keyword
MENU ▼
Read by QxMD icon Read
search

bioorthogonal click

keyword
https://www.readbyqxmd.com/read/29443107/visualizing-lignification-dynamics-in-plants-with-click-chemistry-dual-labeling-is-bliss
#1
Clémence Simon, Corentin Spriet, Simon Hawkins, Cedric Lion
Lignin is one of the most prevalent biopolymers on the planet and a major component of lignocellulosic biomass. This phenolic polymer plays a vital structural and protective role in the development and life of higher plants. Although the intricate mechanisms regulating lignification processes in vivo strongly impact the industrial valorization of many plant-derived products, the scientific community still has a long way to go to decipher them. In a simple three-step workflow, the dual labeling protocol presented herein enables bioimaging studies of actively lignifying zones of plant tissues...
January 26, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29410228/facile-metabolic-glycan-labeling-strategy-for-exosome-tracking
#2
Tae Sup Lee, Young Kim, Weiqi Zhang, In Ho Song, Ching-Hsuan Tung
BACKGROUND: Exosomes are nano-sized vesicles derived from the fusion of multivesicular bodies with the surrounding plasma membrane. Exosomes have various diagnostic and therapeutic potentials in cancer and other diseases, thus tracking exosomes is an important issue. METHODS: Here, we report a facile exosome labeling strategy using a natural metabolic incorporation of an azido-sugar into the glycan, and a strain-promoted azide-alkyne click reaction. In culture, tetra-acetylated N-azidoacetyl-D-mannosamine (Ac4ManNAz) was spontaneously incorporated into glycans within the cells and later redistributed onto their exosomes...
February 2, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29394044/thermal-stabilization-of-biologics-with-photoresponsive-hydrogels
#3
Balaji V Sridhar, John R Janczy, Øyvind Hatlevik, Gabriel Wolfson, Kristi S Anseth, Mark W Tibbitt
Modern medicine, biological research, and clinical diagnostics depend on the reli- able supply and storage of complex biomolecules. However, biomolecules are inherently susceptible to thermal stress and the global distribution of value-added biologics, including vaccines, biotherapeutics, and Research Use Only (RUO) proteins, requires an integrated cold chain from point of manufacture to point of use. To mitigate reliance on the cold chain, formulations have been engineered to protect biologics from thermal stress, including materials-based strategies that impart thermal stability via direct encapsulation of the molecule...
February 2, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29388773/sydnone-reporters-for-highly-fluorogenic-copper-free-click-ligations
#4
Camille Favre, Lucie de Cremoux, Jerome Badaut, Frédéric Friscourt
Bioorthogonal fluorescent turn-on reactions are attractive for the sensitive real-time detection of a variety of phenomena including bioconjugation, chemical reactivity, and material assembly. Herein we describe the use of 3,4-disubstituted sydnones, a singular class of mesoionic dipoles, for highly fluorescent turn-on copper-free click cycloadditions with the fluorogenic dibenzocyclooctyne Fl-DIBO. Coherent with time-dependent density functional theory calculations, the pyrazole cycloadducts were found to be highly fluorescent with compelling photophysical properties including excellent fluorescence enhancement (up to 240-fold), high quantum yields (over 45%), and large Stokes shift (over 100 nm)...
February 1, 2018: Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29384666/unraveling-tetrazine-triggered-bioorthogonal-elimination-enables-chemical-tools-for-ultrafast-release-and-universal-cleavage
#5
Jonathan Carlson, Hannes Mikula, Ralph Weissleder
Recent developments in bond cleavage reactions have expanded the scope of bioorthogonal chemistry beyond click ligation and enabled new strategies for probe activation and therapeutic delivery. These applications, however, remain in their infancy, with further innovations needed to achieve the efficiency required for versatile and broadly useful tools in vivo. Amongst these chemistries, the tetrazine/trans-cyclooctene click-to-release reaction has exemplary kinetics and adaptability but achieves only partial release and is incompletely understood, which has limited its application...
