keyword
MENU ▼
Read by QxMD icon Read
search

Bone specific alkaline phosphatase

keyword
https://www.readbyqxmd.com/read/28737765/adrenocorticotropic-hormone-and-1-25-dihydroxyvitamin-d3-enhance-human-osteogenesis-in-vitro-by-synergistically-accelerating-the-expression-of-bone-specific-genes
#1
Irina L Tourkova, Li Liu, Nareerat Sutjarit, Quitterie C Larrouture, Jianhua Luo, Lisa J Robinson, Harry C Blair
To improve definition of the physical and hormonal support of bone formation, we studied differentiation of human osteoblasts in vitro at varying combinations of ACTH, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D), and extracellular calcium, with and without added cortisol. Bone mineralization, alkaline phosphatase activity, and osteoblast-specific markers RunX2, osterix, and collagen I increased with 10 pM ACTH, 10 nM 1,25(OH)2D, or at 2 mM calcium with important synergistic activity of combinations of any of these stimuli...
July 24, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28734779/metabolic-bone-disease-in-preterm-infants-relationship-between-radiologic-grading-in-the-wrist-and-serum-biochemical-markers
#2
S K You, J E Lee, S M Lee, H-H Cho
PURPOSE: To assess the relationship between radiographic findings of metabolic bone disease (MBD) and serum biochemical markers in preterm infants. MATERIALS AND METHODS: A total of 159 preterm infants were included in this study. Two readers reviewed the wrist radiography for grading according to MBD severity. We recorded the levels of alkaline phosphatase (ALP) and phosphorous (P) immediately after birth, on the same day of the first wrist radiography (ALP-s, P-s), the highest/lowest ALP/P levels before the first wrist radiography (ALP-hb/P-lb) and during follow-up (ALP-h/P-l)...
July 19, 2017: Diagnostic and Interventional Imaging
https://www.readbyqxmd.com/read/28731215/hypophosphatasia-in-adults-clinical-assessment-and-treatment-considerations
#3
Jay R Shapiro, E Michael Lewiecki
Hypophosphatasia (HPP) is a rare inherited disorder of bone affecting approximately 500-600 known individuals in the US. HPP is due to mutations involving the gene for tissue non-specific alkaline phosphatase. Five clinical types of HPP are recognized. The clinical presentation of HPP varies from devastating prenatal intrauterine disease to mild manifestations in adulthood. In adults, main clinical involvement includes early loss of primary or secondary teeth, osteoporosis, bone pain, chondrocalcinosis, and fractures...
July 21, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28720849/melatonin-promotes-osteoblast-differentiation-by-regulating-osterix-protein-stability-and-expression
#4
Younho Han, Young-Mi Kim, Hyung Sik Kim, Kwang Youl Lee
Although the biological role of melatonin in osteogenic differentiation has been suggested, the mechanism of osteoblast differentiation remains unclear. Thus, the present study investigated the underlying molecular mechanisms based on osteoblast-specific transcription factors. We found that melatonin enhanced BMP-4-induced osteogenic differentiation and increased the expression of osteogenic markers, especially Osterix, which is an essential transcription factor for the differentiation of preosteoblasts into mature osteoblasts in the late stage of osteoblast differentiation...
July 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28715881/comparison-of-bone-metabolism-based-on-the-different-ages-and-competition-levels-of-junior-and-high-school-female-rhythmic-gymnasts
#5
Taewoong Oh, Tatsuki Naka
PURPOSE: This study was to clarify the effect of age and competition level by measuring bone metabolism markers and bone mineral density measurements of junior high school and high school female rhythmic gymnasts, who restrict their diets to maintain a low body weight, while routinely undertaking long hours of high-intensity exercise, comparing the gymnasts based on their elite/non-elite. METHODS: The study investigated 7 junior high school and 12 high school female rhythmic gymnasts...
June 30, 2017: Journal of Exercise Nutrition & Biochemistry
https://www.readbyqxmd.com/read/28713017/intracellular-co-delivery-of-sr-ion-and-phenamil-drug-through-mesoporous-bioglass-nanocarriers-synergizes-bmp-signaling-and-tissue-mineralization
#6
Jung-Hwan Lee, Nandin Mandakhbayar, Ahmed El-Fiqi, Haw-Won Kim
Inducing differentiation and maturation of resident multipotent stem cells (MSCs) is an important strategy to regenerate hard tissues in mal-calcification conditions. Here we explore a co-delivery approach of therapeutic molecules comprised of ion and drug through a mesoporous bioglass nanoparticle (MBN) for this purpose. Recently, MBN has offered unique potential as a nanocarrier for hard tissues, in terms of high mesoporosity, bone bioactivity (and possibly degradability), tunable delivery of biomolecules, and ionic modification...
