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https://www.readbyqxmd.com/read/28342324/short-bursts-of-cyclic-mechanical-compression-modulate-tissue-formation-in-a-3d-hybrid-scaffold
#1
M Brunelli, C M Perrault, D Lacroix
Among the cues affecting cells behaviour, mechanical stimuli are known to have a key role in tissue formation and mineralization of bone cells. While soft scaffolds are better at mimicking the extracellular environment, they cannot withstand the high loads required to be efficient substitutes for bone in vivo. We propose a 3D hybrid scaffold combining the load-bearing capabilities of polycaprolactone (PCL) and the ECM-like chemistry of collagen gel to support the dynamic mechanical differentiation of human embryonic mesodermal progenitor cells (hES-MPs)...
March 18, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28340100/glioblastoma-stem-cell-differentiation-into-endothelial-cells-evidenced-through-live-cell-imaging
#2
Xin Mei, Yin-Sheng Chen, Fu-Rong Chen, Shao-Yan Xi, Zhong-Ping Chen
Background.: Glioblastoma cell-initiated vascularization is an alternative angiogenesis called vasculogenic mimicry. However, current knowledge on the mechanism of de novo vessel formation from glioblastoma stem cells (GSCs) is limited. Methods.: Sixty-four glioblastoma samples from patients and 10 fluorescent glioma xenograft samples were examined by immunofluorescence staining for endothelial marker (CD34 and CD31) and glial cell marker (glial fibrillary acidic protein [GFAP]) expression...
March 8, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28338421/harnessing-the-osteogenicity-of-in-vitro-stem-cell-derived-mineralized-extracellular-matrix-as-3d-biotemplate-to-guide-bone-regeneration
#3
Yoke Chin Chai, Johanna Bolander, Ioannis Papantoniou, Jennifer Patterson, Jef Vleugels, Jan Schrooten, Frank P Luyten
Advanced biomaterials that are capable of guiding robust bone regeneration are highly demanded for translational therapy of bone defects or bone augmentation in clinics. One of the strategic approaches is to produce tissue engineering (TE) constructs that mediate bone regeneration by recapitulating the natural bone formation or healing process. In this study, we aimed at producing devitalized mineralized carriers with augmented bone forming capacity via a modified culture protocol (i.e., culture conditions with high calcium and/or phosphate concentrations) that first promotes cell growth and, subsequently, mineralized extracellular matrix (ECM) deposition by human periosteum-derived osteoprogenitor cells (hPDCs) on additive manufactured three-dimensional (3D) porous titanium (Ti)-based scaffolds...
March 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28337147/3d-proximal-tubule-tissues-recapitulate-key-aspects-of-renal-physiology-to-enable-nephrotoxicity-testing
#4
Shelby M King, J William Higgins, Celina R Nino, Timothy R Smith, Elizabeth H Paffenroth, Casey E Fairbairn, Abigail Docuyanan, Vishal D Shah, Alice E Chen, Sharon C Presnell, Deborah G Nguyen
Due to its exposure to high concentrations of xenobiotics, the kidney proximal tubule is a primary site of nephrotoxicity and resulting attrition in the drug development pipeline. Current pre-clinical methods using 2D cell cultures and animal models are unable to fully recapitulate clinical drug responses due to limited in vitro functional lifespan, or species-specific differences. Using Organovo's proprietary 3D bioprinting platform, we have developed a fully cellular human in vitro model of the proximal tubule interstitial interface comprising renal fibroblasts, endothelial cells, and primary human renal proximal tubule epithelial cells to enable more accurate prediction of tissue-level clinical outcomes...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28332479/3d-tissue-formation-by-stacking-detachable-cell-sheets-formed-on-nanofiber-mesh
#5
Min Sung Kim, Byungjun Lee, Hong Nam Kim, Seokyoung Bang, Hee Seok Yang, Seong Min Kang, Kahp-Yang Suh, Suk-Hee Park, Noo Li Jeon
We present a novel approach for assembling 3D tissue by layer-by-layer stacking of cell sheets formed on aligned nanofiber mesh. A rigid frame was used to repeatedly collect aligned electrospun PCL (polycaprolactone) nanofiber to form a mesh structure with average distance between fibers 6.4 µm. When human umbilical vein endothelial cells (HUVECs), human foreskin dermal fibroblasts, and skeletal muscle cells (C2C12) were cultured on the nanofiber mesh, they formed confluent monolayers and could be handled as continuous cell sheets with areas 3 × 3 cm(2) or larger...
