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in vivo whole cell recording

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https://www.readbyqxmd.com/read/28689982/network-level-control-of-frequency-tuning-in-auditory-cortex
#1
Hiroyuki K Kato, Samuel K Asinof, Jeffry S Isaacson
Lateral inhibition is a fundamental circuit operation that sharpens the tuning properties of cortical neurons. This operation is classically attributed to an increase in GABAergic synaptic input triggered by non-preferred stimuli. Here we use in vivo whole-cell recording and two-photon Ca(2+) imaging in awake mice to show that lateral inhibition shapes frequency tuning in primary auditory cortex via an unconventional mechanism: non-preferred tones suppress both excitatory and inhibitory synaptic inputs onto layer 2/3 cells ("network suppression")...
July 5, 2017: Neuron
https://www.readbyqxmd.com/read/28678348/nacl-and-osmolarity-produce-different-responses-in-organum-vasculosum-of-the-lamina-terminalis-neurons-sympathetic-nerve-activity-and-blood-pressure
#2
Brian J Kinsman, Kirsteen N Browning, Sean D Stocker
Systemic or central infusion of hypertonic NaCl and other osmolytes readily stimulate thirst and vasopressin secretion. In contrast, central infusion of hypertonic NaCl produces a greater increase in arterial blood pressure (ABP) than equi-osmotic mannitol/sorbitol. Although these responses depend on neurons in the organum vasculosum of the lamina terminalis (OVLT), these observations suggest OVLT neurons may sense or respond differently to hypertonic NaCl versus osmolarity. The purpose of this study was to test this hypothesis in Sprague-Dawley rats...
July 5, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28664138/naringenin-inhibits-pacemaking-activity-in-interstitial-cells-of-cajal-from-murine-small-intestine
#3
Hyun Jung Kim, Byung Joo Kim
BACKGROUND: Naringenin (NRG) is a common dietary polyphenolic constituent of fruits. NRG has diverse pharmacological activities, and is used in traditional medicine to treat various diseases including gastrointestinal (GI) disorders. Interstitial cells of Cajal (ICCs) are pacemaker cells of the GI tract. In this study, the authors investigated the effects of NRG on ICCs and on GI motility in vitro and in vivo. METHODS: ICCs were dissociated from mouse small intestines by enzymatic digestion...
June 2017: Integrative medicine research
https://www.readbyqxmd.com/read/28660248/optogenetic-evidence-for-a-direct-circuit-linking-nociceptive-transmission-through-the-parabrachial-complex-with-pain-modulating-neurons-of-the-rostral-ventromedial-medulla-rvm
#4
QiLiang Chen, Zachary Roeder, Ming-Hua Li, YangMiao Zhang, Susan L Ingram, Mary M Heinricher
The parabrachial complex (PB) is a functionally and anatomically complex structure involved in a range of homeostatic and sensory functions, including nociceptive transmission. There is also evidence that PB can engage descending pain-modulating systems, the best characterized of which is the rostral ventromedial medulla (RVM). Two distinct classes of RVM neurons, "ON-cells" and "OFF-cells," exert net pronociceptive and anti-nociceptive effects, respectively. PB was recently shown to be a relay of nociceptive information to RVM ON- and OFF-cells...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28660247/phototransduction-in-drosophila-is-compromised-by-gal4-expression-but-not-by-insp3-receptor-knockdown-or-mutation
#5
Murali K Bollepalli, Marije E Kuipers, Che-Hsiung Liu, Sabrina Asteriti, Roger C Hardie
Drosophila phototransduction is mediated by phospholipase C, leading to activation of transient receptor potential (TRP) and TRP-like (TRPL) channels by mechanisms that are unresolved. A role for InsP3 receptors (IP3Rs) had been excluded because IP3R mutants (itpr) appeared to have normal light responses; however, this was recently challenged by Kohn et al. ("Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo," Journal of Neuroscience 35:2530), who reported defects in phototransduction after IP3R-RNAi knockdown...
May 2017: ENeuro
https://www.readbyqxmd.com/read/28654039/electrophysiological-method-for-whole-cell-voltage-clamp-recordings-from-drosophila-photoreceptors
#6
Ben Katz, Rita Gutorov, Elisheva Rhodes-Mordov, Roger C Hardie, Baruch Minke
Whole-cell voltage clamp recordings from Drosophila melanogaster photoreceptors have revolutionized the field of invertebrate visual transduction, enabling the use of D. melanogaster molecular genetics to study inositol-lipid signaling and Transient Receptor Potential (TRP) channels at the single-molecule level. A handful of labs have mastered this powerful technique, which enables the analysis of the physiological responses to light under highly controlled conditions. This technique allows control over the intracellular and extracellular media; the membrane voltage; and the fast application of pharmacological compounds, such as a variety of ionic or pH indicators, to the intra- and extracellular media...
