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https://www.readbyqxmd.com/read/27899510/identification-and-in-silico-modeling-of-enhancers-reveals-new-features-of-the-cardiac-differentiation-network
#1
Denis Seyres, Yad Ghavi-Helm, Guillaume Junion, Ouarda Taghli-Lamallem, Céline Guichard, Laurence Röder, Charles Girardot, Eileen E M Furlong, Laurent Perrin
Developmental patterning and tissue formation are regulated through complex gene regulatory networks (GRNs) driven through the action of transcription factors (TFs) converging on enhancer elements. Here, as a point of entry to dissect the poorly defined GRN underlying cardiomyocyte differentiation, we apply an integrated approach to identify active enhancers and TFs involved in Drosophila heart development. The Drosophila heart consists of 104 cardiomyocytes, representing less than 0.5% of all cells in the embryo...
December 1, 2016: Development
https://www.readbyqxmd.com/read/27857486/blebs-barriers-and-bagpipes-why-is-it-so-hard
#2
Michael Coote
: Doug Johnson was a clinician-scientist who made great contributions to the understanding of outflow from the eye. This lecture is in honour of Doug and explores the author's understanding of outflow in the surgical context. HOW TO CITE THIS ARTICLE: Coote M. Blebs, Barriers, and Bagpipes: Why is It so Hard? J Curr Glaucoma Pract 2016; 10(3):79-84.
September 2016: Journal of Current Glaucoma Practice
https://www.readbyqxmd.com/read/27552781/bagpipe-lung-a-new-type-of-interstitial-lung-disease
#3
Jenny King, Malcolm Richardson, Anne-Marie Quinn, Jayne Holme, Nazia Chaudhuri
No abstract text is available yet for this article.
August 22, 2016: Thorax
https://www.readbyqxmd.com/read/27253464/cartilage-specific-and-cre-dependent-nkx3-2-overexpression-in-vivo-causes-skeletal-dwarfism-by-delaying-cartilage-hypertrophy
#4
Da-Un Jeong, Je-Yong Choi, Dae-Won Kim
Nkx3.2, the vertebrate homologue of Drosophila bagpipe, has been implicated as playing a role in chondrogenic differentiation. In brief, Nkx3.2 is initially expressed in chondrocyte precursor cells and later during cartilage maturation, its expression is diminished in hypertrophic chondrocytes. In addition to Nkx3.2 expression analyses, previous studies using ex vivo chick embryo cultures and in vitro cell cultures have suggested that Nkx3.2 can suppress chondrocyte hypertrophy. However, it has never been demonstrated that Nkx3...
January 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/26663763/evaluation-of-bias-on-the-assessment-of-diet-breadth-of-herbivorous-insects-using-molecular-methods
#5
Gissela de la Cadena, Anna Papadopoulou, Jean-Michel Maes, Jesús Gómez-Zurita
The interactions between herbivores and their host plants play a key role in ecological processes. Understanding the width and nature of these interactions is fundamental to ecology and conservation. Recent research on DNA-based inference of trophic associations suggests that the host range of phytophagous insects in the tropics may be wider than previously thought based on traditional observation. However, the reliability of molecular inference of ecological associations, still strongly dependent on PCR and thus exposed to the risk of contamination with environmental DNA, is under debate...
December 11, 2015: Insect Science
https://www.readbyqxmd.com/read/24911136/conjunction-illusions-and-conjunction-fallacies-in-episodic-memory
#6
C J Brainerd, Robyn E Holliday, Koyuki Nakamura, Valerie F Reyna
Recent research on the overdistribution principle implies that episodic memory is infected by conjunction illusions. These are instances in which an item that was presented in a single context (e.g., List 1) is falsely remembered as having been presented in multiple contexts (e.g., List 1 and List 2). Robust conjunction illusions were detected in source-monitoring designs in which conjunctive probes ("Was bagpipe presented on List 1 and List 2?") were added to the traditional nonconjunctive probes ("Was bagpipe presented on List 1?")...
