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https://www.readbyqxmd.com/read/28722580/rapid-active-sampling-surveys-as-a-tool-to-evaluate-factors-associated-with-acute-gastroenteritis-and-norovirus-infection-among-children-in-rural-guatemala
#1
Daniel Olson, Molly M Lamb, Maria R Lopez, Maria A Paniagua-Avila, Alma Zacarias, Gabriela Samayoa-Reyes, Celia Cordon-Rosales, Edwin J Asturias
We examined burden and factors associated with norovirus (NoV) acute gastroenteritis (AGE) among children in rural Guatemala. Children age 6 weeks to 17 years were enrolled into three AGE surveillance groups, using two-stage cluster sampling: a prospective participatory syndromic surveillance (PSS) cohort and two cross-sectional rapid active sampling (RAS) surveys, conducted from April 2015 to February 2016. Epidemiologic and NoV testing data were used to identify factors associated with NoV infection, AGE, and NoV+ AGE...
June 19, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28722577/risk-factors-for-norovirus-gastroenteritis-among-nicaraguan-children
#2
Joann F Gruber, Natalie M Bowman, Sylvia Becker-Dreps, Yaoska Reyes, Connor Belson, Kenan C Michaels, Filemon Bucardo
Norovirus is a leading cause of pediatric gastroenteritis. Understanding norovirus epidemiology is essential for reducing disease burden. We conducted a case-control study to describe the distribution, clinical features, and risk factors of norovirus gastroenteritis among children < 5 years of age in León, Nicaragua. Cases were children testing positive for norovirus and controls were children living in the cases' communities. Study staff interviewed mothers of enrolled cases and controls to obtain detailed exposure information including food, water, and sanitation sources; recent exposures; household characteristics; and handwashing practices...
July 10, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28720280/hospitalised-rotavirus-gastroenteritis-in-new-zealand-the-laboratory-database-is-a-valuable-tool-for-assessing-the-impact-of-rotavirus-vaccination
#3
Matthew J Kelly, David Foley, Timothy K Blackmore
AIM: To assess the impact of the introduction of rotavirus vaccination in New Zealand at a regional and national level, underlining the utility of a passively collected laboratory dataset. METHOD: Retrospective laboratory data for rotavirus testing from Wellington and Hutt Hospitals from 1 January 2010 to 31 December 2016, matched with hospital admissions data of children under 5years of age with gastroenteritis primary and secondary coded admissions. The second part of the study examined the national dataset of primary coded hospital gastroenteritis admissions from the same period...
July 15, 2017: Vaccine
https://www.readbyqxmd.com/read/28719399/impact-of-rotavirus-vaccines-in-low-and-middle-income-countries
#4
Kulandaipalayam Natarajan Chella Sindhu, Sudhir Babji, Santhosh Kumar Ganesan
PURPOSE OF REVIEW: Rotavirus vaccines are playing a pivotal role in improving lives of infants and young children in low and middle-income countries (LMICs). Many of these countries have adopted the vaccine into their routine immunization, whereas others are considering introduction. This article provides an update on the impact of rotavirus vaccines in LMICs on morbidity and mortality in children aged less than 5 years, and their cost-effectiveness. RECENT FINDINGS: The WHO, in 2013, updated its recommendation to prioritize introduction of rotavirus vaccines in the routine immunization schedule, without age restrictions...
July 15, 2017: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/28718488/infectious-disease-morbidity-and-growth-among-young-hiv-exposed-uninfected-children-in-jamaica
#5
Russell B Pierre, Toni-Anne Fulford, Kaye Lewis, Paulette Palmer, Christine Walters, Celia D C Christie
Objective: There is a growing body of data that demonstrates increased infectious disease outcomes for HIV-exposed uninfected (HIV-EU) infants as compared to their HIV-unexposed (HU) counterparts. We hypothesized that these HIV-EU infants are at greater risk for infectious morbidity and mortality when compared to the general childhood population. We therefore aimed to characterize infections and growth outcomes among HIV-EU infants in Jamaica during their first two years of life. By identifying these outcomes, specific interventions could be implemented to mitigate this risk of morbidity and mortality...
