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https://www.readbyqxmd.com/read/28316221/-value-of-jugulo-omohyoid-lymph-nodes-in-predicting-lateral-cervical-occult-metastasis-in-patients-with-papillary-thyroid-carcinoma
#1
C M An, Y Wang, S X Wang, Y L Yin, M Q Chen, Z G Xu, P Z Tang, Z J Li
Objective: To investigate the value of jugulo-omohyoid lymph nodes (JOHLN) in predicting occult lateral cervical lymph node metastasis in patients with papillary thyroid carcinoma (PTC). Methods: The clinicopathological data of 136 out of 2 100 PTC patients, who had a high risk of lateral neck lymph node metastasis and treated by us from January 2010 to December 2015, were retrospectively analyzed. Super selective neck dissection (SSND, level Ⅲ and Ⅳ)was performed and JOHLNs were sent for frozen section in all the 136 cases...
March 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28315641/development-of-quantitative-real-time-pcr-assays-for-postmortem-detection-of-mycobacterium-spp-common-in-zebrafish-danio-rerio-research-colonies
#2
Danielle M Meritet, Donna M Mulrooney, Michael L Kent, Christiane V Löhr
Mycobacterium spp. infections are common in zebrafish kept in research facilities. These comorbidities can substantially modulate the responses of these fish to external and internal stimuli. Therefore, diagnostic tests to detect Mycobacterium spp. infections in zebrafish colonies prove essential. Here, we outline the development of quantitative simplex real-time PCR assays to detect the 3 Mycobacterium species most commonly identified in laboratory zebrafish. The assays targeted the heatshock protein 65 gene of M...
March 1, 2017: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/28315499/evaluation-of-the-decision-making-process-in-the-conservative-approach-to-small-testicular-masses
#3
Andrea Benelli, Virginia Varca, Lorenzo Derchi, Andrea Gregori, Giorgio Carmignani, Alchiede Simonato
INTRODUCTION: We evaluate the clinical outcome of patients treated with conservative approach for small testicular masses (STMs). We analyzed the steps who brought to the selection of the therapeutic approach: starting from clinical presentation, through imaging and lab studies. METHODS: We considered 18 patients who underwent an organ-sparing approach for STMs from 2005 until 2014. The selection criteria were dimension of the mass and absence of clinical, laboratory and/or radiological malignancy suspicion...
March 15, 2017: Urologia
https://www.readbyqxmd.com/read/28314887/mechanical-strains-passing-through-the-acetabular-labrum-modify-its-shape-during-hip-motion-an-anatomical-study
#4
Matthieu Ollivier, Thomas Le Corroller, Sebastien Parratte, Patrick Chabrand, Jean-Noël Argenson, Olivier Gagey
PURPOSE: The function of the hip labrum during hip motion remains poorly known. Our hypothesis was that acetabular labrum will deform and change its shape during adduction to abduction movement consecutively to variation of strains passing from the acetabulum to the femoral head. An ex vivo anatomical study was conducted to analyse the morphological parameters variation of the mid-portion of the labrum (length, thickness, shape, deformation) as well as femoro-labral strains during hip adduction to abduction movement...
March 17, 2017: Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA
https://www.readbyqxmd.com/read/28301818/distribution-of-hemokinin-1-in-the-rat-trigeminal-ganglion-and-trigeminal-sensory-nuclear-complex
#5
Kaori Igawa, Hideki Funahashi, Yu Miyahara, Rumi Naono-Nakayama, Hisae Matsuo, Yoshihiro Yamashita, Sumio Sakoda, Toshikazu Nishimori, Yasushi Ishida
OBJECTIVE: A new mammalian tachykinin peptide encoded in a TAC4 gene was identified and designated as hemokinin-1 (HK-1). A representative of the tachykinin peptide family is substance P (SP), and the function of SP has been well characterized as a pain transmitter or modulator, while it is possible that HK-1 is involved in pruriceptive processing, but, as yet, the distribution of HK-1 peptide in the trigeminal sensory system is still unknown. Thus, the aim of the present study was to elucidate the distribution of HK-1, while comparing the expression of SP, in the trigeminal ganglion and trigeminal sensory nuclear complex...
