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https://www.readbyqxmd.com/read/29144195/extraction-purification-of-prostate-specific-antigen-psa-and-establishment-of-radioimmunoassay-system-as-a-diagnostic-tool-for-prostate-disorders
#1
Ahmed Sami Abu-Bakr El-Bayoumy, Akaber Tarek Hessien Keshta, Khaled Mohamed Sallam, Nahed Hassan Ebeid, Hatem Mohamed Elsheikh, Bsheer El-Sayed Bayoumy
This study aimed to provide an easy and effective method for extraction and purification of prostate specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases...
November 16, 2017: Journal of Immunoassay & Immunochemistry
https://www.readbyqxmd.com/read/29143487/nurses-implementation-of-ventilator-associated-pneumonia-prevention-guidelines-an-observational-study-in-jordan
#2
Sami M Aloush
BACKGROUND: Ventilator-associated pneumonia prevention guidelines from the Center for Disease Control and Prevention, the American Thoracic Society, and the Institute for Health Care and Improvement have been published to reduce the rate of ventilator-associated pneumonia in the clinical settings; however, nurses' compliance with these guidelines is still questionable. AIMS: The purpose of this study was to assess nurses' compliance with ventilator-associated pneumonia prevention guidelines and the factors that influence their compliance...
November 15, 2017: Nursing in Critical Care
https://www.readbyqxmd.com/read/29142987/prospective-study-of-routine-heparin-avoidance-hemodialysis-in-a-tertiary-acute-care-inpatient-practice
#3
Sami Safadi, Robert C Albright, John J Dillon, Amy W Williams, Fares Alahdab, Julie K Brown, Amanda L Severson, Walter K Kremers, Mary Ann Ryan, Marie C Hogan
Introduction: Extracorporeal circuit (EC) anticoagulation with heparin is a key advance in hemodialysis (HD), but anticoagulation is problematic in inpatients at risk of bleeding. We prospectively evaluated a heparin-avoidance HD protocol, clotting of the EC circuit (CEC), impact on dialysis efficiency, and associated risk factors in our acute care inpatients who required HD (January 17, 2014 to May 31, 2015). Methods: HD sessions without routine EC heparin were performed using airless dialysis tubing...
July 2017: KI Reports
https://www.readbyqxmd.com/read/29142787/sleep-apnea-symptoms-and-cardiovascular-disease-risks-among-haitian-medical-students
#4
Diana M Rosenthal, Donaldson F Conserve, Dodley Severe, Michaele A Gedeon, Ferdinand Zizi, Georges Casimir, Samy I McFarlane, Girardin Jean Louis
Sleep apnea is a prevalent sleep disorder that disproportionately affects blacks and has been previously studied among Caribbean-born blacks in Brooklyn, New York, but there has been negligible research in the Caribbean, specifically Haiti, and developing countries on this pressing health issue. A total of 373 medical students (mean age=20.6 years ± 2.3 years) from a medical school in Haiti participated in this study. Participants were administered a questionnaire assessing their sleep health and cardiovascular outcomes...
April 2017: Journal of Sleep Disorders & Therapy
https://www.readbyqxmd.com/read/29142562/drug-poisoning-and-associated-factors-in-western-saudi-arabia-a-five-year-retrospective-chart-review-2011-2016
#5
Sami H Alzahrani, Ali H Alqahtani, Fayssal Mostafa Farahat, Mohammed Abdel Galil Elnour, Jamil Bashawri
Objective: Drug poisoning is a globally common cause of emergency-room admissions. This study explores drug-poisoning prevalence patterns, associated risk factors (gender, age and exposure circumstances), and outcomes in western Saudi Arabia. Methods: Retrospective analysis of Clinical drug poisoning cases (2011-2016). The data were retrieved from the Saudi Ministry of Health's record and Patients' medical charts were analyzed. Results: The Ministry of Health received 1,474 reports of drug poisoning during 2011-2016...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29140308/synergistic-effect-of-fluorinated-and-n-doped-tio%C3%A2-nanoparticles-leading-to-different-microstructure-and-enhanced-photocatalytic-bacterial-inactivation
#6
Irena Milosevic, Amarnath Jayaprakash, Brigitte Greenwood, Birgit van Driel, Sami Rtimi, Paul Bowen
This work focuses on the development of a facile and scalable wet milling method followed by heat treatment to prepare fluorinated and/or N-doped TiO₂ nanopowders with improved photocatalytic properties under visible light. The structural and electronic properties of doped particles were investigated by various techniques. The successful doping of TiO₂ was confirmed by X-ray photoelectron spectroscopy (XPS), and the atoms appeared to be mainly located in interstitial positions for N whereas the fluorination is located at the TiO₂ surface...