December 2016: Revista Panamericana de Salud Pública, Pan American Journal of Public Health
https://www.readbyqxmd.com/read/28717848/new-identification-of-three-or-more-campylobacter-species-on-the-basis-of-a-degenerate-pcr-rflp-method-targeting-gyrb-gene
#6
Hiroyuki Fukuda, Fumiko Kojima, Shuji Fujimoto
This method was aimed targeting more Campylobacter species than conventional PCR-based identifications. They generally use species-specific primers focusing on clinically common species like C. jejuni, resulting in failure to recognize other species. We made the PCR-based identification more flexible using degenerate primers and DdeI- and MboI-separately used RFLP assay, which were designed on the basis of gyrB nucleotide sequence data of 14 Campylobacter species including C. jejuni, C. coli, and C. fetus. Ninety-four clinical isolates from patients with Campylobacter gastroenteritis and 13 biochemically identified C...
July 17, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28717574/detection-of-rotavirus-in-clinical-specimens-using-an-immunosensor-prototype-based-on-the-photon-burst-counting-technique
#7
Makoto Hasegawa, Ernest Apondi Wandera, Yuka Inoue, Nanami Kimura, Ryuzo Sasaki, Tamio Mizukami, Mohammad Monir Shah, Nobuaki Shirai, Osamu Takei, Hironori Shindo, Yoshio Ichinose
In this study, a sensitive fluorescence sensor was developed for the detection of small, fluorescence-labeled particles dispersed in a solution. The prototype system comprises of a laser confocal optical system and a mechanical sample stage to detect photon bursting of fluorescence-labeled small particles in sample volumes less than 5 μL within 3 minutes. To examine the feasibility of the prototype system as a diagnostic tool, assemblages of rotavirus and fluorescence-labeled antibody were analyzed. The detection sensitivity for rotavirus was 1 × 10(4) pfu/mL...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28717315/seroprevalence-of-antibodies-to-astrovirus-in-chickens-in-grenada-west-indies
#8
Ravindra Nath Sharma, Romane Dufayet, Thomas Maufras, Kathryn O' Connell, Keshaw Tiwari
AIM: Chicken astroviruses (CAstV) are known to cause mild gastroenteritis, growth depression, and even mortality in poultry, especially in chickens, turkeys, and ducks. To the best our knowledge, there is no published information on CAstV in Grenada. This study was conducted to determine the prevalence of astrovirus in chickens in Grenada. MATERIALS AND METHODS: Blood samples from 366 indigenous chickens and 92 commercial chicken layers were collected from all parishes of the island and tested for antibodies against CAstV using commercial enzyme-linked immunosorbent assay...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28716388/identification-of-co-infection-by-rotavirus-and-parvovirus-in-dogs-with-gastroenteritis-in-mexico
#9
Ariadna Flores Ortega, José Simón Martínez-Castañeda, Linda G Bautista-Gómez, Raúl Fajardo Muñoz, Israel Quijano Hernández
This is the first report on circulating canine rotavirus in Mexico. Fifty samples from dogs with gastroenteritis were analyzed used polymerase chain reaction and reverse transcription polymerase chain reaction in order to identify parvovirus and rotavirus, respectively; 7% of dogs were infected with rotavirus exclusively, while 14% were co-infected with both rotavirus and parvovirus; clinical signs in co-infected dogs were more severe.
June 24, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28716110/phylogenic-analysis-of-human-bocavirus-detected-in-children-with-acute-respiratory-infection-in-yaounde-cameroon
#10
Sebastien Kenmoe, Marie-Astrid Vernet, Mohamadou Njankouo-Ripa, Véronique Beng Penlap, Astrid Vabret, Richard Njouom
OBJECTIVE: Human Bocavirus (HBoV) was first identified in 2005 and has been shown to be a common cause of respiratory infections and gastroenteritis in children. In a recent study, we found that 10.7% of children with acute respiratory infections (ARI) were infected by HBoV. Genetic characterization of this virus remains unknown in Central Africa, particularly in Cameroon Leeding us to evaluate the molecular characteristics of HBoV strains in Cameroonian children with ARI. RESULTS: Phylogenetic analysis of partial HBoV VP1/2 sequences showed a low level of nucleotide variation and the circulation of HBoV genotype 1 (HBoV-1) only...
