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https://www.readbyqxmd.com/read/29779063/development-of-sperm-vitrification-protocols-for-two-endangered-salmonid-species-the-adriatic-grayling-thymallus-thymallus-and-the-marble-trout-salmo-marmoratus
#1
Eszter Kása, Jelena Lujić, Zoran Marinović, Tímea Kollár, Gergely Bernáth, Zoltán Bokor, Béla Urbányi, Kinga Katalin Lefler, Dušan Jesenšek, Ákos Horváth
Vitrification was applied to the sperm of two endangered fish species of Soča River basin in Slovenia, the Adriatic grayling (Thymallus thymallus) and marble trout (Salmo marmoratus) following testing different cooling devices and vitrifying media. Sperm was collected, diluted in species-specific non-activating media containing cryoprotectants, and vitrified by plunging directly into liquid nitrogen without pre-cooling. Progressive motility, curvilinear velocity, and straightness of fresh and vitrified-warmed sperm were evaluated with computer-assisted sperm analysis (CASA)...
May 19, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29778703/sequential-injection-analysis-for-the-determination-of-fluoroquinolone-antibacterial-drug-residues-by-using-eosin-y-as-complexing-agent
#2
Kanokwan Chaiyasing, Boonsom Liawruangrath, Surapol Natakankitkul, Sakchai Satienperakul, Narabhats Rannurags, Poachanee Norfun, Saisunee Liawruangrath
A sequential injection analysis (SIA) method was developed for the rapid and sensitive determination of fluoroquinolone residues, including norfloxacin, ciprofloxacin and enrofloxacin, in fish samples. The method is based on the reaction between fluoroquinolone drug and eosin Y in Britton-Robinson buffer (pH 2.0), forming pink colored complexs with maximum absorptions at 522, 525 and 527 nm for norfloxacin, ciprofloxacin and enrofloxacin, respectively. Linearity ranges were found to be 0.05-10.0 mg L-1 (r2  = 0...
May 9, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29778682/temperature-and-hydrologic-alteration-predict-the-spread-of-invasive-largemouth-bass-micropterus-salmoides
#3
Mi-Jung Bae, Christina A Murphy, Emili García-Berthou
The successful establishment of an aquatic invasive alien species can be mediated by a suite of environmental factors, including climate and anthropogenic disturbance. Dams and reservoirs are thought to promote freshwater fish invasion success through hydrological alterations but the evidence for their role in the global invasion of Largemouth Bass (Micropterus salmoides) on a landscape scale is limited. Here, we examine the distribution of Largemouth Bass, one of the most widely introduced fish in the world, from the Iberian Peninsula using species distribution models (SDMs), including an ensemble forecast...
May 17, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29778226/imbalanced-nutrient-regimes-increase-prymnesium-parvum-resilience-to-herbicide-exposure
#4
Stacie L Flood, JoAnn M Burkholder
The toxigenic haptophyte Prymnesium parvum is a mixotrophic phytoplankter with an extensive historic record of forming nearly monospecific, high-biomass, ecosystem-disrupting blooms, and it has been responsible for major fish kills in brackish waters and aquaculture facilities in many regions of the world. Little is known about how this species responds to commonly occurring environmental contaminants, or how nutrient (nitrogen, phosphorus) pollution may interact with environmentally relevant pesticide exposures to affect this harmful algal species...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29778221/morphology-and-toxicity-of-pseudo-nitzschia-species-in-the-northern-benguela-upwelling-system
#5
Deon C Louw, Gregory J Doucette, Nina Lundholm
The Benguela upwelling system, considered the world's most productive marine ecosystem, has a long record of potentially toxic diatoms belonging to the genus Pseudo-nitzschia. Species of Pseudo-nitzschia were reported as early as 1936 from the northern Benguela upwelling system (nBUS). For the current study, long-term phytoplankton monitoring data (2004-2011) for the Namibian coast were analysed to examine inshore and offshore temporal distribution of Pseudo-nitzschia species, their diversity and ultrastructure...
