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https://www.readbyqxmd.com/read/28325018/imquant-an-image-based-fluorescence-reader-for-quantitative-lateral-flow-immunoassays
#1
Kaushik Basak Chowdhury, Jayaraj Joseph, Jayaraman Kiruthi Vasan, Mohanasankar Sivaprakasam
Fluorescence immunoassays (FIA) have strong potential in enabling rapid quantitative immunological testing at point of care. Existing state of the art instruments employ laser scanning of FIA test kits, which require the use of electromechanical systems, making the instrument bulky, heavy and less reliable in the long term. There is a growing need for an extremely compact and reliable, motion-free, automated reader for accurate quantification of immunoassays. Here, we present ImQuant, an image based FIA reader with integrated monochromatic excitation source, optical filtering and real time image capture, assisted with an intuitive graphical user interface...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324762/chrysanthemum-morphology-photosynthetic-efficiency-and-antioxidant-capacity-are-differentially-modified-by-light-quality
#2
Liang Zheng, Marie-Christine Van Labeke
The effect of light quality on leaf morphology, photosynthetic efficiency and antioxidant capacity of leaves that fully developed under a specific spectrum was investigated in Chrysanthemum cv. Four light treatments were applied at 100μmolm(-2)s(-1) and a photoperiod of 14h using light-emitting diodes, which were 100% red (R), 100% blue (B), 75% red with 25% blue (RB) and white (W), respectively. Intraspecific variation was investigated by studying the response of eight cultivars. Overall, red light significantly decreased the leaf area while the thinnest leaves were observed for W...
March 11, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28324726/facile-synthesis-of-hierarchical-mesoporous-weirds-like-morphological-mno2-thin-films-on-carbon-cloth-for-high-performance-supercapacitor-application
#3
Pragati A Shinde, Vaibhav C Lokhande, Taeksoo Ji, Chandrakant D Lokhande
The mesoporous nanostructured metal oxides have a lot of capabilities to upsurge the energy storing capacity of the supercapacitor. In present work, different nanostructured morphologies of MnO2 have been successfully fabricated on flexible carbon cloth by simple but capable hydrothermal method at different deposition temperatures. The deposition temperature has strong influence on reaction kinetics, which subsequently alters the morphology and electrochemical performance. Among different nanostructured MnO2 thin films, the mesoporous weirds composed thin film obtained at temperature of 453K exhibits excellent physical and electrochemical features for supercapacitor application...
March 3, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28323973/how-to-measure-adipose-tissue-insulin-sensitivity
#4
Esben Søndergaard, Ana Elena Espinosa De Ycaza, Maria Morgan-Bathke, Michael D Jensen
Background: Adipose tissue insulin resistance may be a proximate cause of hepatic and skeletal muscle insulin resistance by releasing excess FFA. However, no consensus exists on how to quantify adipose tissue insulin sensitivity. We compared three methods for measuring adipose tissue insulin sensitivity ranging from the complex multistep pancreatic clamp technique to the simple adipose tissue insulin resistance index (Adipo-IR). Methods: We completed studies of 25 adults with a wide range of insulin sensitivity...
January 31, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323768/use-of-cardiovascular-age-for-assessing-risks-and-benefits-of-menopausal-hormone-therapy
#5
Richard J Santen
The Women's Health Initiative studies and others have suggested that menopausal hormone therapy may enhance the risk of new cardiovascular (CV) events in older women and diminish the development of coronary atherosclerosis in younger women. The underlying mechanisms to explain these findings are encapsulated in the term "Timing Hypothesis." Extensive pathophysiologic studies have provided mechanistic evidence for the dichotomous effects of estrogen on coronary artery vasculature. Early in the atherosclerotic disease process, estrogen exerts protective effects on the endothelium and retards plaque formation...
