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3D tissue culture

Ai Shima, Yuya Morimoto, H Lee Sweeney, Shoji Takeuchi
This paper describes a method to construct three-dimensional (3D) contractile human skeletal muscle tissues from a cell line. The 3D tissue was fabricated as a fiber-based structure and cultured for two weeks under tension by anchoring its both ends. While myotubes from the immortalized human skeletal myocytes used in this study never contracted in the conventional two-dimensional (2D) monolayer culture, myotubes in the 3D tissue showed spontaneous contraction at a high frequency and also reacted to the electrical stimulation...
June 18, 2018: Experimental Cell Research
Ahmed M Eissa, Flavio S V Barros, Pavle Vrljicak, Jan J Brosens, Neil R Cameron
Novel approaches for culturing primary human cells in vitro are increasingly needed to study cell and tissue physiology and to grow replacement tissue for regenerative medicine. Conventional 2D monolayer cultures of endometrial epithelial and stromal cells fail to replicate the complex 3D architecture of tissue. A fully synthetic scaffold that mimics the microenvironment of the human endometrium can ultimately provide a robust platform for investigating tissue physiology and, hence, take significant steps towards tackling female infertility and IVF failure...
June 21, 2018: Biomacromolecules
Nicola Kavanagh, Fergal J O'Brien, Steve W Kerrigan
Osteomyelitis is an inflammatory bone infection that is caused most commonly by the opportunistic pathogen Staphylococcus aureus. Research into staphylococcal induced bone infection is typically conducted using traditional 2D in vitro culture settings, which is not fully representative of the dynamic in vivo environment. In this study we utilised a collagen glycosaminoglycan scaffold, previously developed for bone tissue engineering, as a representative 3D model of infection. The scaffold resisted degradation and retained its pore structure, which is important for cellular function and survival, when seeded with both cells and bacteria...
2018: PloS One
Irina Zurina, Anastasia Shpichka, Irina Saburina, Nastasia V Kosheleva, Anastasiya Gorkun, Ekaterina A Grebenik, Daria Kuznetsova, Deying Zhang, Yury Rochev, Denis Butnaru, Tatyana Zharikova, Elena Victorovna Istranova, Yuanyuan Zhang, Leonid Prokofievich Istranov, Peter S Timashev
Maintaining the epithelial status of cells in vitro and fabrication of a multilayered epithelial lining is one of the key problems in the therapy using cell technologies. When cultured in a monolayer, epithelial cells change their phenotype from epithelial to epithelial-mesenchymal or mesenchymal that makes it difficult to obtain a sufficient number of cells in a 2D culture and to use them in tissue engineering. Here, using buccal epithelial cells from the oral mucosa, we developed a novel approach to recover and maintain the stable cell phenotype and form a multilayered epithelial lining in vitro via the 2D/3D cell self-assembling...
June 21, 2018: Biomedical Materials
Bogdan Conrad, Li-Hsin Han, Fan Yang
Hydrogels are attractive matrices for cell-based cartilage tissue regeneration given their injectability and ability to fill defects with irregular shapes. However, most hydrogels developed to date often lack cell scale macroporosity, which restrains the encapsulated cells, leading to delayed new extracellular matrix deposition restricted to pericellular regions. Further, tissue engineered cartilage using conventional hydrogels generally suffers from poor mechanical property and fails to restore the load-bearing property of articular cartilage...
June 21, 2018: Tissue Engineering. Part A
Manjulata Singh, Katsuhiko Warita, Tomoko Warita, James R Faeder, Robin E C Lee, Shilpa Sant, Zoltán N Oltvai
Cell-to-cell differences in protein expression in normal tissues and tumors are a common phenomenon, but the underlying principles that govern this heterogeneity are largely unknown. Here, we show that in monolayer cancer cell-line cultures, the expression of the five metabolic enzymes of serine-glycine synthesis (SGS), including its rate-limiting enzyme, phosphoglycerate dehydrogenase (PHGDH), displays stochastic cell-to-cell variation. By contrast, in cancer cell line-derived three-dimensional (3D) microtumors PHGDH expression is restricted to the outermost part of the microtumors' outer proliferative cell layer, while the four other SGS enzymes display near uniform expression throughout the microtumor...
