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3D tissue culture

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https://www.readbyqxmd.com/read/27909243/3d-culture-models-of-tissues-under-tension
#1
REVIEW
Jeroen Eyckmans, Christopher S Chen
Cells dynamically assemble and organize into complex tissues during development, and the resulting three-dimensional (3D) arrangement of cells and their surrounding extracellular matrix in turn feeds back to regulate cell and tissue function. Recent advances in engineered cultures of cells to model 3D tissues or organoids have begun to capture this dynamic reciprocity between form and function. Here, we describe the underlying principles that have advanced the field, focusing in particular on recent progress in using mechanical constraints to recapitulate the structure and function of musculoskeletal tissues...
December 1, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27905868/short-term-effects-of-low-let-radiation-on-the-endothelial-barrier-uncoupling-of-pecam-1-and-the-production-of-endothelial-microparticles
#2
Thomas Templin, Preety Sharma, Peter Guida, Peter Grabham
A significant target for radiation-induced effects is the microvascular system, which is critical to healthy tissue function and its pathology is linked to disrupted endothelial barrier function. Low-linear energy transfer (LET) ionizing radiation is a source of noncancer pathologies in humans and little is known about the early events that could initiate subsequent diseases. However, it is well known that gamma radiation causes a very early disruption of the endothelial barrier at doses below those required for cytotoxic effects...
December 1, 2016: Radiation Research
https://www.readbyqxmd.com/read/27905026/native-matrix-based-human-lung-alveolar-tissue-model-in-vitro-studies-of-the-reparatory-actions-of-mesenchymal-stem-cells
#3
Ieva Bruzauskaite, Jovile Raudoniute, Jaroslav Denkovskij, Edvardas Bagdonas, Sandra Meidute-Abaraviciene, Vaida Simonyte, Daiva Bironaite, Almantas Siaurys, Eiva Bernotiene, Ruta Aldonyte
Studies of lung diseases in vitro often rely on flat, plastic-based monocultures, due to short lifespan of primary cells, complicated anatomy, lack of explants, etc. We hereby present a native 3D model with cues for repopulating epithelial cells. Abilities of mesenchymal stem cells (MSC) to modulate bacterial lipopolysaccharide (LPS) and cigarette smoke-induced injury to pulmonary epithelium were tested in our model. Post-mortem human lung tissue was sliced, cut and decellularized. Resulting matrix pads were reseeded with pulmonary epithelium (A549 line)...
December 1, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27903131/hypoxic-expansion-of-human-mesenchymal-stem-cells-enhances-3d-maturation-of-tissue-engineered-intervertebral-discs
#4
Katherine Deline Hudson, Lawrence J Bonassar
Culture of three dimensional (3D) constructs in hypoxic conditions (1-5% O2) has been shown to increase production of extracellular matrix (ECM) components in primary intervertebral disc (IVD) cells, and drive chondrogenesis of human mesenchymal stem cells (MSCs). Growing evidence that suggests that two dimensional (2D) expansion under hypoxic conditions may have an even greater influence on chondrogenesis in MSCs. This study aims to determine the effects of hypoxia during 2D expansion and subsequent 3D culture on the in vitro maturation of tissue engineering IVDs (TE-IVDs) made with human MSCs, using a previously developed TE-IVD system...
November 30, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27902740/extensive-characterization-and-comparison-of-endothelial-cells-derived-from-dermis-and-adipose-tissue-potential-use-in-tissue-engineering
#5
Hanneke N Monsuur, Ester M Weijers, Frank B Niessen, Amit Gefen, Pieter Koolwijk, Susan Gibbs, Lenie J van den Broek
Tissue-engineered constructs need to become quickly vascularized in order to ensure graft take. One way of achieving this is to incorporate endothelial cells (EC) into the construct. The adipose tissue stromal vascular fraction (adipose-SVF) might provide an alternative source for endothelial cells as adipose tissue can easily be obtained by liposuction. Since adipose-EC are now gaining more interest in tissue engineering, we aimed to extensively characterize endothelial cells from adipose tissue (adipose-EC) and compare them with endothelial cells from dermis (dermal-EC)...
