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NAD+ AND NQO1

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https://www.readbyqxmd.com/read/28628905/a-ros-mediated-mitochondrial-pathway-and-nrf2-pathway-activation-are-involved-in-bde-47-induced-apoptosis-in-neuro-2a-cells
#1
Hongmei Chen, Xuexi Tang, Bin Zhou, Zhongyuan Zhou, Ningning Xu, You Wang
Our previous study showed that 2,2'-,4,4'-tetrabromodiphenyl ether (BDE-47) is cytotoxic and induces apoptosis in Neuro-2a cells. In the present study, we aimed to investigate whether nuclear factor (erythroid-derived 2)-like 2 (Nrf2), an antioxidant transcriptional regulator of oxidative stress and apoptosis, is involved in this process. The results of toxicological experiments showed that BDE-47 decreased the cellular mitochondrial membrane potential (MMP) and increased cytochrome c release to the cytoplasm, followed by an increase in intracellular caspase-9 and caspase-3 activity, suggesting that a mitochondrial pathway was involved in the apoptotic process...
June 3, 2017: Chemosphere
https://www.readbyqxmd.com/read/28626497/hepatoprotective-effect-of-polyphenol-enriched-fraction-from-folium-microcos-on-oxidative-stress-and-apoptosis-in-acetaminophen-induced-liver-injury-in-mice
#2
Hongtan Wu, Gang Zhang, Lisen Huang, Haiyue Pang, Na Zhang, Yupei Chen, Gueyhorng Wang
Folium Microcos (FM), the leaves of Microcos paniculata L., shows various biological functions including antioxidant activity and α-glucosidase inhibitory effect. However, its therapeutic potential in acute liver injury is still unknown. This study investigated the hepatoprotective effect and underlying mechanisms of the polyphenol-enriched fraction (FMF) from Folium Microcos. FMF exhibited strong free radical scavenging activities and prevented HepG2/Hepa1-6 cells from hydrogen peroxide- (H2O2-) induced ROS production and apoptosis in vitro...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28620296/testosterone-propionate-exacerbates-the-deficits-of-nigrostriatal-dopaminergic-system-and-downregulates-nrf2-expression-in-reserpine-treated-aged-male-rats
#3
Rui Cui, Yunxiao Kang, Li Wang, Shuangcheng Li, Xiaoming Ji, Wensheng Yan, Guoliang Zhang, Huixian Cui, Geming Shi
There is a controversy over the effects of testosterone supplements on dopaminergic function. Both neuroprotective and toxic effects of testosterone supplements are reported. The status of oxidative stress seems to explain the neuroprotective or toxic properties of testosterone. To determine the efficacy of testosterone supplements in different status of oxidative stress, the present studies analyzed the dopamine (DA)-related behaviors and neurochemical indices, as well as markers of nigrostriatal dopaminergic (NSDA) system in reserpine-treated aged male rats followed by testosterone propionate (TP) supplements...
2017: Frontiers in Aging Neuroscience
https://www.readbyqxmd.com/read/28604588/docosahexaenoic-acid-induces-expression-of-heme-oxygenase-1-and-nad-p-h-quinone-oxidoreductase-through-activation-of-nrf2-in-human-mammary-epithelial-cells
#4
Hye-Yoon Bang, Sin-Aye Park, Soma Saeidi, Hye-Kyung Na, Young-Joon Surh
Docosahexaenoic acid (DHA), an ω-3 fatty acid abundant in fish oils, has diverse health beneficial effects, such as anti-oxidative, anti-inflammatory, neuroprotective, and chemopreventive activities. In this study, we found that DHA induced expression of two representative antioxidant/cytoprotective enzymes, heme oxygenase-1 (HO-1) and NAD(P)H:quinone oxidoreductase (NQO1), in human mammary epithealial (MCF-10A) cells. DHA-induced upregulation of these enzymes was accompanied by enhanced translocation of the redox-sensitive transcription factor Nrf2 into the nucleus and its binding to antioxidant response element...
