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Histidine kinase

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https://www.readbyqxmd.com/read/28331163/in-vitro-evaluation-of-candidate-bacillus-spp-for-animal-feed
#1
Sirima Mingmongkolchai, Watanalai Panbangred
The aim of this study was to select aerobic spore-formers for animal feed based on their in vitro probiotic potential, including their enzyme-producing ability and safety assessment. Seven isolates out of 187 spore-forming bacteria were selected for their ability to produce cellulase (89.21-1668.32 U/ml), xylanase (1399.68-4351.10 U/ml), and phytase (2.72-28.70 U/ml). Among seven isolates, five had activities towards a broad range of p-nitrophenyl esters with acyl chain lengths from C2 to C12. The probiotic properties of all selected isolates varied with respect to their acid and bile salt tolerance under simulated gastrointestinal tract (GIT) conditions, and their adherence ability to human intestinal cell lines (Caco-2 and HT-29)...
March 22, 2017: Journal of General and Applied Microbiology
https://www.readbyqxmd.com/read/28295748/role-of-the-sln1-phosphorelay-pathway-in-the-response-to-hyperosmotic-stress-in-the-yeast-kluyveromyces-lactis
#2
Miriam Rodríguez-González, Laura Kawasaki, Nancy Velázquez-Zavala, Eunice Domínguez-Martín, Abraham Trejo-Medecigo, Natalia Martagón, Emilio Espinoza-Simón, Araceli Vázquez-Ibarra, Laura Ongay-Larios, Dimitris Georgellis, Eulàlia de Nadal, Francesc Posas, Roberto Coria
The Kluyveromyces lactis SLN1 phosphorelay system includes the osmosensor histidine kinase Sln1, the phosphotransfer protein Ypd1 and the response regulator Ssk1. Here we show that K. lactis has a functional phosphorelay system. In vitro assays, using a heterolougus histidine kinase, show that the phosphate group is accepted by KlYpd1 and transfer to KlSsk1. Upon hyperosmotic stress the phosphorelay is inactivated, KlYpd1 is dephosphorylated in a KlSln1 dependent manner, and only the version of KlSsk1 that lacks the phosphate group interacts with the MAPKKK KlSsk2...
March 13, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28293894/primer-aided-truncation-for-the-creation-of-hybrid-proteins
#3
Robert Stabel, Birthe Stüven, Robert Ohlendorf, Andreas Möglich
Proteins frequently display modular architecture with several domains and segments connected by linkers. Proper protein functionality hinges on finely orchestrated interactions among these constituent elements. The underlying modularity lends itself to the engineering of hybrid proteins via modular rewiring; novel properties can thus be obtained, provided the linkers connecting the individual elements are conducive to productive interactions. As a corollary, the process of protein engineering often encompasses the generation and screening of multiple linker variants...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293842/analysis-of-ethylene-receptors-assay-for-histidine-kinase-activity
#4
G Eric Schaller, Brad M Binder
The ethylene receptors of plants exist in two subfamilies. Members of subfamily-1 have functional histidine kinase domains, whereas members of subfamily-2 have diverged histidine-kinase-like domains that in some cases have been shown to exhibit Ser/Thr kinase activity. Here, we describe a method to biochemically characterize the enzymatic activity of these kinase domains in vitro. For this purpose, the histidine kinase domain of the receptors is transgenically expressed in yeast as a fusion to glutathione-S-transferase (GST) for subsequent affinity purification...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28292856/light-regulated-expression-of-sensor-histidine-kinase-cki1-controls-cytokinin-related-development
#5
Tereza Dobisova, Vendula Hrdinova, Candela Cuesta, Sarka Michlickova, Ivana Urbankova, Romana Hejatkova, Petra Žádníková, Marketa Pernisova, Eva Benkova, Jan Hejatko
In plants, the multistep phosphorelay (MSP) pathway mediates a range of regulatory processes, including those activated by cytokinins. The crosstalk between cytokinin response and light is known for a long time. However, the molecular mechanism underlying the interactionbetween light and cytokinin signaling remains elusive. In the screen for upstream regulators we identified a LONG PALE HYPOCOTYL (LPH) gene whose activity is indispensable for spatiotemporally correct expression of CYTOKININ INDEPENDENT-1 (CKI1), encoding the constitutively active sensor histidine kinase that activates MSP signaling...
