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FISH testing in bladder cancer

Aaron Mobley, Shizhen Zhang, Jolanta Bondaruk, Yan Wang, Tadeusz Majewski, Nancy P Caraway, Li Huang, Einav Shoshan, Guermarie Velazquez-Torres, Giovanni Nitti, Sangkyou Lee, June Goo Lee, Enrique Fuentes-Mattei, Daniel Willis, Li Zhang, Charles C Guo, Hui Yao, Keith Baggerly, Yair Lotan, Seth P Lerner, Colin Dinney, David McConkey, Menashe Bar-Eli, Bogdan Czerniak
The effects of AURKA overexpression associated with poor clinical outcomes have been attributed to increased cell cycle progression and the development of genomic instability with aneuploidy. We used RNA interference to examine the effects of AURKA overexpression in human bladder cancer cells. Knockdown had minimal effects on cell proliferation but blocked tumor cell invasion. Whole genome mRNA expression profiling identified nicotinamide N-methyltransferase (NNMT) as a downstream target that was repressed by AURKA...
January 19, 2017: Scientific Reports
Ajay Gopalakrishna, Joseph J Fantony, Thomas A Longo, Richmond Owusu, Wen-Chi Foo, Rajesh Dash, Brian T Denton, Brant A Inman
PURPOSE: The aim of this study was to establish the criteria defining an anticipatory positive test for bladder cancer. METHODS: We reviewed all patients at our institution who underwent urine cytology or UroVysion fluorescence in situ hybridization (FISH) and cystoscopy from 2003 to 2012. Test performance and cancer anticipation was assessed using generalized linear mixed models, mixed-effects proportional hazards models, and cumulative incidence curves using tests performed within 30 days of each other as well as within a lag time of 1 year...
January 10, 2017: Annals of Surgical Oncology
Johannes Mischinger, Lutz Philipp Guttenberg, Jörg Hennenlotter, Georgios Gakis, Stefan Aufderklamm, Steffen Rausch, Eva Neumann, Jens Bedke, Stefan Kruck, Christian Schwentner, Arnulf Stenzl, Tilman Todenhöfer
PURPOSE: Urine fluorescence in situ hybridization (FISH) has become a broadly used marker for noninvasive detection of bladder cancer (BC). However, it has been discussed whether the interpretation algorithm proposed by the manufacturer could be improved. Aim of the present study was to compare alternative evaluation strategies of FISH for detection of BC. METHODS: We included 1048 patients suspicious for BC, who underwent urine FISH examination before cystoscopy (diagnostic cohort)...
December 2, 2016: Journal of Cancer Research and Clinical Oncology
Ajay Gopalakrishna, Thomas A Longo, Joseph J Fantony, Richmond Owusu, Wen-Chi Foo, Rajesh Dash, Brant A Inman
BACKGROUND: Spectrum effects refer to the phenomenon that test performance varies across subgroups of a population. When spectrum effects occur during diagnostic testing for cancer, difficult patient misdiagnoses can occur. Our objective was to evaluate the effect of test indication, age, gender, race, and smoking status on the performance characteristics of two commonly used diagnostic tests for bladder cancer, urine cytology and fluorescence in situ hybridization (FISH). METHODS: We assessed all subjects who underwent cystoscopy, cytology, and FISH at our institution from 2003 to 2012...
June 13, 2016: BMC Urology
Tian Zhang, Rengasamy Boominathan, Brad Foulk, Chandra Rao, Gabor Kemeny, John H Strickler, James L Abbruzzese, Michael R Harrison, David S Hsu, Patrick Healy, Jing Li, Cinthia Pi, Katherine M Prendergast, Carey Hobbs, Sarah Gemberling, Daniel J George, Herbert I Hurwitz, Mark Connelly, Mariano A Garcia-Blanco, Andrew J Armstrong
UNLABELLED: Amplification of the MET oncogene is associated with poor prognosis, metastatic dissemination, and drug resistance in many malignancies. We developed a method to capture and characterize circulating tumor cells (CTC) expressing c-MET using a ferromagnetic antibody. Immunofluorescence was used to characterize cells for c-MET, DAPI, and pan-CK, excluding CD45(+) leukocytes. The assay was validated using appropriate cell line controls spiked into peripheral blood collected from healthy volunteers (HV)...
