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https://www.readbyqxmd.com/read/28214241/improvement-of-operational-stability-of-ogataea-minuta-carbonyl-reductase-for-chiral-alcohol-production
#1
Kohsuke Honda, Mizuha Inoue, Tomohiro Ono, Kenji Okano, Yasumasa Dekishima, Hiroshi Kawabata
Directed evolution of enantio-selective carbonyl reductase from Ogataea minuta was conducted to improve the operational stability of the enzyme. A mutant library was constructed by an error-prone PCR and screened using a newly developed colorimetric assay. The stability of a mutant with two amino acid substitutions was significantly higher than that of the wild type at 50°C in the presence of dimethyl sulfoxide. Site-directed mutagenesis analysis showed that the improved stability of the enzyme can be attributed to the amino acid substitution of V166A...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28210755/assessment-of-quinoxyfen-phototransformation-pathways-by-liquid-chromatography-coupled-to-accurate-mass-spectrometry
#2
Priscila Ferri, María Ramil, Isaac Rodríguez, Rosângela Bergamasco, Angélica Marquetotti Salcedo Vieira, Rafael Cela
Quinoxyfen has been recently identified as a priority hazardous substance in the field of the European water policy. In this work, its fate in aqueous samples and solid supports under UV and solar radiation is investigated. Diverse degradation experiments were carried out, at lab scale, using spiked aliquots of different aqueous matrices (ultrapure, treated wastewater and river water) irradiated at different wavelengths (λ = 254 nm, λ = 365 nm and solar light). Half-lives of quinoxyfen (2-26 min) depended on the wavelength and the intensity of radiation whilst the nature of the aqueous matrix did not play an important role in degradation kinetics...
February 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28208197/reversal-of-direct-oral-anticoagulants-current-status-and-future-directions
#3
Jeffrey I Weitz
Direct oral anticoagulants (DOACs) are increasingly used for prevention and treatment of venous thromboembolism and for prevention of stroke in patients with nonvalvular atrial fibrillation. In phase III clinical trials that included more than 100,000 patients, the DOACs were at least as effective as vitamin K antagonists (VKAs) and were associated with less serious bleeding, particularly less intracranial bleeding. Real-world evidence supports these outcomes. Despite this, some physicians and patients are concerned about serious bleeding or emergencies unless specific reversal agents for the DOACs are available...
February 2017: Seminars in Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28203639/determination-of-kinetic-bioconcentration-in-mussels-after-short-term-exposure-to-polycyclic-aromatic-hydrocarbons
#4
Ledicia Rey-Salgueiro, Elena Martínez-Carballo, Antonio Cid, Jesús Simal-Gándara
The kinetic bioconcentration of N-heterocyclic aromatic hydrocarbons and polycyclic aromatic hydrocarbons in mussels (Mytilus galloprovincialis) after short waterborne exposure was studied. Benzo[a]pyrene (BaP), its analogue azaarene 10-azabenzo[a]pyrene (AzaBaP), and their mixture (Mix), were selected to monitor the changes in water concentrations over three days. Decay of both PAHs concentrations in water after 24 h of waterborne exposure to mussels at levels of 10 and 100 μg/L follows a first order kinetic with half-lives of 4-5 h, with residual levels of PAHs below 7%...
January 2017: Heliyon
https://www.readbyqxmd.com/read/28193575/applying-sewage-epidemiology-approach-to-estimate-illicit-drug-consumption-in-a-tropical-context-bias-related-to-sewage-temperature-and-ph
#5
Damien A Devault, Yves Lévi, Sara Karolak
Illicit drug consumption can be estimated from drug target residue (DTR) in wastewater, with the reliability of results being partly linked to DTR stability in the sewage network. However, wastewater temperature and pH drive the stability of molecules and, in this context, tropical conditions must be studied to specify the impact of residence time in the sewage network on DTR degradation. Warmth enhances biotic and abiotic processes such as degradation, leading to a decrease in oxygen content, and consequently, early diagenesis conditions in wastewater...