May 2018: Harmful Algae
https://www.readbyqxmd.com/read/29778079/advances-in-understanding-the-response-of-fish-to-linear-alcohols-in-the-environment
#6
Scott E Belanger, Jane M Rawlings, Ricky Stackhouse
Short to long chain alcohols have a range of ecotoxicity to aquatic life driven by hydrophobic interactions with biological membranes. Carbon chain length and octanol:water partitioning coefficients are surrogates for hydrophobicity and strongly relate to aquatic toxicity. In these investigations, the toxicity of ethanol to 1-n-dodecanol to juvenile fish in standard acute toxicity tests is reviewed. Toxicity tests employing fish embryos (zebrafish Danio rerio and fathead minnow Pimephales promelas) in the Fish Embryo Test (OECD 236) format were conducted from C2 to C10 to compare against standard juvenile fish toxicity...
April 25, 2018: Chemosphere
https://www.readbyqxmd.com/read/29778078/an-effective-and-low-cost-carbon-based-clean-up-method-for-pcdd-fs-and-pcbs-analysis-in-food
#7
Kleopatra Kedikoglou, Danae Costopoulou, Irene Vassiliadou, Evangelos Bakeas, Leondios Leondiadis
Sample preparation is of critical importance in dioxin analysis of food and feed samples. It is a complex procedure that includes lipid extraction followed by the application of chromatographic separation techniques, aiming in removing undesirable interferences from the matrix. The separation of polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) from polychlorinated biphenyls (PCBs) is achieved by carbon-based materials which should have high fat capacity in order to be suitable for lipid-containing matrices...
May 7, 2018: Chemosphere
https://www.readbyqxmd.com/read/29777853/investigation-of-the-potential-endocrine-effect-of-nitrate-in-zebrafish-danio-rerio-and-brown-trout-salmo-trutta
#8
Poul Bjerregaard, Karin Lund Kinnberg, Maria Pedersen Mose, Henrik Holbech
Nitrate has the potential to affect steroid production. Nitrate concentrations in streams in agricultural areas may exceed concentrations showing effects in laboratory studies. The effects of nitrate and/or nitrite on endocrine relevant endpoints were tested in zebrafish and brown trout. Zebrafish were exposed in two experiments to nitrate (8.8 to 89 mg NO3 - /L) and nitrite (3.6 to 19 mg NO2 - /L) during the period of sexual differentiation and sex ratios were determined. Vitellogenin concentrations were determined in the second experiment...
May 16, 2018: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/29777392/determining-representative-ranges-of-point-sensors-in-distributed-networks
#9
John K Horne, Dale A Jacques
Distributed networks of stationary instruments provide high temporal scope (i.e., range/resolution) observations but are spatially limited as a set of point measurements. Measurement similarity between points typically decays with distance, which is used to set interpolation distances. The importance of analyzing spatiotemporal data at equivalent spatial and temporal scales has been identified but no standard procedure is used to interpolate space using temporally-indexed observations. Using concurrent mobile and stationary active acoustic, fish density data from a tidal energy site in Puget Sound, WA, USA, six methods are compared to estimate the range at which stationary measurements can be spatially interpolated...
May 18, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29775944/alterations-of-cytochrome-p450-and-the-occurrence-of-persistent-organic-pollutants-in-tilapia-caged-in-the-reservoirs-of-the-igua%C3%A3-u-river
#10
F Y Yamamoto, G D Diamante, M S Santana, D R Santos, R Bombardeli, C C Martins, C A Oliveira Ribeiro, D Schlenk
Environmental chemicals originating from human activities, such as persistent organic pollutants (POPs), may interfere with the endocrine system of aquatic organisms. The effect of these chemicals on biota and human populations is of high public concern but remains poorly understood, especially in aquatic environments of South America. The aim of this study was to investigate the bioavailability of POPs and the related effects in caged male tilapia (Oreochromis niloticus) in four cascading reservoirs of the Iguaçu River, Southern Brazil...
