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Histidine kinase

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https://www.readbyqxmd.com/read/28819239/sequential-conformational-transitions-and-%C3%AE-helical-supercoiling-regulate-a-sensor-histidine-kinase
#1
Oskar Berntsson, Ralph P Diensthuber, Matthijs R Panman, Alexander Björling, Emil Gustavsson, Maria Hoernke, Ashley J Hughes, Léocadie Henry, Stephan Niebling, Heikki Takala, Janne A Ihalainen, Gemma Newby, Silke Kerruth, Joachim Heberle, Marianne Liebi, Andreas Menzel, Robert Henning, Irina Kosheleva, Andreas Möglich, Sebastian Westenhoff
Sensor histidine kinases are central to sensing in bacteria and in plants. They usually contain sensor, linker, and kinase modules and the structure of many of these components is known. However, it is unclear how the kinase module is structurally regulated. Here, we use nano- to millisecond time-resolved X-ray scattering to visualize the solution structural changes that occur when the light-sensitive model histidine kinase YF1 is activated by blue light. We find that the coiled coil linker and the attached histidine kinase domains undergo a left handed rotation within microseconds...
August 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28814746/rewiring-of-the-ftsh-regulatory-network-by-a-single-nucleotide-change-in-saes-of-staphylococcus-aureus
#2
Qian Liu, Mo Hu, Won-Sik Yeo, Lei He, Tianming Li, Yuanjun Zhu, Hongwei Meng, Yanan Wang, Hyunwoo Lee, Xiaoyun Liu, Min Li, Taeok Bae
In the Gram-positive pathogen Staphylococcus aureus, the membrane-bound ATP-dependent metalloprotease FtsH plays a critical role in resistance to various stressors. However, the molecular mechanism of the FtsH functions is not known. Here, we identified core FtsH target proteins in S. aureus. In the strains Newman and USA300, the abundance of 33 proteins were altered in both strains, of which 11 were identified as core FtsH substrate protein candidates. In the strain Newman and some other S. aureus strains, the sensor histidine kinase SaeS has an L18P (T53C in saeS) substitution, which transformed the protein into an FtsH substrate...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28812258/chromosomal-flhb1-gene-of-the-alphaproteobacterium-azospirillum-brasilense-sp245-is-essential-for-correct-assembly-of-both-constitutive-polar-flagellum-and-inducible-lateral-flagella
#3
Yulia Filip'echeva, Andrei Shelud'ko, Alexei Prilipov, Elizaveta Telesheva, Dmitry Mokeev, Andrei Burov, Lilia Petrova, Elena Katsy
Azospirillum brasilense has the ability of swimming and swarming motility owing to the work of a constitutive polar flagellum and inducible lateral flagella, respectively. The interplay between these flagellar systems is poorly understood. One of the key elements of the flagellar export apparatus is the protein FlhB. Two predicted flhB genes are present in the genome of A. brasilense Sp245 (accession nos. HE577327-HE577333). Experimental evidence obtained here indicates that the chromosomal coding sequence (CDS) AZOBR_150177 (flhB1) of Sp245 is essential for the production of both types of flagella...
August 15, 2017: Folia Microbiologica
https://www.readbyqxmd.com/read/28802267/development-of-a-transient-muts-based-hypermutation-system-for-adaptive-evolution-of-lactobacillus-casei-to-low-ph
#4
Tom J Overbeck, Dennis L Welker, Joanne E Hughes, James L Steele, Jeff R Broadbent
This study explored transient inactivation of the gene encoding the DNA mismatch repair enzyme MutS as a tool for adaptive evolution of Lactobacillus casei MutS deletion derivatives of L. casei 12A and ATCC 334 were constructed and subject to a 100-day adaptive evolution process to increase lactic acid resistance at low pH. Wild-type parental strains were also subject to this treatment. At the end of the process, the ΔmutS lesion was repaired in representative L. casei 12A and ATCC 334 ΔmutS isolates. Growth studies in broth at pH 4...
August 11, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28796832/comparative-analysis-of-lyts-lyttr-type-histidine-kinase-response-regulator-systems-in-%C3%AE-proteobacteria
#5
Stefan Behr, Sophie Brameyer, Michael Witting, Philipp Schmitt-Kopplin, Kirsten Jung
Bacterial histidine kinase/response regulator systems operate at the interface between environmental cues and physiological states. Escherichia coli contains two LytS/LytTR-type histidine kinase/response regulator systems, BtsS/BtsR (formerly YehU/YehT) and YpdA/YpdB, which have been identified as pyruvate-responsive two-component systems. Since they exhibit remarkable similarity, we analyzed their phylogenetic distribution within the γ-proteobacteria, and experimentally characterized them in a set of representative species...
