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NAD+ AND NQO1

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https://www.readbyqxmd.com/read/29458270/the-involvement-of-nrf2-antioxidant-signaling-pathway-in-the-protection-of-monocrotaline-induced-hepatic-sinusoidal-obstruction-syndrome-in-rats-by-catechin-hydrate
#1
Xiaoqi Jing, Jiaqi Zhang, Zhenlin Huang, Yuchen Sheng, Lili Ji
Hepatic sinusoidal obstruction syndrome (HSOS) is a rare and life-threatening liver disease. (+)-catechin is a natural dietary flavonol with high antioxidant capacity. This study aims to investigate the involvement of nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant signaling pathway in the protection of (+)-catechin hydrate (CAT) against monocrotaline (MCT)-induced HSOS. Results of serum alanine/aspartate aminotransferases (ALT/AST) activities, total bilirubin (TBil) and bile acids (TBA) amounts, liver histological observation, scanning electron microscope evaluation and hepatic metalloproteinase-9 (MMP-9) expression all demonstrated the protection of CAT against MCT-induced HSOS in rats...
February 20, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29453911/induction-of-xenobiotic-metabolizing-enzymes-in-hepatocytes-by-beta-naphthoflavone-time-dependent-changes-in-activities-protein-and-mrna-levels
#2
Kateřina Lněničková, Lenka Skálová, Lucie Stuchlíková, Barbora Szotáková, Petra Matoušková
In the present study, time-dependency of the induction effect of a selective inducer on the activity, protein and mRNA levels of cytochromes P450 1A1/2 (CYP1A1/2), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferases (GSTA), in primary culture of rat hepatocytes was tested and evaluated. To show the differences in responses of tested enzymes, the common aryl hydrocarbon receptor (AhR) ligand agonist, beta-naphthoflavone (BNF), was used. Induction of CYP1A1/2 by BNF was detected at all time intervals and at all levels (i...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29434949/carnosic-acid-an-inducer-of-nad-p-h-quinone-oxidoreductase-1-enhances-the-cytotoxicity-of-%C3%AE-lapachone-in-melanoma-cell-lines
#3
Nobuyuki Arakawa, Ayaka Okubo, Shinji Yasuhira, Kazuhiro Takahashi, Hiroo Amano, Toshihide Akasaka, Tomoyuki Masuda, Masahiko Shibazaki, Chihaya Maesawa
NAD(P)H quinone oxidoreductase 1 (NQO1)-dependent antitumor drugs such as β-lapachone (β-lap) are attractive candidates for cancer chemotherapy because several tumors exhibit higher expression of NQO1 than adjacent tissues. Although the association between NQO1 and β-lap has been elucidated, the effects of a NQO1-inducer and β-lap used in combination remain to be clarified. It has previously been reported that melanoma cell lines have detectable levels of NQO1 expression and are sensitive to NQO1-dependent drugs such as 17-allylamino-17-demethoxygeldanamycin...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29433360/ampk-nrf2-signaling-is-involved-in-the-anti-neuroinflammatory-action-of-petatewalide-b-from-petasites-japonicus-against-lipopolysaccharides-in-microglia
#4
Sun Young Park, Min Hyun Choi, Mei Li, Ke Li, Geuntae Park, Young-Whan Choi
OBJECTIVES: Abnormal microglia secrete neuroinflammatory factors that play a pivotal role in neurodegenerative-disorder development. Thus, regulating abnormal microglia-activation could be a promising therapeutic strategy. The purposes of this study included investigating the effect of Petatewalide B on lipopolysaccharide- (LPS-) stimulated microglia and exploring the role of the AMPK/Nrf2- (adenosine monophosphate-activated protein kinase/nuclear factor erythroid 2-related factor 2) signaling pathway in the anti-neuroinflammatory function of Petatewalide B...
February 12, 2018: Immunopharmacology and Immunotoxicology
https://www.readbyqxmd.com/read/29414880/hematological-abnormality-oxidative-stress-and-genotoxicity-induction-in-the-greenhouse-pesticide-sprayers-investigating-the-role-of-nqo1-gene-polymorphism
#5
Nahid Ahmadi, Ali Mandegary, Akram Jamshidzadeh, Mohaddeseh Mohammadi-Sardoo, Mohammadreza Mohammadi-Sardo, Elham Salari, Leyla Pourgholi
The widespread use of pesticides in agriculture represents a threat to the human populations exposed to them. In this cross-sectional study, the hematological and biochemical parameters, plasma cholinesterase (PChE) activity, oxidative stress, genotoxicity, and NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism were measured in 100 greenhouse workers occupationally exposed to pesticide mixture and 104 normal healthy controls. There was a decrease in erythrocytes (5.45%, p = 0.026) and hemoglobin (3...
