keyword
MENU ▼
Read by QxMD icon Read
search

Histidine kinase

keyword
https://www.readbyqxmd.com/read/27924260/molecular-mechanisms-of-substrate-specificities-of-uridinecytidine-kinase
#1
Wataru Tanaka, Mitsuo Shoji, Fumiaki Tomoike, Yuzuru Ujiie, Kyohei Hanaoka, Ryuhei Harada, Megumi Kayanuma, Katsumasa Kamiya, Toyokazu Ishida, Ryoji Masui, Seiki Kuramitsu, Yasuteru Shigeta
A uridine-cytidine kinase (UCK) catalyzes the phosphorylation of uridine (Urd) and cytidine (Cyd) and plays a significant role in the pyrimidine-nucleotide salvage pathway. Unlike ordinary ones, UCK from Thermus thermophilus HB8 (ttCK) loses catalytic activity on Urd due to lack of a substrate binding ability and possesses an unusual amino acid, i.e. tyrosine 93 (Tyr93) at the binding site, whereas histidine (His) is located in the other UCKs. Mutagenesis experiments revealed that a replacement of Tyr93 by His or glutamine (Gln) recovered catalytic activity on Urd...
2016: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/27919888/identification-of-a-novel-lincomycin-resistance-mutation-associated-with-activation-of-antibiotic-production-in-streptomyces-coelicolor-a3-2
#2
Guojun Wang, Masumi Izawa, Xiaoge Yang, Dongbo Xu, Yukinori Tanaka, Kozo Ochi
Comparative genome sequencing analysis of a lincomycin-resistant strain of Streptomyces coelicolor A3 (2) and the wild type strain identified a novel mutation conferring a high level of lincomycin resistance. Surprisingly, the new mutation was an in-frame DNA deletion in the genes SCO4597-SCO4598, resulting in formation of the hybrid gene linR SCO4597 and SCO4598 encode two histidine kinases, which, together with SCO4596, encoding a response regulator, constitute a unique two-component system. Sequence analysis indicated that these three genes and their arrangement patterns are ubiquitous among all Streptomyces genomes sequenced to date, suggesting these genes play important regulatory roles...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27903631/genomics-of-compensatory-adaptation-in-experimental-populations-of-aspergillus-nidulans
#3
Jeremy R Dettman, Nicolas Rodrigue, Sijmen E Schoustra, Rees Kassen
Knowledge of the number and nature of genetic changes responsible for adaptation is essential for understanding and predicting evolutionary trajectories. Here, we study the genomic basis of compensatory adaptation to the fitness cost of fungicide resistance in experimentally evolved strains of the filamentous fungus Aspergillus nidulans The original selection experiment tracked the fitness recovery of lines founded by an ancestral strain that was resistant to fludioxonil, but paid a fitness cost in the absence of the fungicide...
November 30, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27902439/evidence-that-pneumococcal-walk-is-regulated-by-stkp-through-protein-protein-interaction
#4
Gro Anita Stamsås, Daniel Straume, Zhian Salehian, Leiv Sigve Håvarstein
WalRK is the only two-component regulatory system essential for viability in Streptococcus pneumoniae. Despite its importance, the biological role of this system is not well understood. However, previous studies have shown that it has a crucial role in controlling pneumococcal cell division. Considerable efforts have been made to understand how the WalRK system is regulated, but no signal(s) sensed by the WalK histidine kinase has been identified so far. Here, we provide evidence that the serine/threonine protein kinase StkP modulates the activity of WalK through direct protein-protein interaction, suggesting that this interaction is one of the signals sensed by WalK...
