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https://www.readbyqxmd.com/read/29644254/fret-based-stoichiometry-measurements-of-protein-complexes-in-vitro
#1
Francesca Mattiroli, Yajie Gu, Karolin Luger
For a complete understanding of biochemical reactions, information on complex stoichiometry is essential. However, measuring stoichiometry is experimentally challenging. Our lab has developed a FRET-based assay to study protein complex stoichiometry in vitro . This assay, also known as Job plot, is set up as a continuous variation of the molar ratio between the two species, kept at constant total concentration. The FRET (Fluorescence Resonance Energy Transfer) between the two fluorescently-labeled proteins is measured and the stoichiometry is inferred from the sample with highest FRET signal...
February 5, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29628958/neonatal-intensive-care-nursing-curriculum-challenges-based-on-context-input-process-and-product-evaluation-model-a-qualitative-study
#2
Mansoureh Ashghali-Farahani, Fatemeh Ghaffari, Sara-Sadat Hoseini-Esfidarjani, Zahra Hadian, Robabeh Qomi, Helen Dargahi
Background: Weakness of curriculum development in nursing education results in lack of professional skills in graduates. This study was done on master's students in nursing to evaluate challenges of neonatal intensive care nursing curriculum based on context, input, process, and product (CIPP) evaluation model. Materials and Methods: This study was conducted with qualitative approach, which was completed according to the CIPP evaluation model. The study was conducted from May 2014 to April 2015...
March 2018: Iranian Journal of Nursing and Midwifery Research
https://www.readbyqxmd.com/read/29614802/associations-between-job-strain-and-arterial-stiffness-a-large-survey-among-enterprise-employees-from-thailand
#3
Orawan Kaewboonchoo, Grace Sembajwe, Jian Li
As an intermediate endpoint to cardiovascular disease, arterial stiffness has received much attention recently. So far, the research on work stress and arterial stiffness is still sparse and inconsistent, and no investigations on work stress and cardiovascular health among the Thai working population have been reported. Therefore, we conducted an epidemiological study among 2141 Thai enterprise employees (858 men and 1283 women) who were free from any diagnosed cardiovascular disease. Work stress was measured using Karasek's Job Demand-Control model for job strain (a combination of high demand and low control)...
April 2, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29614742/preventing-opioid-use-disorders-among-fishing-industry-workers
#4
Angela Wangari Walter, Cesar Morocho, Lauren King, John Bartlett, Debra Kelsey, Monica DeSousa, Gretchen Biesecker, Laura Punnett
Fishing industry workers are at high risk for work-related musculoskeletal disorders (MSDs) and injuries. Prescription opioids used to treat pain injuries may put these workers at increased risk for developing substance disorders. Using a Community-Based Participatory Research approach, formative research was conducted to inform the eventual development of relevant interventions to prevent and reduce opioid use disorders among fishing industry workers. Qualitative interviews ( n = 21) were conducted to assess: knowledge and attitudes about opioid use disorders; features of fishing work that might affect use and/or access to treatment; and community and organizational capacity for prevention and treatment...
March 31, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29609599/time-motion-study-using-mixed-methods-to-assess-service-delivery-by-frontline-health-workers-from-south-india-methods
#5
Samiksha Singh, Sanjeev Upadhyaya, Pradeep Deshmukh, Amol Dongre, Neha Dwivedi, Deepak Dey, Vijay Kumar
BACKGROUND: In India, amidst the increasing number of health programmes, there are concerns about the performance of frontline health workers (FLHW). We assessed the time utilisation and factors affecting the work of frontline health workers from South India. METHODS: This is a mixed methods study using time and motion (TAM) direct observations and qualitative enquiry among frontline/community health workers. These included 43 female and 6 male multipurpose health workers (namely, auxiliary nurse midwives (ANMs) and male-MPHWs), 12 nutrition and health workers (Anganwadi workers, AWWs) and 53 incentive-based community health workers (accredited social health activists, ASHAs)...
