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in vivo whole cell recording

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https://www.readbyqxmd.com/read/28932197/antiplasmodial-activity-and-toxicological-assessment-of-curcumin-plga-encapsulated-nanoparticles
#1
Zulaikha A Busari, Kabiru A Dauda, Olajumoke A Morenikeji, Funmilayo Afolayan, Oyetunde T Oyeyemi, Jairam Meena, Debasis Sahu, Amulya K Panda
Curcumin is a polyphenolic pigment isolated from the rhizomes of Curcuma longa (turmeric), a medicinal plant widely used in the ancient Indian and Chinese medicine. The antiplasmodial activity of curcumin is often hampered by its fast metabolism and poor water solubility, thus its incorporation into a delivery system could circumvent this problem. This study aimed to evaluate the in vivo antiplasmodial activity and the toxicity assessment of curcumin incorporated into poly (lactic-co-glycolic) acid (PLGA) nanoparticles...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28931571/sublaminar-subdivision-of-mouse-auditory-cortex-layer-2-3-based-on-functional-translaminar-connections
#2
Xiangying Meng, Daniel E Winkowski, Joseph P Y Kao, Patrick O Kanold
The cerebral cortex is subdivided into 6 layers based on morphological features. The supragranular layers 2/3 (L2/3) contain morphologically and genetically diverse populations of neurons, suggesting the existence of discrete classes of cells. In primates and carnivores L2/3 can be subdivided morphologically, but cytoarchitectonic divisions are less clear in rodents. Nevertheless, discrete classes of cells could exist based on their computational requirement, which might be linked to their associated functional microcircuits...
September 20, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28910617/whole-cell-recording-of-neuronal-membrane-potential-during-behavior
#3
REVIEW
Carl C H Petersen
Neuronal membrane potential is of fundamental importance for the mechanistic understanding of brain function. This review discusses progress in whole-cell patch-clamp recordings for low-noise measurement of neuronal membrane potential in awake behaving animals. Whole-cell recordings can be combined with two-photon microscopy to target fluorescently labeled neurons, revealing cell-type-specific membrane potential dynamics of retrogradely or genetically labeled neurons. Dual whole-cell recordings reveal behavioral modulation of membrane potential synchrony and properties of synaptic transmission in vivo...
September 13, 2017: Neuron
https://www.readbyqxmd.com/read/28878623/mechanisms-of-spontaneous-climbing-fiber-discharge-evoked-pauses-and-output-modulation-of-cerebellar-purkinje-cell-in-mice
#4
Xian-Hua Jin, Hong-Wei Wang, Xin-Yuan Zhang, Chun-Ping Chu, Yuan-Zhe Jin, Song-Biao Cui, De-Lai Qiu
Climbing fiber (CF) afferents modulate the frequency and patterns of cerebellar Purkinje cell (PC) simple spike (SS) activity, but its mechanism is unclear. In the present study, we investigated the mechanisms of spontaneous CF discharge-evoked pauses and the output modulation of cerebellar PCs in urethane-anesthetized mice using in vivo whole-cell recording techniques and pharmacological methods. Under voltage-clamp recording conditions, spontaneous CF discharge evoked strong inward currents followed by small conductance calcium-activated potassium (SK) channels that mediated outward currents...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28870570/effects-of-naftopidil-on-inhibitory-transmission-in-substantia-gelatinosa-neurons-of-the-rat-spinal-dorsal-horn-in-vitro
#5
Daisuke Uta, Du-Jie Xie, Tsuyoshi Hattori, Ken-Ichi Kasahara, Megumu Yoshimura
OBJECTIVE: Naftopidil is used clinically for the treatment of voiding disorders in benign prostatic hyperplasia. Previous in vivo experiments in which naftopidil was applied intrathecally abolished rhythmic bladder contraction, suggesting that naftopidil might inhibit a voiding reflex through interaction with spinal dorsal horn neurons. Here we aimed to clarify the mechanism of action of naftopidil on dorsal horn neurons. METHODS: Whole-cell patch-clamp recordings were performed using substantia gelatinosa neurons of adult rat spinal cord slices...
