keyword
MENU ▼
Read by QxMD icon Read
search

antibody phage display

keyword
https://www.readbyqxmd.com/read/29242049/rational-library-design-by-functional-cdr-resampling
#1
Qi Zhao, Diane Buhr, Courtney Gunter, Jenny Frenette, Mary Ferguson, Eric Sanford, Erika Holland, Chitra Rajagopal, Melissa Batonick, Margaret M Kiss, Michael P Weiner
Successful antibody discovery relies on diversified libraries, where two aspects are implied, namely the absolute number of unique clones and the percentage of functional clones. Instead of pursuing the absolute quantity thresholded by current display technology, we have sought to maximize the effective diversity by improving functional clone percentage. With the combined effort of bioinformatics, structural biology, molecular immunology and phage display technology, we devised a bioinformatic pipeline to construct and validate libraries via combinatorial assembly of sequences from a database of experimentally validated antibodies...
December 11, 2017: New Biotechnology
https://www.readbyqxmd.com/read/29240734/transient-expression-of-human-antibodies-in-mammalian-cells
#2
Rodrigo Vazquez-Lombardi, Damien Nevoltris, Ansha Luthra, Peter Schofield, Carsten Zimmermann, Daniel Christ
Recombinant expression of antibody molecules in mammalian cells offers important advantages over traditionally utilized bacterial expression, including glycosylation required for antibody functionality and markedly reduced levels of endotoxin contamination. Advances in transient mammalian expression systems enable high yields (>100 mg/liter) that now allow for effective recombinant antibody production at a reasonable cost. Here, we provide step-by-step protocols for the design and recombinant expression of full-length IgG antibodies and antibody-derived constructs (including Fab, Fc-fusions and bispecifics) in mammalian cells...
January 2018: Nature Protocols
https://www.readbyqxmd.com/read/29238460/synthetic-peptides-mimicking-lipopolysaccharide-as-a-potential-vaccine-candidates-against-vibrio-cholerae-serogroup-o1
#3
Fatemeh Mohammad Pour Ghazi, Seyed Latif Mousavi Gargari
Background and Objectives: Cholera is a life-threatening diarrhea caused mainly by Gram-negative marine habitant Vibrio cholerae serogroup O1. Cholera vaccination is limited mainly to developed countries, due to the cumbersome and expensive task of vaccine production. In the present work, the aim was to study the immunogenicity of the synthetic mimotopes through two different routes of injection and oral administration. Lipopolysaccharide (LPS) is one of the immunogenic components in Gram-negative bacteria, which cannot be used as a vaccine candidate, due to its high toxic effect...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29237366/antigen-antibody-interaction-based-self-healing-capability-of-hybrid-hydrogels-composed-of-genetically-engineered-filamentous-viruses-and-gold-nanoparticles
#4
Toshiki Sawada, Takeshi Serizawa
M13 phages, a type of filamentous virus, have recently been utilized as components for developing novel functional soft materials in various fields. Herein, we demonstrated the self-healing capability of hybrid hydrogels composed of antigen peptide-displaying M13 phages and antibody-immobilized gold nanoparticles (GNPs). The addition of small amounts of buffer solutions to the rupture points of the hybrid hydrogels followed by incubation resulted in self-healing, possibly due to specific interactions between the components...
December 13, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29233140/selection-characterization-and-thermal-stabilization-of-llama-single-domain-antibodies-towards-ebola-virus-glycoprotein
#5
Jinny L Liu, Lisa C Shriver-Lake, George P Anderson, Dan Zabetakis, Ellen R Goldman
BACKGROUND: A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for Ebola virus envelope glycoprotein (GP) and increased their stability to expand their utility for use in austere locals...
December 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29218233/targeted-delivery-of-sirna-therapeutics-to-malignant-tumors
#6
REVIEW
Qixin Leng, Martin C Woodle, A James Mixson
Over the past 20 years, a diverse group of ligands targeting surface biomarkers or receptors has been identified with several investigated to target siRNA to tumors. Many approaches to developing tumor-homing peptides, RNA and DNA aptamers, and single-chain variable fragment antibodies by using phage display, in vitro evolution, and recombinant antibody methods could not have been imagined by researchers in the 1980s. Despite these many scientific advances, there is no reason to expect that the ligand field will not continue to evolve...
2017: Journal of Drug Delivery
https://www.readbyqxmd.com/read/29207143/screening-and-antitumor-effect-of-an-anti%C3%A2-ctla%C3%A2-4-nanobody
#7
Ruirong Wan, Aiqun Liu, Xiaoqiong Hou, Zongqiang Lai, Jieping Li, Nuo Yang, Juntao Tan, Fengzhen Mo, Zixi Hu, Xiaomei Yang, Yongxiang Zhao, Xiaoling Lu
Cytotoxic T‑lymphocyte antigen‑4 (CTLA‑4) is a critical negative regulator of immune responses. CTLA‑4 is rapidly upregulated following T‑cell activation, and then binds to B7 molecules with a higher affinity than CD28. CTLA‑4 may abolish the initiation of the responses of T cells by raising the threshold of signals required for full activation of T cells, and it also may terminate ongoing T-cell responses. This regulatory role has led to the development of monoclonal antibodies (mAbs) designed to block CTLA‑4 activity for enhancing immune responses against cancer...