June 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28599455/downregulation-of-nad-p-h-quinone-oxidoreductase-1-inhibits-proliferation-cell-cycle-and-migration-of-cholangiocarcinoma-cells
#5
Siriwoot Butsri, Veerapol Kukongviriyapan, Laddawan Senggunprai, Sarinya Kongpetch, Ponsilp Zeekpudsa, Auemduan Prawan
We previously reported that upregulation of NAD(P)H:quinone oxidoreductase 1 (NQO1) in cholangiocarcinoma (CCA; a fatal bile duct cancer) was associated with poor prognosis. It was also demonstrated that the suppression of NQO1 was able to enhance the chemosensitivity of CCA cells. In the present study, in order to elucidate the biological role of NQO1 in CCA, the effects of small interfering RNA (siRNA)-mediated knockdown of NQO1 on cell proliferation, cell cycle and migration were determined in KKU-100 CCA cells, which notably expressed NQO1...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28598994/asarone-from-acori-tatarinowii-rhizome-prevents-oxidative-stress-induced-cell-injury-in-cultured-astrocytes-a-signaling-triggered-by-akt-activation
#6
Kelly Y C Lam, Ping Yao, Huaiyou Wang, Ran Duan, Tina T X Dong, Karl W K Tsim
Acori Tatarinowii Rhizome (ATR; the dried rhizome of Acori tatarinowii Schott) is a well-known herb being used for mental disorder in China and Asia. Volatile oil is considered as the active ingredient of ATR, and asarones account for more than 90% of total volatile oil. Here, the protective effects of ATR oil and asarones, both α-asarone and β-asarone, were probed in cultured rat astrocytes. The cyto-protective effect of ATR oil and asarones against tBHP-induced astrocyte injury was revealed, and additionally ATR oil and asarones reduced the tBHP-induced intracellular reactive oxygen species (ROS) accumulation...
2017: PloS One
https://www.readbyqxmd.com/read/28592850/nad-augmentation-ameliorates-acute-pancreatitis-through-regulation-of-inflammasome-signalling
#7
AiHua Shen, Hyung-Jin Kim, Gi-Su Oh, Su-Bin Lee, Seung Hoon Lee, Arpana Pandit, Dipendra Khadka, Seong-Kyu Choe, Sung Chul Kwak, Sei-Hoon Yang, Eun-Young Cho, Hyun-Seok Kim, Hail Kim, Raekil Park, Tae Hwan Kwak, Hong-Seob So
Acute pancreatitis (AP) is a complicated disease without specific drug therapy. The cofactor nicotinamide adenine dinucleotide (NAD(+)) is an important regulator of cellular metabolism and homeostasis. However, it remains unclear whether modulation of NAD(+) levels has an impact on caerulein-induced AP. Therefore, in this study, we investigated the effect of increased cellular NAD(+) levels on caerulein-induced AP. We demonstrated for the first time that the activities and expression of SIRT1 were suppressed by reduction of intracellular NAD(+) levels and the p53-microRNA-34a pathway in caerulein-induced AP...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28586911/serpina3k-ameliorates-the-corneal-oxidative-injury-induced-by-4-hydroxynonenal
#8
Xiling Zheng, Huixia Cui, Yuanyuan Yin, Yajing Zhang, Rongrong Zong, Xiaorui Bao, Jian-Xing Ma, Zuguo Liu, Yueping Zhou
Purpose: We previously demonstrated that SERPINA3K has anti-inflammatory, antiangiogenic, and antioxidant effects in corneas. Here we further investigated the effects of SERPINA3K on the corneal oxidant injury setting recently developed and induced by 4-hydroxynonenal (4-HNE). Methods: We applied the 4-HNE-induced corneal oxidant stress in cultured human corneal epithelial (HCE) cells in vitro and to the cornea of rats in vivo. The following experiments were conducted: cell counting kit 8 assay to detect cell viability; quantitative real-time PCR assay; Western blotting and immunofluorescent staining to measure gene expressions or protein levels of key reactive oxygen species (ROS)-associated factors (3-nitrotyrosine [3-NT]; nicotinamide adenine dinucleotide phosphate [NADPH]-oxidase 4 [NOX4]; superoxide dismutase [SOD]); catalase and nuclear factor [erythroid-derived 2]-like 2 [NRF2]); as well as main factors of the Wnt/β-catenin signaling pathway (p-LRP6, β-catenin and transcription factor 4 [TCF4]); histologic staining; and TUNEL staining to examine sections of rat corneas...
