Jiawei Shao, Shichao Li, Xinyuan Qiu, Jian Jiang, Lihang Zhang, Pengli Wang, Yaqing Si, Yuhang Wu, Minghui He, Qiqi Xiong, Liuqi Zhao, Yilin Li, Yuxuan Fan, Mirta Viviani, Yu Fu, Chaohua Wu, Ting Gao, Lingyun Zhu, Martin Fussenegger, Hui Wang, Mingqi Xie
Here, we present a gene regulation strategy enabling programmable control over eukaryotic translational initiation. By excising the natural poly-adenylation (poly-A) signal of target genes and replacing it with a synthetic control region harboring RNA-binding protein (RBP)-specific aptamers, cap-dependent translation is rendered exclusively dependent on synthetic translation initiation factors (STIFs) containing different RBPs engineered to conditionally associate with different eIF4F-binding proteins (eIFBPs)...
January 4, 2024: Cell Research