February 10, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28188804/metabolite-profiling-of-infection-associated-metabolic-markers-of-onchocerciasis
#6
Sasisekhar Bennuru, Sara Lustigman, David Abraham, Thomas B Nutman
The global efforts for onchocerciasis elimination may require additional tools (safe micro and macrofilaricidal drugs, vaccines and biomarkers) as elimination efforts move toward the "end game". Efforts toward the identification of suitable biomarkers have focused on specific protein(s) and/or nucleic acids, but metabolites present an alternative option as they have limited half-lives and are the result of combinatorial effects. In comparison to previously used methodology of LC-MS for metabolomic approaches, we used a non-targeted capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) to analyze the serum metabolic profiles of Ov-infected and -uninfected individuals (n=20)...
February 7, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/28188285/rna-binding-protein-quaking-stabilizes-sirt2-mrna-during-oligodendroglial-differentiation
#7
Merlin P Thangaraj, Kendra L Furber, Jotham K Gan, Shaoping Ji, Larhonda Sobchishin, J Ronald Doucette, Adil J Nazarali
Myelination is controlled by timely expression of genes involved in the differentiation of oligodendrocyte precursor cells (OPCs) into myelinating oligodendrocytes (OLs). Sirtuin 2 (SIRT2), a NAD+-dependent deacetylase, plays a critical role in OL differentiation by promoting both arborization and downstream expression of myelin specific genes. However, the mechanisms involved in regulating SIRT2 expression during OL development are largely unknown. The RNA binding protein Quaking (QKI) plays an important role in myelination by post-transcriptionally regulating the expression of several myelin specific genes...
February 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28184964/absorption-metabolism-and-excretion-of-14-c-ponatinib-after-a-single-oral-dose-in-humans
#8
Yihua E Ye, Caroline N Woodward, Narayana I Narasimhan
PURPOSE: Ponatinib is a novel tyrosine kinase inhibitor (TKI) specifically designed to inhibit native and mutated BCR-ABL. In the United States, ponatinib has received accelerated approval for adults with T315I-positive chronic myeloid leukemia (CML) or T315I (gatekeeper mutation)-positive, Philadelphia chromosome-positive, acute lymphoblastic leukemia (Ph + ALL), and patients with CML or Ph + ALL for whom no other TKI therapy is indicated. The objective of this phase 1, mass balance study was to evaluate the absorption, metabolism, and excretion of [(14)C]ponatinib in healthy subjects...
February 9, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28183350/pharmacokinetic-and-pharmacodynamic-modeling-of-sarafloxacin-against-avian-pathogenic-escherichia-coli-in-muscovy-ducks
#9
Yang Yu, Yu Feng Zhou, Jian Sun, Wei Shi, Xiao Ping Liao, Ya Hong Liu
BACKGROUND: This study focused on utilizing pharmacokinetics/pharmacodynamics (PK/PD) modeling to optimize therapeutic dosage regimens of sarafloxacin against avian pathogenic Escherichia. coli O78 strain in Muscovy ducks. The ex vivo PK/PD study of sarafloxacin was conducted in Muscovy ducks after intravenous (i.v.) and oral (p.o.) administrations at a single dose of 10 mg/kg bodyweight (BW). The serum samples were analyzed by reverse phase high-performance liquid chromatography (RP-HPLC) using a fluorescence detection method...
February 10, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28177830/analysis-of-codon-usage-patterns-in-morus-notabilis-based-on-genome-and-transcriptome-data
#10
Yan Wen, Ziliang Zou, Hongshun Li, Zhonghuai Xiang, Ningjia He
Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tree), and analyze genome-wide codon usage in 12 other Rosales species. The codon usage of M. notabilis was affected by nucleotide composition, mutation pressure, nature selection, and gene expression level...
January 3, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177236/degradation-dynamics-and-dietary-risk-assessments-of-two-neonicotinoid-insecticides-during-lonicera-japonica-planting-drying-and-tea-brewing-processes
#11
Qingkui Fang, Yanhong Shi, Haiqun Cao, Zhou Tong, Jinjing Xiao, Min Liao, Xiangwei Wu, Rimao Hua
The degradation dynamics and dietary risk assessments of thiamethoxam and thiacloprid during Lonicera japonica planting, drying and tea brewing processes were systematically investigated using high-performance liquid chromatography. The half-lives of thiamethoxam and thiacloprid were 1.0-4.1 d in the honeysuckle flowers and leaves, degradation rate constants k ranged from -0.169 to -0.696. And the safety interval time was 7 d. The sun- and oven-drying (70 °C) percent digestions were 59.4-81.0% for the residues, which were higher than the shade- and oven-drying percent at lower temperatures (30, 40, 50 and 60 °C, which ranged from 37...