June 2016: Molecular Cancer Research: MCR
Raquel D S Freitas, Kesiane M Costa, Natália F Nicoletti, Luiza W Kist, Maurício R Bogo, Maria M Campos
This study investigated the effects of the long-term dietary fish oil supplementation or the acute administration of the omega-3 fatty acid docosahexaenoic acid (DHA) in the mouse hemorrhagic cystitis (HC) induced by the anticancer drug cyclophosphamide (CYP). HC was induced in mice by a single CYP injection (300mg/kg ip). Animals received four different diets containing 10% and 20% of corn or fish oil, during 21days. Separated groups received DHA by ip (1μmol/kg) or intrathecal (i.t.; 10μg/site) routes, 1h or 15min before CYP...
January 2016: Journal of Nutritional Biochemistry
Emily G Barr Fritcher, Jesse S Voss, Shannon M Brankley, Michael B Campion, Sarah M Jenkins, Matthew E Keeney, Michael R Henry, Sarah M Kerr, Roongruedee Chaiteerakij, Ekaterina V Pestova, Amy C Clayton, Jun Zhang, Lewis R Roberts, Gregory J Gores, Kevin C Halling, Benjamin R Kipp
BACKGROUND & AIMS: Pancreatobiliary cancer is detected by fluorescence in situ hybridization (FISH) of pancreatobiliary brush samples with UroVysion probes, originally designed to detect bladder cancer. We designed a set of new probes to detect pancreatobiliary cancer and compared its performance with that of UroVysion and routine cytology analysis. METHODS: We tested a set of FISH probes on tumor tissues (cholangiocarcinoma or pancreatic carcinoma) and non-tumor tissues from 29 patients...
December 2015: Gastroenterology
Zhiming Ding, Hai-Wei Yang, Tian-Song Xia, Bo Wang, Qiang Ding
The RNA binding motif protein 38 (RBM38, also known as RNPC1) plays a pivotal role in regulating a wide range of biological processes, from cell proliferation and cell cycle arrest to cell myogenic differentiation. It was originally recognized as an oncogene, and was frequently found to be amplified in prostate, ovarian and colorectal cancer, chronic lymphocytic leukemia, colon carcinoma, esophageal cancer, dog lymphomas and breast cancer. In the present study, the complete RNPC1 gene was identified in a number of vertebrate genomes, suggesting that RNPC1 exists in all types of vertebrates, including fish, amphibians, birds and mammals...
August 2015: International Journal of Molecular Medicine
Ashish M Kamat, Daniel L Willis, Rian J Dickstein, Rooselvelt Anderson, Graciela Nogueras-González, Ruth L Katz, Xifeng Wu, H Barton Grossman, Colin P Dinney
OBJECTIVES: To present a molecular definition of bacille Calmette-Guérin (BCG) failure that incorporates fluorescence in situ hybridization (FISH) testing to predict BCG failure before it becomes clinically evident, which can be used to enhance trial designs for patients with non-muscle-invasive bladder cancer. PATIENTS AND METHODS: We used data from 143 patients who were followed prospectively for 2 years during intravesical BCG therapy, during which time FISH assays were collected and correlated to clinical outcomes...
May 2016: BJU International
Tilman Todenhöfer, Jörg Hennenlotter, Philipp Guttenberg, Sarah Mohrhardt, Ursula Kuehs, Michael Esser, Stefan Aufderklamm, Simone Bier, Niklas Harland, Steffen Rausch, Georgios Gakis, Arnulf Stenzl, Christian Schwentner
BACKGROUND: The role of urine markers in the surveillance of patients with non-muscle invasive bladder cancer (NMIBC) is discussed extensively. In case of negative cystoscopy the additional prognostic value of these markers has not been clearly defined yet. The present study is the first systematic approach to directly compare the ability of a urine marker panel to predict the risk of recurrence and progression in bladder cancer (BC) patients with no evidence of relapse during surveillance for NMIBC...
