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https://www.readbyqxmd.com/read/28930488/the-impact-of-repeated-freeze-thaw-cycles-on-the-quality-of-biomolecules-in-four-different-tissues
#1
Xiaoli Ji, Min Wang, Lingling Li, Fang Chen, Yanyang Zhang, Qian Li, Junmei Zhou
High-quality biosamples are valuable resources for biomedical research. However, some tissues are stored without being sectioned into small aliquots and have to undergo repeated freeze-thaw cycles throughout prolonged experimentation. Little is known regarding the effects of repeated freeze-thaw cycles on the quality of biomolecules in tissues. The aim of this study was to evaluate the impact of repeated freeze-thaw (at room temperature or on ice) cycles on biomolecules and gene expression in four different types of tissues...
September 20, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28929985/characterizing-trends-in-fruit-and-vegetable-intake-in-the-usa-by-self-report-and-by-supply-and-disappearance-data-2001-2014
#2
Zach Conrad, Kenneth Chui, Lisa Jahns, Christian J Peters, Timothy S Griffin
OBJECTIVE: To examine the comparability of fruit and vegetable (F&V) intake data in the USA from 2001 to 2014 between data acquired from two national data collection programmes. DESIGN: Cross-sectional analysis. Linear regression models estimated trends in daily per capita intake of total F&V. Pooled differences in intake of individual F&V (n 109) were examined by processing form (fresh, frozen, canned, dried and juice). SETTING: What We Eat in America (WWEIA, 2001-2014) and Loss-Adjusted Food Availability data series (LAFA, 2001-2014)...
September 20, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28929330/development-of-a-rapid-analysis-method-for-bone-resection-margins-for-oral-squamous-cell-carcinoma-by-immunoblotting
#3
Claudia Haase, Bernd Lethaus, Ruth Knüchel-Clarke, Frank Hölzle, Angela Cassataro, Till Braunschweig
The purpose of this proof-of-principle study was to develop a rapid and approachable method to analyse bone resection margins in patients with oral squamous cell carcinoma (OSCC) in an intraoperative setting, similar to assessing frozen sections of soft tissue. Bone excision and risk of remaining tumour cells could be minimised, thus improving reconstruction measures and facilitating convalescence. Frozen, sawed wafers of porcine bone artificially combined with porcine skin (simulating OSCC properties) were used to develop and evaluate a new molecular method: protein transfer from non-decalcified, sawed wafers onto a membrane stained by immunofluorescence (Tissue-ProtTrans)...
September 19, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28928676/effects-of-immune-activation-during-early-or-late-gestation-on-n-methyl-d-aspartate-receptor-measures-in-adult-rat-offspring
#4
Tasnim Rahman, Katerina Zavitsanou, Tertia Purves-Tyson, Lauren R Harms, Crystal Meehan, Ulrich Schall, Juanita Todd, Deborah M Hodgson, Patricia T Michie, Cyndi Shannon Weickert
BACKGROUND: Glutamatergic receptor [N-methyl-d-aspartate receptor (NMDAR)] alterations within cortex, hippocampus, and striatum are linked to schizophrenia pathology. Maternal immune activation (MIA) is an environmental risk factor for the development of schizophrenia in offspring. In rodents, gestational timing of MIA may result in distinct behavioral outcomes in adulthood, but how timing of MIA may impact the nature and extent of NMDAR-related changes in brain is not known. We hypothesize that NMDAR-related molecular changes in rat cortex, striatum, and hippocampus are induced by MIA and are dependent on the timing of gestational inflammation and sex of the offspring...
2017: Frontiers in Psychiatry
https://www.readbyqxmd.com/read/28927729/contrast-enhanced-ultrasound-for-diagnosis-of-an-enlarged-cervical-lymph-node-in-a-patient-with-oropharyngeal-cancer-a-case-report
#5
Shalva R Gvetadze, Ping Xiong, Jiang Li, Mingming Lv, Jun Li, Xin Yang, Konstantin D Ilkaev, Jian Sun
In oral and oropharyngeal cancer, the presence of regional neck metastasis strongly influences treatment planning and survival prognosis. A number of imaging techniques can be utilized in the clinic for diagnosis and staging. A patient with oropharyngeal cancer was staged T2 cN1 after clinical examination, computed tomography, and (18)F-fluorodeoxyglucose positron emission tomography with computed tomography. Contrast-enhanced microbubble imaging was applied for diagnosis of a lymph node suspected of harboring a metastasis...