March 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28289094/inter-subunit-distances-in-full-length-dimeric-bacterial-phytochrome-agp1-as-measured-by-peldor-between-different-spin-label-positions-remain-unchanged-upon-photoconversion
#6
Sylwia Kacprzak, Ibrahim Nijmona, Anja Renz, Juan Feng, Edward Reijerse, Wolfgang Lubitz, Norbert Krau Szlig, Patrick Scheerer, Soshichiro Nagano, Tilman Lamparter, Stefan Weber
Bacterial phytochromes are dimeric light-regulated histidine kinases that convert red light into signaling events. Light absorption by the N-terminal photosensory core module (PCM) causes the proteins to switch between two spectrally distinct forms, Pr and Pfr, thus resulting in a conformational change that modulates the C-terminal histidine kinase region. To provide further insights into structural details of photoactivation, we investigated the full-length Agp1 bacteriophytochrome from the soil bacterium Agrobacterium fabrum using a combined spectroscopic and modeling approach...
March 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28265100/sensory-domain-contraction-in-histidine-kinase-cita-triggers-transmembrane-signaling-in-the-membrane-bound-sensor
#7
Michele Salvi, Benjamin Schomburg, Karin Giller, Sabrina Graf, Gottfried Unden, Stefan Becker, Adam Lange, Christian Griesinger
Bacteria use membrane-integral sensor histidine kinases (HK) to perceive stimuli and transduce signals from the environment to the cytosol. Information on how the signal is transmitted across the membrane by HKs is still scarce. Combining both liquid- and solid-state NMR, we demonstrate that structural rearrangements in the extracytoplasmic, citrate-sensing Per-Arnt-Sim (PAS) domain of HK CitA are identical for the isolated domain in solution and in a longer construct containing the membrane-embedded HK and lacking only the kinase core...
March 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28264855/structural-alteration-of-ompr-as-a-source-of-ertapenem-resistance-in-a-ctx-m-15-producing-escherichia-coli-o25b-h4-st131-clinical-isolate
#8
Hervé Dupont, Pascaline Choinier, David Roche, Sandine Adiba, Megan Sookdeb, Catherine Branger, Erick Denamur, Hedi Mammeri
In this study, an ertapenem-non-susceptible Escherichia coli isolate was investigated to determine the genetic basis for its carbapenem resistance phenotype. This clinical strain was recovered from a patient that received, one year ago, ertapenem to treat a cholangitis due to a carbapenem-susceptible extended-spectrum β-lactamase (ESBL)-producing E. coli isolate. Whole-genome sequencing of these strains was performed using Illumina and single molecule real-time sequencing technologies. It revealed that they belonged to the ST131 clonal group, had the predicted O25b:H4 serotype, and produced the CTX-M-15 and TEM-1 β-lactamases...
March 6, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28249794/identification-of-mycobacterial-gara-as-a-substrate-of-protein-kinase-g-from-m-tuberculosis-using-a-kestrel-based-proteome-wide-approach
#9
Philipp Mueller, Jean Pieters
Signal transduction in bacteria is generally mediated via two-component systems. These systems depend on the transfer of a phosphate molecule from a donor to an acceptor by histidine kinases, thereby activating the acceptor to allow downstream signaling/activation. Several bacterial genomes, including the genome of M. tuberculosis, were shown to encode eukaryotic-like kinases. To better understand the function of these kinases and the regulatory networks within which they operate, identification of downstream targets is essential...
February 27, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28246363/a-localized-complex-of-two-protein-oligomers-controls-the-orientation-of-cell-polarity
#10
Adam M Perez, Thomas H Mann, Keren Lasker, Daniel G Ahrens, Michael R Eckart, Lucy Shapiro
Signaling hubs at bacterial cell poles establish cell polarity in the absence of membrane-bound compartments. In the asymmetrically dividing bacterium Caulobacter crescentus, cell polarity stems from the cell cycle-regulated localization and turnover of signaling protein complexes in these hubs, and yet the mechanisms that establish the identity of the two cell poles have not been established. Here, we recapitulate the tripartite assembly of a cell fate signaling complex that forms during the G1-S transition...
