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https://www.readbyqxmd.com/read/28078413/establishment-of-a-sandwich-enzyme-linked-immunosorbent-assay-for-specific-detection-of-bacillus-thuringiensis-bt-cry1ab-toxin-utilizing-a-monoclonal-antibody-produced-with-a-novel-hapten-designed-with-molecular-model
#1
Sa Dong, Xiao Zhang, Yuan Liu, Cunzheng Zhang, Yajing Xie, Jianfeng Zhong, Chongxin Xu, Xianjin Liu
Cry1Ab toxin is commonly expressed in genetically modified crops in order to control chewing pests. At present, the detection method with enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibody cannot specifically detect Cry1Ab toxin for Cry1Ab's amino acid sequence and spatial structure are highly similar to Cry1Ac toxin. In this study, based on molecular design, a novel hapten polypeptide was synthesized and conjugated to keyhole limpet hemocyanin (KLH). Then, through animal immunization with this antigen, a monoclonal antibody named 2C12, showing high affinity to Cry1Ab and having no cross reaction with Cry1Ac, was produced...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28072740/keyhole-approach-in-the-neuroendoscopic-treatment-for-hydrocephalus
#2
Qiang Cai, Xiangyang Zhang, Long Wang, Shulan Huang, Zhibiao Chen, Qianxue Chen
The aim of the study was to explore keyhole approach and dura suture in the neuroendoscopic treatment for hydrocephalus.Twelve cases of hydrocephalus patients who were treated with neuroendoscope by this approach were analyzed retrospectively from April 2015 to April 2016 in our department. The basic steps of this procedure was using drill and milling cutter to form a small bone flap instead of burr hole, and then making a cruciate incision on the dura. After endoscopic third ventriculostomy or endoscopic third ventriculostomy + ventriculocystostomy finished, dura was sutured and the bone flap was reset...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28070819/inducing-polyclonal-eag1-specific-antibodies-by-vaccination-with-a-linear-epitope-immunogen-and-its-relation-to-breast-tumorigenesis
#3
Zhandong Li, Ketong Zhu, Xin Gong, Steven Vasilescu, Yu Sun, Kaiqing Hong, Hao Li, Lin Li, Yaming Shan
Ether à-go-go 1 (KCNH1, Kv10.1) (Eag1) is a voltage-gated potassium channel, which is commonly overexpressed in tested breast cancer patients. This occurrence makes it a potential molecular marker and a promising tool for breast cancer diagnosis and therapy. In order to explore protective or specific polyclonal antibodies for further research, potential linear epitopes from Eag1 were collected by sequence alignment. The sequence was synthesized and then coupled to the carrier protein keyhole limpet hemocyanin (KLH) for animal immunization...
January 9, 2017: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/28059648/validation-of-effectiveness-of-keyhole-clipping-in-nonfrail-elderly-patients-with-unruptured-intracranial-aneurysms
#4
Kentaro Mori, Kojiro Wada, Naoki Otani, Arata Tomiyama, Terushige Toyooka, Kazuya Fujii, Kosuke Kumagai, Satoru Takeuchi, Satoshi Tomura, Takuji Yamamoto, Yasuaki Nakao, Hajime Arai
OBJECTIVE Advanced age is known to be associated with a poor prognosis after surgical clipping of unruptured intracranial aneurysms (UIAs). Keyhole clipping techniques have been introduced for less invasive treatment of UIAs. In this study, the authors compared the complications and clinical and radiological outcomes after keyhole clipping between nonfrail elderly patients (≥ 70 years) and nonelderly patients. METHODS Keyhole clipping (either supraorbital or pterional) was performed to treat 260 cases of relatively small (≤ 10 mm) anterior circulation UIAs...
