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https://www.readbyqxmd.com/read/28231386/evaluating-the-effectiveness-of-rna-in-situ-hybridization-for-detecting-lung-adenocarcinoma-with-anaplastic-lymphoma-kinase-rearrangement
#1
Naoki Nakajima, Akihiko Yoshizawa, Kyoko Kondo, Mariyo Rokutan-Kurata, Masahiro Hirata, Ayako Furuhata, Shinji Sumiyoshi, Makoto Sonobe, Toshi Menju, Masanobu Momose, Masakazu Fujimoto, Hiroshi Date, Hironori Haga
AIMS: An easy and rapid assay for detecting mRNA in formalin-fixed paraffin-embedded samples (RNA-in situ hybridization; RNA-ISH) has been reported recently. We investigated the diagnostic accuracy of RNA-ISH for detecting lung adenocarcinoma (LA) with anaplastic lymphoma kinase (ALK) gene rearrangement. METHODS AND RESULTS: We tested ALK RNA-ISH on 11 resected LAs for which ALK fusion was confirmed by immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH)...
February 23, 2017: Histopathology
https://www.readbyqxmd.com/read/28231234/notes-from-the-field-francisella-tularensis-type-b-infection-from-a-fish-hook-injury-minnesota-2016
#2
Tory Whitten, Jenna Bjork, Dave Neitzel, Kirk Smith, Maureen Sullivan, Joni Scheftel
On June 27, 2016, the Minnesota Department of Health (MDH) Public Health Laboratory (PHL) was notified of a suspected Francisella tularensis isolate cultured at a hospital laboratory. The isolate was confirmed as F. tularensis type B at MDH PHL by reverse transcription-polymerase chain reaction, culture, and direct fluorescent antibody testing. Francisella tularensis subspecies tularensis (type A) and holarctica (type B) bacteria are the causative agents of tularemia.
February 24, 2017: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/28229024/modafinil-decreases-anxiety-like-behaviour-in-zebrafish
#3
Adrian Johnson, Trevor James Hamilton
Modafinil (2-((diphenylmethyl)sulfinyl)acetamide), a selective dopamine and norepinephrine transporter inhibitor, is most commonly prescribed for narcolepsy but has gained recent interest for treating a variety of disorders. Zebrafish (Danio rerio) are becoming a model of choice for pharmacological and behavioural research. To investigate the behavioural effects of modafinil on anxiety, we administered doses of 0, 2, 20, and 200 mg/L for 30 minutes then tested zebrafish in the novel approach test. In this test, the fish was placed into a circular arena with a novel object in the center and motion-tracking software was used to quantify the time the fish spent in the outer area of the arena (thigmotaxis zone), middle third of the arena (transition zone) and center of the arena, as well as total distance traveled, immobility and meandering...
2017: PeerJ
https://www.readbyqxmd.com/read/28226324/a-survey-of-self-reported-food-allergy-and-food-related-anaphylaxis-among-young-adult-students-at-kuwait-university-kuwait
#4
Fatima Ali
OBJECTIVES: To conduct a survey on self-reported food allergy to milk, egg, peanuts, tree nuts, fish, shellfish, wheat and sesame as well as reported food-related anaphylaxis and epinephrine prescription practices among Kuwaiti students attending Kuwait University. SUBJECTS AND METHODS: One thousand (1,000) questionnaires that included data on age, gender, presence of food allergies and associated details were distributed. All participants reporting an allergy were categorized as having a perceived food allergy...
February 22, 2017: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/28223733/impact-of-humic-acids-on-the-colonic-microbiome-in-healthy-volunteers
#5
Alexander Swidsinski, Yvonne Dörffel, Vera Loening-Baucke, Christoph Gille, Anne Reißhauer, Onder Göktas, Monika Krüger, Jürgen Neuhaus, Wieland Schrödl
AIM: To test the effects of humic acids on innate microbial communities of the colon. METHODS: We followed the effects of oral supplementation with humic acids (Activomin(®)) on concentrations and composition of colonic microbiome in 14 healthy volunteers for 45 d. 3 × 800 mg Activomin(®) were taken orally for 10 d followed by 3 × 400 mg for 35 d. Colonic microbiota were investigated using multicolor fluorescence in situ hybridization (FISH) of Carnoy fixated and paraffin embedded stool cylinders...
