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alginate hydrogel

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https://www.readbyqxmd.com/read/28635256/in-situ-gelation-induced-death-of-cancer-cells-based-on-proteinosomes
#1
Yuting Zhou, Jianmin Song, Lei Wang, Xuting Xue, Xiaoman Liu, Hui Xie, Xin Huang
Hydrogel is quite a nice kind of material, which can be utilized as the platform for cell culture. However, when bulky hydrogel was formed inside cancer cells, results would be changed. In this study, we showed a way of in situ gelation inside the cells which could induce the death of cancer cells. A glutathione (GSH) responsive proteinosomes, with nice biocompatibility, were prepared as carriers of sodium alginate to be endocytosed by cancer cells, where the chelation between sodium alginate and free calcium ions in the culture medium occurred during the diffusion process...
June 21, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28627870/encapsulation-of-autoinducer-sensing-reporter-bacteria-in-reinforced-alginate-based-microbeads
#2
Ping Li, Mareike Müller, Matthew Wook Chang, Martin Frettlöh, Holger Schönherr
Quorum sensing, in which bacteria communities use signaling molecules for inter- and intracellular communication, has been intensively studied in recent decades. In order to fabricate highly sensitive easy-to-handle point of care biosensors that detect quorum sensing molecules, we have developed, as is reported here, reporter bacteria entrapped alginate-methacrylate (alginate-MA) hydrogel beads. The alginate-MA beads, which were obtained by electrostatic extrusion, were reinforced by photo-crosslinking to increase stability and thereby to reduce bacteria leaching...
June 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28627740/biofabrication-of-soft-tissue-templates-for-engineering-the-bone-ligament-interface
#3
Ella Harris, Yurong Liu, Grainne Cuniffe, David Morrissey, Simon Carroll, Kevin Mulhall, Daniel J Kelly
Regenerating damaged tissue interfaces remains a significant clinical challenge, requiring recapitulation of the structure, composition and function of the native enthesis. In the ligament-to-bone interface this region transitions from ligament to fibrocartilage, to calcified cartilage and then to bone. This gradation in tissue types facilitates the transfer of load between soft and hard structures while minimizing stress concentrations at the interface. Previous attempts to engineer the ligament-bone interface have utilized various scaffold materials with an array of various cell types and/or biological cues...
June 19, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28627581/controlled-self-assembly-of-alginate-microgels-by-rapidly-binding-molecule-pairs
#4
Yuebi Hu, Angelo S Mao, Rajiv M Desai, Huanan Wang, David A Weitz, David J Mooney
Controlled self-assembly of cell-encapsulating microscale polymeric hydrogels (microgels) could be advantageous in a variety of tissue engineering and regenerative medicine applications. Here, a method of assembly by chemical modification of alginate polymer with binding pair molecules (BPM) was explored. Alginate was modified with several types of BPM, specifically biotin and streptavidin and click chemistry compounds, and fabricated into 25-30 μm microgels using a microfluidic platform. These microgels were demonstrated to self-assemble under physiological conditions...
June 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28618041/effect-of-various-ratios-of-co-cultured-atdc5-cells-and-chondrocytes-on-the-expression-of-cartilaginous-phenotype-in-microcavitary-alginate-hydrogel
#5
Yongchang Yao, Yuyang Huang, Dongyang Qian, Shujiang Zhang, Yi Chen, Bo Bai
The present study introduced a direct co-culture of mouse ATDC5 cells and primary porcine chondrocytes into a microcavitary hydrogel, which possessed advantages in promoting the growth of chondrocytes and retaining the phenotype. These two types of cells were encapsulated with gelatin microspheres in alginate hydrogels in either of the three ratios (3:1, 1:1, or 1:3 of ATDC5 cells to chondrocytes) and cultured in chondrogenic medium for 28 days. Simultaneously, the single encapsulation of ATDC5 cells or chondrocytes was set as a control...
