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Histidine kinase

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https://www.readbyqxmd.com/read/28545064/transcriptomic-analysis-of-the-response-of-pseudomonas-fluorescens-to-epigallocatechin-gallate-by-rna-seq
#1
Xiaoxiang Liu, Bimiao Shen, Peng Du, Nan Wang, Jiaxue Wang, Jianrong Li, Aihua Sun
Epigallocatechin gallate (EGCG) is a main constituent of green tea polyphenols that are widely used as food preservatives and are considered to be safe for consumption. However, the underlying antimicrobial mechanism of EGCG and the bacterial response to EGCG are not clearly understood. In the present study, a genome-wide transcriptional analysis of a typical spoilage bacterium, Pseudomonas fluorescens that responded to EGCG was performed using RNA-seq technology. A total of 26,365,414 and 23,287,092 clean reads were generated from P...
2017: PloS One
https://www.readbyqxmd.com/read/28544098/crystal-structure-of-the-sensor-domain-of-baes-from-serratia-marcescens-fs14
#2
Ya Zhang, Shenshen Qiu, Shanshan Jia, Dongqing Xu, Tingting Ran, Weiwu Wang
The sensor histidine kinases of two-component signal-transduction systems (TCSs) are essential for bacteria to adapt to variable environmental conditions. The two-component regulatory system BaeS/R increases multidrug and metal resistance in Salmonella and Escherichia coli. In this study, we report the X-ray structure of the periplasmic sensor domain of BaeS from Serratia marcescens FS14. The BaeS sensor domain (34-160) adopts a mixed α/β-fold containing a central four-stranded antiparallel β-sheet flanked by a long N-terminal α-helix and additional loops and a short C-terminal α-helix on each side...
May 24, 2017: Proteins
https://www.readbyqxmd.com/read/28542616/regulation-of-acetyl-coa-synthetase-transcription-by-the-crbs-r-two-component-system-is-conserved-in-genetically-diverse-environmental-pathogens
#3
Kristin Jacob, Anna Rasmussen, Paul Tyler, Mariah M Servos, Mariame Sylla, Cecilia Prado, Elizabeth Daniele, Josh S Sharp, Alexandra E Purdy
The CrbS/R two-component signal transduction system is a conserved regulatory mechanism through which specific Gram-negative bacteria control acetate flux into primary metabolic pathways. CrbS/R governs expression of acetyl-CoA synthase (acsA), an enzyme that converts acetate to acetyl-CoA, a metabolite at the nexus of the cell's most important energy-harvesting and biosynthetic reactions. During infection, bacteria can utilize this system to hijack host acetate metabolism and alter the course of colonization and pathogenesis...
2017: PloS One
https://www.readbyqxmd.com/read/28533471/the-atypical-response-regulator-atvr-is-a-new-player-in-pseudomonas-aeruginosa-response-to-hypoxia-and-virulence
#4
Gilberto Hideo Kaihami, Leandro Carvalho Dantas Breda, José Roberto Fogaça de Almeida, Thays de Oliveira Pereira, Gianlucca Gonçalves Nicastro, Ana Laura Boechat, Sandro Rogério de Almeida, Regina Lúcia Baldini
Two-component systems are widespread in bacteria, allowing adaptation to environmental changes. The classical pathway is composed by a histidine kinase that phosphorylates an aspartate residue in the cognate response regulator (RR). RRs lacking the phosphorylable aspartate also occur, but their function and contribution during host-pathogen interaction are poorly characterized. AtvR (PA14_26570) is the only atypical response regulator with a DNA-binding domain in the opportunistic pathogen Pseudomonas aeruginosa Macrophage infection with the atvR mutant strain resulted in higher TNF-α secretion as well as increased bacterial clearance as compared to macrophages infected with the wild-type strain...
May 22, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28533218/involvement-of-two-component-signaling-in-bacterial-motility-and-biofilm-development
#5
Birgit M Prüβ
Two-component signaling is a specialized mechanism that bacteria use to respond to changes in their environment. Non-pathogenic strains of E. coli K-12 harbor 30 histidine kinases and 32 response regulators, which form a network of regulation that integrates many other global regulators that do not follow the two-component signaling mechanism, as well as signals from central metabolism. The output of this network is a multitude of phenotypic changes in response to changes in the environment. Among these phenotypic changes, many two-component systems control motility and/or the formation of biofilm, sessile communities of bacteria that form on surfaces...
