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https://www.readbyqxmd.com/read/28924827/dual-contrast-ct-method-enables-diagnostics-of-cartilage-injuries-and-degeneration-using-a-single-ct-image
#1
Annina E A Saukko, Juuso T J Honkanen, Wujun Xu, Sami P Väänänen, Jukka S Jurvelin, Vesa-Pekka Lehto, Juha Töyräs
Cartilage injuries may be detected using contrast-enhanced computed tomography (CECT) by observing variations in distribution of anionic contrast agent within cartilage. Currently, clinical CECT enables detection of injuries and related post-traumatic degeneration based on two subsequent CT scans. The first scan allows segmentation of articular surfaces and lesions while the latter scan allows evaluation of tissue properties. Segmentation of articular surfaces from the latter scan is difficult since the contrast agent diffusion diminishes the image contrast at surfaces...
September 18, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28924280/antibiotics-use-and-misuse-in-upper-respiratory-tract-infection-patients-knowledge-attitude-and-practice-analysis-in-university-hospital-saudi-arabia
#2
Waseem Hajjar, Sami Alnassar, Sara Al-Khelb, Sarah Al-Mutairi, Norah Al-Refayi, Sultan Ayoub Meo
OBJECTIVE: To assess the awareness and causes of antibiotics use and misuse in patients with upper respiratory tract infections. METHODS: This cross-sectional study was conducted at King Saud University, Riyadh, Saudi Arabia, from January 2014 to December 2015, and comprised patients suffering from upper respiratory tract infections. A well-established, self-administrated questionnaire was randomly distributed to the patients. The questionnaire consisted of 3 parts: demographic variables, knowledge, and beliefs, and behaviour towards antibiotic use and misuse...
September 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28920958/the-n-6-methyladenosine-m-6-a-forming-enzyme-mettl3-controls-myeloid-differentiation-of-normal-hematopoietic-and-leukemia-cells
#3
Ly P Vu, Brian F Pickering, Yuanming Cheng, Sara Zaccara, Diu Nguyen, Gerard Minuesa, Timothy Chou, Arthur Chow, Yogesh Saletore, Matthew MacKay, Jessica Schulman, Christopher Famulare, Minal Patel, Virginia M Klimek, Francine E Garrett-Bakelman, Ari Melnick, Martin Carroll, Christopher E Mason, Samie R Jaffrey, Michael G Kharas
N(6)-methyladenosine (m(6)A) is an abundant nucleotide modification in mRNA that is required for the differentiation of mouse embryonic stem cells. However, it remains unknown whether the m(6)A modification controls the differentiation of normal and/or malignant myeloid hematopoietic cells. Here we show that shRNA-mediated depletion of the m(6)A-forming enzyme METTL3 in human hematopoietic stem/progenitor cells (HSPCs) promotes cell differentiation, coupled with reduced cell proliferation. Conversely, overexpression of wild-type METTL3, but not of a catalytically inactive form of METTL3, inhibits cell differentiation and increases cell growth...
September 18, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28919908/hepatic-s6k1-partially-regulates-lifespan-of-mice-with-mitochondrial-complex-i-deficiency
#4
Takashi K Ito, Chenhao Lu, Jacob Khan, Quy Nguyen, Heather Z Huang, Dayae Kim, James Phillips, Jo Tan, Yenna Lee, Tuyet Nguyen, Samy Khessib, Natalie Lim, Surapat Mekvanich, Joshua Oh, Victor V Pineda, Weirong Wang, Alessandro Bitto, Jonathan Y An, John F Morton, Mitsutoshi Setou, Warren C Ladiges, Matt Kaeberlein
The inactivation of ribosomal protein S6 kinase 1 (S6K1) recapitulates aspects of caloric restriction and mTORC1 inhibition to achieve prolonged longevity in invertebrate and mouse models. In addition to delaying normative aging, inhibition of mTORC1 extends the shortened lifespan of yeast, fly, and mouse models with severe mitochondrial disease. Here we tested whether disruption of S6K1 can recapitulate the beneficial effects of mTORC1 inhibition in the Ndufs4 knockout (NKO) mouse model of Leigh Syndrome caused by Complex I deficiency...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28919671/detection-of-staphylococcus-aureus-enterotoxigenic-strains-in-bovine-raw-milk-by-reversed-passive-latex-agglutination-and-multiplex-polymerase-chain-reaction
