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https://www.readbyqxmd.com/read/28100304/iron-responsive-like-elements-in-the-parasite-entamoeba-histolytica
#1
Liliana Soto-Castro, Laura Yuliana Plata-Guzman, Elisa Elvira Figueroa-Angulo, Jaeson Calla-Choque, Magda Reyes-Lopez, Mireya DE LA Garza, Nidia Leon-Sicairos, Jose Antonio Garzon-Tiznado, Rossana Arroyo, Claudia Leon-Sicairos
In Entamoeba histolytica, iron modulates virulence and gene expression via unknown regulatory mechanisms. The existence of a posttranscriptional iron regulatory system parallel to the IRE/IRP (Iron-Responsive Element/Iron Regulatory Protein) system in the protozoan Trichomonas vaginalis has recently been reported. Due to their evolutionary closeness and the importance of iron for growth and virulence in these protozoa, we hypothesized the existence of an IRE/IRP-like mechanism in E. histolytica. To determine the presence of IRE-like elements in some mRNAs from this parasite, we performed in silico analyses of the 5'- and 3'-UTRs of mRNAs encoding virulence factors and cytoskeleton, ribosomal, and metabolism proteins...
January 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28089548/nanoformulation-of-synergistic-tlr-ligands-to-enhance-vaccination-against-entamoeba-histolytica
#2
Mayuresh M Abhyankar, Zannatun Noor, Mark A Tomai, James Elvecrog, Christopher B Fox, William A Petri
Diarrheal infectious diseases represent a major cause of global morbidity and mortality. There is an urgent need for vaccines against diarrheal pathogens, especially parasites. Modern subunit vaccines rely on combining a highly purified antigen with an adjuvant to increase their efficacy. In the present study, we evaluated the ability of a nanoliposome adjuvant system to trigger a strong mucosal immune response to the Entamoeba histolytica Gal/GalNAc lectin LecA antigen. CBA/J mice were immunized with alum, emulsion or liposome based formulations containing synthetic TLR agonists...
January 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28087959/a-study-on-hla-dr-dq-typing-in-adult-malay-patients-with-acute-amoebic-liver-abscess
#3
Z Nazli, N M S Azira, M Zeehaida, Z Nik Zairi, Y Nurul Khaiza, Y Maya Mazuwin
INTRODUCTION: Amoebiasis is a parasitic disease caused by Entamoeba histolytica that may lead to death in developing countries. Few important risk factors have been identified in the development of amoebic liver abscess (ALA). There are limited reports that suggest an association between antigens of the major histocompatibility complex (MHC) particularly class II antigens and ALA development. This present work aimed at studying the possible association of HLA antigens with ALA and disease severity...
December 2016: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28070012/prevalence-and-risk-factors-for-intestinal-parasitic-infection-in-schoolchildren-in-battambang-cambodia
#4
Chien-Wei Liao, Kuan-Chih Chiu, I-Chen Chiang, Po-Ching Cheng, Ting-Wu Chuang, Juo-Han Kuo, Yun-Hung Tu, Chia-Kwung Fan
Most intestinal parasitic infections (IPIs), commonly endemic in tropical resource-poor developing countries, are neglected tropical diseases. Parasitic infections and malnutrition are most commonly found in children. We determined the prevalence of IPIs and the risk factors in Battambang Province, northwestern Cambodia, from August to September 2015. This study collected 308 valid questionnaires and specimens from Dontri (173, 56.2%) and Kon Kaêk (135, 43.8%) primary schools. All stool samples were examined using Chang's Feces Examination Apparatus through the merthiolate-iodine-formaldehyde technique...
January 9, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28069814/muc2-mucin-and-butyrate-contribute-to-the-synthesis-of-the-antimicrobial-peptide-cathelicidin-in-response-to-e-histolytica-and-dss-induced-colitis
#5
Eduardo R Cobo, Vanessa Kissoon-Singh, France Moreau, Ravi Holani, Kris Chadee
Embedded in the colonic mucus are cathelicidins, small cationic peptides secreted by colonic epithelial cells. Humans and mice have one cathelicidin-related antimicrobial peptide (CRAMP), LL-37/hCAP-18 and Cramp respectively, with related structure and functions. Altered production of MUC2 mucin and antimicrobial peptides is characteristic of intestinal amebiasis. The interactions between MUC2 mucin and cathelicidins in conferring innate immunity against Entamoeba histolytica (Eh) are not well characterized...
