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https://www.readbyqxmd.com/read/28931057/challenges-and-advances-for-transcriptome-assembly-in-non-model-species
#1
Arnaud Ungaro, Nicolas Pech, Jean-François Martin, R J Scott McCairns, Jean-Philippe Mévy, Rémi Chappaz, André Gilles
Analyses of high-throughput transcriptome sequences of non-model organisms are based on two main approaches: de novo assembly and genome-guided assembly using mapping to assign reads prior to assembly. Given the limits of mapping reads to a reference when it is highly divergent, as is frequently the case for non-model species, we evaluate whether using blastn would outperform mapping methods for read assignment in such situations (>15% divergence). We demonstrate its high performance by using simulated reads of lengths corresponding to those generated by the most common sequencing platforms, and over a realistic range of genetic divergence (0% to 30% divergence)...
2017: PloS One
https://www.readbyqxmd.com/read/28930240/anti-bacterial-activity-of-phenolic-compounds-against-streptococcus-pyogenes
#2
Sabrina Macé, Lisbeth Truelstrup Hansen, H P Vasantha Rupasinghe
BACKGROUND: Worldwide, Streptococcus pyogenes is the leading cause of bacterial pharyngitis. To reduce the use of antibiotics, antimicrobial phytochemical-containing remedies, which have long been in use in traditional medicine, may provide new approaches for management of streptococcal pharyngitis. The objective of this study was to assess the inhibitory activities of 25 natural phenolic compounds against three strains of S. pyogenes. METHODS: After an initial screening, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the nine most effective phenolic compounds were determined...
May 1, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28930235/current-usage-of-traditional-chinese-medicine-for-breast-cancer-a-narrative-approach-to-the-experiences-of-women-with-breast-cancer-in-australia-a-pilot-study
#3
Dianna Porter, Suzanne Cochrane, Xiaoshu Zhu
Background: The use of Traditional Chinese Medicine (TCM) by breast cancer patients is growing. Few studies have examined the complexity of breast cancer survivors' attitudes, lived experiences, barriers, and perceptions in using TCM as part of their treatment journey. This article examines breast cancer survivors' experiences, perceptions of, and benefits (or not) in using TCM. Methods: Qualitative research, using semi-structured interviews, was the chosen methodology. Results: Participants used TCM as a form of self-help and as a complement, not an alternative, to standard care...
April 21, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28929866/culture-and-food-practices-of-african-american-women-with-type-2-diabetes
#4
Lisa L Sumlin, Sharon A Brown
Purpose The goals of this descriptive ethnographic study were to (1) describe the day-to-day selection, preparation, and consumption of food among African American women (AAW) with type 2 diabetes mellitus (T2DM); (2) identify their typical food selections and consumption practices when dining out at restaurants and at social gatherings (ie, church functions, holidays); (3) highlight the valued behaviors and beliefs that influence these women's food practices; and (4) determine how social interactions influence those food practices...
September 1, 2017: Diabetes Educator
https://www.readbyqxmd.com/read/28929700/extending-the-children%C3%A2-s-health-insurance-program-high-stakes-for-families-and-states
#5
Sara Rosenbaum, Rachel Gunsalus, Sara Rothenberg, Sara Schmucker
Issue: Congress must decide whether to extend federal funding authority for the Children’s Health Insurance Program (CHIP), which ends September 30, 2017. CHIP operates much like Medicaid, providing federal matching payments for state program expenses, although CHIP’s funding rate is higher than the federal rate paid for traditional Medicaid and was further enhanced under the Affordable Care Act. States can use their CHIP funds to expand Medicaid, operate separate CHIP programs, or combine the two approaches...
September 2017: Issue Brief of the Commonwealth Fund
https://www.readbyqxmd.com/read/28929660/-establishment-of-biological-assess-for-quality-control-of-fuzi-based-on-determination-of-premature-ventricular-contractions-in-rats
#6
Zhi-Hao Zhao, Ding-Kun Zhang, Ming-Quan Wu, Chun-Yu Li, Li-Juan Cao, Ping Zhang, Cui-Zhe Liu, Jia-Bo Wang, Xiao-He Xiao
Aconiti Lateralis Radix (Fuzi) is a toxic traditional Chinese medicine with definite efficacy. In order to improve the quality control of its different prepared products and ensure the security in clinic, it is significant to establish a method of quality evaluation related to clinic adverse effects. Aiming at the important biological marker of early cardiac toxicity reaction, there was no method to detect it. In this manuscript, a novel approach for measuring the minimal toxic dose (MTD) of premature ventricular contractions (PVC) poisoning of rats was established...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28929650/-application-of-microscopic-spectroscopy-in-quality-control-of-niuhuang-qingxin-pills
#7
Li-Xing Nie, Ye Zhang, Nan-Ping Zhang, Xiao-Ru Hu, Shuai Kang, Jian-Zhong Hou, Zhong Dai, Shuang-Cheng Ma
Application of microscopic spectroscopy in quality control of Niuhuang Qingxin pills was discussed. First, microscopic characteristics specified by the statutory standard of Niuhuang Qingxin pills were summarized. Then new identification method was established for Dioscoreae Rhizoma, Saigae Tataricae Cornu, Cinnamomi Cortex and Saposhnikoviae Radix. Finally, microscopic spectroscopy was used for test of Dioscoreae Rhizoma's adulterant Dioscoreae Fordii Rhizoma.It was the first time for this technology being applied in adulteration test of Chinese patent medicine...
