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https://www.readbyqxmd.com/read/28186672/bladder-transplantation-of-amniotic-fluid-stem-cell-may-ameliorate-bladder-dysfunction-after-focal-cerebral-ischemia-in-rat
#1
Ching-Chung Liang, S W Steven Shaw, Yung-Hsin Huang, Yi-Hao Lin, Tsong-Hai Lee
The objective is to investigate whether human amniotic fluid stem cells (hAFSCs) grafting into the bladder may influence bladder functional and molecular changes in an animal stroke model. Female rats were divided into three groups: sham, middle cerebral artery occlusion (MCAO) alone, and MCAO plus 1 × 10(6) hAFSCs transplanting into bladder wall. Bladder function was analyzed by cystometry at days 3 and 10 after MCAO. The expressions of bladder nerve growth factor (NGF), M2-muscarinic, M3-muscarinic, and P2X1 receptors were measured by immunohistochemistry and real-time polymerase chain reaction...
January 3, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28181685/effects-of-estrogen-receptor-%C3%AE-stimulation-in-a-rat-model-of-non-bacterial-prostatic-inflammation
#2
Shinsuke Mizoguchi, Kenichi Mori, Zhou Wang, Teresa Liu, Yasuhito Funahashi, Fuminori Sato, Donald B DeFranco, Naoki Yoshimura, Hiromitsu Mimata
BACKGROUND: There is increasing evidence showing that chronic non-bacterial prostatic inflammation is involved in the pathogenesis of benign prostatic hyperplasia (BPH) and male lower urinary tract symptoms (LUTS). It has also been reported that estrogen receptor β (ERβ) could have an immunoprotective role in prostatic tissue. Therefore, we investigated the effect of ERβ-activation on not only prostatic inflammation, but also bladder overactive conditions in a rat model with nonbacterial prostatic inflammation...
February 9, 2017: Prostate
https://www.readbyqxmd.com/read/28176494/ultrasound-bladder-wall-thickness-and-detrusor-overactivity-a-multicentre-test-accuracy-study
#3
P Latthe, L Middleton, S Rachaneni, S McCooty, J Daniels, A Coomarasamy, M Balogun, J Duckett, R Thakar, I Goranitis, T Roberts, J Deeks
OBJECTIVE: Women with overactive bladder (OAB) often undergo urodynamics before invasive treatments are considered. Ultrasound measurement of bladder wall thickness (BWT) is a less invasive, less expensive and widely available test. It has the potential to diagnose the presence of detrusor overactivity (DO). We aimed to evaluate the accuracy of BWT in the diagnosis of DO. DESIGN: Prospective cohort study. SETTING: Twenty-two UK clinics (university and district general hospitals)...
February 7, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28169451/an-optimized-transurethral-catheterization-cystometry-in-mice-and-comparison-with-classic-suprapubic-catheterization-cystometry
#4
Zhuoyi Xiang, Yaohui Li, Tingchang Bian, Minke He, Yeqing Xu, Guomin Wang, Jianming Guo, Hang Wang
AIMS: The aim of this study is to establish an optimized, minimally invasive transurethral catheterization cystometry (TUCC) and a novel urethral pressure profile (UPP) measurement for mice. METHODS: The optimized TUCC and the UPP measurement were first established. This optimized TUCC was then performed in 16 anesthetized female mice and compared with the suprapubic catheterization cystometry (SCC) in parallel after suprapubic catheters implantation (SCI; on zero, third, and seventh day, respectively)...
February 7, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28124533/cystometric-analysis-of-the-transplanted-bladder
#5
Jeová Nina Rocha
OBJECTIVE: cystometric evaluation of the bladder after autotransplant and isogeneic transplant in female rats. MATERIAL AND METHODS: two groups were constituted: (A) bladder autotransplant with two subgroups: R1 - (control) and R2 - (bladder transplant); (B) isogeneic bladder transplant with three subgroups; T1 - (control); T2-T3, two subgroups observed for 30 and 60 days after transplant, respectively. All animals underwent cystometric evaluation. Afterwards, the bladders were removed for histological study...
January 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28120449/correlation-between-advanced-glycation-end-products-lower-urinary-tract-symptoms-and-bladder-dysfunctions-in-patients-with-type-2-diabetes-mellitus
#6
Alessandro Galì, Giuseppe Mucciardi, Salvatore Butticè, Enrica Subba, Carmela D'Amico, Francesco Lembo, Carlo Magno
OBJECTIVES: To explore whether serum and urinary advanced glycation end-products (AGEs) are related to urinary symptoms and bladder dysfunctions in diabetic patients. METHODS: Forty-seven patients with type 2 Diabetes mellitus (T2DM) and lower urinary tract symptoms (LUTS) were enrolled. LUTS evaluation was performed by IPSS (International Prostatic Symptoms Score), QoL (quality of life), OAB (overactive bladder). ICI-SF (International Consultation on Incontinence - short form) quaestionneires; ultrasound examination, evaluation of postvoid residual (PVR), uroflowmetry, cystometry with pressure-flow study (PFS) were performed to detect bladder dysfunctions...
January 2017: Lower Urinary Tract Symptoms
https://www.readbyqxmd.com/read/28120447/effects-of-sildenafil-a-phosphodiesterase-type-5-inhibitor-on-the-primary-single-afferent-activity-of-the-rat-bladder
#7
Naoki Aizawa, Hiroki Ito, Rino Sugiyama, Tetsuya Fujimura, Hiroshi Fukuhara, Haruki Kume, Yukio Homma, Yasuhiko Igawa
OBJECTIVES: We investigated the direct effects of sildenafil, a phosphodiesterase type 5 inhibitor, on the single-unit mechanosensitive afferent activities (SAAs) primarily originated from the bladder in the rat. METHODS: Female Sprague-Dawley rats were anesthetized with urethane. SAAs were recorded from the left L6 dorsal roots and classified by conduction velocity as Aδ- or C-fibers. A catheter was inserted into the bladder dome, and a separate catheter was placed in the carotid artery and external iliac vein for monitoring of blood pressure and sildenafil-administration, respectively...
January 2017: Lower Urinary Tract Symptoms
https://www.readbyqxmd.com/read/28106785/ketamine-analog-methoxetamine-induced-inflammation-and-dysfunction-of-bladder-in-rats
#8
Qiang Wang, Qinghui Wu, Junpeng Wang, Yang Chen, Guihao Zhang, Jiawei Chen, Jie Zhao, Peng Wu
The novel synthetic psychoactive ketamine analog methoxetamine is reportedly being used for recreational purposes. As ketamine use can result in urinary dysfunction, we conducted the present study to investigate how methoxetamine affects the bladder. A cystometry investigation showed that female Sprague-Dawley rats experienced increased micturition frequency bladder dysfunction after receiving a daily intraperitoneal injection of 30 mg/kg methoxetamine or ketamine for periods of 4 or 12 weeks. Histologic examinations of rat bladder tissue revealed damaged urothelium barriers, as well as evidence of inflammatory cell infiltration and matrix deposition...
January 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28097266/the-effect-of-posture-and-repetition-on-urodynamic-parameters-a-prospective-randomized-study
#9
Ho Joon Jeon, Min Soo Choo, Seung-June Oh
PURPOSE: To evaluate the effect of posture and repetition of filling cystometry on urodynamic parameters. MATERIALS AND METHODS: Seventy-one men with benign prostatic hyperplasia participated in a urodynamic study between September 2015 and August 2016 and were randomly assigned to a supine to supine (group SS, n=16), erect to erect (group EE, n=16), supine to erect (group SE, n=19) or erect to supine (group ES, n=20) group. The patients underwent filling cystometry twice...
January 2017: Investigative and Clinical Urology
https://www.readbyqxmd.com/read/28081479/5-year-experience-in-the-diagnosis-and-treatment-of-occult-urinary-incontinence-in-women-with-pelvic-organ-prolapse
#10
Burcin Karamustafaoglu Balci, Funda Gungor Ugurlucan, Cenk Yasa, Onay Yalcin
OBJECTIVE: Our aim was to analyze our five-year experience in the diagnosis and treatment of occult urinary incontinence in women with pelvic organ prolapse. STUDY DESIGN: The medical records of all patients who were admitted to the Division of Urogynecology of Istanbul Faculty of Medicine between January 2008 and December 2013; in total, 1600 patients were retrospectively evaluated. The study population included 287 patients who had prolapse beyond the hymen and underwent two consecutive urodynamic studies with and without prolapse reduction...
