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Fatty acid binding protein

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https://www.readbyqxmd.com/read/29164883/raspberry-ketone-reduced-lipid-accumulation-in-3t3-l1-cells-and-ovariectomy-induced-obesity-in-wistar-rats-by-regulating-autophagy-mechanisms
#1
Sy-Ying Leu, Yi-Chen Chen, Yung-Chieh Tsai, Yao-Wen Hung, Chih-Hsiung Hsu, Yen-Mei Lee, Pao-Yun Cheng
This study aimed to determine the anti-obesity effects of raspberry ketone (RK), one of the major aromatic compounds contained in raspberry, and its underlying mechanisms. During adipogenesis of 3T3-L1 cells, RK (300 μM) significantly reduced lipid accumulation and downregulated the expression of CCAAT/enhancer-binding protein α (C/EBPα), peroxisome proliferation-activated receptor γ (PPARγ), fatty acid-binding protein 4 (FABP4), and fatty acid synthase (FAS). RK also reduced the expression of light chain 3B (LC3B), autophagy-related protein 12 (Atg12), sirtuin 1 (SIRT1), and phosphorylated-tuberous sclerosis complex 2 (TSC2), whereas it increased the level of p62 and phosphorylated-mammalian target of rapamycin (mTOR)...
November 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29162722/docosahexaenoic-acid-lowers-cardiac-mitochondrial-enzyme-activity-by-replacing-linoleic-acid-in-the-phospholipidome
#2
E Madison Sullivan, Edward Ross Pennington, Genevieve C Sparagna, Maria J Torres, P Darrell Neufer, Mitchel Harris, James Washington, Ethan J Anderson, Tonya N Zeczycki, David A Brown, Saame Raza Shaikh
Cardiac mitochondrial phospholipid acyl chains regulate respiratory enzymatic activity. In several diseases, the rodent cardiac phospholipidome is extensively rearranged; however, whether specific acyl chains impair respiratory enzyme function is unknown. One unique remodeling event in the myocardium of obese and diabetic rodents is an increase in docosahexaenoic acid (DHA) levels. Here, we first confirmed that cardiac DHA levels are elevated in diabetic humans relative to controls. We then used dietary supplementation of a Western diet with DHA as a tool to promote cardiac acyl chain remodeling and to study its influence on respiratory enzyme function...
November 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29162011/skeletal-muscle-injury-biomarkers-assay-qualification-efforts-and-translation-to-the-clinic
#3
Richard A Goldstein
Skeletal muscle (SKM) injury or myopathy results in structural or functional defects in SKMs that can be caused by variety of factors such as (1) genetic, (2) drug-induced, (3) disease progression (cachexia), or (4) aging (sarcopenia). Creatine kinase (CK) and aspartate transaminase (AST) activity assays have been routinely used as SKM injury biomarkers, but they lack sensitivity and tissue specificity. In collaboration with the Predictive Safety Testing Consortium, we evaluated the diagnostic performance of a muscle injury biomarker panel (MIP) compared to CK and AST and their correlation with the histology scores across 34 different rat studies...
October 2017: Toxicologic Pathology
https://www.readbyqxmd.com/read/29161996/computational-screening-of-fatty-acid-synthase-inhibitors-against-thioesterase-domain
#4
Wanwisa Panman, Bodee Nutho, Supakarn Chamni, Supaporn Dokmaisrijan, Nawee Kungwan, Thanyada Rungrotmongkol
Thioesterase (TE) domain of fatty acid synthase (FAS) is an attractive therapeutic target for design and development of anticancer drugs. In this present work, we search for the potential FAS inhibitors of TE domain from the ZINC database based on similarity search using three natural compounds as templates, including flavonoids, terpenoids and phenylpropanoids. Molecular docking was used to predict the interaction energy of each screened ligand compared to the reference compound, which is methyl γ-linolenylfluorophosphonate (MGLFP)...
November 21, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29161081/diet-obesity-and-asthma
#5
Lisa G Wood
Obesity has reached epidemic proportions in many developed countries as Western dietary patterns have been widely adopted. These diets are characterized by excess energy intake as well as regular consumption of processed or "fast" foods and limited consumption of fruit, vegetables, and whole grains. The result is a high intake of saturated fat, refined carbohydrates, and sodium; and a low intake of fiber, vitamins, and other phytochemicals. This type of poor-quality diet has been associated with increased risk of chronic inflammatory diseases, including asthma...