October 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28929627/strategies-and-challenges-in-identifying-function-for-thousands-of-sorf-encoded-peptides-in-meiosis
#8
Ina Hollerer, Andrea Higdon, Gloria A Brar
Recent genomic analyses have revealed pervasive translation from formerly unrecognized short Open Reading Frames (sORFs) during yeast meiosis. Despite their short length, which has caused these regions to be systematically overlooked by traditional gene annotation approaches, meiotic sORFs share many features with classical genes, implying the potential for similar types of cellular functions. We found that sORF expression accounts for ∼10-20% of the cellular translation capacity in yeast during meiotic differentiation and occurs within well-defined time windows, suggesting the production of relatively abundant peptides with stage-specific meiotic roles from these regions...
September 20, 2017: Proteomics
https://www.readbyqxmd.com/read/28929621/new-integrated-approach-to-percutaneous-mitral-valvuloplasty-combining-wilkins-score-with-commissural-calcium-score-and-commissural-area-ratio
#9
Obul Reddy Gajjala, Rajasekhar Durgaprasad, Vanajakshamma Velam, Sridhar Babu Kayala, Latheef Kasala
BACKGROUND: Present echocardiographic scoring systems for percutaneous mitral valvuloplasty (PMV) have limitations, and no scoring system is significant determinant of procedural outcome. In this study, we assess the relationship between various echocardiographic parameters and scoring systems with immediate outcomes and test an integrated approach by combining Wilkins score with other parameters. METHODS: One hundred two patients who had undergone PMV were included in this prospective observational study...
September 2017: Echocardiography
https://www.readbyqxmd.com/read/28929020/variance-component-testing-for-identifying-differentially-expressed-genes-in-rna-seq-data
#10
Sheng Yang, Fang Shao, Weiwei Duan, Yang Zhao, Feng Chen
RNA sequencing (RNA-Seq) enables the measurement and comparison of gene expression with isoform-level quantification. Differences in the effect of each isoform may make traditional methods, which aggregate isoforms, ineffective. Here, we introduce a variance component-based test that can jointly test multiple isoforms of one gene to identify differentially expressed (DE) genes, especially those with isoforms that have differential effects. We model isoform-level expression data from RNA-Seq using a negative binomial distribution and consider the baseline abundance of isoforms and their effects as two random terms...
2017: PeerJ
https://www.readbyqxmd.com/read/28928708/neural-patterns-of-reorganization-after-intensive-robot-assisted-virtual-reality-therapy-and-repetitive-task-practice-in-patients-with-chronic-stroke
#11
Soha Saleh, Gerard Fluet, Qinyin Qiu, Alma Merians, Sergei V Adamovich, Eugene Tunik
Several approaches to rehabilitation of the hand following a stroke have emerged over the last two decades. These treatments, including repetitive task practice (RTP), robotically assisted rehabilitation and virtual rehabilitation activities, produce improvements in hand function but have yet to reinstate function to pre-stroke levels-which likely depends on developing the therapies to impact cortical reorganization in a manner that favors or supports recovery. Understanding cortical reorganization that underlies the above interventions is therefore critical to inform how such therapies can be utilized and improved and is the focus of the current investigation...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28928706/early-stroke-rehabilitation-of-the-upper-limb-assisted-with-an-electromyography-driven-neuromuscular-electrical-stimulation-robotic-arm
#12
Qiuyang Qian, Xiaoling Hu, Qian Lai, Stephanie C Ng, Yongping Zheng, Waisang Poon
BACKGROUND: Effective poststroke motor rehabilitation depends on repeated limb practice with voluntary efforts. An electromyography (EMG)-driven neuromuscular electrical stimulation (NMES)-robot arm was designed for the multi-joint physical training on the elbow, the wrist, and the fingers. OBJECTIVES: To investigate the training effects of the device-assisted approach on subacute stroke patients and to compare the effects with those achieved by the traditional physical treatments...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28928696/promoting-physical-activity-through-priming-the-content-of-motivation
#13
REVIEW
Tom St Quinton
Non-conscious processes are important in influencing the performance of a number of behaviors, such as physical activity. One way that such processes can be influenced is through priming. Despite this, approaches within health psychology have predominantly focused on reflective processes with a number of psychological theories dedicated to identifying the predictors of intention. In doing so, critical beliefs and thoughts are first identified and then altered within interventions. Such work has shown limited effectiveness, however, with a gap apparent between what one intends to do and what subsequently ensues...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28928663/comparison-of-the-bioactive-components-in-two-seeds-of-ziziphus-species-by-different-analytical-approaches-combined-with-chemometrics
#14
Sheng Guo, Jin-Ao Duan, Yiqun Li, Ruiqing Wang, Hui Yan, Dawei Qian, Yuping Tang, Shulan Su
