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https://www.readbyqxmd.com/read/28597430/identification-of-drought-stress-related-proteins-from-1s-l-1b-chromosome-substitution-line-of-wheat-variety-chinese-spring
#1
Jiaxing Zhou, Chaoying Ma, Shoumin Zhen, Min Cao, Friedich J Zeller, Sai L K Hsam, Yueming Yan
BACKGROUND: Wheat, one of the most important crops, has a detrimental effect on both yield and quality under drought stress. As our preliminary experiment showed that the Chinese Spring wheat-Aegilops longissima chromosome substitution line CS-1S(l) (1B) had a better drought tolerance than CS, the substitution line CS-1S(l)(1B) was used to identify drought stress related proteins by means of a comparative proteome approach in this work. Our present study aimed to explore the gene resources for drought resistance in 1S(l) genome...
December 2016: Botanical Studies (Taipei, Taiwan)
https://www.readbyqxmd.com/read/28370981/in-vitro-assessment-of-a-novel-hypothermically-stored-amniotic-membrane-for-use-in-a-chronic-wound-environment
#2
John P McQuilling, Jeremy B Vines, Katie C Mowry
Chronic wounds require extensive healing time and place patients at risk of infection and amputation. Recently, a fresh hypothermically stored amniotic membrane (HSAM) was developed and has subsequently shown promise in its ability to effectively heal chronic wounds. The purpose of this study is to investigate the mechanisms of action that contribute to wound-healing responses observed with HSAM. A proteomic analysis was conducted on HSAM, measuring 25 growth factors specific to wound healing within the grafts...
March 29, 2017: International Wound Journal
https://www.readbyqxmd.com/read/28045339/imaging-biodistribution-and-efficacy-evaluation-of-188-re-human-serum-albumin-microspheres-via-intraarterial-route-in-an-orthotopic-hepatoma-model
#3
Liang-Cheng Chen, Ya-Jen Chang, Su-Jung Chen, Wan-Chi Lee, Chih-Hsien Chang, Te-Wei Lee, Jui-Hung Shien
PURPOSE: Liver cancer is the second most common cause of death worldwide. This study was to investigate the SPECT/CT, ultrasound, biodistribution and therapeutic evaluation of (188)Re-human serum albumin microspheres ((188)Re-HSAM) in the GP7TB orthotopic hepatoma rat model. MATERIALS AND METHODS: HSAM was labeled with (188)Re by using a home-made kit and microwave system. The (188)Re-HSAM was administered via intraarterial route. The in vivo distribution of (188)Re-HSAM was determined by biodistribution analysis and nanoSPECT/CT system...
May 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/27222247/home-safety-assessment-and-modification-to-reduce-injurious-falls-in-community-dwelling-older-adults-cost-utility-and-equity-analysis
#4
Frank Pega, Giorgi Kvizhinadze, Tony Blakely, June Atkinson, Nick Wilson
BACKGROUND: This study aimed to improve on previous modelling work to determine the health gain, cost-utility and health equity impacts from home safety assessment and modification (HSAM) for reducing injurious falls in older people. METHODS: The model was a Markov macrosimulation one that estimated quality-adjusted life-years (QALYs) gained. The setting was a country with detailed epidemiological and cost data (New Zealand (NZ)) for 2011. A health system perspective was taken and a discount rate of 3% was used (for both health gain and costs)...
May 24, 2016: Injury Prevention: Journal of the International Society for Child and Adolescent Injury Prevention
https://www.readbyqxmd.com/read/27192353/secondary-structures-of-ubiquitin-ions-soft-landed-onto-self-assembled-monolayer-surfaces
#5
Qichi Hu, Julia Laskin
The secondary structures of multiply charged ubiquitin ions soft-landed onto self-assembled monolayer (SAM) surfaces were studied using in situ infrared reflection-absorption spectroscopy (IRRAS). Two charge states of ubiquitin, 5+ and 13+, were mass selected separately from a mixture of different charge states produced by electrospray ionization (ESI). The low 5+ charge state represents a nativelike folded state of ubiquitin, while the high 13+ charge state assumes an extended, almost linear conformation. Each of the two charge states was soft-landed onto a CH3- and COOH-terminated SAM of alkanethiols on gold (HSAM and COOH-SAM)...
