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in vivo whole cell recording

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https://www.readbyqxmd.com/read/28809399/evidence-for-newly-generated-interneurons-in-the-basolateral-amygdala-of-adult-mice
#1
D J Jhaveri, A Tedoldi, S Hunt, R Sullivan, N R Watts, J M Power, P F Bartlett, P Sah
New neurons are continually generated from the resident populations of precursor cells in selective niches of the adult mammalian brain such as the hippocampal dentate gyrus and the olfactory bulb. However, whether such cells are present in the adult amygdala, and their neurogenic capacity, is not known. Using the neurosphere assay, we demonstrate that a small number of precursor cells, the majority of which express Achaete-scute complex homolog 1 (Ascl1), are present in the basolateral amygdala (BLA) of the adult mouse...
August 15, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28807302/targeted-overexpression-of-cyclic-amp-dependent-protein-kinase-subunit-in-toxoplasma-gondii-promotes-replication-and-virulence-in-host-cells
#2
Hongchao Sun, Suhua Wang, Xianfeng Zhao, Chaoqun Yao, Haohan Zhuang, Yechuan Huang, Xueqiu Chen, Yi Yang, Aifang Du
Toxoplasma gondii (T. gondii) is one of the most common parasite that can infect almost any warm-blooded animals including humans. The cyclic nucleotide-dependent protein kinase (PKA) regulates a spectrum of intracellular signal pathways in many organisms. Protein kinase catalytic subunit (PKAC) is the core of the whole protein, and plays an important role in the life cycle of T.gondii. Here, T.gondii PKAC (TgPKAC) overexpression strain (TgPKAC-OE) was constructed. The growth of the TgPKAC-OE, RH△Ku80, and TgPKAC inhibition strains (TgPKAC-H89) were analysed by SYBR-green real-time PCR, and the ultrastructure was observed by transmission electron microscopy...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28791701/electrophysiological-properties-of-basal-forebrain-cholinergic-neurons-identified-by-genetic-and-optogenetic-tagging
#3
Gretchen Y López-Hernández, Mala Ananth, Li Jiang, Elizabeth C Ballinger, David A Talmage, Lorna W Role
Recent developments in the generation of neuronal population-specific, genetically modified mouse lines have allowed precise identification and selective stimulation of cholinergic neurons in vivo. Although considerably less laborious than studies conducted with post hoc identification of cholinergic neurons by immunostaining, it is not known whether the genetically based labeling procedures that permit in vivo identification are electrophysiologically benign. In this study, we use mice carrying a bacterial artificial chromosome transgene that drives expression of a tau-green fluorescent fusion protein specifically in cholinergic neurons...
August 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28762595/perinatal-high-fat-diet-increases-inhibition-of-dorsal-motor-nucleus-of-the-vagus-neurons-regulating-gastric-functions
#4
C A McMenamin, R A Travagli, K N Browning
BACKGROUND: Previous studies suggest an increased inhibition of dorsal motor nucleus of the vagus (DMV) neurons following exposure to a perinatal high fat diet (PNHFD); the underlying neural mechanisms, however, remain unknown. This study assessed the effects of PNHFD on inhibitory synaptic inputs to DMV neurons and the vagally dependent control of gastric tone and motility. METHODS: Whole-cell patch clamp recordings were made from DMV neurons in thin brainstem slices from Sprague-Dawley rats fed either a control diet or HFD (14 or 60% kcal from fat, respectively) from embryonic day 13 onwards; gastric tone and motility were recorded in in vivo anesthetized rats...
August 1, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28760662/acute-ethanol-modulation-of-neurocircuit-function-in-the-nucleus-of-the-tractus-solitarius
#5
Michael A Aimino, Caitlin Coker, Yuval Silberman
The nucleus of the tractus solitarius (NTS) is a brain stem region critical to many physiologic processes and has been implicated in addiction to multiple classes of abused drugs, including alcohol (EtOH). That said, the mechanism by which EtOH modulates NTS neurocircuit activity is not well characterized and has yet to be examined utilizing electrophysiologic methods in mouse models of alcohol use disorders. To begin to address this gap in knowledge, we sought to use whole-cell and cell-attached recordings to determine the mechanism of acute EtOH action on GABAergic and glutamatergic neurotransmission, as well as on action potential firing in the NTS of adult male, EtOH naïve mice...