September 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28858615/robotic-automation-of-in%C3%A2-vivo-two-photon-targeted-whole-cell-patch-clamp-electrophysiology
#6
Luca A Annecchino, Alexander R Morris, Caroline S Copeland, Oshiorenoya E Agabi, Paul Chadderton, Simon R Schultz
Whole-cell patch-clamp electrophysiological recording is a powerful technique for studying cellular function. While in vivo patch-clamp recording has recently benefited from automation, it is normally performed "blind," meaning that throughput for sampling some genetically or morphologically defined cell types is unacceptably low. One solution to this problem is to use two-photon microscopy to target fluorescently labeled neurons. Combining this with robotic automation is difficult, however, as micropipette penetration induces tissue deformation, moving target cells from their initial location...
August 30, 2017: Neuron
https://www.readbyqxmd.com/read/28844004/altered-intrinsic-and-synaptic-properties-of-lumbosacral-dorsal-horn-neurons-in-a-mouse-model-of-colitis
#7
Kristen E Farrell, Simon Keely, Marjorie M Walker, Alan M Brichta, Brett A Graham, Robert J Callister
Visceral pain in inflammatory and functional gastrointestinal conditions is a major clinical problem. The exact mechanisms underlying the development of pain, during and after visceral inflammation are unknown. However, clinical and pre-clinical evidence suggests plasticity within the spinal cord dorsal horn is a contributing factor. Here we use an in vivo preparation and patch-clamp electrophysiology to test whether the synaptic and intrinsic properties of superficial dorsal horn (SDH) neurons are altered 5days after the induction of mild colitis in adult male mice (i...
August 23, 2017: Neuroscience
https://www.readbyqxmd.com/read/28837632/green-fluorescent-genetically-encoded-calcium-indicator-based-on-calmodulin-m13-peptide-from-fungi
#8
Natalia V Barykina, Oksana M Subach, Kiryl D Piatkevich, Erica E Jung, Aleksey Y Malyshev, Ivan V Smirnov, Andrey O Bogorodskiy, Valentin I Borshchevskiy, Anna M Varizhuk, Galina E Pozmogova, Edward S Boyden, Konstantin V Anokhin, Grigori N Enikolopov, Fedor V Subach
Currently available genetically encoded calcium indicators (GECIs) utilize calmodulins (CaMs) or troponin C from metazoa such as mammals, birds, and teleosts, as calcium-binding domains. The amino acid sequences of the metazoan calcium-binding domains are highly conserved, which may limit the range of the GECI key parameters and cause undesired interactions with the intracellular environment in mammalian cells. Here we have used fungi, evolutionary distinct organisms, to derive CaM and its binding partner domains and design new GECI with improved properties...
2017: PloS One
https://www.readbyqxmd.com/read/28809399/evidence-for-newly-generated-interneurons-in-the-basolateral-amygdala-of-adult-mice
#9
D J Jhaveri, A Tedoldi, S Hunt, R Sullivan, N R Watts, J M Power, P F Bartlett, P Sah
New neurons are continually generated from the resident populations of precursor cells in selective niches of the adult mammalian brain such as the hippocampal dentate gyrus and the olfactory bulb. However, whether such cells are present in the adult amygdala, and their neurogenic capacity, is not known. Using the neurosphere assay, we demonstrate that a small number of precursor cells, the majority of which express Achaete-scute complex homolog 1 (Ascl1), are present in the basolateral amygdala (BLA) of the adult mouse...