February 28, 2017: MBio
https://www.readbyqxmd.com/read/28244240/mst50-is-involved-in-multiple-map-kinase-signaling-pathways-in-magnaporthe-oryzae
#11
Guotian Li, Xue Zhang, Huan Tian, Yoon-E Choi, W Andy Tao, Jin-Rong Xu
Appressorium formation plays a critical role in Magnaporthe oryzae. Mst50 is an adapter protein of the Mst11-Mst7-Pmk1 cascade that is essential for appressorium formation. To further characterize its functions, affinity purification was used to identify Mst50-interacting proteins (MIPs) in this study. Two of the MIPs are Mst11 and Mst7 that are known to interact with Mst50 for Pmk1 activation. Surprisingly, two other MIPs are Mck1 and Mkk2 that are the upstream kinases of the Mps1 pathway. Domain deletion analysis showed that the sterile alpha-motif of Mst50 but not the Ras-association domain was important for its interaction with Mck1 and responses to cell wall and oxidative stresses...
February 28, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28242664/the-two-component-system-chtrs-contributes-to-chlorhexidine-tolerance-in-enterococcus-faecium
#12
Ana M Guzmán Prieto, Jessica Wijngaarden, Johanna C Braat, Malbert R C Rogers, Eline Majoor, Ellen C Brouwer, Xinglin Zhang, Jumamurat R Bayjanov, Marc J M Bonten, Rob J L Willems, Willem van Schaik
Enterococcus faecium is one of the primary causes of nosocomial infections. Dinsinfectants are commonly used to prevent infections with multidrug-resistant E. faecium in hospitals. Worryingly, E. faecium strains that exhibit tolerance to disinfectants have already been described. We aimed to identify and characterize E. faecium genes that contribute to tolerance to the disinfectant chlorhexidine (CHX).We used a transposon mutant library, constructed in a multi-drug resistant E. faecium bloodstream isolate, to perform a genome-wide screen to identify genetic determinants involved in tolerance to CHX...
February 27, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28238256/discovery-of-a-novel-nitric-oxide-binding-protein-and-nitric-oxide-responsive-signaling-pathway-in-pseudomonas-aeruginosa
#13
Sajjad Hossain, Elizabeth M Boon
Nitric oxide (NO) is a radical diatomic gas molecule that, at low concentrations, plays important signaling roles in both eukaryotes and bacteria. In recent years, it has become evident that bacteria respond to low levels of NO in order to modulate their group behavior. Many bacteria respond via NO ligation to a well-established NO sensor called H-NOX (heme-nitric oxide/oxygen binding domain). Many others, such as Pseudomonas aeruginosa, lack an annotated hnoX gene in their genome yet are able to respond to low levels of NO to disperse their biofilms...
March 16, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28237997/umtco1-a-hybrid-histidine-kinase-gene-is-essential-for-the-sexual-development-and-virulence-of-ustilago-maydis
#14
Yeo Hong Yun, Man Hwan Oh, Jun Young Kim, Seong Hwan Kim
Hybrid histidine kinase is a part of two-component system that is required for various stress responses and pathogenesis of pathogenic fungi. The Tco1 gene in human pathogen Cryptococcus neoformans encodes a hybrid histidine kinase and is important for pathogenesis. In the present study, we identified a Tco1 gene homolog, UmTco1, in the corn smut pathogen Ustilago maydis by bioinformatics analysis. To explore the role of UmTco1 in the survival of U. maydis under environmental stresses and its pathogenesis, Δumtco1 mutants were constructed by allelic exchange...
February 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28217823/novel-insight-from-computational-virtual-screening-depict-the-binding-potential-of-selected-phytotherapeutics-against-probable-drug-targets-of-clostridium-difficile
#15
Suman Kamath, Sinosh Skariyachan
This study explores computational screening and molecular docking approaches to screen novel herbal therapeutics against probable drug targets of Clostridium difficile. The essential genes were predicted by comparative genome analysis of C. difficile and best homologous organisms using BLAST search at database of essential genes (DEG). The functions of these genes in various metabolic pathways were predicted and some of these genes were considered as potential targets. Three major proteins were selected as putative targets, namely permease IIC component, ABC transporter and histidine kinase...