February 8, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28163713/homologous-u-box-e3-ubiquitin-ligases-ospub2-and-ospub3-are-involved-in-the-positive-regulation-of-low-temperature-stress-response-in-rice-oryza-sativa-l
#12
Mi Young Byun, Li Hua Cui, Tae Kyung Oh, Ye-Jin Jung, Andosung Lee, Ki Youl Park, Bin Goo Kang, Woo Taek Kim
Rice U-box E3 Ub ligases (OsPUBs) are implicated in biotic stress responses. However, their cellular roles in response to abiotic stress are poorly understood. In this study, we performed functional analyses of two homologous OsPUB2 and OsPUB3 in response to cold stress (4°C). OsPUB2 was up-regulated by high salinity, drought, and cold, whereas OsPUB3 was constitutively expressed. A subcellular localization assay revealed that OsPUB2 and OsPUB3 were localized to the exocyst positive organelle (EXPO)-like punctate structures...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28161604/-135-xe-measurements-with-a-two-element-czt-based-radioxenon-detector-for-nuclear-explosion-monitoring
#13
Lily Ranjbar, Abi T Farsoni, Eric M Becker
Measurement of elevated concentrations of xenon radioisotopes ((131m)Xe, (133m)Xe, (133)Xe and (135)Xe) in the atmosphere has been shown to be a very powerful method for verifying whether or not a detected explosion is nuclear in nature. These isotopes are among the few with enough mobility and with half-lives long enough to make their detection at long distances realistic. Existing radioxenon detection systems used by the Comprehensive Nuclear-Test-Ban Treaty Organization (CTBTO) suffer from problems such as complexity, need for high maintenance and memory effect...
February 2, 2017: Journal of Environmental Radioactivity
https://www.readbyqxmd.com/read/28153413/model-predicted-occurrence-of-multiple-pharmaceuticals-in-swedish-surface-waters-and-their-flushing-to-the-baltic-sea
#14
C Lindim, J van Gils, I T Cousins, R Kühne, D Georgieva, S Kutsarova, O Mekenyan
An exposure assessment for multiple pharmaceuticals in Swedish surface waters was made using the STREAM-EU model. Results indicate that Metformin (27 ton/y), Paracetamol (6.9 ton/y) and Ibuprofen (2.33 ton/y) were the drugs with higher amounts reaching the Baltic Sea in 2011. 35 of the studied substances had more than 1 kg/y of predicted flush to the sea. Exposure potential given by the ratio amount of the drug exported to the sea/amount emitted to the environment was higher than 50% for 7 drugs (Piperacillin, Lorazepam, Metformin, Hydroxycarbamide, Hydrochlorothiazide, Furosemide and Cetirizine), implying that a high proportion of them will reach the sea, and below 10% for 27 drugs, implying high catchment attenuation...
January 30, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28152573/survival-probabilities-of-end-stage-renal-disease-patients-on-renal-replacement-therapy-in-iran
#15
Alireza Heidary Rouchi, Mohammad Ali Mansournia, Mohammad Aghighi, Mitra Mahdavi-Mazdeh
BACKGROUND: To bridge the gap in the current knowledge, the present study was conducted to obtain evidences relating to clinical outcomes of the ESRD population in Iran over the last two decades. METHODS: The records of 84,652 incident ESRD patients from 1995 up to and including 2014 in the national registry of ESRD patients were analyzed retrospectively. Data were collected from dialysis and transplant centers in Iran from 1995 to 2008 via paper forms and from 2009 to 2014 through web-based records...
