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S X Li, J T Wang, Y Jiang, X Wang, W Y Shi
Objective: To evaluate the efficacy of modified deep lamellar keratoplasty (DLKP) combined with antiviral medications for severe herpes necrotizing stromal keratitis. Methods: Retrospective case series study. Modified DLKP was performed in combination with antiviral medications in fifty patients (50 eyes) with severe necrotizing stromal keratitis, which was unresponsive to systemic and topical antiviral treatment for 1 week, at Shandong Eye Hospital. Before surgery, the operated eyes were examined using slit-lamp microscopy...
February 11, 2018: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Wen-Ya Qiu, Li-Bin Zheng, Fei Pan, Bei-Bei Wang, Yu-Feng Yao
BACKGROUND: Reis-Bücklers corneal dystrophy (RBCD) was consistently reported as a corneal dystrophy only affected Bowman's layer and superficial corneal stroma, and superficial keratectomy was a recommendation surgery for treatment in literatures. The study reported new histopathological and ultrastructural findings in RBCD caused by the Arg124Leu mutation of transforming growth factor induced (TGFBI) gene in a four-generation Chinese pedigree. METHODS: Subjects including eight patients and seven unaffected family members received slit-lamp biomicroscopy and photography...
September 2, 2016: BMC Ophthalmology
Dermot O'Sullivan, Michael Henry, Helena Joyce, Naomi Walsh, Edel Mc Auley, Paul Dowling, Niall Swan, Michael Moriarty, Paul Barnham, Martin Clynes, Annemarie Larkin
Development of more effective therapeutic strategies for cancers of high unmet need requires the continued discovery of disease-specific protein targets for therapeutic antibody targeting. In order to identify novel proteins associated with cancer cell invasion/metastasis, we present here an alternative to antibody targeting of cell surface proteins with an established role in invasion; our functional antibody screening approach involves the isolation and selection of MAbs that are primarily screened for their ability to inhibit tumour invasion...
July 2014: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
A P Toner, F McLaughlin, F J Giles, F J Sullivan, E O'Connell, L A Carleton, L Breen, G Dunne, A M Gorman, J D Lewis, S A Glynn
BACKGROUND: Taxanes are routinely used for the treatment of prostate cancer, however the majority of patients eventually develop resistance. We investigated the potential efficacy of EL102, a novel toluidine sulphonamide, in pre-clinical models of prostate cancer. METHODS: The effect of EL102 and/or docetaxel on PC-3, DU145, 22Rv1 and CWR22 prostate cancer cells was assessed using cell viability, cell cycle analysis and PARP cleavage assays. Tubulin polymerisation and immunofluorescence assays were used to assess tubulin dynamics...
October 15, 2013: British Journal of Cancer
Soliman A Al-Kharashi, Majed M Al-Obailan, Mansour Almohaimeed, Abdullah A Al-Torbak
Keratoconus is a disease causing increased steepening of the cornea resulted in irregular astigmatism. Treatment options are Glasses, Hard contact lenses, Cross linking, Intracorneal Segments insertion, Refractive surgery (Gilda et al., 2008), or Keratoplasty. Lamellar Keratoplasty (LKP) can be a better choice to manage cases of moderate and some cases of severe Keratoconus without deep scarring and severe thinning, also in cases of corneal scarring not involving the deeper layers of the cornea. LKP is a corneal graft technique consisting of transplantation of partial-thickness donor tissue, devoid of endothelium, Descemet membrane (DM), and rear stroma into a recipient healthy stromal bed after dissection of pathologic anterior stroma...
October 2009: Saudi Journal of Ophthalmology: Official Journal of the Saudi Ophthalmological Society
Joanne Keenan, Helena Joyce, Sinead Aherne, Shirley O'Dea, Padraig Doolan, Vincent Lynch, Martin Clynes
Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respectively). DLKP-I shows increased apoptosis consistently over all time points tested while DLKP-M appear to slow down metabolically and perhaps delays onset of anoikis by undergoing autophagy. Expression microarray analysis identified pronounced differential expression of Olfactomedin 3 (OLFM3) between the clones...
March 10, 2012: Experimental Cell Research
K Shakya, H J Ruskin, G Kerr, M Crane, J Becker
Data preprocessing in microarray technology is a crucial initial step before data analysis is performed. Many preprocessing methods have been proposed but none has proved to be ideal to date. Frequently, datasets are limited by laboratory constraints so that the need is for guidelines on quality and robustness, to inform further experimentation while data are yet restricted. In this paper, we compared the performance of four popular methods, namely MAS5, Li & Wong pmonly (LWPM), Li & Wong subtractMM (LWMM), and Robust Multichip Average (RMA)...
