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in vivo whole cell recording

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https://www.readbyqxmd.com/read/29121216/fast-inhibitory-decay-facilitates-adult-like-temporal-processing-in-layer-5-of-developing-primary-auditory-cortex
#1
Fenghua Xie, Ling You, Dongqin Cai, Miaomiao Liu, Yin Yue, Yiwei Wang, Kexin Yuan
The protracted maturational process of temporal processing in layer 4 (L4) of primary auditory cortex (A1) has been extensively studied. Accumulating evidences show that layer 5 (L5) receives direct thalamic inputs as well. How the temporal responses in L5 may developmentally emerge remains unclear. Using in vivo loose-patch recordings in rat A1, we found that putative pyramidal (Pyr) neurons in developing L5 exhibited adult-like stimulus-following ability but less bursting shortly after hearing onset. L5 Pyr neurons in adult A1 exhibited phase-locking similar to L4 neurons, while L5 fast-spiking (FS) neurons showed greater phase-locking at 7 and 12...
October 31, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/29109420/passage-dependent-accumulation-of-somatic-mutations-in-mesenchymal-stromal-cells-during-in-vitro-culture-revealed-by-whole-genome-sequencing
#2
Myungshin Kim, Je-Keun Rhee, Hayoung Choi, Ahlm Kwon, Jiyeon Kim, Gun Dong Lee, Dong Wook Jekarl, Seungok Lee, Yonggoo Kim, Tae-Min Kim
Human mesenchymal stromal cells (MSCs) have served as a major cellular resource for cell-based immunomodulatory and regenerative therapies. However, genomic instability may accumulate during ex vivo expansion of MSCs, thereby increasing the potential of malignant transformation. Here, we performed whole genome sequencing of two peripheral blood-derived MSC lines (MSC1 and MSC2) at various passages (passage 1 [P1] to P9). The majority of single-nucleotide variations (SNVs) occurred in later passages; specifically, 90% and 70% of all SNVs in MSC1 and MSC2 were observed in P9 and P7/P9, respectively...
November 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29094727/a-pivotal-role-for-the-activation-of-trpv3-channel-in-itch-sensations-induced-by-the-natural-skin-sensitizer-carvacrol
#3
Ting-Ting Cui, Gong-Xin Wang, Ning-Ning Wei, KeWei Wang
Itching is an intricate, common symptom of dermatologic and systemic diseases, and both TRPV3 and TRPA1 channels have been suggested to function as downstream effector targets. But the relative contributions of TRPV3 and TRPA1 to itch sensation in vivo remain unclear. To dissect the role of TRPA1 or TRPV3 in the cutaneous sensation of itching, we took the advantage of a natural compound carvacrol from oregano, and examined its effect on the induction of scratching behavior in mice. We showed that the intradermal injection of carvacrol (0...
November 2, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/29093172/a-computational-analysis-of-signal-fidelity-in-the-rostral-nucleus-of-the-solitary-tract
#4
Alison Boxwell, David Terman, Marion Frank, Yuchio Yanagawa, Joseph B Travers
Neurons in the rostral nucleus of the solitary tract (rNST) convey taste information to both local circuits and pathways destined for forebrain structures. This nucleus is more than a simple relay, however, since rNST neurons differ in response rates and tuning curves relative to primary afferent fibers. To systematically study the impact of convergence and inhibition on firing frequency and breadth of tuning (BOT) in rNST, we constructed a mathematical model of its two major cell types: projection neurons and inhibitory neurons...
November 1, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/29069619/breakdown-of-excitability-by-attenuated-prv-152-infection-in-auditory-brainstem-neurons-of-mongolian-gerbils
#5
Christian P Porres, Benedikt Grothe, Felix Felmy
Pseudorabies virus (PRV), a neurovirulent α-herpesvirus, spreads between neurons at synaptic connections. PRV-infected neurons have been shown to exhibit functional deficits with the attenuated PRV152 Bartha strain negatively influencing neuronal functioning in in vitro model systems. However, the impact of this attenuated PRV152 Bartha strain on the native central nervous system has not been fully explored. Using a combination of in vivo stereotactic injections and post-hoc in vitro whole-cell recordings, we investigated the functional impact of PRV152 Bartha in the auditory system of juvenile Mongolian gerbils...
