Ying Wang, Yu-Xiang Han, Zhi-Yun Niu, Xing-Zhe Wang, Huan Hua, Yin-Tao Shang, Fu-Xu Wang, Xue-Jun Zhang, Jian-Min Luo
The purpose of this study was to investigate the effect of bone marrow mesenchymal stem cells (BMMSC) from patients with chronic myeloid leukemia (CML) in blastic phase (Bp) on K562 cells and the primary CML-Bp cells, and to explore its potential mechanisms. K562 cells and primary CML-Bp cells were co-cultured with BMMSC of different groups; the cell proliferation was detected by MTT method, the cell apoptosis rate and mitochondrial membrane potential were measured by flow cytometry, the expression levels of Caspase-8, Caspase-9, and activated Caspase-3 in cells were measured by Western blot...
October 2014: Zhongguo Shi Yan Xue Ye Xue za Zhi