January 6, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28047532/th-ef-bra-05-a-method%C3%A3-of-near-real-time-4d-mri-using-volumetric-dynamic-keyhole-vdk-in-the-presence-of-respiratory-motion-for-mr-guided-radiotherapy
#5
B Lewis, S Kim, T Kim
PURPOSE: To develop a novel method that enables 4D MR imaging in near real-time for continuous monitoring of tumor motion in MR-guided radiotherapy. METHODS: This method is mainly based on an idea of expanding dynamic keyhole to full volumetric imaging acquisition. In the VDK approach introduced in this study, a library of peripheral volumetric k-space data is generated in given number of phases (5 and 10 in this study) in advance. For 4D MRI at any given time, only volumetric central k-space data are acquired in real-time and combined with pre-acquired peripheral volumetric k-space data in the library corresponding to the respiratory phase (or amplitude)...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28042017/-the-extended-lateral-supraorbital-approach-and-extradural-anterior-clinoidectomy-through-a-fronto-pterio-orbital-window-technical-note-and-pilot-surgical-series
#6
Hugo Andrade-Barazarte, Max Jägersberg, Sirajeddin Belkhair, Rachel Tymianski, Mazda K Turel, Karl Schaller, Juha A Hernesniemi, Michael Tymianski, Ivan Radovanovic
BACKGROUND: Lateral approaches to treat anterior cranial fossa lesions have evolved since the first frontotemporal approach described by Dandy in 1918 OBJECTIVE: To describe a less invasive approach to perform an extradural anterior clinoidectomy (EAC) through a lateral supraorbital approach (LSO) for anterior circulation aneurysms and antero-lateral skull base lesions METHODS: The extended LSO involves performing a standard lateral supraorbital craniotomy, followed by drilling of the sphenoid wing and the lateral wall of the orbit through the frontal bony opening of the LSO without any temporal extension of the craniotomy...
December 29, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/28027189/a-new-method-of-fixation-with-the-yang-s-keyhole-plate-system-for-the-treatment-of-mandible-fractures
#7
In-Hee Woo, Ju-Won Kim, Jwa-Young Kim, Byoung-Eun Yang
PURPOSE: This study was designed to assess the clinical outcomes and surgical convenience of a newly designed Yang's Keyhole plate (YK plate) system for treatment of subcondyle and angle fractures of the mandible. To evaluate the system's clinical outcomes and surgical convenience, chart review and surgical simulation using a mandible replica model were performed. METHODS: The YK plate system, which is a slightly modified sliding plate, has 0.8-mm thick titanium and a widened hole in the anterior region to permit a screw head to be inserted...
December 23, 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/28005775/fully-endoscope-controlled-clipping-bilateral-middle-cerebral-artery-aneurysm-via-unilateral-supraorbital-keyhole-approach
#8
Jian-Peng Wang, Ze-Yu Wu, Jian Xu, Yi-He Dou
Clipping bilateral middle cerebral artery (bMCA) aneurysms via unilateral approach in a single-stage operation is considered as a challenge procedure. To our knowledge, there is no study in surgical management of patients with bMCA aneurysms by fully endoscope-controlled techniques. The author reported a patient with bMCA aneurysms who underwent aneurysms clipping via a unilateral supraorbital keyhole approach by endoscope-controlled microneurosurgery, and the patient had an uneventful postoperative course without neurologic impairment and complication...
November 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27989977/a-review-of-the-literature-on-the-transciliary-supraorbital-keyhole-approach
#9
REVIEW
Daniel Walter Zumofen, Jonathan Rychen, Michel Roethlisberger, Ethan Taub, Daniel Kalbermatten, Erez Nossek, Matthew Potts, Raphael Guzman, Howard Antony Riina, Luigi Mariani
BACKGROUND: Conventional craniotomy approaches involve substantial soft tissue manipulation that can cause complications. The transciliary supraorbital keyhole approach was developed to avoid these complications. OBJECTIVE: To review the safety and effectiveness of the transciliary supraorbital keyhole approach. MATERIAL AND METHODS: We searched the PubMed/Medline database for full-text publications from 1996 onward containing data on 100 or more cases of aneurysm clipping or tumor resection by the transciliary supraorbital approach...
October 27, 2016: World Neurosurgery
https://www.readbyqxmd.com/read/27989902/alternate-update-of-shifted-extended-keyholes-ausek-a-new-accelerating-strategy-for-interventional-mri
#10
Shuai Yu, Derek Kwok, Yufu Zhou, Qing Zhang, Xiaoyan Huang, Geng Li, Bensheng Qiu
Real-time or near-real-time acquisition plays a key role in providing immediate image guidance for interventional magnetic resonance imaging (iMRI). However, the requirement of accurate needle tip localization has made several accelerating techniques, like Keyhole imaging or sliding window reconstruction, difficult to be applied to iMRI. The purpose of this work was to further explore the possible ways of applying view sharing techniques to iMRI. Inspired by Keyhole imaging, we present an easy-to-implement accelerating strategy called "Alternate update of shifted extended keyholes (AUSEK)"...
