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https://www.readbyqxmd.com/read/29146508/biomechanical-analysis-of-palmar-midcarpal-instability-and-treatment-by-partial-wrist-arthrodesis
#1
Sarah A Shiga, Frederick W Werner, Marc Garcia-Elias, Brian J Harley
PURPOSE: To create a biomechanical model of palmar midcarpal instability by selective ligament sectioning and to analyze treatment by simulated partial wrist arthrodesis. METHODS: Nine fresh-frozen cadaver arms were moved through 3 servohydraulic actuated motions and 2 passive wrist mobilizations. The dorsal radiocarpal, triquetrohamate, scaphocapitate, and scaphotrapeziotrapezoid ligaments were sectioned to replicate palmar midcarpal instability. Kinematic data for the scaphoid, lunate, and triquetrum were recorded before and after ligament sectioning and again after simulated triquetrohamate arthrodesis (TqHA) and radiolunate arthrodesis (RLA)...
November 14, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29142485/unexpected-inflammation-in-the-sympathetic-ganglia-in-thromboangiitis-obliterans
#2
Mahdi Farzadnia, Hassan Ravari, Mitra Masoudian, Narges Valizadeh, Bahare Fazeli
The aim of this study was to inspect more closely the microscopic and molecular sympathetic ganglia histology in thromboangiitis obliterans (TAO). The paraffin block and frozen RNAlater-treated tissue of the lumbar sympathetic ganglia of 19 TAO Caucasian male patients were evaluated. The gene expression of cluster of differentiation 4 (CD4) and cluster of differentiation 8 (CD8) markers in the frozen RNAlater-treated sympathetic ganglia tissues were evaluated by real-time polymerase chain reaction. Unexpectedly, lymphocyte infiltration was observed in all of the histological sections, ranging from scattered to moderate lymphocyte infiltration...
December 2017: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
https://www.readbyqxmd.com/read/29139613/nipple-sparing-mastectomy-as-treatment-for-patients-with-ductal-carcinoma-in%C3%A2-situ-a-10-year-follow-up-study
#3
Víctor Lago, Vincenzo Maisto, Julia Gimenez-Climent, Jose Vila, Carlos Vazquez, Rafael Estevan
The objective was to determine the 10-year oncological safety of nipple-sparing mastectomy (NSM) in patients diagnosed with ductal carcinoma in situ (DCIS). The use of NSM preserves the nipple-areola complex (NAC). As residual fibroglandular breast tissue can remain behind the spared NAC, its use for patient with breast cancer is controversial. The oncologic outcomes and complication rates after performing NSM compared to other techniques are still under debate and a concern when treating patients with breast cancer...
November 15, 2017: Breast Journal
https://www.readbyqxmd.com/read/29136624/value-of-intraoperative-cytological-and-pathological-sentinel-lymph-node-diagnosis-in-fertility-sparing-trachelectomy-for-early-stage-cervical-cancer
#4
Kenzo Sonoda, Hideaki Yahata, Kaoru Okugawa, Eisuke Kaneki, Tatsuhiro Ohgami, Masafumi Yasunaga, Shingo Baba, Yoshinao Oda, Hiroshi Honda, Kiyoko Kato
BACKGROUND AND OBJECTIVES: Trachelectomy, a fertility-sparing surgery for early-stage cervical cancer, can be performed only when there is no extrauterine extension present. Therefore, identifying the sentinel lymph nodes (SLNs) and using them to obtain an intraoperative pathologic diagnosis can provide information on the feasibility and safety of trachelectomy. Our aim was to assess the value of an intraoperative SLN diagnosis. METHODS: We retrospectively analyzed the accuracy of intraoperative imprint cytology and frozen-section examination in 201 patients at our institution in whom trachelectomy was planned...
November 15, 2017: Oncology
https://www.readbyqxmd.com/read/29132791/in%C3%A2-vitro-kinematic-assessment-of-a-hinged-elbow-orthosis-following-lateral-collateral-ligament-injury
#5
Ranita H Manocha, Graham J W King, James A Johnson
PURPOSE: Elbow lateral collateral ligament injuries (LCLI) are often managed with protected mobilization using a hinged elbow orthosis (HEO). The objective of this investigation was to determine the effectiveness of an HEO in stabilizing the elbow following LCLI. METHODS: Seven fresh-frozen cadaveric upper extremity specimens were studied using a custom simulator that enabled elbow motion via computer-controlled actuators and servomotors attached to relevant tendons...
