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https://www.readbyqxmd.com/read/28109583/detection-of-recombinant-human-lactoferrin-and-lysozyme-produced-in-a-bitransgenic-cow
#1
Germán G Kaiser, Nicolás C Mucci, Vega González, Lourdes Sánchez, José A Parrón, María D Pérez, Miguel Calvo, Juan F Aller, Federico A Hozbor, Adrián A Mutto
Lactoferrin and lysozyme are 2 glycoproteins with great antimicrobial activity, being part of the nonspecific defensive system of human milk, though their use in commercial products is difficult because human milk is a limited source. Therefore, many investigations have been carried out to produce those proteins in biological systems, such as bacteria, yeasts, or plants. Mammals seem to be more suitable as expression systems for human proteins, however, especially for those that are glycosylated. In the present study, a bicistronic commercial vector containing a goat β-casein promoter and an internal ribosome entry site fragment between the human lactoferrin and human lysozyme genes was developed to allow the introduction of both genes into bovine adult fibroblasts in a single transfection...
January 18, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28109272/opinions-about-the-new-law-on-end-of-life-issues-in-a-sample-of-french-patients-receiving-palliative-care
#2
Augustin Boulanger, Théo Chabal, Marie Fichaux, Mireille Destandau, Jean Marc La Piana, Pascal Auquier, Karine Baumstarck, Sébastien Salas
BACKGROUND: In February 2nd 2016, the French government enacted the Claeys-Leonetti law that forbade euthanasia and established the right to deep and continuous sedation for end-of-life patients. Moreover, the law also obliges clinicians to abide by any advance directives regarding treatment and investigation, except in cases where they are "obviously inappropriate" in a given medical situation, or in cases of emergency, in order to allow medical staff to take time to assess the patient's situation...
January 21, 2017: BMC Palliative Care
https://www.readbyqxmd.com/read/28095908/limiting-treatment-and-shortening-of-life-data-from-a-cross-sectional-survey-in-germany-on-frequencies-determinants-and-patients-involvement
#3
Birte Malena Dahmen, Jochen Vollmann, Stephan Nadolny, Jan Schildmann
BACKGROUND: Limiting treatment forms part of practice in many fields of medicine. There is a scarcity of robust data from Germany. Therefore, in this paper, we report results of a survey among German physicians with a focus on frequencies, aspects of decision making and determinants of limiting treatment with expected or intended shortening of life. METHODS: Postal survey among a random sample of physicians working in the area of five German state chambers of physicians using a modified version of the questionnaire of the EURELD Consortium...
January 17, 2017: BMC Palliative Care
https://www.readbyqxmd.com/read/28095077/running-performance-with-nutritive-and-non-nutritive-sweetened-mouth-rinses
#4
Keely H Hawkins, Sridevi Krishnan, Lara Ringos, Vanessa Garcia, Jamie A Cooper
: Mouth rinsing (MR) with carbohydrate during exercise has been shown to act as an ergogenic aid. PURPOSE: To investigate if nutritive or nonnutritive sweetened MR affect exercise performance, and to assess the influence of sweetness intensity on endurance performance during a time-trial (TT). METHODS: This randomized, single blinded study had 4 treatment conditions. 16 subjects (9 men, 7 women) completed a 12.8km TT four different times. During each TT, subjects MR and expectorated a different solution at time 0 and every 12...
January 17, 2017: International Journal of Sports Physiology and Performance
https://www.readbyqxmd.com/read/28092151/issues-in-nutrition-carbohydrates
#5
Margaret E Thompson, Mary Barth Noel
Carbohydrates include sugars, starches, and dietary fibers. Resistant starches resemble fiber in their behavior in the intestinal tract, and may have positive effects on blood glucose levels and the gut microbiome. Fibers are classified as soluble and insoluble, but most fiber-containing foods contain a mixture of soluble and insoluble fiber. Soluble fiber has been shown to lower low-density lipoprotein cholesterol levels. Many artificial sweeteners and other sugar substitutes are available. Most natural sources of sweeteners also are energy sources...
January 2017: FP Essentials
https://www.readbyqxmd.com/read/28074217/-nutrition-in-dementia
#6
D Volkert
BACKGROUND: In the course of dementia sooner or later nutritional problems appear, and the question arises which interventions are effective in ensuring adequate nutrition and thus may contribute to the maintenance of health, functionality and independence. OBJECTIVE: This overview presents the state of knowledge regarding nutrition in dementia. METHODS: This work is based on the present guidelines of the European Society for Clinical Nutrition and Metabolism (ESPEN) on nutrition in dementia, which systematically investigated relevant available evidence...
