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Fatty acid binding protein

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https://www.readbyqxmd.com/read/28810950/clofibrate-attenuates-ros-production-by-lipid-overload-in-cultured-rat-hepatoma-cells
#1
Yufei Chen, Wei Li, Guqi Wang, Frank J Burczynski
PURPOSE: To investigate the effect of clofibrate on inducing liver fatty acid binding protein (FABP1) following a high-fat load in a hepatocyte cell culture model. METHODS: Rat hepatoma cells (CRL-1548) were treated with a fatty acid (FA) mixture consisting of oleate:palmitate (2:1) in the presence of 3% albumin. Cells were treated with 0, 0.5, 1, 2, or 3 mM FA for 24 and 48 hr, or further treated with 500 µM clofibrate (CLO) to induce FABP1 levels. Cytotoxicity was determined using the WST-1 assay...
2017: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28810126/combined-virtual-screening-and-substructure-search-for-discovery-of-novel-fabp4-inhibitors
#2
Haiyan Cai, Ting Wang, Zhuo Yang, Zhijian Xu, Guimin Wang, Heyao Wang, Weiliang Zhu, Kaixian Chen
Fatty acid-binding protein 4 (FABP4, AFABP) is a potential drug target for diabetes and atherosclerosis. In this study, a series of novel FABP4 inhibitors were discovered through combining virtual screening and substructure search. Seventeen compounds exhibited FABP4 inhibitory activities with IC50 <10 μM, among which eleven compounds showed high selectivity against FABP3. The best compound 36b displayed an IC50 value of 1.5 μM. Molecular docking and point mutation studies revealed that Gln95, Arg126 and Tyr128 play key roles for these compounds binding with FABP4...
August 15, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28808299/gut-homing-%C3%AE-42pd1-v%C3%AE-2-t-cells-promote-innate-mucosal-damage-via-tlr4-during-acute-hiv-type-1-infection
#3
Allen Ka Loon Cheung, Hau-Yee Kwok, Yiru Huang, Min Chen, Yufei Mo, Xilin Wu, Ka-Shing Lam, Hoi-Kuan Kong, Terrence Chi Kong Lau, Jingying Zhou, Jingjing Li, Lin Cheng, Boon Kiat Lee, Qiaoli Peng, Xiaofan Lu, Minghui An, Hui Wang, Hong Shang, Boping Zhou, Hao Wu, Aimin Xu, Kwok-Yung Yuen, Zhiwei Chen
The innate immune cells underlying mucosal inflammatory responses and damage during acute HIV-1 infection remain incompletely understood. Here, we report a Vδ2 subset of gut-homing γδ T cells with significantly upregulated Δ42PD1 (a PD1 isoform) in acute (~20%) HIV-1 patients compared to chronic HIV-1 patients (~11%) and healthy controls (~2%). The frequency of Δ42PD1(+)Vδ2 cells correlates positively with plasma levels of pro-inflammatory cytokines and fatty-acid-binding protein before detectable lipopolysaccharide in acute patients...
August 14, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28808264/knockdown-of-lncrna-mir31hg-inhibits-adipocyte-differentiation-of-human-adipose-derived-stem-cells-via-histone-modification-of-fabp4
#4
Yiping Huang, Chanyuan Jin, Yunfei Zheng, Xiaobei Li, Shan Zhang, Yixin Zhang, Lingfei Jia, Weiran Li
Adipogenesis plays an important role in the regulation of whole-body energy homeostasis and is inextricably related to obesity. Several studies have highlighted the relevance of microRNAs in adipocyte differentiation, but the contributions of long non-coding RNAs (lncRNAs) are still largely uncharacterized. Here, we determined that lncRNA MIR31HG is related to adipocyte lineage commitment. We demonstrated that knockdown of MIR31HG inhibited adipocyte differentiation, whereas overexpression of MIR31HG promoted adipogenesis in vitro and in vivo...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28808132/defining-electron-bifurcation-in-the-electron-transferring-flavoprotein-family
#5
Amaya M Garcia Costas, Saroj Poudel, Anne-Frances Miller, Gerrit J Schut, Rhesa N Ledbetter, Kathryn R Fixen, Lance C Seefeldt, Michael W W Adams, Caroline S Harwood, Eric S Boyd, John W Peters
Electron bifurcation is the coupling of exergonic and endergonic redox reactions to simultaneously generate (or utilize) low and high potential electrons. It is the third recognized form of energy conservation in biology and has recently been described in select electron transferring flavoproteins (Etfs). Etfs are flavin-containing heterodimers best known for donating electrons derived from fatty acid and amino acid oxidation to an electron transfer respiratory chain via ETF quinone oxidoreductase. Canonical examples contain a flavin adenine dinucleotide (FAD) that is involved in electron transfer as well as a non-redox active adenosine monophosphate (AMP)...
