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https://www.readbyqxmd.com/read/29691678/the-determination-of-ochratoxin-a-based-on-the-electrochemical-aptasensor-by-carbon-aerogels-and-methylene-blue-assisted-signal-amplification
#1
Min Wei, Wenyang Zhang
In this work, a novel aptamer-based electrochemical biosensor was developed for the determination of Ochratoxin A (OTA) by using carbon aerogels (CAs) and methylene blue (MB) as signal amplification strategy. CAs was used as carrier to load the abundant of complementary DNA (cDNA), which could enhance the hybridization between CAs-cDNA and aptamer immobilized on the electrode surface, thus provide more double-stranded DNA for MB intercalation. The current of MB on the CAs-cDNA/apt/AuE sensor was twice that on the cDNA/apt/AuE sensor, which indicated that the CAs with high surface area enabled a higher loading of the cDNA and absorbed more MB, thus realized the signal amplification strategy...
April 25, 2018: Chemistry Central Journal
https://www.readbyqxmd.com/read/29691341/epigenetic-regulation-of-tumor-suppressors-by-helicobacter-pylori-enhances-ebv-induced-proliferation-of-gastric-epithelial-cells
#2
Saurabh Pandey, Hem Chandra Jha, Sanket Kumar Shukla, Meghan K Shirley, Erle S Robertson
Helicobacter pylori and Epstein-Barr virus (EBV) are two well-known contributors to cancer and can establish lifelong persistent infection in the host. This leads to chronic inflammation, which also contributes to development of cancer. Association with H. pylori increases the risk of gastric carcinoma, and coexistence with EBV enhances proliferation of infected cells. Further, H. pylori -EBV coinfection causes chronic inflammation in pediatric patients. We have established an H. pylori -EBV coinfection model system using human gastric epithelial cells...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29691193/cloning-and-high-level-expression-of-thermus-thermophilus-reca-in-e-coli-purification-and-novel-use-in-hbv-diagnostics
#3
Sudarson Sundarrajan, Sneha Rao, Sriram Padmanabhan
We studied the role of Thermus thermophilus Recombinase A (RecA) in enhancing the PCR signals of DNA viruses such as Hepatitis B virus (HBV). The RecA gene of a thermophilic eubacterial strain, T. thermophilus, was cloned and hyperexpressed in Escherichia coli. The recombinant RecA protein was purified using a single heat treatment step without the use of any chromatography steps, and the purified protein (>95%) was found to be active. The purified RecA could enhance the polymerase chain reaction (PCR) signals of HBV and improve the detection limit of the HBV diagnosis by real time PCR...
April 12, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29690904/ocean-c-mapping-hubs-of-open-chromatin-interactions-across-the-genome-reveals-gene-regulatory-networks
#4
Tingting Li, Lumeng Jia, Yong Cao, Qing Chen, Cheng Li
We develop a method called open chromatin enrichment and network Hi-C (OCEAN-C) for antibody-independent mapping of global open chromatin interactions. By integrating FAIRE-seq and Hi-C, OCEAN-C detects open chromatin interactions enriched by active cis-regulatory elements. We identify more than 10,000 hubs of open chromatin interactions (HOCIs) in human cells, which are mainly active promoters and enhancers bound by many DNA-binding proteins and form interaction networks crucial for gene transcription. In addition to identifying large-scale topological structures, including topologically associated domains and A/B compartments, OCEAN-C can detect HOCI-mediated chromatin interactions that are strongly associated with gene expression, super-enhancers, and broad H3K4me3 domains...
April 24, 2018: Genome Biology
https://www.readbyqxmd.com/read/29690620/silk-route-to-the-acceptance-and-re-implementation-of-bacteriophage-therapy-part-ii
#5
Wilbert Sybesma, Christine Rohde, Pavol Bardy, Jean-Paul Pirnay, Ian Cooper, Jonathan Caplin, Nina Chanishvili, Aidan Coffey, Daniel De Vos, Amber Hartman Scholz, Shawna McCallin, Hilke Marie Püschner, Roman Pantucek, Rustam Aminov, Jiří Doškař, D İpek Kurtbӧke
This perspective paper follows up on earlier communications on bacteriophage therapy that we wrote as a multidisciplinary and intercontinental expert-panel when we first met at a bacteriophage conference hosted by the Eliava Institute in Tbilisi, Georgia in 2015. In the context of a society that is confronted with an ever-increasing number of antibiotic-resistant bacteria, we build on the previously made recommendations and specifically address how the Nagoya Protocol might impact the further development of bacteriophage therapy...