February 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28223491/oogenesis-and-reproductive-investment-of-atlantic-herring-are-functions-of-not-only-present-but-long-ago-environmental-influences-as-well
#6
Thassya C Dos Santos Schmidt, Aril Slotte, James Kennedy, Svein Sundby, Arne Johannessen, Gudmundur J Óskarsson, Yutaka Kurita, Nils C Stenseth, Olav Sigurd Kjesbu
Following general life history theory, immediate reproductive investment (egg mass × fecundity/body mass) in oviparous teleosts is a consequence of both present and past environmental influences. This clarification questions the frequent use of season-independent (general) fecundity formulas in marine fish recruitment studies based on body metrics only. Here we test the underlying assumption of no lag effect on gametogenesis in the planktivorous, determinate-fecundity Atlantic herring (Clupea harengus) displaying large plasticity in egg mass and fecundity, examining Norwegian summer-autumn spawning herring (NASH), North Sea autumn-spawning herring (NSAH), and Norwegian spring-spawning herring (NSSH)...
February 21, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28223437/long-term-administration-of-eicosapentaenoic-acid-improves-post-myocardial-infarction-cardiac-remodeling-in-mice-by-regulating-macrophage-polarization
#7
Masayuki Takamura, Keisuke Kurokawa, Hiroshi Ootsuji, Oto Inoue, Hikari Okada, Ayano Nomura, Shuichi Kaneko, Soichiro Usui
BACKGROUND: Consumption of n-3 fatty acids reduces the incidence of cardiovascular mortality in populations that consume diets rich in fish oil. Eicosapentaenoic acid (EPA) is an n-3 fatty acid known to reduce the frequency of nonfatal coronary events; however, the frequency of mortality after myocardial infarction (MI) is not reduced. The aims of this study were to determine whether long-term administration of EPA regulated cardiac remodeling after MI and to elucidate the underlying therapeutic mechanisms of EPA...
February 21, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28223237/consumer-preferences-for-sustainable-aquaculture-products-evidence-from-in-depth-interviews-think-aloud-protocols-and-choice-experiments
#8
Antje Risius, Meike Janssen, Ulrich Hamm
Fish from aquaculture is becoming more important for human consumption. Sustainable aquaculture procedures were developed as an alternative to overcome the negative environmental impacts of conventional aquaculture procedures and wild fisheries. The objective of this contribution is to determine what consumers expect from sustainable aquaculture and whether they prefer sustainable aquaculture products. A combination of qualitative research methods, with think aloud protocols and in-depth interviews, as well as quantitative methods, using choice experiments and face-to-face interviews, was applied...
February 18, 2017: Appetite
https://www.readbyqxmd.com/read/28223206/accumulation-of-heavy-metals-in-people-on-a-gluten-free-diet
#9
Stephanie L Raehsler, Rok Seon Choung, Eric V Marietta, Joseph A Murray
BACKGROUND & AIMS: Specific foods such as fish and rice have high concentrations of metals such as arsenic, mercury, lead, cadmium, and cobalt. Many gluten-free diets (GFDs) include these foods, so we evaluated whether a GFD was associated with increased metal bioaccumulation. METHODS: We performed a population-based cross-sectional study using data collected from the National Health and Nutrition Examination Survey (NHANES), from 2009 through 2012, collecting information on the diagnosis of celiac disease and adherence to a GFD...
February 18, 2017: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28223149/long-term-behavioral-change-as-a-result-of-acute-ethanol-exposure-in-zebrafish-evidence-for-a-role-for-sonic-hedgehog-but-not-retinoic-acid-signaling
#10
Derek F Burton, Chengjin Zhang, Oswald Boa-Amponsem, Shanta Mackinnon, Gregory J Cole
BACKGROUND: Developmental exposure to ethanol is recognized to produce long-term neurobehavioral impairment in multiple animal models. However, the molecular mechanisms underlying these deficits remain poorly understood. The present study was undertaken to ascertain whether two well-characterized targets of prenatal alcohol exposure, sonic hedgehog (Shh) and retinoic acid (RA), that induce the hallmark morphological phenotypes of fetal alcohol spectrum disorders (FASD), are involved in the generation of behavioral alterations as a result of alcohol exposure...