November 2014: Journal of Experimental Psychology. Learning, Memory, and Cognition
https://www.readbyqxmd.com/read/24666885/automated-dna-based-plant-identification-for-large-scale-biodiversity-assessment
#7
Anna Papadopoulou, Douglas Chesters, Indiana Coronado, Gissela De la Cadena, Anabela Cardoso, Jazmina C Reyes, Jean-Michel Maes, Ricardo M Rueda, Jesús Gómez-Zurita
Rapid degradation of tropical forests urges to improve our efficiency in large-scale biodiversity assessment. DNA barcoding can assist greatly in this task, but commonly used phenetic approaches for DNA-based identifications rely on the existence of comprehensive reference databases, which are infeasible for hyperdiverse tropical ecosystems. Alternatively, phylogenetic methods are more robust to sparse taxon sampling but time-consuming, while multiple alignment of species-diagnostic, typically length-variable, markers can be problematic across divergent taxa...
January 2015: Molecular Ecology Resources
https://www.readbyqxmd.com/read/23727297/expression-pattern-of-the-homeotic-gene-bapx1-during-early-chick-gastrointestinal-tract-development
#8
Sandrine Faure, Maxime Georges, Jennifer McKey, Sébastien Sagnol, Pascal de Santa Barbara
Regulation of the Bone Morphogenetic Protein (BMP) signaling pathway is essential for the normal development of vertebrate gastrointestinal (GI) tract, but also for the differentiation of the digestive mesenchymal layer into smooth muscles and submucosal layer. Different studies demonstrated that Bapx1 (for bagpipe homeobox homolog 1) negatively regulates the BMP pathway, but its precise expression pattern during the development and the differentiation of the GI tract mesenchyme actually remains to be examined...
December 2013: Gene Expression Patterns: GEP
https://www.readbyqxmd.com/read/22216134/comparison-of-ires-and-f2a-based-locus-specific-multicistronic-expression-in-stable-mouse-lines
#9
Hsiao Yun Chan, Sivakamasundari V, Xing Xing, Petra Kraus, Sook Peng Yap, Patricia Ng, Siew Lan Lim, Thomas Lufkin
Efficient and stoichiometric expression of genes concatenated by bi- or multi-cistronic vectors has become an invaluable tool not only in basic biology to track and visualize proteins in vivo, but also for vaccine development and in the clinics for gene therapy. To adequately compare, in vivo, the effectiveness of two of the currently popular co-expression strategies - the internal ribosome entry site (IRES) derived from the picornavirus and the 2A peptide from the foot-and-mouth disease virus (FDMV) (F2A), we analyzed two locus-specific knock-in mouse lines co-expressing SRY-box containing gene 9 (Sox9) and enhanced green fluorescent protein (EGFP) linked by the IRES (Sox9(IRES-EGFP)) or the F2A (Sox9(F2A-EGFP)) sequence...
2011: PloS One
https://www.readbyqxmd.com/read/21536897/fluctuating-and-sensory-induced-vasodynamics-in-rodent-cortex-extend-arteriole-capacity
#10
Patrick J Drew, Andy Y Shih, David Kleinfeld
Neural activity in the brain is followed by localized changes in blood flow and volume. We address the relative change in volume for arteriole vs. venous blood within primary vibrissa cortex of awake, head-fixed mice. Two-photon laser-scanning microscopy was used to measure spontaneous and sensory evoked changes in flow and volume at the level of single vessels. We find that arterioles exhibit slow (<1 Hz) spontaneous increases in their diameter, as well as pronounced dilation in response to both punctate and prolonged stimulation of the contralateral vibrissae...
