Lijuan Hu, Zhirui Lin, Yanheng Wu, Juqin Dong, Bo Zhao, Yanbing Cheng, Peiyu Huang, Lihua Xu, Tianliang Xia, Dan Xiong, Hongbo Wang, Manzhi Li, Ling Guo, Elliott Kieff, Yixin Zeng, Qian Zhong, Musheng Zeng
The latent expression pattern of Epstein-Barr Virus (EBV) genes in nasopharyngeal carcinoma (NPC) has been extensively investigated, and the expression of several lytic genes in NPC has been reported. However, comprehensive information through EBV transcriptome analysis in NPC is limited. We performed paired-end RNA-seq to systematically and comprehensively characterize the expression of EBV genes in NPC tissue and C666-1 NPC cell line, which consistently carries EBV. In addition to the transcripts restricted to type II latency infection, the type III latency EBNA3s genes and a substantial number of lytic genes, such as BZLF1, BRLF1, and BMRF1, were detected through RNA-seq and were further verified in C666-1 cells and NPC tissue through realtime PCR...
March 2016: Frontiers of Medicine