February 2, 2017: Nephrology
https://www.readbyqxmd.com/read/28150239/analysis-of-cysteine-redox-post-translational-modifications-in-cell-biology-and-drug-pharmacology
#16
Revati Wani, Brion W Murray
Reversible cysteine oxidation is an emerging class of protein post-translational modification (PTM) that regulates catalytic activity, modulates conformation, impacts protein-protein interactions, and affects subcellular trafficking of numerous proteins. Redox PTMs encompass a broad array of cysteine oxidation reactions with different half-lives, topographies, and reactivities such as S-glutathionylation and sulfoxidation. Recent studies from our group underscore the lesser known effect of redox protein modifications on drug binding...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28146090/immobilized%C3%A2-lipases%C3%A2-on%C3%A2-functionalized%C3%A2-silica-particles%C3%A2-as%C3%A2-potential%C3%A2-biocatalysts%C3%A2-for%C3%A2-the%C3%A2-synthesis%C3%A2-of%C3%A2-fructose%C3%A2-oleate%C3%A2-in%C3%A2-an%C3%A2-organic%C3%A2-solvent-water%C3%A2-system
#17
Vinicius Vescovi, Raquel L C Giordano, Adriano A Mendes, Paulo W Tardioli
Lipases from Thermomyces lanuginosus (TLL) and Pseudomonas fluorescens (PFL) wereimmobilized on functionalized silica particles aiming their use in the synthesis of fructose oleate in a tert-butyl alcohol/water system. Silica particles were chemically modified with octyl (OS), octyl plus glutaraldehyde (OSGlu), octyl plus glyoxyl(OSGlx), and octyl plus epoxy groups(OSEpx). PFL was hyperactivated on all functionalized supports (more than 100% recovered activity) using low protein loading (1 mg/g), however, for TLL, this phenomenon was observed only using octyl-silica (OS)...
January 30, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28143610/severe-toxicity-induced-by-accumulation-of-active-sunitinib-metabolite-in-a-japanese-patient-with-renal-cell-carcinoma-a-case-report
#18
Shinya Takasaki, Masafumi Kikuchi, Yoshihide Kawasaki, Akihiro Ito, Yoichi Arai, Hiroaki Yamaguchi, Nariyasu Mano
BACKGROUND: Sunitinib is a multi-targeted tyrosine kinase inhibitor that is approved for treatment of renal cell carcinoma as an oral anticancer drug. Therapeutic drug monitoring of total sunitinib (sunitinib and N-desethyl sunitinib) is used in our hospital to improve therapeutic efficacy, while preventing adverse effects. Here, we report the first case of a patient with metastatic renal cell carcinoma undergoing hemodialysis and presenting severe adverse events induced by the accumulation of N-desethyl sunitinib...
February 1, 2017: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/28138743/an-efficient-liquid-chromatography-high-resolution-mass-spectrometry-approach-for-the-optimization-of-the-metabolic-stability-of-therapeutic-peptides
#19
Simone Esposito, Riccardo Mele, Raffaele Ingenito, Elisabetta Bianchi, Fabio Bonelli, Edith Monteagudo, Laura Orsatti
In drug discovery, there is increasing interest in peptides as therapeutic agents due to several appealing characteristics that are typical of this class of compounds, including high target affinity, excellent selectivity, and low toxicity. However, peptides usually present also some challenging ADME (absorption, distribution, metabolism, and excretion) issues such as limited metabolic stability, poor oral bioavailability, and short half-lives. In this context, early preclinical in vitro studies such as plasma metabolic stability assays are crucial to improve developability of a peptidic drug...
January 30, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28138016/protein-degradation-rate-in-arabidopsis-thaliana-leaf-growth-and-development
#20
Lei Li, Clark J Nelson, Josua Trösch, Ian Castleden, Shaobai Huang, A Harvey Millar
We have applied 15N labeling approaches to leaves of the Arabidopsis thaliana rosette to characterize their protein degradation rate and understand its determinants. The progressive labeling of new peptides with 15N and measuring the decrease in the abundance of >60,000 existing peptides over time allowed us to define the degradation rate of 1228 proteins in vivo. We show that Arabidopsis protein half-lives vary from several hours to several months based on the exponential constant of the decay rate for each protein...
January 30, 2017: Plant Cell
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