January 5, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28060912/early-fesoterodine-fumarate-administration-prevents-neurogenic-detrusor-overactivity-in-a-spinal-cord-transected-rat-model
#11
Xavier Biardeau, Mikolaj Przydacz, Shachar Aharony, George Loutochin, Lysanne Campeau, Maeva Kyheng, Jacques Corcos
BACKGROUND: In spinal cord injury, onset of detrusor overactivity (DO) is detrimental for quality of life (incontinence) and renal risk. Prevention has only been achieved with complex sophisticated electrical neuromodulation techniques. PURPOSE: To assess the efficacy of early fesoterodine fumarate (FF) administration in preventing bladder overactivity in a spinal cord transected (SCT) rat model. METHODS: 33 Sprague-Dawley rats were allocated to 6 groups-Group 1: 3 normal controls; Group 2: 6 SCT controls; Group 3: 6 SCT rats + FF 0...
2017: PloS One
https://www.readbyqxmd.com/read/28052872/fgfr2-is-integral-for-bladder-mesenchyme-patterning-and-function
#12
Youko Ikeda, Irina Zabbarova, Caitlin M Schaefer, Daniel S Bushnell, William C de Groat, Anthony J Kanai, Carlton M Bates
BACKGROUND: While urothelial signals, including sonic hedgehog (Shh), drive bladder mesenchyme differentiation, it is unclear which pathways within the mesenchyme are critical for its development. Studies have shown fibroblast growth factor receptor 2 (Fgfr2) is necessary for kidney and ureter mesenchymal development. OBJECTIVE: Out objective was to determine the role of Fgfr2 in bladder mesenchyme. METHODS: We used Tbx18cre mice to delete Fgfr2 in bladder mesenchyme (Fgfr2BM-/-)...
January 4, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28035695/effects-of-low-energy-shock-wave-therapy-on-inflammatory-moleculars-bladder-pain-and-bladder-function-in-a-rat-cystitis-model
#13
Hung-Jen Wang, Wei-Chia Lee, Pradeep Tyagi, Chao-Cheng Huang, Yao-Chi Chuang
AIMS: Low energy shock wave (LESW) is known to facilitate tissue regeneration with analgesic and anti-inflammatory effects. We examined the effects of LESW on the expression of inflammatory molecules, pain behavior, and bladder function in a rat cystitis model. METHODS: Control and experimental animals were injected with saline or cyclophosphamide (CYP; 75 mg/kg intraperitoneally) on day 1 and 4. After lower midline incision, the bladders were exposed to LESW (300 pulses, 0...
December 30, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27999934/detrusor-pressures-in-urodynamic-studies-during-voiding-in-women
#14
Satoru Kira, Takahiko Mitsui, Hideki Kobayashi, Yaburu Haneda, Norifumi Sawada, Masayuki Takeda
INTRODUCTION AND HYPOTHESIS: To investigate detrusor pressure during voiding in women using urodynamic studies (UDS). METHODS: The study group comprised 57 women with non-neurogenic lower urinary tract symptoms. All patients underwent UDS between January 2010 and December 2014. UDS included filling cystometry, pressure flow study (PFS), uroflowmetry for the maximum flow rate (Qmax) and mean flow rate, and postvoid residuals. Existence of voluntary detrusor contraction was defined as a continuous and smooth increase in detrusor pressure (Pdet) after the instruction to micturate in the PFS...
December 20, 2016: International Urogynecology Journal
https://www.readbyqxmd.com/read/27995942/improvement-in-detrusor-sphincter-dyssynergia-by-bladder-wall-injection-of-replication-defective-herpes-simplex-virus-vector-mediated-gene-delivery-of-kynurenine-aminotransferase-ii-in-spinal-cord-injury-rats
#15
Z Wang, L Liao
STUDY DESIGN: An experimental study. OBJECTIVES: To investigate the effect of replication-defective herpes simplex virus (HSV) vectors encoding the kynurenine aminotransferase II (HSVrd-KATII) gene on detrusor-sphincter dyssynergia (DSD) in spinal cord injury (SCI) rats. SETTING: Beijing, China. METHODS: Sprague-Dawley rats (240-265 g) were spinalized with complete transaction at the T10 level of the spinal cord. The rats were randomly divided into the following three groups: sham group (n=12, with normal saline); HSVrd group (n=12, with HSVrd) and HSVrd-KATII group (n=12, with HSVrd-KATII)...