June 9, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/26982996/a-cognitive-assessment-of-highly-superior-autobiographical-memory
#6
Aurora K R LePort, Shauna M Stark, James L McGaugh, Craig E L Stark
Highly Superior Autobiographical Memory (HSAM) is characterised as the ability to accurately recall an exceptional number of experiences and their associated dates from events occurring throughout much of one's lifetime. The source of this ability has only begun to be explored. The present study explores whether other enhanced cognitive processes may be critical influences underlying HSAM abilities. We investigated whether enhanced abilities in the domains of verbal fluency, attention/inhibition, executive functioning, mnemonic discrimination, perception, visual working memory, or the processing of and memory for emotional details might contribute critically to HSAM...
February 2017: Memory
https://www.readbyqxmd.com/read/26314991/individual-differences-and-correlates-of-highly-superior-autobiographical-memory
#7
Lawrence Patihis
Highly superior autobiographical memory (HSAM) is a recently identified ability that has been difficult to explain with existing memory science. The present study measured HSAM participants' and age/gender-matched controls' on a number of behavioural measures to test three main hypotheses: imaginative absorption, emotional arousal, and sleep. HSAM participants were significantly higher than controls on the dispositions absorption and fantasy proneness. These two dispositions also were associated with a measure of HSAM ability within the hyperthymesia participants...
August 2016: Memory
https://www.readbyqxmd.com/read/25892594/severely-deficient-autobiographical-memory-sdam-in-healthy-adults-a-new-mnemonic-syndrome
#8
Daniela J Palombo, Claude Alain, Hedvig Söderlund, Wayne Khuu, Brian Levine
Recollection of previously experienced events is a key element of human memory that entails recovery of spatial, perceptual, and mental state details. While deficits in this capacity in association with brain disease have serious functional consequences, little is known about individual differences in autobiographical memory (AM) in healthy individuals. Recently, healthy adults with highly superior autobiographical capacities have been identified (e.g., LePort, A.K., Mattfeld, A.T., Dickinson-Anson, H., Fallon, J...
June 2015: Neuropsychologia
https://www.readbyqxmd.com/read/25748057/preparation-and-imaging-of-rhenium-188-labeled-human-serum-albumin-microsphere-in-orthotopic-hepatoma-rats
#9
Hsiao-Chiang Ni, Chia-Yu Yu, Su-Jung Chen, Liang-Cheng Chen, Chien-Hong Lin, Wan-Chi Lee, Cheng-Hui Chuang, Chung-Li Ho, Chih-Hsien Chang, Te-Wei Lee
OBJECTIVE: The present study relates to a method for preparing 188Re-labeled human serum albumin microspheres (HSAM) by 188Re(I)-tricarbonyl ion(188Re(OH2)3(CO)3)+). This radioactive particle can be subjected to radioembolization for liver tumor. METHODS: The particle sizes and conformations of HSA microspheres were analyzed by Particle sizes-Malvern mastersizer and Scanning Electron Microscope (SEM). For preparing 188Re(I)-tricarbonyl ion, the 188ReO4- was eluted from a 188W/188Re generator with saline...
May 2015: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/25476276/a-sensitive-capacitive-immunosensor-for-direct-detection-of-human-heart-fatty-acid-binding-protein-h-fabp
#10
Carmen-Marinela Mihailescu, Dana Stan, Rodica Iosub, Carmen Moldovan, Mihaela Savin
The fabrication of a capacitive interdigitated immunosensor (CID) based on a mixed self-assembled monolayer (mSAM) film for the direct detection of heart fatty-acid binding protein (h-FABP) without any labeling is described. The capacitance changes of mSAMs vs. homogenous ordered self-assembled monolayers (hSAMs) on gold work electrodes/covalently bonded antibodies/buffered medium are utilized for monitoring the specific antibody-antigen interaction. Capacitance measurements in the absence and presence of Faradaic currents were performed...
January 2015: Talanta
https://www.readbyqxmd.com/read/25332891/selective-internal-radiation-therapy-of-hepatic-tumors-procedural-implications-of-a-patent-hepatic-falciform-artery
#11
Juliane Schelhorn, Judith Ertle, Joerg F Schlaak, Stefan Mueller, Andreas Bockisch, Thomas Schlosser, Thomas Lauenstein
Selective internal radiation therapy (SIRT) using 90-yttrium is a local therapy for unresectable liver malignancies. Non-targeted 90-yttrium diversion via a patent hepatic falciform artery (HFA) is seen as risk for periprocedural complications. Therefore, this study aimed to evaluate the impact of a patent HFA on SIRT. 606 patients with SIRT between 2006 and 2012 were evaluated retrospectively. SIRT preparation was performed by digital subtraction angiography including (99m)Tc-HSAM administration and subsequent SPECT/CT...