August 15, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28807302/targeted-overexpression-of-cyclic-amp-dependent-protein-kinase-subunit-in-toxoplasma-gondii-promotes-replication-and-virulence-in-host-cells
#10
Hongchao Sun, Suhua Wang, Xianfeng Zhao, Chaoqun Yao, Haohan Zhuang, Yechuan Huang, Xueqiu Chen, Yi Yang, Aifang Du
Toxoplasma gondii (T. gondii) is one of the most common parasite that can infect almost any warm-blooded animals including humans. The cyclic nucleotide-dependent protein kinase (PKA) regulates a spectrum of intracellular signal pathways in many organisms. Protein kinase catalytic subunit (PKAC) is the core of the whole protein, and plays an important role in the life cycle of T.gondii. Here, T.gondii PKAC (TgPKAC) overexpression strain (TgPKAC-OE) was constructed. The growth of the TgPKAC-OE, RH△Ku80, and TgPKAC inhibition strains (TgPKAC-H89) were analysed by SYBR-green real-time PCR, and the ultrastructure was observed by transmission electron microscopy...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28791701/electrophysiological-properties-of-basal-forebrain-cholinergic-neurons-identified-by-genetic-and-optogenetic-tagging
#11
Gretchen Y López-Hernández, Mala Ananth, Li Jiang, Elizabeth C Ballinger, David A Talmage, Lorna W Role
Recent developments in the generation of neuronal population-specific, genetically modified mouse lines have allowed precise identification and selective stimulation of cholinergic neurons in vivo. Although considerably less laborious than studies conducted with post hoc identification of cholinergic neurons by immunostaining, it is not known whether the genetically based labeling procedures that permit in vivo identification are electrophysiologically benign. In this study, we use mice carrying a bacterial artificial chromosome transgene that drives expression of a tau-green fluorescent fusion protein specifically in cholinergic neurons...
August 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28762595/perinatal-high-fat-diet-increases-inhibition-of-dorsal-motor-nucleus-of-the-vagus-neurons-regulating-gastric-functions
#12
C A McMenamin, R A Travagli, K N Browning
BACKGROUND: Previous studies suggest an increased inhibition of dorsal motor nucleus of the vagus (DMV) neurons following exposure to a perinatal high fat diet (PNHFD); the underlying neural mechanisms, however, remain unknown. This study assessed the effects of PNHFD on inhibitory synaptic inputs to DMV neurons and the vagally dependent control of gastric tone and motility. METHODS: Whole-cell patch clamp recordings were made from DMV neurons in thin brainstem slices from Sprague-Dawley rats fed either a control diet or HFD (14 or 60% kcal from fat, respectively) from embryonic day 13 onwards; gastric tone and motility were recorded in in vivo anesthetized rats...
August 1, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28760662/acute-ethanol-modulation-of-neurocircuit-function-in-the-nucleus-of-the-tractus-solitarius
#13
Michael A Aimino, Caitlin R Coker, Yuval Silberman
The nucleus of the tractus solitarius (NTS) is a brain stem region critical to many physiologic processes and has been implicated in addiction to multiple classes of abused drugs, including alcohol (EtOH). That said, the mechanism by which EtOH modulates NTS neurocircuit activity is not well characterized and has yet to be examined utilizing electrophysiologic methods in mouse models of alcohol use disorders. To begin to address this gap in knowledge, we sought to use whole-cell and cell-attached recordings to determine the mechanism of acute EtOH action on GABAergic and glutamatergic neurotransmission, as well as on action potential firing in the NTS of adult male, EtOH naïve mice...
July 29, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/28729445/preferential-disruption-of-prefrontal-gabaergic-function-by-nanomolar-concentrations-of-the-%C3%AE-7nach-negative-modulator-kynurenic-acid
#14
Eden Flores-Barrera, Daniel R Thomases, Daryn K Cass, Ajay Bhandari, Robert Schwarcz, John P Bruno, Kuei Y Tseng
Increased concentrations of kynurenic acid (KYNA) in the prefrontal cortex (PFC) are thought to contribute to the development of cognitive deficits observed in schizophrenia. Although this view is consistent with preclinical studies showing a negative impact of prefrontal KYNA elevation on executive function, the mechanism underlying such a disruption remains unclear. Here, we measured changes in local field potential (LFP) responses to ventral hippocampal stimulation in vivo and conducted whole-cell patch-clamp recordings in brain slices to reveal how nanomolar concentrations of KYNA alter synaptic transmission in the PFC of male adult rats...
August 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28689982/network-level-control-of-frequency-tuning-in-auditory-cortex
#15
Hiroyuki K Kato, Samuel K Asinof, Jeffry S Isaacson
Lateral inhibition is a fundamental circuit operation that sharpens the tuning properties of cortical neurons. This operation is classically attributed to an increase in GABAergic synaptic input triggered by non-preferred stimuli. Here we use in vivo whole-cell recording and two-photon Ca(2+) imaging in awake mice to show that lateral inhibition shapes frequency tuning in primary auditory cortex via an unconventional mechanism: non-preferred tones suppress both excitatory and inhibitory synaptic inputs onto layer 2/3 cells ("network suppression")...