December 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29199242/a-single-step-breeding-generated-a-diagnostic-anti-cortisol-antibody-fragment-with-over-30-fold-enhanced-affinity
#8
Hiroyuki Oyama, Izumi Morita, Yuki Kiguchi, Tomomi Morishita, Sakiko Fukushima, Yuki Nishimori, Toshifumi Niwa, Norihiro Kobayashi
Cortisol levels in bodily fluids represent a useful index for pituitary-adrenal function, and thus practical anti-cortisol antibodies are required. We have studied "antibody-breeding" approaches, which involve in vitro evolution of antibodies to improve their antigen-binding performances. Here, we produced an antibody fragment to measure serum cortisol levels with over 30-fold enhanced affinity after single mutagenesis and selection steps. A mouse anti-cortisol antibody, Ab-CS#3, with insufficient affinity for practical use, was chosen as the prototype antibody...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29182455/isolation-of-blood-brain-barrier-crossing-antibodies-from-a-phage-display-library-by-competitive-elution-and-their-ability-to-penetrate-the-central-nervous-system
#9
George Thom, Jon Hatcher, Arron Hearn, Judy Paterson, Natalia Rodrigo, Arthur Beljeana Ian Gurrell, Carl Webster
The blood-brain barrier (BBB) is a formidable obstacle for delivery of biologic therapeutics to central nervous system (CNS) targets. Whilst the BBB prevents passage of the vast majority of molecules, it also selectively transports a wide variety of molecules required to maintain brain homeostasis. Receptor-mediated transcytosis is one example of a macromolecule transport system that is employed by cells of the BBB to supply essential proteins to the brain and which can be utilized to deliver biologic payloads, such as antibodies, across the BBB...
November 28, 2017: MAbs
https://www.readbyqxmd.com/read/29181008/engineered-soluble-monomeric-igg1-fc-with-significantly-decreased-non-specific-binding
#10
Chunyu Wang, Yanling Wu, Lili Wang, Binbin Hong, Yujia Jin, Dan Hu, Gang Chen, Yu Kong, Ailing Huang, Guoqiang Hua, Tianlei Ying
Due to the long serum half-life provided by the neonatal Fc receptor (FcRn) recycling, the IgG1 Fc has been pursued as the fusion partner to develop therapeutic Fc-fusion proteins, or as the antibody-derived scaffold that could be engineered with antigen-binding capabilities. In previous studies, we engineered the monomeric Fc by mutating critical residues located on the IgG1 Fc dimerization interface. Comparing with the wild-type dimeric Fc, monomeric Fc might possess substantial advantages conferred by its smaller size, but also suffers the disadvantage of non-specific binding to some unrelated antigens, raising considerable concerns over its potential clinical development...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29175317/epitope-identification-of-specific-naturally-occurring-human-anti-avidin-antibodies
#11
Boris Tchernychev, Talia Miron, Meir Wilchek
Human serum contains natural antibodies against avidin. Affinity purified natural anti-avidin human IgG exhibits affinity constants comparable to those of antibodies produced by active immunization of rabbits. Using a random hexapeptide library displayed on the filamentous M13 phage, and rabbit anti-avidin purified antibodies as a selector, we searched for epitopes shared by both selector and natural human anti-avidin IgG. This approach, enabled the isolation and identification of phagotopes bearing consensus motifs similar to sequence stretches of the avidin loops and β-sheet regions...
November 22, 2017: Immunology Letters
https://www.readbyqxmd.com/read/29168727/a-supercluster-of-neutralizing-epitopes-at-the-interface-of-ricin-s-enzymatic-rta-and-binding-rtb-subunits
#12
Amanda Y Poon, David J Vance, Yinghui Rong, Dylan Ehrbar, Nicholas J Mantis
As part of an effort to engineer ricin antitoxins and immunotherapies, we previously produced and characterized a collection of phage-displayed, heavy chain-only antibodies (VHHs) from alpacas that had been immunized with ricin antigens. In our initial screens, we identified nine VHHs directed against ricin toxin's binding subunit (RTB), but only one, JIZ-B7, had toxin-neutralizing activity. Linking JIZ-B7 to different VHHs against ricin's enzymatic subunit (RTA) resulted in several bispecific antibodies with potent toxin-neutralizing activity in vitro and in vivo...
November 23, 2017: Toxins
https://www.readbyqxmd.com/read/29165857/isolation-of-phage-display-library-derived-scfv-antibody-specific-to-listeria-monocytogenes-by-a-novel-immobilized-method
#13
Nguyen Xuan-Hung, Thu-Le Trinh, Huong Thi-Bich Vu, Huan Quang Le, Anh To Kim
AIMS: To select Listeria monocytogenes specific scFv antibodies from a phage display library by a novel simple and cost-effective immobilization method. METHODS AND RESULTS: Light Expanded Clay Aggregate (LECA) was used as biomass support matrix for biopanning of a phage display library to select Listeria monocytogenes specific scFv antibody. Four rounds of positive selection against LECA-immobilized L. monocytogenes and an additional subtractive panning against L...