June 20, 2018: Scientific Reports
Beate M Rüger, Tanja Buchacher, Alexander Giurea, Bernd Kubista, Michael B Fischer, Johannes M Breuss
Introduction: New vessel formation requires a continuous and tightly regulated interplay between endothelial cells with cells of the perivascular microenvironment supported by mechanic-physical and chemical cues from the extracellular matrix. Aim: Here we investigated the potential of small fragments of synovial tissue to form de novo vascular structures in the context of inflammation within three dimensional (3D) fibrin-based matrices in vitro , and assessed the contribution of mesenchymal stromal cell (MSC)-immune cell cross-talk to neovascularization considering paracrine signals in a fibrin-based co-culture model...
2018: Frontiers in Physiology
Pujiang Shi, Yang-Hee Kim, Mohamed Mousa, Roxanna Ramnarine Sanchez, Richard O C Oreffo, Jonathan I Dawson
Laponite nanoparticles have attracted attention in the tissue engineering field for their protein interactions, gel-forming properties, and, more recently, osteogenic bioactivity. Despite growing interest in the osteogenic properties of Laponite, the application of Laponite colloidal gels to host the osteogenic differentiation of responsive stem cell populations remains unexplored. Here, the potential to harness the gel-forming properties of Laponite to generate injectable bioactive microenvironments for osteogenesis is demonstrated...
June 17, 2018: Advanced Healthcare Materials
Derek M van Pel, Kaori Harada, Dandan Song, Christian C Naus, Wun Chey Sin
Glioma is a highly aggressive form of brain cancer, with some subtypes having 5-year survival rates of less than 5%. Tumour cell invasion into the surrounding parenchyma seems to be the primary driver of these poor outcomes, as most gliomas recur within 2 cm of the original surgically-resected tumour. Many current approaches to the development of anticancer therapy attempt to target genetic weaknesses in a particular cancer, but may not take into account the microenvironment experienced by a tumour and the patient-specific genetic differences in susceptibility to treatment...
June 16, 2018: Journal of Cell Communication and Signaling
F Azizi, H R Jalil, Z Nasiri, J Moshtaghian, F Esmaeili, A Doostmohammadi, L Shabani, E Ebrahimie
Tissue engineering, as a novel transplantation therapy, aims to create biomaterial scaffolds resembling the extracellular matrix in order to regenerate the damaged tissues. Adding bioactive factors to the scaffold would improve cell-tissue interactions. In this study, the effect of chitosan poly vinyl alcohol nanofibers containing carbon nanotube scaffold with or without active bioglass (BG+ /BG- ), in combination with neonatal rat brain extract (NRBE) on cell viability, proliferation and neural differentiation of P19 embryonic carcinoma (EC) stem cells was investigated...
June 14, 2018: Journal of Tissue Engineering and Regenerative Medicine
Q Q He, A Li, M H Wang, X Gao
Stem cell is critical to regeneration of tissue or organ of human. How to promote repair or regeneration in the tissues/organ using its pluripotency is always an important issue. Lgr5-possitive cell is one type of the stem cell-like cells capable of pluripotent differentiation in various tissues/organs of both humans and mice. Current study showed that single or small amount Lgr5-possitive stem cells can grow and form a plurality of organs in 3D culture system, and some organs can present similar biological and physiological properties with the progenitor they were derived...
June 7, 2018: Zhonghua Er Bi Yan Hou Tou Jing Wai Ke za Zhi, Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Romina Buchs, Bruno Lehner, Phillippe Meuwly, Bruno Schnyder
<i>C. albicans</i> frequently causes recurrent intimal infectious disease (ID). This demands the treatment of multiple phases of the infection. The objective of this study was to uncover the host-pathogen-interaction using 2D epithelium cell-barrier and 3D subepithelium tissue cells of human mucosa. The 2D cell cultures assessed <i>C. albicans</i> adhesion. Addition of the anti-fungal drug Fluconazol did not inhibit the adhesion, despite its pathogen growth inhibition (MIC value 0.08μg/mL)...
June 14, 2018: Tissue Engineering. Part C, Methods
Tzu-En Lin, Stefania Rapino, Hubert H Girault, Andreas Lesch
The technological and experimental progress in electrochemical imaging of biological specimens is discussed with a view on potential applications for skin cancer diagnostics, reproductive medicine and microbial testing. The electrochemical analysis of single cell activity inside cell cultures, 3D cellular aggregates and microtissues is based on the selective detection of electroactive species involved in biological functions. Electrochemical imaging strategies, based on nano/micrometric probes scanning over the sample and sensor array chips, respectively, can be made sensitive and selective without being affected by optical interference as many other microscopy techniques...
