Jiajia Zou, Ashley C Stammers, Andrea Taladriz-Sender, Jamie M Withers, Iain Christie, Marina Santana Vega, Badri L Aekbote, William J Peveler, David A Rusling, Glenn A Burley, Alasdair W Clark
An orthogonal, noncovalent approach to direct the assembly of higher-order DNA origami nanostructures is described. By incorporating perfluorinated tags into the edges of DNA origami tiles we control their hierarchical assembly via fluorous-directed recognition. When we combine this approach with Watson-Crick base-pairing we form discrete dimeric constructs in significantly higher yield (8x) than when either molecular recognition method is used in isolation. This integrated "catch-and-latch" approach, which combines the strength and mobility of the fluorous effect with the specificity of base-pairing, provides an additional toolset for DNA nanotechnology, one that enables increased assembly efficiency while requiring significantly fewer DNA sequences...
January 10, 2023: ACS Nano