November 15, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29138507/systems-pathology-by-multiplexed-immunohistochemistry-and-whole-slide-digital-image-analysis
#7
Sami Blom, Lassi Paavolainen, Dmitrii Bychkov, Riku Turkki, Petra Mäki-Teeri, Annabrita Hemmes, Katja Välimäki, Johan Lundin, Olli Kallioniemi, Teijo Pellinen
The paradigm of molecular histopathology is shifting from a single-marker immunohistochemistry towards multiplexed detection of markers to better understand the complex pathological processes. However, there are no systems allowing multiplexed IHC (mIHC) with high-resolution whole-slide tissue imaging and analysis, yet providing feasible throughput for routine use. We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six protein markers and nuclei, and automatic quantification and classification of hundreds of thousands of cells in situ in formalin-fixed paraffin-embedded tissues...
November 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29137759/ultrasonic-assisted-production-of-starch-nanoparticles-structural-characterization-and-mechanism-of-disintegration
#8
Sami Boufi, Sihem Bel Haaj, Albert Magnin, Frédéric Pignon, Marianne Impéror-Clerc, Gérard Mortha
In this paper, the disintegration of starch (waxy and standard starch) granules into nanosized particles under the sole effect of high power ultrasonication treatment in water/isopropanol is investigated, by using wide methods of analysis. The present work aims at a fully characterization of the starch nanoparticles produced by ultrasonication, in terms of size, morphology and structural properties, and the proposition of a possible mechanism explaining the top-down generation of starch nanoparticles (SNPs) via high intensity ultrasonication...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29137725/a-gait-retraining-system-using-augmented-reality-to-modify-footprint-parameters-effects-on-lower-limb-sagittal-plane-kinematics
#9
Sami Bennour, Baptiste Ulrich, Thomas Legrand, Brigitte M Jolles, Julien Favre
Improving lower-limb flexion/extension angles during walking is important for the treatment of numerous pathologies. Currently, these gait retraining procedures are mostly qualitative, often based on visual assessment and oral instructions. This study aimed to propose an alternative method combining motion capture and display of target footprints on the floor. The second objectives were to determine the error in footprint modifications and the effects of footprint modifications on lower-limb flexion/extension angles...
November 11, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29136363/dynamic-stabilization-of-the-ligand-metal-interface-in-atomically-precise-gold-nanoclusters-au68-and-au144-protected-by-meta-mercaptobenzoic-acid
#10
Tiia-Riikka Tero, Sami Malola, Benedek Koncz, Emmi Pohjolainen, Saara Lautala, Satu Mustalahti, Perttu Permi, Gerrit Groenhof, Mika Pettersson, Hannu Häkkinen
Ligand-stabilized, atomically precise gold nanoclusters with a metal core of a uniform size of just 1 - 3 nm constitute an interesting class of nanomaterials with versatile possibilities for applications due to their size dependent properties and modifiable ligand layers. The key to extending the usability of the clusters in applications is to understand the chemical bonding in the ligand layer as a function of cluster size and ligand structure. Previously, it has been shown that monodispersed gold nanoclusters, stabilized by meta-mercaptobenzoic acid (m-MBA or 3-MBA) ligands and with sizes of 68 to 144 gold atoms, show ambient stability...
November 14, 2017: ACS Nano
https://www.readbyqxmd.com/read/29134177/endovesical-leiomyoma-of-bladder-treated-by-enucleation-a-case-report
#11
Kays Chaker, Ahmed Sellami, Yassine Ouanes, Alia Zehani, Kheireddine Mrad Dali, Karem Abid, Sami Ben Rhouma, Yassine Nouira
No abstract text is available yet for this article.