May 22, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28527583/molecular-characterization-of-aspergillus-fumigatus-tcsc-a-characteristic-type-iii-hybrid-histidine-kinase-of-filamentous-fungi-harboring-six-hamp-domains
#6
Anja Spadinger, Frank Ebel
The type III hybrid histidine kinase (HHK) TcsC enables the pathogenic mold Aspergillus fumigatus to thrive under hyperosmotic conditions. It is, moreover, of particular interest, since it is the target of certain antifungal agents, such as fludioxonil. This study was aimed at a functional characterization of the domains that constitute the sensing and the kinase module of TcsC. The sensing module consists of six HAMP domains, an architecture that is commonly found in type III HHKs of filamentous fungi. To dissect the functional role of the individual domains, we have analyzed a set of truncated derivatives of TcsC with respect to their impact on fungal growth and their ability to respond to hyperosmotic stress and fludioxonil...
May 11, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28522691/mechanism-of-transmembrane-signaling-by-sensor-histidine-kinases
#7
Ivan Gushchin, Igor Melnikov, Vitaly Polovinkin, Andrii Ishchenko, Anastasia Yuzhakova, Pavel Buslaev, Gleb Bourenkov, Sergei Grudinin, Ekaterina Round, Taras Balandin, Valentin Borshchevskiy, Dieter Willbold, Gordon Leonard, Georg Büldt, Alexander Popov, Valentin Gordeliy
One of the major and essential classes of transmembrane (TM) receptors, present in all domains of life, is sensor histidine kinases (HKs), parts of two-component signaling systems (TCS). The structural mechanisms of transmembrane signaling by these sensors are poorly understood. We present here crystal structures of the periplasmic sensor domain, the TM domain and the cytoplasmic HAMP domain of the Escherichia coli nitrate/nitrite sensor HK NarQ in the ligand-bound and mutated ligand-free states. The structures reveal that the ligand binding induces significant rearrangements and piston-like shifts of TM helices...
May 18, 2017: Science
https://www.readbyqxmd.com/read/28512092/quantitative-kinetic-analyses-of-shutting-off-a-two-component-system
#8
Rong Gao, Ann M Stock
Cells rely on accurate control of signaling systems to adapt to environmental perturbations. System deactivation upon stimulus removal is as important as activation of signaling pathways. The two-component system (TCS) is one of the major bacterial signaling schemes. In many TCSs, phosphatase activity of the histidine kinase (HK) is believed to play an essential role in shutting off the pathway and resetting the system to the prestimulus state. Two basic challenges are to understand the dynamic behavior of system deactivation and to quantitatively evaluate the role of phosphatase activity under natural cellular conditions...
May 16, 2017: MBio
https://www.readbyqxmd.com/read/28511809/characterisation-of-the-selective-binding-of-antibiotics-vancomycin-and-teicoplanin-by-the-vans-receptor-regulating-type-a-vancomycin-resistance-in-the-enterococci
#9
C S Hughes, E Longo, M K Phillips-Jones, R Hussain
A-type resistance towards "last-line" glycopeptide antibiotic vancomycin in the leading hospital acquired infectious agent, the enterococci, is the most common in the UK. Resistance is regulated by the VanRASA two-component system, comprising the histidine sensor kinase VanSA and the partner response regulator VanRA. The nature of the activating ligand for VanSA has not been identified, therefore this work sought to identify and characterise ligand(s) for VanSA. In vitro approaches were used to screen the structural and activity effects of a range of potential ligands with purified VanSA protein...
May 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28500045/sodium-lactate-negatively-regulates-biofilm-formation-of-shewanella-putrefaciens-cn32-via-a-three-component-regulatory-system-lrbs-a-r
#10
Cong Liu, Jinshui Yang, Liang Liu, Baozhen Li, Hongli Yuan, Weijie Liu
The capability of biofilm formation has a major impact on the industrial and biotechnological applications of Shewanella putrefaciens CN32. However, the detailed regulatory mechanisms underlying biofilm formation in S. putrefaciens CN32 remain largely unknown. In the present study, we describe a three-component regulatory system which negatively regulates the biofilm formation of S. putrefaciens CN32. The three-component regulatory system consists of a histidine kinase LrbS (Sputcn32_0303) and two cognate response regulators including a transcription factor LrbA (Sputcn32_0304) and a phosphodiesterase LrbR (Sputcn32_0305)...