2017: PloS One
https://www.readbyqxmd.com/read/28782049/asymmetric-structure-of-the-dimerization-domain-of-phor-a-sensor-kinase-important-for-the-virulence-of-mycobacterium-tuberculosis
#6
Daniel Xing, Michelle B Ryndak, Liqin Wang, Irina Kolesnikova, Issar Smith, Shuishu Wang
The PhoP-PhoR two-component system is essential for the virulence of Mycobacterium tuberculosis (Mtb) and therefore represents a potential target for developing novel antituberculosis therapies. However, little is known about the mechanism by which this two-component system regulates the virulence. In this study, we demonstrated that a phoR mutant Mtb strain has phenotypes similar to those of a phoP mutant, suggesting that PhoP and PhoR work in the same pathway to regulate Mtb virulence. We determined the structure of the dimerization and histidine phosphotransfer (DHp) domain of PhoR to a 1...
July 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/28758290/crystal-structure-of-master-biofilm-regulator-csgd-regulatory-domain-reveals-an-atypical-receiver-domain
#7
Yurong Wen, Zhenlin Ouyang, Bart Devreese, Wangxiao He, Yongping Shao, Wuyuan Lu, Fang Zheng
The master regulator CsgD switches planktonic growth to biofilm formation by activating synthesis of curli fimbriae and cellulose in Enterobacteriaceae. CsgD was classified to be the LuxR response regulatory family, while its cognate sensor histidine kinase has not been identified yet. CsgD consists of a C-terminal DNA binding domain and an N-terminal regulatory domain that provokes the upstream signal transduction to further modulate its function. We provide the crystal structure of Salmonella Typhimurium CsgD regulatory domain, which reveals an atypical β5α5 response regulatory receiver domain folding with the α2 helix representing as a disorder loop compared to the LuxR/FixJ canonical response regulator, and the structure indicated a noteworthy α5 helix similar to the non-canonical master regulator VpsT receiver domain α6...
July 30, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28752667/genetic-networks-controlled-by-the-bacterial-replication-initiator-and-transcription-factor-dnaa-in-bacillus-subtilis
#8
Tracy A Washington, Janet L Smith, Alan D Grossman
DnaA is the widely conserved bacterial AAA+ ATPase that functions as both the replication initiator and a transcription factor. In many organisms, DnaA controls expression of its own gene and likely several others during growth and in response to replication stress. To evaluate the effects of DnaA on gene expression, separate from its role in replication initiation, we analyzed changes in mRNA levels in Bacillus subtilis cells with and without dnaA, using engineered strains in which dnaA is not essential. We found that dnaA was required for many of the changes in gene expression in response to replication stress...
July 27, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28750036/generation-and-phenotypic-characterization-of-pde1a-mutant-mice
#9
Xiaofang Wang, Satsuki Yamada, Wells B LaRiviere, Hong Ye, Jason L Bakeberg, María V Irazabal, Fouad T Chebib, Jan van Deursen, Peter C Harris, Caroline R Sussman, Atta Behfar, Christopher J Ward, Vicente E Torres
It has been proposed that a reduction in intracellular calcium causes an increase in intracellular cAMP and PKA activity through stimulation of calcium inhibitable adenylyl cyclase 6 and inhibition of phosphodiesterase 1 (PDE1), the main enzymes generating and degrading cAMP in the distal nephron and collecting duct, thus contributing to the development and progression of autosomal dominant polycystic kidney disease (ADPKD). In zebrafish pde1a depletion aggravates and overexpression ameliorates the cystic phenotype...
2017: PloS One
https://www.readbyqxmd.com/read/28746737/divergent-expression-of-cytokinin-biosynthesis-signaling-and-catabolism-genes-underlying-differences-in-feeding-sites-induced-by-cyst-and-root-knot-nematodes
#10
Carola D Dowd, Demosthenis Chronis, Zoran S Radakovic, Shahid Siddique, Thomas Schmülling, Tomáš Werner, Tatsuo Kakimoto, Florian M W Grundler, Melissa G Mitchum
Cyst and root-knot nematodes are obligate parasites of economic importance with a remarkable ability to reprogram root cells into unique metabolically active feeding sites. Previous studies have suggested a role for cytokinin in feeding site formation induced by these two types of nematodes, but the mechanistic details have not yet been described. Using Arabidopsis as a host plant species, we conducted a comparative analysis of cytokinin genes in response to the beet cyst nematode (BCN), Heterodera schachtii, and the root-knot nematode (RKN), Meloidogyne incognita...