November 11, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29132787/distal-biceps-brachii-tendon-transfer-for-re-establishing-extrinsic-finger-function-feasibility-study-in-cadavers
#6
Matthew D Welsch, Alexander D Mih, Brock D Reiter, Gregory A Merrell
PURPOSE: To determine the anatomic feasibility of transferring the biceps brachii tendon into either the extensor digitorum communis (EDC) or flexor digitorum profundus (FDP), determine the excursion imparted to EDC and FDP tendons after transfer, and compare the work capacity of the cadaver biceps to previously published data on the biceps as well as the recipient muscles by calculating the physiologic cross-sectional area (PCSA). METHODS: Four fresh-frozen cadaver shoulder-elbow-wrist specimens were used to measure tendon excursion that can be obtained with transfer of the distal biceps tendon into either the EDC or FDP...
November 11, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29132012/distinct-ezrin-truncations-differentiate-metastases-in-sentinel-lymph-nodes-from-unaffected-lymph-node-tissues-from-primary-breast-tumors-and-from-healthy-glandular-breast-tissues
#7
Claudia Röwer, Christian George, Toralf Reimer, Bernd Stengel, Anngret Radtke, Bernd Gerber, Michael O Glocker
BACKGROUND: Lymph node metastasis status is a prognostic factor for further lymph node involvement and for patient survival in breast cancer patients. Frozen section analysis of lymph nodes is a reliable method for detection of macro-metastases. However, this method is far less effective in detecting micro-metastases, requesting improved diagnostic procedures. METHODS: We investigated expression and truncation of ezrin in (i) sentinel lymph node metastases, (ii) unaffected axillary lymph nodes, (iii) primary breast tumors, and (iv) healthy glandular breast tissues using 2D gel electrophoresis, SDS-PAGE, and mass spectrometry in addition to Western blotting...
November 10, 2017: Translational Oncology
https://www.readbyqxmd.com/read/29130199/in-situ-detection-of-microrna-expression-with-rnascope-probes
#8
Viravuth P Yin
Elucidating the spatial resolution of gene transcripts provides important insight into potential gene function. MicroRNAs are short, singled-stranded noncoding RNAs that control gene expression through base-pair complementarity with target mRNAs in the 3' untranslated region (UTR) and inhibiting protein expression. However, given their small size of ~22- to 24-nt and low expression levels, standard in situ hybridization detection methods are not amendable for microRNA spatial resolution. Here, I describe a technique that employs RNAscope probe design and propriety amplification technology that provides simultaneous single molecule detection of individual microRNA and its target gene...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130197/detection-of-mrna-and-associated-molecules-by-ish-iem-on-frozen-sections
#9
Catherine Rabouille
The use of tagged RNA probes to directly hybridize frozen sections of chemically fixed tissues, followed by the tag detection with specific antibodies and gold conjugates form the core of the in situ hybridization (ISH)-immunoelectron microscopy (IEM) method that we have developed and successfully used to detect endogenous gurken and bicoid mRNAs in Drosophila oocytes.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29130192/lcm-seq-a-method-for-spatial-transcriptomic-profiling-using-laser-capture-microdissection-coupled-with-polya-based-rna-sequencing
#10
Susanne Nichterwitz, Julio Aguila Benitez, Rein Hoogstraaten, Qiaolin Deng, Eva Hedlund
LCM-seq couples laser capture microdissection of cells from frozen tissues with polyA-based RNA sequencing and is applicable to single neurons. The method utilizes off-the-shelf reagents and direct lysis of the cells without RNA purification, making it a simple and relatively cheap method with high reproducibility and sensitivity compared to previous methods. The advantage with LCM-seq is also that tissue sections are kept intact and thus the positional information of each cell is preserved.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29127653/development-of-a-3d-workspace-shoulder-assessment-tool-incorporating-electromyography-and-an-inertial-measurement-unit-a-preliminary-study
#11
Navid Aslani, Siamak Noroozi, Philip Davenport, Richard Hartley, Mihai Dupac, Philip Sewell
Traditional shoulder range of movement (ROM) measurement tools suffer from inaccuracy or from long experimental setup times. Recently, it has been demonstrated that relatively low-cost wearable inertial measurement unit (IMU) sensors can overcome many of the limitations of traditional motion tracking systems. The aim of this study is to develop and evaluate a single IMU combined with an electromyography (EMG) sensor to monitor the 3D reachable workspace with simultaneous measurement of deltoid muscle activity across the shoulder ROM...
