keyword
https://read.qxmd.com/read/38484471/chemical-probes-to-interrogate-the-extreme-environment-of-mosquito-larval-guts
#1
JOURNAL ARTICLE
Lindsay E Guzmán, Anjalee N Wijetunge, Brendan F Riske, Brooke B Massani, Michael A Riehle, John C Jewett
Mosquito control methods are vital to curtail the spread of life-threatening illnesses, such as dengue fever, malaria, and yellow fever. Vector control technologies must be selective to minimize deleterious effects on our ecosystem. Successful methods that control mosquito larva populations utilize the uniquely high alkaline nature of the midgut. Here, we present novel protected triazabutadienes (pTBD) that are deprotected under basic conditions of the larval midgut, releasing an aryl diazonium ion (ADI) that results in protein modification...
March 14, 2024: Journal of the American Chemical Society
https://read.qxmd.com/read/38482815/bioorthogonal-radiolabeling-of-azide-modified-bacteria-using-18-f-fb-sulfo-dbco
#2
JOURNAL ARTICLE
Aryn A Alanizi, Alexandre M Sorlin, Matthew F L Parker, Marina López-Álvarez, Hecong Qin, Sang Hee Lee, Joseph Blecha, Oren S Rosenberg, Joanne Engel, Michael A Ohliger, Robert R Flavell, David M Wilson
Purpose: This study was motivated by the need for better positron emission tomography (PET)-compatible tools to image bacterial infection. Our previous efforts have targeted bacteria-specific metabolism via assimilation of carbon-11 labeled d-amino acids into the bacterial cell wall. Since the chemical determinants of this incorporation are not fully understood, we sought a high-throughput method to label d-amino acid derived structures with fluorine-18. Our strategy employed a chemical biology approach, whereby an azide (-N3 ) bearing d-amino acid is incorporated into peptidoglycan muropeptides, with subsequent "click" cycloaddition with an 18 F-labeled strained cyclooctyne partner...
March 14, 2024: Bioconjugate Chemistry
https://read.qxmd.com/read/38470564/injectable-and-multifunctional-hydrogels-based-on-poly-n-acryloyl-glycinamide-and-alginate-derivatives-for-antitumor-drug-delivery
#3
JOURNAL ARTICLE
Trung Thang Vu, Sung-Han Jo, Seon-Hwa Kim, Byeong Kook Kim, Sang-Hyug Park, Kwon Taek Lim
Chemotherapy is a conventional treatment that uses drugs to kill cancer cells; however, it may induce side effects and may be incompletely effective, leading to the risk of tumor recurrence. To address this issue, we developed novel injectable thermal/near-infrared (NIR)-responsive hydrogels to control drug release. The injectable hydrogel formulation was composed of biocompatible alginates, poly( N -acryloyl glycinamide) (PNAGA) copolymers with an upper critical solution temperature, and NIR-responsive cross-linkers containing coumarin groups, which were gelated through bioorthogonal inverse electron demand Diels-Alder reactions...
March 12, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38464126/pet-mr-guided-pre-targeted-delivery-to-her2-breast-cancer-model
#4
Ge Si, Sudath Hapuarachchige, Wojciech Lesniak, Dmitri Artemov
Purpose: HER2(+) metastatic breast cancer (mBC) is one of the most aggressive and lethal cancer types among females. While initially effective, targeted therapeutic approaches with trastuzumab and pertuzumab antibodies and antibody-drug conjugates (ADC) lack long-term efficacy against HER2(+) mBC and can cause severe systemic toxicity due to off-target effects. Therefore, the development of novel targeted delivery platforms that minimize toxicity and increase therapeutic efficacy is critical to the treatment of HER2(+) breast cancer (BC)...