August 14, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28807601/ubx-domain-containing-proteins-are-involved-in-lipid-homeostasis-and-stress-responses-in-pichia-pastoris
#6
Meng Zhang, Qilin Yu, Zhe Liu, Chen Liang, Biao Zhang, Mingchun Li
Ubiquitin regulatory X (UBX) domain-containing proteins constitute a family of proteins and are substrate adaptors of AAA ATPase Cdc48. UBX proteins can bind to the N-terminal region of Cdc48 to perform endoplasmic reticulum associated protein degradation (ERAD). In this study, we identified two UBX domain-containing proteins, Ubx1 and Ubx2, in Pichia pastoris and found that the two proteins could recover the growth defect of Saccharomyces cerevisiae in ubx2Δ. Our results revealed that Ubx1 and Ubx2 play critical roles in synthesis of unsaturated fatty acids by affecting Spt23...
August 11, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28807550/decreased-gene-expression-of-fatty-acid-binding-protein-3-in-the-atrium-of-patients-with-new-onset-of-atrial-fibrillation-in-cardiac-perioperative-phase
#7
Yasushige Shingu, Takashi Yokota, Shingo Takada, Haruki Niwano, Tomonori Ooka, Hiroki Katoh, Tsuyoshi Tachibana, Suguru Kubota, Yoshiro Matsui
BACKGROUND: Post-operative atrial fibrillation (POAF) frequently occurs after cardiac surgery. However, the mechanisms of POAF have not been fully elucidated. We aimed to examine whether pre-operative atrial gene expression related to cardiac metabolism is changed in patients with POAF. METHODS: Right atrial tissue was obtained during surgery from 38 patients who underwent cardiac surgery from 2013 to 2015. Atrial expression levels were determined by reverse transcription polymerase chain reaction for the following genes: glucose transporter type 4, peroxisome proliferator-activated receptor-α, fatty acid translocase, carnitine palmitoyltransferase 1B, and fatty acid binding protein 3 (FABP3)...
August 11, 2017: Journal of Cardiology
https://www.readbyqxmd.com/read/28803762/apigenin-protects-against-alcohol-induced-liver-injury-in-mice-by-regulating-hepatic-cyp2e1-mediated-oxidative-stress-and-ppar%C3%AE-mediated-lipogenic-gene-expression
#8
Feng Wang, Jin-Cheng Liu, Rui-Jun Zhou, Xi Zhao, Mei Liu, Hua Ye, Mei-Lin Xie
Alcohol is a major cause of liver injury, and there are currently no ideal pharmacological reagents that can prevent or reverse this disease. Apigenin is one of the most common flavonoids present in numerous plants and has many beneficial effects. But whether or not apigenin may protect against alcohol-induced liver injury remains unknown. Our aim was to examine the effect and potential mechanisms. The experimental mice were given 56% erguotou wine or simultaneously given apigenin 150-300 mg/kg by gavage for 30 days...
August 10, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28802701/acyl-modification-and-binding-of-mitochondrial-acp-to-multiprotein-complexes
#9
Heike Angerer, Stefan Schönborn, Jan Gorka, Ute Bahr, Michael Karas, Ilka Wittig, Juliana Heidler, Jan Hoffmann, Nina Morgner, Volker Zickermann
The mitochondrial acyl carrier protein (ACPM/NDUFAB1) is a central element of the mitochondrial fatty acid synthesis type II machinery. Originally ACPM was detected as a subunit of respiratory complex I but the reason for the association with the large enzyme complex remained elusive. Complex I from the aerobic yeast Yarrowia lipolytica comprises two different ACPMs, ACPM1 and ACPM2. They are anchored to the protein complex by LYR (leucine-tyrosine-arginine) motif containing protein (LYRM) subunits LYRM3 (NDUFB9) and LYRM6 (NDUFA6)...
August 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28802091/a-cyclic-and-intermittent-very-low-protein-diet-can-have-beneficial-effects-against-advanced-diabetic-nephropathy-in-wistar-fatty-fa-fa-rats-an-animal-model-of-type-2-diabetes-and-obesity
#10
Munehiro Kitada, Yoshio Ogura, Taeko Suzuki, Itaru Monnno, Keizo Kanasaki, Ai Watanabe, Daisuke Koya
A low-protein diet (LPD), particularly, very low-protein diet (VLPD) is expected for reno-protection in advanced chronic kidney disease, including diabetic nephropathy. We previously also demonstrated that a VLPD clearly improved advanced diabetic nephropathy in a type 2 diabetes and obesity rat. However, clinically, an everyday long-term VLPD contributes to poor adherence, which may be related to controvertial results of an LPD on the suppression for diabetic nephropathy, and has nutritional issues, such as sarcopenia or protein-energy wasting...
