Min Ji Cho, Dong Gwang Lee, Jeong Woong Lee, Byungtae Hwang, Sung-Jin Yoon, Seon-Jin Lee, Young-Jun Park, Seung-Ho Park, Hee Gu Lee, Yong-Hoon Kim, Chul-Ho Lee, Jangwook Lee, Nam-Kyung Lee, Tae-Su Han, Hyun-Soo Cho, Jeong Hee Moon, Ga Seul Lee, Kwang-Hee Bae, Geum-Sook Hwang, Sang-Hak Lee, Sang J Chung, Sungbo Shim, Jaehyung Cho, Goo Taeg Oh, Young-Guen Kwon, Jong-Gil Park, Jeong-Ki Min
AIMS: The nuclear factor-κB (NF-κB) signalling pathway plays a critical role in the pathogenesis of multiple vascular diseases. However, in endothelial cells (ECs), the molecular mechanisms responsible for the negative regulation of the NF-κB pathway are poorly understood. In this study, we investigated a novel role for protein tyrosine phosphatase type IVA1 (PTP4A1) in NF-κB signalling in ECs. METHODS AND RESULTS: In human tissues, human umbilical artery ECs, and mouse models for loss of function and gain of function of PTP4A1, we conducted histological analysis, immunostaining, laser-captured microdissection assay, lentiviral infection, small interfering RNA transfection, quantitative real-time PCR and reverse transcription-PCR, as well as luciferase reporter gene and chromatin immunoprecipitation assays...
December 19, 2022: Cardiovascular Research