February 28, 2024: Research Square
https://read.qxmd.com/read/38462598/a-proof-of-concept-study-on-bioorthogonal-based-pretargeting-and-signal-amplify-radiotheranostic-strategy
#5
JOURNAL ARTICLE
Hongzhang Yang, Xinying Zeng, Jia Liu, Jingchao Li, Yun Li, Qinglin Zhang, Linlin Shu, Huanhuan Liu, Xueqi Wang, Yuanyuan Liang, Ji Hu, Lumei Huang, Zhide Guo, Xianzhong Zhang
BACKGROUND: Radiotheranostics differs from the vast majority of other cancer therapies in its capacity for simultaneous imaging and therapy, and it is becoming more widely implemented. A balance between diagnostic and treatment requirements is essential for achieving effective radiotheranostics. Herein, we propose a proof-of-concept strategy aiming to address the profound differences in the specific requirements of the diagnosis and treatment of radiotheranostics. RESULTS: To validate the concept, we designed an s-tetrazine (Tz) conjugated prostate-specific membrane antigen (PSMA) ligand (DOTA-PSMA-Tz) for 68 Ga or 177 Lu radiolabeling and tumor radiotheranostics, a trans-cyclooctene (TCO) modified Pd@Au nanoplates (Pd@Au-PEG-TCO) for signal amplification, respectively...
March 10, 2024: Journal of Nanobiotechnology
https://read.qxmd.com/read/38456184/double-cross-linked-graphene-oxide-hydrogel-for-promoting-healing-of-diabetic-ulcers
#6
JOURNAL ARTICLE
Wenxu Liu, Yunfang Yang, Meiying Li, Jingxin Mo
This study explores the synthesis and characterization of a novel double cross-linked hydrogel composed of polyvinyl alcohol (PVA), sodium alginate (SA), graphene oxide (GO), and glutathione (GSH), henceforth referred to as PVA/SA/GO/GSH. This innovative hydrogel system incorporates two distinct types of cross-linking networks and is meticulously engineered to exhibit sensitivity to high glucose and/or reactive oxygen species (ROS) environments. A sequential approach was adopted in the hydrogel formation. The initial phase involved the absorption of GSH onto GO, which was subsequently functionalized with boric acid and polyethylene glycol derivatives via a bio-orthogonal click reaction...
2024: Frontiers in Chemistry
https://read.qxmd.com/read/38451232/synthesis-of-sydnonimines-from-sydnones-and-their-use-for-bioorthogonal-release-of-isocyanates-in-cells
#7
JOURNAL ARTICLE
Judith Baudet, Emilie Lesur, Maxime Ribéraud, Arnaud Chevalier, Timothée D'Anfray, Pierre Thuéry, Davide Audisio, Frédéric Taran
In this article, we report the synthesis of sydnonimines from sydnones and their use as dipoles for fast click-and-release reactions. The process relies on nucleophilic aromatic substitution of aliphatic and aromatic amines with triflated sydnones. This new methodology allowed the preparation of functionalised sydnonimine probes that are otherwise difficult to prepare. These probes were then used to release a drug and a fluorescent aromatic isocyanate inside living cells.
March 7, 2024: Chemical Communications: Chem Comm
https://read.qxmd.com/read/38401443/covalent-coupling-of-functionalized-outer-membrane-vesicles-omvs-to-gold-nanoparticles
#8
JOURNAL ARTICLE
Ji-Hong Bong, Alexander Dombovski, Robin Birus, Sua Cho, Misu Lee, Jae-Chul Pyun, Joachim Jose
Outer membrane vesicle-functionalized nanoparticles (OMV-NPs) have attracted significant interest, especially regarding drug delivery applications and vaccines. Here, we report on novel OMV-NPs by applying bioorthogonal click reaction for encapsulating gold nanoparticles (NPs) within outer membrane vesicles (OMVs) by covalent coupling. For this purpose, outer membrane protein A (OmpA), abundant in large numbers (due to 100,000 copies/cell [1]) in OMVs, was modified via the incorporation of the unnatural amino acid p-azidophenylalanine...