July 13, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28705683/periodontal-ligament-fibroblasts-as-a-cell-model-to-study-osteogenesis-and-osteoclastogenesis-in-fibrodysplasia-ossificans-progressiva
#7
Teun J de Vries, Ton Schoenmaker, Dimitra Micha, Jolanda Hogervorst, Siham Bouskla, Tim Forouzanfar, Gerard Pals, Coen Netelenbos, E Marelise W Eekhoff, Nathalie Bravenboer
Fibrodysplasia Ossificans Progressiva (FOP) is a progressive disease characterized by periods of heterotopic ossification of soft connective tissues, including ligaments. Though progress has been made in recent years in unraveling the underlying mechanism, patient-derived cell models are necessary to test potential treatment options. Periodontal ligament fibroblasts (PLF) from extracted teeth can be used to study deviant bone modeling processes in vitro since these cells are derived from genuine ligaments. They further provide a tool to study the hitherto unknown role of the bone morphogenesis protein receptor type 1 (BMPR-1) Activin A type 1 receptor ACVR1-R206H mutation in osteoclastogenesis...
July 10, 2017: Bone
https://www.readbyqxmd.com/read/28705682/increased-bone-matrix-mineralization-in-treatment-na%C3%A3-ve-children-with-inflammatory-bowel-disease
#8
Barbara M Misof, Paul Roschger, Klaus Klaushofer, Frank Rauch, Jinhui Ma, David R Mack, Leanne M Ward
Inflammatory bowel disease (IBD) affects many organ systems including the skeleton. In children with IBD, bone mineral density (BMD) and bone turnover are frequently low. Disturbances in bone mineralization density distribution (BMDD) are linked to alterations in bone material strength; however, BMDD has not previously been reported in children with chronic inflammatory disorders. The aim of this study was to characterize BMDD based on quantitative backscatter electron imaging in cancellous (Cn.) and cortical (Ct...
July 10, 2017: Bone
https://www.readbyqxmd.com/read/28693592/bone-metabolism-and-inflammatory-characteristics-in-14-cases-of-chronic-nonbacterial-osteomyelitis
#9
Yurika Ata, Yutaka Inaba, Hyonmin Choe, Naomi Kobayashi, Jiro Machida, Naoyuki Nakamura, Tomoyuki Saito
BACKGROUND: Chronic nonbacterial osteomyelitis (CNO) is a multifocal autoinflammatory disease that often impairs daily life in children. This study aimed to investigate the bone metabolic and inflammatory characteristics of patients with CNO, and to assess the differences between responders and nonresponders to conservative treatment. METHODS: We investigated the clinical symptoms; laboratory data including inflammatory and bone metabolic biomarkers; and imaging findings from plain radiography, magnetic resonance imaging (MRI), fluorodeoxyglucose-positron emission tomography (FDG-PET), and dual-energy x-ray absorption (DEXA) in 14 patients with CNO...
July 11, 2017: Pediatric Rheumatology Online Journal
https://www.readbyqxmd.com/read/28690911/two-year-clinical-outcome-of-denosumab-treatment-alone-and-in-combination-with-teriparatide-in-japanese-treatment-naive-postmenopausal-osteoporotic-women
#10
Yukio Nakamura, Takako Suzuki, Mikio Kamimura, Shota Ikegami, Kohei Murakami, Shigeharu Uchiyama, Akira Taguchi, Hiroyuki Kato
This randomized prospective study aimed to evaluate the clinical outcome of denosumab treatment alone and in combination with teriparatide in treatment-naive postmenopausal Japanese female patients with osteoporosis. Thirty patients were randomly assigned to two groups: (1) denosumab group (denosumab alone, n=13); and (2) combination group (denosumab+teriparatide, n=17). Serum bone-specific alkaline phosphatase (BAP), serum tartrate-resistant acid phosphatase (TRACP)-5b, urinary cross-linked N-terminal telopeptides of type I collagen (NTX), and bone mineral density (BMD) of L1-4 lumbar vertebrae (L-BMD) and bilateral total hips (H-BMD) were determined at the first visit and at various time points up to 24 months post-treatment to determine percentage changes...