February 7, 2018: Toxics
https://www.readbyqxmd.com/read/29402993/partial-involvement-of-nrf2-in-skeletal-muscle-mitohormesis-as-an-adaptive-response-to-mitochondrial-uncoupling
#6
Verena Coleman, Piangkwan Sa-Nguanmoo, Jeannette Koenig, Tim J Schulz, Tilman Grune, Susanne Klaus, Anna P Kipp, Mario Ost
Mitochondrial dysfunction is usually associated with various metabolic disorders and ageing. However, salutary effects in response to mild mitochondrial perturbations have been reported in multiple organisms, whereas molecular regulators of cell-autonomous stress responses remain elusive. We addressed this question by asking whether the nuclear factor erythroid-derived-like 2 (Nrf2), a transcription factor and master regulator of cellular redox status is involved in adaptive physiological responses including muscle mitohormesis...
February 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29400110/scutellarin-ameliorates-nonalcoholic-fatty-liver-disease-through-the-ppar%C3%AE-pgc-1%C3%AE-nrf2-pathway
#7
Xiaoxue Zhang, Renpeng Ji, Huijun Sun, Jinyong Peng, Xiaodong Ma, ChangYuan Wang, Yufeng Fu, Liuchi Bao, Yue Jin
Nonalcoholic fatty liver disease (NAFLD) is characterised by excessive accumulation of hepatic lipids and oxidative injury of hepatocytes. Scutellarin is a flavonoid glycoside having antioxidative stress activity. Our current study aims to investigate the molecular mechanism of scutellarin ameliorating NAFLD. Scutellarin treatment was applied to male C57BL/6 mice maintained on a high-fat diet (HFD) and HepG2 cells challenged with oleic acid. The antioxidation biochemical indicators and lipid levels in the liver and cells were detected by kits...
February 5, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29394555/-a-case-of-ascending-colon-cancer-with-synchronous-liver-metastases-and-peritoneal-dissemination
#8
Masato Mito, Hitoshi Kameyama, Yoshifumi Shimada, Saki Yamada, Shinnosuke Hotta, Yuki Hirose, Ryoma Yagi, Yosuke Tajima, Mae Nakano, Takuma Okamura, Masato Nakano, Hiroshi Ichikawa, Masayuki Nagahashi, Jun Sakata, Toshifumi Wakai
The patient was a 73-year-old man with ascending colon cancer and synchronous liver metastases. A right hemicolectomy with a lymph node dissection was performed for the primary lesion. The resected specimen revealed a KRAS codon 12 mutation. After 6 courses of chemotherapy with capecitabine, oxaliplatin, and bevacizumab(Bv), we performed a partial hepatectomy and resection of the peritoneal dissemination. A computed tomography(CT)scan 5 months later revealed the recurrence of the liver metastases. After 8 courses of chemotherapy with 5-fluorouracil, Leucovorin, irinotecan, and Bv, we performed a partial hepatectomy...
November 2017: Gan to Kagaku Ryoho. Cancer & Chemotherapy
https://www.readbyqxmd.com/read/29383876/histone-methyltransferase-setd7-regulates-nrf2-signaling-pathway-by-phenethyl-isothiocyanate-and-ursolic-acid-in-human-prostate-cancer-cells
#9
Chao Wang, Limin Shu, Chengyue Zhang, Wenji Li, Renyi Wu, Yue Guo, Yuqing Yang, Ah-Ng Kong
SCOPE: This study aimed to investigate the role of the epigenetic regulator SET domain-containing lysine methyltransferase 7 (Setd7) in regulating the antioxidant Nrf2 pathway in prostate cancer (PCa) cells and examined the effects of two phytochemicals, phenethyl isothiocyanate (PEITC) and ursolic acid (UA). METHODS AND RESULTS: Lentivirus-mediated shRNA knockdown of Setd7 in LNCaP and PC-3 cells decreased the expression of downstream Nrf2 targets, such as NAD(P)H: quinone oxidoreductase 1 (Nqo1) and glutathione S-transferase theta 2 (Gstt2)...
January 30, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29373803/knockdown-of-nrf2-inhibits-angiogenesis-by-down-regulating-vegf-expression-through-pi3k-akt-signaling-pathway-in-cerebral-microvascular-endothelial-cells-under-hypoxic-conditions
#10
Yujing Huang, Ying Mao, Huiying Li, Guangxun Shen, Guangxian Nan
Ischemic stroke is a major cerebrovascular disease resulting from a transient or permanent local reduction of cerebral blood flow. Angiogenesis plays an important role in cerebral microvascular repair after ischemic stroke. This study aimed at investigating the effect of NF-E2-related factor 2 (Nrf2) on the angiogenesis of mouse cerebral microvascular endothelial bEnd.3 cells in a hypoxic environment. We found that Nrf2 expression was temporarily increased in hypoxia-induced bEnd.3 cells. Knockdown of Nrf2 inhibited the proliferation, migration as well as tube formation in hypoxia-induced bEnd...