October 27, 2016: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/27981588/a-highly-specific-competitive-direct-enzyme-immunoassay-for-sterigmatocystin-as-a-tool-for-rapid-immunochemotaxonomic-differentiation-of-mycotoxigenic-aspergillus-species
#11
Sandra Wegner, Julia I Bauer, Richard Dietrich, Erwin Märtlbauer, Ewald Usleber, Christoph Gottschalk, Madeleine Gross
A simplified method to produce specific polyclonal rabbit antibodies against sterigmatocystin (STC) was established, using a STC-glycolic acid-ether derivative (STC-GE) conjugated to keyhole limpet hemocyanin (immunogen). The competitive direct enzyme immunoassay (EIA) established for STC had a detection limit (20% binding inhibition) of 130 pg ml(-1) . The test was highly specific for STC, with minor cross-reactivity with O-methylsterigmatocystin (OMSTC, 0.87%) and negligible reactivity with the aflatoxins (<0...
December 16, 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27956054/active-vaccination-attenuates-the-psychostimulant-effects-of-%C3%AE-pvp-and-mdpv-in-rats
#12
Jacques D Nguyen, Paul T Bremer, Alex Ducime, Kevin M Creehan, Brent R Kisby, Michael A Taffe, Kim D Janda
Recreational use of substituted cathinones continues to be an emerging public health problem in the United States; cathinone derivatives α-pyrrolidinopentiophenone (α-PVP) and 3,4-methylenedioxypyrovalerone (MDPV), which have been linked to human fatalities and show high potential for abuse liability in animal models, are of particular concern. The objective of this study was to develop an immunotherapeutic strategy for attenuating the effects of α-PVP and MDPV in rats, using drug-conjugate vaccines created to generate antibodies with neutralizing capacity...
December 9, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27938681/characterization-of-the-role-in-adherence-of-mycoplasma-hyorhinis-variable-lipoproteins-containing-different-repeat-unit-copy-numbers
#13
Qiyan Xiong, Bixiong Zhang, Jia Wang, Bo Ni, Yan Ji, Yanna Wei, Shaobo Xiao, Zhixin Feng, Maojun Liu, Guoqing Shao
Mycoplasma hyorhinis (M. hyorhinis) is an important pathogen of pigs. In previous studies, the variable lipoprotein (Vlp) family has been shown to play a role in mediating M. hyorhinis cytoadhesion. Herein, we performed several experiments to study the function of each Vlp family member in detail, especially examining the cytoadhesion functional domain and how the repeat unit copy number impacts on function. Recombinant proteins rVlpII, composed of region II from all seven Vlp members; rVlpIII, composed of repeat peptides from region III of all of Vlp members; as well as a series of recombinant rVlp proteins for each member containing different repeat unit copy numbers were constructed...
December 25, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27936222/combined-oral-and-intravenous-immunization-stimulates-strong-iga-responses-in-both-systemic-and-mucosal-compartments
#14
Zhe Yang, Qing Zhao, Yun-An Gao, Wei Zhang
To investigate the influence of immunization routes onIgG, IgA and IgM production in systemic and mucosal compartments, we immunized mice with keyhole limpet hemocyanin (KLH) via oral, intranasal (i.n.) or subcutaneous (s.c.) routes alone or combined with the intravenous (i.v.) route. We found that administering antigen intravenously could affect antibody production and formation of antibody secreting cells (ASCs) depending on the immunization route previously used. Combined oral/i.v. immunization but not s...
2016: PloS One
https://www.readbyqxmd.com/read/27898965/performance-of-pigs-kept-under-different-sanitary-conditions-affected-by-protein-intake-and-amino-acid-supplementation
#15
Y van der Meer, A Lammers, A J M Jansman, M M J A Rijnen, W H Hendriks, W J J Gerrits
There is growing evidence that requirements for particular AA increase when pigs are kept under low sanitary conditions. The extent to which reduction in growth performance is related to these increased requirements is unclear. To evaluate this relationship, an experiment (2 × 2 × 2 factorial arrangement) was performed with 612 male pigs (9 per pen) kept under low sanitary conditions (LSC) or high sanitary conditions (HSC) and offered ad libitum access to either a normal CP concentration diet (NP; 17, 15, and 15% CP for the starter, grower, and finisher phase, respectively) or a low CP concentration diet (LP; 20% CP reduced relative to NP for each phase), each of which containing a basal AA profile (AA-B) or a supplemented AA profile (AA-S)...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27873293/supraorbital-keyhole-microsurgical-fenestration-of-symptomatic-temporal-arachnoid-cysts-in-children-advantages-and-limitations
#16
Sherif Elkheshin, Ahmed Soliman
AIM: To investigate the impact of endoscope assisted microsurgical fenestration of temporal arachnoid cysts, the advantages and limitations of the technique. MATERIAL AND METHODS: Retrospective study including twenty five children of symptomatic temporal arachnoid cysts operated via Eyebrow Supra-Orbital Keyhole Microsurgical Fenestration targeting medial cyst wall, Preoperative MRI brain was done for all patients. RESULTS: Preoperative, clinical presentation included headache (80%), nausea & vomiting (64%), drug resistant epilepsy (52%), macrocephaly (12%) papilledema (28%) motor weakness in the form of right sided hemiparesis (12%) and cranial nerve palsy...