November 2017: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/29160429/reduction-of-venous-pressure-during-the-resection-of-liver-metastases-compromises-enteric-blood-flow-igfbp-1-as-a-novel-biomarker-of-intestinal-barrier-injury
#6
Hermes Vieira Barbeiro, Marcel Autran César Machado, Heraldo Possolo de Souza, Fabiano Pinheiro da Silva, Marcel Cerqueira César Machado
OBJECTIVES: Disruption of the intestinal barrier and bacterial translocation commonly occur when intestinal blood flow is compromised. The aim of this study was to determine whether liver resection induces intestinal damage. METHODS: We investigated intestinal fatty-acid binding protein and insulin-like growth factor binding protein levels in the plasma of patients who underwent liver resection. RESULTS: We show that liver resection is associated with significant intestinal barrier injury, even if the Pringle maneuver is not performed...
October 2017: Clinics
https://www.readbyqxmd.com/read/29159775/toxic-effects-of-phytol-and-retinol-on-human-glioblastoma-cells-are-associated-with-modulation-of-cholesterol-and-fatty-acid-biosynthetic-pathways
#7
Gustavo Facchini, Raffaela Silvestre Ignarro, Erika Rodrigues-Silva, André Schwambach Vieira, Iscia Lopes-Cendes, Roger Frigério Castilho, Fabio Rogerio
Glioblastoma (GBM) is the most common primary brain tumor. Genetic mutations may reprogram the metabolism of neoplastic cells. Particularly, alterations in cholesterol and fatty acid biosynthetic pathways may favor biomass synthesis and resistance to therapy. Therefore, compounds that interfere with those pathways, such as phytol (PHY) and retinol (RET), may be appropriate for cytotoxic approaches. We tested the effect of PHY or RET on the viability of human GBM cell lines (U87MG, A172 and T98G). Since the compounds showed a dose-dependent cytotoxic effect, additional analyses were performed with IC50 values...
November 20, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29159216/data-on-the-decreased-expression-of-foxo1-by-mir-1271-in-hepg2-hepatocytes
#8
Won-Mo Yang, Kyung-Ho Min, Se-Whan Park, Wan Lee
Obesity and metabolic diseases are closely associated with insulin resistance. Obesity-induced miRNAs are also considered to be potential contributors to the development of insulin resistance and type 2 diabetes. Previously, the expression of miR-1271 was reported to be upregulated in the liver of diet-induced obese mice (Yang et al., 2016) [1]. In this data article, multiple in silico analysis predicted FOXO1 gene to be a direct target of miR-1271. Dual luciferase reporter gene analysis showed that miR-1271 suppressed FOXO1 expression by direct binding to 3'UTR...
December 2017: Data in Brief
https://www.readbyqxmd.com/read/29158261/the-biotin-ligating-protein-bpl-1-is-critical-for-lipid-biosynthesis-and-polarization-of-the-caenorhabditis-elegans-embryo
#9
Jason S Watts, Diane G Morton, Kenneth J Kemphues, Jennifer L Watts
Biotin is an essential cofactor for multiple metabolic reactions catalyzed by carboxylases. Biotin is covalently linked to apoproteins by holocarboxylase synthetase (HCS). Accordingly, some mutations in HCS cause holocarboxylase deficiency, a rare metabolic disorder that can be life threatening if left untreated. However, the long-term effects of HCS deficiency are poorly understood. Here, we report our investigations of bpl-1, which encodes the C. elegans ortholog of HCS. We found that mutations in the biotin-binding region of bpl-1 are maternal-effect lethal, and cause defects in embryonic polarity establishment, meiosis, and the integrity of the eggshell permeability barrier...
November 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29158255/nitro-fatty-acid-inhibition-of-triple-negative-breast-cancer-cell-viability-migration-invasion-and-tumor-growth
#10
Chen-Shan Chen Woodcock, Yi Huang, Steven R Woodcock, Sonia R Salvatore, Bhupinder Singh, Franca Golin-Bisello, Nancy E Davidson, Carola Neumann, Bruce A Freeman, Stacy G Wendell
Triple negative breast cancer (TNBC) comprises ~20% of all breast cancers and is the most aggressive mammary cancer subtype. Devoid of the estrogen and progesterone receptors, along with the receptor tyrosine kinase ERB2 (HER2) that define most mammary cancers, there are no targeted therapies for patients with TNBC. This, combined with a high metastatic rate and a lower 5-year survival rate than for other breast cancer phenotypes, means there is significant unmet need for new therapeutic strategies. Herein, the anti-neoplastic effects of the electrophilic fatty acid nitroalkene derivative, 10-nitro-octadec-9-enoic acid (nitro-oleic acid, NO2-OA), were investigated in multiple preclinical models of TNBC...