#5
Asmaa Samy Mansour, Gad El-Said Wagih, Sabry D Morgan, Mahmoud Elhariri, Mona A El-Shabrawy, Azza S M Abuelnaga, E A Elgabry
AIM: This review gives an outline of the assessment of enterotoxigenic Staphylococcus aureus tainting levels in raw milk from different sources in Egypt and characterization of enterotoxigenic strains utilizing a technique in light of PCR to identify genes coding for the production of staphylococcal enterotoxin (SE). The obtained data were compared with results from the application of the reversed passive latex. MATERIALS AND METHODS: Multiplex PCR and reversed passive latex agglutination (RPLA) were used...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28918346/anaerobic-digestion-for-bioenergy-production-global-status-environmental-and-techno-economic-implications-and-government-policies
#6
REVIEW
Juliana Vasco-Correa, Sami Khanal, Ashish Manandhar, Ajay Shah
Anaerobic digestion (AD) is a mature technology that can transform organic matter into a bioenergy source - biogas (composed mainly of methane and carbon dioxide), while stabilizing waste. AD implementation around the world varies significantly, from small-scale household digesters in developing countries to large farm-scale or centralized digesters in developed countries. These differences in the implementation of AD technology are due to a complex set of conditions, including economic and environmental implications of the AD technology, and stimulus provided by a variety of polices and incentives related to agricultural systems, waste management, and renewable energy production...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28918060/outcomes-of-ahmed-glaucoma-valve-revision-in-pediatric-glaucoma
#7
Ahmed Mansour Al-Omairi, Aliah H Al Ameri, Sami Al-Shahwan, Arif O Khan, Ibrahim Al-Jadaan, Ahmed Mousa, Deepak P Edward
PURPOSE: Encapsulation of the Ahmed glaucoma valve (AGV) plate is a common cause for postoperative elevation of intraocular pressure, especially in children. Many reports have described the outcomes of AGV revision in adults. However, the outcomes of AGV revision in children are poorly documented. The aim of this study was to determine the outcomes of AGV revision in children. DESIGN: Retrospective cross sectional study METHODS: A retrospective chart review of patients less than 15 years of age who underwent AGV revision with a minimum postoperative follow-up of six months was conducted...
September 13, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/28917486/effects-of-the-potential-probiotics-bacillus-aerophilus-kadr3-in-inducing-immunity-and-disease-resistance-in-labeo-rohita
#8
Dharmaraj Ramesh, Sami Souissi, Tharifkhan Shan Ahamed
The aim of the present study was to evaluate the dietary administration of Bacillus aerophilus KADR3 on enhancing the immunity and disease resistance against Aeromonas hydrophila infection in Labeo rohita. B. aerophilus culture supernatant exhibited antagonistic activity against fish pathogenic bacteria in an agar well diffusion assay. Four fish groups were fed with either of following diets containing different concentrations of B. aerophilus KADR3 (cfu g(-1)): 0 (control), 1 × 10(7) (DI), 1 × 10(8) (DII), and 1 × 10(9) (DIII) -cfu g(-1)...
September 13, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28914427/the-clinical-and-genetic-spectrum-of-maroteaux-lamy-syndrome-mucopolysaccharidosis-vi-in-the-eastern-province-of-saudi-arabia
#9
Nouriya Abbas Al-Sannaa, Hind Yousif Al-Abdulwahed, Sami Ibrahim Al-Majed, Issam Hassan Bouholaigah
Mucopolysaccharidosis (MPS VI) or Maroteaux-Lamy syndrome is an autosomal recessive lysosomal storage disease caused by deficiency of the enzyme N-acetylgalactosamine 4-sulfatase or arylsulfatase B. It is involved in the degradation of glycosaminoglycans and characterized by a wide spectrum of clinical and genetic heterogeneity. So far, more than 150 mutations have been reported in the ARSB gene. Most of these mutations are either novel, private, or compound heterozygous making phenotype-genotype correlation as well as population screening difficult...