March 20, 2017: Menopause: the Journal of the North American Menopause Society
https://www.readbyqxmd.com/read/28319868/synthesis-characterization-of-amide-substituted-dexibuprofen-derivatives-and-their-spectral-voltammetric-and-docking-investigations-for-dna-binding-interactions
#6
Nasima Arshad, Muhammad Zafran, Zaman Ashraf, Fouzia Perveen
Three amide derivatives - methyl-2-[2-(4-isobutylphenyl)propanamido]propanoate (Dex-2), methyl 2-[2-(4-isobutylphenyl) propanamido]-3-phenylpropanoate (Dex-3) and methyl 2-[2-(4-isobutylphenyl)-propanamido]-4-methylpentanoate (Dex-4) of dexibuprofen (Dex-1) 2-(4-isobutylphenyl)propanoic acid were synthesized and conformed for structures by physical data and spectral analysis. Further, all the compounds were studied for their binding with ds.DNA through experimental (UV-visible/and fluorescence spectroscopy, cyclic voltammetry) and theoretical (molecular docking) techniques...
March 3, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28319804/are-diabetes-guidelines-truly-evidence-based
#7
REVIEW
Matthew F Bouchonville, Sara Matani, Jason J DuBroff, Robert J DuBroff
The global epidemic of obesity and diabetes underscores the urgency to develop strategies to prevent cardiovascular (CV) disease in this vulnerable population. Clinical guidelines are intended to help the clinician manage these patients, but guidelines are often discordant among professional organizations and not always evidence based. Clinicians must rely upon the best available evidence, and therefore we critically reviewed the evidence behind the American Diabetes Association (ADA) 2016 guidelines on the prevention of CV disease in diabetes...
March 7, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/28319797/non-enolisable-knoevenagel-condensate-appended-schiff-bases-metal-ii-complexes-spectral-characteristics-dna-binding-and-nuclease-activities
#8
Ammavasi Gubendran, Mookkandi Palsamy Kesavan, Srinivasan Ayyanaar, Liviu Mitu, Periyakaruppan Athappan, Jegathalaprathaban Rajesh
New Schiff base complexes [Cu(L(1))Cl] (1), [Ni(L(1))Cl] (2), [Zn(L(1))Cl] (3), and [Fe(L(2))H2OCl] (4) {L(1)=(4E)-3-(2-hydroxybenzylidene)-4-(2-hydroxyphenylimino)pentan-2-one, L(2)=2,2'-(1E,1'E)-(3-(2-hydroxybenzylidene)-pentane-2,4-diylidene)bis(azan-1-yl-1 idene)diphenol} have been synthesized and characterized by elemental analysis, UV-Vis, IR, FAB-mass, EPR, spectral studies and electrochemical studies, the ligands L(1) &L(2) were characterized by (1)H and (13)C NMR spectra. Complex 1 show a visible spectral d-d band near 600nm and display cyclic voltammetric quasireversible response for the Cu(II)/Cu(I) couple vs Ag/AgCl in DMSO...
March 16, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28319274/intraoperative-optical-coherence-tomography-for-soft-tissue-sarcoma-differentiation-and-margin-identification
#9
Kelly J Mesa, Laura E Selmic, Paritosh Pande, Guillermo L Monroy, Jennifer Reagan, Jonathan Samuelson, Elizabeth Driskell, Joanne Li, Marina Marjanovic, Eric J Chaney, Stephen A Boppart
BACKGROUND AND OBJECTIVE: Sarcomas are rare but highly aggressive tumors, and local recurrence after surgical excision can occur in up to 50% cases. Therefore, there is a strong clinical need for accurate tissue differentiation and margin assessment to reduce incomplete resection and local recurrence. The purpose of this study was to investigate the use of optical coherence tomography (OCT) and a novel image texture-based processing algorithm to differentiate sarcoma from muscle and adipose tissue...
March 20, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28319133/effects-of-sudden-walking-perturbations-on-neuromuscular-reflex-activity-and-three-dimensional-motion-of-the-trunk-in-healthy-controls-and-back-pain-symptomatic-subjects
#10
Juliane Mueller, Tilman Engel, Steffen Mueller, Josefine Stoll, Heiner Baur, Frank Mayer
BACKGROUND: Back pain patients (BPP) show delayed muscle onset, increased co-contractions, and variability as response to quasi-static sudden trunk loading in comparison to healthy controls (H). However, it is unclear whether these results can validly be transferred to suddenly applied walking perturbations, an automated but more functional and complex movement pattern. There is an evident need to develop research-based strategies for the rehabilitation of back pain. Therefore, the investigation of differences in trunk stability between H and BPP in functional movements is of primary interest in order to define suitable intervention regimes...