January 31, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29356431/precision-fluorescent-labeling-of-an-adeno-associated-virus-vector-to-monitor-the-viral-infection-pathway
#6
Chuanling Zhang, Xueying Zhou, Tianzhuo Yao, Zhenyu Tian, Demin Zhou
Adeno-associated virus 2 (AAV2) is a common vehicle for the delivery of a variety of therapeutic genes. A better understanding of the process of infection of AAV2 will advance our knowledge of AAV2 biology and allow for the optimization of AAV2 capsids with favorable transduction profiles. However, the precise fluorescent labeling of an AAV2 vector for probing virus tracking without affecting the nature of the virus remains a challenge. In this study, we precisely displayed lab-synthesized azide-moieties on the viral capsid at modifiable sites...
January 22, 2018: Biotechnology Journal
https://www.readbyqxmd.com/read/29306547/a-cell-surface-clicked-navigation-system-to-direct-specific-bone-targeting
#7
Young Kim, Zhe Zhang, Jae-Hyuck Shim, Tae Sup Lee, Ching-Hsuan Tung
Cell therapies are promising up-and-coming therapeutic strategies for many diseases. For maximal therapeutic benefits, injected cells have to navigate their way to a designated area, including organ and tissue; unfortunately, the majority of therapeutic cells are currently administered without a guide or homing device. To improve this serious shortcoming, a functionalization method was developed to equip cells with a homing signal. Its application was demonstrated by applying an Azadibenzocyclooctyne-bisphosphonate (ADIBO-BP) and azide paired bioorthogonal chemistry on cells for bone specific homing...
December 26, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29250049/using-click-chemistry-for-visualizing-in-situ-changes-of-translational-activity-in-planktonic-marine-bacteria
#8
Ainara Leizeaga, Margarita Estrany, Irene Forn, Marta Sebastián
A major challenge in microbial ecology is linking diversity and function to determine which microbes are actively contributing to processes occurring in situ. Bioorthogonal non-canonical amino acid tagging (BONCAT) is a promising technique for detecting and quantifying translationally active bacteria in the environment. This technique consists of incubating a bacterial sample with an analog of methionine and using click-chemistry to identify the cells that have incorporated the substrate. Here, we established an optimized protocol for the visualization of protein-synthesizing cells in oligotrophic waters that can be coupled with taxonomic identification using Catalyzed Reporter Deposition Fluorescent in Situ Hybridization...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29240290/bioorthogonal-cycloadditions-with-sub-millisecond-intermediates
#9
Yujia Qing, Gökçe Su Pulcu, Nicolas Bell, Hagan Bayley
Tetrazine- and sydnone-based click chemistries have emerged as important bioconjugation strategies with fast kinetics and N2 or CO2 as the only by-product. Mechanistic studies of these reactions have focused on the initial rate-determining cycloaddition steps. The subsequent N2 or CO2 release from the bicyclic intermediates has been approached mainly through computational studies, which have predicted lifetimes of femtoseconds. In the present study, bioorthogonal cycloadditions involving N2 or CO2 extrusion have been examined experimentally at the single-molecule level by using a protein nanoreactor...