February 20, 2017: Interdisciplinary Sciences, Computational Life Sciences
https://www.readbyqxmd.com/read/28197164/isolation-characterization-and-transcriptome-analysis-of-a-cytokinin-receptor-mutant-osckt1-in-rice
#16
Wona Ding, Huishan Tong, Wenjuan Zheng, Jing Ye, Zhichong Pan, Botao Zhang, Shihua Zhu
Cytokinins play important roles in regulating plant development, including shoot and root meristems, leaf longevity, and grain yield. However, the in planta functions of rice cytokinin receptors have not been genetically characterized yet. Here we isolated a rice mutant, Osckt1, with enhanced tolerance to cytokinin treatment. Further analysis showed that Osckt1 was insensitive to aromatic cytokinins but responded normally to isoprenoid and phenylurea-type cytokinins. Map-based cloning revealed that the mutation occurred in a putative cytokinin receptor gene, histidine kinase 6 (OsHK6)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28196956/amino-acid-catabolism-in-staphylococcus-aureus-and-the-function-of-carbon-catabolite-repression
#17
Cortney R Halsey, Shulei Lei, Jacqueline K Wax, Mckenzie K Lehman, Austin S Nuxoll, Laurey Steinke, Marat Sadykov, Robert Powers, Paul D Fey
Staphylococcus aureus must rapidly adapt to a variety of carbon and nitrogen sources during invasion of a host. Within a staphylococcal abscess, preferred carbon sources such as glucose are limiting, suggesting that S. aureus survives through the catabolism of secondary carbon sources. S. aureus encodes pathways to catabolize multiple amino acids, including those that generate pyruvate, 2-oxoglutarate, and oxaloacetate. To assess amino acid catabolism, S. aureus JE2 and mutants were grown in complete defined medium containing 18 amino acids but lacking glucose (CDM)...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28193573/structure-and-function-of-hwe-hiska2-family-sensor-histidine-kinases
#18
REVIEW
Julien Herrou, Sean Crosson, Aretha Fiebig
Sensor histidine kinases regulate adaptive cellular responses to changes in the chemical or physical state of the environment. HWE/HisKA2-family kinases comprise a subset of histidine kinases that is defined by unique sequence motifs in both the catalytic and non-catalytic regions. Recent crystal structures have defined conserved intramolecular interactions that inform models of kinase regulation that are unique to the HWE/HisKA2 superfamily. Emerging genetic, biochemical and genomic data indicate that, unlike typical histidine kinases, HWE/HisKA2 kinases do not generally signal via classical DNA-binding response regulators...
February 10, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28184221/identification-of-key-residues-in-the-nisk-sensor-region-for-nisin-biosynthesis-regulation
#19
Xiaoxuan Ge, Kunling Teng, Jian Wang, Fangyuan Zhao, Jie Zhang, Jin Zhong
Histidine kinase (HK) NisK is well known to sense lantibiotic nisin for regulating the biosynthesis of nisin. NisK possesses two trans-membrane segments and a large extracellular region and nisin contains 34 amino acids with five lanthionine rings. Unlike most peptide sensing HK with multi trans-membrane segments, NisK is a representative of a group of rarely reported HK that sense peptide as ligand. To reveal how NisK senses nisin molecule to regulate nisin biosynthesis, we constructed a reporter Lactococcus lactis strain with nisRK constitutively expressed and a reporter gene lacZ expressed under the control of promoter P nisA ...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28170222/nitric-oxide-induced-conformational-changes-govern-h-nox-and-histidine-kinase-interaction-and-regulation-in-shewanella-oneidensis
#20
Minxi Rao, Mark A Herzik, Anthony T Iavarone, Michael A Marletta
Nitric oxide (NO) is implicated in biofilm regulation in several bacterial families via heme-nitric oxide/oxygen binding (H-NOX) protein signaling. Shewanella oneidensis H-NOX (So H-NOX) is associated with a histidine kinase (So HnoK) encoded on the same operon, and together they form a multicomponent signaling network whereby the NO-bound state of So H-NOX inhibits So HnoK autophosphorylation activity, affecting the phosphorylation state of three response regulators. Although the conformational changes of So H-NOX upon NO binding have been structurally characterized, the mechanism of HnoK inhibition by NO-bound So H-NOX remains unclear...
February 21, 2017: Biochemistry
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