February 18, 2024: Journal of Colloid and Interface Science
https://read.qxmd.com/read/38331133/minimally-invasive-delivery-of-human-umbilical-cord-derived-mesenchymal-stem-cells-by-an-injectable-hydrogel-via-diels-alder-click-reaction-for-the-treatment-of-intrauterine-adhesions
#9
JOURNAL ARTICLE
Sentao Hu, Yangyang Dai, Liaobing Xin, Xiaowen Zheng, Zi Ye, Songying Zhang, Lie Ma
Intrauterine adhesions (IUA) are the most common cause of uterine infertility, and conventional treatments have not consistently achieved satisfactory pregnancy rates. Stem cell therapy shows promising potential for the clinical treatment of IUA. Although various advanced biomaterials have been designed for delivering stem cells to the uterine cavity, there remain significant challenges, particularly in devising therapeutic strategies for clinical application that minimize surgical incisions and conform to the intricate structure of uterine cavity...
February 6, 2024: Acta Biomaterialia
https://read.qxmd.com/read/38326078/live-organoid-cyclic-imaging
#10
JOURNAL ARTICLE
David E Reynolds, Yusha Sun, Xin Wang, Phoebe Vallapureddy, Jianhua Lim, Menghan Pan, Andres Fernandez Del Castillo, Jonathan C T Carlson, Mark A Sellmyer, MacLean Nasrallah, Zev Binder, Donald M O'Rourke, Guo-Li Ming, Hongjun Song, Jina Ko
Organoids are becoming increasingly relevant in biology and medicine for their physiological complexity and accuracy in modeling human disease. To fully assess their biological profile while preserving their spatial information, spatiotemporal imaging tools are warranted. While previously developed imaging techniques, such as four-dimensional (4D) live imaging and light-sheet imaging have yielded important clinical insights, these technologies lack the combination of cyclic and multiplexed analysis. To address these challenges, bioorthogonal click chemistry is applied to display the first demonstration of multiplexed cyclic imaging of live and fixed patient-derived glioblastoma tumor organoids...
February 7, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://read.qxmd.com/read/38313360/chemical-and-biological-engineering-strategies-to-make-and-modify-next-generation-hydrogel-biomaterials
#11
JOURNAL ARTICLE
Ryan Gharios, Ryan M Francis, Cole A DeForest
There is a growing interest in the development of technologies to probe and direct in vitro cellular function for fundamental organoid and stem cell biology, functional tissue and metabolic engineering, and biotherapeutic formulation. Recapitulating many critical aspects of the native cellular niche, hydrogel biomaterials have proven to be a defining platform technology in this space, catapulting biological investigation from traditional two-dimensional (2D) culture into the 3D world. Seeking to better emulate the dynamic heterogeneity characteristic of all living tissues, global efforts over the last several years have centered around upgrading hydrogel design from relatively simple and static architectures into stimuli-responsive and spatiotemporally evolvable niches...
December 6, 2023: Matter
https://read.qxmd.com/read/38303647/bioorthogonal-click-and-release-reaction-triggered-aggregation-of-gold-nanoparticles-combined-with-released-lonidamine-for-enhanced-cancer-photothermal-therapy
#12
JOURNAL ARTICLE
Xiao Yan, Ke Li, Tian-Qiu Xie, Xiao-Kang Jin, Cheng Zhang, Qian-Ru Li, Jun Feng, Chuan-Jun Liu, Xian-Zheng Zhang
Cancer has been the most deadly disease, and 13 million cancer casualties are estimated to occur each year by 2030. Gold nanoparticles (AuNPs)-based photothermal therapy (PTT) has attracted great interest due to its high spatiotemporal controllability and noninvasiveness. Due to the trade-off between particle size and photothermal efficiency of AuNPs, rational design is needed to realize aggregation of AuNPs into larger particles with desirable NIR adsorption in tumor site. Exploiting the bioorthogonal "Click and Release" (BCR) reaction between iminosydnone and cycloalkyne, aggregation of AuNPs can be achieved and attractively accompanied by the release of chemotherapeutic drug purposed to photothermal synergizing...