July 28, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/28729445/preferential-disruption-of-prefrontal-gabaergic-function-by-nanomolar-concentrations-of-the-%C3%AE-7nach-negative-modulator-kynurenic-acid
#6
Eden Flores-Barrera, Daniel R Thomases, Daryn K Cass, Ajay Bhandari, Robert Schwarcz, John P Bruno, Kuei Y Tseng
Increased concentrations of kynurenic acid (KYNA) in the prefrontal cortex (PFC) are thought to contribute to the development of cognitive deficits observed in schizophrenia. Although this view is consistent with pre-clinical studies showing a negative impact of prefrontal KYNA elevation on executive function, the mechanism underlying such a disruption remains unclear. Here we measured changes in local field potential responses to ventral hippocampal stimulation in vivo, and conducted whole-cell patch-clamp recordings in brain slices to reveal how nanomolar concentrations of KYNA alter synaptic transmission in the PFC of male adult rats...
July 20, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28689982/network-level-control-of-frequency-tuning-in-auditory-cortex
#7
Hiroyuki K Kato, Samuel K Asinof, Jeffry S Isaacson
Lateral inhibition is a fundamental circuit operation that sharpens the tuning properties of cortical neurons. This operation is classically attributed to an increase in GABAergic synaptic input triggered by non-preferred stimuli. Here we use in vivo whole-cell recording and two-photon Ca(2+) imaging in awake mice to show that lateral inhibition shapes frequency tuning in primary auditory cortex via an unconventional mechanism: non-preferred tones suppress both excitatory and inhibitory synaptic inputs onto layer 2/3 cells ("network suppression")...
July 19, 2017: Neuron
https://www.readbyqxmd.com/read/28678348/nacl-and-osmolarity-produce-different-responses-in-organum-vasculosum-of-the-lamina-terminalis-neurons-sympathetic-nerve-activity-and-blood-pressure
#8
Brian J Kinsman, Kirsteen N Browning, Sean D Stocker
Systemic or central infusion of hypertonic NaCl and other osmolytes readily stimulate thirst and vasopressin secretion. In contrast, central infusion of hypertonic NaCl produces a greater increase in arterial blood pressure (ABP) than equi-osmotic mannitol/sorbitol. Although these responses depend on neurons in the organum vasculosum of the lamina terminalis (OVLT), these observations suggest OVLT neurons may sense or respond differently to hypertonic NaCl versus osmolarity. The purpose of this study was to test this hypothesis in Sprague-Dawley rats...
July 5, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28664138/naringenin-inhibits-pacemaking-activity-in-interstitial-cells-of-cajal-from-murine-small-intestine
#9
Hyun Jung Kim, Byung Joo Kim
BACKGROUND: Naringenin (NRG) is a common dietary polyphenolic constituent of fruits. NRG has diverse pharmacological activities, and is used in traditional medicine to treat various diseases including gastrointestinal (GI) disorders. Interstitial cells of Cajal (ICCs) are pacemaker cells of the GI tract. In this study, the authors investigated the effects of NRG on ICCs and on GI motility in vitro and in vivo. METHODS: ICCs were dissociated from mouse small intestines by enzymatic digestion...
