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https://www.readbyqxmd.com/read/28545064/transcriptomic-analysis-of-the-response-of-pseudomonas-fluorescens-to-epigallocatechin-gallate-by-rna-seq
#1
Xiaoxiang Liu, Bimiao Shen, Peng Du, Nan Wang, Jiaxue Wang, Jianrong Li, Aihua Sun
Epigallocatechin gallate (EGCG) is a main constituent of green tea polyphenols that are widely used as food preservatives and are considered to be safe for consumption. However, the underlying antimicrobial mechanism of EGCG and the bacterial response to EGCG are not clearly understood. In the present study, a genome-wide transcriptional analysis of a typical spoilage bacterium, Pseudomonas fluorescens that responded to EGCG was performed using RNA-seq technology. A total of 26,365,414 and 23,287,092 clean reads were generated from P...
2017: PloS One
https://www.readbyqxmd.com/read/28544528/micellar-electrokinetic-capillary-chromatography-fingerprints-combined-with-multivariate-statistical-analyses-to-evaluate-the-quality-consistency-and-predict-the-fingerprint-efficacy-relationship-of-salviae-miltiorrhizae-radix-et-rhizoma-danshen
#2
Yujing Zhang, Lanping Yang, Jing Zhang, Min Shi, Guoxiang Sun
Micellar electrokinetic capillary chromatography fingerprinting combined with quantification was successfully established and applied to evaluate the quality consistency of Danshen, which is a medicinal herb used to treat various diseases, especially coronary cerebrovascular diseases. A background electrolyte composed of 20 mmol/L sodium tetraborate, 90 mmol/L orthoboric acid, 25 mmol/L sodium phosphate monobasic dehydrate and 65 mmol/L sodium dodecyl sulfate was used to separate compounds. To optimize micellar electrokinetic capillary chromatography conditions, a response surface strategy was set up for orthogonal experimental design...
May 22, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28544098/crystal-structure-of-the-sensor-domain-of-baes-from-serratia-marcescens-fs14
#3
Ya Zhang, Shenshen Qiu, Shanshan Jia, Dongqing Xu, Tingting Ran, Weiwu Wang
The sensor histidine kinases of two-component signal-transduction systems (TCSs) are essential for bacteria to adapt to variable environmental conditions. The two-component regulatory system BaeS/R increases multidrug and metal resistance in Salmonella and Escherichia coli. In this study, we report the X-ray structure of the periplasmic sensor domain of BaeS from Serratia marcescens FS14. The BaeS sensor domain (34-160) adopts a mixed α/β-fold containing a central four-stranded antiparallel β-sheet flanked by a long N-terminal α-helix and additional loops and a short C-terminal α-helix on each side...
May 24, 2017: Proteins
https://www.readbyqxmd.com/read/28543514/angioimmunoblastic-t-cell-lymphoma-more-than-a-disease-of-t-follicular-helper-cells
#4
François Lemonnier, Tak W Mak
Angioimmunoblastic T cell lymphoma (AITL) is one of the most frequent entities of peripheral T cell lymphoma. An AITL has two components: the AITL tumor cells that have a T follicular helper (TFH) cell phenotype, and a surrounding and extensive tumor microenvironment that is populated with various reactive cell types, including B cells. Recurrent TET2 mutations have been described in 50-80% of AITLs, possibly occurring in a hematopoietic progenitor cell. An article published recently in The Journal of Pathology describes the use of microdissection to isolate PD1(+) AITL tumor cells and CD20(+) B cells from the AITL microenvironment and to show that TET2 mutations are actually more frequent in these diseases than previously thought...