July 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28716067/molecular-characterization-of-group-a-rotaviruses-in-mukuru-slums-kenya-detection-of-novel-strains-circulating-in-children-below-5%C3%A2-years-of-age
#11
Joshua Ndung'u Gikonyo, James Nyangao, Cecilia Mbae, Carlene Sang, Eliud Njagi, Joseph Ngeranwa, Mathew Esona, Mapaseka L Seheri, Grace W Gitau, Kedra Raini, Samuel Kariuki
BACKGROUND: Gastroenteritis is a public health concern due to high morbidity and mortality among children. Rotaviruses are the leading etiological agents of severe gastroenteritis in children and accounts for more than half a million deaths per year in Africa. The study aimed at investigating the rotavirus genotypes that were circulating in children aged 5 years and below in and around Mukuru slums in Nairobi County Kenya. METHODS: A purposive cross sectional sampling method was applied where 166 samples were collected from children below 5 years of age and taken to Kenya Medical Research Institute virology laboratory...
July 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28714890/cardamom-cumin-and-dill-weed-essential-oils-chemical-compositions-antimicrobial-activities-and-mechanisms-of-action-against-campylobacter-spp
#12
Aysegul Mutlu-Ingok, Funda Karbancioglu-Guler
Natural antimicrobials as well as essential oils (EOs) have gained interest to inhibit pathogenic microorganisms and to control food borne diseases. Campylobacter spp. are one of the most common causative agents of gastroenteritis. In this study, cardamom, cumin, and dill weed EOs were evaluated for their antibacterial activities against Campylobacter jejuni and Campylobacter coli by using agar-well diffusion and broth microdilution methods, along with the mechanisms of antimicrobial action. Chemical compositions of EOs were also tested by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS)...
July 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28714223/relationship-between-uric-acid-and-dehydration-in-children-with-gastroenteritis
#13
Rie Kuge, Yoshihiko Morikawa, Yukihiro Hasegawa
BACKGROUND: Clinical assessment of dehydration in children with acute gastroenteritis (AGE) is crucial for treatment. The gold standard for assessment is acute weight loss. A previous study showed dehydration severity, as determined by weight loss, significantly correlated with serum urea (UN) in children with AGE. However, the limitation of using UN as a marker of dehydration severity is that the level can be affected by nutrition. Serum uric acid (UA) theoretically could serve as an alternative in this regard, but there have been no reports analyzing a direct relationship between serum UA and weight loss...
July 17, 2017: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/28712663/rectal-swabs-a-diagnostic-alternative-in-paediatric-gastroenteritis
#14
Eytan Wine
No abstract text is available yet for this article.
July 13, 2017: Lancet. Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28711627/vasoactive-intestinal-polypeptide-and-mast-cells-regulate-increased-passage-of-colonic-bacteria-in-patients-with-irritable-bowel-syndrome
#15
Olga Bednarska, Susanna A Walter, Maite Casado-Bedmar, Magnus Ström, Eloísa Salvo-Romero, Maria Vicario, Emeran A Mayer, Åsa V Keita
BACKGROUND & AIMS: Irritable bowel syndrome (IBS) is associated with intestinal dysbiosis and symptoms of IBS develop following gastroenteritis. We aimed to study passage of live bacteria through the colonic epithelium, and determine the role of mast cells and vasoactive intestinal polypeptide (VIP) in barrier regulation in IBS and healthy individuals. METHODS: Colon biopsies from 32 women with IBS and 15 age-matched healthy women (controls) were mounted in Ussing chambers; we measured numbers of fluorescently labeled Escherichia coli HS and Salmonella typhimurium that passed through from the mucosal side to the serosal side of the tissue...