November 22, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29165352/antibody-based-protective-immunity-against-helminth-infections-antibody-phage-display-derived-antibodies-against-bmr1-antigen
#14
Anizah Rahumatullah, Izzati Zahidah Abdul Karim, Rahmah Noordin, Theam Soon Lim
Helminth parasite infections are significantly impacting global health, with more than two billion infections worldwide with a high morbidity rate. The complex life cycle of the nematodes has made host immune response studies against these parasites extremely difficult. In this study, we utilized two phage antibody libraries; the immune and naïve library were used to identify single chain fragment variable (scFv) clones against a specific filarial antigen (BmR1). The V-gene analysis of isolated scFv clones will help shed light on preferential VDJ gene segment usage against the filarial BmR1 antigen in healthy and infected states...
November 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29163485/a-novel-affinity-tag-abtag-and-its-application-to-the-affinity-screening-of-single-domain-antibodies-selected-by-phage-display
#15
Greg Hussack, Toya Nath Baral, Jason Baardsnes, Henk van Faassen, Shalini Raphael, Kevin A Henry, Jianbing Zhang, C Roger MacKenzie
ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reduction in the capacity of the BSA surfaces to bind ABTAG. A moderate throughput, SPR-based, antibody screening assay exploiting the unique features of ABTAG is described. Anti-carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) sdAbs were isolated from a phage-displayed sdAb library derived from the heavy chain antibody repertoire of a llama immunized with CEACAM6...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29162919/production-of-recombinant-human-procollagen-type-i-c-terminal-propeptide-and-establishment-of-a-sandwich-elisa-for-quantification
#16
Woo-Young Seo, Jeong-Ho Kim, Du-San Baek, Su-Jung Kim, Sujin Kang, Won Suk Yang, Ji-Ae Song, Moo-Seung Lee, Sunghoon Kim, Yong-Sung Kim
Procollagen type I carboxy-terminal propeptide (PICP), derived from type I procollagen, has been identified as an indicator of type I collagen synthesis in bone matrix formation and skin recovery. PICP is a heterotrimeric glycoprotein consisting of two α1 chains (PICPα1) and one α2 chain (PICPα2). Here, we report the recombinant expression of human PICP using a mammalian expression system. Co-expression of PICPα1 and PICPα2 in HEK293F cells resulted in the production of functional PICP in the correctly assembled heterotrimeric form...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29159596/human-monoclonal-antibodies-as-candidate-therapeutics-against-emerging-viruses
#17
REVIEW
Yujia Jin, Cheng Lei, Dan Hu, Dimiter S Dimitrov, Tianlei Ying
The emergence of new pathogens, such as severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), and Ebola virus, poses serious challenges to global public health and highlights the urgent need for novel antiviral approaches. Monoclonal antibodies (mAbs) have been successfully used to treat various diseases, particularly cancer and immunological disorders. Antigen-specific mAbs have been isolated using several different approaches, including hybridoma, transgenic mice, phage display, yeast display, and single B-cell isolation...
November 20, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/29152066/identification-and-validation-of-fgfr2-peptide-for-detection-of-early-barrett-s-neoplasia
#18
Juan Zhou, Lei He, Zhijun Pang, Henry D Appelman, Rork Kuick, David G Beer, Meng Li, Thomas D Wang
The incidence of esophageal adenocarcinoma (EAC) is rising rapidly, and early detection within the precursor state of Barrett's esophagus (BE) is challenged by flat premalignant lesions that are difficult detect with conventional endoscopic surveillance. Overexpression of cell surface fibroblast growth factor receptor 2 (FGFR2) is an early event in progression of BE to EAC, and is a promising imaging target. We used phage display to identify the peptide SRRPASFRTARE that binds specifically to the extracellular domain of FGFR2...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29138294/isolation-of-isoform-specific-binding-proteins-affimers-by-phage-display-using-negative-selection
#19
Anna Ah-San Tang, Christian Tiede, David J Hughes, Michael J McPherson, Darren C Tomlinson
Some 30 years after its discovery, phage display remains one of the most widely used methods of in vitro selection. Initially developed to revolutionize the generation of therapeutic antibodies, phage display is now the first choice for screening artificial binding proteins. Artificial binding proteins can be used as reagents to study protein-protein interactions, target posttranslational modifications, and distinguish between homologous proteins. They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics...
November 14, 2017: Science Signaling
https://www.readbyqxmd.com/read/29130184/generation-of-high-sensitivity-monoclonal-antibodies-specific-for-homoserine-lactones
#20
Soumya Palliyil
A number of bacteria use a class of chemical compounds called acyl-homoserine lactones (AHLs) as quorum sensing (QS) signals to coordinate their behavior at the population level, including pathogens like Pseudomonas aeruginosa. Blocking QS using antibodies is an attractive strategy for infection control as this process takes a central role in P. aeruginosa infections. Here the methods involved in the generation of high sensitivity anti-QS monoclonal antibodies from an immunized sheep phage display antibody library are described...
2018: Methods in Molecular Biology
keyword
keyword
91310
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"