May 15, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29775741/replacement-of-fishmeal-by-spirulina-arthrospira-platensis-affects-growth-immune-related-gene-expression-in-gibel-carp-carassius-auratus-gibelio-var-cas-iii-and-its-challenge-against-aeromonas-hydrophila-infection
#11
Shenping Cao, Peiyu Zhang, Tao Zou, Shuzhan Fei, Dong Han, Junyan Jin, Haokun Liu, Yunxia Yang, Xiaoming Zhu, Shouqi Xie
The present study examined the effect of dietary spirulina, Arthrospira platensis on growth performance, blood physiological indices, immune-related gene expressions and resistance of juvenile gibel carp against Aeromonas hydrophila infection. Four isonitrogenous (360 g kg-1 ) and isolipidic (90 g kg-1 ) diets were formulated with containing different levels of spirulina powder of 0 g (SP0, the control diet), 3.38 g (SP3.38), 6.76 g (SP6.76) and 13.52 g (SP13.52) per 100 g diet to replace 0%, 25%, 50% and 100% of fishmeal protein, respectively...
May 15, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29773843/loss-of-live-coral-compromises-predator-avoidance-behaviour-in-coral-reef-damselfish
#12
Lisa Boström-Einarsson, Mary C Bonin, Philip L Munday, Geoffrey P Jones
Tropical reefs have experienced an unprecedented loss of live coral in the past few decades and the biodiversity of coral-dependent species is under threat. Many reef fish species decline in abundance as coral cover is lost, yet the mechanisms responsible for these losses are largely unknown. A commonly hypothesised cause of fish decline is the loss of shelter space between branches as dead corals become overgrown by algae. Here we tested this hypothesis by quantifying changes in predator-avoidance behaviour of a common damselfish, Pomacentrus moluccensis, before and after the death of their coral colony...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29773426/stem-cell-transcription-factor-sox2-in-synovial-sarcoma-and-other-soft-tissue-tumors
#13
Heba Zayed, Iver Petersen
BACKGROUND: SOX2 has gained considerable interest as a pluripotency inducing gene. Co-transfection of SOX2 together with NANOG, KLF4 and c-MYC into adult fibroblasts was able to generate pluripotent stem cells. SOX2 has been reported to be expressed in synovial sarcoma, a tumor being characterized by the SS18-SSX gene fusion forming part of the SWI/SNF chromatin remodeling complex that affects histone methylation. The role of SOX2 in this tumor type as well as other soft tissue tumor entities however is still poorly characterized...
May 4, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29770570/an-adaptive-environmental-effects-monitoring-framework-for-assessing-the-influences-of-liquid-effluents-on-benthos-water-and-sediments-in-aquatic-receiving-environments
#14
REVIEW
Keith M Somers, Bruce W Kilgour, Kelly R Munkittrick, Tim J Arciszewski
Environmental effects monitoring (EEM) has been traditionally used to evaluate the effects of existing facilities discharging liquid effluents into natural receiving waters in Canada. EEM also has the potential to provide feedback to an ongoing project in an adaptive management context, and can inform the design of future projects. EEM, consequently, can and should also be used to test the predictions of effects related to new projects. Despite EEM's potential for widespread applicability, challenges related to the effective implementation of EEM include the use of appropriate study designs, as well as to the adoption of tiers for increasing or decreasing monitoring intensity...
May 16, 2018: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/29770449/effects-of-a-nanoemulsion-with-copaifera-officinalis-oleoresin-against-monogenean-parasites-of-colossoma-macropomum-a-neotropical-serrasalmidae
#15
D S S Valentim, J L Duarte, A E M F M Oliveira, R A S Cruz, J C T Carvalho, C Solans, C P Fernandes, M Tavares-Dias
Monogeneans are ectoparasites that may cause losses in production and productivity in the aquaculture of Colossoma macropomum. Chemotherapeutics used in aquaculture usually have major adverse effects on fish; hence, the use of essential oils has been considered advantageous, but these are not soluble in water. Thus, the use of nanostructures to enhance water solubility of compounds and improve bioactivity may be very promising. This study investigated the antiparasitic activity of nanoemulsion prepared with Copaifera officinalis oleoresin (50, 100, 150, 200 and 300 mg/L), against monogenean parasites from the gills of C...
May 16, 2018: Journal of Fish Diseases
https://www.readbyqxmd.com/read/29769272/original-ligand-for-lt%C3%AE-r-is-light-insight-into-evolution-of-the-lt-lt%C3%AE-r-system
#16
Tomoki Maeda, Hiroaki Suetake, Tomoyuki Odaka, Toshiaki Miyadai
The lymphotoxin (LT)/LTβ receptor (LTβR) axis is crucial for the regulation of immune responses and development of lymphoid tissues in mammals. Despite the importance of this pathway, the existence and function of LT and LTβR remain obscure for nonmammalian species. In this study, we report a nonmammalian LTβR and its ligand. We demonstrate that TNF-New (TNFN), which has been considered orthologous to mammalian LT, was expressed on the cell surface as a homomer in vitro. This different protein structure indicates that TNFN is not orthologous to mammalian LTα and LTβ...