March 23, 2017: Biofabrication
https://www.readbyqxmd.com/read/28325895/simulation-assisted-design-of-microfluidic-sample-traps-for-optimal-trapping-and-culture-of-non-adherent-single-cells-tissues-and-spheroids
#6
Nassim Rousset, Frédéric Monet, Thomas Gervais
This work focuses on modelling design and operation of "microfluidic sample traps" (MSTs). MSTs regroup a widely used class of microdevices that incorporate wells, recesses or chambers adjacent to a channel to individually trap, culture and/or release submicroliter 3D tissue samples ranging from simple cell aggregates and spheroids, to ex vivo tissue samples and other submillimetre-scale tissue models. Numerous MST designs employing various trapping mechanisms have been proposed in the literature, spurring the development of 3D tissue models for drug discovery and personalized medicine...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28325683/biomimetic-scaffolds-with-three-dimensional-undulated-microtopographies
#7
Jonelle Z Yu, Emrullah Korkmaz, Monica I Berg, Philip R LeDuc, O Burak Ozdoganlar
Many human and animal tissues naturally possess three-dimensional (3D) micro-scale geometries enabling certain physiological functions. Absence of these microgeometries in engineered tissues may undermine the effectiveness of corresponding tissue repair and regeneration. This paper introduces a novel approach to create tissue scaffolds with biomimetic 3D undulated microtopographies. The mechanical micromilling technology is used for precise and reproducible fabrication of poly(methyl methacrylate) (PMMA) master molds with 3D undulated microtopographies...
February 14, 2017: Biomaterials
https://www.readbyqxmd.com/read/28322312/imaging-tissue-mimic-with-light-sheet-microscopy-a-comparative-guideline
#8
Jordi Andilla, Raphael Jorand, Omar E Olarte, Alexandre C Dufour, Martine Cazales, Yoann L E Montagner, Romain Ceolato, Nicolas Riviere, Jean-Christophe Olivo-Marin, Pablo Loza-Alvarez, Corinne Lorenzo
Tissue mimics (TMs) on the scale of several hundred microns provide a beneficial cell culture configuration for in vitro engineered tissue and are currently under the spotlight in tissue engineering and regenerative medicine. Due to the cell density and size, TMs are fairly inaccessible to optical observation and imaging within these samples remains challenging. Light Sheet Fluorescence Microscopy (LSFM)- an emerging and attractive technique for 3D optical sectioning of large samples- appears to be a particularly well-suited approach to deal with them...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28321583/microfluidic-encapsulation-of-ovarian-follicles-for-3d-culture
#9
Xiaoming He
The ovarian follicle that contains one single oocyte is the fundamental functional tissue unit of mammalian ovary. Therefore, isolation and in vitro culture of ovarian follicles to obtain fertilizable oocytes are regarded as a promising strategy for women to combat infertility. In this communication, we performed a brief survey of studies on microfluidic encapsulation of ovarian follicles in core-shell hydrogel microcapsules for biomimetic 3D culture. These studies highlighted that recapitulation of the mechanical heterogeneity of the extracellular matrix in ovary is crucial for in vitro culture to develop early pre-antral follicles to the antral stage, and for the release of cumulus-oocyte complex (COC) from antral follicles in vitro...
March 20, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28320945/three-dimensional-culture-system-identifies-a-new-mode-of-cetuximab-resistance-and-disease-relevant-genes-in-colorectal-cancer
#10
Cunxi Li, Bhuminder Singh, Ramona Graves-Deal, Haiting Ma, Alina Starchenko, William H Fry, Yuanyuan Lu, Yang Wang, Galina Bogatcheva, Mohseen P Khan, Ginger L Milne, Shilin Zhao, Gregory Daniel Ayers, Nenggan Li, Huaying Hu, Mary Kay Washington, Timothy J Yeatman, Oliver G McDonald, Qi Liu, Robert J Coffey
We previously reported that single cells from a human colorectal cancer (CRC) cell line (HCA-7) formed either hollow single-layered polarized cysts or solid spiky masses when plated in 3D in type-I collagen. To begin in-depth analyses into whether clonal cysts and spiky masses possessed divergent properties, individual colonies of each morphology were isolated and expanded. The lines thus derived faithfully retained their parental cystic and spiky morphologies and were termed CC (cystic) and SC (spiky), respectively...