April 2, 2018: Human Resources for Health
https://www.readbyqxmd.com/read/29606374/evaluation-of-bacterial-run-and-tumble-motility-parameters-through-trajectory-analysis
#6
Xiaomeng Liang, Nanxi Lu, Lin-Ching Chang, Thanh H Nguyen, Arash Massoudieh
In this paper, a method for extraction of the behavior parameters of bacterial migration based on the run and tumble conceptual model is described. The methodology is applied to the microscopic images representing the motile movement of flagellated Azotobacter vinelandii. The bacterial cells are considered to change direction during both runs and tumbles as is evident from the movement trajectories. An unsupervised cluster analysis was performed to fractionate each bacterial trajectory into run and tumble segments, and then the distribution of parameters for each mode were extracted by fitting mathematical distributions best representing the data...
March 9, 2018: Journal of Contaminant Hydrology
https://www.readbyqxmd.com/read/29577073/an-assigned-responsibility-system-for-robotic-teleoperation-control
#7
Nicolas Small, Kevin Lee, Graham Mann
This paper proposes an architecture that explores a gap in the spectrum of existing strategies for robot control mode switching in adjustable autonomy. In situations where the environment is reasonably known and/or predictable, pre-planning these control changes could relieve robot operators of the additional task of deciding when and how to switch. Such a strategy provides a clear division of labour between the automation and the human operator(s) before the job even begins, allowing for individual responsibilities to be known ahead of time, limiting confusion and allowing rest breaks to be planned...
2018: International Journal of Intelligent Robotics and Applications
https://www.readbyqxmd.com/read/29574558/defining-the-abdominal-radiologist-based-on-the-current-u-s-job-market
#8
David H Hoffman, Andrew B Rosenkrantz
PURPOSE: The purpose of the study is to characterize current practice patterns of abdominal radiologists based on work descriptions within job postings on numerous national radiology specialty websites. METHODS: Job postings for either "abdominal" or "body" radiologists were searched weekly on five society websites (SAR, SCBT-MR, ARRS, ACR, RSNA) over a 1-year period. Postings were reviewed for various characteristics. RESULTS: Nine hundred and sixteen total ads for 341 unique abdominal radiologist positions were reviewed (34...
March 24, 2018: Abdominal Radiology
https://www.readbyqxmd.com/read/29560255/an-explanatory-model-for-the-concept-of-mental-health-in-iranian-youth
#9
Ahdieh Chinekesh, Seyed Ali Hosseini, Farahnaz Mohammadi, Mohammad Esmael Motlagh, Monir Baradaran Eftekhari, Shirin Djalalinia, Gelayol Ardalan
Background: Mental health is considered as an integral and essential component of overall health. Its determinants and related factors are one of the most important research priorities, especially in adolescents and young people. Using a qualitative approach, the present study aimed to identify factors affecting the mental health of youth in Iran. Methods: In 2017, following content analysis principles, and using semi-structured in-depth interviews, we conducted a qualitative study exploring the opinions of young people about mental health...
2018: F1000Research
https://www.readbyqxmd.com/read/29559817/patterns-and-determinants-of-stress-among-consultant-physicians-working-in-saudi-arabia
#10
Fahad D Alosaimi, Hossam S Alawad, Ayedh K Alamri, Abdullah I Saeed, Khalid A Aljuaydi, Alwaleed S Alotaibi, Khalid M Alotaibi, Eiad A Alfaris
Background: Physicians experience several work-related stressors that have been mounting up in recent decades. This study aimed to examine perceived stress and its risk factors and consequences among consultant physicians in Saudi Arabia. Methods: A cross-sectional study was conducted from November 2014 to March 2015 among physicians who were assigned rank of consultant. The stress level was assessed using perceived stress scale (PSS). Results: A total of 582 consultants participated...