June 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28646418/in-vivo-nmr-as-a-tool-for-probing-molecular-structure-and-dynamics-in-intact-chlamydomonas-reinhardtii-cells
#7
Fatemeh Azadi-Chegeni, Christo Schiphorst, Anjali Pandit
We report the application of NMR dynamic spectral editing for probing the structure and dynamics of molecular constituents in fresh, intact cells and in freshly prepared thylakoid membranes of Chlamydomonas reinhardtii (Cr.) green algae. For isotope labeling, wild-type Cr. cells were grown on (13)C acetate-enriched minimal medium. 1D (13)C J-coupling based and dipolar-based MAS NMR spectra were applied to distinguish (13)C resonances of different molecular components. 1D spectra were recorded over a physiological temperature range, and whole-cell spectra were compared to those taken from thylakoid membranes, evaluating their composition and dynamics...
June 23, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28640864/targeting-breast-cancer-cells-by-mrs1477-a-positive-allosteric-modulator-of-trpv1-channels
#8
Mustafa Nazıroğlu, Bilal Çiğ, Walter Blum, Csaba Vizler, Andrea Buhala, Annamária Marton, Róbert Katona, Katalin Jósvay, Beat Schwaller, Zoltán Oláh, László Pecze
There is convincing epidemiological and experimental evidence that capsaicin, a potent natural transient receptor potential cation channel vanilloid member 1 (TRPV1) agonist, has anticancer activity. However, capsaicin cannot be given systemically in large doses, because of its induction of acute pain and neurological inflammation. MRS1477, a dihydropyridine derivative acts as a positive allosteric modulator of TRPV1, if added together with capsaicin, but is ineffective, if given alone. Addition of MRS1477 evoked Ca2+ signals in MCF7 breast cancer cells, but not in primary breast epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28592699/lower-affinity-of-isradipine-for-l-type-ca-2-channels-during-substantia-nigra-dopamine-neuron-like-activity-implications-for-neuroprotection-in-parkinson-s-disease
#9
Nadine J Ortner, Gabriella Bock, Antonios Dougalis, Maria Kharitonova, Johanna Duda, Simon Hess, Petronel Tuluc, Thomas Pomberger, Nadia Stefanova, Florian Pitterl, Thomas Ciossek, Herbert Oberacher, Henning J Draheim, Peter Kloppenburg, Birgit Liss, Jörg Striessnig
Ca(2+)-influx through L-type Ca(2+)-channels (LTCCs) is associated with activity-related stressful oscillations of Ca(2+) levels within dopaminergic (DA) neurons in the substantia nigra (SN), which may contribute to their selective degeneration in Parkinson's disease (PD). LTCC blockers were neuroprotective in mouse neurotoxin models of PD, and isradipine is currently undergoing testing in a phase III clinical trial in early PD. We report no evidence for neuroprotection by in vivo pretreatment with therapeutically relevant isradipine plasma levels, or Cav1...
July 12, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28592686/adaptation-modulates-correlated-subthreshold-response-variability-in-visual-cortex
#10
Nathaniel Caleb Wright, Mahmood Sayed Hoseini, Ralf Wessel
Cortical sensory responses are highly variable across stimulus presentations. This variability can be correlated across neurons (due to some combination of dense intracortical connectivity, cortical activity level, and cortical state), with fundamental implications for population coding. Yet the interpretation of correlated response variability (or "noise correlation") has remained fraught with difficulty, in part because of the restriction to extracellular neuronal spike recordings. Here, we measured response variability and its correlation at the most microscopic level of electrical neural activity, the membrane potential, by obtaining dual whole-cell recordings from pairs of cortical pyramidal neurons during visual processing in the turtle whole-brain ex vivo preparation...
