Kathleen E DelGiorno, Chi-Yeh Chung, Vera Vavinskaya, H Carlo Maurer, Sammy Weiser Novak, Nikki K Lytle, Zhibo Ma, Rajshekhar R Giraddi, Dezhen Wang, Linjing Fang, Razia F Naeem, Leonardo R Andrade, Wahida H Ali, Hubert Tseng, Crystal Tsui, Vikas B Gubbala, Maya Ridinger-Saison, Makoto Ohmoto, Galina A Erikson, Carolyn O'Connor, Maxim Nikolaievich Shokhirev, Nasun Hah, Yoshihiro Urade, Ichiro Matsumoto, Susan M Kaech, Pankaj K Singh, Uri Manor, Kenneth P Olive, Geoffrey M Wahl
BACKGROUND & AIMS: Development of pancreatic ductal adenocarcinoma (PDA) involves acinar to ductal metaplasia and genesis of tuft cells. It has been a challenge to study these rare cells because of the lack of animal models. We investigated the role of tuft cells in pancreatic tumorigenesis. METHODS: We performed studies with LSL-KrasG12D/+ ;Ptf1aCre/+ mice (KC; develop pancreatic tumors), KC mice crossed with mice with pancreatic disruption of Pou2f3 (KPouC mice; do not develop tuft cells), or mice with pancreatic disruption of the hematopoietic prostaglandin D synthase gene (Hpgds, KHC mice) and wild-type mice...
November 2020: Gastroenterology