2016: PloS One
https://www.readbyqxmd.com/read/27901089/a-silac-based-method-for-quantitative-proteomic-analysis-of-intestinal-organoids
#6
Alexis Gonneaud, Christine Jones, Naomie Turgeon, Dominique Lévesque, Claude Asselin, François Boudreau, François-Michel Boisvert
Organoids have the potential to bridge 3D cell culture to tissue physiology by providing a model resembling in vivo organs. Long-term growing organoids were first isolated from intestinal crypt cells and recreated the renewing intestinal epithelial niche. Since then, this technical breakthrough was applied to many other organs, including prostate, liver, kidney and pancreas. We describe here how to apply a SILAC-based quantitative proteomic approach to measure protein expression changes in intestinal organoids under different experimental conditions...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27900887/toward-modeling-the-human-nervous-system-in-a-dish-recent-progress-and-outstanding-challenges
#7
Barbora Vagaska, Patrizia Ferretti
Studying the cellular and molecular bases governing development, and normal and abnormal functions of the human CNS is hampered by its complexity and the very limited possibility of experimentally manipulating it in vivo. Development of 3D, tissue-like culture systems offers much promise for boosting our understanding of human neural development, birth defects, neurodegenerative diseases and neural injury, and for providing platforms that will more accurately predict efficacy of putative therapeutic compounds and assess responses to potentially neurotoxic agents...
November 30, 2016: Regenerative Medicine
https://www.readbyqxmd.com/read/27899064/gdf5-mediated-enhancement-of-chondrocyte-phenotype-is-inhibited-by-heparin-implication-for-the-use-of-heparin-in-the-clinic-and-in-tissue-engineering-applications
#8
Bethanie Imogen Ayerst, Raymond A A Smith, Victor Nurcombe, Anthony J Day, Catherine L R Merry, Simon Cool
The highly sulfated glycosaminoglycan (GAG) heparin is widely used in the clinic as an anticoagulant, and researchers are now using it to enhance stem cell expansion/ differentiation protocols, as well as to improve the delivery of growth factors for tissue engineering strategies. Growth differentiation factor 5 (GDF5) belongs to the bone morphogenetic protein family of proteins and is vital for skeletal formation, however, its interaction with heparin and heparan sulfate (HS) has not been studied. We identify GDF5 as a novel heparin/ HS-binding protein, and show that HS proteoglycans are vital in localizing GDF5 to the cell surface...
November 29, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27883051/lipid-coated-microbubbles-and-low-intensity-pulsed-ultrasound-enhance-chondrogenesis-of-human-mesenchymal-stem-cells-in-3d-printed-scaffolds
#9
Mitra Aliabouzar, Lijie Grace Zhang, Kausik Sarkar
Lipid-coated microbubbles are used to enhance ultrasound imaging and drug delivery. Here we apply these microbubbles along with low intensity pulsed ultrasound (LIPUS) for the first time to enhance proliferation and chondrogenic differentiation of human mesenchymal stem cells (hMSCs) in a 3D printed poly-(ethylene glycol)-diacrylate (PEG-DA) hydrogel scaffold. The hMSC proliferation increased up to 40% after 5 days of culture in the presence of 0.5% (v/v) microbubbles and LIPUS in contrast to 18% with LIPUS alone...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27882268/long-term-culture-of-intestinal-cell-progenitors-an-overview-of-their-development-application-and-associated-technologies
#10
REVIEW
Andrew J Hollins, Lee Parry
PURPOSE OF REVIEW: Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture. RECENT FINDINGS: Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture. These conditions retain cellular heterogeneity of the normal or tumour tissue, and the cultures have been shown to be genetically stable, such that substantial biobanks are being created from patient derived material...
2016: Current Pathobiology Reports
https://www.readbyqxmd.com/read/27881410/taz-activation-drives-fibroblast-spheroid-growth-expression-of-pro-fibrotic-paracrine-signals-and-context-dependent-ecm-gene-expression
#11
Amy J Jorgenson, Kyoung Moo Choi, Delphine Sicard, Karry M J Smith, Samantha E Hiemer, Xaralabos Varelas, Daniel J Tschumperlin
Recent studies have implicated the Hippo pathway and its transcriptional effectors YAP and TAZ as necessary for fibroblast activation and tissue fibrosis. To test the specific and sufficient roles for TAZ in driving autonomous fibroblast activation, we cultured NIH3T3 fibroblasts expressing a doxycycline-inducible nuclear-localized mutant of TAZ (TAZ4SA) in scaffold-free 3D hanging drop spheroids, or on matrices of specified mechanical rigidity. Control NIH3T3 fibroblasts formed spheroids in hanging drop culture that remained stable and neither increased nor decreased in size significantly over 15 days...