April 23, 2018: Antibiotics
https://www.readbyqxmd.com/read/29690600/microplate-chemiluminescent-assay-for-dna-detection-using-apoperoxidase-oligonucleotide-as-capture-conjugate-and-hrp-streptavidin-signaling-system
#6
Ivan Sakharov
A covalent conjugate of horseradish apoperoxidase and amino-containing oligonucleotide was synthesized for the first time. Using the obtained conjugate as a capture reagent chemiluminescent microtiter plate-based assay for detection of 35-mer fragment of hepatitis B virus (HBV) DNA (proof-of-concept analyte) was developed. To detect the target DNA, a signaling system consisted of biotinylated reporter oligonucleotide and HRP-streptavidin conjugate was used. The high sensitivity of the assay was due to the enhanced chemiluminescence reaction, where 3-(10′-phenothiazinyl)propane-1-sulfonate/ N -morpholinopyridine pair was used as an enhancer...
April 23, 2018: Sensors
https://www.readbyqxmd.com/read/29690543/memory-function-in-feeding-habit-transformation-of-mandarin-fish-siniperca-chuatsi
#7
Yaqi Dou, Shan He, Xu-Fang Liang, Wenjing Cai, Jie Wang, Linjie Shi, Jiao Li
Mandarin fish refuse dead prey fish or artificial diets and can be trained to transform their inborn feeding habit. To investigate the effect of memory on feeding habit transformation, we compared the reaction time to dead prey fish and the success rate of feeding habit transformation to dead prey fish with training of mandarin fish in the 1st experimental group (trained once) and the 2nd experimental group (trained twice). The mandarin fish in the 2nd group had higher success rate of feeding habit transformation (100%) than those in the 1st group (67%), and shorter reaction time to dead prey fish (<1 s) than those in the 1st group (>1 s)...
April 22, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29690513/research-progress-on-rolling-circle-amplification-rca-based-biomedical-sensing
#8
REVIEW
Lide Gu, Wanli Yan, Le Liu, Shujun Wang, Xu Zhang, Mingsheng Lyu
Enhancing the limit of detection (LOD) is significant for crucial diseases. Cancer development could take more than 10 years, from one mutant cell to a visible tumor. Early diagnosis facilitates more effective treatment and leads to higher survival rate for cancer patients. Rolling circle amplification (RCA) is a simple and efficient isothermal enzymatic process that utilizes nuclease to generate long single stranded DNA (ssDNA) or RNA. The functional nucleic acid unit (aptamer, DNAzyme) could be replicated hundreds of times in a short period, and a lower LOD could be achieved if those units are combined with an enzymatic reaction, Surface Plasmon Resonance, electrochemical, or fluorescence detection, and other different kinds of biosensor...
April 21, 2018: Pharmaceuticals
https://www.readbyqxmd.com/read/29689377/ambient-particulate-matter-activates-the-aryl-hydrocarbon-receptor-in-dendritic-cells-and-enhances-th17-polarization
#9
Alejandro R Castañeda, Kent E Pinkerton, Keith J Bein, Alfonso Magaña-Méndez, Houa T Yang, Paul Ashwood, Christoph F A Vogel
The objective of this study was to explore the role of the aryl hydrocarbon receptor (AhR) in ambient particulate matter (PM)-mediated activation of dendritic cells (DCs) and Th17-immune responses in vitro. To assess the potential role of the AhR in PM-mediated activation of DCs, co-stimulation, and cytokine expression, bone marrow (BM)-derived macrophages and DCs from C57BL6 wildtype or AhR knockout (AhR-/- ) mice were treated with PM. Th17 differentiation was assessed via co-cultures of wildtype or AhR-/- BMDCs with autologous naive T cells...
April 21, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29689351/the-fruits-of-gleditsia-sinensis-lam-inhibits-adipogenesis-through-modulation-of-mitotic-clonal-expansion-and-stat3-activation-in-3t3-l1-cells
#10
Ji-Hye Lee, Younghoon Go, Bonggi Lee, Youn-Hwan Hwang, Kwang Il Park, Won-Kyung Cho, Jin Yeul Ma
ETHNOPHARMACOLOGICAL RELEVANCE: Gleditsia sinensis Lam. (G. sinensis) has been used in Oriental medicine for tumor, thrombosis, inflammation-related disease, and obesity. AIM OF THE STUDY: The pharmacological inhibitory effects of fruits of G. sinensis (GFE) on hyperlipidemia have been reported, but its inhibitory effects on adipogenesis and underlying mechanisms have not been elucidated. Herein we evaluated the anti-adipogenic effects of GFE and described the underlying mechanisms...