May 16, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29768738/unlocking-biodiversity-and-conservation-studies-in-high-diversity-environments-using-environmental-dna-edna-a-test-with-guianese-freshwater-fishes
#17
Kévin Cilleros, Alice Valentini, Luc Allard, Tony Dejean, Roselyne Etienne, Gaël Grenouillet, Amaia Iribar, Pierre Taberlet, Régis Vigouroux, Sébastien Brosse
Determining the species compositions of local assemblages is a prerequisite to understanding how anthropogenic disturbances affect biodiversity. However, biodiversity measurements often remain incomplete due to the limited efficiency of sampling methods. This is particularly true in freshwater tropical environments that host rich fish assemblages, for which assessments are uncertain and often rely on destructive methods. Developing an efficient and non-destructive method to assess biodiversity in tropical freshwaters is highly important...
May 16, 2018: Molecular Ecology Resources
https://www.readbyqxmd.com/read/29767801/reliability-of-in-vitro-methods-used-to-measure-intrinsic-clearance-of-hydrophobic-organic-chemicals-by-rainbow-trout-results-of-an-international-ring-trial
#18
John Nichols, Kellie Fay, Mary Jo Bernhard, Ina Bischof, John Davis, Marlies Halder, Jing Hu, Karla Johanning, Heike Laue, Diane Nabb, Christian Schlechtriem, Helmut Segner, Joe Swintek, John Weeks, Michelle Embry
In vitro assays are widely employed to obtain intrinsic clearance estimates used in toxicokinetic modeling efforts. However, the reliability of these methods is seldom reported. Here we describe the results of an international ring trial designed to evaluate two in vitro assays used to measure intrinsic clearance in rainbow trout. An important application of these assays is to predict the effect of biotransformation on chemical bioaccumulation. Six laboratories performed substrate depletion experiments with cyclohexyl salicylate, fenthion, 4-n-nonylphenol, deltamethrin, methoxychlor, and pyrene using cryopreserved hepatocytes and liver S9 fractions from trout...
May 14, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29767108/optimizing-protein-and-lipid-levels-in-practical-diet-for-juvenile-northern-snakehead-fish-channa-argus
#19
Gladstone Sagada, Jianming Chen, Binqian Shen, Aixia Huang, Lihui Sun, Jianhu Jiang, Chunhua Jin
A 3 × 3 factorial feeding trial was conducted to evaluate the production response of juvenile northern snakehead fish ( Channa argus ). Nine diets containing 3 protein levels (45%, 48% and 51%) and 3 lipid levels (9%, 12% and 15%) were formulated and fed to triplicate groups of juvenile northern snakehead (15.78 ± 0.09 g/fish) for 8 weeks. The formulated diets were named as P45L9, P45L12, P45L15, P48L9, P48L12, P48L15, P51L9, P51L12 and P51L15 (P-Protein, L-Lipid), respectively. Fish fed diets with the lowest protein and lipid combination (P45L9) had the lowest growth performance...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29767074/occurrence-of-clostridium-perfringens-contamination-in-poultry-feed-ingredients-isolation-identification-and-its-antibiotic-sensitivity-pattern
#20
Shanmugasundaram Udhayavel, Gopalakrishnamurthy Thippichettypalayam Ramasamy, Vasudevan Gowthaman, Shanmugasamy Malmarugan, Kandasamy Senthilvel
This work has been undertaken to study the occurrence of Clostridium perfringens contamination in the poultry feed ingredients and find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs. Two hundred and ninety-eight poultry feed ingredient samples received at Poultry Disease Diagnosis and Surveillance Laboratory, Namakkal, Tamil Nadu in South India were screened for the presence of C. perfringens . The organisms were isolated in Perfringens agar under anaerobic condition and subjected to standard biochemical tests for confirmation...
September 2017: Animal nutrition
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