May 24, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28542616/regulation-of-acetyl-coa-synthetase-transcription-by-the-crbs-r-two-component-system-is-conserved-in-genetically-diverse-environmental-pathogens
#5
Kristin Jacob, Anna Rasmussen, Paul Tyler, Mariah M Servos, Mariame Sylla, Cecilia Prado, Elizabeth Daniele, Josh S Sharp, Alexandra E Purdy
The CrbS/R two-component signal transduction system is a conserved regulatory mechanism through which specific Gram-negative bacteria control acetate flux into primary metabolic pathways. CrbS/R governs expression of acetyl-CoA synthase (acsA), an enzyme that converts acetate to acetyl-CoA, a metabolite at the nexus of the cell's most important energy-harvesting and biosynthetic reactions. During infection, bacteria can utilize this system to hijack host acetate metabolism and alter the course of colonization and pathogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28542449/specific-expression-and-function-of-the-a-type-cytochrome-c-oxidase-under-starvation-conditions-in-pseudomonas-aeruginosa
#6
Tatsuya Osamura, Takuro Kawakami, Reiko Kido, Masaharu Ishii, Hiroyuki Arai
Pseudomonas aeruginosa has one A-type (caa3) and multiple C-type (cbb3) cytochrome c oxidases as well as two quinol oxidases for aerobic respiration. The caa3 oxidase is highly efficient in creating a proton gradient across the cell membrane, but it is not expressed under normal growth conditions and its physiological role has not been investigated. In the present study, a mutant strain deficient in the coxBA-PA0107-coxC genes encoding caa3 exhibited normal growth under any test conditions, but it had low relative fitness under carbon starvation conditions, indicating that the expression of caa3 is advantageous under starvation conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28541480/alternative-evolutionary-paths-to-bacterial-antibiotic-resistance-cause-distinct-collateral-effects
#7
C Barbosa, V Trebosc, C Kemmer, P Rosenstiel, R Beardmore, H Schulenburg, G Jansen
When bacteria evolve resistance against a particular antibiotic, they may simultaneously gain increased sensitivity against a second one. Such collateral sensitivity may be exploited to develop novel, sustainable antibiotic treatment strategies aimed at containing the current, dramatic spread of drug resistance. To date, the presence and molecular basis of collateral sensitivity has only been studied in few bacterial species and is unknown for opportunistic human pathogens such as Pseudomonas aeruginosa. In the present study, we assessed patterns of collateral effects by experimentally evolving 160 independent populations of P...
May 23, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28540720/specifically-increased-paclitaxel-release-in-tumor-and-synergetic-therapy-by-a-hyaluronic-acid-tocopherol-nanomicelle
#8
Hanbo Zhang, Wei Li, Xiaomeng Guo, Fenfen Kong, Zuhua Wang, Chunqi Zhu, Lihua Luo, Qingpo Li, Jie Yang, Yongzhong Du, Jian You
Recently, interest in tumor-targeting and site-specific drug release from nanoparticles as a means of drug delivery has increased. In this study, we reported on a smart nanosized micelle formed by hyaluronic acid (HA) conjugated with D-α-Tocopherol Succinate (TOS) using a disulfide bond as the linker (HA-SS-TOS, HSST). HSST micelles can specifically bind to the CD44 receptors that are overexpressed on cancer cells. The high levels of glutathione (GSH) in tumor cells selectively break the disulfide bond linker...
May 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28539658/functional-compressive-mechanics-and-tissue-biocompatibility-of-an-injectable-sf-pu-hydrogel-for-nucleus-pulposus-replacement
#9
Jingen Hu, Yang Lu, Ling Cai, Kwabena Gyabaah Owusu-Ansah, Gewen Xu, Feilong Han, Junjie Bao, Xiangjin Lin, Yiping Huang
In spinal degenerative disease, an injectable liquid hydrogel can fill in defect entirely, lessen the danger of implant relocation and following loss of disc height, minimizing the operative trauma. Here, we propose an injectable in-situ chemically cross-linked hydrogel by a two-component reaction of liquid silk fibroin with liquid polyurethane at physiological temperature conditions. Confined compression tests and fatigue tests were reported to assess physical properties of the hydrogel. Impact of different diameter on the biomechanical behaviours was tested to evaluate the clinical potentiality of the hydrogel for replacing nucleus pulposus...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28539339/mif-2-d-dt-enhances-proximal-tubular-cell-regeneration-through-slpi-and-atf4-dependent-mechanisms
#10
Akinobu Ochi, Dong Chen, Wibke Schulte, Lin Leng, Nickolas Gilbert Moeckel, Marta Piecychna, Luisa Averdunk, Christian Stoppe, Richard Bucala, Gilbert W Moeckel
Macrophage migration inhibitory factor (MIF) is a cytokine with pleiotropic actions that is produced by several organs and cell types. Depending on the target cell and the inflammatory context, MIF can engage its two component receptor complex CD74 and CD44, and the chemokine receptors CXCR2/4. MIF is constitutively expressed in renal proximal tubular cells, stored in intracellular preformed pools and is released at a low rate. Recently a second MIF-like protein (i.e. MIF-2/D-DT) has been characterized in mammals...