October 22, 2017: Neuroscience
https://www.readbyqxmd.com/read/29066971/the-sigma-2-receptor-selective-agonist-siramesine-lu-28-179-decreases-cocaine-reinforced-pavlovian-learning-and-alters-glutamatergic-and-dopaminergic-input-to-the-striatum
#6
Anna M Klawonn, Anna Nilsson, Carl F Rådberg, Sarah H Lindström, Mia Ericson, Björn Granseth, David Engblom, Michael Fritz
Drug addiction is a chronic, debilitating disease that affects millions of people around the world causing a substantial societal burden. Despite decades of research efforts, treatment possibilities remain limited and relapse represents the most treatment-resistant element. Neurosteroid sigma-1 receptors have been meticulously studied in psychostimulant reinforced Pavlovian learning, while the sigma-2 receptor subtype has remained unexplored. Recent development of selective sigma-2 receptor ligands have now made it possible to investigate if the sigma-2 receptor system is a potential target to treat drug addiction...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29055091/functional-interaction-of-junctophilin-2-with-small-conductance-ca-2-activated-potassium-channel-subtype-2-sk2-in-mouse-cardiac-myocytes
#7
Hong K Fan, Tian X Luo, Wei D Zhao, Yong H Mu, Yang Yang, Wen J Guo, Hui Y Tu, Qian Zhang
AIM: Junctophilins (JPs), a protein family of the junctional membrane complex, maintain the close conjunction between cell surface and intracellular membranes in striate muscle cells mediating the cross talk between extracellular Ca(2+) entry and intracellular Ca(2+) release. The small conductance Ca(2+) -activated K(+) channels are activated by the intracellular calcium and play an essential role in the cardiac action potential profile. Molecular mechanisms of regulation of the SK channels are still uncertain...
October 21, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/29049988/magnolia-officinalis-bark-extract-induces-depolarization-of-pacemaker-potentials-through-m2-and-m3-muscarinic-receptors-in-cultured-murine-small-intestine-interstitial-cells-of-cajal
#8
Hyun Jung Kim, Taewon Han, Yun Tai Kim, Insuk So, Byung Joo Kim
BACKGROUND: Magnolia officinalis Rehder and EH Wilson (M. officinalis) are traditional Chinese medicines widely used for gastrointestinal (GI) tract motility disorder in Asian countries. We investigated the effects of an ethanol extract of M. officinalis (MOE) on the pacemaker potentials of cultured interstitial cells of Cajal (ICCs) in vitro and its effects on GI motor functions in vivo. METHODS: We isolated ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record the pacemaker potentials in cultured ICCs in vitro...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29033834/tc299423-a-novel-agonist-for-nicotinic-acetylcholine-receptors
#9
Teagan R Wall, Brandon J Henderson, George Voren, Charles R Wageman, Purnima Deshpande, Bruce N Cohen, Sharon R Grady, Michael J Marks, Daniel Yohannes, Paul J Kenny, Merouane Bencherif, Henry A Lester
(E)-5-(Pyrimidin-5-yl)-1,2,3,4,7,8-hexahydroazocine (TC299423) is a novel agonist for nicotinic acetylcholine receptors (nAChRs). We examined its efficacy, affinity, and potency for α6β2(∗) (α6β2-containing), α4β2(∗), and α3β4(∗) nAChRs, using [(125)I]-epibatidine binding, whole-cell patch-clamp recordings, synaptosomal (86)Rb(+) efflux, [(3)H]-dopamine release, and [(3)H]-acetylcholine release. TC299423 displayed an EC50 of 30-60 nM for α6β2(∗) nAChRs in patch-clamp recordings and [(3)H]-dopamine release assays...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29031852/inhibition-of-cdk5-rejuvenates-inhibitory-circuits-and-restores-experience-dependent-plasticity-in-adult-visual-cortex
#10
Yue Li, Laijian Wang, Xinxin Zhang, Mengyao Huang, Sitong Li, Xinxing Wang, Lin Chen, Bin Jiang, Yupeng Yang
Cyclin-dependent kinase 5 (Cdk5) acts as an essential modulator for neural development and neurological disorders. Here we show that Cdk5 plays a pivotal role in modulating GABAergic signaling and the maturation of visual system. In adult mouse primary visual cortex, Cdk5 formed complex with the GABA synthetic enzyme glutamate decarboxylase GAD67, but not with GAD65. In addition to enhancement in the surface level of NR2B-containing NMDA receptors, inhibition of Cdk5 reduced the protein levels of GADs and Otx2, while leaving intact the expression of vesicular GABA transporter and subunits of GABAA or AMPA receptors...
