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https://www.readbyqxmd.com/read/28929330/development-of-a-rapid-analysis-method-for-bone-resection-margins-for-oral-squamous-cell-carcinoma-by-immunoblotting
#1
Claudia Haase, Bernd Lethaus, Ruth Knüchel-Clarke, Frank Hölzle, Angela Cassataro, Till Braunschweig
The purpose of this proof-of-principle study was to develop a rapid and approachable method to analyse bone resection margins in patients with oral squamous cell carcinoma (OSCC) in an intraoperative setting, similar to assessing frozen sections of soft tissue. Bone excision and risk of remaining tumour cells could be minimised, thus improving reconstruction measures and facilitating convalescence. Frozen, sawed wafers of porcine bone artificially combined with porcine skin (simulating OSCC properties) were used to develop and evaluate a new molecular method: protein transfer from non-decalcified, sawed wafers onto a membrane stained by immunofluorescence (Tissue-ProtTrans)...
September 19, 2017: Head and Neck Pathology
https://www.readbyqxmd.com/read/28924572/stereovision-based-integrated-system-for-point-cloud-reconstruction-and-simulated-brain-shift-validation
#2
Xiaochen Yang, Logan W Clements, Ma Luo, Saramati Narasimhan, Reid C Thompson, Benoit M Dawant, Michael I Miga
Intraoperative soft tissue deformation, referred to as brain shift, compromises the application of current image-guided surgery navigation systems in neurosurgery. A computational model driven by sparse data has been proposed as a cost-effective method to compensate for cortical surface and volumetric displacements. We present a mock environment developed to acquire stereoimages from a tracked operating microscope and to reconstruct three-dimensional point clouds from these images. A reconstruction error of 1 mm is estimated by using a phantom with a known geometry and independently measured deformation extent...
July 2017: Journal of Medical Imaging
https://www.readbyqxmd.com/read/28906177/brachyspira-hyodysenteriae-detection-in-the-large-intestine-of-slaughtered-pigs
#3
Friederike Zeeh, Silvio De Luca, Pamela Nicholson, Niels Grützner, Christina Nathues, Vincent Perreten, Heiko Nathues
Detection of subclinical Brachyspira hyodysenteriae infection in pig herds using feces is challenging. However, the ability to detect the pathogen in intestinal samples of slaughtered pigs has not been investigated, to our knowledge. Therefore, we determined the detection of B. hyodysenteriae in the colon, cecum, and rectum from slaughtered pigs. We analyzed the correlation between detection rates and intestinal lesions, ingesta or fecal consistency, and time from sample collection until processing. A total of 400 ingesta-mucosal (colon, cecum) and 200 fecal (rectum) samples from 200 pigs originating from 20 different herds were bacteriologically examined using selective culture followed by Brachyspira spp...
September 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28900710/coupling-matrix-assisted-ionization-with-high-resolution-mass-spectrometry-and-electron-transfer-dissociation-to-characterize-intact-proteins-and-post-translational-modifications
#4
Bingming Chen, Christopher B Lietz, Lingjun Li
Matrix-assisted ionization (MAI) is a recently developed ionization technique that produces multiply charged ions on either electrospray ionization (ESI) or matrix-assisted laser desorption/ionization (MALDI) platform without the need of high voltage or laser ablation. In this study, MAI has been coupled to a high resolution accurate mass (HRAM) hybrid instrument, the Orbitrap Elite mass spectrometer, with electron transfer dissociation (ETD) module for fast peptide and intact protein characterization. The softness of MAI process preserves labile post-translational modifications (PTM) and allows fragmentation and localization by ETD...
September 12, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28890621/soft-tissue-applications-of-er-cr-ysgg-laser-in-pediatric-dentistry
#5
Gyanendra Kumar, Ferah Rehman, Vivek Chaturvedy
Soft tissue laser, such as diode and Nd:YAG lasers were initially used in soft tissue lesions because of its increased success rate. It was because of the fact that these lasers were well-absorbed by chromophores, such as hemoglobin and melanin which are found abundant in the oral mucosa. The introduction of erbium family in 1990 comprising the Er:YAG and Er,Cr:YSGG lasers made the hard tissue laser a boon for dentistry. Erbium, chromium-doped yttrium, scandium, gallium and garnet (Er,Cr:YSGG) was introduced in 1997 for the surgical needs of clinical dentistry in general practice...