June 2017: Integrative medicine research
https://www.readbyqxmd.com/read/28660248/optogenetic-evidence-for-a-direct-circuit-linking-nociceptive-transmission-through-the-parabrachial-complex-with-pain-modulating-neurons-of-the-rostral-ventromedial-medulla-rvm
#10
QiLiang Chen, Zachary Roeder, Ming-Hua Li, YangMiao Zhang, Susan L Ingram, Mary M Heinricher
The parabrachial complex (PB) is a functionally and anatomically complex structure involved in a range of homeostatic and sensory functions, including nociceptive transmission. There is also evidence that PB can engage descending pain-modulating systems, the best characterized of which is the rostral ventromedial medulla (RVM). Two distinct classes of RVM neurons, "ON-cells" and "OFF-cells," exert net pronociceptive and anti-nociceptive effects, respectively. PB was recently shown to be a relay of nociceptive information to RVM ON- and OFF-cells...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28660247/phototransduction-in-drosophila-is-compromised-by-gal4-expression-but-not-by-insp3-receptor-knockdown-or-mutation
#11
Murali K Bollepalli, Marije E Kuipers, Che-Hsiung Liu, Sabrina Asteriti, Roger C Hardie
Drosophila phototransduction is mediated by phospholipase C, leading to activation of transient receptor potential (TRP) and TRP-like (TRPL) channels by mechanisms that are unresolved. A role for InsP3 receptors (IP3Rs) had been excluded because IP3R mutants (itpr) appeared to have normal light responses; however, this was recently challenged by Kohn et al. ("Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo," Journal of Neuroscience 35:2530), who reported defects in phototransduction after IP3R-RNAi knockdown...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28654039/electrophysiological-method-for-whole-cell-voltage-clamp-recordings-from-drosophila-photoreceptors
#12
Ben Katz, Rita Gutorov, Elisheva Rhodes-Mordov, Roger C Hardie, Baruch Minke
Whole-cell voltage clamp recordings from Drosophila melanogaster photoreceptors have revolutionized the field of invertebrate visual transduction, enabling the use of D. melanogaster molecular genetics to study inositol-lipid signaling and Transient Receptor Potential (TRP) channels at the single-molecule level. A handful of labs have mastered this powerful technique, which enables the analysis of the physiological responses to light under highly controlled conditions. This technique allows control over the intracellular and extracellular media; the membrane voltage; and the fast application of pharmacological compounds, such as a variety of ionic or pH indicators, to the intra- and extracellular media...
June 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28646418/in-vivo-nmr-as-a-tool-for-probing-molecular-structure-and-dynamics-in-intact-chlamydomonas-reinhardtii-cells
#13
Fatemeh Azadi-Chegeni, Christo Schiphorst, Anjali Pandit
We report the application of NMR dynamic spectral editing for probing the structure and dynamics of molecular constituents in fresh, intact cells and in freshly prepared thylakoid membranes of Chlamydomonas reinhardtii (Cr.) green algae. For isotope labeling, wild-type Cr. cells were grown on (13)C acetate-enriched minimal medium. 1D (13)C J-coupling based and dipolar-based MAS NMR spectra were applied to distinguish (13)C resonances of different molecular components. 1D spectra were recorded over a physiological temperature range, and whole-cell spectra were compared to those taken from thylakoid membranes, evaluating their composition and dynamics...
June 23, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28640864/targeting-breast-cancer-cells-by-mrs1477-a-positive-allosteric-modulator-of-trpv1-channels
#14
Mustafa Nazıroğlu, Bilal Çiğ, Walter Blum, Csaba Vizler, Andrea Buhala, Annamária Marton, Róbert Katona, Katalin Jósvay, Beat Schwaller, Zoltán Oláh, László Pecze
There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive allosteric modulator of TRPV1, if added together with capsaicin, but is ineffective, if given alone. Addition of MRS1477 evoked Ca2+ signals in MCF7 breast cancer cells, but not in primary breast epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28592699/lower-affinity-of-isradipine-for-l-type-ca-2-channels-during-substantia-nigra-dopamine-neuron-like-activity-implications-for-neuroprotection-in-parkinson-s-disease
#15
Nadine J Ortner, Gabriella Bock, Antonios Dougalis, Maria Kharitonova, Johanna Duda, Simon Hess, Petronel Tuluc, Thomas Pomberger, Nadia Stefanova, Florian Pitterl, Thomas Ciossek, Herbert Oberacher, Henning J Draheim, Peter Kloppenburg, Birgit Liss, Jörg Striessnig
Ca(2+)-influx through L-type Ca(2+)-channels (LTCCs) is associated with activity-related stressful oscillations of Ca(2+) levels within dopaminergic (DA) neurons in the substantia nigra (SN), which may contribute to their selective degeneration in Parkinson's disease (PD). LTCC blockers were neuroprotective in mouse neurotoxin models of PD, and isradipine is currently undergoing testing in a phase III clinical trial in early PD. We report no evidence for neuroprotection by in vivo pretreatment with therapeutically relevant isradipine plasma levels, or Cav1...
July 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28592686/adaptation-modulates-correlated-subthreshold-response-variability-in-visual-cortex
#16
Nathaniel C Wright, Mahmood S Hoseini, Ralf Wessel
Cortical sensory responses are highly variable across stimulus presentations. This variability can be correlated across neurons (due to some combination of dense intracortical connectivity, cortical activity level, and cortical state), with fundamental implications for population coding. Yet the interpretation of correlated response variability (or "noise correlation") has remained fraught with difficulty, in part because of the restriction to extracellular neuronal spike recordings. Here, we measured response variability and its correlation at the most microscopic level of electrical neural activity, the membrane potential, by obtaining dual whole cell recordings from pairs of cortical pyramidal neurons during visual processing in the turtle whole brain ex vivo preparation...
