Connor Davison, Hamelynn Harzman, Jessie Nicholson, Seth Entriken, Kierinn Mobley, Abigail Krull, Manik Singhal, Caleb Skow, Nathan Matthews, Lindsey Kopp, Benjamin Gillette, Tyler J Weide, Jana R Hukvari, Sofia C P Stumpf, Olivia M Feldmann, Maura McGrail, Renu Srivastava, Jeffrey J Essner
Tjp1a and other tight junction and adherens proteins play important roles in cell-cell adhesion, scaffolding, and forming seals between cells in epithelial and endothelial tissues. In this study, we labeled Tjp1a of zebrafish with the monomeric red fluorescent protein (mRFP) using CRISPR/Cas9-mediated targeted integration of biotin-labeled polymerase chain reaction (PCR) generated templates. Labeling Tjp1a with RFP allowed us to follow membrane and junctional dynamics of epithelial and endothelial cells throughout zebrafish embryo development...
April 2024: Zebrafish