July 19, 2017: Neuron
https://www.readbyqxmd.com/read/28678348/nacl-and-osmolarity-produce-different-responses-in-organum-vasculosum-of-the-lamina-terminalis-neurons-sympathetic-nerve-activity-and-blood-pressure
#16
Brian J Kinsman, Kirsteen N Browning, Sean D Stocker
KEY POINTS: Changes in extracellular osmolarity stimulate thirst and vasopressin secretion through a central osmoreceptor; however, central infusion of hypertonic NaCl produces a greater sympathoexcitatory and pressor response than infusion of hypertonic mannitol/sorbitol. Neurons in the organum vasculosum of the lamina terminalis (OVLT) sense changes in extracellular osmolarity and NaCl. In this study, we discovered that intracerebroventricular infusion or local OVLT injection of hypertonic NaCl increases lumbar sympathetic nerve activity, adrenal sympathetic nerve activity and arterial blood pressure whereas equi-osmotic mannitol/sorbitol did not alter any variable...
September 15, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28664138/naringenin-inhibits-pacemaking-activity-in-interstitial-cells-of-cajal-from-murine-small-intestine
#17
Hyun Jung Kim, Byung Joo Kim
BACKGROUND: Naringenin (NRG) is a common dietary polyphenolic constituent of fruits. NRG has diverse pharmacological activities, and is used in traditional medicine to treat various diseases including gastrointestinal (GI) disorders. Interstitial cells of Cajal (ICCs) are pacemaker cells of the GI tract. In this study, the authors investigated the effects of NRG on ICCs and on GI motility in vitro and in vivo. METHODS: ICCs were dissociated from mouse small intestines by enzymatic digestion...
June 2017: Integrative medicine research
https://www.readbyqxmd.com/read/28660248/optogenetic-evidence-for-a-direct-circuit-linking-nociceptive-transmission-through-the-parabrachial-complex-with-pain-modulating-neurons-of-the-rostral-ventromedial-medulla-rvm
#18
QiLiang Chen, Zachary Roeder, Ming-Hua Li, YangMiao Zhang, Susan L Ingram, Mary M Heinricher
The parabrachial complex (PB) is a functionally and anatomically complex structure involved in a range of homeostatic and sensory functions, including nociceptive transmission. There is also evidence that PB can engage descending pain-modulating systems, the best characterized of which is the rostral ventromedial medulla (RVM). Two distinct classes of RVM neurons, "ON-cells" and "OFF-cells," exert net pronociceptive and anti-nociceptive effects, respectively. PB was recently shown to be a relay of nociceptive information to RVM ON- and OFF-cells...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28660247/phototransduction-in-drosophila-is-compromised-by-gal4-expression-but-not-by-insp3-receptor-knockdown-or-mutation
#19
Murali K Bollepalli, Marije E Kuipers, Che-Hsiung Liu, Sabrina Asteriti, Roger C Hardie
Drosophila phototransduction is mediated by phospholipase C, leading to activation of transient receptor potential (TRP) and TRP-like (TRPL) channels by mechanisms that are unresolved. A role for InsP3 receptors (IP3Rs) had been excluded because IP3R mutants (itpr) appeared to have normal light responses; however, this was recently challenged by Kohn et al. ("Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo," Journal of Neuroscience 35:2530), who reported defects in phototransduction after IP3R-RNAi knockdown...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28654039/electrophysiological-method-for-whole-cell-voltage-clamp-recordings-from-drosophila-photoreceptors
#20
Ben Katz, Rita Gutorov, Elisheva Rhodes-Mordov, Roger C Hardie, Baruch Minke
Whole-cell voltage clamp recordings from Drosophila melanogaster photoreceptors have revolutionized the field of invertebrate visual transduction, enabling the use of D. melanogaster molecular genetics to study inositol-lipid signaling and Transient Receptor Potential (TRP) channels at the single-molecule level. A handful of labs have mastered this powerful technique, which enables the analysis of the physiological responses to light under highly controlled conditions. This technique allows control over the intracellular and extracellular media; the membrane voltage; and the fast application of pharmacological compounds, such as a variety of ionic or pH indicators, to the intra- and extracellular media...
June 13, 2017: Journal of Visualized Experiments: JoVE
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