2017: Bone Research
https://www.readbyqxmd.com/read/28684192/involvement-of-sphingosine-kinase-sphingosine-1-phosphate-metabolic-pathway-in-spondyloarthritis
#11
Carole Bougault, Alaeddine El Jamal, Anne Briolay, Saida Mebarek, Marie-Astrid Boutet, Thomas Garraud, Benoit Le Goff, Fréderic Blanchard, David Magne, Leyre Brizuela
Spondyloarthritis (SpA) is a relatively common chronic inflammatory joint disorder, with a prevalence of about 0.2-0.5% worldwide. The primary target of the pathological process is the enthesis, where tendons and ligaments attach to underlying bone. These insertion sites are hotspots of bone formation (enthesophytes), which can lead to ankylosis. Unfortunately, the mechanisms causing the onset and progression of entheseal ossification remain largely unknown. Sphingosine 1-phosphate (S1P), a lipid generated after sphingosine phosphorylation by sphingosine kinases 1 and 2 (SK1/2), plays important roles in cell proliferation, differentiation and survival...
July 3, 2017: Bone
https://www.readbyqxmd.com/read/28683339/risk-factors-of-metabolic-bone-disease-of-prematurity
#12
Supamit Ukarapong, Sunil Kumar Batlahally Venkatarayappa, Cristina Navarrete, Gary Berkovitz
OBJECTIVE: To identify the factors that increase risk of metabolic bone disease of prematurity (MBD). STUDY DESIGN: A retrospective case-control study of infants born between January 2013-April 2014 with gestation age <30weeks and birth weight <1000g. MBD was defined as serum alkaline phosphatase above 500U/L and characteristic radiographic changes. Information was obtained on the presence of specific comorbidities. RESULTS: Of 76 infants evaluated, 40 met criteria for MBD...
July 3, 2017: Early Human Development
https://www.readbyqxmd.com/read/28683076/cold-atmospheric-plasma-enhances-osteoblast-differentiation
#13
Kanako Tominami, Hiroyasu Kanetaka, Shota Sasaki, Takayuki Mokudai, Toshiro Kaneko, Yoshimi Niwano
This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner...
2017: PloS One
https://www.readbyqxmd.com/read/28679143/varying-patterns-of-biomarkers-of-mineral-and-bone-metabolism-after-kidney-transplantation
#14
Agnieszka Makówka, Maciej Głyda, Ewa-Rutkowska Majewska, Michał Nowicki
Sclerostin inhibits Wnt/β-catenin signaling pathway, thereby decreasing bone formation. Osteoblast stimulating actions of parathyroid hormone (PTH) are mediated by suppression of sclerostin. Thus, sclerostin may reflect both bone metabolism and parathyroid function. The study was aimed to analyze the patterns of the changes of mineral and bone biomarkers for 9 months following kidney transplantation (KTx). Thirty-five patients after KTx were included into a 9-month observational study. Serum creatinine, calcium, phosphorus, 25-OH vitamin D, PTH, fibroblast growth factor 23 (FGF-23), sclerostin, and bone-specific alkaline phosphatase (BAP) were measured before KTx, and 1, 2 weeks, and 1, 2, 3, 4, 5, 6, and 9 months thereafter...
July 5, 2017: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/28677013/a-meta-analysis-survey-of-appropriate-bone-turnover-markers-in-the-detection-of-bone-metastasis-in-lung-cancer
#15
Jianda Huang, Tongjie Gu, Jun Ying
BACKGROUND: A number of studies have investigated the clinical significance of bone turnover markers (BTMs) for the diagnosis of bone metastasis (BM) in lung cancer; however, they led to contradictory results. The aim of this meta-analysis was to investigate whether BTMs differ between lung cancer patients with and without BM. METHODS: Articles were identified by searching Medline, Embase, Web of Science and Scopus. The studies that were identified were pooled and the weighted mean difference (WMD) and its corresponding 95% confidence interval (CI) were calculated...