August 24, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28924667/correlative-in-resin-super-resolution-fluorescence-and-electron-microscopy-of-cultured-cells
#6
Errin Johnson, Rainer Kaufmann
Correlative super-resolution light and electron microscopy (super-resolution CLEM) is a powerful and emerging tool in biological research. The practical realization of these two very different microscopy techniques with their individual requirements remains a challenging task. There is a broad range of approaches to choose from, each with their own advantages and limitations. Here, we present a detailed protocol for in-resin super-resolution CLEM of high-pressure frozen and freeze substituted cultured cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28916856/a-faster-and-simpler-way-of-operation-for-meckel-s-diverticulum-basal-ligation-combined-with-intraoperative-frozen-section
#7
Jun Lei, Wei Xu, Wenping Yang, Juhua Xiao, Hui Huang, QingQiang Deng, Hongyan Xu, Liang Feng, Qiang Tao, Shouhua Zhang
BACKGROUND: The key step in Meckel's diverticulectomy (MD) is to achieve complete resection of MD along with the ectopic epithelium. Currently main treatment methods for Meckel's diverticulum are either intestinal resection and anastomosis or wedge resection. Here we introduced a new method to treat MD. The goal of this study was to investigate the clinical effects and advantages of a new operation method for Meckel's diverticulum: basal ligation combined with intraoperative frozen section...
September 15, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28916508/development-of-a-whole-slide-imaging-system-on-smartphones-and-evaluation-with-frozen-section-samples
#8
Hong Yu, Feng Gao, Liren Jiang, Shuoxin Ma
BACKGROUND: The aim was to develop scalable Whole Slide Imaging (sWSI), a WSI system based on mainstream smartphones coupled with regular optical microscopes. This ultra-low-cost solution should offer diagnostic-ready imaging quality on par with standalone scanners, supporting both oil and dry objective lenses of different magnifications, and reasonably high throughput. These performance metrics should be evaluated by expert pathologists and match those of high-end scanners. OBJECTIVE: The aim was to develop scalable Whole Slide Imaging (sWSI), a whole slide imaging system based on smartphones coupled with optical microscopes...
September 15, 2017: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/28915974/rapid-and-accurate-intraoperative-pathological-diagnosis-by-artificial-intelligence-with-deep-learning-technology
#9
Jing Zhang, Yanlin Song, Fan Xia, Chenjing Zhu, Yingying Zhang, Wenpeng Song, Jianguo Xu, Xuelei Ma
Frozen section is widely used for intraoperative pathological diagnosis (IOPD), which is essential for intraoperative decision making. However, frozen section suffers from some drawbacks, such as time consuming and high misdiagnosis rate. Recently, artificial intelligence (AI) with deep learning technology has shown bright future in medicine. We hypothesize that AI with deep learning technology could help IOPD, with a computer trained by a dataset of intraoperative lesion images. Evidences supporting our hypothesis included the successful use of AI with deep learning technology in diagnosing skin cancer, and the developed method of deep-learning algorithm...
September 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28915147/measuring-thrombin-activity-in-frozen-brain-tissue
#10
Gilad Reuveni, Valery Golderman, Efrat Shavit-Stein, Yossi Rosman, Shai Shrot, Joab Chapman, Sagi Harnof
Thrombin is a coagulation factor implicated in various pathological and physiological processes in the brain, exerting beneficial and deleterious effects in a concentration-dependent manner. Measurement of thrombin activity levels in pathological animal models is needed and in some cases, because of technical considerations, only frozen samples are available. In the current study, we used a quantitative method to evaluate thrombin activity in fresh and frozen brain sections of 43 male and female adult healthy mice...
