Jennifer A Munley, Lauren S Kelly, Gwoncheol Park, Stacey K Drury, Gwendolyn S Gillies, Preston S Coldwell, Kolenkode B Kannan, Letitia E Bible, Philip A Efron, Ravinder Nagpal, Alicia M Mohr
BACKGROUND: Previous preclinical studies have demonstrated sex-specific alterations in the gut microbiome following traumatic injury or sepsis alone; however, the impact of host sex on dysbiosis in the setting of postinjury sepsis acutely is unknown. We hypothesized that multicompartmental injury with subsequent pneumonia would result in host sex-specific dysbiosis. METHODS: Male and proestrus female Sprague-Dawley rats (n = 8/group) were subjected to either polytrauma (PT) (lung contusion, hemorrhagic shock, cecectomy, bifemoral pseudofracture), PT plus 2-hours daily restraint stress (PT/RS), PT with postinjury day 1 pseudomonas aeruginosa pneumonia (PT + PNA), PT/RS with pneumonia (PT/RS + PNA), or naive controls...
March 14, 2024: Journal of Trauma and Acute Care Surgery