August 12, 2017: Nephrology
https://www.readbyqxmd.com/read/28801883/prognostic-utility-of-fatty-acid-binding-protein-4-in-patients-with-type-2-diabetes-and-acute-ischemic-stroke
#11
Siou Li, Pengxiang Bi, Weina Zhao, Yifei Lian, Haifu Zhu, Dan Xu, Jiayuan Ding, Quankui Wang, Changhao Yin
The role of fatty acid-binding proteins (FABPs) in atherosclerosis has been investigated. The aim of this study was to verify the hypothesis that higher levels of serum fatty acid-binding protein 4 (FABP4) could be a prognostic factor in Chinese patients with type 2 diabetes (T2DM) and acute ischemic stroke (AIS). From September 2015 to August 2016, consecutive first-ever AIS patients combined with T2DM were included in this study. FABP4, NIH stroke scale (NIHSS), and conventional risk factors were evaluated to determine their value to predict functional outcomes within 3 months...
August 11, 2017: Neurotoxicity Research
https://www.readbyqxmd.com/read/28800633/feeding-fasting-dependent-recruitment-of-membrane-microdomain-proteins-to-lipid-droplets-purified-from-the-liver
#12
Kritika Sadh, Priyanka Rai, Roop Mallik
Lipid droplets (LDs) are cellular stores of neutral fat that facilitate lipid and protein trafficking in response to metabolic cues. Unlike other vesicles, the phospholipid membrane on the LD is a monolayer. Interestingly, this monolayer membrane has free cholesterol, and may therefore contain lipid microdomains that serve as a platform for assembling proteins involved in signal transduction, cell polarity, pathogen entry etc. In support of this, cell culture studies have detected microdomain-associated "raftophilic" proteins on LDs...
2017: PloS One
https://www.readbyqxmd.com/read/28798415/epicatechin-oligomers-longer-than-trimers-have-anti-cancer-activities-but-not-the-catechin-counterparts
#13
Kohki Takanashi, Manato Suda, Kiriko Matsumoto, Chisato Ishihara, Kazuya Toda, Koichiro Kawaguchi, Shogo Senga, Narumi Kobayashi, Mikihiro Ichikawa, Miyuki Katoh, Yasunao Hattori, Sei-Ichi Kawahara, Koji Umezawa, Hiroshi Fujii, Hidefumi Makabe
Since procyanidins (oligomeric catechin or epicatechin) were reported to exhibit health benefits, much attention has been paid to the synthesis of these compounds, especially those that are longer than trimers. In the present study, syntheses of cinnamtannin A3 (epicatechin pentamer), A4 (epicatechin hexamer), catechin tetramer, pentamer, arecatannin A2 (epicatechin-epicatechin-epicatechin-catechin) and A3 (epicatechin-epicatechin-epicatechin-epicatechin-catechin) were achieved. The key reaction was a Lewis acid mediated equimolar condensation...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28797150/-expression-of-peroxisome-proliferator-activated-receptors-fatty-acid-binding-protein-4-in-placenta-and-their-correlations-with-the-prognosis-of-pre-eclampsia
#14
Y Li, W L Chen, L Liu, H Gu
Objective: To examine the mRNA and protein expression of peroxisome proliferator-activated receptors (PPAR) and fatty acid binding protein-4 (FABP-4) in placenta, and to investigate their correlation with the prognosis of pre-eclampsia. Methods: The data of 177 women who delivered from January 2013 to December 2015 in Chinese People's Liberation Army No.94 Hospital were collected. Among them, 60 cases were term pregnancy and not in labor (TN); 46 cases were term pregnancy with preeclampsia and not in labor (TPE); 42 cases were preterm pregnancy and not in labor (PN); 29 cases were preterm pregnancy with pre-eclampsia and not in labor (PPE)...
July 25, 2017: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/28793772/effect-of-dietary-lipids-on-endotoxemia-influences-postprandial-inflammatory-response
#15
Javier Lopez-Moreno, Sonia García-Carpintero, Rosa Jimenez-Lucena, Carmen Haro, Oriol A Rangel-Zúñiga, Ruth Blanco-Rojo, Elena Maria Yubero-Serrano, Francisco J Tinahones, Javier Delgado-Lista, Pablo Pérez-Martínez, Helen M Roche, Jose Lopez-Miranda, Antonio Camargo
Metabolic syndrome (MetS) results in postprandial metabolic alterations that predisposes to a state of chronic low-grade inflammation and increased oxidative stress. We aimed to assess the effect of the consumption of the quantity and quality of dietary fat on fasting and postprandial plasma lipopolysaccharides (LPS). A subgroup of 75 subjects with metabolic syndrome was randomized to receive 1 of 4 diets: HSFA, rich in saturated fat; HMUFA, rich in monounsaturated fat; LFHCC n-3, low-fat, rich in complex carbohydrate diet supplemented with n-3 polyunsaturated fatty acids; LFHCC low-fat, rich in complex carbohydrate diet supplemented with placebo, for 12 week each...