2017: PloS One
https://www.readbyqxmd.com/read/28318236/in-situ-monitoring-potential-dependent-electrochemical-process-by-liquid-nuclear-magnetic-resonance-nmr-spectroelectrochemical-determination-a-proof-of-concept-study
#11
Shuo-Hui Cao, Zu-Rong Ni, Long Huang, Hui-Jun Sun, Biao Tang, Liangjie Lin, Yuqing Huang, Zhi-You Zhou, Shi-Gang Sun, Zhong Chen
We report the design and the performance of a two-chamber thin-layer electrochemical device for in situ potential-dependent liquid nuclear magnetic resonance (NMR) measurement. Liquid NMR spectra, simultaneously recorded with cyclic voltammetry (CV), have been obtained to reveal molecular changes with potentials scanning. As a proof of concept, redox properties of 1,4-benzoquinone based systems have been investigated, and a π dimerization has been identified by combining both in situ and ex situ NMR analyses...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28318215/semaglutide-reduces-cv-events-in-high-risk-patients-with-type-2-diabetes-mellitus
#12
Mark H Ebell
No abstract text is available yet for this article.
March 15, 2017: American Family Physician
https://www.readbyqxmd.com/read/28318211/detrimental-effect-of-tight-glucose-control-on-cv-mortality-persists-over-nine-years
#13
Allen F Shaughnessy
No abstract text is available yet for this article.
March 15, 2017: American Family Physician
https://www.readbyqxmd.com/read/28318108/neonatal-mono-colonization-of-germ-free-mice-with-lactobacillus-casei-enhances-casein-immunogenicity-after-oral-sensitization-to-cow-s-milk
#14
Matieny Aicha Maiga, Stéphanie Morin, Hervé Bernard, Sylvie Rabot, Karine Adel -Patient, Stéphane Hazebrouck
SCOPE: Food allergy is an increasing global health problem and perinatal administration of probiotic bacteria is currently under investigation in order to prevent the development of allergic diseases. Here, we investigated the impact of neonatal mono-colonization of mice with Lactobacillus casei BL23 on an oral sensitization to cow's milk. METHODS AND RESULTS: Mono-colonized (LC) mice were obtained by inoculating L. casei to germ-free (GF) parents. Nine-week-old GF, LC and conventional (CV) mice were orally sensitized to cow's milk with cholera toxin as adjuvant...
March 20, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28317378/scrambled-internal-standard-sis-method-for-high-throughput-protein-quantification-by-maldi-ms-ms
#15
Toshihiro Yoneyama, Sumio Ohtsuki, Masanori Tachikawa, Yasuo Uchida, Tetsuya Terasaki
Matrix-assisted laser desorption ionization (MALDI) would be advantageous for high-throughput MS acquisition, but suffers from low signal reproducibility. The purpose of this study was to establish a reliable MALDI-tandem mass spectrometry (MS/MS)-based high-throughput quantification of tryptic peptides using our newly developed scrambled internal standard (sIS) method. The standard curves obtained with sIS peptides showed good linearity over a wide concentration range (5-1000 fmol/μL) compared to the IS-free method, and the coefficient of variation (CV) of data points at each concentration (5-1000 fmol/μL) was significantly reduced...
March 20, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28317040/intra-individual-variability-of-mycophenolic-acid-concentration-according-to-renal-function-in-liver-transplant-recipients-receiving-mycophenolate-monotherapy
#16
Shin Hwang, Gi-Won Song, Dong-Hwan Jung, Gil-Chun Park, Chul-Soo Ahn, Deok-Bog Moon, Tae-Yong Ha, Ki-Hun Kim, Sung-Gyu Lee
BACKGROUNDS/AIMS: Mycophenolate mofetil (MMF) has wide inter- and intra-individual variability of mycophenolic acid (MPA) after liver transplantation (LT). On this study, we aimed to analyse the intra-individual variability of MPA concentration in stable adult LT recipients receiving MMF monotherapy and develop a method to determine the target level in the situation of wide intra-individual variability. METHODS: This retrospective cross-sectional study included 30 LT recipients...