February 2017: Spinal Cord
https://www.readbyqxmd.com/read/27992393/manipulating-the-extracellular-matrix-an-animal-model-of-the-bladder-pain-syndrome
#16
Ifeoma Offiah, Athanasios Didangelos, Barry A OʼReilly, Stephen B McMahon
Bladder pain syndrome (BPS) is associated with breakdown of the protective uroepithelial barrier of the urinary bladder allowing urinary constituents access to bladder sensory neurons. Although there are several animal models of cystitis, none specifically relates to BPS. Here, we aimed to create such a model using enzymatic digestion of the barrier proteoglycans (PGs) in the rat. Twenty female Wistar rats were anaesthetized and transurethrally catheterized. Ten animals were treated with 0.25IU of intravesical chondroitinase ABC and heparanase III to digest chondroitin sulphate and heparin sulphate PGs, respectively...
January 2017: Pain
https://www.readbyqxmd.com/read/27917521/international-continence-society-good-urodynamic-practices-and-terms-2016-urodynamics-uroflowmetry-cystometry-and-pressure-flow-study
#17
REVIEW
Peter F W M Rosier, Werner Schaefer, Gunnar Lose, Howard B Goldman, Michael Guralnick, Sharon Eustice, Tamara Dickinson, Hashim Hashim
AIMS: The working group initiated by the ICS Standardisation Steering Committee has updated the International Continence Society Standard "Good Urodynamic Practice" published in 2002. METHODS: On the basis of the manuscript: "ICS standard to develop evidence-based standards," a new ICS Standard was developed in the period from December 2013 to December 2015. In July, a draft was posted on the ICS website for membership comments and discussed at the ICS 2015 annual meeting...
December 5, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27915481/effects-of-combination-treatment-of-alpha-1-adrenergic-receptor-antagonists-on-voiding-dysfunction-study-on-target-organs-in-overactive-bladder-rats
#18
Il Gyu Ko, Bo Min Moon, Sung Eun Kim, Jun Jang Jin, Lakkyong Hwang, Eun Sang Ji, Chang Ju Kim, Tai Hyung Kim, Hyun Hee Choi, Kyung Jin Chung
PURPOSE: Overactive bladder (OAB) causes urinary urgency, usually accompanied by frequency and nocturia. Alpha 1-adrenergic receptor (α1-AR) antagonists are known to improve lower urinary tract symptoms associated with OAB. The α1-AR antagonists constitute a variety of drugs according to the receptor subtype affinity. This study investigated the efficacy of tamsulosin, naftopidil, and a combination of the two on OAB rats. METHODS: The OAB rat model was induced by an intraperitoneal injection of cyclophosphamide for 14 days...
November 2016: International Neurourology Journal
https://www.readbyqxmd.com/read/27891659/ics-teaching-module-cystometry-basic-module
#19
REVIEW
Carlos Arturo Levi D'Ancona, Mario João Gomes, Peter F W M Rosier
AIMS: To summarize the evidence background for education of good urodynamic practice, especially cystometry. METHODS: A search was done in PubMed for the last 5 years of publications selecting only clinical studies, utilizing the following keywords: cystometry 133 articles and filling cystometry 53 articles. RESULTS: The evidence with regard to clinical setting and cystometry technique, as well as for catheters and transducers type, infused solution and patient position is presented with recommendations...
November 28, 2016: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/27889808/pharmacodynamic-evaluation-of-lys-5-meleu-9-nle-10-nka-4-10-prokinetic-effects-on-bladder-and-colon-activity-in-acute-spinal-cord-transected-and-spinally-intact-rats
#20
F Aura Kullmann, M Katofiasc, K B Thor, Lesley Marson
The purpose of this study was to determine feasibility of a novel therapeutic approach to drug-induced voiding after spinal cord injury (SCI) using a well-characterized, peptide, neurokinin 2 receptor (NK2 receptor) agonist, Lys(5), MeLeu(9), Nle(10)-NKA(4-10) (LMN-NKA). Cystometry and colorectal pressure measurements were performed in urethane-anesthetized, intact, and acutely spinalized female rats. Bladder pressure and voiding were monitored in response to intravenous LMN-NKA given with the bladder filled to 70% capacity...
February 2017: Naunyn-Schmiedeberg's Archives of Pharmacology
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