April 2017: International Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/28889322/efficiency-of-soft-tissue-incision-with-a-novel-445-nm-semiconductor-laser
#6
Andreas Braun, Moritz Kettner, Michael Berthold, Johannes-Simon Wenzler, Paul Günther Baptist Heymann, Roland Frankenberger
Using a 445-nm semiconductor laser for tissue incision, an effective cut is expected due to the special absorption properties of blue laser light in soft tissues. The aim of the present study was the histological evaluation of tissue samples after incision with a 445-nm diode laser. Forty soft tissue specimens were obtained from pork oral mucosa and mounted on a motorized linear translation stage. The handpiece of a high-frequency surgery device, a 970-nm semiconductor laser, and a 445-nm semiconductor laser were connected to the slide, allowing a constant linear movement (2 mm/s) and the same distance of the working tip to the soft tissue's surface...
September 9, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28888300/heterotopic-ossification-and-hypertrophic-scars
#7
REVIEW
Shailesh Agarwal, Michael Sorkin, Benjamin Levi
Burns and trauma cause superficial and deep soft tissue wounds that cannot heal to the preinjury state. Healing requires cell proliferation and differentiation into the injured tissue type, laying down extracellular matrix, often as collagens. Heterotopic ossification causes severe pain, nonhealing wounds, and restricted range of motion. Treatment includes radiation therapy, nonsteroidal anti-inflammatory drugs, bisphosphonates, and possibly surgical excision and prophylactic measures. Hypertrophic scars, nonosseous lesions caused by excessive collagen deposition, are often painful, functionally limiting, and aesthetically displeasing...
October 2017: Clinics in Plastic Surgery
https://www.readbyqxmd.com/read/28887783/a-novel-integration-of-spectral-domain-optical-coherence-tomography-and-laser-ablation-system-for-precision-treatment
#8
Yingwei Fan, Boyu Zhang, Wei Chang, Xinran Zhang, Hongen Liao
PURPOSE: Complete resection of diseased lesions reduces the recurrence of cancer, making it critical for surgical treatment. However, precisely resecting residual tumors is a challenge during operation. A novel integrated spectral-domain optical-coherence-tomography (SD-OCT) and laser-ablation therapy system for soft-biological-tissue resection is proposed. This is a prototype optical integrated diagnosis and therapeutic system as well as an optical theranostics system. METHODS: We develop an optical theranostics system, which integrates SD-OCT, a laser-ablation unit, and an automatic scanning platform...
September 9, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28876014/tracking-the-restructuring-of-oxidized-silver-indium-nanoparticles-under-a-reducing-atmosphere-by-environmental-hrtem
#9
Julien Ramade, Cyril Langlois, Michel Pellarin, Laurent Piccolo, Marie-Ange Lebeault, Thierry Epicier, Mimoun Aouine, Emmanuel Cottancin
Multimetallic nano-alloys display a structure and consequently physicochemical properties evolving in a reactive environment. Following and understanding this evolution is therefore crucial for future applications in gas sensing and heterogeneous catalysis. In view hereof, the structural evolution of oxidized Ag25In75 bimetallic nanoparticles under varying H2 partial pressures (PH2) and substrate temperatures (Ts) has been investigated in real-time through environmental transmission microscopy (E-TEM) while maintaining the atomic resolution...
September 21, 2017: Nanoscale
https://www.readbyqxmd.com/read/28873437/three-dimensional-hard-and-soft-tissue-imaging-of-the-human-cochlea-by-scanning-laser-optical-tomography-slot
#10
Nadine Tinne, Georgios C Antonopoulos, Saleh Mohebbi, José Andrade, Lena Nolte, Heiko Meyer, Alexander Heisterkamp, Omid Majdani, Tammo Ripken
The present study focuses on the application of scanning laser optical tomography (SLOT) for visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique which allows for tomographic imaging of the internal structure of transparent specimens. Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises decalcification, dehydration as well as optical clearing of the cochlea samples in toto...