November 29, 2016: Microbiology
https://www.readbyqxmd.com/read/27895624/visualizing-evolutionary-relationships-of-multidomain-proteins-an-example-from-receiver-rec-domains-of-sensor-histidine-kinases-in-the-candidatus-maribeggiatoa-str-orange-guaymas-draft-genome
#5
Barbara J MacGregor
For multidomain proteins, evolutionary changes may occur at the domain as well as the whole-protein level. An example is presented here, with suggestions for how such complicated relationships might be visualized. Earlier analysis of the Candidatus Maribeggiatoa str. Orange Guaymas (BOGUAY; Gammaproteobacteria) single-filament draft genome found evidence of gene exchange with the phylogenetically distant Cyanobacteria, particularly for sensory and signal transduction proteins. Because these are modular proteins, known to undergo frequent duplication, domain swapping, and horizontal gene transfer, a single domain was chosen for analysis...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27883080/construction-of-a-miniaturized-chromatic-acclimation-sensor-from-cyanobacteria-with-reversed-response-to-a-light-signal
#6
Mitsuharu Nakajima, Stefano Ferri, Matthias Rögner, Koji Sode
Cyanobacteria harbor unique photoreceptors, designated as cyanobacteriochromes (CBCRs). In this study, we attempted to engineer the chromatic acclimation sensor CcaS, a CBCR derived from the cyanobacterium Synechocystis sp. PCC 6803. The wild-type CcaS induces gene expression under green light illumination and represses it under red light illumination. We focused on the domain structure of CcaS, which consists of an N-terminal transmembrane helix; a GAF domain, which serves as the sensor domain; a linker region (L1); two PAS domains; a second linker region (L2); and a C-terminal histidine kinase (HK) domain...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27877157/cross-talk-between-staphylococcus-aureus-and-other-staphylococcal-species-via-the-agr-quorum-sensing-system
#7
Jaime Canovas, Mara Baldry, Martin S Bojer, Paal S Andersen, Piotr K Grzeskowiak, Marc Stegger, Peter Damborg, Christian A Olsen, Hanne Ingmer
Staphylococci are associated with both humans and animals. While most are non-pathogenic colonizers, Staphylococcus aureus is an opportunistic pathogen capable of causing severe infections. S. aureus virulence is controlled by the agr quorum sensing system responding to secreted auto-inducing peptides (AIPs) sensed by AgrC, a two component histidine kinase. agr loci are found also in other staphylococcal species and for Staphylococcus epidermidis, the encoded AIP represses expression of agr regulated virulence genes in S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27873257/bacterial-histidine-kinases-overexpression-purification-and-inhibitor-screen
#8
Mike Gajdiss, Michael Türck, Gabriele Bierbaum
Bacterial histidine kinases are promising targets for new antimicrobial agents. In antibacterial therapy such agents could inhibit bacterial growth by targeting essential two-component regulatory systems or resensitize bacteria to known antibiotics by blocking stress responses like the cell wall stress response. However, (1) activity assays using the truncated phosphorylation domains have been shown to produce artifacts and (2) the purification of the full-length histidine kinases is complicated. Here, we describe a standard protocol for the recombinant expression and purification of functional full-length histidine kinases and other membrane proteins from gram-positive bacteria that do not harbor more than two trans-membrane domains using an Escherichia coli host...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27872062/fludioxonil-induces-drk1-a-fungal-group-iii-hybrid-histidine-kinase-to-dephosphorylate-its-downstream-target-ypd1
#9
Stephanie M Lawry, Brad Tebbets, Iain Kean, Douglas Stewart, Joel Hetelle, Bruce S Klein
Novel anti-fungal drugs and targets are urgently needed. Group III hybrid histidine kinases (HHKs) represent an appealing new therapeutic drug target because they are widely expressed in fungi but absent in humans. We investigated the mode of action of the widely utilized, effective fungicide fludioxonil. The drug acts in an HHK-dependent manner by constitutive activation of the HOG (high osmolarity glycerol) pathway, but its mechanism of action is poorly understood. Herein, we report a new mode of drug action that entails conversion of the HHK from a kinase into a phosphatase...
November 21, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27853287/pyroptosis-of-salmonella-typhimurium-infected-macrophages-was-suppressed-and-elimination-of-intracellular-bacteria-from-macrophages-was-promoted-by-blocking-qsec
#10
Zhi Li, Qing Zheng, Xiaoyan Xue, Xin Shi, Ying Zhou, Fei Da, Di Qu, Zheng Hou, Xiaoxing Luo
QseC is a membrane-bound histidine sensor kinase found in Gram-negative pathogens and is involved in the regulation of bacterial virulence. LED209, a QseC-specific inhibitor, significantly inhibits the virulence of several pathogens and partially protects infected mice from death by blocking QseC. However, the mechanism of its antibacterial effects remains unclear. In this experiment, a Salmonella Typhimurium (S. Typhimurium) and macrophage co-culture system was utilized to investigate possible mechanisms underlying the antimicrobial effects of the QseC inhibitor...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27852620/cyclic-di-gmp-binding-induces-structural-rearrangements-in-the-plza-and-plzc-proteins-of-the-lyme-disease-and-relapsing-fever-spirochetes-a-possible-switch-mechanism-for-c-di-gmp-mediated-effector-functions
#11
Katherine L Mallory, Daniel P Miller, Lee D Oliver, John C Freedman, Jessica L Kostick-Dunn, Jason A Carlyon, James D Marion, Jessica K Bell, Richard T Marconi
The c-di-GMP network of Borrelia burgdorferi, a causative agent of Lyme disease, consists of Rrp1, a diguanylate cyclase/response regulator; Hpk1, a histidine kinase; PdeA and PdeB, c-di-GMP phosphodiesterases; and PlzA, a PilZ domain c-di-GMP receptor. Borrelia hermsii, a causative agent of tick-borne relapsing fever, possesses a putative c-di-GMP regulatory network that is uncharacterized. While B. burgdorferi requires c-di-GMP to survive within ticks, the associated effector mechanisms are poorly defined...