June 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28603966/silver-alginate-hydrogel-micro-and-nano-containers-for-theranostics-synthesis-encapsulation-remote-release-and-detection
#6
Ekaterina Lengert, Mariia Saveleva, Anatolii Abalymov, Vsevolod Atkin, Pieter C Wuytens, Roman Kamyshinsky, Alexander L Vasiliev, Dmitry A Gorin, Gleb B Sukhorukov, Andre G Skirtach, Bogdan Parakhonskiy
We have designed multifunctional silver alginate hydrogel microcontainers referred to as loaded microcapsules with different sizes by assembling them via a template assisted approach using a natural, highly porous calcium carbonate cores. Sodium alginate was immobilized into the pores of calcium carbonate particles of different sizes followed by cross-linking via addition of silver ions, which had a dual purpose: on one hand, the were used as a crosslinking agent, albeit in the monovalent form, while on the other hand they have led to formation of silver nanoparticles...
June 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28598234/design-and-evaluation-of-novel-topical-formulation-with-olive-oil-as-natural-functional-active
#7
Ana Henriques Mota, Catarina Oliveira Silva, Marisa Nicolai, André Baby, Lídia Palma, Patrícia Rijo, Lia Ascensão, Catarina Pinto Reis
Currently, the innovative skin research is focused on the development of novel topical formulations loaded with natural functional actives. The health benefits of olive oil are unsurpassed and many others are revealed as research studies allow the understanding of its unlimited properties. Olive oil has a protective toning effect on skin, but it is not transported effectively into its layers. Aiming the development of a cosmetic formulation for skin photoprotection and hydration, we have prepared and characterized macro-sized particles, made of a hydrogel polymer, loaded with olive oil...
June 9, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28598154/3d-printable-bioactivated-nanocellulose-alginate-hydrogels
#8
Jenni Leppiniemi, Panu Lahtinen, Antti Paajanen, Riitta Mahlberg, Sini Metsä-Kortelainen, Tatu Pinomaa, Heikki Pajari, Inger Marianne Vikholm-Lundin, Pekka Pursula, Vesa P Hytönen
We describe a nanocellulose-alginate hydrogel suitable for 3D printing. The composition of the hydrogel was optimized based on material characterization methods and 3D printing experiments, and its behavior during the printing process was studied using computational fluid dynamics simulations. The hydrogel was biofunctionalized by covalent coupling of an enhanced avidin protein to the cellulose nanofibrils. Ionic cross-linking of the hydrogel using calcium ions improved the performance of the material. The resulting hydrogel is suitable for 3D printing, its mechanical properties indicate good tissue compatibility, and the hydrogel absorbs water in moist conditions, suggesting potential in applications such as wound dressings...
June 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28590722/injectable-fullerenol-alginate-hydrogel-for-suppression-of-oxidative-stress-damage-in-brown-adipose-derived-stem-cells-and-cardiac-repair
#9
Tong Hao, Junjie Li, Fanglian Yao, Dianyu Dong, Yan Wang, Boguang Yang, Changyong Wang
Stem cell implantation strategy has exhibit potential to treat the myocardial infarction (MI), however, the low retention and survival limit their applications due to the reactive oxygen species (ROS) microenvironment after MI. In this study, the fullerenol nanoparticles are introduced into alginate hydrogel to create an injectable cell delivery vehicle with antioxidant activity. Results suggest that the prepared hydrogels exhibit excellent injectable and mechanical strength. In addition, the fullerenol/alginate hydrogel can effectively scavenge the superoxide anion and hydroxyl radicals...
June 7, 2017: ACS Nano
https://www.readbyqxmd.com/read/28589747/a-novel-bio-electro-active-alginate-aniline-tetramer-agarose-scaffold-for-tissue-engineering-synthesis-characterization-drug-release-and-cell-culture-study
#10
Zhale Atoufi, Payam Zarrintaj, Ghodratollah Hashemi Motlagh, Anahita Amiri, Zohreh Bagher, Seyed Kamran Kamrava
In this study, synthesis of a novel biocompatible stimuli-responsive conducting hydrogel based on agarose/alginate-aniline tetramer with the capability of a tailored electrically controlled drug-release for neuroregeneration is investigated. First, aniline tetramer is synthesized and grafted onto sodium alginate. Then, this material is added to agarose as an electrical conductivity modifier to obtain Agarose/alginate-aniline tetramer hydrogel. The synthesized materials are characterized by H NMR and FTIR. The hydrogels are prepared with varying content of aniline tetramer and their swelling-deswelling and shape memory behavior is evaluated...