January 9, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28061872/amoebic-liver-abscess-in-northern-sri-lanka-first-report-of-immunological-and-molecular-confirmation-of-aetiology
#6
Selvam Kannathasan, Arumugam Murugananthan, Thirunavukarasu Kumanan, Devika Iddawala, Nilanthi Renuka de Silva, Nadarajah Rajeshkannan, Rashidul Haque
BACKGROUND: Since 1985, amoebic liver abscess (ALA) has been a public health problem in northern Sri Lanka. Clinicians arrive at a diagnosis based on clinical and ultrasonographic findings, which cannot differentiate pyogenic liver abscess (PLA) from ALA. As the treatment and outcome of the ALA and PLA differs, determining the etiological agent is crucial. METHODS: All clinically diagnosed ALA patients admitted to the Teaching Hospital (TH) in Jaffna during the study period were included and the clinical features, haematological parameters, and ultrasound scanning findings were obtained...
January 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28030342/molecular-diagnosis-of-entamoeba-spp-versus-microscopy-in-the-great-cairo
#7
Mohamed H Roshdy, Nour M Abd El-Kader, Marwa Ali-Tammam, Isabel Fuentes, Magdy M Mohamed, Nabila A El-Sheikh, Jose Miguel Rubio
Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as Entamoeba dispar, and Entamoeba moshkovskii. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E...
March 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/28027871/new-antiprotozoal-agents-synthesis-and-biological-evaluation-of-different-4-7-chloroquinolin-4-yl-piperazin-1-yl-pyrrolidin-2-yl-methanone-derivatives
#8
Mohammad Fawad Ansari, Faisal Hayat, Afreen Inam, Fatima Kathrada, Robyn L van Zyl, Maureen Coetzee, Kamal Ahmad, Dongyun Shin, Amir Azam
In an endeavor to develop efficacious antiprotozoal agents 4-(7-chloroquinolin-4-yl) piperazin-1-yl)pyrrolidin-2-yl)methanone derivatives (5-14) were synthesized, characterized and biologically evaluated for antiprotozoal activity. The compounds were screened in vitro against the HM1: IMSS strain of Entamoeba histolytica and NF54 chloroquine-sensitive strain of Plasmodium falciparum. Among the synthesized compounds six exhibited promising antiamoebic activity with IC50 values (0.14-1.26μM) lower than the standard drug metronidazole (IC50 1...
December 18, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28018950/data-on-rad51-amino-acid-sequences-from-higher-and-lower-eukaryotic-model-organisms-and-parasites
#9
Andrew A Kelso, Steven D Goodson, Lesly A Temesvari, Michael G Sehorn
This paper contains data related to the research article titled "Characterization of the recombination activities of the Entamoeba histolytica Rad51 recombinase" (Kelso et al., in press) [1]. The known and putative amino acid sequence of Rad51, the central enzyme of homologous recombination, from nineteen different higher and lower eukaryotic organisms was analyzed. Here, we show amino acid conservation using a multiple sequence alignment, overall sequence identities using a percent identity matrix, and the evolutionary relationship between organisms using a neighbor-joining tree...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28007484/diagnosis-of-intestinal-parasites-in-a-rural-community-of-venezuela-advantages-and-disadvantages-of-using-microscopy-or-rt-pcr
#10
Renzo Nino Incani, Elizabeth Ferrer, Denise Hoek, Robbert Ramak, Jeroen Roelfsema, Lapo Mughini-Gras, Titia Kortbeek, Elena Pinelli
A cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time PCR (RT-PCR) for 14 intestinal parasites in a Venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. A total of 228 participants were included in this study. A multiplex RT-PCR was used for the detection of Dientamoeba fragilis, Giardia intestinalis, Cryptosporidium sp. and a monoplex RT-PCR for Entamoeba histolytica...