December 14, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29192289/caspase-3-7-specific-metabolic-precursor-for-bioorthogonal-tracking-of-tumor-apoptosis
#10
Man Kyu Shim, Hong Yeol Yoon, Sangmin Lee, Mun Kyeong Jo, Jooho Park, Jong-Ho Kim, Seo Young Jeong, Ick Chan Kwon, Kwangmeyung Kim
Apoptosis is one of the most important intracellular events in living cell, which is a programmed cell death interrelated with caspase enzyme activity for maintaining homeostasis in multicellular organisms. Therefore, direct apoptosis imaging of living cells can provide enormous advantages for diagnosis, drug discovery, and therapeutic monitoring in various diseases. However, a method of direct apoptosis imaging has not been fully validated, especially for live cells in in vitro and in vivo. Herein, we developed a new apoptosis imaging technology via a direct visualization of active caspase-3/-7 activity in living cells...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29130210/imaging-newly-transcribed-rna-in-cells-by-using-a-clickable-azide-modified-utp-analog
#11
Anupam A Sawant, Sanjeev Galande, Seergazhi G Srivatsan
Robust RNA labeling and imaging methods that enable the understanding of cellular RNA biogenesis and function are highly desired. In this context, we describe a practical chemical labeling method based on a bioorthogonal reaction, namely, azide-alkyne cycloaddition reaction, which facilitates the fluorescence imaging of newly transcribed RNA in both fixed and live cells. This strategy involves the transfection of an azide-modified UTP analog (AMUTP) into mammalian cells, which gets specifically incorporated into RNA transcripts by RNA polymerases present inside the cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29109777/improved-tumor-targeting-and-longer-retention-time-of-nir-fluorescent-probes-using-bioorthogonal-chemistry
#12
Xianghan Zhang, Bo Wang, Na Zhao, Zuhong Tian, Yunpeng Dai, Yongzhan Nie, Jie Tian, Zhongliang Wang, Xiaoyuan Chen
The traditional labeling method for targeted NIR fluorescence probes requires directly covalent-bonded conjugation of targeting domains and fluorophores in vitro. Although this strategy works well, it is not sufficient for detecting or treating cancers in vivo, due to steric hindrance effects that relatively large fluorophore molecules exert on the configurations and physiological functions of specific targeting domains. The copper-free, "click-chemistry"-assisted assembly of small molecules in living systems may enhance tumor accumulation of fluorescence probes by improving the binding affinities of the targeting factors...
2017: Theranostics
https://www.readbyqxmd.com/read/29095606/ex-uno-plura-differential-labeling-of-phospholipid-biosynthetic-pathways-with-a-single-bioorthogonal-alcohol
#13
Timothy W Bumpus, Felice J Liang, Jeremy M Baskin
Imaging approaches that track biological molecules within cells are essential tools in modern biochemistry. Lipids are particularly challenging to visualize, as they are not directly genetically encoded. Phospholipids, the most abundant subgroup of lipids, are structurally diverse and accomplish many cellular functions, acting as major structural components of membranes and as signaling molecules that regulate cell growth, division, apoptosis, cytoskeletal dynamics, and numerous other physiological processes...
November 8, 2017: Biochemistry
https://www.readbyqxmd.com/read/29048718/azido-functionalized-5-cap-analogs-for-preparation-of-translationally-active-mrnas-suitable-for-fluorescent-labeling-in-living-cells
#14
Adam Mamot, Pawel Sikorski, Marcin Warminski, Joanna Kowalska, Jacek Jemielity
The 7-methylguanosine (m7G) cap structure is a unique feature present at the 5' ends of messenger RNAs (mRNAs) that can be subjected to extensive modifications, resulting in alterations to mRNA properties (e.g. translability, susceptibility to degradation). It also can provide molecular tools to study mRNA metabolism. Here, we developed new mRNA 5' cap-based tools that enable the site-specific labeling of RNA at the 5' end using strain-promoted azide-alkyne cycloaddition (SPAAC), i.e. bioorthogonal, copper-free click chemistry, and support the mRNA's basic function in protein biosynthesis...
October 19, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29044843/discovery-of-new-click-and-release-reactions-by-screening-of-mesoionics-and-cycloalkynes-combinations
#15
Frédéric Taran, Sabrina Bernard, Davide Audisio, Riomet Margaux, Sarah Bregant, Antoine Sallustrau, Lucie Plougastel, Elodie Decuypere, Sandra Gabillet, Ramar Arun Kumar, Jijy Elyian, Minh Nguyet Trinh, Oleksandr Koniev, Alain Wagner, Sergii Kolodych
We report the discovery of a new bioorthogonal click and release reaction involving imino-sydnones and strained alkynes. This transformation leads to two products resulting from both ligation and fragmentation of imino-sydnones under physiological conditions. Optimized imino-sydnones were successfully used to design innovative cleavable linkers for protein modifications opening new areas in the fields of drug release and target fishing applications. This click and release technology offers for the first time the possibility to exchange tags on proteins with functionalized cyclooctynes under mild and bioorthogonal conditions...