March 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28317948/local-remodeling-of-synthetic-extracellular-matrix-microenvironments-by-co-cultured-endometrial-epithelial-and-stromal-cells-enables-long-term-dynamic-physiological-function
#11
Christi D Cook, Abby S Hill, Margaret Guo, Linda Stockdale, Julia P Papps, Keith B Isaacson, Douglas A Lauffenburger, Linda G Griffith
Mucosal barrier tissues, comprising a layer of tightly-bonded epithelial cells in intimate molecular communication with an underlying matrix-rich stroma containing fibroblasts and immune cells, are prominent targets for drugs against infection, chronic inflammation, and other disease processes. Although human in vitro models of such barriers are needed for mechanistic studies and drug development, differences in extracellular matrix (ECM) needs of epithelial and stromal cells hinder efforts to create such models...
March 20, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28314175/choosing-wisely-preclinical-test-models-in-the-era-of-precision-medicine
#12
REVIEW
Konrad Klinghammer, Wolfgang Walther, Jens Hoffmann
Through the introduction of a steadily growing variety of preclinical test models drug development and biomarker research has advanced. Next to classical used 2D cell line cultures, tissue-slice cultures, 3D organoid cell cultures, genetically engineered mouse models, cell line derived mouse models and patient derived xenografts may be selected for a specific question. All models harbor advantages and disadvantages. This review focuses on the available preclinical test models, novel developments such as humanized mice and discusses for which question a particular model should be employed...
March 6, 2017: Cancer Treatment Reviews
https://www.readbyqxmd.com/read/28298644/the-combination-of-three-dimensional-and-rotary-cell-culture-system-promotes-the-proliferation-and-maintains-the-differentiation-potential-of-rat-bmscs
#13
Yilong Tang, Yan Xu, Zhifeng Xiao, Yannan Zhao, Jing Li, Sufang Han, Lei Chen, Bin Dai, Ling Wang, Bing Chen, Hong Wang
Bone marrow mesenchymal stem cells (BMSCs) are a good candidate for tissue engineering and clinical application. One of the challenges in its cell therapy is how to quickly obtain an adequate number of seed cells and meanwhile maintain suitable differentiation potential. In this study we combined three-dimensional (3D) collagen porous scaffolds with rotary cell culture system (RCCS) (RCCS-3D) to create a stereoscopic dynamic environment for the amplification of rat BMSCs in vitro. The results revealed that this RCCS-3D system could enhance BMSCs' proliferation and colony formation, as well as maintain the differentiation potential compared with conventional static two-dimensional (2D) and 3D cell culture conditions...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28298203/methylene-blue-photodynamic-therapy-induces-selective-and-massive-cell-death-in-human-breast-cancer-cells
#14
Ancély F Dos Santos, Letícia F Terra, Rosangela A M Wailemann, Talita C Oliveira, Vinícius de Morais Gomes, Marcela Franco Mineiro, Flávia Carla Meotti, Alexandre Bruni-Cardoso, Maurício S Baptista, Leticia Labriola
BACKGROUND: Breast cancer is the main cause of mortality among women. The disease presents high recurrence mainly due to incomplete efficacy of primary treatment in killing all cancer cells. Photodynamic therapy (PDT), an approach that causes tissue destruction by visible light in the presence of a photosensitizer (Ps) and oxygen, appears as a promising alternative therapy that could be used adjunct to chemotherapy and surgery for curing cancer. However, the efficacy of PDT to treat breast tumours as well as the molecular mechanisms that lead to cell death remain unclear...