November 23, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27876676/anatomical-region-dependent-enhancement-of-3-dimensional-chondrogenic-differentiation-of-human-mesenchymal-stem-cells-by-soluble-meniscus-extracellular-matrix
#12
Benjamin B Rothrauff, Kazunori Shimomura, Riccardo Gottardi, Peter G Alexander, Rocky S Tuan
: Extracellular matrix (ECM) derived from decellularized tissues has been found to promote tissue neogenesis, most likely mediated by specific biochemical and physical signaling motifs that promote tissue-specific differentiation of progenitor cells. Decellularized ECM has been suggested to be efficacious for the repair of tissue injuries. However, decellularized meniscus contains a dense collagenous structure, which impedes cell seeding and infiltration and is not readily applicable for meniscus repair...
November 19, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27872091/macrophage-secreted-tnf%C3%AE-and-tgf%C3%AE-1-influence-migration-speed-and-persistence-of-cancer-cells-in-3d-tissue-culture-via-independent-pathways
#13
Ran Li, Jess D Hebert, Tara A Lee, Hao Xing, Alexandra Boussommier-Calleja, Richard O Hynes, Douglas A Lauffenburger, Roger D Kamm
The ability of a cancer cell to migrate through the dense extracellular matrix (ECM) within and surrounding the solid tumor is a critical determinant of metastasis. Macrophages enhance invasion and metastasis in the tumor microenvironment but the basis for their effects are not fully understood. Using a microfluidic 3D cell migration assay, we found that the presence of macrophages enhanced the speed and persistence of cancer cell migration through a 3D extracellular matrix in a matrix metalloproteinases (MMP)-dependent fashion...
November 21, 2016: Cancer Research
https://www.readbyqxmd.com/read/27871909/histone-deacetylase-inhibition-enhances-in-vivo-bone-regeneration-induced-by-human-periodontal-ligament-cells
#14
Nam Cong-Nhat Huynh, Vincent Everts, Akira Nifuji, Prasit Pavasant, Ruchanee Salingcarnboriboon Ampornaramveth
: Periodontal ligament cells have the potential to differentiate into bone forming osteoblasts and thus represent a good cellular candidate for bone regeneration. This study aimed to investigate the effect of inhibition of histone deacetylases, using the inhibitor Trichostatin A (TSA), on bone regeneration by human periodontal ligament cells (hPDLCs) in a mouse calvaria bone defect. METHODS: RUNX2 protein and its acetylation was analyzed by immunoprecipitation and western blotting...
November 18, 2016: Bone
https://www.readbyqxmd.com/read/27869294/enhancement-of-human-neural-stem-cell-self-renewal-in-3d-hypoxic-culture
#15
Sasan Sharee Ghourichaee, Elizabeth M Powell, Jennie B Leach
The pathology of neurological disorders is associated with the loss of neuronal and glial cells that results in functional impairments. Human neural stem cells (hNSCs), due to their self-renewing and multipotent characteristics, possess enormous tissue-specific regenerative potential. However, the efficacy of clinical applications is restricted due to the lack of standardized in vitro cell production methods with the capability of generating hNSC populations with well-defined cellular compositions. At any point, a population of hNSCs may include undifferentiated stem cells, intermediate and terminally differentiated progenies, and dead cells...
November 21, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27865067/laminin-modified-and-aligned-phbv-peo-nanofibrous-nerve-conduits-promote-peripheral-nerve-regeneration
#16
Xiao-Feng Zhang, Hai-Xia Liu, Lazarus Santiago Ortiz, Zhong-Dang Xiao, Ning-Ping Huang
Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) has received much attention for its biodegradability and biocompatibility, characteristics which are required in tissue engineering. In this study, polyethylene oxide (PEO)-incorporated PHBV nanofibers with random or aligned orientation were obtained by electrospinning. For further use in vivo, the nanofiber films were made into nerve conduits after treated with NH3 plasma, which could improve the hydrophilicity of inner surfaces of nerve conduits and then facilitate laminin adsorption via electrostatic interaction for promoting cell adhesion and proliferation...