May 24, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28536712/scaleable-two-component-gelator-from-phthalic-acid-derivatives-and-primary-alkyl-amines-acid-base-interaction-in-the-cooperative-assembly
#11
Ting Su, Kwon Ho Hong, Wannian Zhang, Fei Li, Qiang Li, Fang Yu, Genxiang Luo, Honghe Gao, Yu-Peng He
A series of phthalic acid derivatives (P) with a carbon-chain tail was designed and synthesized as single-component gelators. A combination of the single-component gelator P and a non-gelling additive n-alkylamine A through acid-base interaction brought about a series of novel phase-selective two-component gelators PA. The gelation capabilities of P and PA, and the structural, morphological, thermo-dynamic and rheological properties of the corresponding gels were investigated. A molecular dynamics simulation showed that the H-bonding network in PA formed between the NH of A and the carbonyl oxygen of P altered the assembly process of gelator P...
May 24, 2017: Soft Matter
https://www.readbyqxmd.com/read/28536675/an-overview-of-two-component-signal-transduction-systems-implicated-in-extra-intestinal-pathogenic-e-coli-infections
#12
REVIEW
Erin J Breland, Allison R Eberly, Maria Hadjifrangiskou
Extra-intestinal pathogenic E. coli (ExPEC) infections are common in mammals and birds. The predominant ExPEC types are avian pathogenic E. coli (APEC), neonatal meningitis causing E. coli/meningitis associated E. coli (NMEC/MAEC), and uropathogenic E. coli (UPEC). Many reviews have described current knowledge on ExPEC infection strategies and virulence factors, especially for UPEC. However, surprisingly little has been reported on the regulatory modules that have been identified as critical in ExPEC pathogenesis...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28535670/ultrafast-spectroscopy-reveals-electron-transfer-cascade-that-improves-hydrogen-evolution-with-carbon-nitride-photocatalysts
#13
Kathryn L Corp, Cody W Schlenker
Solar hydrogen generation from water represents a compelling component of a future sustainable energy portfolio. Recently, chemically-robust heptazine-based polymers known as graphitic carbon nitrides (g-C3N4) have emerged as promising photocatalysts for hydrogen evolution using visible light while withstanding harsh chemical environments. However, since g-C3N4 electron transfer dynamics are poorly understood, rational design rules for improving activity remain unclear. Here, we use visible and near-infrared femtosecond transient absorption (TA) spectroscopy to reveal an electron transfer cascade that correlates with a near-doubling in photocatalytic activity from 2050 µmol h(-1)g(-1) to 3810 µmol h(-1)g(-1) when we infuse a suspension of bulk g-C3N4 with 10% mass loading of chemically exfoliated carbon nitride...
May 23, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28535488/ftir-analysis-on-aging-characteristics-of-abs-pc-blend-under-uv-irradiation-in-air
#14
Jiarong Li, Fu Chen, Long Yang, Long Jiang, Yi Dan
Fourier Transform Infrared Spectroscopy (FTIR) is adopted to study the aging characteristics of poly(acrylonitrile-butadiene-styrene)/polycarbonate (ABS/PC) blend under UV-irradiation in air by analyzing the variation of the three main absorbance at about 967cm(-1), 1720cm(-1) and 3420cm(-1) associated with carbon-hydrogen bonds belonging to 1,4 butadiene, carbonyl and hydroxyl groups, respectively. Results indicate that, under UV-irradiation in air, the photo-oxidation of the blend is not a simple combination of the photo-oxidation of corresponding ABS and PC themselves and takes place predominantly at the ABS component...
April 29, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28535261/recruitment-of-a-lineage-specific-virulence-regulatory-pathway-promotes-intracellular-infection-by-a-plant-pathogen-experimentally-evolved-into-a-legume-symbiont
#15
Delphine Capela, Marta Marchetti, Camille Clérissi, Anthony Perrier, Dorian Guetta, Carine Gris, Marc Valls, Alain Jauneau, Stéphane Cruveiller, Eduardo P C Rocha, Catherine Masson-Boivin
Ecological transitions between different lifestyles, such as pathogenicity, mutualism and saprophytism, have been very frequent in the course of microbial evolution, and often driven by horizontal gene transfer. Yet, how genomes achieve the ecological transition initiated by the transfer of complex biological traits remains poorly known. Here we used experimental evolution, genomics, transcriptomics and high-resolution phenotyping to analyze the evolution of the plant pathogen Ralstonia solanacearum into legume symbionts, following the transfer of a natural plasmid encoding the essential mutualistic genes...