August 1, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28592685/robotic-navigation-to-subcortical-neural-tissue-for-intracellular-electrophysiology-in-vivo
#17
W A Stoy, I Kolb, G L Holst, Y Liew, A Pala, B Yang, E S Boyden, G B Stanley, C R Forest
In vivo studies of neurophysiology using the whole cell patch-clamp technique enable exquisite access to both intracellular dynamics and cytosol of cells in the living brain but are underrepresented in deep subcortical nuclei because of fouling of the sensitive electrode tip. We have developed an autonomous method to navigate electrodes around obstacles such as blood vessels after identifying them as a source of contamination during regional pipette localization (RPL) in vivo. In mice, robotic navigation prevented fouling of the electrode tip, increasing RPL success probability 3 mm below the pial surface to 82% (n = 72/88) over traditional, linear localization (25%, n = 24/95), and resulted in high-quality thalamic whole cell recordings with average access resistance (32...
August 1, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28591742/andrographolide-inhibits-arrhythmias-and-is-cardioprotective-in-rabbits
#18
Mengliu Zeng, Wanzhen Jiang, Youjia Tian, Jie Hao, Zhenzhen Cao, Zhipei Liu, Chen Fu, Peihua Zhang, Jihua Ma
Andrographolide has a protective effect on the cardiovascular system. To study its cardic-electrophysiological effects, action potentials and voltage-gated Na+ (INa), Ca2+ (ICaL), and K+ (IK1, IKr, Ito and IKur) currents were recorded using whole-cell patch clamp and current clamp techniques. Additionally, the effects of andrographolide on aconitine-induced arrhythmias were assessed on electrocardiograms in vivo. We found that andrographolide shortened action potential duration and reduced maximum upstroke velocity in rabbit left ventricular and left atrial myocytes...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28576943/polysialic-acid-regulates-sympathetic-outflow-by-facilitating-information-transfer-within-the-nucleus-of-the-solitary-tract
#19
Phillip Bokiniec, Shila Shahbazian, Stuart J McDougall, Britt A Berning, Delfine Cheng, Ida J Llewellyn-Smith, Peter Gr Burke, Simon McMullan, Martina Mühlenhoff, Herbert Hildebrandt, Filip Braet, Mark Connor, Nicolle H Packer, Ann K Goodchild
Expression of the large, extracellular glycan, polysialic acid (polySia) is restricted, in the adult, to brain regions exhibiting high levels of plasticity or remodelling, including the hippocampus, prefrontal cortex and the nucleus of the solitary tract (NTS). The NTS, located in the dorsal brainstem, receives constant viscerosensory afferent traffic as well as input from central regions controlling sympathetic nerve activity, respiration, gastrointestinal functions, hormonal release and behaviour. Our aims were to determine the ultrastructural location of polySia in the NTS and the functional effects of enzymatic removal of polySia, both in vitro and in vivo PolySia immunoreactivity was found throughout the adult rat NTS...
June 2, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28576617/synthesis-of-resveratrol-derivatives-as-new-analgesic-drugs-through-desensitization-of-the-trpa1-receptor
#20
Syuhei Nakao, Miyuki Mabuchi, Shenglan Wang, Yoko Kogure, Tadashi Shimizu, Koichi Noguchi, Akito Tanaka, Yi Dai
A series of 31 resveratrol derivatives was designed, synthesized and evaluated for activation and inhibition of the TRPA1 channel. Most acted as activators and desensitizers of TRPA1 channels like resveratrol or allyl isothiocyanate (AITC). Compound 4z (HUHS029) exhibited higher inhibitory activity than resveratrol with an IC50 value of 16.1μM. The activity of 4z on TRPA1 was confirmed in TRPA1-expressing HEK293 cells, as well as in rat dorsal root ganglia neurons by a whole cell patch clamp recording. Furthermore, pretreatment with 4z exhibited an analgesic effect on AITC-evoked TRPA1-related pain behavior in vivo...
May 22, 2017: Bioorganic & Medicinal Chemistry Letters
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