2015: BMC Cancer
Min Yan, Maria Schwaederle, David Arguello, Sherri Z Millis, Zoran Gatalica, Razelle Kurzrock
Human epidermal growth factor receptor 2 (HER2) amplification/overexpression is an effective therapeutic target in breast and gastric cancer. Although HER2 positivity has been reported in other malignancies, previous studies generally focused on one cancer type, making it challenging to compare HER2 positivity across studies/malignancies. Herein, we examined 37,992 patient samples for HER2 expression (+/- amplification) in a single laboratory. All 37,992 patients were tested by immunohistochemistry (IHC); 21,642 of them were also examined for HER2 amplification with either fluorescent in situ hybridization (FISH) (11,670 patients) or chromogenic in situ hybridization (CISH) (9,972 patients); 18,262 patients had tumors other than breast or gastric cancer...
March 2015: Cancer Metastasis Reviews
Tilman Todenhöfer, Jörg Hennenlotter, Michael Esser, Sarah Mohrhardt, Stefan Aufderklamm, Johannes Böttge, Steffen Rausch, Johannes Mischinger, Simone Bier, Georgios Gakis, Ursula Kuehs, Arnulf Stenzl, Christian Schwentner
BACKGROUND: The optimal use of urine markers in the surveillance of non-muscle-invasive bladder cancer (NMIBC) remains unclear. Aim of the present study was to investigate the combined and stepwise use of the four most broadly available urine markers to detect tumor recurrence in patients undergoing surveillance of NMIBC. PATIENTS AND METHODS: 483 patients with history of NMIBC were included. Cytology, UroVysion, fluorescence in situ hybridization (FISH), immunocytology (uCyt+), and NMP22 ELISA were performed before surveillance cystoscopy...
2014: Disease Markers
Casey Seideman, Daniel Canter, Philip Kim, Billy Cordon, Alon Weizer, Irma Oliva, Jianyu Rao, Brant A Inman, Michael Posch, Harry Herr, Yair Lotan
BACKGROUND: The significance of a positive UroVysion FISH assay is uncertain in patients with normal cystoscopy. This multicenter study evaluates the clinical significance of a positive FISH assay in patients with no visible tumor and excluding those with a positive cytology. METHODS: A multi-institutional, retrospective study of patients with a history of urothelial carcinoma of the bladder identified 664 patients with a FISH assay after excluding those with cystoscopic evidence of a tumor and/or positive cytology...
September 2015: World Journal of Urology
Katherine Brown, Richard D Emes, Rachael E Tarlinton
UNLABELLED: Several types of cancer in fish are caused by retroviruses, including those responsible for major outbreaks of disease, such as walleye dermal sarcoma virus and salmon swim bladder sarcoma virus. These viruses form a phylogenetic group often described as the epsilonretrovirus genus. Epsilon-like retroviruses have become endogenous retroviruses (ERVs) on several occasions, integrating into germ line cells to become part of the host genome, and sections of fish and amphibian genomes are derived from epsilon-like retroviruses...
November 2014: Journal of Virology
Mauro Scaravilli, Paola Asero, Teuvo L J Tammela, Tapio Visakorpi, Outi R Saramäki
BACKGROUND: The aim of the study was to characterize a recurrent amplification at chromosomal region 1p21-22 in bladder cancer. METHODS: ArrayCGH (aCGH) was performed to identify DNA copy number variations in 7 clinical samples and 6 bladder cancer cell lines. FISH was used to map the amplicon at 1p21-22 in the cell lines. Gene expression microarrays and qRT-PCR were used to study the expression of putative target genes in the region. RESULTS: aCGH identified an amplification at 1p21-22 in 10/13 (77%) samples...
