Kui Jin, Yujie Ma, Carlos L Manrique-Caballero, Hui Li, David R Emlet, Shengnan Li, Catherine J Baty, Xiaoyan Wen, Nahmah Kim-Campbell, Alicia Frank, Elizabeth V Menchikova, Nuria M Pastor-Soler, Kenneth R Hallows, Edwin K Jackson, Sruti Shiva, Michael R Pinsky, Brian S Zuckerbraun, John A Kellum, Hernando Gómez
The purpose was to determine the role of AMPK activation in the renal metabolic response to sepsis, the development of sepsis-induced acute kidney injury (AKI) and on survival. In a prospective experimental study, 167 10- to 12-week-old C57BL/6 mice underwent cecal ligation and puncture (CLP) and human proximal tubule epithelial cells (TEC; HK2) were exposed to inflammatory mix (IM), a combination of lipopolysaccharide (LPS) and high mobility group box 1 (HMGB1). Renal/TEC metabolic fitness was assessed by monitoring the expression of drivers of oxidative phosphorylation (OXPHOS), the rates of utilization of OXPHOS/glycolysis in response to metabolic stress, and mitochondrial function by measuring O2 consumption rates (OCR) and the membrane potential (Δψm )...
May 2020: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology