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https://www.readbyqxmd.com/read/27693430/mir-483-is-a-self-regulating-microrna-and-can-activate-its-own-expression-via-usf1-in-hela-cells
#1
Verena Vanessa Emmerling, Simon Fischer, Michael Kleemann, René Handrick, Stefan Kochanek, Kerstin Otte
MicroRNAs (miRNAs) are small non-coding RNAs that constitute a fundamental part of post-transcriptional gene regulation in mammalian cells. We have recently identified the intronic miR-483, which functions as an important regulator of protein synthesis during mild hypothermia in human and rodent cells. Since only very little is known about transcriptional regulation of intronic miRNAs and their host genes, we thoroughly investigated the regulation of miR-483 expression and its host gene IGF2 in HeLa cells. We demonstrate that miR-483 is regulated and expressed independently of its host gene IGF2 during mild hypothermia...
September 28, 2016: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/27649659/usf1-prompt-melanoma-through-upregulating-tgf-%C3%AE-signaling-pathway
#2
Y-Q Ren, Q-H Li, L-B Liu
OBJECTIVE: The activation of TGF-β signaling contributes to abnormal EMT process and upstream stimulatory family1 (USF1) was recently found to activate the expression of TGF-β. However, the specific role of USF1 in melanoma has never been explored. MATERIALS AND METHODS: The expression of USF1 was analyzed using real-time PCR and Western blot. The changes of cell morphology were observed under a microscope. Cell migration was determined using in vitro scratch test...
September 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27485769/5-hydroxymethylcytosine-in-e-box-motifs-acat-gtg-and-acac-gtg-increases-dna-binding-of-the-b-hlh-transcription-factor-tcf4
#3
Syed Khund-Sayeed, Ximiao He, Timothy Holzberg, Jun Wang, Divya Rajagopal, Shriyash Upadhyay, Stewart R Durell, Sanjit Mukherjee, Matthew T Weirauch, Robert Rose, Charles Vinson
We evaluated DNA binding of the B-HLH family members TCF4 and USF1 using protein binding microarrays (PBMs) containing double-stranded DNA probes with cytosine on both strands or 5-methylcytosine (5mC) or 5-hydroxymethylcytosine (5hmC) on one DNA strand and cytosine on the second strand. TCF4 preferentially bound the E-box motif (CAN|NTG) with strongest binding to the 8-mer CAG|GTGGT. 5mC uniformly decreases DNA binding of both TCF4 and USF1. The bulkier 5hmC also inhibited USF1 binding to DNA. In contrast, 5hmC dramatically enhanced TCF4 binding to E-box motifs ACAT|GTG and ACAC|GTG, being better bound than any 8-mer containing cytosine...
September 12, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/27439518/c-src-suppresses-dendritic-cell-antitumor-activity-via-t-cell-ig-and-mucin-protein-3-receptor
#4
Ravindra Gujar, Neeraj Maurya, Vinod Yadav, Mamta Gupta, Saurabh Arora, Neeraj Khatri, Pradip Sen
The enhanced expression of T cell Ig and mucin protein-3 (TIM-3) on tumor-associated dendritic cells (DCs) attenuates antitumor effects of DNA vaccines. To identify a potential target (or targets) for reducing TIM-3 expression on tumor-associated DCs, we explored the molecular mechanisms regulating TIM-3 expression. In this study, we have identified a novel signaling pathway (c-Src→Bruton's tyrosine kinase→transcription factors Ets1, Ets2, USF1, and USF2) necessary for TIM-3 upregulation on DCs. Both IL-10 and TGF-β, which are produced in the tumor microenvironment, upregulated TIM-3 expression on DCs via this pathway...
September 1, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27141965/setd1a-and-nurf-mediate-chromatin-dynamics-and-gene-regulation-during-erythroid-lineage-commitment-and-differentiation
#5
Ying Li, Vincent P Schulz, Changwang Deng, Guangyao Li, Yong Shen, Betsabeh K Tusi, Gina Ma, Jared Stees, Yi Qiu, Laurie A Steiner, Lei Zhou, Keji Zhao, Jörg Bungert, Patrick G Gallagher, Suming Huang
The modulation of chromatin structure is a key step in transcription regulation in mammalian cells and eventually determines lineage commitment and differentiation. USF1/2, Setd1a and NURF complexes interact to regulate chromatin architecture in erythropoiesis, but the mechanistic basis for this regulation is hitherto unknown. Here we showed that Setd1a and NURF complexes bind to promoters to control chromatin structural alterations and gene activation in a cell context dependent manner. In human primary erythroid cells USF1/2, H3K4me3 and the NURF complex were significantly co-enriched at transcription start sites of erythroid genes, and their binding was associated with promoter/enhancer accessibility that resulted from nucleosome repositioning...
September 6, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/26920453/the-pregnane-x-receptor-down-regulates-organic-cation-transporter-1-slc22a1-in-human-hepatocytes-by-competing-for-squelching-src-1-coactivator
#6
Lucie Hyrsova, Tomas Smutny, Alejandro Carazo, Stefan Moravcik, Jana Mandikova, Frantisek Trejtnar, Sabine Gerbal-Chaloin, Petr Pavek
BACKGROUND AND PURPOSE: The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters in the liver. Here, we have assessed the down-regulation of OCT1 expression by PXR activation. EXPERIMENTAL APPROACH: We used primary human hepatocytes and related cell lines to measure OCT1 expression and activity, by assaying MPP(+) accumulation...
May 2016: British Journal of Pharmacology
https://www.readbyqxmd.com/read/26819196/usf1-deficiency-activates-brown-adipose-tissue-and-improves-cardiometabolic-health
#7
Pirkka-Pekka Laurila, Jarkko Soronen, Sander Kooijman, Saara Forsström, Mariëtte R Boon, Ida Surakka, Essi Kaiharju, Claudia P Coomans, Sjoerd A A Van Den Berg, Anu Autio, Antti-Pekka Sarin, Johannes Kettunen, Emmi Tikkanen, Tuula Manninen, Jari Metso, Reija Silvennoinen, Krista Merikanto, Maija Ruuth, Julia Perttilä, Anne Mäkelä, Ayaka Isomi, Anita M Tuomainen, Anna Tikka, Usama Abo Ramadan, Ilkka Seppälä, Terho Lehtimäki, Johan Eriksson, Aki Havulinna, Antti Jula, Pekka J Karhunen, Veikko Salomaa, Markus Perola, Christian Ehnholm, Miriam Lee-Rueckert, Miranda Van Eck, Anne Roivainen, Marja-Riitta Taskinen, Leena Peltonen, Eero Mervaala, Anu Jalanko, Esa Hohtola, Vesa M Olkkonen, Samuli Ripatti, Petri T Kovanen, Patrick C N Rensen, Anu Suomalainen, Matti Jauhiainen
USF1 (upstream stimulatory factor 1) is a transcription factor associated with familial combined hyperlipidemia and coronary artery disease in humans. However, whether USF1 is beneficial or detrimental to cardiometabolic health has not been addressed. By inactivating USF1 in mice, we demonstrate protection against diet-induced dyslipidemia, obesity, insulin resistance, hepatic steatosis, and atherosclerosis. The favorable plasma lipid profile, including increased high-density lipoprotein cholesterol and decreased triglycerides, was coupled with increased energy expenditure due to activation of brown adipose tissue (BAT)...
January 27, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/26772882/basal-transcription-of-apobec3g-is-regulated-by-usf1-gene-in-hepatocyte
#8
Yanli Zeng, Hui Li, Xiaoju Zhang, Jia Shang, Yi Kang
Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3G (APOBEC3G, A3G) exert antiviral defense as an important factor of innate immunity. A variety of cytokines such as IFN-γ、IL2、IL15、IL7 could induce the transcription of A3G. However, the regulation of other nuclear factor on the transcription of A3G have not been reported at the present. To gain new insights into the transcriptional regulation of this restriction factor, we cloned and characterized the promoter region of A3G and investigate the modulation of USF1 gene on the transcription of A3G...
January 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26756308/genome-wide-analyses-in-neuronal-cells-reveal-that-upstream-transcription-factors-regulate-lysosomal-gene-expression
#9
Tomoyuki Yamanaka, Asako Tosaki, Masaru Kurosawa, Tomomi Shimogori, Nobutaka Hattori, Nobuyuki Nukina
UNLABELLED: The upstream transcription factors (USFs) USF1 and USF2 are ubiquitously expressed transcription factors that are characterized by a conserved basic helix-loop-helix/leucine zipper DNA-binding domain. They form homo- or heterodimers, and recognize E-box motifs to modulate gene expression. They are known to regulate diverse cellular functions, including the cell cycle, immune responses and glucose/lipid metabolism, but their roles in neuronal cells remain to be clarified. Here, we performed chromatin immunoprecipitation of USF1 from mouse brain cortex...
March 2016: FEBS Journal
https://www.readbyqxmd.com/read/26629988/bioinformatics-analysis-of-the-effects-of-tobacco-smoke-on-gene-expression
#10
Chunhua Cao, Jianhua Chen, Chengqi Lyu, Jia Yu, Wei Zhao, Yi Wang, Derong Zou
This study was designed to explore the effects of tobacco smoke on gene expression through bioinformatics analyses. Gene expression profile GSE17913 was downloaded from the Gene Expression Omnibus database. The differentially expressed genes (DEGs) in buccal mucosa tissues between 39 active smokers and 40 never smokers were identified. Gene Ontology Specifically, the DEG distribution in the pathway of Metabolism of xenobiotics by cytochrome P450 was shown in Fig 2[corrected] were performed, followed by protein-protein interaction (PPI) network, transcriptional regulatory network as well as miRNA-target regulatory network construction...
2015: PloS One
https://www.readbyqxmd.com/read/26577526/the-nuclear-fraction-of-protein-kinase-ck2-binds-to-the-upstream-stimulatory-factors-usfs-in-the-absence-of-dna
#11
Sarah Spohrer, Elitsa Y Dimova, Thomas Kietzmann, Mathias Montenarh, Claudia Götz
The functions of the upstream stimulatory factors USF1 and USF2 are, like those of other transcription factors, regulated by reversible phosphorylation. Besides many other kinases also protein kinase CK2 phosphorylates USF1 but not USF2. In a yeast-two-hybrid screen, however, the non-catalytic CK2β subunit of CK2 was identified as a binding partner of USF2. This surprising observation prompted us to investigate the CK2/USF interaction in more detail in the present study. By using immunofluorescence analyses as well as co-immunoprecipitations we found that USF1 and USF2 bound to CK2α and CK2β exclusively in the nucleus, though CK2β and to a minor amount CK2α were also present in the cytoplasm...
February 2016: Cellular Signalling
https://www.readbyqxmd.com/read/26535571/-e-4-3-4-dimethoxyphenyl-but-3-en-1-ol-enhances-melanogenesis-through-increasing-upstream-stimulating-factor-1-mediated-tyrosinase-expression
#12
Jisu Park, Heesung Chung, Seung Hyun Bang, Ah-Reum Han, Eun-Kyoung Seo, Sung Eun Chang, Duk-Hee Kang, Eok-Soo Oh
We investigated the potential melanogenic effect of compounds from Zingiber cassumunar Roxb. Our data revealed that chloroform-soluble extract of Z. cassumunar enhanced melanin synthesis in B16F10 melanoma cells. Among the components of the chloroform extract, (E)-4-(3,4-dimethoxyphenyl)but-3-en-1-ol (DMPB) increased melanogenesis in both B16F10 cells and human primary melanocytes. In B16F10 cells, DMPB enhanced the activation of ERK and p38, and the level of tyrosinase. Although the level of microphthalmia-associated transcription factor was unchanged in DMPB-treated B16F10 cells, DMPB increased levels and nuclear localization of upstream stimulating factor-1 (USF1)...