February 2, 2024: Angewandte Chemie
https://read.qxmd.com/read/38294888/engineered-metallic-ion-based-hydrogel-for-tendon-bone-reconstruction
#13
JOURNAL ARTICLE
Xinyu Zhang, Jun Ma, Hanyin Hu, Zhuoming Xu, Jintao Liu, Jiayi Chen, Bin Chen, Lili Shi, Huanhuan Luo, Gang Chen, Hongwei Xu
Rotator cuff regeneration is hindered by compromised vascular architecture, inflammation, and instability of the reconstructed tendon-bone interface. Herein, inspired by the phenomenon of magnetic clasps being connected together by a specific structure, an engineered metallic ion-based hydrogel scaffold was constructed through a bioorthogonal click reaction between (DOPA)4 -PEG5 -N3 and DBCO-BMP-2 peptides and a photopolymerization process in the hydrogel matrix, exhibiting the potential for angiogenesis, bone regeneration, and modulation of the inflammatory milieu, which aimed at facilitating rotator cuff regeneration...
January 31, 2024: ACS Applied Materials & Interfaces
https://read.qxmd.com/read/38270077/durable-attenuation-of-tumor-ph-platelet-linkage-reinstates-bioorthogonal-targeting-of-residual-tumors-post-debulking
#14
JOURNAL ARTICLE
Ping Zhang, Bo Li, Ziyan Wang, Junlin Li, Fengju Wang, Jinxia Kong, Zhou Zhou, Yuan Huang, Lian Li
There are circumstances where tumors can only be partially resected. Therefore, multimodality therapy targeting post-operative residuals is important. Here, we show that bioorthogonal click chemistry enables targeted delivery to heterogeneous tumors, but its utility against tumor post-debulking is ineffective due to platelet cloaks that shield tumor cells from bioorthogonal pairing. We further discover tumor-infiltrating platelet levels respond to local pH changes. Elucidating this pH-platelet linkage, we design an injectable hydrogel for resection cavity implantation that simultaneously azido-tags tumor cells and inhibit their catalysis to acidify surrounding milieu...
January 25, 2024: ACS Nano
https://read.qxmd.com/read/38234773/chemical-probes-to-interrogate-the-extreme-environment-of-mosquito-larval-guts
#15
Lindsay E Guzmán, Anjalee N Wijetunge, Brendan F Riske, Brooke B Massani, Michael A Riehle, John C Jewett
Mosquito control methods are vital for the spread of life-threatening illnesses such as dengue fever, malaria, and yellow fever. Vector control technologies must be selective to minimize deleterious effects to our ecosystem. Successful methods that control mosquito larva populations utilize the uniquely high alkaline nature of the midgut. Here, we present novel protected triazabutadienes (pTBD) which are deprotected under basic conditions of the larval midgut, releasing an aryl diazonium ion (ADI) that results in protein modification...
December 27, 2023: bioRxiv
https://read.qxmd.com/read/38233359/senolysis-enabled-by-senescent-cell-sensitive-bioorthogonal%C3%A2-tetrazine-ligation
#16
JOURNAL ARTICLE
Mengyang Chang, Yue Dong, Hang Xu, Alexis B Cruickshank-Taylor, Jacob S Kozora, Baran Behpour, Wei Wang
Although clearance of senescent cells has been proved to slow down aging process and promote anti-cancer chemotherapy, the development of senolytics remains challenging. Herein, we report a senolytic strategy enabled by senescent cell-sensitive bioorthogonal tetrazine ligation. Our design is engineering a galactose (Gal) moiety into dihydrotetrazine, which serves both as recognition moiety for senescence-associated β-galactosidase (SA-β-gal) and a caging group for control of tetrazine activity. We demonstrate that Gal-Tz enables efficient click-release of fluorescent HCA and doxorubicin (Dox) from TCO caged HCA and Dox prodrug to detect and eliminate senescent HeLa and A549 cells over non-senescent counterparts with 16...