March 6, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28299490/molecular-effects-of-fractional-ablative-erbium-yag-laser-treatment-with-multiple-stacked-pulses-on-standardized-human-three-dimensional-organotypic-skin-models
#6
Laurenz Schmitt, P M Amann, Y Marquardt, R Heise, K Czaja, P A Gerber, T Steiner, F Hölzle, Jens Malte Baron
The molecular changes in gene expression following ablative laser treatment of skin lesions, such as atrophic scars and UV-damaged skin, are not completely understood. A standardized in vitro model of human skin, to study the effects of laser treatment on human skin, has been recently developed. Therefore, the aim of the investigation was to examine morphological and molecular changes caused by fractional ablative erbium:YAG laser treatment on an in vitro full-thickness 3D standardized organotypic model of human skin...
March 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28293625/an-imaging-and-histological-study-on-intrahepatic-microvascular-passage-of-contrast-materials-in-rat-liver
#7
Qian Xia, Yuanbo Feng, Ting Yin, Yewei Liu, Guozhi Zhang, Jianjun Liu, Linjun Tong, Robin Willemyns, Jie Yu, Raymond Oyen, Gang Huang, Yicheng Ni
Background. Lipiodol has been applied for decades in transarterial chemoembolization to treat liver malignancies, but its intrahepatic pathway through arterioportal shunt (APS) in the liver has not been histologically revealed. This rodent experiment was conducted to provide evidence for the pathway of Lipiodol delivered through the hepatic artery (HA) but found in the portal vein (PV) and to elucidate the observed unidirectional APS. Methods. Thirty rats were divided into 5 groups receiving systemic or local arterial infusion of red-stained iodized oil (RIO) or its hydrosoluble substitute barium sulfate suspension (BSS), or infusion of BSS via the PV, monitored by real-time digital radiography...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28292895/diagnosis-of-prostate-cancer-by-desorption-electrospray-ionization-mass-spectrometric-imaging-of-small-metabolites-and-lipids
#8
Shibdas Banerjee, Richard N Zare, Robert J Tibshirani, Christian A Kunder, Rosalie Nolley, Richard Fan, James D Brooks, Geoffrey A Sonn
Accurate identification of prostate cancer in frozen sections at the time of surgery can be challenging, limiting the surgeon's ability to best determine resection margins during prostatectomy. We performed desorption electrospray ionization mass spectrometry imaging (DESI-MSI) on 54 banked human cancerous and normal prostate tissue specimens to investigate the spatial distribution of a wide variety of small metabolites, carbohydrates, and lipids. In contrast to several previous studies, our method included Krebs cycle intermediates (m/z <200), which we found to be highly informative in distinguishing cancer from benign tissue...
March 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28292525/intraoperative-cell-isolation-for-a-cytological-assessment-of-bone-resection-margins-in-patients-with-head-and-neck-cancer
#9
M Nieberler, P Häußler, M R Kesting, A Kolk, H Stimmer, K Nentwig, G Weirich, K-D Wolff
The intraoperative cytological assessment of bony resection margins (ICAB) is a feasible diagnostic approach to support frozen section for assessment of invasion of margins of soft and hard tissue. However, complex resection margins could challenge both diagnostic approaches. Our objective here was to identify the limitations of intraoperative diagnostic methods for assessing margins. We present an advanced cytological approach to assess complex margins that may solve the problem. Data from 119 patients in whom frozen section was supported by ICAB, were reviewed and the reasons for false results analysed...
March 11, 2017: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/28290238/ra-226-bioaccumulation-and-growth-indices-in-fish
#10
Xiaopei Shi, Richard Smith, Colin Seymour, Carmel Mothersill
PURPOSE: To determine the accumulated activity of Ra-226 in fathead minnows fed with environmentally relevant levels of Ra-226 for 5 months in water at 20 °C, and to evaluate the influence of this level of Ra-226 on the growth of fathead minnows. METHODS: Fathead minnows were fed with fish food containing 10-10,000 mBq/g Ra-226 for 5 months. At the end of the experiment, the fish were sacrificed, flash frozen in liquid nitrogen and kept at -20 °C. Longitudinal sections of 40 μm thickness were cut at the middle of the fish body using a cryostat...
