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in vivo whole cell recording

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https://www.readbyqxmd.com/read/28424616/whole-blood-cytokine-response-to-local-traffic-related-particulate-matter-in-peruvian-children-with-and-without-asthma
#1
Jesse P Negherbon, Karina Romero, D'Ann L Williams, Rafael E Guerrero-Preston, Thomas Hartung, Alan L Scott, Patrick N Breysse, William Checkley, Nadia N Hansel
This study sought to investigate if acute phase immune responses of whole blood from Peruvian children with controlled and uncontrolled asthma differed from children without asthma, following exposure to traffic-related particulate matter (TRPM). TRPM, including particulate matter from diesel combustion, has been shown to stimulate acute airway inflammation in individuals with and without asthma. For this study, a whole blood assay (WBA) was used to test peripheral whole blood samples from 27 children with asthma, and 12 without asthma...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28423316/diversity-in-excitation-inhibition-mismatch-underlies-local-functional-heterogeneity-in-the-rat-auditory-cortex
#2
Can Tao, Guangwei Zhang, Chang Zhou, Lijuan Wang, Sumei Yan, Huizhong Whit Tao, Li I Zhang, Yi Zhou, Ying Xiong
Cortical neurons are heterogeneous in their functional properties. This heterogeneity is fundamental for the processing of different features of sensory information. However, functional diversity within a local group of neurons is poorly understood. Here, we demonstrate that neighboring cortical neurons in layer 5 but not those of layer 4 of the rat anterior auditory field (AAF) exhibited a surprisingly high level of diversity in tonal receptive fields. In vivo whole-cell voltage-clamp recordings revealed that the diversity of frequency representation was due to a spectral mismatch between synaptic excitation and inhibition to varying degrees...
April 18, 2017: Cell Reports
https://www.readbyqxmd.com/read/28415676/nitrobenzylthioinosine-mimics-adenosine-to-attenuate-the-epileptiform-discharge-of-hippocampal-neurons-from-epileptic-rats
#3
Hao Huang, Jing Wang, Jun Zhang, Zhong Luo, Dongxu Li, Xiaowei Qiu, Yan Peng, Zhongxiang Xu, Ping Xu, Zucai Xu
Nitrobenzylthioinosine (NBTI), a specific inhibitor of type 1 equilibrative nucleoside transporter, could regulate the extracellular adenosine concentration and have protective roles in seizures. However, the protection mechanism of NBTI in seizures remains poorly understood. Here, the expression pattern and subcellular distribution of adenosine A1 receptor were detected by Western blot analysis and double-labeling immunofluorescence staining in Lithium Chloride-Pilocarpine induced epileptic rat model. At 24 h after pilocarpine induced rat seizures, hippocampal slices were prepared and the evoked excitatory postsynaptic currents (eEPSCs) amplitude of pyramidal neurons in hippocampus CA1 region was recorded using whole-cell patch clamp...
March 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28394013/intermittent-theta-burst-stimulation-rescues-dopamine-dependent-corticostriatal-synaptic-plasticity-and-motor-behavior-in-experimental-parkinsonism-possible-role-of-glial-activity
#4
Fabrizio Cacace, Desirèe Mineo, Maria Teresa Viscomi, Emanuele Claudio Latagliata, Maria Mancini, Valeria Sasso, Anna Vannelli, Tiziana Pascucci, Valentina Pendolino, Elena Marcello, Silvia Pelucchi, Stefano Puglisi-Allegra, Marco Molinari, Barbara Picconi, Paolo Calabresi, Veronica Ghiglieri
BACKGROUND: Recent studies support the therapeutic utility of repetitive transcranial magnetic stimulation in Parkinson's disease (PD), whose progression is correlated with loss of corticostriatal long-term potentiation and long-term depression. Glial cell activation is also a feature of PD that is gaining increasing attention in the field because astrocytes play a role in chronic neuroinflammatory responses but are also able to manage dopamine (DA) levels. METHODS: Intermittent theta-burst stimulation protocol was applied to study the effect of therapeutic neuromodulation on striatal DA levels measured by means of in vivo microdialysis in 6-hydroxydopamine-hemilesioned rats...