September 14, 2017: Neuroreport
https://www.readbyqxmd.com/read/28913301/concordant-surgical-treatment-non-melanocytic-skin-cancer-of-the-head-and-neck
#11
Wan Cheol Ryu, In Chang Koh, Yong Hae Lee, Jong Hyun Cha, Sang Il Kim, Chang Gyun Kim
BACKGROUND: Skin cancer is the most common type of cancer. Of the 4 million skin lesions excised annually worldwide, approximately 2 million are considered cancerous. In this study, we aimed to describe a regional experience with skin cancers treated by a single senior surgeon and to provide a treatment algorithm. METHODS: The medical records of 176 patients with head and neck non-melanocytic skin cancer (NMSC) who were treated by a single surgeon at our institution between January 2010 and May 2016 were retrospectively reviewed, and their data (age, sex, pathological type, tumor location/size, treatment modality) were analyzed...
March 2017: Arch Craniofac Surg
https://www.readbyqxmd.com/read/28912417/comparison-of-leukocyte-esterase-testing-of-synovial-fluid-with-synovial-histology-for-the-diagnosis-of-periprosthetic-joint-infection
#12
Rui Li, Xiang Li, Baozhan Yu, Xin Li, Xinggui Song, Heng Li, Chi Xu, Jiying Chen
BACKGROUND Periprosthetic joint infection (PJI) is a complication of total joint arthroplasty (TJA). The leukocyte esterase (LE) strip test and histology are diagnostic methods for PJI. The aims of this study were to determine the sensitivity and specificity of the LE strip test and to compare it with histology in the diagnosis of PJI. MATERIAL AND METHODS Between January and December 2015, 93 patients who underwent TJA with PJI were enrolled in the study. Synovial fluid samples were tested with an LE strip, and three synovial tissue samples from each patient underwent frozen section and formalin-fixed histology...
September 15, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28912407/diagnostic-efficacy-of-percutaneous-renal-tumor-biopsy-concomitant-use-of-frozen-section-to-accurately-diagnose-renal-tumor-with-necrosis
#13
Junichi Hori, Shin Kobayashi, Gaku Tamaki, Makoto Azumi, Hidehiro Kakizaki
OBJECTIVES: To evaluate the diagnostic efficacy of percutaneous renal tumor biopsy. METHODS: We retrospectively investigated 23 patients who underwent percutaneous renal tumor biopsy since 2008 at Department of Renal and Urologic Surgery, Asahikawa Medical University Hospital. We examined indications of biopsy, diagnostic concordance rate between urologists, radiologists and biopsy findings, pathological findings and biopsy-related complications. RESULTS: Renal tumor biopsy was performed under ultrasonography guidance in 21 patients and computed tomography guidance in 2...
September 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/28911827/single-site-robot-assisted-laparoscopic-staging-surgery-for-presumed-clinically-early-stage-ovarian-cancer
#14
Ji Geun Yoo, Woo Jin Kim, Keun Ho Lee
STUDY OBJECTIVE: To present the demonstration of Robotic-assisted laparoendoscopic single-site(R-LESS) staging surgery in presumed clinically early stage ovarian cancer. DESIGN: A step-by-step presentation of the procedure using video (Canadian Task Force classification III). SETTING: A university hospital. INTERVENTION: A 29-year-old woman was referred from a local clinic for an 8x6cm sized left ovarian tumor suggesting malignancy...
September 11, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28910098/fast-and-simple-protocols-for-mass-spectrometry-based-proteomics-of-small-fresh-frozen-uterine-tissue-sections
#15
Irena Dapic, Naomi Uwugiaren, Petra J Jansen, Garry L Corthals
Human tissues are an important link between organ specific spatial molecular information, patient pathology and patient treatment options. However, patient tissues are uniquely obtained by time and location, and limited in their availability and size. Currently little knowledge exists about appropriate and simplified protocols for routine MS-based analysis of the various types and sizes of tissues. Following standard procedures used in pathology we selected small fresh frozen uterine tissue samples to investigate how the tissue preparation protocol affected the subsequent proteomics analysis...