March 14, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28286072/organ-sparing-surgery-in-penile-cancer-a-systematic-review
#11
REVIEW
Mohamed H Kamel, Nabil Bissada, Renee Warford, Judy Farias, Rodney Davis
PURPOSE: Although penile cancer (PC) only represents 1% of all male cancers, the traditional treatment of total or subtotal penectomy carries devastating psychological and functional outcomes. Organ sparing surgery (OSS) in PC is an attractive option if it can provide satisfactory cancer control equivocal or near equivocal to standard techniques. In fact, these techniques are meeting increasing acceptance. Accordingly, the need to evaluate these options objectively is crucial in a comprehensive review is timely in order to increase awareness of these procedures, their applicability and provide guidance to the practicing urologist...
March 9, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28276159/comparative-proteome-analysis-of-wild-type-and-klotho-knockout-mouse-kidneys-using-a-combination-of-maldi-ims-and-lc-ms-ms
#12
Yoko Fujino, Tomoko Minamizaki, Ikue Hayashi, Asako Kawakami, Takaaki Miyaji, Kaoru Sakurai, Hirotaka Yoshioka, Katsuyuki Kozai, Mitsugi Okada, Yuji Yoshiko
PURPOSE: Mutation of the klotho gene in mice elicits a syndrome resembling accelerated human aging. However, there is limited evidence for the role of Klotho in the kidney. We conducted a comparative proteome analysis of wild-type (WT) and Klotho-knockout (kl(-/-) ) mouse kidneys to identify proteins involved in Klotho deficiency. EXPERIMENTAL DESIGN: MALDI-IMS of frozen kidney sections from 7-week-old male WT and kl(-/-) mice was used to determine genotype-specific differences in the MS distribution...
March 8, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28274481/no-detection-of-varicella-zoster-virus-in-temporal-arteries-of-patients-with-giant-cell-arteritis
#13
Francesco Muratore, Stefania Croci, Ione Tamagnini, Alessandro Zerbini, Salvatore Bellafiore, Lucia Belloni, Luigi Boiardi, Alessandra Bisagni, Nicolò Pipitone, Maria Parmeggiani, Alberto Cavazza, Carlo Salvarani
OBJECTIVE: Data on the presence of varicella-zoster virus (VZV) in temporal arteries of patients with giant cell arteritis (GCA) are controversial. We analyzed VZV infection in temporal arteries from Italian patients with temporal artery biopsy (TAB)-positive GCA, TAB-negative GCA, and controls. METHODS: A total of 79 formalin-fixed, paraffin-embedded (FFPE) TABs performed between 2009 and 2012 at a single institution from 34 TAB-positive GCA patients, 15 TAB-negative GCA patients, and 30 controls were retrieved...
February 10, 2017: Seminars in Arthritis and Rheumatism
https://www.readbyqxmd.com/read/28273552/surgeon-performed-continuous-intraoperative-ultrasound-guidance-decreases-re-excisions-and-mastectomy-rates-in-breast-cancer
#14
Guldeniz Karadeniz Cakmak, Ali U Emre, Oge Tascilar, Burak Bahadir, Selcuk Ozkan
BACKGROUND: Intraoperative ultrasound guided (IUG) breast conserving surgery (BCS) is being increasingly embraced by breast surgeons worldwide. We aimed to compare the efficacy of IUG-BCS for palpable and nonpalpable breast cancer with respect to margin status, re-excision rate, tissue sacrifice and cost-time analysis. METHODS: Intraoperative localization protocol includes intraoperative ultrasound prior to excision to localize the lesion and guide the initial resection...
March 2, 2017: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/28272084/toluidine-blue-supports-differentiation-of-folliculocentric-basaloid-proliferation-from-basal-cell-carcinoma-on-frozen-sections-in-a-small-single-practice-cohort
#15
Matthew R Donaldson, L Arthur Weber
No abstract text is available yet for this article.