May 17, 2011: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/20112453/conservation-of-gene-linkage-in-dispersed-vertebrate-nk-homeobox-clusters
#11
Karl R Wotton, Frida K Weierud, José L Juárez-Morales, Lúcia E Alvares, Susanne Dietrich, Katharine E Lewis
Nk homeobox genes are important regulators of many different developmental processes including muscle, heart, central nervous system and sensory organ development. They are thought to have arisen as part of the ANTP megacluster, which also gave rise to Hox and ParaHox genes, and at least some NK genes remain tightly linked in all animals examined so far. The protostome-deuterostome ancestor probably contained a cluster of nine Nk genes: (Msx)-(Nk4/tinman)-(Nk3/bagpipe)-(Lbx/ladybird)-(Tlx/c15)-(Nk7)-(Nk6/hgtx)-(Nk1/slouch)-(Nk5/Hmx)...
October 2009: Development Genes and Evolution
https://www.readbyqxmd.com/read/20072845/conservation-of-gene-linkage-in-dispersed-vertebrate-nk-homeobox-clusters
#12
Karl R Wotton, Frida K Weierud, José L Juárez-Morales, Lúcia E Alvares, Susanne Dietrich, Katharine E Lewis
Nk homeobox genes are important regulators of many different developmental processes including muscle, heart, central nervous system and sensory organ development. They are thought to have arisen as part of the ANTP megacluster, which also gave rise to Hox and ParaHox genes, and at least some NK genes remain tightly linked in all animals examined so far. The protostome-deuterostome ancestor probably contained a cluster of nine Nk genes: (Msx)-(Nk4/tinman)-(Nk3/bagpipe)-(Lbx/ladybird)-(Tlx/c15)-(Nk7)-(Nk6/hgtx)-(Nk1/slouch)-(Nk5/Hmx)...
January 14, 2010: Development Genes and Evolution
https://www.readbyqxmd.com/read/19306868/sox9-directly-promotes-bapx1-gene-expression-to-repress-runx2-in-chondrocytes
#13
Satoshi Yamashita, Masataka Andoh, Hiroe Ueno-Kudoh, Tempei Sato, Shigeru Miyaki, Hiroshi Asahara
The transcription factor, Sry-related High Mobility Group (HMG) box containing gene 9 (Sox9), plays a critical role in cartilage development by initiating chondrogenesis and preventing the subsequent maturation process called chondrocyte hypertrophy. This suppression mechanism by Sox9 on late-stage chondrogenesis partially results from the inhibition of Runt-related transcription factor 2 (Runx2), the main activator of hypertrophic chondrocyte differentiation. However, the precise mechanism by which Sox9 regulates late chondrogenesis is poorly understood...
August 1, 2009: Experimental Cell Research
https://www.readbyqxmd.com/read/18296735/structural-and-functional-analysis-of-domains-mediating-interaction-between-the-bagpipe-homologue-nkx3-1-and-serum-response-factor
#14
Yan Zhang, Rebecca A Fillmore, Warren E Zimmer
Nkx3.1 is a member of the NK2 class of homeodomain proteins and is expressed in development, being an early marker of the sclerotome and prostate gland. It has been shown to be a critical factor for prostate differentiation and function. Previous studies suggested that Nkx3.1 interacts with Serum Response Factor (SRF) to transactivate the Smooth Muscle gamma-Actin (SMGA) promoter. In studies presented here, we examined the molecular mechanisms underlying the functional synergy of these factors upon SMGA transcription...
March 2008: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/17908931/temporal-chip-on-chip-reveals-biniou-as-a-universal-regulator-of-the-visceral-muscle-transcriptional-network
#15
Janus S Jakobsen, Martina Braun, Jeanette Astorga, E Hilary Gustafson, Thomas Sandmann, Michal Karzynski, Peter Carlsson, Eileen E M Furlong
Smooth muscle plays a prominent role in many fundamental processes and diseases, yet our understanding of the transcriptional network regulating its development is very limited. The FoxF transcription factors are essential for visceral smooth muscle development in diverse species, although their direct regulatory role remains elusive. We present a transcriptional map of Biniou (a FoxF transcription factor) and Bagpipe (an Nkx factor) activity, as a first step to deciphering the developmental program regulating Drosophila visceral muscle development...