June 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28584577/antioxidant-mechanism-of-black-garlic-extract-involving-nuclear-factor-erythroid-2-like-factor-2-pathway
#9
Ae Wha Ha, Woo Kyoung Kim
BACKGROUN/OBJECTIVES: Although studies have revealed that black garlic is a potent antioxidant, its antioxidant mechanism remains unclear. The objective of this study was to determine black garlic's antioxidant activities and possible antioxidant mechanisms related to nuclear factor erythroid 2-like factor 2 (Nrf2)-Keap1 complex. METHODS/MATERIALS: After four weeks of feeding rats with a normal fat diet (NF), a high-fat diet (HF), a high-fat diet with 0.5% black garlic extract (HF+BGE 0...
June 2017: Nutrition Research and Practice
https://www.readbyqxmd.com/read/28558932/bama-miniature-pigs-liver-possess-great-heat-tolerance-through-upregulation-of-nrf2-mediated-antioxidative-enzymes
#10
Jiaqin Chen, Fei Wang, Xin Zhou, Yun Cao, Yansen Li, Chunmei Li
The liver is one of the most crucial organs affected by high ambient temperature. Bama miniature pig show a heat tolerance in hot summer months. However, the physiological condition of liver under high ambient temperature has not been well elucidated in Bama miniature pig. Here we performed an experiment to investigate the effects of high ambient temperature on liver function, redox status and Nrf2 antioxidant pathway in Bama miniature pigs. Twelve pigs were randomly divided into two groups and separately exposed to the neutral temperature (NT, 25°C) and high temperature (HT, 40°C) for 8 days...
July 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28550120/hiv-1-decreases-nrf2-are-activity-and-phagocytic-function-in-alveolar-macrophages
#11
Bashar S Staitieh, Lingmei Ding, Wendy A Neveu, Paul Spearman, David M Guidot, Xian Fan
Respiratory complications occur frequently in individuals living with human immunodeficiency-1 virus (HIV) infection, and there is evidence that HIV-related oxidative stress impairs alveolar macrophage immune function. We hypothesized that nuclear factor (erythroid-derived 2)-like 2 (Nrf2), a master transcription factor that activates the antioxidant response element (ARE) and regulates antioxidant defenses, has an important role in alveolar macrophage (AMs) immune dysfunction in individuals with HIV infections...