July 26, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28743208/bacterial-signal-transduction-networks-via-connectors-and-development-of-the-inhibitors-as-alternative-antibiotics
#11
Ryutaro Utsumi
Bacterial cells possess a signal transduction system that differs from those described in higher organisms, including human cells. These so-called two-component signal transduction systems (TCSs) consist of a sensor (histidine kinase, HK) and a response regulator, and are involved in cellular functions, such as virulence, drug resistance, biofilm formation, cell wall synthesis, cell division. They are conserved in bacteria across all species. Although TCSs are often studied and characterized individually, they are assumed to interact with each other and form signal transduction networks within the cell...
September 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/28736523/sub-minimum-inhibitory-concentrations-of-rhubarb-water-extracts-inhibit-streptococcus-suis-biofilm-formation
#12
Wen-Ya Ding, Yan-Hua Li, He Lian, Xiao-Yu Ai, Yu-Lin Zhao, Yan-Bei Yang, Qiang Han, Xin Liu, Xue-Ying Chen, Zhonggui He
Streptococcus suis is one of the most important swine pathogens, which can cause persistent infection by forming biofilms. In this study, sub-minimum inhibitory concentration (sub-MIC) of rhubarb water extracts were found to inhibit biofilm formation. Two-component signal transduction systems (TCSs), transcriptional regulators, and DNA binding proteins were compared under two conditions: (1) cells treated with sub-MIC rhubarb water extracts and (2) untreated cells. Using an isobaric tags for relative and absolute quantitation (iTRAQ) strategy, we found that TCSs constituent proteins of histidine kinase and response regulator were significantly down-regulated...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28734616/do-shoot-the-messenger-pasta-kinases-as-virulence-determinants-and-antibiotic-targets
#13
REVIEW
Daniel A Pensinger, Adam J Schaenzer, John-Demian Sauer
All domains of life utilize protein phosphorylation as a mechanism of signal transduction. In bacteria, protein phosphorylation was classically thought to be mediated exclusively by histidine kinases as part of two-component signaling systems. However, it is now well appreciated that eukaryotic-like serine/threonine kinases (eSTKs) control essential processes in bacteria. A subset of eSTKs are single-pass transmembrane proteins that have extracellular penicillin-binding-protein and serine/threonine kinase-associated (PASTA) domains which bind muropeptides...
July 19, 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/28732057/structural-basis-of-zn-ii-induced-metal-detoxification-and-antibiotic-resistance-by-histidine-kinase-czcs-in-pseudomonas-aeruginosa
#14
Dan Wang, Weizhong Chen, Shanqing Huang, Yafeng He, Xichun Liu, Qingyuan Hu, Tianbiao Wei, Hong Sang, Jianhua Gan, Hao Chen
Pseudomonas aeruginosa (P. aeruginosa) is a major opportunistic human pathogen, causing serious nosocomial infections among immunocompromised patients by multi-determinant virulence and high antibiotic resistance. The CzcR-CzcS signal transduction system in P. aeruginosa is primarily involved in metal detoxification and antibiotic resistance through co-regulating cross-resistance between Zn(II) and carbapenem antibiotics. Although the intracellular regulatory pathway is well-established, the mechanism by which extracellular sensor domain of histidine kinase (HK) CzcS responds to Zn(II) stimulus to trigger downstream signal transduction remains unclear...
July 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28716923/molecular-mechanism-of-environmental-d-xylose-perception-by-a-xylfii-lyts-complex-in-bacteria
#15
Jianxu Li, Chengyuan Wang, Gaohua Yang, Zhe Sun, Hui Guo, Kai Shao, Yang Gu, Weihong Jiang, Peng Zhang
d-xylose, the main building block of plant biomass, is a pentose sugar that can be used by bacteria as a carbon source for bio-based fuel and chemical production through fermentation. In bacteria, the first step for d-xylose metabolism is signal perception at the membrane. We previously identified a three-component system in Firmicutes bacteria comprising a membrane-associated sensor protein (XylFII), a transmembrane histidine kinase (LytS) for periplasmic d-xylose sensing, and a cytoplasmic response regulator (YesN) that activates the transcription of the target ABC transporter xylFGH genes to promote the uptake of d-xylose...