The Ziziphus species are considered to be the medicine and food dual purposes plants. Among them, the seed of Ziziphus jujuba var. spinosa (ZS) has traditionally been used as an ethnomedicine in Asian countries for thousands years. Owing to the significant benefits for human health, the demand for ZS increased year by year, and the wild resources have become increasingly scarce, which resulted in a shortage of market supply for ZS and product adulteration by substituting ZS with the seeds of Z. mauritiana Lam...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28928656/date-palm-phoenix-dactylifera-fruits-as-a-potential-cardioprotective-agent-the-role-of-circulating-progenitor-cells
#15
Ibrahim A Alhaider, Maged E Mohamed, K K M Ahmed, Arun H S Kumar
Context: Date palms, along with their fruits' dietary consumption, possess enormous medicinal and pharmacological activities manifested in their usage in a variety of ailments in the various traditional systems of medicine. In recent years, the identification of progenitor cells in the adult organ systems has opened an altogether new approach to therapeutics, due to the ability of these cells to repair the damaged cells/tissues. Hence, the concept of developing therapeutics, which can mobilize endogenous progenitor cells, following tissue injury, to enhance tissue repair process is clinically relevant...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28928450/nonreciprocal-reconfigurable-microwave-optomechanical-circuit
#16
N R Bernier, L D Tóth, A Koottandavida, M A Ioannou, D Malz, A Nunnenkamp, A K Feofanov, T J Kippenberg
Nonreciprocal microwave devices are ubiquitous in radar and radio communication and indispensable in the readout chains of superconducting quantum circuits. Since they commonly rely on ferrite materials requiring large magnetic fields that make them bulky and lossy, there has been significant interest in magnetic-field-free on-chip alternatives, such as those recently implemented using the Josephson nonlinearity. Here, we realize reconfigurable nonreciprocal transmission between two microwave modes using purely optomechanical interactions in a superconducting electromechanical circuit...
September 19, 2017: Nature Communications
https://www.readbyqxmd.com/read/28927698/biochemistry-of-mitochondrial-coenzyme-q-biosynthesis
#17
REVIEW
Jonathan A Stefely, David J Pagliarini
Coenzyme Q (CoQ, ubiquinone) is a redox-active lipid produced across all domains of life that functions in electron transport and oxidative phosphorylation and whose deficiency causes human diseases. Yet, CoQ biosynthesis has not been fully defined in any organism. Several proteins with unclear molecular functions facilitate CoQ biosynthesis through unknown means, and multiple steps in the pathway are catalyzed by currently unidentified enzymes. Here we highlight recent progress toward filling these knowledge gaps through both traditional biochemistry and cutting-edge 'omics' approaches...
September 16, 2017: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/28927667/development-and-validation-of-a-tool-to-evaluate-the-evolution-of-clinical-reasoning-in-trauma-using-virtual-patients
#18
David Fleiszer, Michael L Hoover, Nancy Posel, Tarek Razek, Simon Bergman
CONTEXT: Undergraduate medical students at a large academic trauma center are required to manage a series of online virtual trauma patients as a mandatory exercise during their surgical rotation. PURPOSE: Clinical reasoning during undergraduate medical education can be difficult to assess. The purpose of the study was to determine whether we could use components of the students' virtual patient management to measure changes in their clinical reasoning over the course of the clerkship year...
September 16, 2017: Journal of Surgical Education
https://www.readbyqxmd.com/read/28927385/frontline-learning-of-medical-teaching-you-pick-up-as-you-go-through-work-and-practice
#19
W Hartford, L Nimmon, T Stenfors
BACKGROUND: Few medical teachers have received formal teaching education. Along with individual and organizational barriers to participation in teacher training programs, increasing numbers and altered distribution of physicians away from major teaching centers have increased the difficulty of attendance. Furthermore, it is not known if traditional faculty development formats are the optimal learning options given findings from existing studies document both positive and negative outcomes...
September 19, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/28927213/ten-step-minimally-invasive-spine-lumbar-decompression-and-dural-repair-through-tubular-retractors
#20
Mohamed Abdelatif Boukebir, Connor David Berlin, Rodrigo Navarro-Ramirez, Tim Heiland, Karsten Schöller, Cameron Rawanduzy, Sertaç Kirnaz, Ajit Jada, Roger Härtl
BACKGROUND: Minimally invasive spine (MIS) surgery utilizing tubular retractors has become an increasingly popular approach for decompression in the lumbar spine. However, a better understanding of appropriate indications, efficacious surgical techniques, limitations, and complication management is required to effectively teach the procedure and to facilitate the learning curve. OBJECTIVE: To describe our experience and recommendations regarding tubular surgery for lumbar disc herniations, foraminal compression with unilateral radiculopathy, lumbar spinal stenosis, synovial cysts, and dural repair...
April 1, 2017: Operative Neurosurgery (Hagerstown, Md.)
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