June 14, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28583017/three-dimensional-prostate-tumor-model-based-on-a-hyaluronic-acid-alginate-hydrogel-for-evaluation-of-anti-cancer-drug-efficacy
#11
Yadong Tang, Boxin Huang, Yuqin Dong, Wenlong Wang, Xi Zheng, Wei Zhou, Kun Zhang, Zhiyun Du
In vitro cell-based assays are widely applied to evaluate anti-cancer drug efficacy. However, the conventional approaches are mostly based on two-dimensional (2D) culture systems, making it difficult to recapitulate the in vivo tumor scenario because of spatial limitations. Here, we develop an in vitro three-dimensional (3D) prostate tumor model based on a hyaluronic acid (HA)-alginate hybrid hydrogel to bridge the gap between in vitro and in vivo anticancer drug evaluations. In situ encapsulation of PCa cells was achieved by mixing HA and alginate aqueous solutions in the presence of cells and then crosslinking with calcium ions...
June 8, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28582709/electrodepositable-alginate-membranes-for-enzymatic-sensors-an-amperometric-glucose-biosensor-for-whole-blood-analysis
#12
A Márquez, C Jiménez-Jorquera, C Domínguez, X Muñoz-Berbel
Simple and disposable point of care systems are usually the best solution for chronic patients to get a rapid diagnosis in home care context. However, their main drawback relies on the poor reliability derived from the low stability of the bio-recognition elements and low quality of the transducers. In the current work, we study the use of electrodeposited calcium alginate hydrogels as a biocompatible matrix in the development of enzymatic amperometric biosensors for whole blood analysis, to enhance the enzymes stability and to protect the transducer from biofouling...
May 30, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28576052/facile-in-situ-formation-of-hybrid-gels-for-direct-forming-tissue-engineering
#13
Jun-Sung Oh, Jeong-Soon Park, Cheol-Min Han, Eun-Jung Lee
Development of bioactive hydrogel as extracellular matrix (ECM) is a very important field for cell-based therapy. In this study, we provided a facile method based on sol-gel process for fabricating bioactive composite hydrogels. The composite hydrogels were composed of sol-gel derived silica and biopolymer. Different amounts of silica solution (20-80wt%) were mixed with 2% polymer sol (alginate) followed by aging and gelation to form a network so that the alginate-silica hybrid mixture could form a gel without any additional crosslinking process...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28575938/tailor-made-alginate-hydrogel-for-local-infection-prophylaxis-in-orthopedic-applications
#14
K Sangeetha, E K Girija
Preventing implants associated infections is crucial in orthopedics. Local delivery of antibiotics through implants is a promising strategy to solve this issue. In this study, alginate is tailored to control its swelling, entrapment and release of ciprofloxacin (antibiotic) through the formation of interpenetrating polymer network and composite matrices using gelatin and hydroxyapatite. Developed matrices were characterized by Fourier transform infrared spectroscopy, x-ray diffraction and scanning electron microscopy...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28557440/construction-of-injectable-double-network-hydrogels-for-cell-delivery
#15
Yan Yan, Mengnan Li, Di Yang, Qian Wang, Fuxin Liang, Xiaozhong Qu, Dong Qiu, Zhenzhong Yang
Herein we present a unique method of using dynamic crosslinks, i.e. dynamic covalent bonding and ionic interaction, for the construction of injectable double-network (DN) hydrogels, with the objective of cell delivery for cartilage repair. Glycol chitosan and di-benzaldhyde capped poly(ethylene oxide) formed the first network while calcium alginate formed the second one, and in the resultant DN hydrogel, either of the networks could be selectively removed. The moduli of the DN hydrogel were significantly improved compared to that of the parent single-network hydrogels and were tunable by changing the chemical components...