May 28, 2018: Chemical Science
XiangGuo Lv, Chao Feng, YiDong Liu, XuFeng Peng, ShiYan Chen, DongDong Xiao, HuaPing Wang, Zhe Li, YueMin Xu, MuJun Lu
Rationale: In urethral tissue engineering, the currently available reconstructive procedures are insufficient due to a lack of appropriate scaffolds that would support the needs of various cell types. To address this problem, we developed a bilayer scaffold comprising a microporous network of silk fibroin (SF) and a nanoporous bacterial cellulose (BC) scaffold and evaluated its feasibility and potential for long-segment urethral regeneration in a dog model. Methods: The freeze-drying and self-assembling method was used to fabricate the bilayer scaffold by stationary cultivation G...
2018: Theranostics
Sandra Laternser, Hansjoerg Keller, Olivier Leupin, Martin Rausch, Ursula Graf-Hausner, Markus Rimann
Two-dimensional (2D) cell cultures do not reflect the in vivo situation, and thus it is important to develop predictive three-dimensional (3D) in vitro models with enhanced reliability and robustness for drug screening applications. Treatments against muscle-related diseases are becoming more prominent due to the growth of the aging population worldwide. In this study, we describe a novel drug screening platform with automated production of 3D musculoskeletal-tendon-like tissues. With 3D bioprinting, alternating layers of photo-polymerized gelatin-methacryloyl-based bioink and cell suspension tissue models were produced in a dumbbell shape onto novel postholder cell culture inserts in 24-well plates...
June 1, 2018: SLAS Technology
Inés Moreno-Jiménez, Stuart A Lanham, Janos M Kanczler, Gry Hulsart-Billstrom, Nicholas D Evans, Richard O C Oreffo
Traditionally used as an angiogenic assay, the chorioallantoic membrane (CAM) assay of the chick embryo offers significant potential as an in vivo model for xenograft organ-culture. Viable human bone can be cultivated on the CAM and increases in bone volume are evident, however, it remains unclear by what mechanism this change occurs and whether this reflects the physiological process of bone remodelling. In this study we tested the hypothesis that CAM-induced bone remodelling is a consequence of host and graft mediated processes...
June 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
Svetlana Shkarina, Roman Shkarin, Venera Weinhardt, Elizaveta Melnik, Gabriele Vacun, Petra Kluger, Kateryna Loza, Matthias Epple, Sergei I Ivlev, Tilo Baumbach, Maria A Surmeneva, Roman A Surmenev
To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 µm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue...
June 11, 2018: Scientific Reports
Atakan Tevlek, Sedat Odabas, Ekin Çelik, Halil Murat Aydin
Cell sheet engineering is an emerging field based on the acquisition of cells together with their extracellular matrix (ECM) and is used not only in vitro but also in regeneration studies of various tissues in the clinic. Within this scope, wide variety of cell types have been investigated in terms of sheet formation and underlying mechanism. MC3T3-E1 is a mouse pre-osteoblast cell line that has greatly attracted researchers' attention for bone tissue engineering (BTE) applications thanks to its high proliferation and differentiation properties...
June 9, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Maryam Rahimi, Amir-Hassan Zarnani, Sahba Mobini, Somaieh Khorasani, Maryam Darzi, Somaieh Kazemnejad
The main purpose of this study was to find effectiveness of 3D silk fibroin scaffold in comparison with co-culturing in presence of native cardiomyocytes on cardiac differentiation propensity of menstural blood(MenSCs)-versus bone marrow-derived stem-cells (BMSCs). We showed that both 3D fibroin scaffold and co-culture system supported efficient cardiomyogenic differentiation of MenSCs and BMSCs, as judged by the expression of cardiac-specific genes and proteins, Connexin-43, Connexin-40, alpha Actinin (ACTN-2), Tropomyosin1 (TPM1) and Cardiac Troponin T (TNNT2)...
June 5, 2018: Biologicals: Journal of the International Association of Biological Standardization
Christine Hellwig, Marta Barenys, Jenny Baumann, Kathrin Gassmann, Laura Casanellas, Gerhard Kauer, Ellen Fritsche
Human neural progenitor cells cultured as neurospheres are a promising tool for developmental neurotoxicity testing in vitro. In order to obtain a human cell-based tissue culture system as close to the organ as possible, it is desirable to improve the spatial organization of the "Neurosphere Assay" and use 3D scaffolds to better mimic the in vivo three dimensional cell microenvironment. For this reason we have established the conditions for short-term culture (up to 6 days) in matrigel or in IKVAV-3 peptide-functionalized hydrogels, and for long-term culture (>25 days) in IKVAV-3 peptide-functionalized hydrogels showing that these conditions support human neural progenitor cells' migration, differentiation to neurons and formation of neuronal networks...
June 5, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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