October 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29024712/brief-activation-of-gabaergic-interneurons-initiates-the-transition-to-ictal-events-through-post-inhibitory-rebound-excitation
#11
Michael Chang, Joshua A Dian, Suzie Dufour, Lihua Wang, Homeira Moradi Chameh, Meera Ramani, Liang Zhang, Peter L Carlen, Thilo Womelsdorf, Taufik A Valiante
Activation of γ-aminobutyric acid (GABAA) receptors have been associated with the onset of epileptiform events. To investigate if a causal relationship exists between GABAA receptor activation and ictal event onset, we activated inhibitory GABAergic networks in the superficial layer (2/3) of the somatosensory cortex during hyperexcitable conditions using optogenetic techniques in mice expressing channelrhodopsin-2 in all GABAergic interneurons. We found that a brief 30ms light pulse reliably triggered either an interictal-like event (IIE) or ictal-like ("ictal") event in the in vitro cortical 4-Aminopyridine (4-AP) slice model...
October 10, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/29020623/functional-plasticity-of-odor-representations-during-motherhood
#12
Amit Vinograd, Yael Fuchs-Shlomai, Merav Stern, Diptendu Mukherjee, Yuan Gao, Ami Citri, Ian Davison, Adi Mizrahi
Motherhood is accompanied by new behaviors aimed at ensuring the wellbeing of the offspring. Olfaction plays a key role in guiding maternal behaviors during this transition. We studied functional changes in the main olfactory bulb (OB) of mothers in mice. Using in vivo two-photon calcium imaging, we studied the sensory representation of odors by mitral cells (MCs). We show that MC responses to monomolecular odors become sparser and weaker in mothers. In contrast, responses to biologically relevant odors are spared from sparsening or strengthen...
October 10, 2017: Cell Reports
https://www.readbyqxmd.com/read/29018970/andersen-s-syndrome-mutants-produce-a-knockdown-of-inwardly-rectifying-k-channel-in-mouse-skeletal-muscle-in-vivo
#13
Dina Simkin, Gaëlle Robin, Serena Giuliano, Ana Vukolic, Pamela Moceri, Nicolas Guy, Kay-Dietrich Wagner, Alain Lacampagne, Bruno Allard, Saïd Bendahhou
Andersen's syndrome (AS) is a rare autosomal disorder that has been defined by the triad of periodic paralysis, cardiac arrhythmia, and developmental anomalies. AS has been directly linked to over 40 different autosomal dominant negative loss-of-function mutations in the KCNJ2 gene, encoding for the tetrameric strong inward rectifying K(+) channel KIR2.1. While KIR2.1 channels have been suggested to contribute to setting the resting membrane potential (RMP) and to control the duration of the action potential (AP) in skeletal and cardiac muscle, the mechanism by which AS mutations produce such complex pathophysiological symptoms is poorly understood...
October 10, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28988851/ablation-of-c-fibers-decreases-quantal-size-of-gabaergic-synaptic-transmission-in-the-insular-cortex
#14
Shota Murayama, Kiyofumi Yamamoto, Mari Kaneko, Bunnai Ogiso, Masayuki Kobayashi
The primary sensory cortex exhibits neuroplastic changes responding to sensory disturbances, and GABAergic synaptic transmission plays a critical role in the regulation of plasticity. The insular cortex (IC) integrates orofacial nociceptive signals conveyed via myelinated Aδ- and unmyelinated C-fibers. However, it has been unknown whether a disturbance of nociceptive inputs, such as a deletion of the peripheral nerves, alters GABAergic local circuit in IC. The present study elucidated GABAergic synaptic transmission in the model rat whose C-fibers were ablated by capsaicin injection 1-2 days after birth...
