keyword
MENU ▼
Read by QxMD icon Read
search

Laura

keyword
https://www.readbyqxmd.com/read/29223107/recreation-economics-to-inform-migratory-species-conservation-case-study-of-the-northern-pintail
#1
Brady J Mattsson, James A Dubovsky, Wayne E Thogmartin, Kenneth J Bagstad, Joshua H Goldstein, John B Loomis, James E Diffendorfer, Darius J Semmens, Ruscena Wiederholt, Laura López-Hoffman
Quantification of the economic value provided by migratory species can aid in targeting management efforts and funding to locations yielding the greatest benefits to society and species conservation. Here we illustrate a key step in this process by estimating hunting and birding values of the northern pintail (Anas acuta) within primary breeding and wintering habitats used during the species' annual migratory cycle in North America. We used published information on user expenditures and net economic values (consumer surplus) for recreational viewing and hunting to determine the economic value of pintail-based recreation in three primary breeding areas and two primary wintering areas...
December 6, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/29223000/bilateral-preictal-signature-of-phase-amplitude-coupling-in-canine-epilepsy
#2
Laura Gagliano, Elie Bou Assi, Dang K Nguyen, Sandy Rihana, Mohamad Sawan
Seizure forecasting would improve the quality of life of patients with refractory epilepsy. Although early findings were optimistic, no single feature has been found capable of individually characterizing brain dynamics during transition to seizure. Cross-frequency phase amplitude coupling has been recently proposed as a precursor of seizure activity. This work evaluates the existence of a statistically significant difference in mean phase amplitude coupling distribution between the preictal and interictal states of seizures in dogs with bilaterally implanted intracranial electrodes...
November 26, 2017: Epilepsy Research
https://www.readbyqxmd.com/read/29222993/supplementation-of-gamma-aminobutyric-acid-gaba-affects-temporal-but-not-spatial-visual-attention
#3
Anna Leonte, Lorenza S Colzato, Laura Steenbergen, Bernhard Hommel, Elkan G Akyürek
In a randomized, double-blind, and placebo-controlled experiment, the acute effects of gamma-aminobutyric acid (GABA) supplementation on temporal and spatial attention in young healthy adults were investigated. A hybrid two-target rapid serial visual presentation task was used to measure temporal attention and integration. Additionally, a visual search task was used to measure the speed and accuracy of spatial attention. While temporal attention depends primarily on the distribution of limited attentional resources across time, spatial attention represents the engagement and disengagement by relevant and irrelevant stimuli across the visual field...
December 6, 2017: Brain and Cognition
https://www.readbyqxmd.com/read/29222790/site-specific-direct-labeling-of-neurotrophins-and-their-receptors-from-biochemistry-to-advanced-imaging-applications
#4
Francesco Gobbo, Fulvio Bonsignore, Rosy Amodeo, Antonino Cattaneo, Laura Marchetti
We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222789/growth-and-neurotrophic-factors-in-embryonic-stem-cells
#5
Micaela Pannella, Luciana Giardino, Laura Calzà, Mercedes Fernández
In this chapter we illustrate protocols to investigate growth and neurotrophic factors in human and rodent (rat and mouse)-derived embryonic stem cells. The conventional two-dimensional cell monolayer system to grow embryonic stem cells is presented, focusing on the coating strategies also using extracellular matrix components. Then, different approaches for three-dimensional stem cell culture are presented, using hydrogels and scaffolds. Quantitative polymerase chain reaction, immunocytochemistry, immunoenzymatic ELISA assay, and multiparametric assays to quantify growth and neurotrophic factor production are presented...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222781/cell-enumeration-assays-application-of-the-mtt-and-sulforhodamine-b-assays-to-lipopolysaccharide-stimulated-neonatal-rodent-microglia
#6
Laura Facci, Stephen D Skaper
Glial cell activation, in particular microglia, plays an important role in the pathogenesis of various neurodegenerative disorders as well as in chronic and neuropathic pain. This chapter compares two established cell enumeration assays, namely, the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the protein-binding sulforhodamine B assay for microglia as a function of culture condition and activation state. The pros and cons of each are then described.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222780/oligodendrocyte-progenitor-cell-cultures-a-model-to-screen-neurotrophic-compounds-for-myelin-repair
#7
Massimo Barbierato, Stephen D Skaper, Laura Facci
The protocol presented in this chapter covers the application of rat cortical oligodendrocyte progenitor cells cultured under conditions of differentiation for the evaluation of agents with potential trophic activity, that is, which are capable of promoting maturation under such conditions. As an example we have chosen a co-ultramicronized N-palmitoylethanolamine/luteolin composite, a formulation described in the literature as possessing anti-inflammatory, neuroprotective, and neuroregenerative actions.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222778/astrocyte-microglia-cocultures-as-a-model-to-study-neuroinflammation
#8
Laura Facci, Massimo Barbierato, Stephen D Skaper