December 19, 2016: Acta Tropica
https://www.readbyqxmd.com/read/28002502/ehnpc1-and-ehnpc2-proteins-participate-in-trafficking-of-exogenous-cholesterol-in-entamoeba-histolytica-trophozoites-relevance-for-phagocytosis
#11
Jeni Bolaños, Abigail Betanzos, Rosario Javier-Reyna, Guillermina García-Rivera, Miriam Huerta, Jonnatan Pais-Morales, Arturo González-Robles, Mario A Rodríguez, Michael Schnoor, Esther Orozco
Entamoeba histolytica, the highly phagocytic protozoan causative of human amoebiasis lacks the machinery to synthesize cholesterol. Here, we investigated the presence of NPC1 and NPC2 proteins in this parasite, which are involved in cholesterol trafficking in mammals. Bioinformatics analysis revealed one Ehnpc1 and two Ehnpc2 genes. EhNPC1 appeared as a transmembrane protein and both EhNPC2 as peripheral membrane proteins. Molecular docking predicted that EhNPC1 and EhNPC2 bind cholesterol and interact with each other...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27990612/-1-h-13-c-and-15-n-nmr-assignments-of-an-unusual-ca-2-binding-protein-from-entamoeba-histolytica-in-its-apo-form
#12
Deepshikha Verma, Mutyala Sakuntala, Aruna Murmu, Alok Bhattacharya, Kandala V R Chary
We report almost complete sequence specific (1)H, (13)C and (15)N NMR assignments of an unusual Ca(2+)-binding protein from Entamoeba histolytica (EhCaBP6) in its apo form as a prelude to its structural and functional characterization.
December 19, 2016: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/27975729/antiprotozoal-activity-against-entamoeba-histolytica-of-furocoumarins-isolated-from-ruta-chalepensis
#13
R Quintanilla-Licea, B D Mata-Cárdenas, J Vargas-Villarreal, A F Bazaldúa-Rodríguez, M J Verde-Star
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27965649/the-12-gastrointestinal-pathogens-spectrum-of-acute-infectious-diarrhea-in-a-sentinel-hospital-shenzhen-china
#14
Hongwei Shen, Jinjin Zhang, Yinghui Li, Sirou Xie, Yixiang Jiang, Yanjie Wu, Yuhui Ye, Hong Yang, Haolian Mo, Chaoman Situ, Qinghua Hu
Acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. The pathogen spectrum may differ among different regions and seasons. To investigate the etiology of acute diarrhea in Shenzhen, a prospective study was conducted from August 2014 to September 2015. Stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. The 19 pathogens were detected by conventional culture method or multiplex PCR assay, which included five viruses (rotavirus, adenovirus, sapovirus, norovirus, and astrovirus), 11 bacterial pathogens (Salmonella, Campylobacter jejuni, Shigella, Listeria monocytogenes, Vibrio parahaemolyticus, Vibrio cholera, Enterohemorrhagic (EHEC), enteropathogenic (EPEC), enteroinvasive (EIEC), enterotoxigenic (ETEC); and enteroaggregative Escherichia coli (EAEC)) and three parasites (Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27931200/amoebic-liver-abscess-an-unusual-cause-for-a-right-iliac-fossa-mass-a-case-report
#15
Vithiya Ratnasamy, Kumanan Thirunavukarasu, Kannathasan Selvam, Murugananthan Arumugam
BACKGROUND: Amoebic liver abscess is the most common extra intestinal manifestation of amoebiasis in tropical countries. It usually presents with right hypochondrial pain, fever and anorexia. Amoebic liver abscess has gained clinical significance due to the wide variety of clinical presentations which can cause diagnostic dilemmas and high mortality in untreated cases. CASE PRESENTATION: We report a case of a 63-year-old male with a history of anorexia for 3 weeks, fever for 4 days and examination findings of tender hepatomegaly with a liver span of 15 cm in the mid clavicular line and a firm irregular mass in the right iliac fossa...