July 12, 2017: Gastroenterology
https://www.readbyqxmd.com/read/28710858/identification-of-group-b-rotavirus-as-an-etiological-agent-in-the-gastroenteritis-outbreak-in-maharashtra-india
#16
M S Joshi, N N Ganorkar, S S Ranshing, A Basu, N A Chavan, V Gopalkrishna
Acute gastroenteritis outbreak occurred at Pargaon, Maharashtra, India in 1789 cases with an attack rate of 32.5% between November to December 2015. The stool specimens (n = 32) were investigated for different enteric viral agents using conventional methods. Transmission electron microscopy and RNA polyacrylamide gel electrophoresis respectively identified morphologically distinct rotavirus particles in 28% and RNA migration pattern of Group B Rotavirus (GBR) in 72% of the specimens. Reverse transcription polymerase chain reaction and nucleotide sequencing confirmed presence of GBR in 97% of the samples analyzed...
July 15, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28710688/death-by-food
#17
Roger W Byard
Although death from food is not an uncommon finding in forensic facilities worldwide, the range of underlying lethal mechanisms and associated conditions that should be sought at the time of autopsy is quite disparate. Deaths may occur from i) infectious agents including bacteria, viruses, protozoa, cestodes, nematodes and prions; ii) natural toxins including amanita toxins, tetrodotoxin, ciguatera and scombroid; iii) anaphylaxis; iv) poisoning; v) mechanical issues around airway and gut obstruction and/or perforation; and vi) miscellaneous causes...
July 15, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28707272/first-molecular-characterization-of-canine-parvovirus-strains-in-sardinia-italy
#18
S Dei Giudici, T Cubeddu, A Giagu, G Sanna, S Rocca, A Oggiano
Canine parvovirus type 2 (CPV-2) is responsible of acute hemorrhagic gastroenteritis in young dogs. CPV-2 emerged in 1978 in the USA, but new antigenic types, CPV-2a, 2b and 2c, have completely replaced the original type. In this study, we analyzed 81 animals collected in Sardinia, Italy. The VP2 sequence analysis of 27 positive samples showed that all antigenic CPV-2 types are circulating. CPV-2b seems to be the most widespread variant, followed by CPV-2a. Furthermore, 12 CPV-2b strains displayed further amino acid substitutions and formed a separate cluster in a phylogenetic tree, indicating regional genetic variation...
July 14, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28702797/purification-and-antibacterial-mechanism-of-fish-borne-bacteriocin-and-its-application-in-shrimp-penaeus-vannamei-for-inhibiting-vibrio-parahaemolyticus
#19
Xinran Lv, Jingfang Du, Yu Jie, Bolin Zhang, Fengling Bai, Hongfei Zhao, Jianrong Li
Vibrio parahaemolyticus: is recognized as the main cause of gastroenteritis associated with consumption of seafood. Bacteriocin-producing Lactobacillus plantarum FGC-12 isolated from golden carp intestine had strong antibacterial activity toward V. parahaemolyticus. The fish-borne bacteriocin was purified by a three-step procedure consisting of ethyl acetate extraction, gel filtration chromatography and high performance liquid chromatography. Its molecular weight was estimated at 4.1 kDa using SDS-PAGE. The fish-borne bacteriocin reached the maximum production at stationary phase after 20 h...
August 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28702101/rotavirus-genotypes-in-children-with-gastroenteritis-in-erzurum-first-detection-of-g12p-6-and-g12p-8-genotypes-in-turkey
#20
Hakan Aydin, Osman Aktaş
INTRODUCTION: Rotavirus is one of the leading pathogens which cause acute gastroenteritis in children and is responsible for a substantial proportion of childhood deaths worldwide. AIM: To determine the group A rotavirus (RVA) prevalence and genotypes of circulating RVA strains in 0-5-year-old children with complaints of vomiting and diarrhoea in Eastern Anatolia in Turkey. MATERIAL AND METHODS: RNA extracted from stool specimens of 329 children aged 0-5 years with acute diarrhoea was subjected to reverse transcription polymerase reaction (RT-PCR) and multiplex-nested PCR...
2017: Przegla̜d Gastroenterologiczny
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