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antibody phage display

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https://www.readbyqxmd.com/read/27888111/neutralizing-human-recombinant-antibodies-against-herpes-simplex-virus-type-1-glycoproteins-b-from-a-phage-displayed-scfv-antibody-library
#1
Vahid Bagheri, Foroogh Nejatollahi, Seyed Alireza Esmaeili, Amir Abbas Momtazi, Mohamad Motamedifar, Amirhossein Sahebkar
The HSV-1 envelope glycoprotein B (gB) plays a critical role in virus entry into host cells. Neutralizing antibodies can therefore potentially prevent virus entry into target cells and cell-to-cell spread of infection. Our present study focused on the selection of neutralizing single-chain Fv (scFv) antibodies of a phage-displayed nonimmune human scFv antibody library against gB of HSV-1. To enrich specific scFvs, two phage antibodies were isolated against amino acid residues 31-43 derived from the N-terminal part of gB using panning technique...
November 22, 2016: Life Sciences
https://www.readbyqxmd.com/read/27887869/a-highly-diverse-and-functional-na%C3%A3-ve-ubiquitin-variant-library-for-generation-of-intracellular-affinity-reagents
#2
Isabel Leung, Nick Jarvik, Sachdev Sidhu
We report the design, construction and validation of a highly diverse phage-displayed naïve ubiquitin variant (Ubv) library. We first conducted a mutation tolerance scan of 27 residues and confirmed that 24 of these could be substituted by chemically diverse amino acids without compromising the display of Ubvs on phage. Subsequently, we constructed a library containing 6.8×10(10) unique members in which these 24 positions were diversified with a degenerate codon that encodes for six amino acids that are prevalent in protein interaction sites...
November 22, 2016: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27874102/development-and-comparative-study-of-chemosynthesized-antigen-and-mimotope-based-immunoassays-for-class-specific-analysis-of-o-o-dimethyl-organophosphorus-pesticides
#3
Fengchun Zhao, Huimin Wang, Xiao Han, Zhengyou Yang
The multi-residue determination of organophosphorus pesticides (OPs) is an important task due to the wide application and high toxicity of OPs. However, there is no promising immunoassay to monitor the multi-residue of O,O-dimethyl OPs. In this study, a monoclonal antibody (mAb) against a generic hapten of O,O-dimethyl OPs (O,O-dimethyl O-(3-carboxyphenyl)phosphorothioate) was prepared. To develop an effective class-specific immunoassay, two strategies were performed to select the appropriate coating antigen or competing antigen...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27863546/identification-of-three-novel-b-cell-epitopes-of-vmh-protein-from-vibrio-mimicus-by-screening-a-phage-display-peptide-library
#4
Ning Xiao, Ji Cao, Hao Zhou, Shu-Quan Ding, Ling-Yan Kong, Jin-Nian Li
Vibrio mimicus is the causative agent of ascites disease in fish. The heat-labile hemolytic toxin designated VMH is an immunoprotective antigen of V. mimicus. However, its epitopes have not been well characterized. Here, a commercially available phage displayed 12-mer peptide library was used to screen epitopes of VMH protein using polyclonal rabbit anti-rVMH protein antibodies, and then five positive phage clones were identified by sandwich and competitive ELISA. Sequences analysis showed that the motif of DPTLL displayed on phage clone 15 and the consensus motif of SLDDDST displayed on the clone 4/11 corresponded to the residues 134-138 and 238-244 of VMH protein, respectively, and the synthetic motif peptides could also be recognized by anti-rVMH-HD antibody in peptide-ELISA...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27863402/a-fully-human-anti-cd47-blocking-antibody-with-therapeutic-potential-for-cancer
#5
Dadi Zeng, Qiang Sun, Ang Chen, Jiangfeng Fan, Xiaopeng Yang, Lei Xu, Peng Du, Weiyi Qiu, Weicai Zhang, Shuang Wang, Zhiwei Sun
CD47/SIRPα interaction serves as an immune checkpoint for macrophage-mediated phagocytosis. Mouse anti-CD47 blocking antibodies had demonstrated potent efficacy in the treatment of both leukemic and solid tumors in preclinical experimentations, and therefore had moved forward rapidly into clinical trials. However, a fully human blocking antibody, which meets clinical purpose better, has not been reported for CD47 up to date. In this study, we reported the isolation of a fully human anti-CD47 blocking antibody, ZF1, from a phage display library...