2014: SpringerPlus
https://www.readbyqxmd.com/read/25310133/hemoglobin-albumin-cluster-incorporating-a-pt-nanoparticle-artificial-o2-carrier-with-antioxidant-activities
#12
Hitomi Hosaka, Risa Haruki, Kana Yamada, Christoph Böttcher, Teruyuki Komatsu
A covalent core-shell structured protein cluster composed of hemoglobin (Hb) at the center and human serum albumins (HSA) at the periphery, Hb-HSAm, is an artificial O2 carrier that can function as a red blood cell substitute. Here we described the preparation of a novel Hb-HSA3 cluster with antioxidant activities and its O2 complex stable in aqueous H2O2 solution. We used an approach of incorporating a Pt nanoparticle (PtNP) into the exterior HSA unit of the cluster. A citrate reduced PtNP (1.8 nm diameter) was bound tightly within the cleft of free HSA with a binding constant (K) of 1...
2014: PloS One
https://www.readbyqxmd.com/read/25273817/molecular-cloning-phylogenetic-analysis-and-expression-profiling-of-endoplasmic-reticulum-molecular-chaperone-bip-genes-from-bread-wheat-triticum-aestivum-l
#13
Jiantang Zhu, Pengchao Hao, Guanxing Chen, Caixia Han, Xiaohui Li, Friedrich J Zeller, Sai L K Hsam, Yingkao Hu, Yueming Yan
BACKGROUND: The endoplasmic reticulum chaperone binding protein (BiP) is an important functional protein, which is involved in protein synthesis, folding assembly, and secretion. In order to study the role of BiP in the process of wheat seed development, we cloned three BiP homologous cDNA sequences in bread wheat (Triticum aestivum), completed by rapid amplification of cDNA ends (RACE), and examined the expression of wheat BiP in wheat tissues, particularly the relationship between BiP expression and the subunit types of HMW-GS using near-isogenic lines (NILs) of HMW-GS silencing, and under abiotic stress...
October 1, 2014: BMC Plant Biology
https://www.readbyqxmd.com/read/25078057/characterization-of-powdery-mildew-resistance-and-linkage-studies-involving-the-pm3-locus-on-chromosome-1a-of-common-wheat-triticum-aestivum-l
#14
N B Ohnmar Hsam, Krzysztof Kowalczyk, Friedrich J Zeller, Sai L K Hsam
Genetic characterization of a new powdery mildew resistance gene in a common wheat line ATRI3004/79 was conducted by employing a set of Blumeria graminis tritici isolates collected from Europe. Monosomic analysis revealed that a major resistance gene is located on chromosome 1A. Allelism tests of the F2 and F3 populations with other previously known genes on 1A showed that the resistance gene in ATRI3004/79 is closely linked to the Pm3d resistance gene in the German cultivar Syros, whereas it segregated independently from Pm25...
February 2015: Journal of Applied Genetics
https://www.readbyqxmd.com/read/24581959/selective-internal-radiation-therapy-of-hepatic-tumours-is-coiling-of-the-gastroduodenal-artery-always-beneficial
#15
J Schelhorn, J Theysohn, J Ertle, J F Schlaak, S Mueller, A Bockisch, T Lauenstein
AIM: To assess the effect of gastroduodenal artery (GDA) occlusion prior to selective internal radiation therapy (SIRT) with regards to arterial hepato-intestinal collateralization (HIC). MATERIALS AND METHODS: Six hundred and six patients were scheduled for SIRT between 2006 and 2012 at University Hospital Essen, Germany. Digital subtraction angiography (DSA) followed by administration of 99m-technetium labelled human serum albumin microspheres ((99m)Tc-HSAM) and single-photon emission computed tomography combined with computed tomography (SPECT/CT) was initially performed...