May 23, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28533567/synthesis-and-incorporation-of-a-caged-tyrosine-amino-acid-possessing-a-bioorthogonal-handle
#16
Marshall S Padilla, Christopher A Farley, Lindsay E Chatkewitz, Douglas D Young
Reversing a bioconjugation in a spatial and temporal fashion has widespread applications, especially toward targeted drug delivery. We report the synthesis and incorporation of an unnatural amino acid with an alkyne modified dimethoxy-ortho-nitrobenzyl caging group. This unnatural amino acid can be utilized in a Glaser-Hay conjugation to generate a bioconjugate, but also is able to disrupt the bioconjugate when irradiated with light. These combined features allow for the preparation of bioconjugates with a high degree of site-specificity and allow for the separation of the two components if necessary...
October 19, 2016: Tetrahedron Letters
https://www.readbyqxmd.com/read/28533471/the-atypical-response-regulator-atvr-is-a-new-player-in-pseudomonas-aeruginosa-response-to-hypoxia-and-virulence
#17
Gilberto Hideo Kaihami, Leandro Carvalho Dantas Breda, José Roberto Fogaça de Almeida, Thays de Oliveira Pereira, Gianlucca Gonçalves Nicastro, Ana Laura Boechat, Sandro Rogério de Almeida, Regina Lúcia Baldini
Two-component systems are widespread in bacteria, allowing adaptation to environmental changes. The classical pathway is composed by a histidine kinase that phosphorylates an aspartate residue in the cognate response regulator (RR). RRs lacking the phosphorylable aspartate also occur, but their function and contribution during host-pathogen interaction are poorly characterized. AtvR (PA14_26570) is the only atypical response regulator with a DNA-binding domain in the opportunistic pathogen Pseudomonas aeruginosa Macrophage infection with the atvR mutant strain resulted in higher TNF-α secretion as well as increased bacterial clearance as compared to macrophages infected with the wild-type strain...
May 22, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28533218/involvement-of-two-component-signaling-in-bacterial-motility-and-biofilm-development
#18
Birgit M Prüβ
Two-component signaling is a specialized mechanism that bacteria use to respond to changes in their environment. Non-pathogenic strains of E. coli K-12 harbor 30 histidine kinases and 32 response regulators, which form a network of regulation that integrates many other global regulators that do not follow the two-component signaling mechanism, as well as signals from central metabolism. The output of this network is a multitude of phenotypic changes in response to changes in the environment. Among these phenotypic changes, many two-component systems control motility and/or the formation of biofilm, sessile communities of bacteria that form on surfaces...
May 22, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28533217/transcriptional-control-of-the-lateral-flagellar-genes-of-bradyrhizobium-diazoefficiens
#19
Elías J Mongiardini, J Ignacio Quelas, Carolina Dardis, M Julia Althabegoiti, Aníbal R Lodeiro
Bradyrhizobium diazoefficiens, the soybean N2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. Here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. We found that those genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. Among the monocistronic units is blr6846, homologous to the Class-IB master regulators of flagella synthesis in Brucella melitensis and Ensifer meliloti, and required for the expression of all the lateral-flagellar genes except lafA2, which locus encodes a single lateral flagellin...
May 22, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28531879/scaffold-requirements-for-periodontal-regeneration-with-enamel-matrix-derivative-proteins
#20
Alessandra Apicella, Peggy Heunemann, Laurent Dejace, Matteo Marascio, Christopher J G Plummer, Peter Fischer
Periodontitis affects the attachment of natural teeth, and infection or inflammation associated with periodontitis may affect peri-implant tissues. Enamel matrix derivative (EMD) proteins provide stimulation for self-regeneration of the damaged tissue when applied to wide intrabony defects as part of a mixture with bone graft material. As a first step of the process enhancing cell proliferation and ligament formation, we demonstrated that EMD protein precipitation depends strongly on the physical and chemical characteristics of the bone grafts used in the mixture...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
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