September 15, 2017: Journal of Community Genetics
https://www.readbyqxmd.com/read/28913133/the-relationship-between-fecal-incontinence-and-vaginal-delivery-in-the-postmenopausal-stage
#10
Süleyman Kargın, Sami Çifçi, Adnan Kaynak, Hüseyin Ataseven, Cengiz Kadıyoran, Murat Çakır
OBJECTIVE: Obstetric anal sphincter injuries are one of the most significant complications of vaginal delivery that give way to fecal incontinence, which is defined as the involuntary leakage of gas, fluid or solid stool. Although sphincter injuries are seen in 0.5-9% of all deliveries. It has been reported that 20-41% of women who had vaginal deliveries had occult anal sphincter injuries as endoanal ultrasonography began to be used by physicians. The aim of our study was to investigate the relationship between fecal incontinence, whose incidence increases dramatically during the postmenopausal stage, and occult anal sphincter injuries...
March 2017: Turk J Obstet Gynecol
https://www.readbyqxmd.com/read/28912268/structural-and-functional-diversity-in-listeria-cell-wall-teichoic-acids
#11
Yang Shen, Samy Boulos, Eric Sumrall, Benjamin Gerber, Alicia Julian-Rodero, Marcel R Eugster, Lars Fieseler, Laura Nyström, Marc-Olivier Ebert, Martin J Loessner
Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we employed a combination of UPLC-MS/MS and NMR to determine the structural complexity of WTAs from Listeria species. We unveiled more than 10 different types of WTA polymers that vary in their linkage and repeating units...
September 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28911807/cellulose-nanofibrils-cnfs-from-ammophilaarenaria-a-natural-and-a-fast-growing-grass-plant
#12
Zayneb Jebali, Abdelkader Nabili, Hatem Majdoub, Sami Boufi
The production of cellulose nanofibrils (CNFs) with a high yield from Ammophilaarenariahas been explored and the ensuing CNFs were characterized in terms of morphology, crystallinity and rheological properties. Ammophilaarenaria (marram grass) is a perennial grass plant with high tolerance to drought, which is growing naturally in nutritionally poor sand with low organic matter. Fibers were extracted from the plant by conventional alkaline pulping and bleaching with a yield around 50% based on the dry weight of the plant...
September 11, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28910428/retinal-indentation-by-a-dexamethasone-implant-in-a-gas-filled-eye-report-of-an-unusual-complication
#13
Sami H Uwaydat, Ahmed B Sallam, Hans Wang, Sunali Goyal
No abstract text is available yet for this article.
September 14, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/28910094/hemicellulosic-polysaccharides-mimics-synthesis-of-tailored-bottlebrush-like-xyloglucan-oligosaccharide-glycopolymers-as-binders-of-nanocrystalline-cellulose
#14
Jing Chen, Christophe Travelet, Redouane Borsali, Sami Halila
We report in this contribution that while low molecular weight hemicellulosic building blocks are known not to interact with cellulosic materials, their multivalent presentation on a polymeric scaffold significantly enhanced the binding interactions that are remarkably in the same range as those usually observed for lectin-carbohydrate interactions. We developed a poly(propargyl methacrylate) scaffold on which we conjugated, by "post-click" reaction, a variety of azide reducing-end functionalized xyloglucan oligosaccharides with controlled enzymatic-mediated rate of degalactosylation...
September 14, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28906319/enteroaggregative-e-coli-subclinical-infection-and-co-infections-and-impaired-child-growth-in-the-mal-ed-cohort-study
#15
Aldo A M Lima, Alberto M Soares, José Q S Filho, Alexandre Havt, Ila F N Lima, Noélia L Lima, Cláudia B Abreu, Francisco S Junior, Rosa M S Mota, William K-Y Pan, Christopher Troeger, Pedro H Q S Medeiros, Herlice N Vera, Mara M G Prata, Ben McCormick, Monica McGrath, Elizabeth Rogawski, Eric Houpt, James Platts-Mills, Jean Gratz, Amidou Samie, Pascal Bessong, Sudhir Babji, Gangadeep Kang, Qureshi Shahida, Sadia Shakoor, Zulfiqar Bhutta, Rashidul Haque, Tahmeed Ahmed, Estomih Mduma, Erling Svensen, Margaret Kosek, Pablo Penataro Yori, Ladaporn Bodhidatta, Shrestha Jasmin, Carl Mason, Dennis Lang, Michael Gottlieb, Richard L Guerrant
OBJECTIVE: We evaluated the impact of subclinical enteroaggregative Escherichia coli (EAEC) infection alone and in combination with other pathogens in the first six months of life on child growth. METHODS: Non-diarrheal samples from 1,684 children across eight Multisite Birth Cohort Study, Malnutrition and Enteric Diseases (MAL-ED) sites in Asia, Africa, and Latin America were tested monthly; over 90% of children were followed-up twice weekly for the first six months of life...