June 7, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28592685/robotic-navigation-to-sub-cortical-neural-tissue-for-intracellular-electrophysiology-in-vivo
#11
William Andrew Stoy, Ilya Kolb, Gregory Holst, Yi J Liew, Aurelie Pala, Bo Yang, Edward S Boyden, Garrett B Stanley, Craig R Forest
In vivo studies of neurophysiology using the whole-cell patch clamp technique enable exquisite access to both intracellular dynamics and cytosol of cells in the living brain but are underrepresented in deep subcortical nuclei due to fouling of the sensitive electrode tip. We have developed an autonomous method to navigate electrodes around obstacles such as blood vessels, after identifying them as a source of contamination during regional pipette localization (RPL) in vivo. In mice, robotic navigation prevented fouling of the electrode tip, increasing RPL success probability 3 mm below the pial surface to 82% (n=72/88) over traditional, linear localization (25%, n=24/95) and resulted in high quality thalamic whole-cell recordings with average access resistance (32...
June 7, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28591742/andrographolide-inhibits-arrhythmias-and-is-cardioprotective-in-rabbits
#12
Mengliu Zeng, Wanzhen Jiang, Youjia Tian, Jie Hao, Zhenzhen Cao, Zhipei Liu, Chen Fu, Peihua Zhang, Jihua Ma
Andrographolide has a protective effect on the cardiovascular system. To study its cardic-electrophysiological effects, action potentials and voltage-gated Na+ (INa), Ca2+ (ICaL), and K+ (IK1, IKr, Ito and IKur) currents were recorded using whole-cell patch clamp and current clamp techniques. Additionally, the effects of andrographolide on aconitine-induced arrhythmias were assessed on electrocardiograms in vivo. We found that andrographolide shortened action potential duration and reduced maximum upstroke velocity in rabbit left ventricular and left atrial myocytes...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28576943/polysialic-acid-regulates-sympathetic-outflow-by-facilitating-information-transfer-within-the-nucleus-of-the-solitary-tract
#13
Phillip Bokiniec, Shila Shahbazian, Stuart J McDougall, Britt A Berning, Delfine Cheng, Ida J Llewellyn-Smith, Peter Gr Burke, Simon McMullan, Martina Mühlenhoff, Herbert Hildebrandt, Filip Braet, Mark Connor, Nicolle H Packer, Ann K Goodchild
Expression of the large, extracellular glycan, polysialic acid (polySia) is restricted, in the adult, to brain regions exhibiting high levels of plasticity or remodelling, including the hippocampus, prefrontal cortex and the nucleus of the solitary tract (NTS). The NTS, located in the dorsal brainstem, receives constant viscerosensory afferent traffic as well as input from central regions controlling sympathetic nerve activity, respiration, gastrointestinal functions, hormonal release and behaviour. Our aims were to determine the ultrastructural location of polySia in the NTS and the functional effects of enzymatic removal of polySia, both in vitro and in vivo PolySia immunoreactivity was found throughout the adult rat NTS...
June 2, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28576617/synthesis-of-resveratrol-derivatives-as-new-analgesic-drugs-through-desensitization-of-the-trpa1-receptor
#14
Syuhei Nakao, Miyuki Mabuchi, Shenglan Wang, Yoko Kogure, Tadashi Shimizu, Koichi Noguchi, Akito Tanaka, Yi Dai
A series of 31 resveratrol derivatives was designed, synthesized and evaluated for activation and inhibition of the TRPA1 channel. Most acted as activators and desensitizers of TRPA1 channels like resveratrol or allyl isothiocyanate (AITC). Compound 4z (HUHS029) exhibited higher inhibitory activity than resveratrol with an IC50 value of 16.1μM. The activity of 4z on TRPA1 was confirmed in TRPA1-expressing HEK293 cells, as well as in rat dorsal root ganglia neurons by a whole cell patch clamp recording. Furthermore, pretreatment with 4z exhibited an analgesic effect on AITC-evoked TRPA1-related pain behavior in vivo...
May 22, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28566460/mesoscale-duration-activated-states-gate-spiking-in-response-to-fast-rises-in-membrane-voltage-in-the-awake-brain
#15
Annabelle C Singer, Giovanni Talei Franzesi, Suhasa B Kodandaramaiah, Francisco J Flores, Jeremy D Cohen, Albert K Lee, Christoph Börgers, Craig R Forest, Nancy J Kopell, Edward S Boyden
Seconds-scale network states, affecting many neurons within a network, modulate neural activity by complementing fast integration of neuron-specific inputs that arrive in the milliseconds before spiking. Non-rhythmic subthreshold dynamics at intermediate timescales, however, are less well-characterized. We found, using automated whole cell patch clamping in vivo, that spikes recorded in CA1 and barrel cortex in awake mice are often preceded not only by monotonic voltage rises lasting milliseconds, but also by more gradual (lasting 10s-100s of ms) depolarizations...