2015: PloS One
https://www.readbyqxmd.com/read/26527095/dissecting-daily-and-circadian-expression-rhythms-of-clock-controlled-genes-in-human-blood
#13
Karolina Lech, Katrin Ackermann, Victoria L Revell, Oscar Lao, Debra J Skene, Manfred Kayser
The identification and investigation of novel clock-controlled genes (CCGs) has been conducted thus far mainly in model organisms such as nocturnal rodents, with limited information in humans. Here, we aimed to characterize daily and circadian expression rhythms of CCGs in human peripheral blood during a sleep/sleep deprivation (S/SD) study and a constant routine (CR) study. Blood expression levels of 9 candidate CCGs (SREBF1, TRIB1, USF1, THRA1, SIRT1, STAT3, CAPRIN1, MKNK2, and ROCK2), were measured across 48 h in 12 participants in the S/SD study and across 33 h in 12 participants in the CR study...
February 2016: Journal of Biological Rhythms
https://www.readbyqxmd.com/read/26297580/transcriptional-activation-of-epstein-barr-virus-brlf1-by-usf1-and-rta
#14
Chen-Chia Hung, Chung-Wen Kuo, Wen-Hung Wang, Tzu-Hsuan Chang, Pey-Jium Chang, Li-Kwan Chang, Shih-Tung Liu
During its lytic cycle, Epstein-Barr virus (EBV) expresses Rta, a factor encoded by BRLF1 that activates the transcription of viral lytic genes. We found that upstream stimulating factor (USF) binds to E1, one of the five E boxes located at - 79 in the BRLF1 promoter (Rp), to activate BRLF1 transcription. Furthermore, Rta was shown to interact with USF1 in coimmunoprecipitation and glutathione S-transferase (GST)-pulldown assays, and confocal laser-scanning microscopy further confirmed that these two proteins colocalize in the nucleus...
September 2015: Journal of General Virology
https://www.readbyqxmd.com/read/26102644/1a-03-transcriptional-network-associated-with-the-contractile-phenotype-of-smooth-muscle-cells-in-human-carotid-atherosclerosis
#15
C Cerutti, C Z Paultre, M P Gustin, O Lohez, P Feugier, J Y Li, G Bricca
OBJECTIVE: During atherogenesis, vascular smooth muscle cells (VSMCs) undergo a phenotypic modulation leading to migration and loss of contractility. Here we propose a gene regulatory network specific of the contractile phenotype of the carotid VSMCs from transcriptomic data. DESIGN AND METHOD: Human carotid atheroma plaque (ATH, Stary>4) and nearby macroscopically intact tissue (MIT, Stary<3) of 32 patients were analysed by microarrays (Affymetrix HuGene-1...
June 2015: Journal of Hypertension
https://www.readbyqxmd.com/read/26068452/the-rs2516839-polymorphism-of-the-usf1-gene-may-modulate-serum-triglyceride-levels-in-response-to-cigarette-smoking
#16
Pawel Niemiec, Tomasz Nowak, Tomasz Iwanicki, Sylwia Gorczynska-Kosiorz, Anna Balcerzyk, Jolanta Krauze, Wladyslaw Grzeszczak, Maria Wiecha, Iwona Zak
Single nucleotide polymorphisms (SNPs) of the USF1 gene (upstream stimulatory factor 1) influence plasma lipid levels. This study aims to determine whether USF1 SNPs interact with traditional risk factors of atherosclerosis to increase coronary artery disease (CAD) risk. In the present study serum lipid levels and USF1 gene polymorphisms (rs2516839 and rs3737787) were determined in 470 subjects: 235 patients with premature CAD and 235 controls. A trend of increasing triglycerides (TG) levels in relation to the C allele dose of rs2516839 SNP was observed...