2014: BMC Research Notes
Christine Mian, Evi Comploj, Elisabeth Resnyak, Emanuela Trenti, Salvatore Palermo, Michele Lodde, Alexander Pycha, Andrea Ambrosini-Spaltro, Christopher B Dechet, Armin Pycha
AIM: To examine the long-term follow-up of patients with that previously underwent risk stratification based on multicolour FISH testing. PATIENTS AND METHODS: On 81 patients with intermediate-risk urothelial carcinoma, a multicolour-FISH was performed. Patients were sub-divided into low- and high-risk groups based on chromosomal patterns. Univariate analysis, using Mantel-Cox log-rank test for disease-free, progression-free survival and overall survival, was employed to determine the prognostic significance of FISH analysis...
June 2014: Anticancer Research
Delia Cavallo, Valentina Casadio, Sara Bravaccini, Sergio Iavicoli, Enrico Pira, Canzio Romano, Anna Maria Fresegna, Raffaele Maiello, Aureliano Ciervo, Giuliana Buresti, Wainer Zoli, Daniele Calistri
The aim of the present study was to identify sensitive and noninvasive biomarkers of early carcinogenic effect at target organ to use in biomonitoring studies of workers at risk for previous occupational exposure to potential carcinogens. Standard urine cytology (Papanicolaou staining test), comet assay, and quantitative telomerase repeat amplification protocol (TRAP) assay were performed in 159 ex-rubber workers employed in tyres production and 97 unexposed subjects. In TRAP positive cases, a second level analysis using FISH (Urovysion) was done...
2014: BioMed Research International
Jordan P Reynolds, Jesse S Voss, Benjamin R Kipp, R Jeffrey Karnes, Aziza Nassar, Amy C Clayton, Michael R Henry, Thomas J Sebo, Jun Zhang, Kevin C Halling
BACKGROUND: The cytologic diagnosis of urothelial carcinoma (UC) of the upper urothelial tract (UT) is challenging. Using the UroVysion probe set adds diagnostic value for the detection of bladder cancer in voided urine. In instrumented UT specimens, the authors encountered positive UT cytology and fluorescence in situ hybridization (FISH) cases that did not demonstrate subsequent UT carcinoma. METHODS: The performance of cytology and FISH in the presence or absence of concomitant bladder cancer within 2 years was compared in 61 patients (112 samples) from 2003 through 2009...
June 2014: Cancer Cytopathology
Marcus Horstmann, Severine Banek, Georgios Gakis, Tilman Todenhöfer, Stefan Aufderklamm, Joerg Hennenlotter, Arnulf Stenzl, Christian Schwentner
OBJECTIVE: To prospectively evaluate the role of fluorescence-guided cystoscopy in a high-risk bladder cancer population undergoing screening based on a multi-marker panel of urine-tests (UroScreen-study). PATIENTS AND METHODS: UroScreen was conducted as a validation study for tumor markers within the frame of a health surveillance program of workers with occupational exposure to aromatic amines. Voluntary annual screens were done in 1,609 men. Cytology, quantitative NMP22® assay, and UroVysion (FISH) were applied to 7091 urine samples...
2014: SpringerPlus
Philip H Kim, Ranjit Sukhu, Billy H Cordon, John P Sfakianos, Daniel D Sjoberg, A Ari Hakimi, Guido Dalbagni, Oscar Lin, Harry W Herr
OBJECTIVE: To assess the ability of reflex UroVysion fluorescence in situ hybridization (FISH) testing to predict recurrence and progression in patients with non-muscle-invasive bladder cancer (NMIBC) with suspicious cytology but negative cystoscopy. PATIENTS AND METHODS: Patients under NMIBC surveillance were followed with office cystoscopy and urinary cytology every 3-6 months. Between March 2007 and February 2012, 500 consecutive patients with suspicious cytology underwent reflex FISH analysis...
September 2014: BJU International
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