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Antibiofilm activity

Carlos Rumbo, Juan Antonio Tamayo-Ramos, M Federica Caso, Antonio Rinaldi, Lorena Romero-Santacreu, Roberto Quesada, Santiago Cuesta
Electrospun biodegradable polymers have emerged as promising materials for their applications in several fields, including biomedicine and food industry. For this reason, the susceptibility of these materials to be colonized by different pathogens is a critical issue for public health, and their study can provide future knowledge to develop new strategies against bacterial infections. In this work, the ability of three pathogenic bacterial species ( Pseudomonas aeruginosa, Acinetobacter baumannii and Listeria monocytogenes) to adhere and form biofilm in electrospun polycaprolactone (PCL) microfibrous meshes was investigated...
March 20, 2018: ACS Applied Materials & Interfaces
Josy Goldoni Lazarini, Janaina de Cássia Orlandi Sardi, Marcelo Franchin, Bruno Dias Nani, Irlan Almeida Freires, Juliana Infante, Jonas Augusto Rizzato Paschoal, Severino Matias de Alencar, Pedro Luiz Rosalen
The anti-inflammatory and antibiofilm activities as well as toxicity and chemical profile of Eugenia brasiliensis pulp extract (EBE), were evaluated. EBE chemical profile and phenolic content were determined by LC-MS/MS. EBE was tested for its in vitro and in vivo anti-inflammatory activity, including TNF-α release, NF-кB activation, neutrophil migration and paw edema. The MIC/MBC and antibiofilm activities were tested against methicillin sensitive and resistant Staphylococcus aureus, Escherichia coli, Pseudomona aeruginosa, Streptococcus mutans, and Lactobacillus acidophilus...
March 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Roberto Scaffaro, Francesco Lopresti, Manuela D'Arrigo, Andreana Marino, Antonia Nostro
Carvacrol (CAR) is one of the most promising essential oil components with antimicrobial activity. New technologies aimed to incorporate this active molecule into carrier matrix to improve the stability and prolong the biological activity. The goal of this study was to investigate the feasibility of incorporating CAR into electrospun membranes of poly(lactic acid) (PLA) for potential applications as active antimicrobial system. To this end, PLA membranes containing homogeneously dispersed CAR were successfully prepared and a series of systematic tests including morpho-mechanical properties, in vitro release rate, and antimicrobial/antibiofilm activities against Staphylococcus aureus and Candida albicans were carried out...
March 13, 2018: Applied Microbiology and Biotechnology
Govindan Rajivgandhi, Ramachandran Vijayan, Muthuchamy Maruthupandy, Baskaralingam Vaseeharan, Natesan Manoharan
Urinary tract infections (UTIs) are a diverse public health complication and are caused by range of pathogens, however most often by Gram negative bacteria which cause significant life threatening risks to different populations. The prevalence rate and antimicrobial resistance among the Gram negative uropathogens alarmed to significantly heighten the economic burden of these infections. In this study, we investigated the role of Pyrrolo[1,2-a] pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl) on endophytic actinomycetes Nocardiopsis sp...
March 7, 2018: Microbial Pathogenesis
Sangily Jayanthi, Baskaralingam Vaseeharan, Ramachandran Ishwarya, Sivashanmugam Karthikeyan, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Csaba Vágvölgyi
Prophenoloxidase is a conserved Cu-containing enzyme acting as a major defense molecule in the immune response of crustaceans. In the present research, we purified prophenoloxidase from the haemolymph of Portunus pelagicus (Pp-proPO) by Blue Sepharose CL-6B chromatography. Pp-proPO exhibited only one band with molecular weight of 75 kDa on SDS-PAGE. The purified Pp-proPO was characterized through X-ray diffraction (XRD) and high-performance liquid chromatography (HPLC). Pp-proPO showed phagocytic activity on the yeast Saccharomyces cerevisiae as well as encapsulation on sepharose CL-6B beads associated with CM sepharose and beads of sodium alginate...
March 7, 2018: International Journal of Biological Macromolecules
Abderrahmen Merghni, Emira Noumi, Ons Hadded, Neyla Dridi, Harsh Panwar, Ozgur Ceylan, Maha Mastouri, Mejdi Snoussi
Antibacterial resistance is an increasingly serious threat to global public health. The search for new anti-infection agents from natural resources, with different mode of actions and competitive effects became a necessity. In this study, twenty height methicillin-resistant Staphylococcus aureus (MRSA) strains were investigated for their biofilm formation ability. Subsequently, the antibiofilm effects of Eucalyptus globulus essential oil and its main component 1,8-cineole, against MRSA, as well as their antiquorum sensing potential, were evaluated...