January 26, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29363060/inflammation-oxidative-stress-and-higher-expression-levels-of-nrf2-and-nqo1-proteins-in-the-airways-of-women-chronically-exposed-to-biomass-fuel-smoke
#11
Nandan Kumar Mondal, Hirak Saha, Bidisha Mukherjee, Neetu Tyagi, Manas Ranjan Ray
The study was carried out to examine whether chronic exposure to smoke during daily household cooking with biomass fuel (BMF) elicits changes in airway cytology and expressions of Nrf2 (nuclear factor erythroid 2 [NF-E2]-related factor 2 [Nrf2]), Keap1 (Kelch-like erythroid-cell-derived protein with CNC homology [ECH]-associated protein 1), and NQO1 (NAD(P)H:quinone oxidoreductase 1) proteins in the airways. For this, 282 BMF-using women (median age 34 year) and 236 age-matched women who cooked with liquefied petroleum gas (LPG) were enrolled...
January 24, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29362432/nrf2-antioxidant-pathway-suppresses-numb-mediated-epithelial-mesenchymal-transition-during-pulmonary-fibrosis
#12
Zhihui Zhang, Jiao Qu, Cheng Zheng, Panpan Zhang, Wencheng Zhou, Wenhui Cui, Xiaoting Mo, Liucheng Li, Liang Xu, Jian Gao
Epithelial mesenchymal transition (EMT) is a key progression that promotes pulmonary fibrosis (PF). Numb, a phosphotyrosine-binding domain (PTB) protein, is implicated with EMT. Nuclear factor erythroid 2-related factor2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NAD(P)H: quinone oxidoreductase 1 (NQO1) constitute an important pathway of antioxidant defense signal for protecting against PF. It remains elusive whether Nrf2 antioxidant pathway and Numb have a potential relationship in EMT-mediated PF...
January 23, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29357726/metabolic-characteristics-of-tanshinone-i-in-human-liver-microsomes-and-s9-subcellular-fractions
#13
Yue Li, Yujuan Fan, Huizong Su, Qian Wang, Guofu Li, Yiyang Hu, Jian Jiang, Bo Tan, Furong Qiu
1. Tanshinone I (TSI) is a lipophilic diterpene in Salvia miltiorrhiza with versatile pharmacological activities. However, metabolic pathway of TSI in human is unknown. 2. In this study, we determined major metabolites of TSI using a preparation of human liver microsomes (HLMs) by HPLC-UV and Q-Trap mass spectrometer. A total of 6 metabolites were detected, which indicated the presence of hydroxylation, reduction as well as glucuronidation. 3. Selective chemical inhibition and purified cytochrome P450 (CYP450) isoform screening experiments revealed that CYP2A6 was primarily responsible for TSI Phase I metabolism...
January 23, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/29355553/new-application-of-the-commercial-sweetener-rebaudioside-a-as-a-hepatoprotective-candidate-induction-of-the-nrf2-signaling-pathway
#14
Yuxin Wang, Linyao Li, Yawen Wang, Xiaokang Zhu, Mingdong Jiang, Erqun Song, Yang Song
A large population of drug candidates have failed "from bench to bed" due to unwanted toxicities. We intend to develop an alternative approach for drug discovery, that is, to seek candidates from "safe" compounds. Rebaudioside A (Reb-A) is an approved commercial sweetener from Stevia rebaudiana Bertoni. We found that Reb-A protects against carbon tetrachloride (CCl4)-induced oxidative injury in human liver hepatocellular carcinoma (HepG2) cells. Reb-A showed antioxidant activity on reducing cellular reactive oxygen species and malondialdehyde levels while increasing glutathione levels and superoxide dismutase and catalase activities...
January 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29351230/comparison-of-antioxidative-effects-of-insect-tea-and-its-raw-tea-kuding-tea-polyphenols-in-kunming-mice
#15
Xin Zhao, Jia-Le Song, Ruokun Yi, Guijie Li, Peng Sun, Kun-Young Park, Huayi Suo
Kudingcha is a traditional Chinese tea, and insect tea is a special drink produced by the metabolism of insect larvae using the raw Kuding tea. Insect tea polyphenols (ITP) and its raw tea (Kuding tea) polyphenols (KTP) are high-purity polyphenols extracted by centrifuge precipitation. The present study was designed to compare the antioxidative effects of insect tea polyphenols (ITP) and its raw tea (Kuding tea) polyphenols (KTP) on d-galactose-induced oxidation in Kunming (KM) mice. KM mice were treated with ITP (200 mg/kg) and KTP (200 mg/kg) by gavage, and vitamin C (VC, 200 mg/kg) was also used as a positive control by gavage...