2017: PloS One
https://www.readbyqxmd.com/read/28869625/phase-composition-and-morphology-characteristics-of-ceria-stabilized-zirconia-powders-obtained-via-sol-gel-method-with-various-ph-conditions
#11
Damian S Nakonieczny, Zbigniew K Paszenda, Marcin Basiaga, Tomasz Radko, Sabina Drewniak, Jacek Podwórny, Wojciech Bogacz
PURPOSE: High purity, fine crystalline, degradation-free at low temperature powders have attracted special interest in CAD/CAM prosthetic dentistry full ceramic restorations. This study reports the preparation and characterisation of zirconia-ceria (0.9 ZrO2 0.1 CeO2) powders. Materials were obtained from zirconium-n-alkoxide and cerium nitrate hexahydrate in the pH 2-4 and 8-10. METHODS: Zirconia- ceria powders were obtained with the sol-gel method in a humid-free environment...
2017: Acta of Bioengineering and Biomechanics
https://www.readbyqxmd.com/read/28867525/a-customised-digitally-engineered-solution-for-fixed-dental-rehabilitation-in-severe-bone-deficiency-a-new-innovative-line-extension-in-implant-dentistry
#12
Nils-Claudius Gellrich, Rüdiger M Zimmerer, Simon Spalthoff, Philipp Jehn, Philipp-Cornelius Pott, Majeed Rana, Björn Rahlf
OBJECTIVES: Numerous procedures including soft and hard tissue reconstructions are necessary to allow for final implant-borne dental rehabilitation in patients with severe alveolar atrophy or resected jaws. In these cases, customised digitally engineered patient solutions for fixed or removable dental rehabilitation provide an innovative line extension to conventional implant dentistry. MATERIAL AND METHODS: A wax-up was performed on plaster models that were scanned by 3D laser...
August 3, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28862611/phase-merging-enhanced-harmonic-generation-free-electron-laser-with-a-normal-modulator
#13
Zhouyu Zhao, Heting Li, Qika Jia
A phase-merging enhanced harmonic generation free-electron laser (FEL) was proposed to increase the harmonic conversion efficiency of seeded FELs and promote the radiation wavelength towards the X-ray spectral region. However, this requires a specially designed transverse gradient undulator (TGU) as the modulator to couple the transverse and longitudinal phase space of the electron beam. In this paper, the generation of the phase-merging effect is explored using the natural field gradient of a normal planar undulator...
September 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28859710/the-effect-of-closed-septorhinoplasty-on-nasal-functions-and-on-external-and-internal-nasal-valves-a-prospective-study
#14
Giancarlo Pecorari, Giuseppe Riva, Francesca Antonella Bianchi, Giovanni Cavallo, Francesca Revello, Marco Bironzo, Laura Verzè, Massimiliano Garzaro, Guglielmo Ramieri
BACKGROUND: Because nasal function and shape are so closely intertwined, quantitative assessments can better define their relationship and how they are affected by septorhinoplasty. OBJECTIVE: The aim of this prospective study was to perform an analysis of the nasal airflow resistances and a three-dimensional (3D) evaluation of the soft-tissue changes after closed septorhinoplasty. METHODS: Before surgery (T0) and 6 months after closed septorhinoplasty (T1), 30 patients underwent symptoms evaluation by means of the Italian version of the Nasal Obstruction Symptom Evaluation scale, endoscopic fiberoptic nasal examination, and visual analog scale for subjective assessment of nasal obstruction...
September 1, 2017: American Journal of Rhinology & Allergy
https://www.readbyqxmd.com/read/28856052/differentiation-of-fibrotic-liver-tissue-using-laser-induced-breakdown-spectroscopy
#15
E Teran-Hinojosa, H Sobral, C Sánchez-Pérez, A Pérez-García, N Alemán-García, J Hernández-Ruiz
Hepatic cirrhosis is a major cause of morbidity and mortality worldwide due to hepatitis C, alcoholism and fatty liver disease associated with obesity. Assessment of hepatic fibrosis relies in qualitative histological evaluation of biopsy samples. This method is time-consuming and depends on the histopathologists' interpretation. In the last decades, non-invasive techniques were developed to detect and monitor hepatic fibrosis. Laser-induced breakdown spectroscopy (LIBS) is a good candidate for a real-time, independent and fast technique to diagnose hepatic fibrosis...