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders as well as in chronic and neuropathic pain. This chapter describes a model which allows one to assess the individual and combined contributions of astrocytes and microglia in response to a pro-inflammatory stimulus, with emphasis on ionotropic purinergic receptors.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222776/culture-of-rat-mesencephalic-dopaminergic-neurons-and-application-to-neurotoxic-and-neuroprotective-agents
#9
Stephen D Skaper, Massimo Barbierato, Vanni Ferrari, Morena Zusso, Laura Facci
Dopaminergic neuronal cell degeneration is the principal characteristic feature of the neuropathology of Parkinson disease. Cultures of mesencephalic neurons are widely used as a source of dopaminergic neurons for the study of mechanisms implicated in dopaminergic cell death and for the evaluation of potential dopaminergic neuroprotective agents, including neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat mesencephalic cell cultures and their application to evaluating the effect of the dopaminergic neurotoxin 1-methyl-4-phenylpyridinium and the neuroprotective action of brain-derived neurotrophic factor...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222773/central-nervous-system-neuron-glia-co-culture-models-and-application-to-neuroprotective-agents
#10
Stephen D Skaper, Laura Facci
Glial cell activation plays an important role in the pathogenesis of various neurodegenerative disorders. This article presents a protocol for the preparation of cultures consisting of rat embryonic cortical neurons grown in the presence of cortical microglia, in which the glia are present in physical contact with the neurons or separated by a semipermeable membrane barrier. An example of how such systems can be used to evaluate potential neuroprotective agents will also be described.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222772/culture-of-neonatal-rodent-microglia-astrocytes-and-oligodendrocytes-from-the-cortex-spinal-cord-and-cerebellum
#11
Stephen D Skaper, Laura Facci
The protocol described in this chapter covers the preparation and culture of enriched populations of microglia, astrocytes, and oligodendrocytes from the cortex and spinal cord of neonatal rat and mouse. The procedure is based on enzymatic digestion of the tissue, followed by the culture of a mixed glial cell population which is then utilized as the starting point for the isolation, via differential attachment, of the different cell types.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222771/culture-of-rodent-cortical-hippocampal-and-striatal-neurons
#12
Laura Facci, Stephen D Skaper
Neurons cultured from rodent central nervous system tissues represent important tools in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical, hippocampal, and striatal neuron cell cultures, using either embryonic or postnatal tissue with enzymatic digestion.
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29222652/development-of-a-kelp-powder-thallus-laminariae-standard-reference-material
#13
Lee L Yu, Joseph F Browning, Carolyn Q Burdette, George C Caceres, Kaitlyn D Chieh, W Clay Davis, Brittany L Kassim, Stephen E Long, Karen E Murphy, Rabia Oflaz, Rick L Paul, Katherine E Sharpless, Laura J Wood, James H Yen, Rolf Zeisler
A Standard Reference Material (SRM) of seaweed, SRM 3232 Kelp Powder (Thallus laminariae) has been developed to support food and dietary supplement measurements in compliance with the Food Safety Modernization Act (FSMA) and the Dietary Supplement Health and Education Act of 1994 (DSHEA). The material was characterized for nutritional minerals, arsenic species, isomers of vitamin K1, proximates, and toxic elements. Kelp is a rich source of vitamins and minerals, and it is an excellent source of dietary iodine...
December 9, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29222541/estimating-cost-effectiveness-of-a-multimodal-ovarian-cancer-screening-program-in-the-united-states-secondary-analysis-of-the-uk-collaborative-trial-of-ovarian-cancer-screening-ukctocs
#14
Haley A Moss, Andrew Berchuck, Megan L Neely, Evan R Myers, Laura J Havrilesky
Importance: The United Kingdom Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) is the largest randomized clinical trial to evaluate screening's impact on ovarian cancer mortality, assigning women to multimodal screening (MMS) with serum cancer antigen 125 (CA-125) interpreted using a risk algorithm. If the MMS screening method is eventually shown to reduce mortality and be cost-effective, then it may be accepted by the medical community as a feasible screening tool. Objective: To estimate the cost-effectiveness of an MMS screening program in the United States...
December 7, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/29222517/input-dependent-regulation-of-excitability-controls-dendritic-maturation-in-somatosensory-thalamocortical-neurons
#15
Laura Frangeul, Vassilis Kehayas, Jose V Sanchez-Mut, Sabine Fièvre, K Krishna-K, Gabrielle Pouchelon, Ludovic Telley, Camilla Bellone, Anthony Holtmaat, Johannes Gräff, Jeffrey D Macklis, Denis Jabaudon
Input from the sensory organs is required to pattern neurons into topographical maps during development. Dendritic complexity critically determines this patterning process; yet, how signals from the periphery act to control dendritic maturation is unclear. Here, using genetic and surgical manipulations of sensory input in mouse somatosensory thalamocortical neurons, we show that membrane excitability is a critical component of dendritic development. Using a combination of genetic approaches, we find that ablation of N-methyl-D-aspartate (NMDA) receptors during postnatal development leads to epigenetic repression of Kv1...