January 2018: Urology Case Reports
https://www.readbyqxmd.com/read/29133083/jejunal-diverticulitis-review-and-treatment-algorithm
#12
Houssem Harbi, Nizar Kardoun, Sami Fendri, Nouha Dammak, Nozha Toumi, Ahmed Guirat, Rafik Mzali
Diverticulitis is much less common in the jejunum than in colonic diverticula probably because of diverticulum larger size, better intra-luminal flow and relatively sterile jejunal content. Complications of jejunal diverticulitis are acute intestinal obstruction, diverticular bleeding and mainly perforation with mesenteric abscess, localized or generalized peritonitis. The Multi-detector row computed tomography is now the best diagnostic imaging for small bowel diverticulitis as well as its complications. Magnetic resonance enterography is also quite useful but it is still impractical to resort to such an imaging in an abdominal emergency...
November 10, 2017: La Presse Médicale
https://www.readbyqxmd.com/read/29132688/temporal-trends-in-outpatient-management-of-incident-pulmonary-embolism-and-associated-mortality
#13
Adi J Klil-Drori, Janie Coulombe, Samy Suissa, Andrew Hirsch, Vicky Tagalakis
INTRODUCTION: In clinical trial settings, outpatient management of pulmonary embolism (PE) is feasible and safe, but less is known on its use in routine care. We determined trends in outpatient management of PE and associated mortality in a large non-select patient population. METHODS: All residents of Quebec, Canada with a first-ever work-up for suspected PE in the emergency department (ED) over 10years were included. Patients could transition to outpatient management and from unconfirmed to confirmed PE in a time-varying fashion...
November 6, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/29131355/interaction-of-albumins-and-heparinoids-investigated-by-affinity-capillary-electrophoresis-and-free-flow-electrophoresis
#14
Mona Mozafari, Sami El Deeb, Friederike Krull, Robert Wildgruber, Gerhard Weber, Christian G Reiter, Hermann Wätzig
A fast and precise affinity capillary electrophoresis (ACE) method has been applied to investigate the interactions between two serum albumins (HSA and BSA) and heparinoids. Furthermore, different free flow electrophoresis methods were developed to separate the species which appears owing to interaction of albumins with pentosan polysulfate sodium (PPS) under different experimental conditions. For ACE experiments, the normalized mobility ratios (∆R/Rf ), which provided information about the binding strength and the overall charge of the protein-ligand complex, were used to evaluate the binding affinities...
November 13, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29130420/-there-are-more-things-in-heaven-and-earth-how-knowledge-about-traditional-healing-affects-clinical-practice-interviews-with-conventional-health-personnel
#15
Anette Langås-Larsen, Anita Salamonsen, Agnete Egilsdatter Kristoffersen, Torunn Hamran, Bjørg Evjen, Trine Stub
People with Sami and Norwegian background are frequent users of traditional folk medicine (TM). Traditional healing, such as religious prayers of healing (reading) and the laying on of hands, are examples of commonly used modalities. The global aim of this study is to examine whether health personnel's knowledge, attitudes and experiences of traditional healing affect their clinical practice. Semi-structured individual interviews (n=32) and focus group interviews (n=2) were conducted among health personnel in two communities in Northern Norway...
2017: International Journal of Circumpolar Health
https://www.readbyqxmd.com/read/29129756/assessment-of-oncological-outcomes-after-radical-prostatectomy-according-to-preoperative-and-postoperative-cancer-of-the-prostate-risk-assessment-scores-results-from-a-large-two-center-experience
#16
Sami-Ramzi Leyh-Bannurah, Paolo Dell'Oglio, Emanuele Zaffuto, Alberto Briganti, Jonas Schiffmann, Raisa S Pompe, Derya Tilki, Hans Heinzer, Markus Graefen, Pierre I Karakiewicz, Lars Budäus
BACKGROUND: Among prostate cancer (PCa) patients undergoing radical prostatectomy (RP) and with virtually identical unfavorable pathological characteristics, those deemed at low risk (LR) preoperatively had better oncological outcomes than those with intermediate (IR) or high risk (HR) preoperatively. OBJECTIVE: To examine if this phenomenon still applies when preoperative Cancer of the Prostate Risk Assessment (CAPRA) scores are compared to postoperative scores (CAPRA-S) in RP patients...