January 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29325897/an-improved-cell-permeable-fluorogenic-substrate-as-the-basis-for-a-highly-sensitive-test-for-nad-p-h-quinone-oxidoreductase-1-nqo1-in-living-cells
#16
Simone Cuff, Ruth D Lewis, Edwin Chinje, Mohammed Jaffar, Richard Knox, Ian Weeks
NAD(P)H:quinone oxidoreductase 1 (NQO1) is a flavoenzyme upregulated in response to oxidative stress and in some cancers. Its upregulation by compounds has been used as an indicator of their potential anti-cancer properties. In this study we have designed, produced and tested a fluorogenic coumarin conjugate which selectively releases highly fluorescent 4-methylumbelliferone (4-MU) in the presence of NQO1. It was found that measuring 4-MU release rapidly and specifically quantitated NQO1 levels in vitro and in live cells...
January 8, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29319808/intracellular-reduction-of-coenzyme-q-homologues-with-a-short-isoprenoid-side-chain-induces-apoptosis-of-hela-cells
#17
Takayuki Takahashi, Yukitoshi Mine, Tadashi Okamoto
Coenzyme Q (CoQ) is an essential factor of the mitochondrial respiratory chain. CoQ homologues with different lengths of the isoprenoid side chain are widely distributed in nature, but little is known about the relationship between the isoprenoid side chain length and biological function; therefore, we examined the effects of CoQ homologues on HeLa cells. When CoQ homologues with a shorter isoprenoid side chain than CoQ4 were added to HeLa cells, they induced cell death, and the order of cytotoxic intensity was as follows: CoQ0 ≫ CoQ3 ≈ CoQ1 > CoQ2 ≫ CoQ4...
January 8, 2018: Journal of Biochemistry
https://www.readbyqxmd.com/read/29318967/inter-individual-variability-in-activity-of-the-major-drug-metabolizing-enzymes-in-liver-homogenates-of-20-individuals
#18
J Chris Vos, Shalenie P den Braver-Sewradj, Michiel W den Braver, Marc van Dijk, Yongjie Zhang, Stefan J Dekker, Lukas Wijaya, Nico P E Vermeulen, Lysiane Richert, Jan N M Commandeur
BACKGROUND: Inter-individual variability in hepatic drug metabolizing enzyme (DME) activity is a major contributor to heterogeneity in drug clearance and safety. Accurate data on expression levels and activities of DMEs is an important prerequisite for in vitro-in vivo extrapolation and in silico based predictions. Characterization and assessment of inter-correlations of the major DMEs cytochrome P450s (CYPs) and UDP-glucuronosyltransferases (UGTs) have been extensively documented, but simultaneous quantification including other major DMEs has been lacking...
January 8, 2018: Current Drug Metabolism
https://www.readbyqxmd.com/read/29298345/redox-modulation-of-nqo1
#19
David Siegel, Donna D Dehn, Samantha S Bokatzian, Kevin Quinn, Donald S Backos, Andrea Di Francesco, Michel Bernier, Nichole Reisdorph, Rafael de Cabo, David Ross
NQO1 is a FAD containing NAD(P)H-dependent oxidoreductase that catalyzes the reduction of quinones and related substrates. In cells, NQO1 participates in a number of binding interactions with other proteins and mRNA and these interactions may be influenced by the concentrations of reduced pyridine nucleotides. NAD(P)H can protect NQO1 from proteolytic digestion suggesting that binding of reduced pyridine nucleotides results in a change in NQO1 structure. We have used purified NQO1 to demonstrate the addition of NAD(P)H induces a change in the structure of NQO1; this results in the loss of immunoreactivity to antibodies that bind to the C-terminal domain and to helix 7 of the catalytic core domain...
2018: PloS One
https://www.readbyqxmd.com/read/29291405/enhanced-glycometabolism-as-a-mechanism-of-nqo1-potentiated-growth-of-nsclc-revealed-by-metabolomic-profiling
#20
Xuefang Cheng, Fang Liu, Huiying Liu, Guangji Wang, Haiping Hao
NAD(P)H:quinone oxidoreductase 1 (NQO1), a cytoplasmic 2-electron reductase, has been considered as a potential poor prognostic biomarker and a promising therapeutic target for patients with non-small cell lung cancer (NSCLC) due to its frequent overexpression and significantly increased activity in NSCLC. Previous studies have shown that depleting tumor-NQO1 potentiates anoikis and inhibits growth of NSCLC. However, the underlying mechanisms whereby NQO1 potentiates proliferation have not been fully understood...
December 29, 2017: Biochemical and Biophysical Research Communications
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