October 14, 2016: Turkish Neurosurgery
https://www.readbyqxmd.com/read/27866824/detection-of-her2-overexpressing-cancer-cells-using-keyhole-shaped-chamber-array-employing-a-magnetic-droplet-handling-system
#17
Mina Okochi, Shinji Koike, Masayoshi Tanaka, Hiroyuki Honda
An on-chip gene expression analysis compartmentalized in droplets was developed for detection of cancer cells at a single-cell level. The chip consists of a keyhole-shaped reaction chamber with hydrophobic modification employing a magnetic bead-droplet-handling system with a gate for bead separation. Using three kinds of water-based droplets in oil, a droplet with sample cells, a lysis buffer with magnetic beads, and RT-PCR buffer, parallel magnetic manipulation and fusion of droplets were performed using a magnet-handling device containing small external magnet patterns in an array...
November 11, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27866381/cardiac-magnetic-resonance-imaging-of-patent-ductus-arteriosus-in-three-dogs
#18
Youngjae Lee, Jaihyun Jung, Junyong Park, Jiyoon Jeong, Seokho Jeon, Sunyoung Park, Jinhwa Chang, Ji-Houn Kang, Chulhyun Lee, Dongwoo Chang
Patent ductus arteriosus (PDA) is the most common congenital cardiovascular disorder in dogs and requires an accurate diagnosis for an appropriate treatment. Cardiac MRI (cMRI) has been reported as a method for characterization of canine thoracic vasculature. However, to the authors' knowledge, no published studies describe evaluation of canine PDA through cMRI. Three dogs were selected for this exploratory study. Electrocardiogram gating and breath-hold techniques were performed using a 3T MR scanner. Both black blood imaging and bright blood cine acquisitions were performed...
November 20, 2016: Veterinary Radiology & Ultrasound
https://www.readbyqxmd.com/read/27849166/first-in-human-study-in-healthy-subjects-with-fr104-a-pegylated-monoclonal-antibody-fragment-antagonist-of-cd28
#19
Nicolas Poirier, Gilles Blancho, Maryvonne Hiance, Caroline Mary, Tim Van Assche, Jos Lempoels, Steven Ramael, Weirong Wang, Virginie Thepenier, Cecile Braudeau, Nina Salabert, Regis Josien, Ian Anderson, Ian Gourley, Jean-Paul Soulillou, Didier Coquoz, Bernard Vanhove
FR104 is a monovalent pegylated Fab' Ab, antagonist of CD28, under development for treatment of transplant rejection and autoimmune diseases. In contrast to CD80/86 antagonists (CTLA4-Ig), FR104 selectively blunts CD28 costimulation while sparing CTLA-4 and PD-L1 coinhibitory signals. In the present work, FR104 has been evaluated in a first-in-human study to evaluate the safety, pharmacokinetics, pharmacodynamics, and potency of i.v. administrations in healthy subjects. Sixty-four subjects were randomly assigned to four single ascending dose groups, two double dose groups and four single ascending dose groups challenged with keyhole limpet hemocyanin...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27847568/intradural-procedural-time-to-assess-technical-difficulty-of-superciliary-keyhole-and-pterional-approaches-for-unruptured-middle-cerebral-artery-aneurysms
#20
Yeon-Ju Choi, Wonsoo Son, Ki-Su Park, Jaechan Park
OBJECTIVE: This study used the intradural procedural time to assess the overall technical difficulty involved in surgically clipping an unruptured middle cerebral artery (MCA) aneurysm via a pterional or superciliary approach. The clinical and radiological variables affecting the intradural procedural time were investigated, and the intradural procedural time compared between a superciliary keyhole approach and a pterional approach. METHODS: During a 5.5-year period, patients with a single MCA aneurysm were enrolled in this retrospective study...
November 2016: Journal of Korean Neurosurgical Society
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