriz G de la Torre
Teixobactin is a recently described antimicrobial peptide that shows high activity against gram-positive bacteria as well as mycobacterium tuberculosis. Due to both its structure as a head-to-side chain cyclodepsipeptide and its activity, it has attracted the attention of several research groups. In this regard, a large number of analogs with substitutions in both the cycle and the tail has been described. Here, we report the contribution of the N-terminus residue, N-Me-d-Phe, to the activity of Arg10-teixobactin...
September 28, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28956425/serum-level-of-cathelicidin-ll-37-in-patients-with-active-tuberculosis-and-other-infectious-diseases
#20
K Majewski, J Agier, E Kozłowska, E Brzezińska-Błaszczyk
A growing body of evidence indicates the role of cathelicidin LL-37, a member of the antimicrobial peptide family, in host innate defense mechanisms. The important role of this peptide in infectious diseases is also suggested, however, to date, data relating to LL-37 expression in the course of bacterial infections are far from complete. Therefore, the aim of the present study was to determine LL-37 serum levels in adult patients with pulmonary tuberculosis (TB). For comparison, circulating LL-37 levels in patients with pneumonia induced by Gram-positive or Gram-negative bacteria species and in healthy subjects were evaluated...
July 2017: Journal of Biological Regulators and Homeostatic Agents
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