March 6, 2018: Microbial Pathogenesis
Pan Cao, Ying Yang, Fidelia Ijeoma Uche, Sarah Ruth Hart, Wen-Wu Li, Chengqing Yuan
Modification of metal surfaces with antimicrobial peptides is a promising approach to reduce bacterial adhesion. Here, cyclic peptides or cycloids, possessing remarkable stability and antimicrobial activities, were extracted and purified from Viola philippica Cav., and identified using mass spectrometry. Cyclotides were subsequently utilized to modify stainless steel surfaces via polydopamine-mediated coupling. The resulting cyclotide-modified surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy and contact angle analysis...
March 9, 2018: International Journal of Molecular Sciences
Mahalingam Anjugam, Baskaralingam Vaseeharan, Arokiadhas Iswarya, Narayanan Gobi, Mani Divya, Merlin P Thangaraj, Preetham Elumalai
Recently, several immunostimulants such as β-glucan, microbial and plant products have been used as dietary supplements to combat disease outbreaks in aquaculture. The present study investigates the potential of Portunus pelagicus β-1, 3 glucan binding protein based zinc oxide nanoparticles (Ppβ-GBP-ZnO NPs) supplemented diet on growth, immune response and disease resistance in Mozambique tilapia, Oreochromis mossambicus. The immune-related protein β-GBP was purified from the haemolymph of P. pelagicus using Sephadex G-100 affinity column chromatography...
March 5, 2018: Fish & Shellfish Immunology
Sara H Mohamed, Mahmoud S M Mohamed, Mary S Khalil, Magda Azmy, Mona I Mabrouk
AIM: This study aimed to test biofilm inhibition activities of each of essential oils (EOs), main compounds of EOs and enzymes against pathogenic Klebsiella pneumoniae METHODS AND RESULTS: Effect of seven EOs and three enzymes was tested on formation and eradication of Klebsiella pneumoniae biofilm. Peppermint oil showed robust biofilm inhibitory effect, causing inhibition ranged from 69.2-98.2% at 5 μl ml-1 . Thyme oil was found to have the best biofilm-eradication ability, causing eradication ranged from 80...
March 8, 2018: Journal of Applied Microbiology
Fariza Abrão, Jessica A Alves, Gessica Andrade, Pollyanna F de Oliveira, Sérgio R Ambrósio, Rodrigo C S Veneziani, Denise C Tavares, Jairo K Bastos, Carlos H G Martins
This study evaluates the antibacterial activity of the Copaifera duckei Dwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell line. MIC and MBC assays pointed out the most promising metabolites for further studies on bactericidal kinetics, antibiofilm activity, and synergistic antibacterial action. The oleoresin and polyalthic acid but not eperu-8(20)-15,18-dioic provided encouraging MIC and MBC results at concentrations lower than 100 μg mL-1 ...
2018: Frontiers in Microbiology
Simona Arena, Andrea Scaloni
Hen egg is a raw material widely used for preparation of food, pharmaceutical and cosmetoceutical products. Dedicated proteomic studies on egg fractions identified most abundant proteins. No similar studies have been performed at the peptidomic level so far. This study fills this gap and provides a comprehensive picture of the peptides present in the yolk plasma of different hen egg types after 24 and 264 h of laying, using a two-step fractionation followed by combined MALDI-TOF-TOF-MS and nanoLC-ESI-Q-Orbitrap-MS/MS analysis...
March 7, 2018: Journal of Agricultural and Food Chemistry
Jonatas Rafael de Oliveira, Kely Karina Belato, Felipe Eduardo de Oliveira, Antonio Olavo Cardoso Jorge, Samira Esteves Afonso Camargo, Luciane Dias de Oliveira
This study evaluated the in vitro antibiofilm effect of 5 different commercial mouthwashes (Cepacol Traditional, Colgate Plax Fresh Mint, Listerine Cool Mint, Oral-B Complete, and Sensodyne) on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans, Escherichia coli, and Pseudomonas aeruginosa. The cytotoxic effect of the mouthwashes on gingival fibroblasts was also analyzed. A colorimetric assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to investigate the viability of biofilms after 48 hours and gingival fibroblasts after 24 hours...
March 2018: General Dentistry
T Nuryastuti, S Setiawati, N Ngatidjan, M Mustofa, J Jumina, D Fitriastuti, M I Darussalam
The therapy for invasive candidiasis related to biofilms infection remains a difficult medical problem. To overcome this problem, efforts have been made to search for novel antibiofilm agents from various sources. This study investigated the in vitro antibiofilm activity of (1)-N-2-methoxybenzyl-1,10-phenanthrolinium bromide (FEN) against Candida albicans. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm reduction concentration (MBRC) were determined using the MTT (3-(4-5-dimethylthiazol-2-yl)2,5-dyphenyl tetrazolium bromide) reduction assay...