December 8, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27930733/phosphorylation-of-eukaryotic-initiation-factor-2%C3%AE-during-stress-and-encystation-in-entamoeba-species
#16
Holland M Hendrick, Brenda H Welter, Matthew A Hapstack, Steven E Sykes, William J Sullivan, Lesly A Temesvari
Entamoeba histolytica is an enteric pathogen responsible for amoebic dysentery and liver abscess. It alternates between the host-restricted trophozoite form and the infective environmentally-stable cyst stage. Throughout its lifecycle E. histolytica experiences stress, in part, from host immune pressure. Conversion to cysts is presumed to be a stress-response. In other systems, stress induces phosphorylation of a serine residue on eukaryotic translation initiation factor-2α (eIF2α). This inhibits eIF2α activity resulting in a general decline in protein synthesis...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27930683/deep-insight-into-the-phosphatomes-of-parasitic-protozoa-and-a-web-resource-protozphosdb
#17
Tamanna Anwar, Samudrala Gourinath
Phosphorylation dynamically regulates the function of proteins by maintaining a balance between protein kinase and phosphatase activity. A comprehensive understanding of the role phosphatases in cellular signaling is lacking in case of protozoans of medical and veterinary importance worldwide. The drugs used to treat protozoal diseases have many undesired effects and the development of resistance, highlights the need for new effective and safer antiprotozoal agents. In the present study we have analyzed phosphatomes of 15 protozoans of medical significance...
2016: PloS One
https://www.readbyqxmd.com/read/27930312/metal-dependence-and-branched-rna-cocrystal-structures-of-the-rna-lariat-debranching-enzyme-dbr1
#18
Nathaniel E Clark, Adam Katolik, Kenneth M Roberts, Alexander B Taylor, Stephen P Holloway, Jonathan P Schuermann, Eric J Montemayor, Scott W Stevens, Paul F Fitzpatrick, Masad J Damha, P John Hart
Intron lariats are circular, branched RNAs (bRNAs) produced during pre-mRNA splicing. Their unusual chemical and topological properties arise from branch-point nucleotides harboring vicinal 2',5'- and 3',5'-phosphodiester linkages. The 2',5'-bonds must be hydrolyzed by the RNA debranching enzyme Dbr1 before spliced introns can be degraded or processed into small nucleolar RNA and microRNA derived from intronic RNA. Here, we measure the activity of Dbr1 from Entamoeba histolytica by using a synthetic, dark-quenched bRNA substrate that fluoresces upon hydrolysis...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27923058/effect-of-mass-stool-examination-and-mass-treatment-for-decreasing-intestinal-helminth-and-protozoan-infection-rates-in-bolivian-children-a-cross-sectional-study
#19
Takao Asai, Claudia Còrdova Vidal, Wilma Strauss, Toshikazu Ikoma, Kazuo Endoh, Masaharu Yamamoto
Bolivia is one of the countries with a high intestinal helminth and protozoan infection rate. Despite the high prevalence of the parasitic infection, nationwide preventive measures for Bolivian children have not yet been implemented. We evaluated the effect of mass stool examination and treatment as a strategy for decreasing the infection rate. This study was conducted between 2013 and 2015 in children aged 2-18 years. A total of 2,033 stool samples (575 in 2013, 815 in 2014 and 642 in 2015) were collected and examined using the formalin-ether medical sedimentation method...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27912173/improved-anticancer-and-antiparasitic-activity-of-new-lawsone-mannich-bases
#20
Katharina Mahal, Aamir Ahmad, Florian Schmitt, Julia Lockhauserbäumer, Kathrin Starz, Rohan Pradhan, Subhash Padhye, Fazlul H Sarkar, Waleed S Koko, Rainer Schobert, Klaus Ersfeld, Bernhard Biersack
Substituted lawsone Mannich bases 2a-e, 3a-e and 4a-e were prepared and tested for their biological activities. The new fatty alkyl substituted compounds 2a-c exhibited strong and selective growth inhibitory activities in the low one-digit micromolar and sub-micromolar range against a panel of human cancer cell lines associated with ROS formation. In addition, compounds 2a-c revealed sub-micromolar anti-trypanosomal activities against parasitic Trypanosoma brucei brucei cells via deformation of the microtubule cytoskeleton...
November 22, 2016: European Journal of Medicinal Chemistry
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