April 10, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/28373550/resistance-to-action-potential-depression-of-a-rat-axon-terminal-in-vivo
#5
Martijn C Sierksma, J Gerard G Borst
The shape of the presynaptic action potential (AP) has a strong impact on neurotransmitter release. Because of the small size of most terminals in the central nervous system, little is known about the regulation of their AP shape during natural firing patterns in vivo. The calyx of Held is a giant axosomatic terminal in the auditory brainstem, whose biophysical properties have been well studied in slices. Here, we made whole-cell recordings from calyceal terminals in newborn rat pups. The calyx showed a characteristic burst firing pattern, which has previously been shown to originate from the cochlea...
April 18, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28373496/in-vivo-patch-clamp-recording-in-awake-head-fixed-rodents
#6
Doyun Lee, Albert K Lee
Whole-cell recording has been used to measure and manipulate a neuron's spiking and subthreshold membrane potential, allowing assessment of the cell's inputs and outputs as well as its intrinsic membrane properties. This technique has also been combined with pharmacology and optogenetics as well as morphological reconstruction to address critical questions concerning neuronal integration, plasticity, and connectivity. This protocol describes a technique for obtaining whole-cell recordings in awake head-fixed animals, allowing such questions to be investigated within the context of an intact network and natural behavioral states...
April 3, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28349479/mesenchymal-stromal-cells-as-active-regulators-of-lymphocyte-recruitment-to-blood-vascular-endothelial-cells
#7
Helen M Mcgettrick, Lewis S C Ward, George Edward Rainger, Gerard B Nash
Methods are described for analyzing adhesion and migration of isolated lymphocytes on endothelial cell monolayers which have been cocultured with different mesenchymal stromal cells, with or without additional cytokine treatment. The different cells types are grown on opposite sides of 3.0 or 0.4 μm pore filters, depending on whether migration through the whole construct is to be analyzed, or adhesion to the endothelial cells alone. Migration away from the sub-endothelial space and through the stromal layer can also be assessed by culturing mesenchymal stromal cells within a 3-D collagen gel overlaid with endothelial cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28334383/a-critical-role-of-inhibition-in-temporal-processing-maturation-in-the-primary-auditory-cortex
#8
Dongqin Cai, Rongrong Han, Miaomiao Liu, Fenghua Xie, Ling You, Yi Zheng, Limin Zhao, Jun Yao, Yiwei Wang, Yin Yue, Christoph E Schreiner, Kexin Yuan
Faithful representation of sound envelopes in primary auditory cortex (A1) is vital for temporal processing and perception of natural sounds. However, the emergence of cortical temporal processing mechanisms during development remains poorly understood. Although cortical inhibition has been proposed to play an important role in this process, direct in-vivo evidence has been lacking. Using loose-patch recordings in rat A1 immediately after hearing onset, we found that stimulus-following ability in fast-spiking neurons was significantly better than in regular-spiking (RS) neurons...
March 1, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28325790/dopamine-terminals-from-the-ventral-tegmental-area-gate-intrinsic-inhibition-in-the-prefrontal-cortex
#9
William C Buchta, Stephen V Mahler, Benjamin Harlan, Gary S Aston-Jones, Arthur C Riegel
Spike frequency adaptation (SFA or accommodation) and calcium-activated potassium channels that underlie after-hyperpolarization potentials (AHP) regulate repetitive firing of neurons. Precisely how neuromodulators such as dopamine from the ventral tegmental area (VTA) regulate SFA and AHP (together referred to as intrinsic inhibition) in the prefrontal cortex (PFC) remains unclear. Using whole cell electrophysiology, we measured intrinsic inhibition in prelimbic (PL) layer 5 pyramidal cells of male adult rats...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28314951/relaxin-3-rxfp3-signalling-in-mouse-hypothalamus-no-effect-of-rxfp3-activation-on-corticosterone-despite-reduced-presynaptic-excitatory-input-onto-paraventricular-crh-neurons-in-vitro
#10
C Zhang, D V Baimoukhametova, C M Smith, J S Bains, Andrew L Gundlach
Relaxin-3/RXFP3 signalling is proposed to be involved in the neuromodulatory control of arousal- and stress-related neural circuits. Furthermore, previous studies in rats have led to the proposal that relaxin-3/RXFP3 signalling is associated with activation of the hypothalamic-pituitary-adrenal axis, but direct evidence for RXFP3-related actions on the activity of hypothalamic corticotropin-releasing hormone (CRH) neurons is lacking. In this study, we investigated characteristics of the relaxin-3/RXFP3 system in mouse hypothalamus...