March 7, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28265757/one-stop-clinical-assessment-of-risk-for-endometrial-hyperplasia-oscar-endo-a-fast-track-protocol-for-evaluating-endometrial-pathologies
#16
Katrin Hefler-Frischmuth, Elisabeth Hirtl-Goergl, Verena Unterrichter, Judith Lafleur, Gudrun Brunnmayr-Petkin, Farid Moinfar, Lukas Hefler
PURPOSE: To evaluate a one-stop clinical assessment of risk for assessing endometrial pathologies (OSCAR-Endo), consisting of a fast-track protocol with hysteroscopy, dilation and curettage (D&C) with intraoperative frozen section analysis of the removed tissue in cases of hysteroscopic suspicion of malignancy. METHODS: In this prospective clinical trial, a total of 304 consecutive women with sonographically suspected endometrial hyperplasia and/or postmenopausal bleeding, underwent D&C with intraoperative frozen section analysis between May 2013 and September 2015...
March 6, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28260448/pathology-results-at-autopsy-in-brain-dead-patients-with-brain-tumors
#17
Farahnaz Sadegh Beigee, Shagin Shahryari, Meysam Mojtabaee, Mihan Pourabdollah Toutkaboni
OBJECTIVES: Brain tumors are the most challenging causes of brain deaths due to the lack of pathology results in many cases. It is not uncommon to find a brain tumor in a brain-dead patient with no pathology results or neuroradiology reports available; this would exclude the deceased from organ donation. The mortality that occurs while patients are on transplant wait lists motivated us to find a solution to prevent losing brain-dead patients as potential donors. We present our experiences in autopsy examinations of brain tumors and the results of frozen-section pathology...
February 2017: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/28259805/functional-visual-sensitivity-to-ultraviolet-wavelengths-in-the-pileated-woodpecker-dryocopus-pileatus-and-its-influence-on-foraging-substrate-selection
#18
Sean T O'Daniels, Dylan C Kesler, Jeanne D Mihail, Elisabeth B Webb, Scott J Werner
Most diurnal birds are presumed visually sensitive to near ultraviolet (UV) wavelengths, however, controlled behavioral studies investigating UV sensitivity remain few. Although woodpeckers are important as primary cavity excavators and nuisance animals, published work on their visual systems is limited. We developed a novel foraging-based behavioral assay designed to test UV sensitivity in the Pileated Woodpecker (Dryocopus pileatus). We acclimated 21 wild-caught woodpeckers to foraging for frozen mealworms within 1...
March 2, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28255734/robot-assisted-laparoscopic-transperitoneal-infrarenal-lymphadenectomy-in-patients-with-locally-advanced-cervical-cancer-by-single-docking-do-we-need-a-backup-procedure
#19
Fatih Gucer, Selim Misirlioglu, Nuri Ceydeli, Cagatay Taskiran
To present our initial experience on the feasibility of robotic transperitoneal para-aortic lymphadenectomy up to left renal vein via single docking approach by high port insertion technique followed by left shoulder docking as a rescue backup procedure in surgically obstructed patients undergoing surgical staging because of locally advanced cervical cancer (LACC). Prospective observational preliminary study. Canadian Task Force classification II-3. Tertiary-care academic affiliated private hospital. Ten patients with LACC who underwent robotic transperitoneal infrarenal para-aortic lymphadenectomy between January 2012 and December 2014...
March 2, 2017: Journal of Robotic Surgery
https://www.readbyqxmd.com/read/28255674/testing-of-viable-human-skin-cell-dilution-cultures-as-an-approach-to-validating-microsampling
#20
Stephen A Ainger, X L Hilary Yong, H Peter Soyer, Richard A Sturm
Skin biopsies are a valuable technique in the diagnosis of cutaneous inflammatory and neoplastic conditions. We were interested to test the minimal size or equivalent volume by dilution of proteolytically disassociated skin tissue required to allow the isolation and propagation of cutaneous cells, for freezing, storage and biochemical analysis. It was possible to propagate with 100% efficiency fibroblast and melanocytic cells from a 0.1 to 0.5 mm(3) equivalent neonatal foreskin sample using a combination of DispaseII and CollagenaseIV...
March 3, 2017: Archives of Dermatological Research
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