November 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27856318/sympathetic-nerve-repulsion-inhibited-by-designer-molecules-in-vitro-and-role-in-experimental-arthritis
#6
Julia Kunath, Nicolas Delaroque, Michael Szardenings, Ines Neundorf, Rainer H Straub
AIMS: In rheumatoid arthritis and collagen type II arthritis (CIA), sympathetic nerve fibers get lost in inflamed tissue. The process is probably induced by nerve repellent factors like semaphorin 3F (SEMA3F). Repulsion of sympathetic nerve fibers in inflamed tissue has proinflammatory effects due to the loss of anti-inflammatory neurotransmitters. We hypothesized that design molecules like antibodies and specific peptides that inhibit nerve fiber repulsion can ameliorate CIA. MATERIALS AND METHODS: Two blocking antibodies were used and four blocking peptides were generated using the phage display technique with the targets of SEMA3F and plexin-A2...
November 14, 2016: Life Sciences
https://www.readbyqxmd.com/read/27849134/developing-and-characterization-of-single-chain-variable-fragment-scfv-antibody-against-frizzled-7-fzd7-receptor
#7
Hamid Nickho, Vahid Younesi, Leili Aghebati-Maleki, Morteza Motallebnezhad, Jafar Majidi Zolbanin, Aliakbar Movassagh Pour, Mehdi Yousefi
Wnt/β-catenin signaling pathway through Frizzled receptors has been shown to play a key role in both normal development and tumorigenesis. Overexpression of Wnt pathway genes, such as Fzd7 in several malignancies is well-documented. Therefore, targeting of Fzd7 and its ligand inhibits cancer cells proliferation metastasis. In the present study we isolated single chain variable fragments (scFvs) against Fzd7 receptor using phage display method. Semi-synthetic human naive antibody libraries (Tomlinson I + J) was employed in panning procedure to isolate specific scFv against specific peptide from extracellular domain of Fzd7 receptor...
November 16, 2016: Bioengineered
https://www.readbyqxmd.com/read/27845058/identification-of-the-immunogenic-epitopes-of-the-whole-venom-component-of-the-hemiscorpius-lepturus-scorpion-using-the-phage-display-peptide-library
#8
Roghaye Jahdasani, Fatemeh Rahimi Jamnani, Mahdi Behdani, Mahdi Habibi-Anbouhi, Najmeh Yardehnavi, Delavar Shahbazzadeh, Fatemeh Kazemi-Lomedasht
The venom of the Hemiscorpius lepturus scorpion contains mixtures of bioactive compounds that disturb biochemical and physiological functions of the victims. Hemiscorpius lepturus envenomation is recognized as a serious health concern in tropical regions. So far, there is no preventive procedure, and the main focus is on treatment of victims with an antiserum purified from hyper-immunized horses. Although antisera can neutralize the venom, they, in some cases, lead to anaphylactic shock and even death. Selection of peptides mimicking antigenic and immunogenic epitopes of toxins from random peptide libraries is a novel approach for the development of recombinant toxins and poly-epitopic vaccine...
December 15, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27845043/molecular-characterization-of-a-single-chain-antibody-variable-fragment-scfv-specific-for-pspa-from-streptococcus-pneumoniae
#9
ShinA Jang, GyuHee Kim, Jee Hye Oh, Seung Yeob Rhee, Dongho Kim, Kook Han Kim, Yong Ho Kim, Dong-Kwon Rhee, Sangho Lee
Streptococcus pneumoniae is a major infectious agent responsible for pneumonia, otitis media, sepsis and meningitis. Pneumococcal surface protein A (PspA) is a well-characterized virulence factor localized on the surface and a target for vaccine development. In this study, we screened a single-chain antibody variable fragment (scFv) using phage display from a human synthetic library to select a clone 2B11. Affinity (Kd) of 2B11 was measured to be 5 nM using biolayer interferometry. 2B11 exhibited a dose-dependent recognition of recombinant PspA with no cross-reactivity towards pneumococcal antigens...
November 11, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27834908/epitope-identification-and-application-for-diagnosis-of-duck-tembusu-virus-infections-in-ducks
#10
Chenxi Li, Junyan Liu, Wulin Shaozhou, Xiaofei Bai, Qingshan Zhang, Ronghong Hua, Jyung-Hurng Liu, Ming Liu, Yun Zhang
Duck Tembusu virus (DTMUV) causes substantial egg drop disease. DTMUV was first identified in China and rapidly spread to Malaysia and Thailand. The antigenicity of the DTMUV E protein has not yet been characterized. Here, we investigated antigenic sites on the E protein using the non-neutralizing monoclonal antibodies (mAbs) 1F3 and 1A5. Two minimal epitopes were mapped to (221)LD/NLPW(225) and (87)YAEYI(91) by using phage display and mutagenesis. DTMUV-positive duck sera reacted with the epitopes, thus indicating the importance of the minimal amino acids of the epitopes for antibody-epitope binding...