December 4, 2017: Neuroscience
https://www.readbyqxmd.com/read/28981586/gabapentin-prevents-progressive-increases-in-excitatory-connectivity-and-epileptogenesis-following-neocortical-trauma
#15
D K Takahashi, Sha Jin, D A Prince
Neocortical injury initiates a cascade of events, some of which result in maladaptive epileptogenic reorganization of surviving neural circuits. Research focused on molecular and organizational changes that occur following trauma may reveal processes that underlie human post-traumatic epilepsy (PTE), a common and unfortunate consequence of traumatic brain injury. The latency between injury and development of PTE provides an opportunity for prophylactic intervention, once the key underlying mechanisms are understood...
June 26, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28977859/andrographolide-inhibits-arrhythmias-and-is-cardioprotective-in-rabbits
#16
Mengliu Zeng, Wanzhen Jiang, Youjia Tian, Jie Hao, Zhenzhen Cao, Zhipei Liu, Chen Fu, Peihua Zhang, Jihua Ma
Andrographolide has a protective effect on the cardiovascular system. To study its cardic-electrophysiological effects, action potentials and voltage-gated Na(+) (INa), Ca(2+) (ICaL), and K(+) (IK1, IKr, Ito and IKur) currents were recorded using whole-cell patch clamp and current clamp techniques. Additionally, the effects of andrographolide on aconitine-induced arrhythmias were assessed on electrocardiograms in vivo. We found that andrographolide shortened action potential duration and reduced maximum upstroke velocity in rabbit left ventricular and left atrial myocytes...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28943120/diversity-of-bilateral-synaptic-assemblies-for-binaural-computation-in-midbrain-single-neurons
#17
Na He, Lingzhi Kong, Tao Lin, Shaohui Wang, Xiuping Liu, Jiyao Qi, Jun Yan
Binaural hearing confers many beneficial functions but our understanding of its underlying neural substrates is limited. This study examines the bilateral synaptic assemblies and binaural computation (or integration) in the central nucleus of the inferior colliculus (ICc) of the auditory midbrain, a key convergent center. Using in-vivo whole-cell patch-clamp, the excitatory and inhibitory postsynaptic potentials (EPSPs/IPSPs) of single ICc neurons to contralateral, ipsilateral and bilateral stimulation were recorded...
September 18, 2017: Hearing Research
https://www.readbyqxmd.com/read/28932197/antiplasmodial-activity-and-toxicological-assessment-of-curcumin-plga-encapsulated-nanoparticles
#18
Zulaikha A Busari, Kabiru A Dauda, Olajumoke A Morenikeji, Funmilayo Afolayan, Oyetunde T Oyeyemi, Jairam Meena, Debasis Sahu, Amulya K Panda
Curcumin is a polyphenolic pigment isolated from the rhizomes of Curcuma longa (turmeric), a medicinal plant widely used in the ancient Indian and Chinese medicine. The antiplasmodial activity of curcumin is often hampered by its fast metabolism and poor water solubility, thus its incorporation into a delivery system could circumvent this problem. This study aimed to evaluate the in vivo antiplasmodial activity and the toxicity assessment of curcumin incorporated into poly (lactic-co-glycolic) acid (PLGA) nanoparticles...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28931571/sublaminar-subdivision-of-mouse-auditory-cortex-layer-2-3-based-on-functional-translaminar-connections
#19
Xiangying Meng, Daniel E Winkowski, Joseph P Y Kao, Patrick O Kanold
The cerebral cortex is subdivided into six layers based on morphological features. The supragranular layers 2/3 (L2/3) contain morphologically and genetically diverse populations of neurons, suggesting the existence of discrete classes of cells. In primates and carnivores L2/3 can be subdivided morphologically, but cytoarchitectonic divisions are less clear in rodents. Nevertheless, discrete classes of cells could exist based on their computational requirement, which might be linked to their associated functional microcircuits...
October 18, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28910617/whole-cell-recording-of-neuronal-membrane-potential-during-behavior
#20
REVIEW
Carl C H Petersen
Neuronal membrane potential is of fundamental importance for the mechanistic understanding of brain function. This review discusses progress in whole-cell patch-clamp recordings for low-noise measurement of neuronal membrane potential in awake behaving animals. Whole-cell recordings can be combined with two-photon microscopy to target fluorescently labeled neurons, revealing cell-type-specific membrane potential dynamics of retrogradely or genetically labeled neurons. Dual whole-cell recordings reveal behavioral modulation of membrane potential synchrony and properties of synaptic transmission in vivo...
September 13, 2017: Neuron
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