November 20, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29158087/fabp4-induces-asthmatic-airway-epithelial-barrier-dysfunction-via-ros-activated-foxm1
#11
Gaohui Wu, Liteng Yang, Yi Xu, Xiaohong Jiang, Xiaomin Jiang, Lisha Huang, Ling Mao, Shaoxi Cai
Functional abnormal airway epithelial cells, along with activated inflammatory cells, resulting in chronic airway inflammation, are considered as the characteristic of asthma. Fatty Acid Binding Protein 4 (FABP4) takes part in glucose and lipid homeostasis, and also have an important role in allergic airway inflammation. However, whether FABP4 influence barrier function of airway epithelial cells is unknown. In vivo, a HDM-induced murine model of asthma was obtained to assessed airway inflammation and protein expression of E-cadherin and Forkhead Box M1 (FoxM1)...
November 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29156529/alpinetin-improved-high-fat-diet-induced-non-alcoholic-fatty-liver-disease-nafld-through-improving-oxidative-stress-inflammatory-response-and-lipid-metabolism
#12
Yong Zhou, Yin-Lu Ding, Jian-Liang Zhang, Peng Zhang, Jin-Qing Wang, Zhao-Hua Li
The non-alcoholic fatty liver disease (NAFLD) has become a serious medical problem and an increasing threat to public health. It is characterized by the abnormal fat accumulation in liver without excessive alcohol intake. The concurrent NAFLD might up-regulate the risk of chronic kidney disease as well as the mortality rate. Though various drugs have been investigated to attenuate NAFLD, further study is still necessary to find new therapeutic strategy and to reveal the underlying molecular mechanism. In the present study, NAFLD animal models were induced by feeding with high fat (HF) diet for 8 weeks...
November 15, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29155746/saturated-fatty-acids-induce-ceramide-associated-macrophage-cell-death
#13
Yuwen Zhang, Jiaqing Hao, Yanwen Sun, Bing Li
Macrophages highly express epidermal fatty acid-binding protein and adipose fatty acid-binding protein. They actively uptake saturated and unsaturated fatty acids, which might play a critical role in regulating their immune functions. Numerous studies have shown that various fatty acids, saturated or unsaturated, may possess different impacts on cell growth and function. However, the approaches used for fatty acid preparation vary, which may lead to non-physiological results. Serum albumin, a natural carrier for fatty acids in mammalian peripheral blood, is recommended for forming a conjugate complex with the sodium salt of fatty acids to study fatty acid function in mammalian cells, thus minimizing the toxicity of fatty acid soap...
October 31, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29152653/elaidic-acid-induces-cell-apoptosis-through-induction-of-ros-accumulation-and-endoplasmic-reticulum-stress-in-sh%C3%A2-sy5y-cells
#14
Wei-Wei Ma, Lei Zhao, Lin-Hong Yuan, Huan-Ling Yu, Hui Wang, Xin-Yuan Gong, Feng Wei, Rong Xiao
Elaidic acid, which is a major trans fatty acid, has been reported to be involved in neurotoxicity; however, the underlying molecular mechanisms underlying its neurotoxic effects remain largely unknown. Therefore, the present study aimed to investigate the potential mechanisms underlying elaidic acid‑induced neuronal damage in vitro. The SH‑SY5Y neuroblastoma cell line was used as a model in the present study. Following treatment of cells with various concentrations of elaidic acid or with vehicle for 24 h, cell viability was measured using the MTT assay...
October 19, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29152559/fasciola-hepatica-esps-could-indistinctly-activate-or-block-multiple-toll-like-receptors-in-a-human-monocyte-cell-line
#15
Olgary Figueroa-Santiago, Ana M Espino
Fasciola hepatica is a parasitic helminth that induces Th2/Treg responses in its mammalian host. Some reports have suggested that ESPs achieve these polarized immune responses by delaying the activation of dendritic cells and macrophages during the early stages of innate immunity, a process that is mediated by TLR4. The present study aimed to investigate whether TLRs other than TLR4 could also be targeted by F. hepatica ESPs. To achieve this aim a screening system was optimized using THP1-Blue CD14 cells. ESPs were first separated based on their molecular weight and according their net charge by ion exchange chromatography (IEC)...