2018: Advances in Medical Education and Practice
https://www.readbyqxmd.com/read/29541556/design-of-a-continuous-blood-pressure-measurement-system-based-on-pulse-wave-and-ecg-signals
#11
Jian-Qiang Li, Rui Li, Zhuang-Zhuang Chen, Gen-Qiang Deng, Huihui Wang, Constandinos X Mavromoustakis, Houbing Song, Zhong Ming
With increasingly fierce competition for jobs, the pressures on people have risen in recent years, leading to lifestyle and diet disorders that result in significantly higher risks of cardiovascular disease. Hypertension is one of the common chronic cardiovascular diseases; however, mainstream blood pressure measurement devices are relatively heavy. When multiple measurements are required, the user experience and the measurement results may be unsatisfactory. In this paper, we describe the design of a signal collection module that collects pulse waves and electrocardiograph (ECG) signals...
2018: IEEE Journal of Translational Engineering in Health and Medicine
https://www.readbyqxmd.com/read/29527350/mcme-project-v-2-0-randomised-controlled-trial-of-a-revised-sms-based-continuing-medical-education-intervention-among-hiv-clinicians-in-vietnam
#12
Christopher J Gill, Ngoc Bao Le, Nafisa Halim, Cao Thi Hue Chi, Viet Ha Nguyen, Rachael Bonawitz, Pham Vu Hoang, Hoang Long Nguyen, Phan Thi Thu Huong, Anna Larson Williams, Ngoc Anh Le, Lora Sabin
Background: Continuing medical education (CME) is indispensable, but costs are a barrier. We tested the effectiveness of a novel mHealth intervention (mCME V.2.0) promoting CME among Vietnamese HIV clinicians. Methods: We enrolled HIV clinicians from three provinces near Hanoi. The 6-month intervention consisted of (1) daily short message service multiple-choice quiz questions, (2) daily linked readings, (3) links to online CME courses and (4) feedback messages describing the performance of the participant relative to the group...
2018: BMJ Global Health
https://www.readbyqxmd.com/read/29525016/-communication-at-the-heart-of-effective-group-work
#13
REVIEW
Gael Bodart, Émeline Hamann, Nina Thieblemont
Nursing students conducted a study into the meaning of group work. Based on the experience they gained from their practice placements, as well as their trip organised as part of their optional learning unit, they scrutinised the conditions favourable to the effective functioning of a working group and the pitfalls to avoid. It equipped them with useful references with which to approach their first job.
March 2018: Revue de L'infirmière
https://www.readbyqxmd.com/read/29522946/12-month-trajectories-of-depressive-symptoms-among-nurses-contribution-of-personality-job-characteristics-coping-and-burnout
#14
Wei Duan-Porter, Daniel Hatch, Jane F Pendergast, Gabriele Freude, Uwe Rose, Hermann Burr, Grit Müller, Peter Martus, Anne Pohrt, Guy Potter
BACKGROUND: Job related factors have been associated with higher risk for developing depression, but past studies lacked full consideration of individual factors such as personality and coping. We sought to evaluate associations of personality, coping, job characteristics, and burnout with 12-month trajectories of depressive symptoms among nursing workers. METHODS: Cohort of nursing workers (N = 281) in a private hospital system, with baseline assessments of personality, job characteristics, and coping...
February 27, 2018: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29520939/a-proposed-clinical-research-support-career-pathway-for-noninvestigators
#15
Sheree Smith, Janice Gullick, Jacqueline Ballard, Lin Perry
AIM: To discuss the international experience of clinical research support for noninvestigator roles and to propose a new pathway for Australia, to promote a sustainable research support workforce capable of delivering high-quality clinical research. BACKGROUND: Noninvestigator research support roles are currently characterized by an ad hoc approach to training, with limited role delineation and perceived professional isolation with implications for study completion rates and participant safety...