May 30, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28544472/engineering-muscle-cell-alignment-through-3d-bioprinting
#16
Pamela Mozetic, Sara Maria Giannitelli, Manuele Gori, Marcella Trombetta, Alberto Rainer
Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell-seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic(®) ) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment...
May 22, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28541940/efficient-removal-of-cd-2-ion-from-water-by-calcium-alginate-hydrogel-filtration-membrane
#17
Ye Li, Kongyin Zhao, Wei Yang, Guanyi Chen, Xinxin Zhang, Yujie Zhao, Liang Liu, Min Chen
Calcium alginate (CaAlg) hydrogel filtration membrane was prepared using urea as pore-forming agent. The effects of preparation and operating conditions on the removal rate of Cd(2+) were researched. The removal mechanism of Cd(2+) and the anti-fouling property of CaAlg membrane were investigated. The removal rate of the CaAlg filtration membrane reached over 99.5% within 120 min when 20 mg/L Cd(2+) was used, and the flux was 15.5 L/m(2)h at 0.1 MPa when the thickness of the membrane was 0.28 ± 0.08 mm. However, the removal rate of Cd(2+) was below 10...
May 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28540461/quantitative-analysis-of-curcumin-loaded-alginate-nanocarriers-in-hydrogels-using-raman-and-attenuated-total-reflection-infrared-spectroscopy
#18
Lynda Miloudi, Franck Bonnier, Dominique Bertrand, Hugh J Byrne, Xavier Perse, Igor Chourpa, Emilie Munnier
Core-shell nanocarriers are increasingly being adapted in cosmetic and dermatological fields, aiming to provide an increased penetration of the active pharmaceutical or cosmetic ingredients (API and ACI) through the skin. In the final form, the nanocarriers (NC) are usually prepared in hydrogels, conferring desired viscous properties for topical application. Combined with the high chemical complexity of the encapsulating system itself, involving numerous ingredients to form a stable core and quantifying the NC and/or the encapsulated active without labor-intensive and destructive methods remains challenging...
May 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28539292/-gelatin-alginate-hydrogel-scaffolds-prepared-by-3d-bioprinting-promotes-cell-adhesion-and-proliferation-of-human-dental-pulp-cells-in-vitro
#19
Hai-Yue Yu, Dan-Dan Ma, Bu-Ling Wu
OBJECTIVE: To evaluate the cytotoxicity of gelatin/alginate hydrogel scaffolds prepared by 3D bioprinting in human dental pulp cells (HDPCs) and compare the cell adhesion and proliferation of the cells seeded in the biomaterial using two different methods. METHODS: HDPCs isolated by tissue block culture and enzyme digestion were cultured and passaged. Gelatin/alginate hydrogel scaffolds were printed using a bioplotter, and the cytotoxicity of the aqueous extracts of the scaffold material was tested in the third passage of HDPCs using cell counting kit-8...
May 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28537482/programmed-platelet-derived-growth-factor-%C3%AE-%C3%AE-and-bone-morphogenetic-protein-2-delivery-from-a-hybrid-calcium-phosphate-alginate-scaffold
#20
Emily A Bayer, Jahnelle Jordan, Riccardo Gottardi, Morgan Fedorchak, Prashant N Kumta, Steven Little
Bone tissue engineering requires the upregulation of several regenerative stages, including a critical early phase of angiogenesis. Previous studies have suggested that a sequential delivery of PDGF to BMP-2 could promote angiogenic tubule formation when delivered to in vitro co-cultures of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (hMSCs). However, it was previously unclear that this PDGF to BMP-2 delivery schedule will result in cell migration into the scaffolding system and affect the later expression of bone markers...
May 24, 2017: Tissue Engineering. Part A
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