October 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29035405/visible-light-mediated-cleavage-of-polymer-chains-under-physiological-conditions-via-quinone-photoreduction-and-trimethyl-lock
#16
Vinh X Truong, Fanyi Li, Francesca Ercole, John S Forsythe
We introduce a click and visible-light triggered unclick approach via thio-bromo reaction and hydroquinone photoreduction/trimethyl lock cleavage for polymer modifications. Both reactions can be carried out in water and at ambient temperature, enabling preparation of bioorthogonal hydrogels for encapsulation and controlled release of various cells.
November 7, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28985740/nanoscale-click-reactive-scaffolds-from-peptide-self-assembly
#17
Alexander P M Guttenplan, Laurence J Young, Dijana Matak-Vinkovic, Clemens F Kaminski, Tuomas P J Knowles, Laura S Itzhaki
BACKGROUND: Due to their natural tendency to self-assemble, proteins and peptides are important components for organic nanotechnology. One particular class of peptides of recent interest is those that form amyloid fibrils, as this self-assembly results in extremely strong, stable quasi-one-dimensional structures which can be used to organise a wide range of cargo species including proteins and oligonucleotides. However, assembly of peptides already conjugated to proteins is limited to cargo species that do not interfere sterically with the assembly process or misfold under the harsh conditions often used for assembly...
October 6, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28971673/integrating-two-efficient-and-specific-bioorthogonal-ligation-reactions-with-natural-metabolic-incorporation-in-one-cell-for-virus-dual-labeling
#18
Li-Li Huang, Kejiang Liu, Qianmei Zhang, Jin Xu, Dongxu Zhao, Houshun Zhu, Hai-Yan Xie
Though techniques in bioorthogonal chemistry and metabolic incorporation have been developed over the past decade, it remains difficult to integrate different bioorthogonal reactions or metabolic incorporations into one system. In this report, the protein and DNA metabolic incorporations were combined with two bioorthogonal reactions in one cell to develop a facile and universal method for virus dual labeling. Azide and vinyl groups were introduced into the proteins or genomes of viruses, respectively, through the intrinsic biosynthesis of biomolecules, which were subsequently fluorescently labeled via copper-free click chemistry or alkene-tetrazine ligation reactions during natural propagation process in host cells...
November 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28957709/nano-sized-metabolic-precursors-for-heterogeneous-tumor-targeting-strategy-using-bioorthogonal-click-chemistry-in%C3%A2-vivo
#19
Sangmin Lee, Seulhee Jung, Heebeom Koo, Jin Hee Na, Hong Yeol Yoon, Man Kyu Shim, Jooho Park, Jong-Ho Kim, Seulki Lee, Martin G Pomper, Ick Chan Kwon, Cheol-Hee Ahn, Kwangmeyung Kim
Herein, we developed nano-sized metabolic precursors (Nano-MPs) for new tumor-targeting strategy to overcome the intrinsic limitations of biological ligands such as the limited number of biological receptors and the heterogeneity in tumor tissues. We conjugated the azide group-containing metabolic precursors, triacetylated N-azidoacetyl-d-mannosamine to generation 4 poly(amidoamine) dendrimer backbone. The nano-sized dendrimer of Nano-MPs could generate azide groups on the surface of tumor cells homogeneously regardless of cell types via metabolic glycoengineering...
December 2017: Biomaterials
https://www.readbyqxmd.com/read/28926550/bioorthogonal-chemistry-click-on-click-off
#20
Caitlin Deane
No abstract text is available yet for this article.
September 19, 2017: Nature Chemical Biology
keyword
keyword
91141
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"