November 10, 2016: Viruses
https://www.readbyqxmd.com/read/27825348/bacterial-superglue-generates-a-full-length-circumsporozoite-protein-virus-like-particle-vaccine-capable-of-inducing-high-and-durable-antibody-responses
#11
Christoph M Janitzek, Sungwa Matondo, Susan Thrane, Morten A Nielsen, Reginald Kavishe, Steve B Mwakalinga, Thor G Theander, Ali Salanti, Adam F Sander
BACKGROUND: Malaria, caused by Plasmodium falciparum, continues to have a devastating impact on global health, emphasizing the great need for a malaria vaccine. The circumsporozoite protein (CSP) is an attractive target for a malaria vaccine, and forms a major component of RTS,S, the most clinically advanced malaria vaccine. The clinical efficacy of RTS,S has been moderate, yet has demonstrated the viability of a CSP-based malaria vaccine. In this study, a vaccine comprised of the full-length CSP antigen presented on a virus-like particle (VLP) is produced using a split-intein conjugation system (SpyTag/SpyCatcher) and the immunogenicity is tested in mice...
November 8, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27824100/identification-of-a-new-broadly-cross-reactive-epitope-within-domain-iii-of-the-duck-tembusu-virus-e-protein
#12
Chenxi Li, Xiaofei Bai, Runze Meng, Wulin Shaozhou, Qingshan Zhang, Ronghong Hua, Jyung-Hurng Liu, Ming Liu, Yun Zhang
In 2010, a pathogenic flavivirus termed duck Tembusu virus (DTMUV) caused widespread outbreak of egg-drop syndrome in domesticated ducks in China. Although the glycoprotein E of DTMUV is an important structural component of the virus, the B-cell epitopes of this protein remains uncharacterized. Using phage display and mutagenesis, we identified a minimal B-cell epitope, (374)EXE/DPPFG(380), that mediates binding to a nonneutralizing monoclonal antibody. DTMUV-positive duck serum reacted with the epitope, and amino acid substitutions revealed the specific amino acids that are essential for antibody binding...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27819143/evolution-of-phage-display-technology-from-discovery-to-application
#13
Leila Rahbarnia, Safar Farajnia, Hossein Babaei, Jafar Majidi, Kamal Veisi, Vahideh Ahmadzadeh, Bahman Akbari
Phage display technology as a selection-based system is an attractive method for evolution of new biological drugs. Unique ability of phage libraries for displaying proteins on bacteriophage surfaces enable them to make a major contribution in diverse fields of researches related to the diagnosis and therapy of diseases. One of the great challenges facing researchers is the modification of phage display technology and the development of new applications. This article reviews the molecular basis of phage display library, and summarizes the novel and specific applications of this technique in the field of biological drugs development including therapeutic antibodies, peptides, vaccines, and catalytic antibodies...
November 21, 2016: Journal of Drug Targeting
https://www.readbyqxmd.com/read/27805806/protein-binders-specific-for-immunoglobulin-g-from-different-species-for-immunoassays-and-multiplex-imaging
#14
Sukyo Jeong, Woosung Heu, Jong-Won Kim, Hak-Sung Kim
An immunoassay is the most widely used method for analyzing a variety of analytes based on antigen-antibody interactions in the biological and medical sciences. However, the use of secondary antibodies has certain shortcomings, such as a high cost, cross-reactivity, and loss of binding affinity during labelling. Herein, we present the development of repebodies specifically binding to immunoglobulin G with a different origin, which is a small-sized non-antibody scaffold composed of leucine-rich repeat (LRR) modules, for use in immunoassays and imaging...
November 2, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27798615/human-antibody-repertoire-after-vsv-ebola-vaccination-identifies-novel-targets-and-virus-neutralizing-igm-antibodies
#15
Surender Khurana, Sandra Fuentes, Elizabeth M Coyle, Supriya Ravichandran, Richard T Davey, John H Beigel
Development of an effective vaccine against Ebola virus is of high priority. However, knowledge about potential correlates of protection and the durability of immune response after vaccination is limited. Here, we elucidate the human antibody repertoire after administration of vesicular stomatitis virus (VSV)-Ebola vaccine at 3 million, 20 million and 100 million plaque-forming units (PFU) and homologous VSV-Ebola vaccine boost in healthy adult volunteers. Whole genome-fragment phage display libraries, expressing linear and conformational epitopes of Ebola glycoprotein (GP), showed higher diversity of antibody epitopes in individuals vaccinated with 20 million PFU than in those vaccinated with 3 million or 100 million PFU...