May 26, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28529598/biological-interaction-of-cigarette-smoking-on-the-association-between-genetic-polymorphisms-involved-in-inflammation-and-the-risk-of-lung-cancer-a-case-control-study-in-japan
#12
Yuzo Yamamoto, Chikako Kiyohara, Saiko Suetsugu-Ogata, Naoki Hamada, Yoichi Nakanishi
Chronic inflammation serves an important role in lung carcinogenesis, thus genetic polymorphisms involved in this pathway may affect the risk of lung cancer. The present case-control study focused on the association between lung cancer risk and genetic polymorphisms involved in inflammatory pathways. The study comprised 462 lung cancer cases and 379 controls from Japan. The roles of interleukin 8 (IL8) rs4073, nuclear factor kappa B (NFκB) rs28362491, cytochrome b-245, alpha polypeptide (CYBA) rs4673, NAD(P) H dehydrogenase, quinone 1 (NQO1) rs1800566, nitric oxide synthase 2 and inducible (NOS2) rs2297518 polymorphisms in lung carcinogenesis were investigated...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28523535/self-protection-against-triptolide-induced-toxicity-in-human-hepatic-cells-via-nrf2-are-nqo1-pathway
#13
Ling-Ling Zhou, Cong Zhou, Xiao-Wen Liang, Zhe Feng, Zhang-Pu Liu, Hao-Lu Wang, Xue-Ping Zhou
OBJECTIVE: To find the signaling pathway of triptolide (TP)-induced liver injury and to reveal whether NF-E2-related factor 2 (Nrf2) plays an important role in cellular self-protection. METHODS: The L-02 and HepG2 cells were cultured and treated with various concentrations of TP. The cell viability was observed, and the cell medium was collected for detecting the aspartate aminotransferase (ALT), alanine aminotransferase (AST), lactate dehydrogenase (LDH), superoxide dismutase (SOD) and L-glutathione production (GSH) levels...
May 18, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28522909/in-vitro-and-in-vivo-antioxidative-and-hepatoprotective-activity-of-aqueous-extract-of-cortex-dictamni
#14
Lin Li, Yun-Feng Zhou, Yan-Lin Li, Li-Li Wang, Hiderori Arai, Yang Xu
AIM: To investigate the antioxidant and hepatoprotective effects of Cortex Dictamni aqueous extract (CDAE) in carbon tetrachloride (CCl4)-induced liver damage in rats. METHODS: The in vitro antioxidant effect of CDAE was investigated using α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), β-carotene bleaching, reducing power, and thiobarbituric acid reactive substance assays. A linoleic acid system, including ferric thiocyanate (FTC) and thiobarbituric acid (TBA) assays, was used to evaluate the inhibition of lipid peroxidation...
April 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28513573/nrf2-inhibits-periodontal-ligament-stem-cell-apoptosis-under-excessive-oxidative-stress
#15
Yanli Liu, Hongxu Yang, Yi Wen, Bingyi Li, Yinhua Zhao, Jing Xing, Min Zhang, Yongjin Chen
The present study aimed to analyze novel mechanisms underlying Nrf2-mediated anti-apoptosis in periodontal ligament stem cells (PDLSCs) in the periodontitis oxidative microenvironment. We created an oxidative stress model with H₂O₂-treated PDLSCs. We used real-time PCR, Western blotting, TUNEL staining, fluorogenic assay and transfer genetics to confirm the degree of oxidative stress and apoptosis as well as the function of nuclear factor-erythroid 2-related factor 2 (Nrf2). We demonstrated that with upregulated levels of reactive oxygen species (ROS) and malondialdehyde (MDA), the effect of oxidative stress was obvious under H₂O₂ treatment...
May 17, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28503686/mesoporous-nanocarriers-with-a-stimulus-responsive-cyclodextrin-gatekeeper-for-targeting-tumor-hypoxia
#16
Jeonghun Lee, Eun-Taex Oh, Haerry Yoon, Chan Woo Kim, Yeji Han, Jaehun Song, Hyunil Jang, Heon Joo Park, Chulhee Kim
Tissue hypoxia developed in most malignant tumors makes a significant difference to normal tissues in the reduction potential and the activity of various bioreductive enzymes. Given the superior enzymatic activity of NAD(P)H:quinone oxidoreductase 1 (NQO1, a cytosolic reductase up-regulated in many human cancers) in hypoxia relative to that in normoxia, NQO1 has great potential for targeting hypoxic tumor cells. In the present report, the core concept of hypoxic NQO1-responsive mesoporous silica nanoparticles (MSNs) is based on the reasoning that the superior enzymatic activity of NQO1 within hypoxic cancer cells can be utilized as a key stimulus for the selective cleavage of an azobenzene stalk triggering the on-off gatekeeping for controlled release of guest drugs...