August 1, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28714556/non-canonical-activation-of-histidine-kinase-kdpd-by-phosphotransferase-protein-ptsn-through-interaction-with-the-transmitter-domain
#16
Markus Mörk-Mörkenstein, Ralf Heermann, Yvonne Göpel, Kirsten Jung, Boris Görke
The two-component system KdpD/KdpE governs K(+) homeostasis by controlling synthesis of the high affinity K(+) transporter KdpFABC. When sensing low environmental K(+) concentrations, the dimeric kinase KdpD autophosphorylates in trans and transfers the phosphoryl-group to the response regulator KdpE, which subsequently activates kdpFABC transcription. In Escherichia coli, KdpD can also be activated by interaction with the non-phosphorylated form of the accessory protein PtsN. PtsN stimulates KdpD kinase activity thereby increasing phospho-KdpE levels...
July 17, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28714256/a1s_2811-a-chea-y-like-hybrid-two-component-regulator-from-acinetobacter%C3%A2-baumannii-atcc17978-is-involved-in-surface-motility-and-biofilm-formation-in-this-bacterium
#17
Rong Chen, Ruichen Lv, Lisheng Xiao, Min Wang, Zongmin Du, Yafang Tan, Yujun Cui, Yanfeng Yan, Yanping Luo, Ruifu Yang, Yajun Song
Two-component systems in Acinetobacter baumannii are associated with its virulence, drug resistance, motility, biofilm formation, and other characteristics. In this study, we used RecAb , a genetic engineering method, to investigate the function of A1S_2811 in A. baumannii strain ATCC17978. A1S_2811, a hypothetical hybrid sensor histidine kinase/response regulator, has four histidine-containing phosphotransfer domains, a CheA-like regulatory domain, and a CheY-like receiver domain at its C terminus. Compared with the ATCC17978 strain, both surface motility and biofilm formation at the gas-liquid interface decreased significantly in the A1S_2811 knock-out strain...
July 17, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28710266/specificity-of-subtilin-mediated-activation-of-histidine-kinase-spak
#18
Christoph Geiger, Tobias Spieß, Sophie Marianne Korn, Peter Kötter, Karl-Dieter Entian
Autoinduction via two-component systems is a widespread regulatory mechanism that senses environmental and metabolic changes. Although the lantibiotics nisin and subtilin are closely related and share the same lanthionine ring structure, they autoinduce their biosynthesis in a highly specific manner. Subtilin can only activate the two-component system SpaRK of Bacillus subtilis, whereas nisin activates solely the two-component system NisRK of Lactococcus lactis To identify components that determine the specificity of subtilin autoinduction, several variants of the respective lantibiotic were analyzed for their autoinduction capacity...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28691662/role-of-the-inner-membrane-histidine-kinase-rcsc-and-outer-membrane-lipoprotein-rcsf-in-the-activation-of-the-rcs-phosphorelay-signal-transduction-system-in-escherichia-coli
#19
Takatsugu Sato, Akira Takano, Nanako Hori, Tomoko Izawa, Takayoshi Eda, Kota Sato, Mitsuru Umekawa, Hiroyoshi Miyagawa, Kenji Matsumoto, Ayako Muramatsu-Fujishiro, Kouji Matsumoto, Satoshi Matsuoka, Hiroshi Hara
The Rcs phosphorelay signal transduction system of Escherichia coli controls genes for capsule production and many other envelope-related functions and is implicated in biofilm formation. The outer-membrane lipoprotein RcsF is an essential component of the Rcs system. Mislocalization of RcsF to the periplasm or the cytoplasmic membrane leads to high activation of the Rcs system, suggesting that RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. This is consistent with the result reported by Cho et al...
July 8, 2017: Microbiology
https://www.readbyqxmd.com/read/28677348/the-pseudomonas-putida-csra-rsma-homologues-negatively-affect-c-di-gmp-pools-and-biofilm-formation-through-the-ggdef-eal-response-regulator-cfcr
#20
Óscar Huertas-Rosales, Manuel Romero, Stephan Heeb, Manuel Espinosa-Urgel, Miguel Cámara, María Isabel Ramos-González
Expression of cfcR, encoding the only GGDEF/EAL response regulator in Pseudomonas putida, is transcriptionally regulated by RpoS, ANR and FleQ, and the functionality of CfcR as a diguanylate cyclase requires the multisensor CHASE3/GAF hybrid histidine kinase named CfcA. Here an additional level of cfcR control, operating post-transcriptionally via the RNA-binding proteins RsmA, RsmE and RsmI, is unraveled. Specific binding of the three proteins to an Rsm binding motif (5'CANGGANG3') encompassing the translational start codon of cfcR was confirmed...
July 5, 2017: Environmental Microbiology
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