August 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28852689/time-resolved-soft-x-ray-absorption-spectroscopy-in-transmission-mode-on-liquids-at-mhz-repetition-rates
#16
Mattis Fondell, Sebastian Eckert, Raphael M Jay, Christian Weniger, Wilson Quevedo, Johannes Niskanen, Brian Kennedy, Florian Sorgenfrei, Daniel Schick, Erika Giangrisostomi, Ruslan Ovsyannikov, Katrin Adamczyk, Nils Huse, Philippe Wernet, Rolf Mitzner, Alexander Föhlisch
We present a setup combining a liquid flatjet sample delivery and a MHz laser system for time-resolved soft X-ray absorption measurements of liquid samples at the high brilliance undulator beamline UE52-SGM at Bessy II yielding unprecedented statistics in this spectral range. We demonstrate that the efficient detection of transient absorption changes in transmission mode enables the identification of photoexcited species in dilute samples. With iron(II)-trisbipyridine in aqueous solution as a benchmark system, we present absorption measurements at various edges in the soft X-ray regime...
September 2017: Structural Dynamics (Melville, N.Y.)
https://www.readbyqxmd.com/read/28852688/euv-stimulated-emission-from-mgo-pumped-by-fel-pulses
#17
Philippe Jonnard, Jean-Michel André, Karine Le Guen, Meiyi Wu, Emiliano Principi, Alberto Simoncig, Alessandro Gessini, Riccardo Mincigrucci, Claudio Masciovecchio, Olivier Peyrusse
Stimulated emission is a fundamental process in nature that deserves to be investigated and understood in the extreme ultra-violet (EUV) and x-ray regimes. Today, this is definitely possible through high energy density free electron laser (FEL) beams. In this context, we give evidence for soft-x-ray stimulated emission from a magnesium oxide solid target pumped by EUV FEL pulses formed in the regime of travelling-wave amplified spontaneous emission in backward geometry. Our results combine two effects separately reported in previous works: emission in a privileged direction and existence of a material-dependent threshold for the stimulated emission...
September 2017: Structural Dynamics (Melville, N.Y.)
https://www.readbyqxmd.com/read/28842005/new-procedure-of-quantitative-mapping-of-ti-and-al-released-from-dental-implant-and-mg-ca-fe-zn-cu-mn-as-physiological-elements-in-oral-mucosa-by-la-icp-ms
#18
Adam Sajnóg, Anetta Hanć, Ryszard Koczorowski, Danuta Barałkiewicz
A new procedure for determination of elements derived from titanium implants and physiological elements in soft tissues by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented. The analytical procedure was developed which involved preparation of in-house matrix matched solid standards with analyte addition based on certified reference material (CRM) MODAS-4 Cormorant Tissue. Addition of gelatin, serving as a binding agent, essentially improved physical properties of standards...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28829571/bioinspired-programmable-polymer-gel-controlled-by-swellable-guest-medium
#19
Heng Deng, Yuan Dong, Jheng-Wun Su, Cheng Zhang, Yunchao Xie, Chi Zhang, Matthew R Maschmann, Yuyi Lin, Jian Lin
Responsive materials with functions of forming three-dimensional (3D) origami and/or kirigami structures have a broad range of applications in bioelectronics, metamaterials, microrobotics, and microelectromechanical (MEMS) systems. To realize such functions, building blocks of actuating components usually possess localized inhomogeneity so that they respond differently to external stimuli. Previous fabrication strategies lie in localizing nonswellable or less-swellable guest components in their swellable host polymers to reduce swelling ability...
September 13, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28828162/the-application-of-diode-laser-in-the-treatment-of-oral-soft-tissues-lesions-a-literature-review
#20
REVIEW
Daniel Ortega-Concepción, Jorge A Cano-Durán, Juan-Francisco Peña-Cardelles, Víctor-Manuel Paredes-Rodríguez, José González-Serrano, Juan López-Quiles
BACKGROUND: Since its appearance in the dental area, the laser has become a treatment of choice in the removal of lesions in the oral soft tissues, due to the numerous advantages they offer, being one of the most used currently the diode laser. The aim of this review was to determine the efficacy and predictability of diode laser as a treatment of soft tissue injuries compared to other surgical methods. MATERIAL AND METHODS: A literature review of articles published in PubMed/MEDLINE, Scopus and the Cochrane Library databases between 2007 and 2017 was performed...
July 2017: Journal of Clinical and Experimental Dentistry
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