February 20, 2017: Neurotoxicology and Teratology
https://www.readbyqxmd.com/read/28222923/consumption-of-aquaculture-waste-affects-the-fatty-acid-metabolism-of-a-benthic-invertebrate
#11
Camille A White, Raymond J Bannister, Symon A Dworjanyn, Vivian Husa, Peter D Nichols, Tina Kutti, Tim Dempster
Trophic subsidies can drive widespread ecological change, thus knowledge of how keystone species respond to subsidies is important. Aquaculture of large carnivorous fish generates substantial waste as faeces and lost feed, providing a food source to mobile benthic invertebrates. We used a controlled feeding study combined with a field survey to better understand the interaction between salmon aquaculture and the sea urchin, Echinus acutus, a dominant mobile invertebrate in Norwegian fjords. We tested if diets affected urchin fatty acid composition by feeding them one of three diet treatments ("aquafeed", "composite" and "natural") for 10weeks...
February 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28221781/the-relationship-between-organic-loading-and-effects-on-fish-reproduction-for-pulp-mill-effluents-across-canada
#12
Pierre H Martel, Brian I O'Connor, Tibor G Kovacs, Michael R Van Den Heuvel, Joanne L Parrott, Mark E McMaster, Deborah L MacLatchy, Glen J Van Der Kraak, L Mark Hewitt
This study builds upon the work of a multiagency consortium tasked with determining cost effective solutions for the effects of pulp mill effluents on fish reproduction. A laboratory fathead minnow egg production test and chemical characterization tools were used to benchmark eighty-one effluents from twenty mills across Canada, representing the major pulping, bleaching and effluent treatment technologies. For Kraft and mechanical pulp mills, effluents containing less than 20 mg/L BOD5 were found to have the greatest probability of having no effects...
February 21, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28220850/comparing-the-survival-rate-of-juvenile-chinook-salmon-migrating-through-hydropower-systems-using-injectable-and-surgical-acoustic-transmitters
#13
Z D Deng, J J Martinez, H Li, R A Harnish, C M Woodley, J A Hughes, X Li, T Fu, J Lu, G A McMichael, M A Weiland, M B Eppard, J R Skalski, R L Townsend
Acoustic telemetry is one of the primary technologies for studying the behavior and survival of fishes throughout the world. The size and performance of the transmitter are key limiting factors. The newly developed injectable transmitter is the first acoustic transmitter that can be implanted via injection instead of surgery. A two-part field study was conducted to evaluate the performance of the injectable transmitter and its effect on the survival of implanted fish. The injectable transmitter performed well and similarly to the proceeding generation of commercially-available JSATS transmitters tested concurrently...
February 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28220339/triphenyltin-hydroxide-induces-changes-in-the-oxidative-stress-parameters-of-fish
#14
Bárbara Clasen, Alexssandro G Becker, Thais Lópes, Camila R Murussi, Fabiane G Antes, Roberta C Horn, Érico M M Flores, Bernardo Baldisserotto, Valderi L Dressler, Vania L Loro
Among all organotin compounds , triphenyltin hydroxide (TPhTH) is widely used as fungicide and moluscicide in Brazil. However, the effects of TPhTH on the biochemical parameters of non-target organisms, such as fish, are little known. The aim of the present study is to assess the possible toxic effects of different concentrations of waterborne TPhTH on silver catfish belonging to species Rhamdia quelen. The fish were exposed to two different concentrations of TPhTH (1.08 and 1.70 µg/L as Sn) for 15 days and then compared to the control group (triplicate, n = 3)...
February 20, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28220284/untangling-convoluted-taxonomy-of-chambriella-rego-chubb-pavanelli-1999-cestoda-proteocephalidae-with-erection-of-riggenbachiella-n-g-and-the-description-of-a-new-species-from-pimelodid-catfishes-in-the-neotropical-region
#15
Philippe Vieira Alves, Alain de Chambrier, José Luis Luque, Tomáš Scholz
As part of a complex revision of proteocephalid cestodes parasitic in freshwater bony fishes of the Neotropical Region, the genus Chambriella Rego, Chubb & Pavanelli, 1999 is redefined based on detailed examination of type-specimens and newly collected material of both nominal species of the genus. This examination revealed that the type-species C. agostinhoi (Pavanelli & Santos, 1992) from Zungaro jahu (Ihering) (type-host) and Z. zungaro (Humboldt) is indistinguishable from Lenhataenia megacephala (Woodland, 1934) from Sorubimichthys planiceps (Spix & Agassiz) (all hosts Siluriformes: Pimelodidae), the type- and only species of the genus...