January 10, 2017: Der Internist
https://www.readbyqxmd.com/read/28041869/-palliative-care-after-neonatal-intensive-care-contributions-of-leonetti-law-and-remaining-challenges
#7
P Kuhn, L Dillenseger, N Cojean, B Escande, C Zores, D Astruc
The 2005 enactment of the "Patients' rights and end-of-life care" act, known as the Leonetti law, has been accompanied by practical changes in the processes of withdrawal and withholding of active life-sustaining treatments. This law has also promoted the implementation of palliative care in perinatal medicine to avoid unreasonable therapeutic interventions and to preserve the dying patient's quality of life and human dignity. Recently, a new law has been voted by the French National Assembly and new reflections on the ethical aspects of the end of life in neonatal medicine should resume again within the French Society of Neonatology in the working group on ethical issues in neonatology...
December 29, 2016: Archives de Pédiatrie: Organe Officiel de la Sociéte Française de Pédiatrie
https://www.readbyqxmd.com/read/28032354/end-of-life-treatment-decisions-in-nursing-home-residents-dying-with-dementia-in-the-netherlands
#8
Simone A Hendriks, Martin Smalbrugge, Luc Deliens, Raymond T C M Koopmans, Bregje D Onwuteaka-Philipsen, Cees M P M Hertogh, Jenny T van der Steen
OBJECTIVE: The objective was to describe end-of-life treatment decisions for patients dying with dementia in various stages of dementia in long-term care facilities in the Netherlands with elderly care physicians responsible for treatment and care. METHODS: We present data collected in the nationally representative Dutch End of Life in Dementia study (2007-2011). Within 2 weeks after death, 103 physicians completed questionnaires about the last phase of life in 330 residents with dementia who resided in 1 of 34 participating long-term care facilities...
December 29, 2016: International Journal of Geriatric Psychiatry
https://www.readbyqxmd.com/read/28027797/corrigendum-to-espen-guidelines-on-chronic-intestinal-failure-in-adults-clin-nutr-35-2-2016-247-307
#9
Loris Pironi, Jann Arends, Federico Bozzetti, Cristina Cuerda, Lyn Gillanders, Palle Bekker Jeppesen, Francisca Joly, Darlene Kelly, Simon Lal, Michael Staun, Kinga Szczepanek, André Van Gossum, Geert Wanten, Stéphane Michel Schneider
No abstract text is available yet for this article.
December 23, 2016: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27997595/seedborne-pathogenic-fungi-in-common-bean-phaseolus-vulgaris-cv-inta-rojo-in-nicaragua
#10
Delfia Marcenaro, Jari P T Valkonen
Common bean (Phaseolus vulgaris L.) is an important legume with high nutritional value. In Nicaragua, certified healthy seeds of local bean varieties are not available, and seedborne fungi have gained little attention. Here, were surveyed seedborne pathogenic fungi in an important local bean cultivar, 'INTA Rojo'. Beans grown in the four main production areas in Nicaragua (Boaco, Carazo, Estelí, Matagalpa) for future use as seed stock were sampled from four seed storehouses and six seed lots. A total of 133 fungal strains were isolated from surface-sterilized beans and inoculated to healthy lima beans (Phaseolus lunatus) under controlled conditions...
2016: PloS One
https://www.readbyqxmd.com/read/27989563/preclinical-evidence-for-the-addiction-potential-of-highly-palatable-foods-current-developments-related-to-maternal-influence
#11
David A Wiss, Kristen Criscitelli, Mark Gold, Nicole Avena
It is well established that obesity has reached pandemic proportions. Over the last four decades the prevalence of obesity and morbid obesity have risen substantially in both men and women worldwide. Although there are many causative factors leading to excessive weight gain including genetics and sedentary lifestyle, the transformation of the food environment has undoubtedly contributed to the dangerously high rates of obesity. The current food landscape is inundated with food engineered to contain artificially high levels of sugar and fat...
December 15, 2016: Appetite
https://www.readbyqxmd.com/read/27979140/development-of-a-laboratory-technique-for-the-evaluation-of-protease-enzymes-activity-in-goat-and-sheep-milk
#12
Raffaele Palomba, Giorgio Formisano, Assunta Arrichiello, Giuseppe Auriemma, Fiorella Sarubbi
The shelf life of milk and its products is strongly influenced by the quality of raw milk. Several bacteria present in raw milk can produce proteases: among them, those from psychotropic microorganisms (e.g. Bacillus subtilis), which produce enzymes under refrigeration, cause the most serious problem. The interaction of these enzymes with the milk proteins and the main proteolytic enzymes becomes important in determining the quality of the milk. An artificial substrate (azocasein) was used to quantify the enzyme activity through the release of a chromogenic product that was measured spectrophotometrically after clarifying the sample by the addition of trichloroacetic acid...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27956737/effects-of-aspartame-monk-fruit-stevia-and-sucrose-sweetened-beverages-on-postprandial-glucose-insulin-and-energy-intake
#13
S L Tey, N B Salleh, J Henry, C G Forde
BackgroundSubstituting sweeteners with non-nutritive sweeteners (NNS) may aid in glycaemic control and body weight management. Limited studies have investigated energy compensation, glycaemic and insulinaemic responses to artificial and natural NNS.ObjectivesThis study compared the effects of consuming NNS (artificial vs. natural) and sucrose (65 g) on energy intake, blood glucose and insulin responses.MethodsThirty healthy males took part in this randomised, crossover study with four treatments: aspartame-, monk fruit-, Stevia-, and sucrose-sweetened beverages...