2017: Ann Clin Pathol
https://www.readbyqxmd.com/read/29152099/s149r-a-novel-mutation-in-the-abcd1-gene-causing-x-linked-adrenoleukodystrophy
#16
Fang Yan, Wenbo Wang, Hui Ying, Hongyu Li, Jing Chen, Chao Xu
X-linked adrenoleukodystrophy (X-ALD) is the most common peroxisomal disorder. It is a heterogeneous disorder caused by mutations in the ATP-binding cassette protein subfamily D1 (ABCD1) gene, encoding the peroxisomal membrane protein ALDP, which is involved in the transmembrane transport of very long-chain fatty acids. For the first time, we report a case of olivopontocerebellar X-ALD on the Chinese mainland. In this study, a novel mutation (c.447T>A; p.S149R) in ABCD1 was detected in a patient diagnosed with X-ALD...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151415/-diagnostic-value-of-citrulline-and-intestinal-fatty-acid-binding-protein-on-acute-gastrointestinal-injury-in-critical-patients-a-prospective-study-of-530-patients
#17
Jing Wang, Limei Yu, Yonghong Xia, Yanling Gao, Wenbo Yu, Haihong Sun, Yizhu Sun
OBJECTIVE: To observe the incidence of acute gastrointestinal injury (AGI) in intensive care unit (ICU) patients, and to approach the value of serum citrulline and intestinal fatty acid binding protein (IFABP) on diagnosis of AGI in critical patients. METHODS: A prospective study was conducted. 576 critical patients admitted to ICU of Yantai Yuhuangding Hospital from February 2016 to February 2017 were enrolled. According to the AGI classification proposed by European Society of Intensive Care Medicine (ESICM) in 2012, the AGI and severity of the patients were observed...
November 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29151385/the-compositional-and-metabolic-responses-of-gilthead-seabream-sparus-aurata-to-a-gradient-of-dietary-fish-oil-and-associated-n-3-long-chain-pufa-content
#18
Sam J S Houston, Vasileios Karalazos, John Tinsley, Mónica B Betancor, Samuel A M Martin, Douglas R Tocher, Oscar Monroig
The replacement of fish oil (FO) with vegetable oil (VO) in feed formulations reduces the availability of n-3 long-chain PUFA (LC-PUFA) to marine fish such as gilthead seabream. The aim of this study was to examine compositional and physiological responses to a dietary gradient of n-3 LC-PUFA. Six iso-energetic and iso-nitrogenous diets (D1-D6) were fed to seabream, with the added oil being a blend of FO and VO to achieve a dietary gradient of n-3 LC-PUFA. Fish were sampled after 4 months feeding, to determine biochemical composition, tissue fatty acid concentrations and lipid metabolic gene expression...
November 20, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/29148052/influence-of-the-addition-of-soy-product-and-wheat-fibre-on-rheological-textural-and-other-quality-characteristics-of-pizza
#19
Virginia Glicerina, Federica Balestra, Francesco Capozzi, Marco Dalla Rosa, Santina Romani
The effect of partial replacement of wheat flour with soy-paste and wheat fibre on rheological, textural, physico-chemical and organoleptic characteristics of an enriched pizza base (E) was investigated in comparison with those of a control pizza base (C). New ingredients (e.g. enriched cooked ham, whey cheese and tomato sauce realized using food industry by-products) were also used in E pizza topping to further increase its nutritional properties. Enriched dough was developed first at a laboratory level. Large and small deformation, moisture, leavening activity and metabolic heat were tested...
November 17, 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/29144270/alkaline-phosphatase-in-infant-cardiopulmonary-bypass-kinetics-and-relationship-to-organ-injury-and-major-cardiovascular-events
#20
Jesse A Davidson, Tracy T Urban, Christine Baird, Suhong Tong, Alan Woodruff, Mark Twite, James Jaggers, Eric A F Simões, Paul Wischmeyer
OBJECTIVES: To determine the kinetics of alkaline phosphatase (AP) activity and concentration after infant cardiopulmonary bypass, including isoform-specific changes, and to measure the association between postoperative AP activity and major postoperative cardiovascular events, organ injury/dysfunction, and postoperative support requirements STUDY DESIGN: Prospective cohort study of 120 infants ≤120 days of age undergoing cardiopulmonary bypass. AP total and isoform-specific activity was assessed at 6 time points (preoperation, rewarming, 6, 24, 48, and 72 hours postoperation)...
November 2017: Journal of Pediatrics
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