September 12, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28906114/doglycans-tools-for-preparing-carbohydrate-structures-for-atomistic-simulations-of-glycoproteins-glycolipids-and-carbohydrate-polymers-with-gromacs
#16
Reinis Danne, Chetan Poojari, Hector Martinez-Seara, Sami Rissanen, Fabio Lolicato, Tomasz Rog, Ilpo Vattulainen
Carbohydrates constitute a structurally and functionally diverse group of biological and macromolecules. In cells they are involved in, e.g., energy storage, signaling, and cell-cell recognition. All of these phenomena take place in atomistic scales, thus atomistic simulation would be the method of choice to explore how carbohydrates function. However, the progress in the field is limited by the lack of appropriate tools for preparing carbohydrate structures and related topology files for the simulation models...
September 14, 2017: Journal of Chemical Information and Modeling
https://www.readbyqxmd.com/read/28906003/inclusion-of-mers-spike-protein-elisa-in-algorithm-to-determine-serologic-evidence-of-mers-cov-infection
#17
Suvang Trivedi, Congrong Miao, Mohammad Mousa Al-Abdallat, Aktham Haddadin, Sultan Alqasrawi, Ibrahim Iblan, Mohannad Al Nsour, Tarek Alsanouri, Sami Sheikh Ali, Brian Rha, Susan I Gerber, Daniel C Payne, Azaibi Tamin, Natalie J Thornburg
The Centers for Disease Control and Prevention (CDC) algorithm for detecting presence of serum antibodies against Middle East Respiratory Syndrome coronavirus (MERS-CoV) in subjects with potential infections with the virus has included screening by indirect ELISA against recombinant nucleocapsid (N) protein and confirmation by immunofluorescent staining of infected monolayers and/or microneutralization titration. Other international groups include indirect ELISA assays using the spike (S) protein, as part of their serological determinations...
September 14, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28902425/efficient-catalytic-microreactors-with-atomic-layer-deposited-platinum-nanoparticles-on-oxide-support
#18
Ville Rontu, Anne Selent, Vladimir Zhivonitko, Gianmario Scotti, Igor Koptyug, Ville-Veikko Telkki, Sami Franssila
Microreactors attract a significant interest for chemical synthesis due to benefits of small scales such as high surface to volume ratio, rapid thermal ramping and well-understood laminar flows. The suitability of atomic layer deposition for applying of both the catalyst nanoparticles and the support material on the surfaces of channels of microfabricated silicon microreactors is demonstrated in this research. Continuous flow hydrogenation of propene into propane at low temperatures with TiO₂ supported catalytic Pt nanoparticles was used as a model reaction...
September 13, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28899686/the-anti-c1s-antibody-tnt003-prevents-complement-activation-in-the-skin-induced-by-bullous-pemphigoid-autoantibodies
#19
Anika Kasprick, Maike M Holtsche, Eileen L Rose, Sami Hussain, Enno Schmidt, Frank Petersen, Sandip Panicker, Ralf J Ludwig
No abstract text is available yet for this article.
September 9, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28899591/robust-assessment-of-both-biochemical-methane-potential-and-degradation-kinetics-of-solid-residues-in-successive-batches
#20
Mokhles Kouas, Michel Torrijos, Philippe Sousbie, Jean-Philippe Steyer, Sami Sayadi, Jérôme Harmand
The well-known batch assay test is used worldwide to determine the biochemical methane potential (BMP) of solid substrates in a single batch but its use to estimate the degradation kinetics may lead to underestimations. To overcome this problem, a different approach was carried out to characterize simultaneously both BMP of solid substrates and their degradation kinetics in successive batches, i.e. after an acclimation period. In a second step, a simple model was developed based on the methane production curve in batch mode for dividing the organic matter of the substrate into three sub-fractions according to their degradation rates (rapid, moderate and slow)...
September 9, 2017: Waste Management
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