November 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27818655/prokaryotic-responses-to-ammonium-and-organic-carbon-reveal-alternative-co2-fixation-pathways-and-importance-of-alkaline-phosphatase-in-the-mesopelagic-north-atlantic
#12
Federico Baltar, Daniel Lundin, Joakim Palovaara, Itziar Lekunberri, Thomas Reinthaler, Gerhard J Herndl, Jarone Pinhassi
To decipher the response of mesopelagic prokaryotic communities to input of nutrients, we tracked changes in prokaryotic abundance, extracellular enzymatic activities, heterotrophic production, dark dissolved inorganic carbon (DIC) fixation, community composition (16S rRNA sequencing) and community gene expression (metatranscriptomics) in 3 microcosm experiments with water from the mesopelagic North Atlantic. Responses in 3 different treatments amended with thiosulfate, ammonium or organic matter (i.e., pyruvate plus acetate) were compared to unamended controls...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27802580/use-of-restrained-molecular-dynamics-to-predict-the-conformations-of-phosphorylated-receiver-domains-in-two-component-signaling-systems
#13
Clay A Foster, Ann H West
Two-component signaling (TCS) is the primary means by which bacteria, as well as certain plants and fungi, respond to external stimuli. Signal transduction involves stimulus-dependent autophosphorylation of a sensor histidine kinase and phosphoryl transfer to the receiver domain of a downstream response regulator. Phosphorylation acts as an allosteric switch, inducing structural and functional changes in the pathway's components. Due to their transient nature, phosphorylated receiver domains are challenging to characterize structurally...
November 1, 2016: Proteins
https://www.readbyqxmd.com/read/27799551/degradation-of-cyclic-diguanosine-monophosphate-by-a-hybrid-two-component-protein-protects-azoarcus-sp-strain-cib-from-toluene-toxicity
#14
Zaira Martín-Moldes, Blas Blázquez, Claudine Baraquet, Caroline S Harwood, María T Zamarro, Eduardo Díaz
Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger that controls diverse functions in bacteria, including transitions from planktonic to biofilm lifestyles, virulence, motility, and cell cycle. Here we describe TolR, a hybrid two-component system (HTCS), from the β-proteobacterium Azoarcus sp. strain CIB that degrades c-di-GMP in response to aromatic hydrocarbons, including toluene. This response protects cells from toluene toxicity during anaerobic growth. Whereas wild-type cells tolerated a sudden exposure to a toxic concentration of toluene, a tolR mutant strain or a strain overexpressing a diguanylate cyclase gene lost viability upon toluene shock...
November 15, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27775685/lads-is-a-calcium-responsive-kinase-that-induces-acute-to-chronic-virulence-switch-in-pseudomonas-aeruginosa
#15
Ursula N Broder, Tina Jaeger, Urs Jenal
Virulence of pathogenic bacteria is a tightly controlled process to facilitate invasion and survival in host tissues. Although pathways controlling virulence have been defined in detail, signals modulating these processes are poorly understood. The opportunistic pathogen Pseudomonas aeruginosa causes acute and chronic infections in humans. Disease progression is typically associated with a loss of acute virulence and the emergence of biofilms and chronic behaviour. The acute-to-chronic switch is governed by the global Gac/Rsm pathway...
October 24, 2016: Nature Microbiology
https://www.readbyqxmd.com/read/27774581/a-preliminary-study-of-new-single-polymorphisms-in-th17-pathway-for-psoriasis-in-the-korean-population
#16
Soo Young Kim, Min Seok Hur, Byung Gon Choi, Min Jung Kim, Yang Won Lee, Yong Beom Choe, Kyu Joong Ahn
Psoriasis is a polygenic and multifactorial disease showing ethnic differences in terms of its severity and frequency. Therapies targeting interleukin 17A (IL-17A), interleukin 17 receptor (IL-17R), and Janus kinases (JAKs) are in clinical development for the treatment of psoriasis, and their success suggests the essential role of these molecules in psoriasis. To investigate the genetic susceptibility in T helper 17 (Th17) cell signal transduction pathways for promoting psoriasis, we performed candidate gene and linkage disequilibrium analysis...