March 8, 2018: International Journal of Nursing Practice
https://www.readbyqxmd.com/read/29511953/associations-between-workability-and-patient-reported-physical-psychological-and-social-outcomes-in-breast-cancer-survivors-a-cross-sectional-study
#16
Peh Joo Ho, Mikael Hartman, Sofie A M Gernaat, Alex R Cook, Soo Chin Lee, Leon Hupkens, Helena M Verkooijen
PURPOSE: Workability is of increasing importance especially in Asia given the increasing incidence rates and young age of onset of breast cancer. This study explores the determinants of employment and suboptimal workability. And evaluate the association between workability and patient-reported physical, psychological, and social outcomes. METHODS: In a hospital-based cross-sectional study, 327 breast cancer survivors, < 65 years of age and > 1 year post-diagnosis were recruited...
March 6, 2018: Supportive Care in Cancer: Official Journal of the Multinational Association of Supportive Care in Cancer
https://www.readbyqxmd.com/read/29506513/risk-assessment-of-occupational-exposure-to-heavy-metal-mixtures-a-study-protocol
#17
Fatma Omrane, Imed Gargouri, Moncef Khadhraoui, Boubaker Elleuch, Denis Zmirou-Navier
BACKGROUND: Sfax is a very industrialized city located in the southern region of Tunisia where heavy metals (HMs) pollution is now an established matter of fact. The health of its residents mainly those engaged in industrial metals-based activities is under threat. Indeed, such workers are being exposed to a variety of HMs mixtures, and this exposure has cumulative properties. Whereas current HMs exposure assessment is mainly carried out using direct air monitoring approaches, the present study aims to assess health risks associated with chronic occupational exposure to HMs in industry, using a modeling approach that will be validated later on...
March 5, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29505344/distress-and-worry-as-mediators-in-the-relationship-between-psychosocial-risks-and-upper-body-musculoskeletal-complaints-in-highly-automated-manufacturing
#18
Fiona Wixted, Mark Shevlin, Leonard W O' Sullivan
As a result of changes in manufacturing including an upward trend in automation and the advent of the fourth industrial revolution, the requirement for supervisory monitoring and consequently, cognitive demand has increased in automated manufacturing. The incidence of musculoskeletal disorders has also increased in the manufacturing sector. A model was developed based on survey data to test if distress and worry mediate the relationship between psychosocial factors (job control, cognitive demand, social isolation and skill discretion), stress states and symptoms of upper body musculoskeletal disorders in highly automated manufacturing companies (n=235)...
March 5, 2018: Ergonomics
https://www.readbyqxmd.com/read/29481529/dosimetry-is-key-to-good-epidemiology-workers-at-mallinckrodt-chemical-works-had-seven-different-source-exposures
#19
Elizabeth D Ellis, John D Boice, Ashley P Golden, David J Girardi, Sarah S Cohen, Michael T Mumma, Roy E Shore, Richard W Leggett, George D Kerr
Mallinckrodt Chemical Works was the earliest uranium processing facility in the Manhattan Project, beginning in 1942. Even then, concern existed about possible health effects resulting from exposure to radiation and pitchblende dust. This concern was well founded as the facility processed Belgian Congo pitchblende ore that was up to 60% pure uranium with high U content and up to 100 mg of radium per ton. Workers were exposed to external gamma radiation plus internal radiation from inhalation and ingestion of pitchblende dust (uranium, radium, and silica)...
April 2018: Health Physics
https://www.readbyqxmd.com/read/29471756/age-and-gender-differences-in-the-impact-of-labour-market-transitions-on-subjective-health-in-germany
#20
Stefanie Unger, Anita Tisch, Silke Tophoven
AIMS: Applying a gender- and age group-sensitive approach, we investigated the effect of labour-market transitions (job loss and re-employment) on subjective physical and mental health. METHODS: A combination of the difference-in-differences approach and propensity score matching controls for selectivity and initial health differences. This allowed us to analyse the causal effect of job loss and re-employment on subjective health. We made use of data from the German Panel Study Labour Market and Social Security and combined survey information with administrative records of the Federal Employment Agency for employed and unemployed men and women 31-60 years of age ( n = 2213)...
February 2018: Scandinavian Journal of Public Health
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