Marcin Warminski, Edyta Trepkowska, Miroslaw Smietanski, Pawel J Sikorski, Marek R Baranowski, Marcelina Bednarczyk, Hanna Kedzierska, Bartosz Majewski, Adam Mamot, Diana Papiernik, Agnieszka Popielec, Remigiusz A Serwa, Brittany A Shimanski, Piotr Sklepkiewicz, Marta Sklucka, Olga Sokolowska, Tomasz Spiewla, Diana Toczydlowska-Socha, Zofia Warminska, Karol Wolosewicz, Joanna Zuberek, Jeffrey S Mugridge, Dominika Nowis, Jakub Golab, Jacek Jemielity, Joanna Kowalska
Eukaryotic mRNAs undergo cotranscriptional 5'-end modification with a 7-methylguanosine cap. In higher eukaryotes, the cap carries additional methylations, such as m6 Am ─a common epitranscriptomic mark unique to the mRNA 5'-end. This modification is regulated by the Pcif1 methyltransferase and the FTO demethylase, but its biological function is still unknown. Here, we designed and synthesized a trinucleotide FTO-resistant N 6-benzyl analogue of the m6 Am -cap-m7 GpppBn6 Am pG (termed AvantCap ) and incorporated it into mRNA using T7 polymerase...
March 5, 2024: Journal of the American Chemical Society