October 24, 2016: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/27752127/structural-characterization-of-the-circadian-clock-protein-complex-composed-of-kaib-and-kaic-by-inverse-contrast-matching-small-angle-neutron-scattering
#17
Masaaki Sugiyama, Hirokazu Yagi, Kentaro Ishii, Lionel Porcar, Anne Martel, Katsuaki Oyama, Masanori Noda, Yasuhiro Yunoki, Reiko Murakami, Rintaro Inoue, Nobuhiro Sato, Yojiro Oba, Kazuki Terauchi, Susumu Uchiyama, Koichi Kato
The molecular machinery of the cyanobacterial circadian clock consists of three proteins: KaiA, KaiB, and KaiC. Through interactions among the three Kai proteins, the phosphorylation states of KaiC generate circadian oscillations in vitro in the presence of ATP. Here, we characterized the complex formation between KaiB and KaiC using a phospho-mimicking mutant of KaiC, which had an aspartate substitution at the Ser431 phosphorylation site and exhibited optimal binding to KaiB. Mass-spectrometric titration data showed that the proteins formed a complex exclusively in a 6:6 stoichiometry, indicating that KaiB bound to the KaiC hexamer with strong positive cooperativity...
October 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27720925/broadening-the-antibacterial-spectrum-of-histidine-kinase-autophosphorylation-inhibitors-via-the-use-of-%C3%AE%C2%B5-poly-l-lysine-capped-mesoporous-silica-based-nanoparticles
#18
Nadya Velikova, Nuria Mas, Laura Miguel-Romero, Lorena Polo Aguado, Ellen Stolte, Edoardo Zaccaria, Rui Cao, Nico Taverne, José Ramón Murguía, Ramon Martinez-Manez, Alberto Marina, Jerry Wells
Two-component systems (TCS) regulate diverse processes such as virulence, stress responses, metabolism and antibiotic resistance in bacteria but are absent in humans, making them promising targets for novel antibacterials. By incorporating recently described TCS histidine kinase autophosphorylation inhibitors (HKAIs) into ε-poly-L-lysine capped nanoparticles (NPs) we could overcome the Gram negative (Gr-) permeability barrier for the HKAIs. The observed bactericidal activity against Gr- bacteria was shown to be due to the enhanced delivery and internalization of the HKAIs and not an inhibitory or synergistic effect of the NPs...
October 5, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27716889/establishment-of-two-data-mining-models-of-lung-cancer-screening-based-on-three-gene-promoter-methylations-combined-with-telomere-damage
#19
Wei Wang, Xiaolei Feng, Xiaoran Duan, Shanjuan Tan, Sihua Wang, Tuanwei Wang, Feifei Feng, Yiming Wu, Yongjun Wu
OBJECTIVE: To identify the significance of a support vector machine (SVM) model and a decision tree (DT) model for the diagnosis of lung cancer combined with the detection of fragile histidine triad (FHIT), RAS association domain family 1 (RASSF1A) and cyclin-dependent kinase inhibitor 2A (p16) promoter methylation levels and relative telomere length (RTL) of white blood cells from peripheral blood DNA. METHODS: The levels of p16, RASSF1A and FHIT promoter methylation and the RTL of white blood cells in peripheral blood DNA of 200 healthy individuals and 200 lung cancer patients were analyzed by SYBR Green-based quantitative methylation-specific PCR and quantitative PCR...
September 24, 2016: International Journal of Biological Markers
https://www.readbyqxmd.com/read/27714507/nmr-assignments-of-the-gacs-histidine-kinase-periplasmic-detection-domain-from-pseudomonas-aeruginosa-pao1
#20
Ahmad Ali-Ahmad, Olivier Bornet, Firas Fadel, Yves Bourne, Florence Vincent, Christophe Bordi, Françoise Guerlesquin, Corinne Sebban-Kreuzer
Pseudomonas aeruginosa is a highly adaptable opportunistic pathogen. It can infect vulnerable patients such as those with cystic fibrosis or hospitalized in intensive care units where it is responsible for both acute and chronic infection. The switch between these infections is controlled by a complex regulatory system involving the central GacS/GacA two-component system that activates the production of two small non-coding RNAs. GacS is a histidine kinase harboring one periplasmic detection domain, two inner-membrane helices and three H1/D1/H2 cytoplasmic domains...
October 6, 2016: Biomolecular NMR Assignments
keyword
keyword
9101
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"