November 11, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29126039/the-resistance-to-failure-of-spring-ligament-reconstruction
#12
Chandra Pasapula, Adam Devany, Nicole C Fischer, Coen A Wijdicks, Tim Hübner, Franziska Reifenscneider, Sajid Shariff
INTRODUCTION: The spring ligament (SL) is increasingly recognised as the major structure that fails in acquired adult flatfoot deformity (AAFD). This is the first study that demonstrates integrity of repair of the SL. PATIENTS AND METHODS: Six pairs of fresh frozen cadavers were setup in a standardised fashion with ankle in plantargrade (mean age 59 years, BMI 25). A 25N lateral force was applied to the medial metatarsal head using an algometer. Lateral displacement of the foot was measured with SL intact, sectioned, following FibreWire(®) repair, then Arthrex InternalBrace (IB) reconstruction, then with selective sectioning of each limb of the IB reconstruction...
June 3, 2017: Foot
https://www.readbyqxmd.com/read/29122426/anatomical-factors-contributing-to-radial-nerve-excursion-at-the-brachium-a-cadaveric-study
#13
Wayne A Chen, T David Luo, Michael D Wigton, Zhongyu Li
PURPOSE: The radial nerve appears to be more vulnerable to injury in the brachium than the median and ulnar nerves. The underlying mechanism for this increased vulnerability is not well explained. We hypothesize that the radial nerve has less excursion than the median and ulnar nerves because it is anatomically tethered by the lateral intermuscular septum (LIS) and that elbow positioning and LIS release will improve its excursion. METHODS: Eight paired fresh-frozen cadaveric upper extremity specimens were used...
November 7, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29116514/combine-phage-antibody-display-library-selection-on-patient-tissue-specimens-with-laser-capture-microdissection-to-identify-novel-human-antibodies-targeting-clinically-relevant-tumor-antigens
#14
Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu
A functional approach to generate tumor-targeting human monoclonal antibodies is through selection of phage antibody display libraries directly on tumor cells. Although technically convenient, the use of cancer cell lines for the selection has limitations as those cell lines often undergo genetic and epigenetic changes during prolonged in vitro culture and alter their cell surface antigen expression profile. The key is to develop a technology that allows selection of phage antibody display libraries on tumor cells in situ residing in their natural tissue microenvironment...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29104611/frozen-section-evaluation-of-sentinel-lymph-nodes-in-breast-carcinoma-a-retrospective-analysis
#15
Leonardo Russo, Luis Betancourt, Gabriel Romero, Alí Godoy, Laura Bergamo, Rafael Delgado, Ángela Ruiz, Marianna Gutiérrez, Eduardo Salas, Maria Puzzi
Objective: To determine the false-negative rate, sensitivity, and diagnostic accuracy of the frozen section analysis of the sentinel lymph node (SLN) biopsy in early-stage breast cancer compared to the definitive section and to identify the factors that could be associated with the appearance of false-negative cases. Secondarily, to evaluate the pathological results of cases submitted to completion axillary lymph node dissection (ALND) for positive SLN. Methods: We performed a five-year review of cases (2011-2015), including patients with early-stage breast cancer undergoing SLN biopsy, with frozen section evaluation and subsequent definitive pathological analysis...
