Hyeong Bin Park, Jiae An, Kee-Hwa Bae, Seung Hyo Hong, Hwan Joon Park, Seongjun Kim, Chang Woo Lee, Byoung-Doo Lee, Ju Hyoung Baek, Nam Young Kim, Jung Eun Hwang
Cypripedium guttatum is a highly restricted terrestrial orchid that faces increasing endangerment owing to its habitat destruction and illegal collection. Compared to epiphytic orchids, terrestrial orchids such as C. guttatum have harder seed coats and more demanding in vitro germination conditions. This study aimed to develop an effective in vitro propagation system for C. guttatum to aid in its conservation. Seeds from mature capsules were subjected to various conditions, including sterilization using 1% sodium hypochlorite (NaOCl) and different light conditions, culture media, hormones, and organic supplements, to assess germination and early seedling development in vitro...
November 7, 2023: Plants (Basel, Switzerland)