May 12, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28500030/translating-divergent-environmental-stresses-into-a-common-proteome-response-through-hik33-in-a-model-cyanobacterium
#11
Haitao Ge, Longfa Fang, Xiahe Huang, Jinlong Wang, Weiyang Chen, Ye Liu, Yuanya Zhang, Xiaorong Wang, Wu Xu, Qingfang He, Yingchun Wang
The histidine kinase Hik33 plays important roles in mediating cyanobacterial response to divergent types of abiotic stresses including cold, salt, high light (HL), and osmotic stresses. However, how these functions are regulated by Hik33 remains to be addressed. Using a hik33-deficient strain (Δhik33) of Synechocystis sp. PCC 6803 (Synechocystis) and quantitative proteomics, we found that Hik33 depletion induces differential protein expression highly similar to that induced by divergent types of stresses. This typically includes downregulation of proteins in photosynthesis and carbon assimilation that are necessary for cell propagation, and upregulation of heat shock proteins, chaperons, and proteases that are important for cell survival...
May 12, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28499956/group-x-hybrid-histidine-kinase-chk1-is-dispensable-for-stress-adaptation-host-pathogen-interactions-and-virulence-in-the-opportunistic-yeast-candida-guilliermondii
#12
María J Navarro-Arias, Karine Dementhon, Tatiana A Defosse, Emilien Foureau, Vincent Courdavault, Marc Clastre, Solène Le Gal, Gilles Nevez, Yohann Le Govic, Jean-Philippe Bouchara, Nathalie Giglioli-Guivarc'h, Thierry Noël, Hector M Mora-Montes, Nicolas Papon
Hybrid histidine kinases (HHKs) progressively emerge as prominent sensing proteins in the fungal kingdom and as ideal targets for future therapeutics. The group X HHK is of major interest, since it was demonstrated to play an important role in stress adaptation, host-pathogen interactions and virulence in some yeast and mold models, and particularly Chk1, that corresponds to the sole group X HHK in Candida albicans. In the present work, we investigated the role of Chk1 in the low-virulence species Candida guilliermondii, in order to gain insight into putative conservation of the role of group X HHK in opportunistic yeasts...
May 9, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28484044/class-iii-histidine-kinases-a-recently-accessorized-kinase-domain-in-putative-modulators-of-type-iv-pili-based-motility
#13
Ogun Adebali, Marharyta G Petukh, Alexander O Reznik, Artem V Tishkov, Amit A Upadhyay, Igor B Zhulin
Histidine kinases are key components of regulatory systems that enable bacteria to respond to environmental changes. Two major classes of histidine kinases are recognized on the basis of their modular design: classical (HKI) and chemotaxis-specific (HKII). Recently, a new type of histidine kinases that appeared to have features of both HKI and HKII was identified and termed HKIII; however, the details of HKIII's relationship to other two classes of histidine kinases, their function and evolutionary history remain unknown...
May 8, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28483958/new-aspects-of-the-interplay-between-penicillin-binding-proteins-murm-and-the-two-component-system-ciarh-of-penicillin-resistant-streptococcus-pneumoniae-serotype-19a-isolates-from-hungary
#14
Inga Schweizer, Sebastian Blättner, Patrick Maurer, Katharina Peters, Daniela Vollmer, Waldemar Vollmer, Regine Hakenbeck, Dalia Denapaite
The Streptococcus pneumoniae clone Hungary(19A)-6 can expresses unusually high levels of β-lactam resistance which is in part due to mutations in the MurM gene encoding a transferase involved in the synthesis of branched peptidoglycan. Moreover it contains the allele ciaH232 encoding the histidine kinase CiaH (M Müller, P Marx, R Hakenbeck, and R Brückner, Microbiology 157:3104-12, 2011, doi: 10.1099/mic.0.053157-0). High-level penicillin resistance primarily requires the presence of low-affinity (mosaic) PBP genes as, for example, in strain Hu17, a closely related member of the Hungary(19A)-6 lineage...