October 1, 2007: Genes & Development
https://www.readbyqxmd.com/read/17511863/hand-is-a-direct-target-of-the-forkhead-transcription-factor-biniou-during-drosophila-visceral-mesoderm-differentiation
#16
Dmitry Popichenko, Julia Sellin, Marek Bartkuhn, Achim Paululat
BACKGROUND: The visceral trunk mesoderm in Drosophila melanogaster develops under inductive signals from the ectoderm. This leads to the activation of the key regulators Tinman, Bagpipe and Biniou that are crucial for specification of the circular visceral muscles. How further differentiation is regulated is widely unknown, therefore it seems to be essential to identify downstream target genes of the early key regulators. In our report we focus on the analysis of the transcriptional control of the highly conserved transcription factor Hand in circular visceral muscle cells, providing evidence that the hand gene is a direct target of Biniou...
2007: BMC Developmental Biology
https://www.readbyqxmd.com/read/16937892/the-aha-effect-in-groups-and-other-dynamic-learning-contexts
#17
Theodore W Wills, Sarah Estow, Sal A Soraci, Julio Garcia
The aha effect is a memory advantage for initially ambiguous stimuli (e.g., "The notes were sour because the seam split.") that are subsequently resolved ("Bagpipe") over stimuli that are comprehensible from the outset (see P M. Auble, J. J. Franks, & S. A. Soraci, 1979; T. W. Wills, S. A. Soraci, R. A. Chechile, & H. A. Taylor, 2000). The authors examined the influence of learning context on the aha effect by manipulating whether generative tasks were completed in a group setting or alone. In Experiment 1, participants in a group context either discussed difficult aha sentences, or overheard the discussion...
July 2006: Journal of General Psychology
https://www.readbyqxmd.com/read/16791844/bapx1-homeobox-gene-gain-of-function-mice-show-preaxial-polydactyly-and-activated-shh-signaling-in-the-developing-limb
#18
Carla Tribioli, Thomas Lufkin
To explore Bapx1 homeobox gene function in embryonic control of development, we employed a gain-of-function approach to complement our previous loss-of-function mutant analysis. We show that transgenic mice overexpressing Bapx1 are affected by skeletal defects including hindlimb preaxial polydactyly and tibial hypoplasia. Bapx1 overexpression generates limb anteroposterior patterning defects including induction of Shh signaling and ectopic activation of functions downstream of Shh signaling into the anterior region of the autopod...
September 2006: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/16638295/-the-effect-of-subglottic-secretion-drainage-on-prevention-of-ventilator-associated-lower-airway-infection
#19
RANDOMIZED CONTROLLED TRIAL
Shu-hong Liu, Xi-xin Yan, Shuang-qing Cao, Shu-chang An, Li-juan Zhang
OBJECTIVE: To assess the influence of subglottic secretion drainage (SSD) on the morbidity of ventilator-associated pneumonia (VAP) in mechanically ventilated patients. METHODS: All studied patients who received mechanical ventilation (MV) estimated for more than 48 hours were intubated with a special type endotracheal tube with a small-bore cannula in the wall for SSD. The patients were randomly divided into two groups receiving SSD (group A) and usual care (non-SSD, group B) respectively...
January 2006: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/16004970/the-homeodomain-of-tinman-mediates-homo-and-heterodimerization-of-nk-proteins
#20
Stéphane Zaffran, Manfred Frasch
Cardiac development requires the action of transcription factors, which control the specification and differentiation of cardiac cell types. One of these factors, encoded by the homeobox gene tinman (tin), is essential for the specification of all cardiac cells in Drosophila. An increasing number of examples show that protein-protein interactions can be important for determining the specific transcriptional activities of homeodomain proteins, in addition to their binding to specific DNA target sites. Here, we show that Tin and Bagpipe (Bap), another homeodomain protein, form homo- and heterodimeric complexes...
August 26, 2005: Biochemical and Biophysical Research Communications
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