March 17, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28299825/fingolimod-gilenya-%C3%A2-in-multiple-sclerosis-bradycardia-atrioventricular-blocks-and-mild-effect-on-the-qtc-interval-something-to-do-with-the-l-type-calcium-channel
#11
Sylvie Pilote, Chantale Simard, Benoit Drolet
Cardiac arrhythmias and ECG abnormalities including bradycardia, prolongation of the QT interval and atrioventricular (AV) conduction blocks have been extensively observed with fingolimod, the first marketed oral drug for treating the relapsing-remitting form of multiple sclerosis. The present study was aiming to further elucidate the effects of fingolimod on cardiac electrophysiology at three different levels; 1) in vitro, 2) ex vivo and 3) in vivo. 1) Patch-clamp experiments in whole cell configuration were performed on Cav 1...
March 16, 2017: Fundamental & Clinical Pharmacology
https://www.readbyqxmd.com/read/28294161/in-vivo-imaging-of-the-muc5b-gel-forming-mucin
#12
Céline Portal, Valérie Gouyer, Mylène Magnien, Ségolène Plet, Frédéric Gottrand, Jean-Luc Desseyn
Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b production in mouse tissues by immunofluorescence microscopy and fluorescent activity using stereromicroscopy and probe-based confocal laser endomicroscopy. Muc5b production was followed longitudinally by recording the fluorescent activity in vagina and in embryonic lung explants under stimulation by interleukin 13...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28263785/nociceptive-neuronal-fc-gamma-receptor-i-is-involved-in-igg-immune-complex-induced-pain-in-the-rat
#13
Haowu Jiang, Xinhua Shen, Zhiyong Chen, Fan Liu, Tao Wang, Yikuan Xie, Chao Ma
Antigen-specific immune diseases such as rheumatoid arthritis are often accompanied by pain and hyperalgesia. Our previous studies have demonstrated that Fc-gamma-receptor type I (FcγRI) is expressed in a subpopulation of rat dorsal root ganglion (DRG) neurons and can be directly activated by IgG immune complex (IgG-IC). In this study we investigated whether neuronal FcγRI contributes to antigen-specific pain in the naïve and rheumatoid arthritis model rats. In vitro calcium imaging and whole-cell patch clamp recordings in dissociated DRG neurons revealed that only the small-, but not medium- or large-sized DRG neurons responded to IgG-IC...
March 2, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28244309/functional-roles-of-the-edited-isoform-of-glua2-in-glua2-containing-ampa-receptor-channels
#14
Yan Han, Chi-Yen Lin, Li Niu
GluA2R is the edited isoform at the Q/R site of the AMPA receptor GluA2 subunit. In vivo, GluA2R assembles with other AMPA receptor subunits into a high-conductance, tetrameric complex. Here we study the functional role of GluA2R in the context of GluA1/2R and GluA2Q/2R heteromeric receptors, using whole-cell recording and a laser-pulse photolysis technique. We find the incorporation of GluA2R with GluA1 or GluA2Q slows the channel-opening and channel-closing rates; for GluA2Q/2R, it further lowers EC50, compared to the value of the GluA2Q homomeric channels...
March 9, 2017: Biochemistry
https://www.readbyqxmd.com/read/28214516/saturation-of-long-term-potentiation-in-the-dorsal-cochlear-nucleus-and-its-pharmacological-reversal-in-an-experimental-model-of-tinnitus
#15
Thomas Tagoe, Daniel Deeping, Martine Hamann
Animal models have demonstrated that tinnitus is a pathology of dysfunctional excitability in the central auditory system, in particular in the dorsal cochlear nucleus (DCN) of the brainstem. We used a murine model and studied whether acoustic over-exposure leading to hearing loss and tinnitus, affects long-term potentiation (LTP) at DCN multisensory synapses. Whole cell and field potential recordings were used to study the effects on release probability and synaptic plasticity, respectively in brainstem slices...
February 16, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/28182698/prevention-of-remifentanil-induced-postoperative-hyperalgesia-by-dexmedetomidine-via-regulating-the-trafficking-and-function-of-spinal-nmda-receptors-as-well-as-pkc-and-camkii-level-in-vivo-and-in-vitro
#16
Yuan Yuan, Zhe Sun, Yi Chen, Yuxin Zheng, Ke-Liang Xie, Ying He, Zhifen Wang, Guo-Lin Wang, Yong-Hao Yu
Remifentanil-induced secondary hyperalgesia has been demonstrated in both animal experiments and clinical trials. Enhancement of N-methyl-D-aspartate (NMDA) receptor trafficking as well as protein kinase C (PKC) and calmodulin-dependent protein kinase II (CaMKII) have been reported to be involved in the induction and maintenance of central sensitization. In the current study, it was demonstrated that dexmedetomidine could prevent remifentanil-induced hyperalgesia (RIH) via regulating spinal NMDAR-PKC-Ca2+/ CaMKII pathway in vivo and in vitro...