January 17, 2024: Angewandte Chemie
https://read.qxmd.com/read/38232732/bioorthogonal-click-labeling-of-an-amber-free-hiv-1-provirus-for-in-virus-single-molecule-imaging
#17
JOURNAL ARTICLE
Yuanyun Ao, Jonathan R Grover, Levi Gifford, Yang Han, Guohua Zhong, Revansiddha Katte, Wenwei Li, Rajanya Bhattacharjee, Baoshan Zhang, Stephanie Sauve, Wenyi Qin, Dibya Ghimire, Md Anzarul Haque, James Arthos, Mahmoud Moradi, Walther Mothes, Edward A Lemke, Peter D Kwong, Gregory B Melikyan, Maolin Lu
Structural dynamics of human immunodeficiency virus 1 (HIV-1) envelope (Env) glycoprotein mediate cell entry and facilitate immune evasion. Single-molecule FRET using peptides for Env labeling revealed structural dynamics of Env, but peptide use risks potential effects on structural integrity/dynamics. While incorporating noncanonical amino acids (ncAAs) into Env by amber stop-codon suppression, followed by click chemistry, offers a minimally invasive approach, this has proved to be technically challenging for HIV-1...
January 8, 2024: Cell Chemical Biology
https://read.qxmd.com/read/38227281/nucleobase-functionalized-7-deazaisoguanine-and-7-deazapurin-2-6-diamine-nucleosides-halogenation-cross-coupling-and-cycloaddition
#18
JOURNAL ARTICLE
Zhenqiang Xia, Dasharath Kondhare, Somnath Shivaji Chandankar, Sachin A Ingale, Peter Leonard, Frank Seela
The functionalization in position-7 of 7-deazaisoguanine and 7-deazapurin-2,6-diamine ribo- and 2'-deoxyribonucleosides by halogen atoms (chloro, bromo, iodo), and clickable alkynyl and vinyl side chains for copper-catalyzed and copper-free cycloadditions is described. Problems arising during the synthesis of the 7-iodinated isoguanine ribo- and 2'-deoxyribonucleosides were solved by the action of acetone. The impact of side chains and halogen atoms on the p K a values and hydrophobicity of nucleosides was investigated...
January 16, 2024: Journal of Organic Chemistry
https://read.qxmd.com/read/38214216/lanthanide-complex-enhanced-bioorthogonal-branched-dna-amplification
#19
JOURNAL ARTICLE
Fang Zhao, Yunpeng Guan, Fei Su, Zhongbo Du, Shihui Wen, Le Zhang, Dayong Jin
Fluorescence in situ hybridization (FISH) is a widely used technique for detecting intracellular nucleic acids. However, its effectiveness in detecting low-copy nucleic acids is limited due to its low fluorescence intensity and background autofluorescence. To address these challenges, we present here an approach of lanthanide-complex-enhanced bioorthogonal-branched DNA amplification (LEBODA) with high sensitivity for in situ nuclear acid detection in single cells. The approach capitalizes on two levels of signal amplification...
January 12, 2024: Analytical Chemistry
https://read.qxmd.com/read/38178719/a-versatile-method-for-conjugating-lipid-nanoparticles-on-t-cells-through-combination-of-click-chemistry-and-metabolic-glycoengineering
#20
JOURNAL ARTICLE
Xin Li, Sven Weller, Gael Clergeaud, Thomas L Andresen
Cell-mediated drug delivery by conjugating nanomedicine to the surface of living cells is a promising strategy for enhancing the efficacy of both drug delivery and cell therapy. It exploits the tissue homing properties of the specific cell types to overcome in vivo barriers and forms a drug depot by directly putting the therapeutic payload in target cells. An important concern of developing this system is the method to conjugate nanoparticles on cells. Herein, we developed a bioorthogonal T cell conjugation strategy using SPAAC click chemistry, which allows controllable and highly efficient conjugation without affecting the viability and functions of the cytotoxic T lymphocytes...
January 4, 2024: Biotechnology Journal
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