May 2014: Clinical Radiology
https://www.readbyqxmd.com/read/24526453/the-genetics-of-resistance-to-powdery-mildew-in-cultivated-oats-avena-sativa-l-current-status-of-major-genes
#16
Sai L K Hsam, Volker Mohler, Friedrich J Zeller
The genetics of resistance to powdery mildew caused by Blumeria graminis f. sp. avenae of four cultivated oats was studied using monosomic analysis. Cultivar 'Bruno' carries a gene (Pm6) that shows a recessive mode of inheritance and is located on chromosome 10D. Cultivar 'Jumbo' possesses a dominant resistance gene (Pm1) on chromosome 1C. In cultivar 'Rollo', in addition to the gene Pm3 on chromosome 17A, a second dominant resistance gene (Pm8) was identified and assigned to chromosome 4C. In breeding line APR 122, resistance was conditioned by a dominant resistance gene (Pm7) that was allocated to chromosome 13A...
May 2014: Journal of Applied Genetics
https://www.readbyqxmd.com/read/24306693/molecular-characterisation-and-evolution-of-hmw-glutenin-subunit-genes-in-brachypodium-distachyon-l
#17
Saminathan Subburaj, Guanxing Chen, Caixia Han, Dongwen Lv, Xiaohui Li, Friedrich J Zeller, Sai L K Hsam, Yueming Yan
Brachypodium distachyon, a small wild grass within the Pooideae family, is a new model organism for exploring the functional genomics of cereal crops. It was shown to have close relationships to wheat, barley and rice. Here, we describe the molecular characterisation and evolutionary relationships of high molecular weight glutenin subunits (HMW-GS) genes from B. distachyon. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), high performance capillary electrophoresis (HPCE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses demonstrated that there was no HMW-GS expression in the Brachypodium grains due to the silencing of their encoding genes...
February 2014: Journal of Applied Genetics
https://www.readbyqxmd.com/read/24270819/relationships-of-agropyron-intermedium-chromosomes-determined-by-chromosome-pairing-and-alcohol-dehydrogenase-isozymes-in-common-wheat-background
#18
S L Hsam, F J Zeller
The relationships of Agropyron intermedium chromosomes in two wheat-Agropyron addition series were determined. Chromosome pairing behaviour revealed that the alien chromosome in lines TAF-2 and L7 of 'Vilmorin'-A. intermedium set are homologous to the alien chromosomes in lines P and C of the 'Caribo'-A. intermedium set respectively. Localization of alcohol dehydrogenase isozyme genes in 'Vilmorin'-Agropyron addition line L4 and in 'Caribo'-Agropyron line O indicated relationships with wheat chromosomes of homoeologous group 4...
September 1982: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/24270650/genetic-control-of-6-phosphogluconate-dehydrogenase-6-pgd-isozymes-in-cultivated-wheat-and-rye
#19
S L Hsam, F J Zeller, W Huber
The 6-phosphogluconate dehydrogenase (6-PGD) zymogram phenotypes of wheat, rye and their aneuploid derivatives were determined. Two genes involved in the production of 6-PGD isozymes were located on chromosome arms CRL (4 RL) and FRL (6 RL) of "Imperial" rye. On the basis of differential interactions between wheat and rye chromosomes, evidence was obtained that genes located on chromosomes 6 A, 6 BL and 7 BL control 6-PGD isozyme activities in 'Chinese Spring' wheat. The wheat and rye 6-PGD zymogram phenotypes were indicative of homoeologous relationships between rye chromosome 6 RL to wheat chromosomes of group 6, and rye chromosome 4 RL to wheat chromosomes of group 7...
December 1982: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/24248358/false-memories-in-highly-superior-autobiographical-memory-individuals
#20
Lawrence Patihis, Steven J Frenda, Aurora K R LePort, Nicole Petersen, Rebecca M Nichols, Craig E L Stark, James L McGaugh, Elizabeth F Loftus
The recent identification of highly superior autobiographical memory (HSAM) raised the possibility that there may be individuals who are immune to memory distortions. We measured HSAM participants' and age- and sex-matched controls' susceptibility to false memories using several research paradigms. HSAM participants and controls were both susceptible to false recognition of nonpresented critical lure words in an associative word-list task. In a misinformation task, HSAM participants showed higher overall false memory compared with that of controls for details in a photographic slideshow...
December 24, 2013: Proceedings of the National Academy of Sciences of the United States of America
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