December 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/29222075/examining-tensions-that-affect-the-evaluation-of-technology-in-health-care-considerations-for-system-decision-makers-from-the-perspective-of-industry-and-evaluators
#16
Laura Desveaux, James Shaw, Ross Wallace, Onil Bhattacharyya, R Sacha Bhatia, Trevor Jamieson
Virtual technologies have the potential to mitigate a range of challenges for health care systems. Despite the widespread use of mobile devices in everyday life, they currently have a limited role in health service delivery and clinical care. Efforts to integrate the fast-paced consumer technology market with health care delivery exposes tensions among patients, providers, vendors, evaluators, and system decision makers. This paper explores the key tensions between the high bar for evidence prior to market approval that guides health care regulatory decisions and the "fail fast" reality of the technology industry...
December 8, 2017: JMIR Medical Informatics
https://www.readbyqxmd.com/read/29222006/wrist-accelerometry-for-physical-activity-measurement-in-individuals-with-spinal-cord-injury-a-need-for-individually-calibrated-cut-points
#17
Laura A McCracken, Jasmin K Ma, Christine Voss, Franco H N Chan, Kathleen A Martin Ginis, Christopher R West
OBJECTIVE: To create and compare individual and group-based cut-points for wrist-accelerometry that corresponds to moderate-to-vigorous physical activity (MVPA) in people with spinal cord injury (SCI). DESIGN: Participants completed a graded treadmill-wheeling test while being assessed for oxygen consumption, wrist-acceleration vector magnitude, and spoke acceleration. Oxygen consumption was converted to SCI metabolic equivalents (SCI METS) and linear regression was applied to determine an individualized vector magnitude cut-point (counts per minute, VM-CPM) corresponding with MVPA (≥3 SCI METS)...
December 5, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/29221946/-facing-a-dilemma-in-elderly-complex-and-vulnerable-patients-to-stop-or-not-to-stop-prevention
#18
Laura Llobet Vila, Josep Maria Manresa Domínguez, José Manuel Carmona Segado, Alícia Franzi Sisó, Rosa Vallès Balasch, Isabel Martínez Díaz
OBJECTIVES: 1-To know the preventive prescription (statin, antiplatelet drugs, bisphosphonates, calcium and vitamin D and Vit B12) of elderly patients previously identified as a CCD (with a complex chronic disease) and ACD (with advanced chronic disease and lifetime expectancy of less than 12 months) who died during 2015. 2-To assess the correct indication of Proton Pump Inhibitors (PPI) to determine potentially avoidable medication. DESIGN: Retrospective observational study...
December 5, 2017: Atencion Primaria
https://www.readbyqxmd.com/read/29221798/association-of-heterozygous-ccr5%C3%AE-32-deletion-with-survival-in-hiv-infection-a-cohort-study
#19
Ezequiel Ruiz-Mateos, Laura Tarancon-Diez, A I Alvarez-Rios, Beatriz Dominguez-Molina, Miguel Genebat, Ildefonso Pulido, Maria Antonia Abad, Maria Angeles Muñoz-Fernandez, Manuel Leal
The role of a 32 base pair deletion in the CCR5 gene (CCR5Δ32) in HIV-disease progression and response to combined antiretroviral therapy (cART) is well established. However, the impact of CCR5Δ32 in the long-term survival pre-cART and after cART introduction in a large cohort of HIV-infected patients is unknown. We analyzed the association of CCR5Δ32 deletion in the long-term survival of HIV-infected patients recruited between June 1981 and October 2016 (n = 1006). Clinical and epidemiological variables were recorded and CCR5Δ32 deletion was assessed by PCR and electrophoretic analysis...
December 5, 2017: Antiviral Research
https://www.readbyqxmd.com/read/29221774/sleep-dependent-motor-sequence-memory-consolidation-in-individuals-with-periodic-limb-movements
#20
Valya Sergeeva, Jeremy Viczko, Laura B Ray, Adrian M Owen, Stuart M Fogel
Periodic limb movements (PLMs) during sleep increase with age and are associated with striatal neurodegeneration and dopamine deficiency. Limb movements are often associated with disruptions to non-rapid eye movement (NREM) sleep. Motor skill memory consolidation recruits the striatum, and learning-dependent striatal activation is associated with NREM sleep. Therefore, we investigated whether de novo individuals who significantly experience elevated levels of PLMs but have not been formally diagnosed with periodic limb movement disorder had learning and sleep-related memory deficits and whether these deficits were related to sleep quality and symptom severity...
December 2017: Sleep Medicine
keyword
keyword
9093
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"