July 4, 2017: International Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28672891/osteogenic-potency-of-dedifferentiated-fat-cells-isolated-from-elderly-people-with-osteoporosis
#16
Wei Hao, Chuanqiang Jiang, Ming Jiang, Tian Wang, Xin Wang
Mature adipocytes are the major cell type in adipose tissue. This study aimed to explore the osteogenic potency of dedifferentiated fat cells obtained from osteoporotic patients (opDFATs) in vitro and in vivo. Mature adipocytes and adipose-derived stem cells (opASCs) were harvested from subcutaneous adipose tissue. Mature adipocytes were dedifferentiated to produce opDFATs by the ceiling culture method. OpDFATs were osteogenically induced in vitro with opASCs as a control. Cell growth, alkaline phosphatase (ALPase) activity and cell mineralization were determined, and expression levels of osteogenesis-specific genes (collagen I, osteocalcin and bone sialoprotein) were analyzed using quantitative reverse transcription polymerase chain reaction...
July 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28667771/long-term-effects-of-severe-burn-injury-on-bone-turnover-and-microarchitecture
#17
Gabriela Katharina Muschitz, Elisabeth Schwabegger, Alexandra Fochtmann, Andreas Baierl, Roland Kocijan, Judith Haschka, Wolfgang Gruther, Jakob E Schanda, Heinrich Resch, Thomas Rath, Peter Pietschmann, Christian Muschitz
Severe burn injury triggers massive alterations in stress hormone levels with a dose-dependent hypermetabolic status including increased bone resorption. This study evaluated bone microarchitecture measured by non-invasive high resolution peripheral quantitative computed tomography (HR-pQCT). Changes of serum bone turnover markers (BTM) as well as regulators of bone signaling pathways involved in skeletal health were assessed. Standardized effect sizes as a quantitative measure regarding the impact of serum changes and the prediction of these changes on bone microarchitecture were investigated...
July 1, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28662402/construction-of-an-alkaline-phosphatase-specific-two-photon-probe-and-its-imaging-application-in-living-cells-and-tissues
#18
Huatang Zhang, Peng Xiao, Yin Ting Wong, Wei Shen, Mohit Chhabra, Raoul Peltier, Yin Jiang, Yonghe He, Jun He, Yi Tan, Yusheng Xie, Derek Ho, Yun-Wah Lam, Jinpeng Sun, Hongyan Sun
Alkaline phosphatase (ALP) is a family of enzymes involved in the regulation of important biological processes such as cell differentiation and bone mineralization. Monitoring the activity of ALP in serum can help diagnose a variety of diseases including bone and liver diseases. There has been growing interest in developing new chemical tools for monitoring ALP activity in living systems. Such tools will help further delineate the roles of ALP in biological and pathological processes. Previously reported fluorescent probes has a number of disadvantages that limit their application, such as poor selectivity and short-wavelength excitation...
June 23, 2017: Biomaterials
https://www.readbyqxmd.com/read/28660378/mineral-metabolism-abnormalities-in-patients-with-prostate-cancer-a-systematic-case-controlled-study
#19
Francesco Minisola, Cristiana Cipriani, Luciano Colangelo, Mirella Cilli, Alessandro Sciarra, Magnus Von Heland, Luciano Nieddu, Emanuela Anastasi, Roberto Pascone, Valeria Fassino, Daniele Diacinti, Flavia Longo, Salvatore Minisola, Jessica Pepe
PURPOSE: Prostate cancer is the most common tumor in men. To the best of our knowledge a systematic assessment of bone and mineral abnormalities has not been performed in prostatic cancer patients consecutively enrolled. METHODS: This study was therefore carried out to investigate changes of skeletal and mineral metabolism in patients with prostate cancer (n  = 69). A population of patients with cancer of various origin was also investigated as a control group (n  = 53), since a comparison with non-prostate cancer patients has not been previously reported...
June 28, 2017: Endocrine
https://www.readbyqxmd.com/read/28656295/inhibition-of-neuropeptide-y-y1-receptor-induces-osteoblast-differentiation-in-mc3t3%C3%A2-e1-cells
#20
Motoki Yahara, Kanchu Tei, Masato Tamura
Neuropeptide Y (NPY) is a major neural signaling molecule. NPY is produced by peripheral tissues, such as osteoblasts, and binds to the corresponding Y1 receptor that belongs to the G‑protein‑coupled receptor family. Osteoblast‑specific Y1 receptor knockout mice exhibit high bone mass, indicating a role of the NPY‑Y1 receptor axis in the regulation of bone homeostasis. In the bone microenvironment, peripheral nerve fibers and osteoblasts produce NPY. However, the effects of the Y1 receptor on osteoblasts remain unexplored...
September 2017: Molecular Medicine Reports
keyword
keyword
91003
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"