2017: Ecancermedicalscience
https://www.readbyqxmd.com/read/29101569/intra-corporeal-hand-sewn-esophagojejunostomy-is-a-safe-and-feasible-procedure-for-totally-laparoscopic-total-gastrectomy-short-term-outcomes-in-100-consecutive-patients
#16
Xiaowu Xu, Chaojie Huang, Yiping Mou, Renchao Zhang, Yu Pan, Ke Chen, Chao Lu
BACKGROUND: An optimal method for intracorporeal esophagojejunostomy has not yet been standardized. This study sought to introduce intracorporeal hand-sewn end-to-side esophagojejunostomy after totally laparoscopic total gastrectomy. METHODS: The author conducted a consecutive series of 100 intracorporeal hand-sewn esophagojejunostomies after totally laparoscopic total gastrectomy for upper third gastric cancer from September 2012 to December 2016. RESULTS: All patients were successfully operated on without conversion to open- or laparoscope-assisted surgery...
November 3, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/29100452/clinical-significance-of-mskcc-nomogram-on-guiding-the-application-of-touch-imprint-cytology-and-frozen-section-in-intraoperative-assessment-of-breast-sentinel-lymph-nodes
#17
Lisha Sun, Guanglei Chen, Yizhen Zhou, Lei Zhang, Zining Jin, Weiguang Liu, Guangping Wu, Feng Jin, Kai Li, Bo Chen
The widely practiced intra-operative methods for rapid evaluation and detection of sentinel lymph node (SLN) status include frozen section (FS) and touch imprint cytology (TIC). This study optimized the use of TIC and FS in the intra-operative detection of breast SLNs based on the Memorial Sloan Kettering Cancer Center (MSKCC) nomogram. Three hundred forty-two SLNs were removed from 79 patients. SLN metastatic probability was assessed by the MSKCC nomogram. The SLNs underwent intra-operative TIC and FS, as well as routine post-operative paraffin sections (RPSs)...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29098421/what-is-the-accuracy-of-frozen-section-in-the-diagnosis-of-mucinous-ovarian-tumours-a-9-year-review-of-performance-in-a-greek-tertiary-referral-centre
#18
Anastasios Tranoulis, Nikolaos Thomakos, Maria Sotiropoulou, Alexandros Rodolakis
PURPOSE: This retrospective study aimed to evaluate the diagnostic accuracy of the intra-operative frozen sections (FS) of mucinous ovarian tumours (mOT). METHODS: Between 2007 and 2015, a total of 105 mucinous ovarian samples were collected during laparotomy. The intra-operative FS accuracy was evaluated and potential factors correlated with increased inaccuracy assessed using both univariate and multivariate analysis. RESULTS: The overall FS accuracy was 82...
November 2, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29097686/proof-of-concept-raman-spectroscopy-study-aimed-to-differentiate-thyroid-follicular-patterned-lesions
#19
Julietta V Rau, Marco Fosca, Valerio Graziani, Chiara Taffon, Massimiliano Rocchia, Marco Caricato, Paolo Pozzilli, Andrea Onetti Muda, Anna Crescenzi
Inter-observer variability and cancer over-diagnosis are emerging clinical problems, especially for follicular patterned thyroid lesions. This challenge strongly calls for a new clinical tool to reliably identify neoplastic lesions and to improve the efficiency of differentiation between benign and malignant neoplasms, especially considering the increased diagnosis of small carcinomas and the growing number of thyroid nodules. In this study, we employed a Raman spectroscopy (RS) microscope to investigate frozen thyroid tissues from fourteen patients with thyroid nodules...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29096564/management-of-peripartum-hysterectomies-performed-on-patients-with-placenta-percreta-in-a-tertiary-central-hospital
#20
Sibel Sak, Mert Barut, Adnan Incebiyik, Hacer Uyanikoglu, Nese Hilali, Muhammet Sak
OBJECTIVE: The main objective of this study is to analyse the circumstances and management of peripartum hysterectomies performed on patients with placenta percreta. METHODS: This study included 37 patients who were diagnosed with placenta percreta, a condition in which the placenta invades the uterine wall, and who therefore underwent a peripartum hysterectomy. Their demographic characteristics, history of past caesareans, uterine surgery and curettage, duration of hospitalization, admission to an intensive care unit, neonatal outcomes, skin and uterus incision type, hypogastric artery ligation (HAL), complications, quantities of transfused ES (erythrocyte suspensions), and FFP (fresh frozen plasma), maternal morbidity and mortality and postoperative pathology results were retrospectively reviewed...
November 2, 2017: Journal of Maternal-fetal & Neonatal Medicine
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