May 8, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28475313/signal-sensing-and-transduction-by-histidine-kinases-as-unveiled-through-studies-on-a-temperature-sensor
#15
Luciano A Abriata, Daniela Albanesi, Matteo Dal Peraro, Diego de Mendoza
Histidine kinases (HK) are the sensory proteins of two-component systems, responsible for a large fraction of bacterial responses to stimuli and environmental changes. Prototypical HKs are membrane-bound proteins that phosphorylate cognate response regulator proteins in the cytoplasm upon signal detection in the membrane or periplasm. HKs stand as potential drug targets but also constitute fascinating systems for studying proteins at work, specifically regarding the chemistry and mechanics of signal detection, transduction through the membrane, and regulation of catalytic outputs...
May 5, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28472718/cyanobacteria-in-motion
#16
REVIEW
Nils Schuergers, Conrad W Mullineaux, Annegret Wilde
Cyanobacteria are able to move directly towards or away from a light source, a process called phototaxis. Recent studies have revealed that the spherical unicellular cyanobacterium Synechocystis sp. PCC 6803 exhibits a cell polarity in response to unidirectional illumination and that micro-optic properties of cyanobacterial cells are the basis of their directional light sensing. Further functional and physiological studies highlight a very complex control of cyanobacterial phototaxis by sensory proteins, histidine kinases and response regulators...
May 1, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28470426/the-yeasts-phosphorelay-systems-a-comparative-view
#17
REVIEW
Griselda Salas-Delgado, Laura Ongay-Larios, Laura Kawasaki-Watanabe, Imelda López-Villaseñor, Roberto Coria
Cells contain signal transduction pathways that mediate communication between the extracellular environment and the cell interior. These pathways control transcriptional programs and posttranscriptional processes that modify cell metabolism in order to maintain homeostasis. One type of these signal transduction systems are the so-called Two Component Systems (TCS), which conduct the transfer of phosphate groups between specific and conserved histidine and aspartate residues present in at least two proteins; the first protein is a sensor kinase which autophosphorylates a histidine residue in response to a stimulus, this phosphate is then transferred to an aspartic residue located in a response regulator protein...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28469239/identification-of-a-high-affinity-pyruvate-receptor-in-escherichia-coli
#18
Stefan Behr, Ivica Kristoficova, Michael Witting, Erin J Breland, Allison R Eberly, Corinna Sachs, Philippe Schmitt-Kopplin, Maria Hadjifrangiskou, Kirsten Jung
Two-component systems are crucial for signal perception and modulation of bacterial behavior. Nevertheless, to date, very few ligands have been identified that directly interact with histidine kinases. The histidine kinase/response regulator system YehU/YehT of Escherichia coli is part of a nutrient-sensing network. Here we demonstrate that this system senses the onset of nutrient limitation in amino acid rich media and responds to extracellular pyruvate. Binding of radiolabeled pyruvate was found for full-length YehU in right-side-out membrane vesicles as well as for a truncated, membrane-integrated variant, confirming that YehU is a high-affinity receptor for extracellular pyruvate...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28469162/blue-light-reception-through-quaternary-transitions
#19
Christopher Engelhard, Ralph P Diensthuber, Andreas Möglich, Robert Bittl
Sensory photoreceptors absorb light via their photosensor modules and trigger downstream physiological adaptations via their effector modules. Light reception accordingly depends on precisely orchestrated interactions between these modules, the molecular details of which often remain elusive. Using electron-electron double resonance (ELDOR) spectroscopy and site-directed spin labelling, we chart the structural transitions facilitating blue-light reception in the engineered light-oxygen-voltage (LOV) histidine kinase YF1 which represents a paradigm for numerous natural signal receptors...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28461451/a-novel-two-component-system-glur-k-involved-in-glutamate-sensing-and-uptake-in-streptomyces-coelicolor
#20
Lei Li, Weihong Jiang, Yinhua Lu
Two-component systems (TCSs), the predominant signal transduction pathways employed by bacteria, play important roles in physiological metabolism in Streptomyces Here, a novel TCS GluR-K (encoded by SCO5778-5779), which is located divergently from the gluABCD operon encoding a glutamate uptake system, was identified being involved in glutamate sensing and uptake as well as antibiotic biosynthesis in Streptomyces coelicolor Under the minimal medium (MM) supplemented with different concentrations of glutamate, deletion of gluR-K resulted in enhanced ACT but reduced RED and yCPK production, suggesting GluR-K plays a differential role in antibiotic biosynthesis...
May 1, 2017: Journal of Bacteriology
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