2010: Advances in Experimental Medicine and Biology
Sandra Roche, Gillian McMahon, Martin Clynes, Robert O'Connor
A highly sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method has been developed to quantify cellular levels of the tyrosine kinase inhibitors (TKIs) dasatinib (Sprycel) and lapatinib (Tykerb, Tyverb). Cellular samples were extracted with a tert-butyl methyl ether:acetonitrile (3:1, v/v):1 M ammonium formate pH 3.5 (8:1, v/v) mixture. Separation was achieved on a Hyperclone BDS C18 (150 mm x 2.0 mm 3 microm) column with isocratic elution using a mobile phase of acetonitirile-10 mM ammonium formate, pH 4 (54:46, v/v), at a flow rate of 0...
December 1, 2009: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Berit Byström, Sergio Carracedo, Anders Behndig, Donald Gullberg, Fatima Pedrosa-Domellöf
PURPOSE: To examine the distribution of the alpha11 integrin chain in the human cornea during fetal development and in normal and diseased adult human corneas. METHODS: Six fetal corneas, 10 to 20 weeks of gestation (wg), and 18 adult corneas including 3 normal, 7 with keratoconus, 5 with pseudophakic bullous keratopathy (PBK), 2 with Fuchs' corneal dystrophy, and 1 with a scar after deep lamellar keratoplasty (DLKP) were processed for immunohistochemistry with specific antibodies against the alpha11 integrin chain; collagen I, IV, and V; and alpha-smooth muscle actin (alpha-SMA)...
November 2009: Investigative Ophthalmology & Visual Science
Brian W Moran, Frankie P Anderson, Aoife Devery, Stephen Cloonan, William E Butler, Sunil Varughese, Sylvia M Draper, Peter T M Kenny
Resveratrol is a potential chemopreventive agent and can be isolated from grape skins and other dietary sources. The Wittig reaction and the decarbonylative Heck reaction were employed to synthesise analogues of this stilbene. Fluorinated derivatives of this stilbene were synthesised maintaining the 3,4',5-substitution pattern. The hydroxyl groups were also replaced by amino groups and the biological activity evaluated. The compounds were assayed on a variety of cell lines, primarily the non-small lung carcinoma cell line DLKP-A...
July 1, 2009: Bioorganic & Medicinal Chemistry
Margarita Simeonova, Galya Ivanova, Venelin Enchev, Nadezhda Markova, Mihail Kamburov, Christo Petkov, Aoife Devery, Robert O'Connor, Dermot Brougham
The design, preparation and characterization of poly(butylcyanoacrylate) nanoparticles as a drug-delivery system for daunorubicin is reported. A range of light scattering [photon correlation spectroscopy (PCS)], spectroscopic [(1)H nuclear magnetic resonance ((1)H NMR), Fourier transform infrared (FTIR), chromatographic [gel permeation chromatography (GPC)] and quantum chemical techniques have been employed for the physicochemical characterization of drug-loaded nanoparticles and to clarify the mechanisms of drug immobilization in the polymer matrix...
July 2009: Acta Biomaterialia
Joanne Keenan, Lisa Murphy, Michael Henry, Paula Meleady, Martin Clynes
Alterations in protein expression associated with adriamycin resistance in a panel of variants derived from the poorly differentiated squamous cell lung carcinoma DLKP were investigated using 2-D DIGE. Of the 80 proteins identified as being differentially expressed, 32 correlated to adriamycin resistance. Twenty-four proteins showed positive correlations with drug resistance, 11 correlated directly with increase in the resistance (including NDPK, RPA2, CCT2, HSP70 and Annexin A1) while 13 proteins (including HNRP K and H1, aldehyde dehydrogenase (ALDH), stomatin and CCT3) increased to a similar level in all drug-resistant variants...
March 2009: Proteomics
Yu-Feng Yao
In recent years, deep lamellar keratoplasty (DLKP) has become an alternative surgical approach successfully applied to the treatment of opaque cornea with normal endothelial function. However, techniques for performing DLKP are not standardized and have not been perfected compared with penetrating keratoplasty. We developed a technique of stromal hooking and viscoelastic detaching to create a full-bed exposure of Descemet membrane (DM) for DLKP grafting. The surgical procedures include trephining the recipient stroma, exposure of DM by stromal hooking process using a forceps tooth as hook, detaching stroma from DM by injecting viscoelastic between the 2 layers, removing whole stroma around the trephined margin to obtain full-bed exposure of DM, and grafting cryopreserved donor button...