April 21, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29689167/tailoring-the-supramolecular-structure-of-guanidinylated-pullulan-toward-enhanced-genetic-photodynamic-therapy
#11
Jie Zhou, Aisha Roshan Mohamed Wali, Shengnan Ma, Yiyan He, Dong Yue, James Zhenggui Tang, Zhongwei Gu
In the progress of designing a gene carrier system, what is urgently needed is a balance of excellent safety and satisfactory efficiency. Herein, a straightforward and versatile synthesis of a cationic guanidine-decorated dendronized pullulan (OGG3P) for efficient genetic photodynamic therapy was proposed. OGG3P was able to block the mobility of DNA from a weight ratio of 2. However, G3P lacking guanidine residues could not block DNA migration until at a weight ratio of 15, revealing guanidination could facilitate DNA condensation via specific guanidinium-phosphate interactions...
April 24, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29688543/natural-selection-and-origin-of-a-melanistic-allele-in-north-american-gray-wolves
#12
Rena M Schweizer, Arun Durvasula, Joel Smith, Samuel H Vohr, Daniel R Stahler, Marco Galaverni, Olaf Thalmann, Douglas W Smith, Ettore Randi, Elaine A Ostrander, Richard E Green, Kirk E Lohmueller, John Novembre, Robert K Wayne
Pigmentation is often used to understand how natural selection affects genetic variation in wild populations since it can have a simple genetic basis, and can affect a variety of fitness-related traits (e.g., camouflage, thermoregulation, and sexual display). In gray wolves, the K locus, a β-defensin gene, causes black coat color via a dominantly inherited KB allele. The allele is derived from dog-wolf hybridization and is at high frequency in North American wolf populations. We designed a DNA capture array to probe the geographic origin, age, and number of introgression events of the KB allele in a panel of 331 wolves and 20 dogs...
May 1, 2018: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29687555/smart-dna-hydrogels-integrated-nanochannels-with-high-ion-flux-and-adjustable-selective-ionic-transport
#13
Lei Jiang, Yafeng Wu, Dianyu Wang, Itamar Willner, Ye Tian
Here we present nanochannels based on smart DNA hydrogels as stimulus-responsive architecture for the first time. Different from other responsive molecules existed in the nanochannel in monolayer configurations, the DNA hydrogels are three dimensional networks with space negative charges, the ion flux and rectification ratio are significantly enhanced. Upon cyclic treatment with K+ ions and crown ether, the DNA hydrogel states could be reversibly switched between less stiff and stiff networks, providing the gating mechanism of the nanochannel...
April 24, 2018: Angewandte Chemie
https://www.readbyqxmd.com/read/29687366/molecular-modeling-of-non-covalent-binding-of-ligustrum-lucidum-secoiridoid-glucosides-to-ap-1-matrix-metalloproteinase-pathway-components
#14
Pathomwat Wongrattanakamon, Piyarat Nimmanpipug, Busaban Sirithunyalug, Wantida Chaiyana, Supat Jiranusornkul
Ligustrum lucidum secoiridoid glucosides have been demonstrated to treat various types of diseases such as inflammation, pain, hepatotoxicity and hyperlipidermic as well as tonic for liver and kidney. Matrix metalloproteinases (MMPs) play a key role upon the pathology of photoaging. The present computational study showed that among the six secoiridoid glucosides (ligustroside, lucidumoside A, lucidumoside C, neonuezhenide, oleoside dimethylester, and oleuropein), ligustroside and lucidumoside A competitively inhibit all MMP-1, MMP-3, and MMP-9 activities in the docking models...
April 23, 2018: Journal of Bioenergetics and Biomembranes
https://www.readbyqxmd.com/read/29687271/biological-and-nanotechnological-applications-using-interactions-between-ionic-liquids-and-nucleic-acids
#15
REVIEW
Hisae Tateishi-Karimata, Naoki Sugimoto
Nucleic acids have emerged as powerful biological and nanotechnological tools. In biological and nanotechnological experiments, methods of extracting and purifying nucleic acids from various types of cells and their storage are critical for obtaining reproducible experimental results. In nanotechnological experiments, methods for regulating the conformational polymorphism of nucleic acids and increasing sequence selectivity for base pairing of nucleic acids are important for developing nucleic acid-based nanomaterials...