March 3, 2018: Journal de Mycologie Médicale
Sami A Alyahya, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Ramzi A Mothana, Mohammed N Al-Anbr, Baskaralingam Vaseeharan, Ramachandran Ishwarya, Mariappan Yazhiniprabha, Giovanni Benelli
Recent research in entomology and parasitology focused on the efficacy of green fabricated nanomaterials as novel insecticides. In this study, we synthesized poly-dispersed and stable silver nanoparticles (AgNPs) using the leaf extract of Holostemma ada-kodien. The nanostructures were characterized by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction analysis. The efficacy of H...
February 17, 2018: Journal of Photochemistry and Photobiology. B, Biology
Chirom Aarti, Ameer Khusro, Rakesh Varghese, Mariadhas Valan Arasu, Paul Agastian, Naïf Abdullah Al-Dhabi, Soundharrajan Ilavenil, Ki Choon Choi
OBJECTIVE: To investigate the probiotic characteristics, anti-Candida activity, and antibiofilm attributes of Hentak derived Lactobacillus pentosus strain LAP1. DESIGN: The probiotic properties of strain LAP1 was depicted by adapting standard protocols. The anti-Candida and antibiofilm properties of isolate were determined using agar well diffusion assay and ELISA reader test, respectively. The time-kill assay was performed using viable colony count assay. Further, the co-aggregation property of strain LAP1 was determined based on standard methodology...
February 23, 2018: Archives of Oral Biology
Lorenzo Drago, Marco Toscano, Marta Bottagisio
Bone defects caused by trauma or pathological events are major clinical and socioeconomic burdens. Thus, the efforts of regenerative medicine have been focused on the development of non-biodegradable materials resembling bone features. Consequently, the use of bioactive glass as a promising alternative to inert graft materials has been proposed. Bioactive glass is a synthetic silica-based material with excellent mechanical properties able to bond to the host bone tissue. Indeed, when immersed in physiological fluids, bioactive glass reacts, developing an apatite layer on the granule's surface, playing a key role in the osteogenesis process...
February 24, 2018: Materials
Mariane Beatriz Sordi, Thaís Altoé Moreira, Juan Felipe Dumes Montero, Luis Cláudio Barbosa, César Augusto Magalhães Benfatti, Ricardo de Souza Magini, Andréa de Lima Pimenta, Júlio César Matias de Souza
Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. OBJECTIVE: To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. MATERIAL AND METHODS: We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine...
2018: Journal of Applied Oral Science: Revista FOB
Inês Santos Ferreira, Judith Kikhney, Laura Kursawe, Stefanie Kasper, Lídia M D Gonçalves, Andrej Trampuz, Annette Moter, Ana Francisca Bettencourt, António J Almeida
Eradication of Gram-positive biofilms is a critical aspect in implant-associated infection treatment. Although antibiotic-containing particulate carriers may be a promising strategy for overcoming biofilm tolerance, the assessment of their interaction with biofilms has not been fully explored. In the present work, the antibiofilm activity of daptomycin- and vancomycin-loaded poly(methyl methacrylate) (PMMA) and PMMA-Eudragit RL 100 (EUD) microparticles against methicillin-resistant Staphylococcus aureus (MRSA) and polysaccharide intercellular adhesin-positive S...
February 27, 2018: AAPS PharmSciTech
Srinivasan Ramanathan, Kannappan Arunachalam, Sivasankar Chandran, Rathika Selvaraj, Karutha Pandian Shunmugiah, Veera Ravi Arumugam
AIMS: This study aimed to evaluate the antibiofilm potential of phytol and cefotaxime combinations (PCCs) against A. baumannii and to elucidate the molecular mechanism of their antibiofilm potential through the transcriptomic approach. METHODS AND RESULTS: Phytol and cefotaxime combination(s) (PCC(s) [160 μg ml-1 + 8 μg ml-1 for microbial type culture collection (MTCC) strain and 160 μg ml-1 + 0.5 μg ml-1 for clinical isolate] effectively inhibited the A. baumannii biofilm formation...
February 23, 2018: Journal of Applied Microbiology
Marco Artini, Alexandros Patsilinakos, Rosanna Papa, Mijat Božović, Manuela Sabatino, Stefania Garzoli, Gianluca Vrenna, Marco Tilotta, Federico Pepi, Rino Ragno, Laura Selan
Pseudomonas aeruginosa is a ubiquitous organism and opportunistic pathogen that can cause persistent infections due to its peculiar antibiotic resistance mechanisms and to its ability to adhere and form biofilm. The interest in the development of new approaches for the prevention and treatment of biofilm formation has recently increased. The aim of this study was to seek new non-biocidal agents able to inhibit biofilm formation, in order to counteract virulence rather than bacterial growth and avoid the selection of escape mutants...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
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