February 20, 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28219247/addressing-analytical-challenges-of-the-environmental-monitoring-for-the-water-framework-directive-erm-ce100-a-new-biota-certified-reference-material
#16
Ioannis Dosis, Marina Ricci, Laszlo Majoros, Roberto Lava, Håkan Emteborg, Andrea Held, Hendrik Emons
In the context of supporting the EU Member States in the implementation of the EU Water Framework Directive (WFD), a project for the production of a fish reference material (ERM-CE100) certified for its content of the two priority substances hexachlorobenzene (HCB) and hexachlorobutadiene (HCBD) was carried out at the Joint Research Centre (JRC) of the European Commission. The starting material was naturally contaminated Wels catfish (Silurus glanis), caught in the Ebro River (Spain). A novel approach for the processing of the fish was tested that resulted in a homogeneous and stable reference material in the form of a wet paste...
February 21, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28219220/-clinical-significance-of-expressions-of-evi1-and-bre-genes-in-47-acute-leukemia-patients-with-mll-rearrangement
#17
Y L Gong, J R Zhang, J Zhang, S X Bai, J N Cen, H J Shen, H Y Chou, S N Chen, J L Pan
Objective: To investigate the overexpression frequencies of BRE and EVI1, the correlation between BRE and EVI1 expressions and their possible clinical implications in 11q23/MLL rearrangement acute leukemia. Methods: Cytogenetic examination of bone marrow cells was performed by short-term culture method. R-banding technique was used for karyotype analysis. 47 patients were detected by interphase fluorescence in situ hybridization (FISH) with dual-color break apart MLL probe. The expressions of EVI1 and BRE genes were detected by real time quantitative reverse transcription polymerase chain reaction (RQ-PCR) ...
January 14, 2017: Zhonghua Xue Ye Xue za Zhi, Zhonghua Xueyexue Zazhi
https://www.readbyqxmd.com/read/28216433/lifelong-memory-responses-perpetuate-humoral-th2-immunity-and-anaphylaxis-in-food-allergy
#18
Rodrigo Jiménez-Saiz, Derek K Chu, Talveer S Mandur, Tina D Walker, Melissa E Gordon, Roopali Chaudhary, Joshua Koenig, Sarah Saliba, Heather J Galipeau, Adam Utley, Irah L King, Kelvin Lee, Rachel Ettinger, Susan Waserman, Roland Kolbeck, Manel Jordana
BACKGROUND: A number of food allergies (e.g. fish, shellfish, nuts) are lifelong, without any disease-transforming therapies and unclear in their underlying immunology. Clinical manifestations of food allergy are largely mediated by IgE. Although persistent IgE titres have conventionally been attributed to long-lived IgE(+) plasma cells (PCs), this has not been directly and comprehensively tested. OBJECTIVE: To evaluate mechanisms underlying persistent IgE and allergic responses to food allergens...
February 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28216160/effect-of-cortisol-on-gonadotropin-inhibitory-hormone-gnih-in-the-cinnamon-clownfish-amphiprion-melanopus
#19
Young Jae Choi, Hamid R Habibi, Gyung-Suk Kil, Min-Min Jung, Cheol Young Choi
Hypothalamic peptides, gonadotropin-releasing hormone (GnRH) and gonadotropin inhibitory hormone (GnIH), play pivotal roles in the control of reproduction and gonadal maturation in fish. In the present study we tested the possibility that stress-mediated reproductive dysfunction in teleost may involve changes in GnRH and GnIH activity. We studied expression of brain GnIH, GnIH-R, seabream GnRH (sbGnRH), as well as circulating levels of follicle stimulating hormone (FSH), luteinizing hormone (LH) and cortisol in the cinnamon clownfish, Amphiprion melanopus...
February 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28215742/identification-of-grass-carp-ctenopharyngodon-idella-xbp1s-as-a-primary-member-in-er-stress
#20
Xiangqin Wang, Yichuan Mi, Bin Zhong, Huiling Mao, Yiqi Wan, Tao Zhang, Haizhou Wang, Chengyu Hu
X-box binding protein 1 (XBP1), a vital basic leucine zipper transcription factor for the related gene transcription in endoplasmic reticulum (ER) stress, belongs to the CREB/ATF family. In mammals, XBP1S is the activated one of XBP1 isoform. In order to study the role of fish XBP1S, we cloned and identified the XBP1S (KU509247) from grass carp (Ctenopharyngodon idella) (named CiXBP1S) by homologous cloning and RACE technique. The full length of CiXBP1S is 1694 bp along with 124 bp of 5' UTR, 418 bp of 3' UTR and the longest open reading frame (1152 bp) encoding a polypeptide of 383 amino acids with a well conserved DNA binding domain (BRLZ domain)...
February 16, 2017: Fish & Shellfish Immunology
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