May 25, 2017: Nanoscale
https://www.readbyqxmd.com/read/28498397/dihydroartemisinin-inhibits-the-viability-of-cervical-cancer-cells-by-upregulating-caveolin%C3%A2-1-and-mitochondrial-carrier-homolog%C3%A2-2-involvement-of-p53-activation-and-nad-p-h-quinone-oxidoreductase%C3%A2-1-downregulation
#17
Ting Zhang, Yuan Hu, Ting Wang, Peiling Cai
Dihydroartemisinin (DHA) has been shown to inhibit the viability of various cancer cells. Previous studies have revealed that the mechanisms involved in the inhibitory effects of DHA are based on theactivation of p53 and the mitochondrial-related cell death pathway. However, the exact association between upstream signaling and the activation of cell death pathway remains unclear. In this study, we found that DHA treatment induced the upregulation of caveolin 1 (Cav1) and mitochondrial carrier homolog 2 (MTCH2) in HeLa cells, and this was associated with the DHA-induced inhibition of cell viability and DHA-induced apoptosis...
May 9, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28487966/oligomeric-proanthocyanidins-protects-a549-cells-against-h2o2-induced-oxidative-stress-via-the-nrf2-are-pathway
#18
Chao Sun, Weiguo Jin, Hongcan Shi
Oxidative signaling and oxidative stress contribute to aging, cancer and diseases resulting from lung fibrosis. In this study, we explored the anti-oxidative potential of oligomeric proanthocyanidins (OPCs), natural flavonoid compounds. We examined the protective effects of OPCs against hydrogen peroxide (H2O2)-induced oxidative stress in non-small cell lung cancer cells (A549). We demonstrated that OPC markedly attenuated H2O2-induced A549 cell viability, as shown by by 3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyl-tetrazolium bromide (MTT) assay...
June 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28456064/effects-of-diclofenac-on-the-expression-of-nrf2-and-its-downstream-target-genes-in-mosquito-fish-gambusia-affinis
#19
Shuang Bao, Xiangping Nie, Ruikang Ou, Chao Wang, Peijia Ku, Kaibing Li
Diclofenac (DCF) is one of widely used non-steroidal anti-inflammatory drugs. Recently, this drug has been universally detected in aquatic environment. However, its potential adverse effects and oxidative stress toxic mechanisms on fish remain unclear. In the present study, we first cloned the crucial partial sequences of some key oxidative stress related genes, which include NF-E2-related factor 2 (Nrf2), NAD(P)H: quinoneoxidoreductase (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC), Cu-Zn superoxide dismutase (SOD2), catalase (CAT), alpha-glutathione S-transferase (GSTA), and UDP-glucuronosyltransferases (UGT) in mosquito fish (Gambusia affinis)...
April 20, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28455582/glutathione-s-transferases-deletions-may-act-as-prognosis-and-therapeutic-markers-in-breast-cancer
#20
Clodoaldo Zago Campos, Roberta Losi Guembarovski, Carlos Eduardo Coral de Oliveira, Bruna Karina Banin Hirata, Glauco Akelinghton Freire Vitiello, Flávia Luísa Dias, Carlos Hiroji Hiroki, Maria Angelica Ehara Watanabe, Tânia Longo Mazzuco
Breast cancer (BC) is the main worldwide neoplasia in women. The metabolic balance between xenobiotic absorption and elimination rates plays an important role in preventing DNA damage and, consequently, tumor development. The glutathione S-transferases (GSTs), such as GSTM1 and GSTT1, and the NAD(P)H quinone oxidoreductase are important enzymes involved in phase II detoxification reactions. Deletions in GSTM1 and GSTT1, and single-nucleotide polymorphism (SNP) in NQO1 (rs1800655) have been investigated in cancer context, revealing conflicting results...
April 28, 2017: Clinical and Experimental Medicine
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