November 9, 2017: European Urology Focus
https://www.readbyqxmd.com/read/29129733/doxorubicin-synergism-and-resistance-reversal-in-human-neuroblastoma-be-2-c-cell-lines-an-in-vitro-study-with-dextran-catechin-nanohybrids
#17
Orazio Vittorio, Marion Le Grand, Sami A Makharza, Manuela Curcio, Paola Tucci, Francesca Iemma, Fiore Pasquale Nicoletta, Silke Hampel, Giuseppe Cirillo
Hybrid nanocarrier consisting in nanographene oxide coated by a dextran-catechin conjugate was proposed in the efforts to find more efficient Neuroblastoma treatment with Doxorubicin chemotherapy. The dextran-catechin conjugate was prepared by immobilized laccase catalysis and its peculiar reducing ability exploited for the synthesis of the hybrid carrier. Raman spectra and DSC thermograms were recorded to check the physicochemical properties of the nanohybrid, while DLS measurements, SEM, TEM, and AFM microscopy allowed the determination of its morphological and dimensional features...
November 9, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29127633/natural-and-anthropogenic-particulate-bound-aliphatic-and-polycyclic-aromatic-hydrocarbons-in-surface-waters-of-the-gulf-of-gab%C3%A3-s-tunisia-southern-mediterranean-sea
#18
Rania Fourati, Marc Tedetti, Catherine Guigue, Madeleine Goutx, Hatem Zaghden, Sami Sayadi, Boubaker Elleuch
Particulate-bound aliphatic and polycyclic aromatic hydrocarbons (AHs and PAHs) were investigated in the surface waters of the Gulf of Gabès (Tunisia, southern Mediterranean Sea). Samples were collected off the Sfax and Gabès-Ghannouch coasts. Concentrations in total resolved n-alkanes ranged from 0.03 to 3.2 μg L(-1), and concentrations in total parents + alkylated PAHs ranged from bdl to 108.6 ng L(-1). The highest concentrations were recorded in the southern Sfax. AHs were mainly of biogenic origin with odd n-alkane predominance, although an anthropogenic contribution was also detected...
November 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29127269/intracerebral-hemorrhage-and-outcome-after-thrombolysis-in-stroke-patients-using-selective-serotonin-reuptake-inhibitors
#19
Jan F Scheitz, Guillaume Turc, Linda Kujala, Alexandros A Polymeris, Mirjam R Heldner, Thomas P Zonneveld, Hebun Erdur, Sami Curtze, Christopher Traenka, Céline Brenière, Roland Wiest, Andrea Rocco, Gerli Sibolt, Henrik Gensicke, Matthias Endres, Nicolas Martinez-Majander, Yannick Béjot, Paul J Nederkoorn, Catherine Oppenheim, Marcel Arnold, Stefan T Engelter, Daniel Strbian, Christian H Nolte
BACKGROUND AND PURPOSE: Selective serotonin-reuptake inhibitors (SSRIs) impair platelet function and have been linked to a higher risk of spontaneous intracerebral hemorrhage-an association that may be augmented by oral anticoagulants (OAC). We aimed to assess whether preadmission treatment with SSRIs in patients with acute ischemic stroke is associated with post-thrombolysis symptomatic intracerebral hemorrhage (sICH) and functional outcome. METHODS: A multicenter retrospective analysis was conducted in prospective registries of patients treated by thrombolysis within 4...
November 10, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/29126927/mechanisms-of-macular-edema-beyond-the-surface
#20
REVIEW
Alejandra Daruich, Alexandre Matet, Alexandre Moulin, Laura Kowalczuk, Michaël Nicolas, Alexandre Sellam, Pierre-Raphaël Rothschild, Samy Omri, Emmanuelle Gélizé, Laurent Jonet, Kimberley Delaunay, Yvonne De Kozak, Marianne Berdugo, Min Zhao, Patricia Crisanti, Francine Behar-Cohen
Macular edema consists of intra- or subretinal fluid accumulation in the macular region. It occurs during the course of numerous retinal disorders and can cause severe impairment of central vision. Major causes of macular edema include diabetes, branch and central retinal vein occlusion, choroidal neovascularization, posterior uveitis, postoperative inflammation and central serous chorioretinopathy. The healthy retina is maintained in a relatively dehydrated, transparent state compatible with optimal light transmission by multiple active and passive systems...
November 7, 2017: Progress in Retinal and Eye Research
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