2017: PloS One
https://www.readbyqxmd.com/read/28139019/from-single-cells-and-single-columns-to-cortical-networks-dendritic-excitability-coincidence-detection-and-synaptic-transmission-in-brain-slices-and-brains
#17
Bert Sakmann
Although patch pipettes were initially designed to record extracellularly the elementary current events from muscle and neuron membranes, the whole-cell and loose cell-attached recording configurations proved to be useful tools for examination of signalling within and between nerve cells. In this Paton Prize Lecture, I will initially summarize work on electrical signalling within single neurons, describing communication between the dendritic compartments, soma and nerve terminals via forward- and backward-propagating action potentials...
January 31, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28126496/glycogen-synthase-kinase-3-beta-alters-anxiety-depression-and-addiction-related-behaviors-and-neuronal-activity-in-the-nucleus-accumbens-shell
#18
Elizabeth J Crofton, Miroslav N Nenov, Yafang Zhang, Federico Scala, Sean A Page, David L McCue, Dingge Li, Jonathan D Hommel, Fernanda Laezza, Thomas A Green
Psychiatric disorders such as anxiety, depression and addiction are often comorbid brain pathologies thought to share common mechanistic biology. As part of the cortico-limbic circuit, the nucleus accumbens shell (NAcSh) plays a fundamental role in integrating information in the circuit, such that modulation of NAcSh circuitry alters anxiety, depression, and addiction-related behaviors. Intracellular kinase cascades in the NAcSh have proven important mediators of behavior. To investigate glycogen-synthase kinase 3 (GSK3) beta signaling in the NAcSh in vivo we knocked down GSK3beta expression with a novel adeno-associated viral vector (AAV2) and assessed changes in anxiety- and depression-like behavior and cocaine self-administration in GSK3beta knockdown rats...
May 1, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28119060/conditional-knockout-of-fgf13-in-murine-hearts-increases-arrhythmia-susceptibility-and-reveals-novel-ion-channel-modulatory-roles
#19
Xiangchong Wang, He Tang, Eric Q Wei, Zhihua Wang, Jing Yang, Rong Yang, Sheng Wang, Yongjian Zhang, Geoffrey S Pitt, Hailin Zhang, Chuan Wang
The intracellular fibroblast growth factors (iFGF/FHFs) bind directly to cardiac voltage gated Na(+) channels, and modulate their function. Mutations that affect iFGF/FHF-Na(+) channel interaction are associated with arrhythmia syndromes. Although suspected to modulate other ionic currents, such as Ca(2+) channels based on acute knockdown experiments in isolated cardiomyocytes, the in vivo consequences of iFGF/FHF gene ablation on cardiac electrical activity are still unknown. We generated inducible, cardiomyocyte-restricted Fgf13 knockout mice to determine the resultant effects of Fgf13 gene ablation...
January 21, 2017: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/28116546/cortical-synaptic-transmission-and-plasticity-in-acute-liver-failure-are-decreased-by-presynaptic-events
#20
Mariusz Popek, Bartosz Bobula, Joanna Sowa, Grzegorz Hess, Rafał Polowy, Robert Kuba Filipkowski, Małgorzata Frontczak-Baniewicz, Barbara Zabłocka, Jan Albrecht, Magdalena Zielińska
Neurological symptoms of acute liver failure (ALF) reflect decreased excitatory transmission, but the status of ALF-affected excitatory synapse has not been characterized in detail. We studied the effects of ALF in mouse on synaptic transmission and plasticity ex vivo and its relation to distribution of (i) synaptic vesicles (sv) and (ii) functional synaptic proteins within the synapse. ALF-competent neurological and biochemical changes were induced in mice with azoxymethane (AOM). Electrophysiological characteristics (long-term potentiation, whole-cell recording) as well as synapse ultrastructure were evaluated in the cerebral cortex...
January 23, 2017: Molecular Neurobiology
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