August 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28791741/molecular-dynamics-study-on-the-apo-and-holo-forms-of-5-lipoxygenase
#16
Juan Torras, Mauro Maccarrone, Enrico Dainese
Lipoxygenases (LOXs) are non-heme iron containing enzymes catalyzing the dioxygenation of polyunsaturated fatty acids. LOX catalytic activity depends on the presence of iron in the active site and the iron removal is also able to affect the membrane binding properties of the enzyme. Leukotrienes biosynthesis is initiated by the action of 5-LOX at the level of nuclear membrane and the mechanism of enzyme-membrane interaction is thought to involve structural flexibility and conformational changes at the level of the protein tertiary structure...
August 8, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28781090/fatty-acid-binding-protein-4-is-associated-with-stroke-risk-and-severity-in-patients-with-acute-ischemic-stroke
#17
Li Chang, Jianlong Zhang, Li Liu, Zhen Huang, Yingbo Han, Yanyan Zhu
OBJECTIVE: The role of fatty acid-binding proteins (FABPs) in atherosclerosis has been investigated. The aim of this study was to verify the hypothesis that higher levels of serum FABP4 could be a biomarker for stroke and associated with stroke severity in Chinese patients with ischemic stroke. METHODS: All consecutive patients with first-ever acute ischemic stroke from September 2015 to August 2016 were recruited to participate in the study. Serum FABP4 levels and routine tests were examined at admission...
July 19, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28781081/specific-interaction-of-the-human-mitochondrial-uncoupling-protein-1-with-free-long-chain-fatty-acid
#18
Linlin Zhao, Shuqing Wang, Qianli Zhu, Bin Wu, Zhijun Liu, Bo OuYang, James J Chou
The mitochondrial uncoupling protein 1 (UCP1) generates heat by causing proton leak across the mitochondrial inner membrane that requires fatty acid (FA). The mechanism by which UCP1 uses FA to conduct proton remains unsolved, and it is also unclear whether a direct physical interaction between UCP1 and FA exists. Here, we have shown using nuclear magnetic resonance that FA can directly bind UCP1 at a helix-helix interface site composed of residues from the transmembrane helices H1 and H6. According to the paramagnetic relaxation enhancement data and molecular dynamics simulation, the FA acyl chain appears to fit into the groove between H1 and H6 while the FA carboxylate group interacts with the basic residues near the matrix side of UCP1...
July 19, 2017: Structure
https://www.readbyqxmd.com/read/28777927/carbohydrate-and-protein-intake-during-exertional-heat-stress-ameliorates-intestinal-epithelial-injury-and-small-intestine-permeability
#19
Rhiannon M J Snipe, Anthony Khoo, Cecilia M Kitic, Peter Gibson, Ricardo J S Costa
BACKGROUND: Exertional-heat stress (EHS) disturbs the integrity of the gastrointestinal tract leading to endotoxaemia and cytokinaemia, which have symptomatic and health implications. This study aimed to determine the effects of carbohydrate and protein intake during EHS on gastrointestinal integrity, symptoms and systemic responses. METHODS: Eleven (male n=6, female n=5) endurance runners completed 2h running at 60% V̇O2max in 35°C ambient temperature on three occasions in randomised order, consuming water (WATER) or 15g glucose (GLUC) or energy-matched whey protein hydrolysate (WPH) before and every 20min during EHS...
August 4, 2017: Applied Physiology, Nutrition, and Metabolism, Physiologie Appliquée, Nutrition et Métabolisme
https://www.readbyqxmd.com/read/28774885/prebiotic-galacto-oligosaccharides-mitigate-the-adverse-effects-of-iron-fortification-on-the-gut-microbiome-a-randomised-controlled-study-in-kenyan-infants
#20
Daniela Paganini, Mary A Uyoga, Guus A M Kortman, Colin I Cercamondi, Diego Moretti, Tanja Barth-Jaeggi, Clarissa Schwab, Jos Boekhorst, Harro M Timmerman, Christophe Lacroix, Simon Karanja, Michael B Zimmermann
OBJECTIVE: Iron-containing micronutrient powders (MNPs) reduce anaemia in African infants, but the current high iron dose (12.5 mg/day) may decrease gut Bifidobacteriaceae and Lactobacillaceae, and increase enteropathogens, diarrhoea and respiratory tract infections (RTIs). We evaluated the efficacy and safety of a new MNP formula with prebiotic galacto-oligosaccharides (GOS) combined with a low dose (5 mg/day) of highly bioavailable iron. DESIGN: In a 4-month, controlled, double-blind trial, we randomised Kenyan infants aged 6...
August 3, 2017: Gut
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