May 31, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28555875/enhancement-of-synchronized-activity-between-hippocampal-ca1-neurons-during-initial-storage-of-associative-fear-memory
#16
Yu-Zhang Liu, Yao Wang, Weida Shen, Zhiru Wang
The hippocampus is critical for memory acquisition and consolidation. This function requires activity- and experience-induced neuronal plasticity. It is known that neuronal plasticity is largely dependent on synchronized activity. As has been well characterized, repetitive correlated activity of presynaptic and postsynaptic neurons can lead to long-term modifications at their synapses. Studies on network activity have also suggested that memory processing in the hippocampus may involve learning-induced changes of neuronal synchronization, as observed in vivo between hippocampal CA3 and CA1 networks as well as between the rhinal cortex and the hippocampus...
May 26, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28542001/acetaminophen-metabolite-n-acylphenolamine-induces-analgesia-via-transient-receptor-potential-vanilloid-1-receptors-expressed-on-the-primary-afferent-terminals-of-c-fibers-in-the-spinal-dorsal-horn
#17
Nobuko Ohashi, Daisuke Uta, Mika Sasaki, Masayuki Ohashi, Yoshinori Kamiya, Tatsuro Kohno
BACKGROUND: The widely used analgesic acetaminophen is metabolized to N-acylphenolamine, which induces analgesia by acting directly on transient receptor potential vanilloid 1 or cannabinoid 1 receptors in the brain. Although these receptors are also abundant in the spinal cord, no previous studies have reported analgesic effects of acetaminophen or N-acylphenolamine mediated by the spinal cord dorsal horn. We hypothesized that clinical doses of acetaminophen induce analgesia via these spinal mechanisms...
August 2017: Anesthesiology
https://www.readbyqxmd.com/read/28521136/dopamine-depletion-impairs-bilateral-sensory-processing-in-the-striatum-in-a-pathway-dependent-manner
#18
Maya Ketzef, Giada Spigolon, Yvonne Johansson, Alessandra Bonito-Oliva, Gilberto Fisone, Gilad Silberberg
Parkinson's disease (PD) is a movement disorder caused by the loss of dopaminergic innervation, particularly to the striatum. PD patients often exhibit sensory impairments, yet the underlying network mechanisms are unknown. Here we examined how dopamine (DA) depletion affects sensory processing in the mouse striatum. We used the optopatcher for online identification of direct and indirect pathway projection neurons (MSNs) during in vivo whole-cell recordings. In control mice, MSNs encoded the laterality of sensory inputs with larger and earlier responses to contralateral than ipsilateral whisker deflection...
May 17, 2017: Neuron
https://www.readbyqxmd.com/read/28498898/spatial-asymmetry-and-short-term-suppression-underlie-direction-selectivity-of-synaptic-excitation-in-the-mouse-visual-cortex
#19
Ya-Tang Li, Qi Fang, Li I Zhang, Huizhong Whit Tao
Direction selectivity (DS) of neuronal responses is fundamental for motion detection. With in vivo whole-cell voltage-clamp recordings from layer (L)4 neurons in the mouse visual cortex, we observed a strong correlation between DS and spatial asymmetry in the distribution of excitatory input strengths. This raises an interesting possibility that the latter may contribute to DS. The preferred direction of excitatory input was found from the stronger to weaker side of its spatial receptive field. A simple linear summation of asymmetrically distributed excitatory responses to stationary flash stimuli however failed to predict the correct directionality: it at best resulted in weak DS with preferred direction opposite to what was observed experimentally...
May 11, 2017: Cerebral Cortex
https://www.readbyqxmd.com/read/28495603/ethnopharmacological-uses-phytochemistry-biological-activities-and-therapeutic-applications-of-clinacanthus-nutans-burm-f-lindau-a-comprehensive-review
#20
REVIEW
Muhamad Noor Alfarizal Kamarudin, Md Moklesur Rahman Sarker, Habsah Abdul Kadir, Long Chiau Ming
ETHNOPHARMACOLOGICAL RELEVANCE: Clinacanthus nutans (Burm. f.) Lindau, a widely used medicinal plant, is extensively grown in tropical Asia and Southeast Asian countries. C. nutans, with its broad spectrum of pharmacological activities, has been traditionally used to treat cancer, inflammatory disorders, diabetes, insect bites, and skin problems, consumed as a vegetable, mixed with fresh juices, in concoctions, and as a whole plant. The present review analyzes the advances in the ethnopharmacology, phytochemistry, pharmacology, and toxicology of C...
July 12, 2017: Journal of Ethnopharmacology
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