October 31, 2016: Nature Medicine
https://www.readbyqxmd.com/read/27792006/construction-of-human-anti-tetanus-single-chain-variable-fragment-applying-symplex-technology
#16
Laya Esmati, Jalil Fallah Mehrabadi, Masoumeh Bazaz, Hamideh Rouhani Nejad
BACKGROUND: Human monoclonal antibodies are important molecules in clinical research. Current Limitations of mAb technologies namely instability of immortalized B-cell line and probability of forming unusual VH-VL pairs in phage-display method led to mAbs technology based on single plasma cell called ``SYMPLEX''. OBJECTIVE: In this method, cognate VH and VL fragments generated from individual antibody genes exactly the same as natural ones. METHODS: PBMCs of whole blood of an immunized candidate was used as a resource of rearranged Ab genes...
October 20, 2016: Human Antibodies
https://www.readbyqxmd.com/read/27791177/towards-a-transcriptome-based-theranostic-platform-for-unfavorable-breast-cancer-phenotypes
#17
Andrey S Dobroff, Sara D'Angelo, Bedrich L Eckhardt, Fortunato Ferrara, Daniela I Staquicini, Marina Cardó-Vila, Fernanda I Staquicini, Diana N Nunes, Kisu Kim, Wouter H P Driessen, Amin Hajitou, Lesley C Lomo, Marc Barry, Savitri Krishnamurthy, Aysegul Sahin, Wendy A Woodward, Eric R Prossnitz, Robin L Anderson, Emmanuel Dias-Neto, Ursa A Brown-Glaberman, Melanie E Royce, Naoto T Ueno, Massimo Cristofanilli, Gabriel N Hortobagyi, Serena Marchiò, Juri G Gelovani, Richard L Sidman, Wadih Arap, Renata Pasqualini
Inflammatory breast carcinoma (IBC) is one of the most lethal forms of human breast cancer, and effective treatment for IBC is an unmet clinical need in contemporary oncology. Tumor-targeted theranostic approaches are emerging in precision medicine, but only a few specific biomarkers are available. Here we report up-regulation of the 78-kDa glucose-regulated protein (GRP78) in two independent discovery and validation sets of specimens derived from IBC patients, suggesting translational promise for clinical applications...
October 24, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27786270/recombinant-antibodies-for-specific-detection-of-clostridial-fe-fe-hydrogenases
#18
Rahul Mangayil, Matti Karp, Urpo Lamminmäki, Ville Santala
Biological hydrogen production is based on activity of specific enzymes called hydrogenases. Hydrogenases are oxygen sensitive metalloenzymes containing Ni and/or Fe atoms at the active site, catalyzing reversible reduction of protons. Generally, [Fe-Fe] hydrogenases prefer proton reduction to molecular hydrogen, a potential energy carrier molecule that can be produced by bioprocesses in sustainable manner. Thus, monitoring tools have been developed to study the relationship between [Fe-Fe] hydrogenases and biohydrogen production in bioreactors at DNA and RNA levels...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27782255/systemic-lupus-erythematosus-molecular-cloning-and-analysis-of-recombinant-monoclonal-kappa-light-chain-ngta1-me-pro-with-two-metalloprotease-active-centers
#19
Anna M Timofeeva, Valentina N Buneva, Georgy A Nevinsky
It was shown previously that approximately 30% ± 5% of antibodies against myelin basic protein (MBP) and the DNA of patients with systemic lupus erythematosus (SLE) and multiple sclerosis (MS) possess catalytic activities that play an important negative role in the pathogenesis of MS and SLE. An immunoglobulin light chain phagemid library derived from peripheral blood lymphocytes of patients with SLE was used. The small pools of phage particles displaying light chains with different affinity for MBP were isolated by affinity chromatography on MBP-Sepharose, and the fraction eluted with 0...
October 26, 2016: Molecular BioSystems
https://www.readbyqxmd.com/read/27781936/editorial-antibody-phage-display-the-evolution-of-antibody-therapeutics
#20
Theam Soon Lim
No abstract text is available yet for this article.
October 19, 2016: Current Pharmaceutical Design
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