March 15, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28297584/improvement-of-in-vitro-three-dimensional-cartilage-regeneration-by-a-novel-hydrostatic-pressure-bioreactor
#15
Jie Chen, Zhaoyuan Yuan, Yu Liu, Rui Zheng, Yao Dai, Ran Tao, Huitang Xia, Hairong Liu, Zhiyong Zhang, Wenjie Zhang, Wei Liu, Yilin Cao, Guangdong Zhou
In vitro three-dimensional (3D) cartilage regeneration is a promising strategy for repair of cartilage defects. However, inferior mechanical strength and tissue homogeneity greatly restricted its clinical translation. Simulation of mechanical stress through a bioreactor is an important approach for improving in vitro cartilage regeneration. The current study developed a hydrostatic pressure (HP) bioreactor based on a novel pressure-transmitting mode achieved by slight deformation of a flexible membrane in a completely sealed stainless steel device...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28297570/human-lung-spheroids-as-in-vitro-niches-of-lung-progenitor-cells-with-distinctive-paracrine-and-plasticity-properties
#16
Isotta Chimenti, Francesca Pagano, Francesco Angelini, Camilla Siciliano, Giorgio Mangino, Vittorio Picchio, Elena De Falco, Mariangela Peruzzi, Roberto Carnevale, Mohsen Ibrahim, Giuseppe Biondi-Zoccai, Elisa Messina, Giacomo Frati
Basic and translational research on lung biology has discovered multiple progenitor cell types, specialized or facultative, responsible for turnover, renewal, and repair. Isolation of populations of resident lung progenitor cells (LPCs) has been described by multiple protocols, and some have been successfully applied to healthy human lung tissue. We aimed at understanding how different cell culture conditions may affect, in vitro, the phenotype of LPCs to create an ideal niche-like microenvironment. The influence of different substrates (i...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28296144/aligned-poly-%C3%AE%C2%B5-caprolactone-nanofibers-guide-the-orientation-and-migration-of-human-pluripotent-stem-cell-derived-neurons-astrocytes-and-oligodendrocyte-precursor-cells-in-vitro
#17
Anu Hyysalo, Mervi Ristola, Tiina Joki, Mari Honkanen, Minnamari Vippola, Susanna Narkilahti
Stem cell transplantations for spinal cord injury (SCI) have been studied extensively for the past decade in order to replace the damaged tissue with human pluripotent stem cell (hPSC)-derived neural cells. Transplanted cells may, however, benefit from supporting and guiding structures or scaffolds in order to remain viable and integrate into the host tissue. Biomaterials can be used as supporting scaffolds, as they mimic the characteristics of the natural cellular environment. In this study, hPSC-derived neurons, astrocytes, and oligodendrocyte precursor cells (OPCs) are cultured on aligned poly(ε-caprolactone) nanofiber platforms, which guide cell orientation to resemble that of spinal cord in vivo...
March 15, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28295953/three-dimensional-carbon-nanotube-scaffolds-for-long-term-maintenance-and-expansion-of-human-mesenchymal-stem-cells
#18
Gaurav Lalwani, Michael D'Agati, Anu Gopalan, Sunny C Patel, Yahfi Talukdar, Balaji Sitharaman
Expansion of mesenchymal stem cells (MSCs) and maintenance of their self-renewal capacity in vitro requires specialized robust cell culture systems. Conventional approaches using animal derived or artificial matrices and a cocktail of growth factors have limitations such as consistency, scalability, pathogenicity and loss of MSC phenotype. Herein, we report the use of all-carbon 3D single- and multiwalled carbon nanotube scaffolds (SWCNTs and MWCNTs) as artificial matrices for long-term maintenance and expansion of human MSCs...
March 10, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28294335/a-novel-scale-down-cell-culture-and-imaging-design-for-the-mechanistic-insight-of-cell-colonisation-within-porous-substrate
#19
C M Gabbott, Z X Zhou, G X Han, T Sun
At the core of translational challenges in tissue engineering is the mechanistic understanding of the underpinning biological processes and the complex relationships among components at different levels, which is a challenging task due to the limitations of current tissue culture and assessment methodologies. Therefore, we proposed a novel scale-down strategy to deconstruct complex biomatrices into elementary building blocks, which were resembled by thin modular substrate and then evaluated separately in miniaturised bioreactors using various conventional microscopes...
March 15, 2017: Journal of Microscopy
https://www.readbyqxmd.com/read/28290667/injectable-polypeptide-thermogel-as-a-tissue-engineering-system-for-hepatogenic-differentiation-of-tonsil-derived-mesenchymal-stem-cells
#20
Ja Hye Hong, Hyun Jung Lee, Byeongmoon Jeong
A poly(ethylene glycol)-b-poly(l-alanine) (PEG-l-PA) hydrogel incorporating tonsil-derived mesenchymal stem cells (TMSCs), tauroursodeoxycholic acid (TUDCA), hepatocyte growth factor (HGF), and fibroblast growth factor 4 (FGF4) was prepared through thermal gelation of an aqueous polymer solution for an injectable tissue engineering application. The thermal gelation accompanied conformational changes of both PA and PEG blocks. The gel modulus at 37 °C was controlled to be 1000 Pa by using a 14.0 wt % aqueous polymer solution...
March 21, 2017: ACS Applied Materials & Interfaces
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