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Hui Wang, Si-Hong Liu, Yuan-Mei Zhai, Xiao-Yu Zhu, Yun Wan, Wei Lu, Jun Shi
OBJECTIVE: To investigate the effect of metformin on 3T3-L1 preadipocyte's differentiation and consequently observe the anti-proliferative effects of metformin-treated adipocytes on leukemia cells. METHODS: Different concentrations of metformin were added in 3T3-L1 preadipocytes to induce maturation, the matured adipocytes were detected by oil red O staining and quantified by absorbance value (OD). Real-time PCR was used to detect the mRNA expression level of the key adipogenic genes PPARγ, C/EBPα and FABP4(ap2)...
April 2015: Zhongguo Shi Yan Xue Ye Xue za Zhi
Ying Wang, Yu-Xiang Han, Zhi-Yun Niu, Xing-Zhe Wang, Huan Hua, Yin-Tao Shang, Fu-Xu Wang, Xue-Jun Zhang, Jian-Min Luo
The purpose of this study was to investigate the effect of bone marrow mesenchymal stem cells (BMMSC) from patients with chronic myeloid leukemia (CML) in blastic phase (Bp) on K562 cells and the primary CML-Bp cells, and to explore its potential mechanisms. K562 cells and primary CML-Bp cells were co-cultured with BMMSC of different groups; the cell proliferation was detected by MTT method, the cell apoptosis rate and mitochondrial membrane potential were measured by flow cytometry, the expression levels of Caspase-8, Caspase-9, and activated Caspase-3 in cells were measured by Western blot...
October 2014: Zhongguo Shi Yan Xue Ye Xue za Zhi
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