April 23, 2018: Biophysical Reviews
https://www.readbyqxmd.com/read/29687201/folate-promotes-s-adenosyl-methionine-reactions-and-the-microbial-methylation-cycle-and-boosts-ruminants-production-and-reproduction
#16
REVIEW
Imtiaz Hussain Raja Abbasi, Farzana Abbasi, Lamei Wang, Mohamed E Abd El Hack, Ayman A Swelum, Ren Hao, Junhu Yao, Yangchun Cao
Folate has gained significant attention due to its vital role in biological methylation and epigenetic machinery. Folate, or vitamin (B9 ), is only produced through a de novo mechanism by plants and micro-organisms in the rumen of mature animals. Although limited research has been conducted on folate in ruminants, it has been noted that ruminal synthesis could not maintain folate levels in high yielding dairy animals. Folate has an essential role in one-carbon metabolism and is a strong antiproliferative agent...
April 23, 2018: AMB Express
https://www.readbyqxmd.com/read/29687126/highly-labeled-methylene-blue-ds-dna-silica-nanoparticles-for-signal-enhancement-of-immunoassays-application-to-the-sensitive-detection-of-bacteria-in-human-platelet-concentrates
#17
Romaric Bonnet, Carole Farre, Lionel Valera, Ludivine Vossier, Fanny Léon, Typhaine Dagland, Agnès Pouzet, Nicole Jaffrézic-Renault, Jeannette Fareh, Chantal Fournier-Wirth, Carole Chaix
A nanoparticle-based electrochemical sandwich immunoassay was developed for bacteria detection in platelet concentrates. For the assay, magnetic beads were functionalized with antibodies to allow the specific capture of bacteria from the complex matrix, and innovative methylene blue-DNA/nanoparticle assemblies provided the electrochemical response for amplified detection. This nanoparticular system was designed as a temperature-sensitive nano-tool for electrochemical detection. First, oligonucleotide-functionalized nanoparticles were obtained by direct synthesis of the DNA strands on the nanoparticle surface using an automated oligonucleotide synthesizer...
April 24, 2018: Analyst
https://www.readbyqxmd.com/read/29686974/sirt6-acts-as-a-negative-regulator-in-dengue-virus-induced-inflammatory-response-by-targeting-the-dna-binding-domain-of-nf-%C3%AE%C2%BAb-p65
#18
Pengcheng Li, Yufei Jin, Fei Qi, Fangyi Wu, Susu Luo, Yuanjiu Cheng, Ruth R Montgomery, Feng Qian
Dengue virus (DENV) is a mosquito-borne single-stranded RNA virus causing human disease with variable severity. The production of massive inflammatory cytokines in dengue patients has been associated with dengue disease severity. However, the regulation of these inflammatory responses remains unclear. In this study, we report that SIRT6 is a negative regulator of innate immune responses during DENV infection. Silencing of Sirt6 enhances DENV-induced proinflammatory cytokine and chemokine production. Overexpression of SIRT6 inhibits RIG-I-like receptor (RLR) and Toll-like receptor 3 (TLR3) mediated NF-κB activation...
2018: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29686887/role-of-dna-methylation-in-altered-gene-expression-patterns-in-adult-zebrafish-danio-rerio-exposed-to-3-3-4-4-5-pentachlorobiphenyl-pcb-126
#19
Neelakanteswar Aluru, Sibel I Karchner, Keegan S Krick, Wei Zhu, Jiang Liu
There is growing evidence that environmental toxicants can affect various physiological processes by altering DNA methylation patterns. However, very little is known about the impact of toxicant-induced DNA methylation changes on gene expression patterns. The objective of this study was to determine the genome-wide changes in DNA methylation concomitant with altered gene expression patterns in response to 3, 3', 4, 4', 5-pentachlorobiphenyl (PCB126) exposure. We used PCB126 as a model environmental chemical because the mechanism of action is well-characterized, involving activation of aryl hydrocarbon receptor, a ligand-activated transcription factor...
January 2018: Environmental Epigenetics
https://www.readbyqxmd.com/read/29686814/an-innovative-way-to-highlight-the-power-of-each-polymorphism-on-elite-athletes-phenotype-expression
#20
Valentina Contrò, Gabriella Schiera, Antonino Abbruzzo, Antonino Bianco, Alessandra Amato, Alessia Sacco, Alessandra Macchiarella, Antonio Palma, Patrizia Proia
The purpose of this study was to determine the probability of soccer players having the best genetic background that could increase performance, evaluating the polymorphism that are considered Performance Enhancing Polymorphism (PEPs) distributed on five genes: PPARα, PPARGC1A, NRF2, ACE e CKMM. Particularly, we investigated how each polymorphism works directly or through another polymorphism to distinguish elite athletes from non-athletic population. Sixty professional soccer players (age 22.5 ± 2.2) and sixty healthy volunteers (age 21...
January 12, 2018: European Journal of Translational Myology
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