November 12, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27863069/a-straightforward-method-to-produce-decellularized-dermis-based-matrices-for-tumor-cell-cultures
#17
Virginia Brancato, Maurizio Ventre, Giorgia Imparato, Francesco Urciuolo, Concetta Meo, Paolo A Netti
Decellularized matrices are steadily gaining popularity to study the biology of cells and tissues, since they represent a biomimetic environment in which cells can recapitulate certain behaviors that share similarities with those observed in vivo. Basically, biochemistry, microstructure and mechanics of the decellularized matrices are the most valuable properties that differentiate these culturing systems from conventional bidimensional models. Several procedures to decellularize tissues have been proposed so far, with the common aim to preserve the tissue chemical/physical properties of the original tissue...
November 11, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27860335/development-of-a-synthetic-tissue-engineered-3d-printed-bioceramic-based-bone-graft-with-homogenously-distributed-osteoblasts-and-mineralizing-bone-matrix-in-vitro
#18
Doaa Adel-Khattab, Francesca Giacomini, Renate Gildenhaar, Georg Berger, Cynthia Gomes, Ulf Linow, Martin Hardt, Barbara Peleska, Jens Günster, Michael Stiller, Alireza Houshmand, Khaled Abdel Ghaffar, Ahmed Gamal, Mohamed El-Mofty, Christine Knabe
Over the last decade there have been increasing efforts to develop 3D scaffolds for bone tissue engineering from bioactive ceramics with 3D printing emerging as a promising technology. The overall objective of the present study was to generate a tissue engineered synthetic bone graft with homogenously distributed osteoblasts and mineralizing bone matrix in vitro, thereby mimicking the advantageous properties of autogenous bone grafts and facilitating usage for reconstructing segmental discontinuity defects in vivo...
November 15, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27859262/functional-and-prognostic-significance-of-the-genomic-amplification-of-frizzled-receptor-6-fzd6-in-breast-cancer
#19
Gabriele Corda, Gianluca Sala, Rossano Lattanzio, Manuela Iezzi, Michele Sallese, Giorgia Fragassi, Alessia Lamolinara, Hasan Mirza, Daniela Barcaroli, Sibylle Ermler, Elisabete Silva, Hemad Yasaei, Robert F Newbold, Paola Vagnarelli, Marcella Mottolese, Pier Giorgio Natali, Letizia Perracchio, Jelmar Quist, Anita Grigoriadis, Pierfrancesco Marra, Andrew N Tutt, Mauro Piantelli, Stefano Iacobelli, Vincenzo De Laurenzi, Arturo Sala
Frizzled receptors mediate Wnt ligands' signalling, which is crucially involved in regulating tissue development, differentiation and is often deregulated in cancer. In this study we found that the Wnt receptor frizzled 6 (FZD6) is frequently amplified in breast cancer, with an increased incidence in the triple negative breast cancer (TNBC) subtype. Ablation of FZD6 gene expression in mammary cancer cell lines: a) inhibited motility and invasion b) induced a more symmetric shape of organoid 3D cultures c) inhibited bone and liver metastasis in vivo...
November 9, 2016: Journal of Pathology
https://www.readbyqxmd.com/read/27846345/systems-biology-for-organotypic-cell-cultures
#20
Sonia Grego, Edward R Dougherty, Francis J Alexander, Scott S Auerbach, Brian R Berridge, Michael L Bittner, Warren Casey, Philip C Cooley, Ajit Dash, Stephen S Ferguson, Timothy R Fennell, Brian T Hawkins, Anthony J Hickey, Andre Kleensang, Michael N J Liebman, Florian Martin, Elizabeth A Maull, Jason Paragas, Guilin Gary Qiao, Sreenivasa Ramaiahgari, Susan J Sumner, Miyoung Yoon
Translating in vitro biological data into actionable information related to human health holds the potential to improve disease treatment and risk assessment of chemical exposures. While genomics has identified regulatory pathways at the cellular level, translation to the organism level requires a multiscale approach accounting for intra-cellular regulation, inter-cellular interaction, and tissue/organ-level effects. Tissue-level effects can now be probed in vitro thanks to recently developed systems of three-dimensional (3D), multicellular, "organotypic" cell cultures, which mimic functional responses of living tissue...
November 14, 2016: ALTEX
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