February 2017: Ann Hepatobiliary Pancreat Surg
https://www.readbyqxmd.com/read/28316073/arrhythmic-effects-of-epac-mediated-ryanodine-receptor-activation-in-langendorff-perfused-murine-hearts-are-associated-with-reduced-conduction-velocity
#17
Mengye Li, Sandeep Hothi, Samantha C Salvage, Kamalan Jeevaratnam, Andrew A Grace, Christopher L-H Huang
Recent papers have attributed arrhythmic substrate in murine RyR2-P2328S hearts to reduced action potential (AP) conduction velocities (CV), reflecting acute functional inhibition and/or reduced expression of sodium channels. We explored for acute effects of direct Epac (exchange protein directly activated by cAMP)-mediated ryanodine receptor-2 (RyR2) activation on arrhythmic substrate and CV. Monophasic action potential recordings demonstrated that initial steady (8-Hz) extrinsic pacing elicited ventricular tachycardia (VT) in 0 of 18 Langendorff-perfused wild-type mouse ventricles before pharmacological intervention...
March 18, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28315504/association-of-triiodothyronine-levels-with-left-ventricular-function-cardiovascular-events-and-mortality-in-hemodialysis-patients
#18
Jianjun Gao, Yongxing Xu, Deyang Kong, Lei Zhou, Huaping Jia, Boran Liang, Zhaoyan Gu
BACKGROUND: Hemodialysis (HD) patients have altered free triiodothyronine (fT3) levels. A low fT3 level is a strong and inverse mortality predictor in HD patients. However, little is known about the relationship between fT3 and left ventricular function in HD patients. METHODS: A total of 128 maintenance HD patients were enrolled in this study. A thyroid function test with blood sampling and echocardiography was conducted. Low-T3 syndrome was defined as fT3 level <3...
February 28, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28315450/phospholipid-oxidation-and-carotenoid-supplementation-in-alzheimer-s-disease-patients
#19
O S Ademowo, H K I Dias, I Milic, A Devitt, R Moran, R Mulcahy, A N Howard, J M Nolan, H R Griffiths
Alzheimer's disease (AD) is a progressive, neurodegenerative disease, characterised by decline of memory, cognitive function and changes in behaviour. Generic markers of lipid peroxidation are increased in AD, therefore reactive oxygen species have been suggested to be involved in the aetiology of cognitive decline. Carotenoids are depleted in AD serum, therefore we have compared serum lipid oxidation between AD and age-matched control subjects before and after carotenoid supplementation. The novel oxidised phospholipid biomarker 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC) was analysed using electrospray ionization tandem mass spectrometry (MS) with multiple reaction monitoring (MRM), 8-isoprostane (IsoP) was measured by ELISA and ferric reducing antioxidant potential (FRAP) was measured by a colorimetric assay...
March 14, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/28315137/sheep-fed-with-banana-leaf-hay-reduce-ruminal-protozoa-population
#20
Cláudio Eduardo Silva Freitas, Eduardo Robson Duarte, Dorismar David Alves, Isabel Martinele, Marta D'Agosto, Franciane Cedrola, Angélica Alves de Moura Freitas, Franklin Delano Dos Santos Soares, Makenzi Beltran
A ciliate protozoa suppression can reduce methane production increasing the energy efficiency utilization by ruminants. The physicochemical characteristics of rumen fluid and the profile of the rumen protozoa populations were evaluated for sheep fed banana leaf hay in replacement of the Cynodon dactylon cv. vaqueiro hay. A total of 30 male sheep were raised in intensive system during 15 days of adaptation and 63 days of experimental period. The animals were distributed in a completely randomized design that included six replicates of five treatments with replacement levels (0, 25, 50, 75, and 100%) of the grass vaquero for the banana leaf hay...
March 17, 2017: Tropical Animal Health and Production
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