September 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28904927/cervical-lymph-nodes-harbinger-of-benign-inclusions-as-well-as-metastatic-deposits-of-thyroid-malignancy
#16
Samarth Shukla, Sourya Acharya
Lymph nodes can be harbinger to benign epithelial inclusions as well as metastatic deposits. Cervical lymph nodes are home to benign epithelial inclusions from thyroid better known as lateral aberrant thyroid as well as inclusions from salivary gland due to the unique embryologic origins of nodes with these organs. We present a case of a young female suspected of thyroid malignancy who was intraoperative diagnosed by frozen sections with papillary carcinoma of the thyroid along with bilateral cervical nodes (Level I-V) positive for reactive lymphadenopathy with Level II node being positive to benign salivary gland inclusions and Level VI node being positive to metastatic deposits of papillary carcinoma of the thyroid...
July 2017: International Journal of Applied and Basic Medical Research
https://www.readbyqxmd.com/read/28899632/the-deposition-of-anti-dna-igg-contributes-to-the-development-of-cutaneous-lupus-erythematosus
#17
Yingying Dong, Yi Zhang, Linlin Xia, Ping Wang, Jingyun Chen, Meifeng Xu, Xingyin Liu, Yumin Xia
Anti-DNA IgG is a hallmark of systemic lupus erythematosus and induces internal injuries in patients. It is known that cutaneous lupus erythematosus (CLE) involves the deposition of autoantibodies in the dermoepidermal junction of the skin and that anti-DNA IgG binds specifically to keratinocytes. However, the definite role of anti-DNA IgG in CLE remains unclear. The purpose of this study was to elucidate the effect of anti-DNA IgG on keratinocytes in CLE. Skin tissues were collected from patients with CLE and healthy controls...
September 9, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28894930/oncological-safety-and-technical-feasibility-of-nipple-sparing-mastectomy-for-breast-cancer-the-hong-kong-experience
#18
Yolanda Ho-Yan Chan, Wai-Ming Yau, Polly Suk-Yee Cheung
BACKGROUND: Nipple-sparing mastectomy (NSM) has gained widespread popularity in recent years. Nonetheless, patient selection, technical consideration and oncological safety of its extension to breast cancer treatment remain uncertain. Few publications have reviewed the application of NSM in Asian populations. METHODS: We retrospectively reviewed 91 women with malignant breast tumours, who underwent 97 NSM in Hong Kong Sanatorium and Hospital from 2009 to 2015. Breast cancer patients who required mastectomy and opted for immediate reconstruction were considered for NSM if they showed no obvious nipple involvement clinically...
September 11, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28892665/difference-in-glycogen-metabolism-glycogen-synthesis-and-glycolysis-between-normal-and-dysplastic-malignant-oral-epithelium
#19
Hitoshi Aizawa, Shin-Ichi Yamada, Tiepeng Xiao, Tetsu Shimane, Kiyonori Hayashi, Fangfang Qi, Hirokazu Tanaka, Hiroshi Kurita
BACKGROUND: The purpose of this study was to investigate a difference in glycogen metabolism (glycogen synthesis and glycolysis) between the iodine stained (normal non-keartinized) and the unstained (dysplasctic/malignant) oral epithelium. METHODS: Twenty-one frozen tissue samples of iodine-stained and unstained mucosal tissue were obtained from 21 OSCC patients. Serial frozen sections were cut and examined with the hematoxylin-eosin and periodic acid-Schiff methods and immunohistochemical (IHC) staining for Ki67, P53, molecules associated with glycogenesis (i...
September 2, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28892131/use-of-endoscopy-to-determine-the-resection-margin-during-laparoscopic-gastrectomy-for-cancer
#20
S Kawakatsu, M Ohashi, N Hiki, S Nunobe, M Nagino, T Sano
BACKGROUND: It can be difficult to determine the transection line during totally laparoscopic surgery for early gastric cancer owing to lack of tactile feedback. This retrospective cohort study aimed to assess the role of intraoperative endoscopy in determining the resection margin in totally laparoscopic gastrectomy. METHODS: Consecutive patients with histologically confirmed gastric cancer who underwent laparoscopic gastrectomy between March 2012 and July 2015 were eligible...
September 11, 2017: British Journal of Surgery
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