September 2008: Cornea
Jia-Qi Chen, Jin Yuan
Corneal transplantation is an effective procedure for the treatment of opaque cornea, but the application of lamellar keratoplasty is limited because its optical results are not as good as penetrating corneal transplantation. Recently, with the appearance of new surgical procedures, deep lamellar keratoplasty (DLKP) and deep lamellar endothelial keratoplasty (DLEK), clinicians should re-evaluate the value of lamellar keratoplasty. This article discusses the designs, the indications and the characteristics of these new surgical procedures, analyzes the problems existed in China, and looks forward to the future about the development of lamellar keratoplasty...
February 2008: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Piero Ceruti, Roberta Morbio, Michele Marraffa, Giorgio Marchini
PURPOSE: To compare the measurements of intraocular pressure (IOP) with dynamic contour tonometry (DCT) and Goldmann applanation tonometry (GAT) in eyes with corneal graft and to evaluate the influence of corneal thickness (CCT), corneal curvature (CC), and astigmatism on these methods. DESIGN: Prospective, observational cross-sectional study. METHODS: Eighteen eyes of 18 patients after penetrating keratoplasty (PKP) and 14 eyes of 14 patients after deep lamellar keratoplasty (DLKP) underwent IOP evaluation with DCT and GAT, and measurements of CCT, CC, and astigmatism...
February 2008: American Journal of Ophthalmology
Jian-jiang Xu, Qi-hua Le, Xing-huai Sun, Chao-ran Zhang, Yan Wang, Jia-xu Hong
OBJECTIVE: To investigate the therapeutic outcomes of deep lamellar keratoplasty (DLKP) and penetrating keratoplasty (PKP) for keratoconus. METHODS: Retrospective review of 29 eyes of 29 keratoconus patients, who underwent surgery in Eye & ENT Hospital of Fudan University from April in 2003 to April in 2006. Eleven eyes underwent DLKP and the rest had PKP. Pre- and post-operative visual acuity, astigmatism, complications and post-operative graft status were assessed...
July 2007: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
Tetsuya Kawakita, Motoko Kawashima, Yoshiyuki Satake, Seika Den, Machiko Tomita, Jun Shimazaki
Penetrating keratoplasty (PKP) has long been the standard procedure for treating corneal opacification. Recent advances in corneal surgery have enabled us to perform component surgery of the cornea, replacing necessary cells and tissue only instead of changing the entire layer of the cornea. Corneal components can be transplanted as lamellar sections of donor cornea or as ex vivo expanded cell sheets with or without biologic carriers. Transplantation of cultivated epithelial sheets expanded from limbal epithelium or oral mucosal epithelium, deep lamellar keratoplasty (DLKP), and deep lamellar endothelial keratoplasty (DLEK) are already in clinical application...
October 2007: Cornea
Naoko Taenaka, Masahiko Fukuda, Tsuyoshi Hibino, Yoko Kato, Eiko Arimura, Yasuo Ishii, Yoshikazu Shimomura
PURPOSE: To report the efficacy of phototherapeutic keratectomy (PTK) and deep lamellar keratoplasty (DLKP) in the treatment of Acanthamoeba keratitis. METHODS: Two patients with delayed diagnosis of Acanthamoeba keratitis failed to respond to multiple systemic and topic antiamoebic agents supplemented by twice-weekly corneal scraping. Three weeks into such therapy, one developed a ring-shaped subepithelial infiltration and PTK was performed. The other was treated with DLKP for progressive keratitis that had invaded the midstromal layer after 50 days of medical therapy...
August 2007: Cornea
L Murphy, M Clynes, J Keenan
BACKGROUND: Mitoxantrone resistance has been related to the expression of a drug efflux pump breast cancer resistance pump (BCRP) but little is known of the intracellular protein changes. In this work, differential protein expression in a squamous lung carcinoma cell line, DLKP, and its mitoxantrone-resistant variant (DLKP-Mitox) was investigated to elucidate other changes associated with mitoxantrone resistance. MATERIALS AND METHODS: Differential protein expression between DLKP and DLKP-Mitox was investigated using 2D-DIGE technology...
May 2007: Anticancer Research
Tetsuya Kawakita, Tamaki Sumi, Murat Dogru, Kazuo Tsubota, Jun Shimazaki
PURPOSE: To report amniotic membrane (AM) transplantation in a patient with wound dehiscence 5 months after deep lamellar keratoplasty (DLKP) METHODS: The patient was an 84-year-old Japanese man who had undergone right DLKP 5 months earlier for central corneal scarring due to recurrent stromal herpetic keratitis. He developed wound dehiscence with corneal stromal melting due to recurrence of stromal herpes in both the donor and recipient sites. "AM roll-in filling technique" and AM patching were performed...
2007: Journal of Medical Case Reports
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