December 13, 2016: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27939312/caribbean-spiny-lobster-fishery-is-underpinned-by-trophic-subsidies-from-chemosynthetic-primary-production
#14
Nicholas D Higgs, Jason Newton, Martin J Attrill
The Caribbean spiny lobster, Panulirus argus, is one of the most valuable fisheries commodities in the Central American region, directly employing 50,000 people and generating >US$450 million per year [1]. This industry is particularly important to small island states such as The Bahamas, which exports more lobster than any other country in the region [1]. Several factors contribute to this disproportionally high productivity, principally the extensive shallow-water banks covered in seagrass meadows [2], where fishermen deploy artificial shelters for the lobsters to supplement scarce reef habitat [3]...
December 19, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27912013/sprouting-as-a-gardening-strategy-to-obtain-superior-supplementary-food-evidence-from-a-seed-caching-marine-worm
#15
Zhenchang Zhu, Jim van Belzen, Tao Hong, Tadao Kunihiro, Tom Ysebaert, Peter M J Herman, Tjeerd J Bouma
Only a handful of non-human animals are known to grow their own food by cultivating high-yield fungal or algal crops as staple food. Here we report an alternative strategy utilized by an omnivorous marine worm, Hediste diversicolor, to supplement its diet: gardening by sprouting seeds. In addition to having many other known feeding modes, we showed using video recordings and manipulative mesocosm experiments that this species can also behave like gardeners by deliberately burying cordgrass seeds in their burrows, which has been previously shown to reduce the loss of seeds to water...
December 2016: Ecology
https://www.readbyqxmd.com/read/27901038/burden-assessment-in-caregivers-of-patients-with-home-artificial-nutrition-a-need-and-a-challenge
#16
R Villar-Taibo, M A Martínez-Olmos, D Bellido-Guerrero, A Calleja-Fernández, R Peinó-García, A Martís-Sueiro, E Camarero-González, V Ríos-Barreiro, P Cao-Sánchez, R Durán-Martínez, M-J Rodríguez-Iglesias, B Rodríguez-Blanco, J Rojo-Valdés
BACKGROUND/OBJECTIVES: Caregiving can be a stressful task with severe consequences on caregivers' health. Our aim was to evaluate the profile and the burden of caregivers of patients with home artificial nutrition (HAN) in our area. SUBJECTS/METHODS: We conducted a prospective observational study of patients who had started HAN over a period of a year (n=573) and their home caregivers (n=103). Epidemiological characteristics of the patients and the type of HAN were registered...
November 30, 2016: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/27900726/survey-on-german-palliative-care-specialists-experiences-with-advance-directives
#17
Julia Zenz, Michael Zenz
INTRODUCTION: Advance directives (AD) play a central role in end-of-life treatments, intensive care, and palliative care. However, little is known about the experiences of healthcare professionals with ADs. This study reports on palliative care professionals' views on advance directives (AD). METHODS: A questionnaire was handed out to attendants of a palliative care symposium. RESULTS: Complete answers were obtained from 126 physicians and 276 nurses...
November 29, 2016: Pain and Therapy
https://www.readbyqxmd.com/read/27899911/pain-perception-in-unresponsive-wakefulness-syndrome-may-challenge-the-interruption-of-artificial-nutrition-and-hydration-neuroethics-in-action
#18
Francesco Riganello, Simone Macrì, Enrico Alleva, Carlo Petrini, Andrea Soddu, Josè Leòn-Carriòn, Giuliano Dolce
No abstract text is available yet for this article.
2016: Frontiers in Neurology
https://www.readbyqxmd.com/read/27893844/the-florida-harvester-ant-pogonomyrmex-badius-relies-on-germination-to-consume-large-seeds
#19
Walter R Tschinkel, Christina L Kwapich
The Florida harvester ant, Pogonomyrmex badius, is one of many ant species and genera that stores large numbers of seeds in damp, underground chambers for later consumption. A comparison of the sizes of seeds recovered from storage chambers with those of seed husks discarded following consumption revealed that the used seeds are far smaller than stored seeds. This difference in use-rate was confirmed in field and laboratory colonies by offering marked seeds of various sizes and monitoring the appearance of size-specific chaff...
2016: PloS One
https://www.readbyqxmd.com/read/27878797/choosing-wisely-at-the-end-of-life-the-crucial-role-of-medical-indication
#20
Gian Domenico Borasio, Ralf J Jox
At the end of life, several treatments are administered routinely that lack medical indication and may cause significant harm to patients. Examples include artificial hydration and oxygen therapy in the dying phase, as well as enteral nutrition in advanced dementia. Medical indication is defined as the appropriateness of a therapeutic or diagnostic measure in the patient's concrete clinical situation, in light of the best available evidence. The decision about the absence or presence of a medical indication is a core competence of physicians...
2016: Swiss Medical Weekly
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