2015: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/26052935/association-between-single-nucleotide-polymorphisms-of-upstream-transcription-factor-1-usf1-and-susceptibility-to-papillary-thyroid-cancer
#17
Qingzhong Yuan, Qingao Bu, Guoqiang Li, Jun Zhang, Tao Cui, Rui Zhu, Dongpo Mu
BACKGROUND: Thyroid cancer, predominantly by papillary thyroid cancer (PTC), is a malignant tumour of endocrine system with increasing incidence rate worldwide. Upstream transcription factor 1 (USF1) regulates a variety of biological processes by transactivation of functional genes. In this study, we investigated the association between USF1 polymorphisms and PTC risk. MATERIAL & METHODS: A total of 334 patients with PTC, 186 patients with benign nodules (BN) and 668 healthy controls were enrolled in our study...
April 2016: Clinical Endocrinology
https://www.readbyqxmd.com/read/26034040/vascular-smooth-muscle-lrp6-limits-arteriosclerotic-calcification-in-diabetic-ldlr-mice-by-restraining-noncanonical-wnt-signals
#18
Su-Li Cheng, Bindu Ramachandran, Abraham Behrmann, Jian-Su Shao, Megan Mead, Carolyn Smith, Karen Krchma, Yoanna Bello Arredondo, Attila Kovacs, Kapil Kapoor, Laurence M Brill, Ranjan Perera, Bart O Williams, Dwight A Towler
RATIONALE: Wnt signaling regulates key aspects of diabetic vascular disease. OBJECTIVE: We generated SM22-Cre;LRP6(fl/fl);LDLR(-/-) mice to determine contributions of Wnt coreceptor low-density lipoprotein receptor-related protein 6 (LRP6) in the vascular smooth muscle lineage of male low-density lipoprotein receptor-null mice, a background susceptible to diet (high-fat diet)-induced diabetic arteriosclerosis. METHODS AND RESULTS: As compared with LRP6(fl/fl);LDLR(-/-) controls, SM22-Cre;LRP6(fl/fl);LDLR(-/-) (LRP6-VKO) siblings exhibited increased aortic calcification on high-fat diet without changes in fasting glucose, lipids, or body composition...
July 3, 2015: Circulation Research
https://www.readbyqxmd.com/read/25932200/usf-1-genetic-polymorphisms-confer-a-high-risk-of-nonalcoholic-fatty-liver-disease-in-chinese-population
#19
Ying Wang, Bai-Fang Wang, Jing Tong, Bing Chang, Bing-Yuan Wang
Genetic polymorphisms in upstream transcription factor 1 (USF1) were investigated for their links to increased risk of nonalcoholic fatty liver disease (NAFLD) in Chinese population. Between January 2013 and April 2014, 174 patients with NAFLD in the First Affiliated Hospital of China Medical University were selected for this study. A group of 100 healthy subjects were identified as the control group. The MALDI-TOF-MS, a mass spectrometry based technique, was used to detect USF-1 genetic polymorphisms using PCR amplified DNA products...
2015: International Journal of Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/25741280/protein-kinases-as-switches-for-the-function-of-upstream-stimulatory-factors-implications-for-tissue-injury-and-cancer
#20
Tina Horbach, Claudia Götz, Thomas Kietzmann, Elitsa Y Dimova
The upstream stimulatory factors (USFs) are regulators of important cellular processes. Both USF1 and USF2 are supposed to have major roles in metabolism, tissue protection and tumor development. However, the knowledge about the mechanisms that control the function of USFs, in particular in tissue protection and cancer, is limited. Phosphorylation is a versatile tool to regulate protein functions. Thereby, phosphorylation can positively or negatively affect different aspects of transcription factor function including protein stability, protein-protein interaction, cellular localization, or DNA binding...
2015: Frontiers in Pharmacology
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